MD05G1220200.v1.1

G-type lectin S-receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000484
Physical Location & Seq
Reverse (-)
35298291 .. 35301755
3465 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1220200.v1.1.491

Sequence Viewer

Length: 2490 bp
ATGAATTTAATGAAAAATCCATTGAAAGCTTCAACTAAGATGTGCTTGATCCTCTTCTACTCTTCTTTGCTGCTCATCTTCGCAGTAGTATTCTCCATAGCTGATGACGCCACGAGTACATTTCAGTCCATTAGAGACGGTGAGACTGTAGTTTCCACTGGCGGTACGTTTGAGCTAGGATTTTGGAGTCCTGATGCTTCTAATAGACGGTACGTGGGGATATGGTACAAGCACATATCTGTTACAACCATTGTATGGGTTGCCAACAGAGACAAGCCCCTCCTCGATTTGTCCGGCGTTCTGAAGGTCACCAACCCCGGAATTCTTGTCCTCAACCACAGCATGAGCACAGTTTGGTCCTCCAACACATCGAGAACTGCACAGAATCCAGTGGCACGTCTTTTGGATTCGGGTAATCTTGTCGTGATAGATAGGAGTGATGATGACTCTGAGAACTTTCTGTGGCAAAGTTTTGATTACCCTGGCGATACATTCTTACCAGGTATGAAGATTGGTAGGAACACAGTTACAGGCTTCAATTGGCATCTTAGATCATGGAAAAGTCCTCAGGATCCTTCTCAAGGAAATCGTACATCTCAACTTGGTCCCAAAGGATATGCAGAATTATTTGTGAGGGAAGGTTCGGTCATTAAATTTCGTTCTGGACCATGGAATGGAGTCCGGTTCAGTGGAAACCCTCAGTTAAATCCAAACCCTATATACACATACGGTCTTGTTTCTGAGCCTGATGAAATGTACTACAGTTACAAGCTTCGCAACAGCTCAATCTTTTCGAGGTTGGTGTTAACTTCAGATGGACATGTGCAGCGCTACACATGGATTGATAGAACCAAAGGTTGGGATCTTTACCTAACAGCCCAAATTGATAATTGTGACAAGTATGCATCCTGTGGTGTATATGGTGCATGTAACATTGAAAAGTCCCCGGTTTGTAGTTGTTTGAAAGGATTTACACCAAAGTTTCCGAATGAATGGGATTTGGTAGATTGGTCTAATGGTTGCGTGAGAAAGACGTCTCTAAATTGCACCGGAGATGTGTTCCAAAAGTACTCCGGGTTGAAATTGCCTAGCACAGAACAATCCTGGTTTAACAAAAGTATGAACCTCAAGGAATGTGAGATGGTGTGCATGAAGAACTGCTCCTGCACGGCTTATACAAATTTGGATATCCGGGATGGAGGAACTGGGTGCTTGCTGTGGTACGGTGCTCTAATTGATATAAGATACTTTGCTGAAAACGGGCAAGATATTTATATAAGAATGGCCGCAGCAGAACAAGATCATGAAGACGATACAAAGATCAACGCTAAATACTCTAAATCCAATGCAAAGAAAATGAGAATCATAATAAGCACTACTGTGTTGTCTACCGGACTGCTGATCCTGGGCCTTGCCCTGTTGTTTTATGTTTGGAAGAAGAAGCACCAAAAAGGTGGAAAACTAGGACGCAGCCAAAAGGAGGATATGGAATTACCGTTATTTGACTTGATGACTATAGTTTCTGCAACCAGTAACTTTTCAATTGAAAACAAACTTGGTGAAGGCGGTTTCGGATCTGTCTTTAAGGGTACGATGGAAGATGGACAAGAAATCGCTGTGAAAAGGCTTTCAAAAAGTTCTAGACAAGGACTTGATGAGTTCAAGAATGAAGTTACACATATTGCCAAACTTCAGCACAGGAATCTAGTGAAGCTTCTTGGATGCTGTATTCAAGAAGACGAGATGATATTAATCTACGAGTTCATGCCTAACAAGAGCTTAGACTTTTTTATTTTCGATCAAAGAAGAAGGATGTTATTAGACTGGCCGAAGCGCTTTGAAATTATTAATGGGATAGCTCGAGGGCTCCTCTATCTTCATCAAGATTCTAGATTGAGAGTTGTTCATAGAGATCTCAAAGCAAGTAACATTTTATTAGGCAGTGAATTTAACCCGAAAATCTCAGACTTTGGCCTGGCTAGAAGCTTTGGAGGAAATGAAACGAAAGCAGAGACGAAGAAAGTGGTTGGAACATACGGTTACATGTCCCCAGAATATGCAATTGATGGTTTCTACTCTACAAAGTCCGACGTCTATAGCTTTGGTGTTCTGGTGCTAGAGATAGTGAGTGGGAACAGAAATAGAGGATTCTCTCATCCAAACCACAAACTCAACCTTCTTGGACATGCATGGATGCTACACACAGAAGGCAGACCTCTCGAACTGCTTGATACATCGGTAGAGGACTCCATCACTCTGCTTGAAGTTGTGAGAACGATTCATGTGGGTCTTTTGTGCGTGCAGCGAAATCCAGAGGACAGGCCGAGCATGTCAGCTGCAGTTCTAATGTTGAGTGGTGAAGGTGCATTGCCTCAACCTTTGAAACCTGGTTTTTACAGTGAAAGGGATCTGACTGAACTCGAAGCTAGTCATTCTTCTAAAGCATGCTCGGCTAATGAAGTCACTATTTCAGTACTTGAGGCTCGATAA

Protein Analysis

830

Amino Acids

93.2

Weight (kDa)

8.14

Isoelectric Point (pI)

41.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 83 - 187 1.2e-37 D-mannose binding lectin
S_locus_glycop PF00954 219 - 328 2.1e-32 S-locus glycoprotein domain
PAN_2 PF08276 349 - 414 3.7e-19 PAN-like domain
PK_Tyr_Ser-Thr PF07714 514 - 779 9.6e-48 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 514 - 779 1.2e-45 Protein kinase domain
DUF3403 PF11883 787 - 829 2.8e-08 Domain of unknown function (DUF3403)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000484)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27290 AT4G27290
fragaria_vesca FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g21310 FvH4_3g21310
malus_domestica MD05G1219900.v1.1 MD05G1220200.v1.1 MD05G1230800.v1.1 MD05G1231100.v1.1 MD05G1231700.v1.1 MD05G1232100.v1.1 MD05G1232400.v1.1 MD05G1233000.v1.1 MD10G1202800.v1.1
prunus_persica Prupe.4G139500_v2.0.a1 Prupe.4G139500_v2.0.a1
pyrus_communis pycom05g20170 pycom05g20200 pycom05g20230 pycom05g20250 pycom05g20260 pycom05g20280 pycom05g20320 pycom05g20370 pycom05g20380 pycom05g24850 pycom10g17340
rosa_chinensis RchiOBHm_Chr5g0026351 RchiOBHm_Chr5g0026371 RchiOBHm_Chr5g0026381 RchiOBHm_Chr5g0026411 RchiOBHm_Chr5g0026551 RchiOBHm_Chr5g0036371
rosa_laevigata RLG00000032923 RLG00000032926 RLG00000032927 RLG00000032928 RLG00000032934 RLG00000033692
rosa_multiflora Rmu_co8389779.1_g000001 Rmu_sc0000712.1_g000012 Rmu_sc0001017.1_g000026 Rmu_sc0001114.1_g000001 Rmu_sc0001114.1_g000002 Rmu_sc0001114.1_g000008 Rmu_sc0005581.1_g000006 Rmu_sc0005581.1_g000007 Rmu_sc0006059.1_g000052 Rmu_sc0042966.1_g000001
rosa_roxburghii Rroxscaffold_1G00044440 Rroxscaffold_1G00052930 Rroxscaffold_1G00052980 Rroxscaffold_1G00052990 Rroxscaffold_1G00053000
rosa_rugosa Rorug05G0092500 Rorug05G0092500 Rorug05G0092500 Rorug05G0092600 Rorug05G0092700 Rorug05G0092700 Rorug05G0093700 Rorug05G0155200 Rorug05G0155800 Rorug05G0155800
rosa_samantha Rh5AG185700 Rh5AG186300 Rh5BG182600 Rh5BG182900 Rh5BG183600 Rh5BG249400 Rh5CG202000 Rh5CG202100 Rh5CG202200 Rh5CG203400 Rh5CG281200 Rh5DG184400 Rh5DG184500 Rh5DG185900
rosa_wichuraiana Rw5G016850 Rw5G016870 Rw5G016930 Rw5G022870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 1037, 2094
AccB7I CCANNNNNTGG 3 cut(s) 255, 674, 858
AccI GTMKAC 1 cut(s) 1388
AciI CCGC 3 cut(s) 162, 1287, 1566
AclWI GGATC 7 cut(s) 43, 566, 579, 870, 1396, 1582, 2415
AcoI YGGCCR 2 cut(s) 1284, 1826
AcsI RAATTY 5 cut(s) 4, 321, 653, 1180, 1946
AcuI CTGAAG 3 cut(s) 323, 795, 1676
AcyI GRCGYC 3 cut(s) 108, 1034, 2091
AfeI AGCGCT 2 cut(s) 830, 1835
AfiI CCNNNNNNNGG 5 cut(s) 255, 581, 674, 858, 1480
AflIII ACRYGT 2 cut(s) 820, 2043
AjiI CACGTC 1 cut(s) 398
AjnI CCWGG 6 cut(s) 481, 499, 1103, 1404, 1974, 2386
AjuI GAANNNNNNNTTGG 4 cut(s) 841, 873, 1539, 1571
AleI CACNNNNGTG 1 cut(s) 1379
Alw21I GWGCWC 2 cut(s) 350, 1231
Alw26I GTCTC 5 cut(s) 129, 137, 264, 1041, 2006
AlwI GGATC 7 cut(s) 43, 566, 579, 870, 1396, 1582, 2415
Ama87I CYCGRG 1 cut(s) 1860
Aor51HI AGCGCT 2 cut(s) 830, 1835
AoxI GGCC 5 cut(s) 1284, 1408, 1826, 1972, 2321
ApeKI GCWGC 6 cut(s) 70, 826, 1289, 1470, 2302, 2336
ApoI RAATTY 5 cut(s) 4, 321, 653, 1180, 1946
AseI ATTAAT 2 cut(s) 1751, 1848
AspLEI GCGC 2 cut(s) 831, 1836
AspS9I GGNCC 4 cut(s) 357, 605, 665, 1408
AsuC2I CCSGG 4 cut(s) 318, 947, 1075, 1193
AsuHPI GGTGA 4 cut(s) 152, 301, 1571, 2369
AvaI CYCGRG 1 cut(s) 1860
AvaII GGWCC 3 cut(s) 357, 605, 665
AxyI CCTNAGG 1 cut(s) 567
BaeI ACNNNNGTAYC 2 cut(s) 2223, 2256
BamHI GGATCC 1 cut(s) 571
BanII GRGCYC 1 cut(s) 1869
BauI CACGAG 1 cut(s) 112
BbsI GAAGAC 2 cut(s) 1314, 1743
Bbv12I GWGCWC 2 cut(s) 350, 1231
BbvI GCAGC 6 cut(s) 57, 838, 1301, 1482, 2314, 2323
BccI CCATC 7 cut(s) 809, 1135, 1190, 1588, 1595, 2060, 2258
BceAI ACGGC 1 cut(s) 1185
BcgI CGANNNNNNTGC 2 cut(s) 2200, 2234
BciT130I CCWGG 6 cut(s) 483, 501, 1105, 1406, 1976, 2388
BcnI CCSGG 4 cut(s) 318, 947, 1075, 1193
BcoDI GTCTC 5 cut(s) 129, 137, 264, 1041, 2006
BfaI CTAG 9 cut(s) 176, 1089, 1463, 1641, 1706, 1890, 1980, 2117, 2427
BfmI CTRYAG 5 cut(s) 147, 760, 1515, 2095, 2337
BfoI RGCGCY 2 cut(s) 832, 1837
BglII AGATCT 1 cut(s) 1912
BisI GCNGC 7 cut(s) 71, 827, 1287, 1290, 1471, 2303, 2337
BlsI GCNGC 7 cut(s) 72, 828, 1288, 1291, 1472, 2304, 2338
BmcAI AGTACT 2 cut(s) 1070, 2475
Bme18I GGWCC 3 cut(s) 357, 605, 665
BmeT110I CYCGRG 1 cut(s) 1860
BmgBI CACGTC 1 cut(s) 398
BmgT120I GGNCC 4 cut(s) 357, 605, 665, 1408
BmiI GGNNCC 3 cut(s) 573, 607, 1868
BmrI ACTGGG 1 cut(s) 1215
BmsI GCATC 5 cut(s) 184, 553, 914, 1712, 2184
BmuI ACTGGG 1 cut(s) 1215
BpiI GAAGAC 2 cut(s) 1314, 1743
BplI GAGNNNNNCTC 2 cut(s) 1854, 1886
BpuEI CTTGAG 2 cut(s) 564, 1112
BpuMI CCSGG 4 cut(s) 318, 947, 1075, 1193
BsaAI YACGTR 1 cut(s) 214
BsaBI GATNNNNATC 1 cut(s) 1751
BsaHI GRCGYC 3 cut(s) 108, 1034, 2091
BsaJI CCNNGG 5 cut(s) 316, 481, 668, 945, 1405
BsaWI WCCGGW 3 cut(s) 681, 1049, 1391
Bsc4I CCNNNNNNNGG 5 cut(s) 255, 581, 674, 858, 1480
Bse1I ACTGG 5 cut(s) 163, 389, 1210, 1530, 1829
Bse21I CCTNAGG 1 cut(s) 567
Bse3DI GCAATG 1 cut(s) 2366
Bse8I GATNNNNATC 1 cut(s) 1751
BseBI CCWGG 6 cut(s) 483, 501, 1105, 1406, 1976, 2388
BseDI CCNNGG 5 cut(s) 316, 481, 668, 945, 1405
BseGI GGATG 6 cut(s) 905, 1201, 1727, 1818, 2155, 2199
BseJI GATNNNNATC 1 cut(s) 1751
BseLI CCNNNNNNNGG 5 cut(s) 255, 581, 674, 858, 1480
BseMI GCAATG 1 cut(s) 2366
BseMII CTCAG 5 cut(s) 441, 581, 713, 732, 1977
BseNI ACTGG 5 cut(s) 163, 389, 1210, 1530, 1829
BseRI GAGGAG 2 cut(s) 272, 1859
BseXI GCAGC 6 cut(s) 57, 838, 1301, 1482, 2314, 2323
BsgI GTGCAG 4 cut(s) 363, 845, 1150, 2321
BshFI GGCC 5 cut(s) 1286, 1410, 1828, 1974, 2323
BsiHKAI GWGCWC 2 cut(s) 350, 1231
BsiHKCI CYCGRG 1 cut(s) 1860
BsiSI CCGG 8 cut(s) 294, 318, 682, 947, 1050, 1074, 1192, 1392
BslFI GGGAC 3 cut(s) 591, 928, 2032
BslI CCNNNNNNNGG 5 cut(s) 255, 581, 674, 858, 1480
BsmAI GTCTC 5 cut(s) 129, 137, 264, 1041, 2006
BsmBI CGTCTC 3 cut(s) 129, 1041, 2006
BsmFI GGGAC 3 cut(s) 591, 928, 2032
BsnI GGCC 5 cut(s) 1286, 1410, 1828, 1974, 2323
BsoBI CYCGRG 1 cut(s) 1860
Bsp1286I GDGCHC 3 cut(s) 350, 1231, 1869
Bsp19I CCATGG 1 cut(s) 668
BspACI CCGC 3 cut(s) 162, 1287, 1566
BspANI GGCC 5 cut(s) 1286, 1410, 1828, 1974, 2323
BspCNI CTCAG 5 cut(s) 442, 580, 712, 733, 1976
BspHI TCATGA 1 cut(s) 1303
BspLI GGNNCC 3 cut(s) 573, 607, 1868
BspMAI CTGCAG 1 cut(s) 2341
BspPI GGATC 7 cut(s) 43, 566, 579, 870, 1396, 1582, 2415
BsrDI GCAATG 1 cut(s) 2366
BsrI ACTGG 5 cut(s) 163, 389, 1210, 1530, 1829
BssECI CCNNGG 5 cut(s) 316, 481, 668, 945, 1405
BssNI GRCGYC 3 cut(s) 108, 1034, 2091
BssSI CACGAG 1 cut(s) 112
BssT1I CCWWGG 1 cut(s) 668
Bst2BI CACGAG 1 cut(s) 112
Bst2UI CCWGG 6 cut(s) 483, 501, 1105, 1406, 1976, 2388
Bst6I CTCTTC 2 cut(s) 59, 67
BstACI GRCGYC 3 cut(s) 108, 1034, 2091
BstBAI YACGTR 1 cut(s) 214
BstC8I GCNNGC 3 cut(s) 1214, 2300, 2446
BstDEI CTNAG 8 cut(s) 36, 450, 548, 567, 699, 741, 1780, 1963
BstDSI CCRYGG 1 cut(s) 668
BstEII GGTNACC 1 cut(s) 307
BstENI CCTNNNNNAGG 1 cut(s) 579
BstF5I GGATG 6 cut(s) 905, 1201, 1727, 1818, 2155, 2199
BstH2I RGCGCY 2 cut(s) 832, 1837
BstHHI GCGC 2 cut(s) 831, 1836
BstMAI GTCTC 5 cut(s) 129, 137, 264, 1041, 2006
BstMWI GCNNNNNNNGC 2 cut(s) 107, 2450
BstNI CCWGG 6 cut(s) 483, 501, 1105, 1406, 1976, 2388
BstNSI RCATGY 6 cut(s) 824, 930, 2047, 2189, 2332, 2448
BstPI GGTNACC 1 cut(s) 307
BstSFI CTRYAG 5 cut(s) 147, 760, 1515, 2095, 2337
BstV1I GCAGC 6 cut(s) 57, 838, 1301, 1482, 2314, 2323
BstV2I GAAGAC 2 cut(s) 1314, 1743
BstX2I RGATCY 5 cut(s) 571, 862, 1574, 1912, 2407
BstXI CCANNNNNNTGG 1 cut(s) 1454
BstYI RGATCY 5 cut(s) 571, 862, 1574, 1912, 2407
Bsu36I CCTNAGG 1 cut(s) 567
BsuRI GGCC 5 cut(s) 1286, 1410, 1828, 1974, 2323
BtgI CCRYGG 1 cut(s) 668
BtrI CACGTC 1 cut(s) 398
BtsCI GGATG 6 cut(s) 905, 1201, 1727, 1818, 2155, 2199
BtsI GCAGTG 1 cut(s) 1948
BtsIMutI CAGTG 5 cut(s) 156, 396, 694, 1948, 2404
Cac8I GCNNGC 3 cut(s) 1214, 2300, 2446
CciI TCATGA 1 cut(s) 1303
CfoI GCGC 2 cut(s) 831, 1836
Cfr13I GGNCC 4 cut(s) 357, 605, 665, 1408
CseI GACGC 2 cut(s) 116, 1476
CsiI ACCWGGT 2 cut(s) 499, 2386
DdeI CTNAG 8 cut(s) 36, 450, 548, 567, 699, 741, 1780, 1963
EaeI YGGCCR 2 cut(s) 1284, 1826
Eam1104I CTCTTC 2 cut(s) 59, 67
EarI CTCTTC 2 cut(s) 59, 67
Eco130I CCWWGG 1 cut(s) 668
Eco24I GRGCYC 1 cut(s) 1869
Eco32I GATATC 1 cut(s) 1189
Eco47I GGWCC 3 cut(s) 357, 605, 665
Eco47III AGCGCT 2 cut(s) 830, 1835
Eco57I CTGAAG 3 cut(s) 323, 795, 1676
Eco81I CCTNAGG 1 cut(s) 567
Eco88I CYCGRG 1 cut(s) 1860
Eco91I GGTNACC 1 cut(s) 307
EcoNI CCTNNNNNAGG 1 cut(s) 579
EcoO65I GGTNACC 1 cut(s) 307
EcoRI GAATTC 1 cut(s) 321
EcoRII CCWGG 6 cut(s) 481, 499, 1103, 1404, 1974, 2386
EcoRV GATATC 1 cut(s) 1189
EcoT14I CCWWGG 1 cut(s) 668
EcoT22I ATGCAT 2 cut(s) 907, 2191
EcoT38I GRGCYC 1 cut(s) 1869
ErhI CCWWGG 1 cut(s) 668
Esp3I CGTCTC 3 cut(s) 129, 1041, 2006
FalI AAGNNNNNCTT 2 cut(s) 29, 61
FaqI GGGAC 3 cut(s) 591, 928, 2032
FblI GTMKAC 1 cut(s) 1388
Fnu4HI GCNGC 7 cut(s) 71, 827, 1287, 1290, 1471, 2303, 2337
FokI GGATG 6 cut(s) 892, 1208, 1734, 1825, 2142, 2206
FriOI GRGCYC 1 cut(s) 1869
Fsp4HI GCNGC 7 cut(s) 71, 827, 1287, 1290, 1471, 2303, 2337
FspBI CTAG 9 cut(s) 176, 1089, 1463, 1641, 1706, 1890, 1980, 2117, 2427
GlaI GCGC 2 cut(s) 830, 1835
GluI GCNGC 7 cut(s) 71, 827, 1287, 1290, 1471, 2303, 2337
HaeII RGCGCY 2 cut(s) 832, 1837
HaeIII GGCC 5 cut(s) 1286, 1410, 1828, 1974, 2323
HapII CCGG 8 cut(s) 294, 318, 682, 947, 1050, 1074, 1192, 1392
HgaI GACGC 2 cut(s) 116, 1476
HhaI GCGC 2 cut(s) 831, 1836
Hin1I GRCGYC 3 cut(s) 108, 1034, 2091
Hin6I GCGC 2 cut(s) 829, 1834
HinP1I GCGC 2 cut(s) 829, 1834
HincII GTYRAC 1 cut(s) 807
HindII GTYRAC 1 cut(s) 807
HindIII AAGCTT 4 cut(s) 27, 770, 1712, 1984
HpaI GTTAAC 1 cut(s) 807
HpaII CCGG 8 cut(s) 294, 318, 682, 947, 1050, 1074, 1192, 1392
HphI GGTGA 4 cut(s) 152, 301, 1571, 2369
Hpy166II GTNNAC 2 cut(s) 807, 1389
Hpy188I TCNGA 9 cut(s) 303, 451, 742, 814, 987, 1574, 1966, 2089, 2412
Hpy8I GTNNAC 2 cut(s) 807, 1389
Hpy99I CGWCG 1 cut(s) 2093
HpyAV CCTTC 8 cut(s) 298, 585, 632, 1556, 1803, 2186, 2201, 2354
HpyCH4IV ACGT 5 cut(s) 167, 213, 397, 1034, 2091
HpyF10VI GCNNNNNNNGC 2 cut(s) 107, 2450
HpyF3I CTNAG 8 cut(s) 36, 450, 548, 567, 699, 741, 1780, 1963
HpySE526I ACGT 5 cut(s) 167, 213, 397, 1034, 2091
Hsp92I GRCGYC 3 cut(s) 108, 1034, 2091
HspAI GCGC 2 cut(s) 829, 1834
KspAI GTTAAC 1 cut(s) 807
LmnI GCTCC 2 cut(s) 1166, 1872
Lsp1109I GCAGC 6 cut(s) 57, 838, 1301, 1482, 2314, 2323
LweI GCATC 5 cut(s) 184, 553, 914, 1712, 2184
MabI ACCWGGT 2 cut(s) 499, 2386
MaeI CTAG 9 cut(s) 176, 1089, 1463, 1641, 1706, 1890, 1980, 2117, 2427
MaeII ACGT 5 cut(s) 167, 213, 397, 1034, 2091
MfeI CAATTG 3 cut(s) 538, 1542, 2061
MflI RGATCY 5 cut(s) 571, 862, 1574, 1912, 2407
MhlI GDGCHC 3 cut(s) 350, 1231, 1869
MlyI GAGTC 4 cut(s) 196, 440, 687, 2240
MmeI TCCRAC 3 cut(s) 387, 2008, 2112
Mph1103I ATGCAT 2 cut(s) 907, 2191
MseI TTAA 9 cut(s) 8, 651, 704, 806, 1110, 1584, 1751, 1848, 1950
MslI CAYNNNNRTG 1 cut(s) 1379
MspA1I CMGCKG 1 cut(s) 2336
MspI CCGG 8 cut(s) 294, 318, 682, 947, 1050, 1074, 1192, 1392
MunI CAATTG 3 cut(s) 538, 1542, 2061
MvaI CCWGG 6 cut(s) 483, 501, 1105, 1406, 1976, 2388
MwoI GCNNNNNNNGC 2 cut(s) 107, 2450
NciI CCSGG 4 cut(s) 318, 947, 1075, 1193
NcoI CCATGG 1 cut(s) 668
NlaIV GGNNCC 3 cut(s) 573, 607, 1868
NmeAIII GCCGAG 2 cut(s) 2349, 2429
NmuCI GTSAC 3 cut(s) 307, 893, 2461
NsiI ATGCAT 2 cut(s) 907, 2191
NspI RCATGY 6 cut(s) 824, 930, 2047, 2189, 2332, 2448
OliI CACNNNNGTG 1 cut(s) 1379
PaeI GCATGC 1 cut(s) 2448
PaeR7I CTCGAG 1 cut(s) 1860
PagI TCATGA 1 cut(s) 1303
PciI ACATGT 2 cut(s) 820, 2043
PfeI GAWTC 7 cut(s) 385, 407, 1362, 1702, 1886, 2148, 2278
PflMI CCANNNNNTGG 3 cut(s) 255, 674, 858
PfoI TCCNGGA 1 cut(s) 1191
PkrI GCNGC 7 cut(s) 72, 828, 1288, 1291, 1472, 2304, 2338
PleI GAGTC 4 cut(s) 195, 440, 686, 2240
PpsI GAGTC 4 cut(s) 195, 440, 686, 2240
Ppu21I YACGTR 1 cut(s) 214
PscI ACATGT 2 cut(s) 820, 2043
PshBI ATTAAT 2 cut(s) 1751, 1848
Psp6I CCWGG 6 cut(s) 481, 499, 1103, 1404, 1974, 2386
PspEI GGTNACC 1 cut(s) 307
PspGI CCWGG 6 cut(s) 481, 499, 1103, 1404, 1974, 2386
PspN4I GGNNCC 3 cut(s) 573, 607, 1868
PspPI GGNCC 4 cut(s) 357, 605, 665, 1408
PspXI VCTCGAGB 1 cut(s) 1860
PstI CTGCAG 1 cut(s) 2341
PsuI RGATCY 5 cut(s) 571, 862, 1574, 1912, 2407
PvuII CAGCTG 1 cut(s) 2336
RseI CAYNNNNRTG 1 cut(s) 1379
SaqAI TTAA 9 cut(s) 8, 651, 704, 806, 1110, 1584, 1751, 1848, 1950
SatI GCNGC 7 cut(s) 71, 827, 1287, 1290, 1471, 2303, 2337
Sau96I GGNCC 4 cut(s) 357, 605, 665, 1408
ScaI AGTACT 2 cut(s) 1070, 2475
SchI GAGTC 4 cut(s) 196, 440, 687, 2240
SduI GDGCHC 3 cut(s) 350, 1231, 1869
SexAI ACCWGGT 2 cut(s) 499, 2386
SfaNI GCATC 5 cut(s) 184, 553, 914, 1712, 2184
SfcI CTRYAG 5 cut(s) 147, 760, 1515, 2095, 2337
Sfr274I CTCGAG 1 cut(s) 1860
SinI GGWCC 3 cut(s) 357, 605, 665
SlaI CTCGAG 1 cut(s) 1860
SmiMI CAYNNNNRTG 1 cut(s) 1379
SmlI CTYRAG 4 cut(s) 579, 1127, 1860, 2477
SmoI CTYRAG 4 cut(s) 579, 1127, 1860, 2477
SphI GCATGC 1 cut(s) 2448
SsiI CCGC 3 cut(s) 162, 1287, 1566
SspMI CTAG 9 cut(s) 176, 1089, 1463, 1641, 1706, 1890, 1980, 2117, 2427
StyI CCWWGG 1 cut(s) 668
TaiI ACGT 5 cut(s) 170, 216, 400, 1037, 2094
TaqI TCGA 8 cut(s) 285, 371, 794, 1797, 1861, 2220, 2421, 2485
TaqII GACCGA 1 cut(s) 634
TatI WGTACW 4 cut(s) 116, 756, 1068, 2473
TauI GCSGC 1 cut(s) 1289
TfiI GAWTC 7 cut(s) 385, 407, 1362, 1702, 1886, 2148, 2278
Tru1I TTAA 9 cut(s) 8, 651, 704, 806, 1110, 1584, 1751, 1848, 1950
Tru9I TTAA 9 cut(s) 8, 651, 704, 806, 1110, 1584, 1751, 1848, 1950
TscAI CASTG 5 cut(s) 163, 396, 694, 1948, 2404
TseFI GTSAC 3 cut(s) 307, 893, 2461
TseI GCWGC 6 cut(s) 70, 826, 1289, 1470, 2302, 2336
Tsp45I GTSAC 3 cut(s) 307, 893, 2461
TspRI CASTG 5 cut(s) 163, 396, 694, 1948, 2404
Van91I CCANNNNNTGG 3 cut(s) 255, 674, 858
VpaK11BI GGWCC 3 cut(s) 357, 605, 665
VspI ATTAAT 2 cut(s) 1751, 1848
XagI CCTNNNNNAGG 1 cut(s) 579
XapI RAATTY 5 cut(s) 4, 321, 653, 1180, 1946
XbaI TCTAGA 2 cut(s) 1640, 1889
XceI RCATGY 6 cut(s) 824, 930, 2047, 2189, 2332, 2448
XhoI CTCGAG 1 cut(s) 1860
XmiI GTMKAC 1 cut(s) 1388
XspI CTAG 9 cut(s) 176, 1089, 1463, 1641, 1706, 1890, 1980, 2117, 2427
ZraI GACGTC 2 cut(s) 1035, 2092
ZrmI AGTACT 2 cut(s) 1070, 2475
Zsp2I ATGCAT 2 cut(s) 907, 2191
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.