Rh5BG182600

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0000484
Physical Location & Seq
Forward (+)
20041955 .. 20045704
3750 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG182600.1

Sequence Viewer

Length: 2577 bp
ATGGAAATGAGGTGTTCGAGTATTACAGTCATGAAAAATCCATTGAAGGTTTTAATTAGGATGTGCTTTCATCCTATTTGCTCTTTTCTGCTCATCATTACAGCGATATTTTCCACAGTGGGAGGCAGCACAAGTACATTTCAGTCCGTTAGAGATGGCCAGACTATAGTTTCAACTACTGGAAACTTTGAGCTTGGATTTTTTAGTCCTGCTACTGGTTCTAATAACCGATACCTTGGGATATGGTACAAGAAGATATCGGCTACCATAGTATGGGTTGCCAACAGAGAAGCACCCCTTATTGATTCATCAGGTGTTCTGCAGGTCACCGACCCTGGAATTCTTGTCCTCCTCAACCATAACAAGAGCATAGTTTGGTCCTCAAACACATCAAGATCTGCAGAGAATCCAGTGGCCCAGCTTTTGGATTCAGGAAATCTTGTTGTAACAGACGGCAGTGATACAGACCCTGAGAACTTCTTGTGGCAAAGTTTTGATTACCCAAGTGATACACTCCTACCAGGTATGAAGCTTGGTTGGAACAAAGTTACTGGCTTCAGTAGGCATCTTACATCATGGAACAGTCCACAGGATCCTTCTCTAGGAATATATACATATGAACTTGATCGCAGAGGATATGGAGAAGAAATTTTGAAGAAGGATTCAGTTATGATATGGCGAACTGGACCATGGAATGGAATCCGATTCAGTGGCACGCCTCATTTAAGTCCAAACTCCATATTCACATACAATCTTGTTTTTGATGATCATGATGAAGAATATTATATTTTTGAGCTTCTTAACAGCTCAGTTTATTCAAGACTGATCCTCACTCCAGATGGGCGTGTGCAGCGCTACACATGGATTGATACAACCCAAACTTGGGGATTTCTTTATCAAACAGCTTTAATTGATAACTGCGACAATTATGCATTCTGTGGTCCAAATGGTGCATGCAGTATTGACAGTTCCCCAGTATGTCGCTGCTTGAATGGATTTCAACCCAAAAATCCAAAGGACTGGGATGTAGTGGATTGGTCAAATGGCTGTGTGAGAAGGACTCCACTAAATTCAGTAAATTGCAGCGGTGACTTGTTTGAGAAGTACTCTGCAGTGAAATTGCCAAACACAGAGCAGTCCTGGTTCAACAGAAGTATGAATCTGAAGGAATGTGAGATGGTGTGCCTGAAGAACTGTTCCTGCACAGCTTATGCAAATTTGGATATCACGGATGGAGGATCTGGCTGCTTGCTGTGGTTTGGCAACCTAATTGATATTAGAAATTTAACTGTAAATGGGCAAGATATTTATATAAGAATGGCTGCATCAGAACTAGGCCTGGATCATGACAACACAGTTATCAATGCTAAAGACTCTGAATCTAAGAAGGTTAGAATCATACTATGTGGTACTCTGTTGCCTACTGGACTGCTGATCGTGGGCATAGCCCTGGTATTTTATTGCCGGAAGAAGCAGCGCAAAAAATATGGGAAAATGAGGAGCAGCAAAAAAGAAGATTCGGAACTTACCTCAAGAGAAGAAGATTTAGGACTTCCCTCAAAGAAGGAAGATTTTGAACTTCCATTATTTGACTTGGCTACTGTCGTTTGTGCCACCGATAACTTCTCAGAGAGCAGCAAACTTGGAGAAGGTGGATTCGGATCCGTCTTTAAGGGTATGTTGAAAGATGGACAGGAAATAGCTGTGAAGAGGCTCTCGAAACATTCTACTCAAGGACTGGATGAGTTCAAGAATGAGGTTACACATATTGTCAAACTTCAGCACAGGAATCTAGTGAGGCTTCTAGGATGCTGCATTCAGGAAGATGAAATGATGCTGATCTACGAATGCATGCCTAACAAAAGCTTGGACTTCTTTATTTTTGATCAAAGAAGAAGCTTCTTACTAGATTGGTCTAAGTGCTTTGACATTATTAATGGTATTGCTCGGGGGATCCTTTATCTTCATCAAGATTCTAGACTGAGGGTAATTCATAGAGATCTCAAAGCAGGCAATATCTTATTAGACAGTGAATTCAACCCAAAAATCTCAGACTTCGGCCTGGCTAGAAGTTTTGGAGGAAATGAAACTAAAGCAGAAACGAATAAAGTGGTCGGGACATATGGTTACATGTCCCCAGAATATGCAATTGACGGGTTCTACTCGATCAAATCTGACGTGTATAGCTTTGGTGTTTTGATGCTGGAGATAATAAGTGGGAAGAGAAACAGAGGATTCCTTCATCCAGGCCACAACCTCAACCTGCTTGGACATGCATGGAACTTACACACAGAAGGCAGGTCCATTGAATTGCTTGCTACATCAGTAGGGGACTCCATTAATCTACAAGAAGTTCTGCGAGCGATTCATGTGGGTCTTTTATGTGTTCAGCAAAATCCACAAGACAGGCCAAGCATGTCAGCTGCAGTTCTAATGTTAAGTGGTGAAGGTGTGCTGCCTGTACCTCAGAAACCTGGTTTTTATAATGAGAGGGCTCTAACTGAACTCCAAGTCCATTCTTCTTCACAAGCATGTTCAGTTAATATGCTCACTTCCACCCTATTCGAGCCTCGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

858

Amino Acids

96.11

Weight (kDa)

6.07

Isoelectric Point (pI)

44.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 89 - 193 4e-40 D-mannose binding lectin
S_locus_glycop PF00954 226 - 337 1.2e-29 S-locus glycoprotein domain
PAN_2 PF08276 361 - 426 9.5e-21 PAN-like domain
Pkinase PF00069 543 - 742 1.5e-44 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 545 - 808 1.6e-46 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000484)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27290 AT4G27290
fragaria_vesca FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g21310 FvH4_3g21310
malus_domestica MD05G1219900.v1.1 MD05G1220200.v1.1 MD05G1230800.v1.1 MD05G1231100.v1.1 MD05G1231700.v1.1 MD05G1232100.v1.1 MD05G1232400.v1.1 MD05G1233000.v1.1 MD10G1202800.v1.1
prunus_persica Prupe.4G139500_v2.0.a1 Prupe.4G139500_v2.0.a1
pyrus_communis pycom05g20170 pycom05g20200 pycom05g20230 pycom05g20250 pycom05g20260 pycom05g20280 pycom05g20320 pycom05g20370 pycom05g20380 pycom05g24850 pycom10g17340
rosa_chinensis RchiOBHm_Chr5g0026351 RchiOBHm_Chr5g0026371 RchiOBHm_Chr5g0026381 RchiOBHm_Chr5g0026411 RchiOBHm_Chr5g0026551 RchiOBHm_Chr5g0036371
rosa_laevigata RLG00000032923 RLG00000032926 RLG00000032927 RLG00000032928 RLG00000032934 RLG00000033692
rosa_multiflora Rmu_co8389779.1_g000001 Rmu_sc0000712.1_g000012 Rmu_sc0001017.1_g000026 Rmu_sc0001114.1_g000001 Rmu_sc0001114.1_g000002 Rmu_sc0001114.1_g000008 Rmu_sc0005581.1_g000006 Rmu_sc0005581.1_g000007 Rmu_sc0006059.1_g000052 Rmu_sc0042966.1_g000001
rosa_roxburghii Rroxscaffold_1G00044440 Rroxscaffold_1G00052930 Rroxscaffold_1G00052980 Rroxscaffold_1G00052990 Rroxscaffold_1G00053000
rosa_rugosa Rorug05G0092500 Rorug05G0092500 Rorug05G0092500 Rorug05G0092600 Rorug05G0092700 Rorug05G0092700 Rorug05G0093700 Rorug05G0155200 Rorug05G0155800 Rorug05G0155800
rosa_samantha Rh5AG185700 Rh5AG186300 Rh5BG182600 Rh5BG182900 Rh5BG183600 Rh5BG249400 Rh5CG202000 Rh5CG202100 Rh5CG202200 Rh5CG203400 Rh5CG281200 Rh5DG184400 Rh5DG184500 Rh5DG185900
rosa_wichuraiana Rw5G016850 Rw5G016870 Rw5G016930 Rw5G022870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2484
Acc36I ACCTGC 3 cut(s) 313, 2271, 2289
AccB7I CCANNNNNTGG 3 cut(s) 273, 424, 695
AciI CCGC 1 cut(s) 1086
AclWI GGATC 9 cut(s) 587, 600, 820, 1246, 1350, 1656, 1669, 1948, 1961
AcoI YGGCCR 1 cut(s) 157
AcsI RAATTY 6 cut(s) 339, 648, 1069, 1216, 1282, 2033
AcuI CTGAAG 4 cut(s) 541, 1184, 1208, 1763
AfaI GTAC 5 cut(s) 136, 248, 1106, 1411, 2463
AfeI AGCGCT 1 cut(s) 854
AfiI CCNNNNNNNGG 7 cut(s) 215, 273, 424, 602, 695, 882, 883
AflIII ACRYGT 2 cut(s) 2130, 2178
AjiI CACGTC 1 cut(s) 2179
AjnI CCWGG 8 cut(s) 334, 520, 1139, 1338, 1449, 2061, 2245, 2473
AloI GAACNNNNNNTCC 2 cut(s) 2496, 2528
AlwI GGATC 9 cut(s) 587, 600, 820, 1246, 1350, 1656, 1669, 1948, 1961
AlwNI CAGNNNCTG 1 cut(s) 470
Ama87I CYCGRG 1 cut(s) 1947
Aor51HI AGCGCT 1 cut(s) 854
AoxI GGCC 6 cut(s) 157, 414, 1336, 2059, 2248, 2408
ApoI RAATTY 6 cut(s) 339, 648, 1069, 1216, 1282, 2033
AseI ATTAAT 2 cut(s) 1935, 2340
AspLEI GCGC 2 cut(s) 855, 1479
AspS9I GGNCC 5 cut(s) 378, 415, 686, 941, 2301
AsuHPI GGTGA 3 cut(s) 319, 1100, 2456
AvaI CYCGRG 1 cut(s) 1947
AvaII GGWCC 4 cut(s) 378, 686, 941, 2301
BalI TGGCCA 1 cut(s) 159
BamHI GGATCC 3 cut(s) 592, 1661, 1953
BanII GRGCYC 1 cut(s) 2497
BccI CCATC 5 cut(s) 149, 833, 1171, 1226, 1682
BceAI ACGGC 1 cut(s) 469
BcgI CGANNNNNNTGC 2 cut(s) 911, 945
BciT130I CCWGG 8 cut(s) 336, 522, 1141, 1340, 1451, 2063, 2247, 2475
BclI TGATCA 2 cut(s) 766, 1885
BfaI CTAG 7 cut(s) 602, 1334, 1793, 1805, 1907, 1977, 2067
BfmI CTRYAG 5 cut(s) 165, 320, 399, 1110, 2424
BfoI RGCGCY 1 cut(s) 856
BfuAI ACCTGC 3 cut(s) 313, 2271, 2289
BglII AGATCT 2 cut(s) 395, 1999
BmcAI AGTACT 1 cut(s) 1106
Bme1390I CCNGG 8 cut(s) 336, 522, 1141, 1340, 1451, 2063, 2247, 2475
Bme18I GGWCC 4 cut(s) 378, 686, 941, 2301
BmeT110I CYCGRG 1 cut(s) 1947
BmgBI CACGTC 1 cut(s) 2179
BmgT120I GGNCC 5 cut(s) 378, 415, 686, 941, 2301
BmiI GGNNCC 3 cut(s) 594, 1663, 1955
BmrFI CCNGG 8 cut(s) 336, 522, 1141, 1340, 1451, 2063, 2247, 2475
BmrI ACTGGG 2 cut(s) 968, 1030
BmsI GCATC 5 cut(s) 574, 1334, 1799, 1824, 2190
BmuI ACTGGG 2 cut(s) 968, 1030
BplI GAGNNNNNCTC 4 cut(s) 1045, 1077, 1091, 1123
BpmI CTGGAG 2 cut(s) 819, 2225
BpuEI CTTGAG 2 cut(s) 1516, 1716
BsaBI GATNNNNATC 2 cut(s) 1660, 1838
BsaJI CCNNGG 4 cut(s) 235, 334, 689, 1449
Bsc4I CCNNNNNNNGG 7 cut(s) 215, 273, 424, 602, 695, 882, 883
Bse1I ACTGG 9 cut(s) 184, 220, 410, 556, 688, 974, 1025, 1429, 1743
Bse8I GATNNNNATC 2 cut(s) 1660, 1838
BseBI CCWGG 8 cut(s) 336, 522, 1141, 1340, 1451, 2063, 2247, 2475
BseDI CCNNGG 4 cut(s) 235, 334, 689, 1449
BseGI GGATG 7 cut(s) 66, 70, 1030, 1237, 1747, 1814, 2242
BseJI GATNNNNATC 2 cut(s) 1660, 1838
BseLI CCNNNNNNNGG 7 cut(s) 215, 273, 424, 602, 695, 882, 883
BseMII CTCAG 6 cut(s) 462, 822, 1641, 1973, 2064, 2480
BseNI ACTGG 9 cut(s) 184, 220, 410, 556, 688, 974, 1025, 1429, 1743
BseRI GAGGAG 2 cut(s) 341, 1513
BseYI CCCAGC 1 cut(s) 417
BsgI GTGCAG 2 cut(s) 869, 1186
BshFI GGCC 6 cut(s) 159, 416, 1338, 2061, 2250, 2410
BsiHKCI CYCGRG 1 cut(s) 1947
BsiSI CCGG 1 cut(s) 1465
BslFI GGGAC 3 cut(s) 2119, 2131, 2345
BslI CCNNNNNNNGG 7 cut(s) 215, 273, 424, 602, 695, 882, 883
BsmFI GGGAC 3 cut(s) 2119, 2131, 2345
BsmI GAATGC 3 cut(s) 932, 1815, 1853
BsnI GGCC 6 cut(s) 159, 416, 1338, 2061, 2250, 2410
BsoBI CYCGRG 1 cut(s) 1947
Bsp1286I GDGCHC 1 cut(s) 2497
Bsp19I CCATGG 1 cut(s) 689
BspACI CCGC 1 cut(s) 1086
BspANI GGCC 6 cut(s) 159, 416, 1338, 2061, 2250, 2410
BspCNI CTCAG 6 cut(s) 463, 821, 1640, 1974, 2063, 2479
BspHI TCATGA 3 cut(s) 30, 769, 1345
BspLI GGNNCC 3 cut(s) 594, 1663, 1955
BspMAI CTGCAG 4 cut(s) 324, 403, 1114, 2428
BspMI ACCTGC 3 cut(s) 313, 2271, 2289
BspPI GGATC 9 cut(s) 587, 600, 820, 1246, 1350, 1656, 1669, 1948, 1961
BsrI ACTGG 9 cut(s) 184, 220, 410, 556, 688, 974, 1025, 1429, 1743
BssECI CCNNGG 4 cut(s) 235, 334, 689, 1449
BssT1I CCWWGG 2 cut(s) 235, 689
Bst2UI CCWGG 8 cut(s) 336, 522, 1141, 1340, 1451, 2063, 2247, 2475
Bst4CI ACNGT 9 cut(s) 28, 118, 584, 968, 1196, 1291, 1357, 1603, 2030
Bst6I CTCTTC 2 cut(s) 1703, 2216
BstC8I GCNNGC 7 cut(s) 716, 955, 1250, 1853, 2011, 2316, 2361
BstDEI CTNAG 8 cut(s) 471, 808, 1383, 1627, 1917, 1982, 2050, 2466
BstDSI CCRYGG 1 cut(s) 689
BstEII GGTNACC 1 cut(s) 325
BstENI CCTNNNNNAGG 1 cut(s) 600
BstF5I GGATG 7 cut(s) 66, 70, 1030, 1237, 1747, 1814, 2242
BstH2I RGCGCY 1 cut(s) 856
BstHHI GCGC 2 cut(s) 855, 1479
BstMWI GCNNNNNNNGC 1 cut(s) 850
BstNI CCWGG 8 cut(s) 336, 522, 1141, 1340, 1451, 2063, 2247, 2475
BstNSI RCATGY 6 cut(s) 957, 1855, 2134, 2276, 2419, 2535
BstPI GGTNACC 1 cut(s) 325
BstSCI CCNGG 8 cut(s) 334, 520, 1139, 1338, 1449, 2061, 2245, 2473
BstSFI CTRYAG 5 cut(s) 165, 320, 399, 1110, 2424
BstX2I RGATCY 6 cut(s) 395, 592, 1238, 1661, 1953, 1999
BstXI CCANNNNNNTGG 1 cut(s) 1020
BstYI RGATCY 6 cut(s) 395, 592, 1238, 1661, 1953, 1999
BsuRI GGCC 6 cut(s) 159, 416, 1338, 2061, 2250, 2410
BtgI CCRYGG 1 cut(s) 689
BtrI CACGTC 1 cut(s) 2179
BtsCI GGATG 7 cut(s) 66, 70, 1030, 1237, 1747, 1814, 2242
BtsI GCAGTG 2 cut(s) 463, 1119
BtsIMutI CAGTG 6 cut(s) 123, 417, 463, 715, 1119, 2035
BveI ACCTGC 3 cut(s) 313, 2271, 2289
Cac8I GCNNGC 7 cut(s) 716, 955, 1250, 1853, 2011, 2316, 2361
CaiI CAGNNNCTG 1 cut(s) 470
CciI TCATGA 3 cut(s) 30, 769, 1345
CfoI GCGC 2 cut(s) 855, 1479
Cfr13I GGNCC 5 cut(s) 378, 415, 686, 941, 2301
CsiI ACCWGGT 2 cut(s) 520, 2473
Csp6I GTAC 5 cut(s) 135, 247, 1105, 1410, 2462
CviQI GTAC 5 cut(s) 135, 247, 1105, 1410, 2462
DdeI CTNAG 8 cut(s) 471, 808, 1383, 1627, 1917, 1982, 2050, 2466
EaeI YGGCCR 1 cut(s) 157
Eam1104I CTCTTC 2 cut(s) 1703, 2216
EarI CTCTTC 2 cut(s) 1703, 2216
Eco130I CCWWGG 2 cut(s) 235, 689
Eco147I AGGCCT 1 cut(s) 1338
Eco24I GRGCYC 1 cut(s) 2497
Eco32I GATATC 2 cut(s) 258, 1225
Eco47I GGWCC 4 cut(s) 378, 686, 941, 2301
Eco47III AGCGCT 1 cut(s) 854
Eco57I CTGAAG 4 cut(s) 541, 1184, 1208, 1763
Eco88I CYCGRG 1 cut(s) 1947
Eco91I GGTNACC 1 cut(s) 325
EcoNI CCTNNNNNAGG 1 cut(s) 600
EcoO65I GGTNACC 1 cut(s) 325
EcoRI GAATTC 2 cut(s) 339, 2033
EcoRII CCWGG 8 cut(s) 334, 520, 1139, 1338, 1449, 2061, 2245, 2473
EcoRV GATATC 2 cut(s) 258, 1225
EcoT14I CCWWGG 2 cut(s) 235, 689
EcoT22I ATGCAT 3 cut(s) 934, 1853, 2278
EcoT38I GRGCYC 1 cut(s) 2497
ErhI CCWWGG 2 cut(s) 235, 689
FalI AAGNNNNNCTT 2 cut(s) 282, 314
FaqI GGGAC 3 cut(s) 2119, 2131, 2345
FauNDI CATATG 2 cut(s) 616, 2122
FbaI TGATCA 2 cut(s) 766, 1885
FokI GGATG 7 cut(s) 57, 73, 1037, 1244, 1754, 1821, 2229
FriOI GRGCYC 1 cut(s) 2497
FspBI CTAG 7 cut(s) 602, 1334, 1793, 1805, 1907, 1977, 2067
GlaI GCGC 2 cut(s) 854, 1478
GsaI CCCAGC 1 cut(s) 421
GsuI CTGGAG 2 cut(s) 819, 2225
HaeII RGCGCY 1 cut(s) 856
HaeIII GGCC 6 cut(s) 159, 416, 1338, 2061, 2250, 2410
HapII CCGG 1 cut(s) 1465
HhaI GCGC 2 cut(s) 855, 1479
Hin6I GCGC 2 cut(s) 853, 1477
HinP1I GCGC 2 cut(s) 853, 1477
HindIII AAGCTT 3 cut(s) 530, 1864, 1897
HpaII CCGG 1 cut(s) 1465
HphI GGTGA 3 cut(s) 319, 1100, 2456
Hpy166II GTNNAC 1 cut(s) 587
Hpy8I GTNNAC 1 cut(s) 587
HpyCH4III ACNGT 9 cut(s) 28, 118, 584, 968, 1196, 1291, 1357, 1603, 2030
HpyCH4IV ACGT 1 cut(s) 2178
HpyF10VI GCNNNNNNNGC 1 cut(s) 850
HpyF3I CTNAG 8 cut(s) 471, 808, 1383, 1627, 1917, 1982, 2050, 2466
HpySE526I ACGT 1 cut(s) 2178
HspAI GCGC 2 cut(s) 853, 1477
Ksp22I TGATCA 2 cut(s) 766, 1885
LmnI GCTCC 1 cut(s) 1500
LweI GCATC 5 cut(s) 574, 1334, 1799, 1824, 2190
MabI ACCWGGT 2 cut(s) 520, 2473
MaeI CTAG 7 cut(s) 602, 1334, 1793, 1805, 1907, 1977, 2067
MaeII ACGT 1 cut(s) 2178
MaeIII GTNAC 6 cut(s) 325, 445, 547, 1088, 1759, 2126
MfeI CAATTG 1 cut(s) 2148
MflI RGATCY 6 cut(s) 395, 592, 1238, 1661, 1953, 1999
MhlI GDGCHC 1 cut(s) 2497
MlsI TGGCCA 1 cut(s) 159
MluNI TGGCCA 1 cut(s) 159
MlyI GAGTC 3 cut(s) 1054, 1367, 2327
MmeI TCCRAC 1 cut(s) 518
Mox20I TGGCCA 1 cut(s) 159
Mph1103I ATGCAT 3 cut(s) 934, 1853, 2278
MscI TGGCCA 1 cut(s) 159
MslI CAYNNNNRTG 1 cut(s) 2530
Msp20I TGGCCA 1 cut(s) 159
MspA1I CMGCKG 2 cut(s) 1086, 2423
MspI CCGG 1 cut(s) 1465
MspR9I CCNGG 8 cut(s) 336, 522, 1141, 1340, 1451, 2063, 2247, 2475
MunI CAATTG 1 cut(s) 2148
Mva1269I GAATGC 3 cut(s) 932, 1815, 1853
MvaI CCWGG 8 cut(s) 336, 522, 1141, 1340, 1451, 2063, 2247, 2475
MwoI GCNNNNNNNGC 1 cut(s) 850
NcoI CCATGG 1 cut(s) 689
NdeI CATATG 2 cut(s) 616, 2122
NlaIV GGNNCC 3 cut(s) 594, 1663, 1955
NmuCI GTSAC 2 cut(s) 325, 1088
NsiI ATGCAT 3 cut(s) 934, 1853, 2278
NspI RCATGY 6 cut(s) 957, 1855, 2134, 2276, 2419, 2535
PaeI GCATGC 2 cut(s) 957, 1855
PagI TCATGA 3 cut(s) 30, 769, 1345
PceI AGGCCT 1 cut(s) 1338
PciI ACATGT 1 cut(s) 2130
PctI GAATGC 3 cut(s) 932, 1815, 1853
PflMI CCANNNNNTGG 3 cut(s) 273, 424, 695
PleI GAGTC 3 cut(s) 1054, 1367, 2327
PpsI GAGTC 3 cut(s) 1054, 1367, 2327
PscI ACATGT 1 cut(s) 2130
PshBI ATTAAT 2 cut(s) 1935, 2340
PsiI TTATAA 1 cut(s) 2484
Psp6I CCWGG 8 cut(s) 334, 520, 1139, 1338, 1449, 2061, 2245, 2473
PspEI GGTNACC 1 cut(s) 325
PspFI CCCAGC 1 cut(s) 417
PspGI CCWGG 8 cut(s) 334, 520, 1139, 1338, 1449, 2061, 2245, 2473
PspN4I GGNNCC 3 cut(s) 594, 1663, 1955
PspPI GGNCC 5 cut(s) 378, 415, 686, 941, 2301
PsrI GAACNNNNNNTAC 2 cut(s) 952, 984
PstI CTGCAG 4 cut(s) 324, 403, 1114, 2428
PstNI CAGNNNCTG 1 cut(s) 470
PsuI RGATCY 6 cut(s) 395, 592, 1238, 1661, 1953, 1999
PvuII CAGCTG 1 cut(s) 2423
RsaI GTAC 5 cut(s) 136, 248, 1106, 1411, 2463
RsaNI GTAC 5 cut(s) 135, 247, 1105, 1410, 2462
RseI CAYNNNNRTG 1 cut(s) 2530
Sau96I GGNCC 5 cut(s) 378, 415, 686, 941, 2301
ScaI AGTACT 1 cut(s) 1106
SchI GAGTC 3 cut(s) 1054, 1367, 2327
ScrFI CCNGG 8 cut(s) 336, 522, 1141, 1340, 1451, 2063, 2247, 2475
SduI GDGCHC 1 cut(s) 2497
SexAI ACCWGGT 2 cut(s) 520, 2473
SfaNI GCATC 5 cut(s) 574, 1334, 1799, 1824, 2190
SfcI CTRYAG 5 cut(s) 165, 320, 399, 1110, 2424
SinI GGWCC 4 cut(s) 378, 686, 941, 2301
SmiMI CAYNNNNRTG 1 cut(s) 2530
SmlI CTYRAG 2 cut(s) 1531, 1731
SmoI CTYRAG 2 cut(s) 1531, 1731
SphI GCATGC 2 cut(s) 957, 1855
SseBI AGGCCT 1 cut(s) 1338
SsiI CCGC 1 cut(s) 1086
SspI AATATT 1 cut(s) 782
SspMI CTAG 7 cut(s) 602, 1334, 1793, 1805, 1907, 1977, 2067
StuI AGGCCT 1 cut(s) 1338
StyD4I CCNGG 8 cut(s) 334, 520, 1139, 1338, 1449, 2061, 2245, 2473
StyI CCWWGG 2 cut(s) 235, 689
TaaI ACNGT 9 cut(s) 28, 118, 584, 968, 1196, 1291, 1357, 1603, 2030
TaiI ACGT 1 cut(s) 2181
TaqI TCGA 4 cut(s) 17, 1718, 2165, 2565
TatI WGTACW 2 cut(s) 134, 1104
TscAI CASTG 6 cut(s) 123, 417, 463, 715, 1119, 2035
TseFI GTSAC 2 cut(s) 325, 1088
Tsp45I GTSAC 2 cut(s) 325, 1088
TspGWI ACGGA 3 cut(s) 136, 1244, 1654
TspRI CASTG 6 cut(s) 123, 417, 463, 715, 1119, 2035
Van91I CCANNNNNTGG 3 cut(s) 273, 424, 695
VpaK11BI GGWCC 4 cut(s) 378, 686, 941, 2301
VspI ATTAAT 2 cut(s) 1935, 2340
XagI CCTNNNNNAGG 1 cut(s) 600
XapI RAATTY 6 cut(s) 339, 648, 1069, 1216, 1282, 2033
XbaI TCTAGA 1 cut(s) 1976
XceI RCATGY 6 cut(s) 957, 1855, 2134, 2276, 2419, 2535
XspI CTAG 7 cut(s) 602, 1334, 1793, 1805, 1907, 1977, 2067
ZrmI AGTACT 1 cut(s) 1106
Zsp2I ATGCAT 3 cut(s) 934, 1853, 2278
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.