Rroxscaffold_1G00052980

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000484
Physical Location & Seq
Reverse (-)
73726635 .. 73729740
3106 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00052980.1

Sequence Viewer

Length: 2349 bp
ATGCTTGCCACAGCAGAAGCCAGCACAAGTACATTTCAGTCCATCAAAGACGGCGAGACCATAGTTTCAACTGATGGAACCTTTGAGCTCGGATTCTTCACTCCTGCAGGCGCTTCTGGTAATCGATACGTAGGCATATGGTACAAGAAGATATCGGTTACAACGGTTGTGTGGGTTGCCAACAGAGAAACACCCCTCACTGATTCATCAGGTATTCTCAGAATCACTAACCCTGGAATTCTTATCCTTGTCAACCAGAACAGCGCCACAGTTTGGTCCTCCAACACATCAACAACCACACCAAATGCAGTGGCACAGCTTTTGGATTCAGGAAACCTTGTTGTGAAAGATGGCAGTGACAAAGTTGATCCAGAGAATGAGAAGTATTTGTGGCAGAGTTTTGACTACCCTGGTGATACATTGCTAGCAGGTATGAAGCTTGGTAGGAACACAGTTACAGGCTTCAATTGGCATCTTACATCATGGAGAAGTCCCTCTGATCCTTCTCAAGGCAACTTCACATTTCAACATGGTCCCGGGGGATATGCAGAACAAGTTCTGAGGGAGGGTTCGGACATAAGATTTCGGACTGGACCATGGAATGGAATCCGGTTTAGTGGAACGCCTCATTTAGGTCCAAACTATGTTTACACATATCAGCTAGTTTTTGATGATCATGATCATGAAGAATACTATAGCTATAAGCTTCTCAACAGCTCAGTTCTTTCCAGGCTGGTGTTAACTCAAGATGGGCTTCTGCAGCGCTACACATGGATCGATAGAACCCAAAGTTGGTTCCTTTACTTAACAGCACAGATGGATAACTGTGACAATTATGCACTTTGTGGTGCATATGGTGCTTGCAATATTGAGAACTCCCCAGTATGCGGCTGCTTGAAAGGTTTTGTGCCAAAATTTCCAAAAGATTGGGACACAATGGATTGGTCAAATGGGTGTGTGAGAAAGACTCCACTAGCTTGCAATGGCGACAAGTTTCTGAAGTACTCTGGGGTCAAATTTCCAAACACAGAACAATCCTGGTTTAACAAAAGTATGAGCCTCAAGGAATGTGAGATGGAGTGCACCAGAAACTGTTCCTGCACTGCTTATTCAAATTTGTATATTAGCGAAGGAGGAACTGGGTGCTTGATGTGGTTTGGCGATCTGATTGATATGAGAAATCTCACTGAAAATGGACAAGATATTTACATAAGGATGGCAGCATCAGAACAAGATCCTGAAGACTACACAAAGATCAATGGTAAACTTGCTAATTTGAATGTGAAGAAAAGGAGGATCATATTAAGCTGTATCGTGCCATCTACTGGACTGCTGATCCTGGGCCTAGCCTTCTGTCTATACATTTGCCAGAAGAAACATAAAAAAGATGACTGCACGGTATGGACTTTGGTCATAATTAAGCGAACTGTTGGATTTCAGGGTATGTTGAGAAATGGGCAAGAAATAGCTGTCAAAAGGCTCTCAAAACATTCTTCACAAGGAGTAAATGAGTTCAAAAATGAGGTTACGCATATTGCCAAACTCCAGCACAGGAATCTAGTGAAGCTTCTTGGATGTTGCATTCAAGCCGATGAAATGATGCTCATCTACGAATGCATGCCTAACAAAAGCTTGGACTTCTTTATTTTTGATCAAAGAAAAAGCATGCTACTAGATTGGCCTAAGCGCTTTGACATCATCAATGGTATTGCTCGGGGGCTCCTCTATCTTCATCAAGATTCTAGACTGAGAGTAATTCATAGAGATCTCAAAGCAGGCAATATTTTATTAGACAATGAATTTAACCCCAAGATCTCAGACTTTGGTCTAGCTAGAAGTTTTGGAGGAAATGAAACTGTAGCAGAAACGATGATAGTGGTTGGAACCTATGGTTACATGTCCCCAGAATATGCAAGTGACGGGTTCTACTCTACCAAGTCCGACGTCTTTAGCTTTGGTGTTTCGGTGTTGGAGATAATAAGTGGGAAGAGAAATAGAGGATTCTCTCATCCAGACCACGACCTCAACCTTCTTGGACATGCATGGAATCTATACACGGAAGGCAGGTCCATAGAATTGCTTGATACATCAGTAGGGAACTCCACTAATCCATCTGAGGAAGTTCTGCGCTCAATTCATGTGGGTCTTTTATGTGTGCAGAAAGATCCAGAAGATAGGCCAAGCATGTCAGATGTTGTTCTAATGTTGAGTGGTGAAGGTGCATTGCCTCAACCTGAAAAACCTGGGTTTTATAGTAAAAGTAGTGATAGGTCTGAAAACCAAGTCAATCCTTTATCTAGAGCATGCTCGGCTAATGAAGTTAGTTTTACGCTACTTGAGGCTAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

782

Amino Acids

87.6

Weight (kDa)

6.07

Isoelectric Point (pI)

42.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 55 - 163 3.3e-37 D-mannose binding lectin
S_locus_glycop PF00954 195 - 306 3.1e-33 S-locus glycoprotein domain
PAN_2 PF08276 327 - 392 4.1e-20 PAN-like domain
Pkinase PF00069 484 - 733 1.8e-39 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 485 - 734 1.4e-41 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000484)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27290 AT4G27290
fragaria_vesca FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g21310 FvH4_3g21310
malus_domestica MD05G1219900.v1.1 MD05G1220200.v1.1 MD05G1230800.v1.1 MD05G1231100.v1.1 MD05G1231700.v1.1 MD05G1232100.v1.1 MD05G1232400.v1.1 MD05G1233000.v1.1 MD10G1202800.v1.1
prunus_persica Prupe.4G139500_v2.0.a1 Prupe.4G139500_v2.0.a1
pyrus_communis pycom05g20170 pycom05g20200 pycom05g20230 pycom05g20250 pycom05g20260 pycom05g20280 pycom05g20320 pycom05g20370 pycom05g20380 pycom05g24850 pycom10g17340
rosa_chinensis RchiOBHm_Chr5g0026351 RchiOBHm_Chr5g0026371 RchiOBHm_Chr5g0026381 RchiOBHm_Chr5g0026411 RchiOBHm_Chr5g0026551 RchiOBHm_Chr5g0036371
rosa_laevigata RLG00000032923 RLG00000032926 RLG00000032927 RLG00000032928 RLG00000032934 RLG00000033692
rosa_multiflora Rmu_co8389779.1_g000001 Rmu_sc0000712.1_g000012 Rmu_sc0001017.1_g000026 Rmu_sc0001114.1_g000001 Rmu_sc0001114.1_g000002 Rmu_sc0001114.1_g000008 Rmu_sc0005581.1_g000006 Rmu_sc0005581.1_g000007 Rmu_sc0006059.1_g000052 Rmu_sc0042966.1_g000001
rosa_roxburghii Rroxscaffold_1G00044440 Rroxscaffold_1G00052930 Rroxscaffold_1G00052980 Rroxscaffold_1G00052990 Rroxscaffold_1G00053000
rosa_rugosa Rorug05G0092500 Rorug05G0092500 Rorug05G0092500 Rorug05G0092600 Rorug05G0092700 Rorug05G0092700 Rorug05G0093700 Rorug05G0155200 Rorug05G0155800 Rorug05G0155800
rosa_samantha Rh5AG185700 Rh5AG186300 Rh5BG182600 Rh5BG182900 Rh5BG183600 Rh5BG249400 Rh5CG202000 Rh5CG202100 Rh5CG202200 Rh5CG203400 Rh5CG281200 Rh5DG184400 Rh5DG184500 Rh5DG185900
rosa_wichuraiana Rw5G016850 Rw5G016870 Rw5G016930 Rw5G022870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1947
Acc36I ACCTGC 2 cut(s) 419, 2055
AccB7I CCANNNNNTGG 3 cut(s) 273, 602, 1325
AciI CCGC 1 cut(s) 888
AclWI GGATC 7 cut(s) 362, 494, 782, 1229, 1304, 1330, 2159
AcsI RAATTY 5 cut(s) 237, 914, 1016, 1114, 1799
AcuI CTGAAG 2 cut(s) 1019, 1260
AcyI GRCGYC 1 cut(s) 1944
AdeI CACNNNGTG 1 cut(s) 845
AfaI GTAC 3 cut(s) 31, 143, 1004
AfeI AGCGCT 2 cut(s) 764, 1688
AfiI CCNNNNNNNGG 7 cut(s) 273, 509, 602, 632, 792, 887, 1325
AflIII ACRYGT 1 cut(s) 1896
AgsI TTSAA 8 cut(s) 69, 466, 527, 898, 1113, 1279, 1516, 1586
AjnI CCWGG 6 cut(s) 232, 409, 728, 1037, 1338, 2242
AjuI GAANNNNNNNTTGG 2 cut(s) 775, 807
Alw21I GWGCWC 2 cut(s) 90, 1085
Alw26I GTCTC 1 cut(s) 50
Alw44I GTGCAC 1 cut(s) 1081
AlwI GGATC 7 cut(s) 362, 494, 782, 1229, 1304, 1330, 2159
AlwNI CAGNNNCTG 1 cut(s) 1092
Ama87I CYCGRG 2 cut(s) 536, 1713
Aor51HI AGCGCT 2 cut(s) 764, 1688
AoxI GGCC 3 cut(s) 1342, 1679, 2177
ApaLI GTGCAC 1 cut(s) 1081
ApeKI GCWGC 3 cut(s) 760, 891, 1220
ApoI RAATTY 5 cut(s) 237, 914, 1016, 1114, 1799
Asp700I GAANNNNTTC 2 cut(s) 555, 1093
AspLEI GCGC 5 cut(s) 113, 266, 765, 1689, 2130
AspS9I GGNCC 6 cut(s) 276, 533, 593, 635, 1342, 2067
AsuC2I CCSGG 2 cut(s) 537, 538
AsuHPI GGTGA 2 cut(s) 425, 2225
AsuNHI GCTAGC 1 cut(s) 424
AvaI CYCGRG 2 cut(s) 536, 1713
AvaII GGWCC 5 cut(s) 276, 533, 593, 635, 2067
BaeGI GKGCMC 1 cut(s) 1085
BaeI ACNNNNGTAYC 2 cut(s) 2076, 2109
BanII GRGCYC 2 cut(s) 90, 1722
BbsI GAAGAC 1 cut(s) 1248
Bbv12I GWGCWC 2 cut(s) 90, 1085
BbvI GCAGC 3 cut(s) 772, 878, 1232
BccI CCATC 9 cut(s) 50, 68, 344, 743, 811, 1069, 1210, 1327, 2119
BceAI ACGGC 1 cut(s) 67
BciT130I CCWGG 6 cut(s) 234, 411, 730, 1039, 1340, 2244
BclI TGATCA 3 cut(s) 673, 679, 1651
BcnI CCSGG 2 cut(s) 537, 538
BcoDI GTCTC 1 cut(s) 50
BfmI CTRYAG 4 cut(s) 105, 694, 758, 1857
BfoI RGCGCY 4 cut(s) 114, 267, 766, 1690
BfuAI ACCTGC 2 cut(s) 419, 2055
BglII AGATCT 2 cut(s) 1765, 1812
BisI GCNGC 4 cut(s) 761, 889, 892, 1221
BlsI GCNGC 4 cut(s) 762, 890, 893, 1222
BmcAI AGTACT 1 cut(s) 1004
Bme1390I CCNGG 8 cut(s) 234, 411, 537, 538, 730, 1039, 1340, 2244
Bme18I GGWCC 5 cut(s) 276, 533, 593, 635, 2067
BmeT110I CYCGRG 2 cut(s) 536, 1713
BmgT120I GGNCC 6 cut(s) 276, 533, 593, 635, 1342, 2067
BmiI GGNNCC 5 cut(s) 79, 535, 797, 1721, 1885
BmrFI CCNGG 8 cut(s) 234, 411, 537, 538, 730, 1039, 1340, 2244
BmrI ACTGGG 2 cut(s) 875, 1149
BmsI GCATC 3 cut(s) 481, 1232, 1590
BmtI GCTAGC 1 cut(s) 428
BmuI ACTGGG 2 cut(s) 875, 1149
BoxI GACNNNNGTC 2 cut(s) 1409, 1824
BpiI GAAGAC 1 cut(s) 1248
BplI GAGNNNNNCTC 2 cut(s) 952, 984
BpmI CTGGAG 1 cut(s) 1529
Bpu10I CCTNAGC 1 cut(s) 1683
BpuEI CTTGAG 3 cut(s) 492, 729, 1046
BpuMI CCSGG 2 cut(s) 537, 538
Bsa29I ATCGAT 2 cut(s) 124, 777
BsaAI YACGTR 1 cut(s) 130
BsaBI GATNNNNATC 2 cut(s) 678, 1604
BsaHI GRCGYC 1 cut(s) 1944
BsaI GGTCTC 1 cut(s) 50
BsaJI CCNNGG 7 cut(s) 232, 409, 536, 537, 596, 1339, 2243
BsaWI WCCGGW 1 cut(s) 609
Bsc4I CCNNNNNNNGG 7 cut(s) 273, 509, 602, 632, 792, 887, 1325
Bse1I ACTGG 4 cut(s) 595, 881, 1144, 1330
Bse3DI GCAATG 3 cut(s) 419, 988, 2222
Bse8I GATNNNNATC 2 cut(s) 678, 1604
BseBI CCWGG 6 cut(s) 234, 411, 730, 1039, 1340, 2244
BseCI ATCGAT 2 cut(s) 124, 777
BseDI CCNNGG 7 cut(s) 232, 409, 536, 537, 596, 1339, 2243
BseGI GGATG 3 cut(s) 1221, 1580, 2008
BseJI GATNNNNATC 2 cut(s) 678, 1604
BseLI CCNNNNNNNGG 7 cut(s) 273, 509, 602, 632, 792, 887, 1325
BseMI GCAATG 3 cut(s) 419, 988, 2222
BseMII CTCAG 6 cut(s) 232, 551, 732, 1739, 1830, 2106
BseNI ACTGG 4 cut(s) 595, 881, 1144, 1330
BseRI GAGGAG 1 cut(s) 1712
BseSI GKGCMC 1 cut(s) 1085
BseXI GCAGC 3 cut(s) 772, 878, 1232
BsgI GTGCAG 3 cut(s) 1084, 1378, 2177
BshFI GGCC 3 cut(s) 1344, 1681, 2179
BshVI ATCGAT 2 cut(s) 124, 777
BsiHKAI GWGCWC 2 cut(s) 90, 1085
BsiHKCI CYCGRG 2 cut(s) 536, 1713
BsiSI CCGG 2 cut(s) 537, 610
BslFI GGGAC 4 cut(s) 477, 519, 944, 1885
BslI CCNNNNNNNGG 7 cut(s) 273, 509, 602, 632, 792, 887, 1325
BsmAI GTCTC 1 cut(s) 50
BsmFI GGGAC 4 cut(s) 477, 519, 944, 1885
BsmI GAATGC 2 cut(s) 1581, 1619
BsnI GGCC 3 cut(s) 1344, 1681, 2179
Bso31I GGTCTC 1 cut(s) 50
BsoBI CYCGRG 2 cut(s) 536, 1713
Bsp1286I GDGCHC 3 cut(s) 90, 1085, 1722
Bsp19I CCATGG 1 cut(s) 596
BspACI CCGC 1 cut(s) 888
BspANI GGCC 3 cut(s) 1344, 1681, 2179
BspCNI CTCAG 6 cut(s) 231, 552, 731, 1740, 1829, 2107
BspDI ATCGAT 2 cut(s) 124, 777
BspHI TCATGA 2 cut(s) 676, 682
BspLI GGNNCC 5 cut(s) 79, 535, 797, 1721, 1885
BspMAI CTGCAG 2 cut(s) 109, 762
BspMI ACCTGC 2 cut(s) 419, 2055
BspOI GCTAGC 1 cut(s) 428
BspPI GGATC 7 cut(s) 362, 494, 782, 1229, 1304, 1330, 2159
BspTNI GGTCTC 1 cut(s) 50
BsrDI GCAATG 3 cut(s) 419, 988, 2222
BsrI ACTGG 4 cut(s) 595, 881, 1144, 1330
BssECI CCNNGG 7 cut(s) 232, 409, 536, 537, 596, 1339, 2243
BssNI GRCGYC 1 cut(s) 1944
BssT1I CCWWGG 1 cut(s) 596
Bst2UI CCWGG 6 cut(s) 234, 411, 730, 1039, 1340, 2244
Bst4CI ACNGT 8 cut(s) 166, 271, 454, 827, 1094, 1399, 1429, 1858
Bst6I CTCTTC 1 cut(s) 1982
BstACI GRCGYC 1 cut(s) 1944
BstAPI GCANNNNNTGC 1 cut(s) 857
BstBAI YACGTR 1 cut(s) 130
BstDEI CTNAG 7 cut(s) 218, 560, 718, 1683, 1748, 1816, 2115
BstDSI CCRYGG 1 cut(s) 596
BstENI CCTNNNNNAGG 2 cut(s) 507, 630
BstF5I GGATG 3 cut(s) 1221, 1580, 2008
BstH2I RGCGCY 4 cut(s) 114, 267, 766, 1690
BstHHI GCGC 5 cut(s) 113, 266, 765, 1689, 2130
BstMAI GTCTC 1 cut(s) 50
BstMWI GCNNNNNNNGC 3 cut(s) 760, 857, 2309
BstNI CCWGG 6 cut(s) 234, 411, 730, 1039, 1340, 2244
BstNSI RCATGY 6 cut(s) 1621, 1669, 1900, 2042, 2188, 2307
BstPAI GACNNNNGTC 2 cut(s) 1409, 1824
BstSCI CCNGG 8 cut(s) 232, 409, 535, 536, 728, 1037, 1338, 2242
BstSFI CTRYAG 4 cut(s) 105, 694, 758, 1857
BstSLI GKGCMC 1 cut(s) 1085
BstSNI TACGTA 1 cut(s) 130
BstV1I GCAGC 3 cut(s) 772, 878, 1232
BstV2I GAAGAC 1 cut(s) 1248
BstX2I RGATCY 4 cut(s) 1234, 1765, 1812, 2164
BstXI CCANNNNNNTGG 1 cut(s) 927
BstYI RGATCY 4 cut(s) 1234, 1765, 1812, 2164
Bsu15I ATCGAT 2 cut(s) 124, 777
BsuRI GGCC 3 cut(s) 1344, 1681, 2179
BsuTUI ATCGAT 2 cut(s) 124, 777
BtgI CCRYGG 1 cut(s) 596
BtsCI GGATG 3 cut(s) 1221, 1580, 2008
BtsI GCAGTG 3 cut(s) 315, 361, 1101
BtsIMutI CAGTG 5 cut(s) 198, 315, 361, 1101, 1185
BveI ACCTGC 2 cut(s) 419, 2055
CaiI CAGNNNCTG 1 cut(s) 1092
CciI TCATGA 2 cut(s) 676, 682
CfoI GCGC 5 cut(s) 113, 266, 765, 1689, 2130
Cfr13I GGNCC 6 cut(s) 276, 533, 593, 635, 1342, 2067
Cfr9I CCCGGG 1 cut(s) 536
ClaI ATCGAT 2 cut(s) 124, 777
Csp6I GTAC 3 cut(s) 30, 142, 1003
CspCI CAANNNNNGTGG 4 cut(s) 291, 326, 2121, 2156
CviQI GTAC 3 cut(s) 30, 142, 1003
DdeI CTNAG 7 cut(s) 218, 560, 718, 1683, 1748, 1816, 2115
DraIII CACNNNGTG 1 cut(s) 845
Eam1104I CTCTTC 1 cut(s) 1982
EarI CTCTTC 1 cut(s) 1982
Ecl136II GAGCTC 1 cut(s) 88
Eco105I TACGTA 1 cut(s) 130
Eco130I CCWWGG 1 cut(s) 596
Eco24I GRGCYC 2 cut(s) 90, 1722
Eco31I GGTCTC 1 cut(s) 50
Eco32I GATATC 1 cut(s) 153
Eco47I GGWCC 5 cut(s) 276, 533, 593, 635, 2067
Eco47III AGCGCT 2 cut(s) 764, 1688
Eco53kI GAGCTC 1 cut(s) 88
Eco57I CTGAAG 2 cut(s) 1019, 1260
Eco88I CYCGRG 2 cut(s) 536, 1713
EcoICRI GAGCTC 1 cut(s) 88
EcoNI CCTNNNNNAGG 2 cut(s) 507, 630
EcoRI GAATTC 1 cut(s) 237
EcoRII CCWGG 6 cut(s) 232, 409, 728, 1037, 1338, 2242
EcoRV GATATC 1 cut(s) 153
EcoT14I CCWWGG 1 cut(s) 596
EcoT22I ATGCAT 2 cut(s) 1619, 2044
EcoT38I GRGCYC 2 cut(s) 90, 1722
ErhI CCWWGG 1 cut(s) 596
FalI AAGNNNNNCTT 2 cut(s) 738, 770
FaqI GGGAC 4 cut(s) 477, 519, 944, 1885
FauNDI CATATG 2 cut(s) 137, 853
FbaI TGATCA 3 cut(s) 673, 679, 1651
Fnu4HI GCNGC 4 cut(s) 761, 889, 892, 1221
FokI GGATG 3 cut(s) 1228, 1587, 1995
FriOI GRGCYC 2 cut(s) 90, 1722
Fsp4HI GCNGC 4 cut(s) 761, 889, 892, 1221
GlaI GCGC 5 cut(s) 112, 265, 764, 1688, 2129
GluI GCNGC 4 cut(s) 761, 889, 892, 1221
GsuI CTGGAG 1 cut(s) 1529
HaeII RGCGCY 4 cut(s) 114, 267, 766, 1690
HaeIII GGCC 3 cut(s) 1344, 1681, 2179
HapII CCGG 2 cut(s) 537, 610
HhaI GCGC 5 cut(s) 113, 266, 765, 1689, 2130
Hin1I GRCGYC 1 cut(s) 1944
Hin6I GCGC 5 cut(s) 111, 264, 763, 1687, 2128
HinP1I GCGC 5 cut(s) 111, 264, 763, 1687, 2128
HincII GTYRAC 2 cut(s) 253, 741
HindII GTYRAC 2 cut(s) 253, 741
HindIII AAGCTT 4 cut(s) 437, 704, 1565, 1630
HpaI GTTAAC 1 cut(s) 741
HpaII CCGG 2 cut(s) 537, 610
HphI GGTGA 2 cut(s) 425, 2225
Hpy166II GTNNAC 5 cut(s) 253, 649, 741, 1083, 1265
Hpy8I GTNNAC 5 cut(s) 253, 649, 741, 1083, 1265
Hpy99I CGWCG 1 cut(s) 1946
HpyAV CCTTC 6 cut(s) 513, 1124, 1360, 2039, 2054, 2210
HpyCH4III ACNGT 8 cut(s) 166, 271, 454, 827, 1094, 1399, 1429, 1858
HpyCH4IV ACGT 2 cut(s) 129, 1944
HpyF10VI GCNNNNNNNGC 3 cut(s) 760, 857, 2309
HpyF3I CTNAG 7 cut(s) 218, 560, 718, 1683, 1748, 1816, 2115
HpySE526I ACGT 2 cut(s) 129, 1944
Hsp92I GRCGYC 1 cut(s) 1944
HspAI GCGC 5 cut(s) 111, 264, 763, 1687, 2128
Ksp22I TGATCA 3 cut(s) 673, 679, 1651
KspAI GTTAAC 1 cut(s) 741
LmnI GCTCC 1 cut(s) 1725
Lsp1109I GCAGC 3 cut(s) 772, 878, 1232
LweI GCATC 3 cut(s) 481, 1232, 1590
MaeII ACGT 2 cut(s) 129, 1944
MaeIII GTNAC 7 cut(s) 157, 356, 454, 827, 1525, 1892, 1916
MfeI CAATTG 1 cut(s) 466
MflI RGATCY 4 cut(s) 1234, 1765, 1812, 2164
MhlI GDGCHC 3 cut(s) 90, 1085, 1722
MlyI GAGTC 1 cut(s) 961
MmeI TCCRAC 5 cut(s) 306, 1411, 1861, 1950, 1965
Mph1103I ATGCAT 2 cut(s) 1619, 2044
MroXI GAANNNNTTC 2 cut(s) 555, 1093
MseI TTAA 6 cut(s) 740, 806, 1044, 1304, 1419, 1803
MslI CAYNNNNRTG 2 cut(s) 681, 1214
MspI CCGG 2 cut(s) 537, 610
MspR9I CCNGG 8 cut(s) 234, 411, 537, 538, 730, 1039, 1340, 2244
MunI CAATTG 1 cut(s) 466
Mva1269I GAATGC 2 cut(s) 1581, 1619
MvaI CCWGG 6 cut(s) 234, 411, 730, 1039, 1340, 2244
MwoI GCNNNNNNNGC 3 cut(s) 760, 857, 2309
NciI CCSGG 2 cut(s) 537, 538
NcoI CCATGG 1 cut(s) 596
NdeI CATATG 2 cut(s) 137, 853
NheI GCTAGC 1 cut(s) 424
NlaIV GGNNCC 5 cut(s) 79, 535, 797, 1721, 1885
NmeAIII GCCGAG 1 cut(s) 2288
NmuCI GTSAC 3 cut(s) 356, 827, 1916
NsiI ATGCAT 2 cut(s) 1619, 2044
NspI RCATGY 6 cut(s) 1621, 1669, 1900, 2042, 2188, 2307
PaeI GCATGC 3 cut(s) 1621, 1669, 2307
PagI TCATGA 2 cut(s) 676, 682
PciI ACATGT 1 cut(s) 1896
PctI GAATGC 2 cut(s) 1581, 1619
PdmI GAANNNNTTC 2 cut(s) 555, 1093
PfeI GAWTC 9 cut(s) 93, 203, 222, 326, 606, 1555, 1739, 2001, 2047
PflMI CCANNNNNTGG 3 cut(s) 273, 602, 1325
PkrI GCNGC 4 cut(s) 762, 890, 893, 1222
PleI GAGTC 1 cut(s) 961
PpsI GAGTC 1 cut(s) 961
Ppu21I YACGTR 1 cut(s) 130
PscI ACATGT 1 cut(s) 1896
PshAI GACNNNNGTC 2 cut(s) 1409, 1824
Psp124BI GAGCTC 1 cut(s) 90
Psp6I CCWGG 6 cut(s) 232, 409, 728, 1037, 1338, 2242
PspGI CCWGG 6 cut(s) 232, 409, 728, 1037, 1338, 2242
PspN4I GGNNCC 5 cut(s) 79, 535, 797, 1721, 1885
PspPI GGNCC 6 cut(s) 276, 533, 593, 635, 1342, 2067
PstI CTGCAG 2 cut(s) 109, 762
PstNI CAGNNNCTG 1 cut(s) 1092
PsuI RGATCY 4 cut(s) 1234, 1765, 1812, 2164
RsaI GTAC 3 cut(s) 31, 143, 1004
RsaNI GTAC 3 cut(s) 30, 142, 1003
RseI CAYNNNNRTG 2 cut(s) 681, 1214
SacI GAGCTC 1 cut(s) 90
SaqAI TTAA 6 cut(s) 740, 806, 1044, 1304, 1419, 1803
SatI GCNGC 4 cut(s) 761, 889, 892, 1221
Sau96I GGNCC 6 cut(s) 276, 533, 593, 635, 1342, 2067
SbfI CCTGCAGG 1 cut(s) 109
ScaI AGTACT 1 cut(s) 1004
SchI GAGTC 1 cut(s) 961
ScrFI CCNGG 8 cut(s) 234, 411, 537, 538, 730, 1039, 1340, 2244
SdaI CCTGCAGG 1 cut(s) 109
SduI GDGCHC 3 cut(s) 90, 1085, 1722
SfaNI GCATC 3 cut(s) 481, 1232, 1590
SfcI CTRYAG 4 cut(s) 105, 694, 758, 1857
SinI GGWCC 5 cut(s) 276, 533, 593, 635, 2067
SmaI CCCGGG 1 cut(s) 538
SmiMI CAYNNNNRTG 2 cut(s) 681, 1214
SmlI CTYRAG 4 cut(s) 507, 744, 1061, 2336
SmoI CTYRAG 4 cut(s) 507, 744, 1061, 2336
SnaBI TACGTA 1 cut(s) 130
SphI GCATGC 3 cut(s) 1621, 1669, 2307
Sse8387I CCTGCAGG 1 cut(s) 109
SsiI CCGC 1 cut(s) 888
SspI AATATT 2 cut(s) 868, 1783
SstI GAGCTC 1 cut(s) 90
StyD4I CCNGG 8 cut(s) 232, 409, 535, 536, 728, 1037, 1338, 2242
StyI CCWWGG 1 cut(s) 596
TaaI ACNGT 8 cut(s) 166, 271, 454, 827, 1094, 1399, 1429, 1858
TaiI ACGT 2 cut(s) 132, 1947
TaqI TCGA 2 cut(s) 124, 777
TatI WGTACW 2 cut(s) 29, 1002
TauI GCSGC 1 cut(s) 891
TfiI GAWTC 9 cut(s) 93, 203, 222, 326, 606, 1555, 1739, 2001, 2047
Tru1I TTAA 6 cut(s) 740, 806, 1044, 1304, 1419, 1803
Tru9I TTAA 6 cut(s) 740, 806, 1044, 1304, 1419, 1803
TscAI CASTG 5 cut(s) 205, 315, 361, 1108, 1192
TseFI GTSAC 3 cut(s) 356, 827, 1916
TseI GCWGC 3 cut(s) 760, 891, 1220
Tsp45I GTSAC 3 cut(s) 356, 827, 1916
TspGWI ACGGA 1 cut(s) 2072
TspMI CCCGGG 1 cut(s) 536
TspRI CASTG 5 cut(s) 205, 315, 361, 1108, 1192
Van91I CCANNNNNTGG 3 cut(s) 273, 602, 1325
VneI GTGCAC 1 cut(s) 1081
VpaK11BI GGWCC 5 cut(s) 276, 533, 593, 635, 2067
XagI CCTNNNNNAGG 2 cut(s) 507, 630
XapI RAATTY 5 cut(s) 237, 914, 1016, 1114, 1799
XbaI TCTAGA 2 cut(s) 1742, 2297
XceI RCATGY 6 cut(s) 1621, 1669, 1900, 2042, 2188, 2307
XmaI CCCGGG 1 cut(s) 536
XmnI GAANNNNTTC 2 cut(s) 555, 1093
ZraI GACGTC 1 cut(s) 1945
ZrmI AGTACT 1 cut(s) 1004
Zsp2I ATGCAT 2 cut(s) 1619, 2044
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.