MD09G1094400.v1.1

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0000564
Physical Location & Seq
Forward (+)
6861657 .. 6871001
9345 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1094400.v1.1.491

Sequence Viewer

Length: 2457 bp
ATGAATACCAAACCAAACTCAAAGTTCTCGTTATCCGTTATTTTATTATGTGCATTTCTCGAGTCCCATGTTTCCCTTGCAGCTGACACCATCAGCGAAAACCACTCTCTCTCGGGCGATCAAACCGTTGTCTCTGCGGGTGGGATGTTTGAGCTAGGTTTCTTCAAACCAGGTCAGCTTTCAAACTACTATATAGGCATATGGTACTCCAAGCAAGTAGTATCTGAGATGACTGTTGTTTGGGTAGCAAATAGGGAAACACCAGTTTCTGATATATTTTCTTCGGCATTGAGCATCTTAGATGGTAATTTAGTTCTCTTTGACGAGTCCAAAACCCCGGTTTGGTCAACAAATTTAAACTCCACCACCAATTCTGGTTCTGTACAAGCAGTTCTTTTGGACAGCGGAAACCTTGTCTTGATAGCCGGCTCTAGTAATAATACATCAGAGCCTTTATGGCAAAGCTTTGATCATCCAACCCATGCTTTGCTACCAGGAGGTAAACTTGGATTCAACAAAATTACAAATCATACCCAATTTCTCACTTCATGGAAGAATTTGGAGGATCCTGCACCAGGTCTTTACTCTCTTGAGCTAGCTCCGGATGAAAGCAATTCATATATCTTATTGTGGAATAGGTCTAGACAGTATTGGACCAGCGGACCATGGAATGAAAGTTTGCGTAATTTCAACATGGTTCCTGAATTGAGGCTATTTAATGACTTCAATTATAGTTTTGTTACGAATGAGAACGAAAGTTATCTCACATATTCTATTAACAATCCTAAAATTGTAACTCGAGGCTTGATGTCTGCCTCGGGACAGGTTCAGCAACTAACATGGTTGGAGGCTTCCAAGCAGTGGAATGTGTTTTGGTCTCAACCAAGACAGTGTGATGTTTATGCTTTTTGTGGGGCTTTCTCCAGTTGCAAACCCACATCCTCGAACTACTGCAAATGTTTGAAGGGCTTTGAGCCAAACCGACAGAGTGATTGGGACTTGCAGATTTATTCCGGTGGATGTTCAAGAAGAACCAGTGTTAAGTGTGGGAATGCTACGGGAGACGGGTTTCTAGAAAAAAATAGCAAGTCCTTCCTTGAAAATAGCCAATATGAACCAGCTCCTAGTTTCGAAAGTTGTAGATCAGCCTGCTTAAATGAATGCCAGTGCACTGCTTATGTTTATGACAGCAGCAATGGATGTTCAATATGGCATGGAGATGTCTTGAATCTGCAAGAATTCGAAGCAAATGATGGTGATGGAAGAATTTTATACATCAAAGCTGCAGCTTCCGACATTGAGAAAAAACGTGTAATTAAGTTGTCCCCTTTGATTGCAATAGTCTCAACAATCACAGGTTTGCTTGTTGTCATTTTGGGCTATTTCTTATGGAAGAAAACATTGGGAACAAAAAGTGAAGTTGCTGCTGGAGGTGAAATGAATGATGCAGAACTGCCGATCTTTGGTTTAAAGGCTATAATAGCTGCTACAAACAACTTCGCTGAAGCTAATAAACTCGGAGAGGGAGGATTTGGCCCTGTCTACAAGGGAATTTTGGCTGAAAATCAAGAAGTAGCCATAAAAAGGTTATCAAAAAAGTCAGGCCAAGGACACGAGGAGTTCATGAATGAGTTAAAACTTATTGCCAAGCTCCAGCATACCAATCTCGTTAGGCTCCTGGGTTGCTGTATTGAAGAGGAGGAAATGATATTGATCTACGAGTACATGCCCAATCGGAGCTTGGACAAACTTTTGTTTGATGCATCAGAAAAAAGAGAATTAGATTGGGGAAGACGTTTTCGAATTATAGAAGGCATTGCTCAAGGAGTACTTTATATCCACAAGTACTCCAGATTGAAAATCATCCACAGGGACCTAAAAGCAAGTAACATACTGTTGGATGGAGCCTTGAACCCCAAAATTTCAGACTTTGGAATGGCAAGGATTTTCGGGATAAATCAGACTGAAGCAAATACCAATAGGGTTGTTGGCACATATGGCTACATGTCACCCGAATATGCGAGATATGGTCATTTCTCTGAGAAATTAGATGTATTCAGCTTCGGAGTGTTGTTGTTGGAGATTGTGAGCGGAAAGAAGAATGCTGCTTTTTATCGCCTTGAACATTCACCAACTCTTGCTGGATGGGCATGGGAGTTGTGGAAAGACGGAAGAGGAATGGAGGTGATTGATGAATCAGTAAGGGAAACATACCGGCTCGATGAAGCTTTGAAGTGTATCCATGTAGGGTTTTTGTGTGTTCAAGAAGCTCCAACTGATCGGCCAACAATGTCTTCGGTAATTCGTATGCTGCAGAGCAATGAATCTACATCTCTTCCACCTTTCAAAGAACCTGCCTTTTCAACACACAAAAATTCCAATCCTGTTTGTTCTTCTCAAATGCCTACCATTTTTTCCTACAACACAGTCACCATGACTTTGCCAGAAGGTCGATAG

Protein Analysis

819

Amino Acids

91.37

Weight (kDa)

5.82

Isoelectric Point (pI)

41.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 78 - 185 1.9e-33 D-mannose binding lectin
S_locus_glycop PF00954 218 - 327 1.6e-28 S-locus glycoprotein domain
PAN_2 PF08276 352 - 410 2.2e-10 PAN-like domain
Pkinase PF00069 501 - 768 1.3e-47 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 504 - 771 3e-49 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000564)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g44064 FvH4_6g44064 FvH4_6g44100 FvH4_6g44101 FvH4_6g51830
malus_domestica MD09G1094400.v1.1 MD09G1094800.v1.1 MD09G1095200.v1.1 MD09G1095900.v1.1 MD09G1096000.v1.1 MD09G1096200.v1.1 MD17G1083900.v1.1 MD17G1088500.v1.1
prunus_persica Prupe.3G224900_v2.0.a1 Prupe.3G224900_v2.0.a1 Prupe.3G224900_v2.0.a1 Prupe.3G224900_v2.0.a1 Prupe.3G224900_v2.0.a1 Prupe.3G224900_v2.0.a1 Prupe.3G225100_v2.0.a1 Prupe.3G225100_v2.0.a1 Prupe.3G225100_v2.0.a1 Prupe.3G225200_v2.0.a1 Prupe.3G225200_v2.0.a1
pyrus_communis pycom09g01920 pycom09g01930 pycom09g01940 pycom09g01950 pycom09g02000 pycom09g02050 pycom09g02070 pycom17g08540
rosa_chinensis RchiOBHm_Chr1g0318601 RchiOBHm_Chr1g0318761 RchiOBHm_Chr1g0342541 RchiOBHm_Chr2g0160651 RchiOBHm_Chr2g0160831 RchiOBHm_Chr4g0405661 RchiOBHm_Chr4g0405691 RchiOBHm_Chr4g0405701 RchiOBHm_Chr7g0238591
rosa_laevigata RLG00000002292 RLG00000008826 RLG00000008828 RLG00000008829 RLG00000021243 RLG00000021245 RLG00000030522
rosa_multiflora Rmu_co8432573.1_g000001 Rmu_sc0001200.1_g000041 Rmu_sc0001200.1_g000043 Rmu_sc0002545.1_g000017 Rmu_sc0002773.1_g000017 Rmu_sc0012550.1_g000008
rosa_roxburghii Rroxscaffold_2G00089500 Rroxscaffold_5G00350130 Rroxscaffold_5G00350180
rosa_rugosa Rorug01G0022300 Rorug04G0061600
rosa_samantha Rh1AG035300 Rh1CG034300 Rh1DG036100 Rh1DG149100 Rh2AG550900 Rh2BG564200 Rh2BG565500 Rh2CG535300 Rh2DG574400 Rh4AG133100 Rh4AG133200 Rh4AG133300 Rh4BG128200 Rh4CG141000 Rh4DG127700 Rh4DG127900
rosa_wichuraiana Rw2G045540 Rw2G045650 Rw4G010790 Rw4G010800 Rw4G010810 Rw4G010830 Rw7G027620 Rw7G027630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 2362
AccB7I CCANNNNNTGG 1 cut(s) 861
AccBSI CCGCTC 1 cut(s) 2091
AccI GTMKAC 1 cut(s) 1542
AccIII TCCGGA 1 cut(s) 601
AciI CCGC 4 cut(s) 137, 405, 660, 2091
AclWI GGATC 2 cut(s) 560, 573
AcoI YGGCCR 1 cut(s) 2282
AcsI RAATTY 7 cut(s) 352, 556, 1238, 1266, 1551, 1920, 2374
AcuI CTGAAG 2 cut(s) 1524, 1986
AfaI GTAC 5 cut(s) 206, 384, 1724, 1830, 1847
AfiI CCNNNNNNNGG 5 cut(s) 342, 575, 861, 1463, 1584
AflIII ACRYGT 2 cut(s) 1309, 2004
AjnI CCWGG 4 cut(s) 169, 493, 574, 1677
AjuI GAANNNNNNNTTGG 4 cut(s) 1385, 1417, 2278, 2310
Alw21I GWGCWC 1 cut(s) 1172
Alw26I GTCTC 4 cut(s) 136, 882, 1056, 1348
Alw44I GTGCAC 1 cut(s) 1168
AlwI GGATC 2 cut(s) 560, 573
AlwNI CAGNNNCTG 1 cut(s) 269
Ama87I CYCGRG 4 cut(s) 59, 112, 798, 817
Aor13HI TCCGGA 1 cut(s) 601
AoxI GGCC 3 cut(s) 1534, 1603, 2282
ApaLI GTGCAC 1 cut(s) 1168
ApeKI GCWGC 8 cut(s) 80, 1191, 1283, 1286, 1424, 1484, 2105, 2311
ApoI RAATTY 7 cut(s) 352, 556, 1238, 1266, 1551, 1920, 2374
AspS9I GGNCC 4 cut(s) 654, 662, 1535, 1873
AsuC2I CCSGG 1 cut(s) 338
AsuHPI GGTGA 6 cut(s) 1268, 1445, 2001, 2121, 2197, 2422
AsuII TTCGAA 3 cut(s) 1131, 1242, 1801
AsuNHI GCTAGC 1 cut(s) 595
AvaI CYCGRG 4 cut(s) 59, 112, 798, 817
AvaII GGWCC 3 cut(s) 654, 662, 1873
BaeGI GKGCMC 1 cut(s) 1172
BamHI GGATCC 1 cut(s) 565
BauI CACGAG 1 cut(s) 1613
BbsI GAAGAC 2 cut(s) 1798, 2286
Bbv12I GWGCWC 1 cut(s) 1172
BbvI GCAGC 8 cut(s) 92, 1203, 1270, 1298, 1411, 1471, 2092, 2298
BccI CCATC 6 cut(s) 98, 296, 1247, 1253, 1895, 2139
BciT130I CCWGG 4 cut(s) 171, 495, 576, 1679
BciVI GTATCC 1 cut(s) 2249
BclI TGATCA 1 cut(s) 469
BcnI CCSGG 1 cut(s) 338
BcoDI GTCTC 4 cut(s) 136, 882, 1056, 1348
BfaI CTAG 6 cut(s) 155, 432, 596, 642, 1073, 1125
BfmI CTRYAG 2 cut(s) 1284, 2312
BfuAI ACCTGC 1 cut(s) 2362
BfuI GTATCC 1 cut(s) 2249
BglI GCCNNNNNGGC 1 cut(s) 457
BisI GCNGC 8 cut(s) 81, 1192, 1284, 1287, 1425, 1485, 2106, 2312
BlsI GCNGC 8 cut(s) 82, 1193, 1285, 1288, 1426, 1486, 2107, 2313
BmcAI AGTACT 2 cut(s) 1830, 1847
Bme1390I CCNGG 5 cut(s) 171, 338, 495, 576, 1679
Bme18I GGWCC 3 cut(s) 654, 662, 1873
BmeT110I CYCGRG 4 cut(s) 59, 112, 798, 817
BmgT120I GGNCC 4 cut(s) 654, 662, 1535, 1873
BmiI GGNNCC 5 cut(s) 567, 699, 1676, 1874, 1906
BmrFI CCNGG 5 cut(s) 171, 338, 495, 576, 1679
BmsI GCATC 4 cut(s) 303, 1435, 1750, 1772
BmtI GCTAGC 1 cut(s) 599
BpiI GAAGAC 2 cut(s) 1798, 2286
BpmI CTGGAG 4 cut(s) 907, 1449, 1637, 1834
Bpu14I TTCGAA 3 cut(s) 1131, 1242, 1801
BpuEI CTTGAG 2 cut(s) 611, 1806
BpuMI CCSGG 1 cut(s) 338
BsaBI GATNNNNATC 1 cut(s) 1712
BsaI GGTCTC 1 cut(s) 882
BsaJI CCNNGG 5 cut(s) 336, 665, 816, 1606, 1678
BsaWI WCCGGW 2 cut(s) 601, 1013
BsaXI ACNNNNNCTCC 2 cut(s) 1832, 1862
Bsc4I CCNNNNNNNGG 5 cut(s) 342, 575, 861, 1463, 1584
Bse118I RCCGGY 2 cut(s) 425, 2214
Bse1I ACTGG 4 cut(s) 263, 924, 1035, 1165
Bse3DI GCAATG 3 cut(s) 1201, 1815, 2326
Bse8I GATNNNNATC 1 cut(s) 1712
BseAI TCCGGA 1 cut(s) 601
BseBI CCWGG 4 cut(s) 171, 495, 576, 1679
BseDI CCNNGG 5 cut(s) 336, 665, 816, 1606, 1678
BseGI GGATG 9 cut(s) 150, 472, 610, 938, 1025, 1205, 1863, 1906, 2150
BseJI GATNNNNATC 1 cut(s) 1712
BseLI CCNNNNNNNGG 5 cut(s) 342, 575, 861, 1463, 1584
BseMI GCAATG 3 cut(s) 1201, 1815, 2326
BseMII CTCAG 2 cut(s) 216, 2031
BseNI ACTGG 4 cut(s) 263, 924, 1035, 1165
BseRI GAGGAG 2 cut(s) 1631, 1712
BseSI GKGCMC 1 cut(s) 1172
BseXI GCAGC 8 cut(s) 92, 1203, 1270, 1298, 1411, 1471, 2092, 2298
BsgI GTGCAG 1 cut(s) 555
BshFI GGCC 3 cut(s) 1536, 1605, 2284
BsiHKAI GWGCWC 1 cut(s) 1172
BsiHKCI CYCGRG 4 cut(s) 59, 112, 798, 817
BsiSI CCGG 5 cut(s) 338, 426, 602, 1014, 2215
BslFI GGGAC 5 cut(s) 49, 834, 1010, 1309, 1886
BslI CCNNNNNNNGG 5 cut(s) 342, 575, 861, 1463, 1584
BsmAI GTCTC 4 cut(s) 136, 882, 1056, 1348
BsmBI CGTCTC 1 cut(s) 1056
BsmFI GGGAC 5 cut(s) 49, 834, 1010, 1309, 1886
BsmI GAATGC 3 cut(s) 1057, 1166, 2107
BsnI GGCC 3 cut(s) 1536, 1605, 2284
Bso31I GGTCTC 1 cut(s) 882
BsoBI CYCGRG 4 cut(s) 59, 112, 798, 817
Bsp119I TTCGAA 3 cut(s) 1131, 1242, 1801
Bsp1286I GDGCHC 1 cut(s) 1172
Bsp13I TCCGGA 1 cut(s) 601
Bsp1407I TGTACA 1 cut(s) 382
Bsp143I GATC 7 cut(s) 118, 469, 565, 1142, 1458, 1713, 2278
Bsp19I CCATGG 1 cut(s) 665
BspACI CCGC 4 cut(s) 137, 405, 660, 2091
BspANI GGCC 3 cut(s) 1536, 1605, 2284
BspCNI CTCAG 2 cut(s) 217, 2032
BspEI TCCGGA 1 cut(s) 601
BspHI TCATGA 1 cut(s) 1623
BspLI GGNNCC 5 cut(s) 567, 699, 1676, 1874, 1906
BspMAI CTGCAG 2 cut(s) 1288, 2316
BspMI ACCTGC 1 cut(s) 2362
BspOI GCTAGC 1 cut(s) 599
BspPI GGATC 2 cut(s) 560, 573
BspT104I TTCGAA 3 cut(s) 1131, 1242, 1801
BspTNI GGTCTC 1 cut(s) 882
BsrBI CCGCTC 1 cut(s) 2091
BsrDI GCAATG 3 cut(s) 1201, 1815, 2326
BsrFI RCCGGY 2 cut(s) 425, 2214
BsrGI TGTACA 1 cut(s) 382
BsrI ACTGG 4 cut(s) 263, 924, 1035, 1165
BssAI RCCGGY 2 cut(s) 425, 2214
BssECI CCNNGG 5 cut(s) 336, 665, 816, 1606, 1678
BssMI GATC 7 cut(s) 118, 469, 565, 1142, 1458, 1713, 2278
BssSI CACGAG 1 cut(s) 1613
BssT1I CCWWGG 2 cut(s) 665, 1606
Bst2BI CACGAG 1 cut(s) 1613
Bst2UI CCWGG 4 cut(s) 171, 495, 576, 1679
Bst4CI ACNGT 6 cut(s) 127, 235, 648, 891, 1896, 2428
Bst6I CTCTTC 3 cut(s) 1689, 2167, 2340
BstAUI TGTACA 1 cut(s) 382
BstBI TTCGAA 3 cut(s) 1131, 1242, 1801
BstC8I GCNNGC 3 cut(s) 427, 597, 1150
BstDEI CTNAG 3 cut(s) 225, 298, 2040
BstDSI CCRYGG 1 cut(s) 665
BstENI CCTNNNNNAGG 1 cut(s) 573
BstF5I GGATG 9 cut(s) 150, 472, 610, 938, 1025, 1205, 1863, 1906, 2150
BstKTI GATC 7 cut(s) 121, 472, 568, 1145, 1461, 1716, 2281
BstMAI GTCTC 4 cut(s) 136, 882, 1056, 1348
BstMBI GATC 7 cut(s) 118, 469, 565, 1142, 1458, 1713, 2278
BstMWI GCNNNNNNNGC 4 cut(s) 457, 1481, 1998, 2147
BstNI CCWGG 4 cut(s) 171, 495, 576, 1679
BstNSI RCATGY 2 cut(s) 1729, 2008
BstSCI CCNGG 5 cut(s) 169, 336, 493, 574, 1677
BstSFI CTRYAG 2 cut(s) 1284, 2312
BstSLI GKGCMC 1 cut(s) 1172
BstV1I GCAGC 8 cut(s) 92, 1203, 1270, 1298, 1411, 1471, 2092, 2298
BstV2I GAAGAC 2 cut(s) 1798, 2286
BstX2I RGATCY 1 cut(s) 565
BstYI RGATCY 1 cut(s) 565
BsuI GTATCC 1 cut(s) 2249
BsuRI GGCC 3 cut(s) 1536, 1605, 2284
BtgI CCRYGG 1 cut(s) 665
BtsCI GGATG 9 cut(s) 150, 472, 610, 938, 1025, 1205, 1863, 1906, 2150
BtsI GCAGTG 2 cut(s) 866, 1170
BtsIMutI CAGTG 5 cut(s) 866, 896, 1042, 1170, 1172
BveI ACCTGC 1 cut(s) 2362
Cac8I GCNNGC 3 cut(s) 427, 597, 1150
CaiI CAGNNNCTG 1 cut(s) 269
CciI TCATGA 1 cut(s) 1623
Cfr10I RCCGGY 2 cut(s) 425, 2214
Cfr13I GGNCC 4 cut(s) 654, 662, 1535, 1873
CsiI ACCWGGT 2 cut(s) 169, 574
Csp6I GTAC 5 cut(s) 205, 383, 1723, 1829, 1846
CviQI GTAC 5 cut(s) 205, 383, 1723, 1829, 1846
DdeI CTNAG 3 cut(s) 225, 298, 2040
DpnI GATC 7 cut(s) 120, 471, 567, 1144, 1460, 1715, 2280
DpnII GATC 7 cut(s) 118, 469, 565, 1142, 1458, 1713, 2278
DraI TTTAAA 2 cut(s) 357, 1470
EaeI YGGCCR 1 cut(s) 2282
Eam1104I CTCTTC 3 cut(s) 1689, 2167, 2340
EarI CTCTTC 3 cut(s) 1689, 2167, 2340
Eco130I CCWWGG 2 cut(s) 665, 1606
Eco31I GGTCTC 1 cut(s) 882
Eco47I GGWCC 3 cut(s) 654, 662, 1873
Eco57I CTGAAG 2 cut(s) 1524, 1986
Eco88I CYCGRG 4 cut(s) 59, 112, 798, 817
EcoNI CCTNNNNNAGG 1 cut(s) 573
EcoO109I RGGNCCY 1 cut(s) 1873
EcoRI GAATTC 1 cut(s) 1238
EcoRII CCWGG 4 cut(s) 169, 493, 574, 1677
EcoT14I CCWWGG 2 cut(s) 665, 1606
EcoT22I ATGCAT 1 cut(s) 1765
ErhI CCWWGG 2 cut(s) 665, 1606
Esp3I CGTCTC 1 cut(s) 1056
FalI AAGNNNNNCTT 4 cut(s) 378, 410, 1815, 1847
FaqI GGGAC 5 cut(s) 49, 834, 1010, 1309, 1886
FauI CCCGC 1 cut(s) 130
FauNDI CATATG 2 cut(s) 200, 1996
FbaI TGATCA 1 cut(s) 469
FblI GTMKAC 1 cut(s) 1542
Fnu4HI GCNGC 8 cut(s) 81, 1192, 1284, 1287, 1425, 1485, 2106, 2312
FokI GGATG 9 cut(s) 157, 459, 617, 925, 1032, 1212, 1850, 1913, 2157
Fsp4HI GCNGC 8 cut(s) 81, 1192, 1284, 1287, 1425, 1485, 2106, 2312
FspBI CTAG 6 cut(s) 155, 432, 596, 642, 1073, 1125
GluI GCNGC 8 cut(s) 81, 1192, 1284, 1287, 1425, 1485, 2106, 2312
GsuI CTGGAG 4 cut(s) 907, 1449, 1637, 1834
HaeIII GGCC 3 cut(s) 1536, 1605, 2284
HapII CCGG 5 cut(s) 338, 426, 602, 1014, 2215
HincII GTYRAC 1 cut(s) 348
HindII GTYRAC 1 cut(s) 348
HindIII AAGCTT 2 cut(s) 463, 2226
HinfI GANTC 6 cut(s) 62, 326, 510, 1228, 2195, 2324
HpaII CCGG 5 cut(s) 338, 426, 602, 1014, 2215
HphI GGTGA 6 cut(s) 1268, 1445, 2001, 2121, 2197, 2422
Hpy166II GTNNAC 4 cut(s) 348, 503, 1170, 1543
Hpy8I GTNNAC 4 cut(s) 348, 503, 1170, 1543
HpyAV CCTTC 4 cut(s) 958, 1102, 1805, 2441
HpyCH4III ACNGT 6 cut(s) 127, 235, 648, 891, 1896, 2428
HpyCH4IV ACGT 2 cut(s) 1309, 1795
HpyF10VI GCNNNNNNNGC 4 cut(s) 457, 1481, 1998, 2147
HpyF3I CTNAG 3 cut(s) 225, 298, 2040
HpySE526I ACGT 2 cut(s) 1309, 1795
Kpn2I TCCGGA 1 cut(s) 601
KroI GCCGGC 1 cut(s) 425
KroNI GCCGGC 1 cut(s) 427
Ksp22I TGATCA 1 cut(s) 469
Kzo9I GATC 7 cut(s) 118, 469, 565, 1142, 1458, 1713, 2278
LmnI GCTCC 7 cut(s) 604, 1126, 1656, 1680, 1737, 1904, 2275
Lsp1109I GCAGC 8 cut(s) 92, 1203, 1270, 1298, 1411, 1471, 2092, 2298
LweI GCATC 4 cut(s) 303, 1435, 1750, 1772
MabI ACCWGGT 2 cut(s) 169, 574
MaeI CTAG 6 cut(s) 155, 432, 596, 642, 1073, 1125
MaeII ACGT 2 cut(s) 1309, 1795
MaeIII GTNAC 5 cut(s) 739, 793, 1886, 2007, 2428
MalI GATC 7 cut(s) 120, 471, 567, 1144, 1460, 1715, 2280
MbiI CCGCTC 1 cut(s) 2091
MboI GATC 7 cut(s) 118, 469, 565, 1142, 1458, 1713, 2278
MflI RGATCY 1 cut(s) 565
MhlI GDGCHC 1 cut(s) 1172
MlyI GAGTC 2 cut(s) 71, 335
MmeI TCCRAC 6 cut(s) 500, 825, 1317, 1878, 2058, 2297
Mph1103I ATGCAT 1 cut(s) 1765
MroI TCCGGA 1 cut(s) 601
MroNI GCCGGC 1 cut(s) 425
MseI TTAA 8 cut(s) 356, 717, 777, 1041, 1154, 1317, 1469, 1634
MslI CAYNNNNRTG 1 cut(s) 1218
MspA1I CMGCKG 3 cut(s) 83, 405, 660
MspI CCGG 5 cut(s) 338, 426, 602, 1014, 2215
MspR9I CCNGG 5 cut(s) 171, 338, 495, 576, 1679
Mva1269I GAATGC 3 cut(s) 1057, 1166, 2107
MvaI CCWGG 4 cut(s) 171, 495, 576, 1679
MwoI GCNNNNNNNGC 4 cut(s) 457, 1481, 1998, 2147
NaeI GCCGGC 1 cut(s) 427
NciI CCSGG 1 cut(s) 338
NcoI CCATGG 1 cut(s) 665
NdeI CATATG 2 cut(s) 200, 1996
NdeII GATC 7 cut(s) 118, 469, 565, 1142, 1458, 1713, 2278
NgoMIV GCCGGC 1 cut(s) 425
NheI GCTAGC 1 cut(s) 595
NlaIV GGNNCC 5 cut(s) 567, 699, 1676, 1874, 1906
NmuCI GTSAC 2 cut(s) 2007, 2428
NsiI ATGCAT 1 cut(s) 1765
NspI RCATGY 2 cut(s) 1729, 2008
NspV TTCGAA 3 cut(s) 1131, 1242, 1801
PaeR7I CTCGAG 2 cut(s) 59, 798
PagI TCATGA 1 cut(s) 1623
PciI ACATGT 1 cut(s) 2004
PctI GAATGC 3 cut(s) 1057, 1166, 2107
PdiI GCCGGC 1 cut(s) 427
PfeI GAWTC 4 cut(s) 510, 1228, 2195, 2324
PflMI CCANNNNNTGG 1 cut(s) 861
PkrI GCNGC 8 cut(s) 82, 1193, 1285, 1288, 1426, 1486, 2107, 2313
PleI GAGTC 2 cut(s) 70, 334
PpsI GAGTC 2 cut(s) 70, 334
PpuMI RGGWCCY 1 cut(s) 1873
PscI ACATGT 1 cut(s) 2004
Psp5II RGGWCCY 1 cut(s) 1873
Psp6I CCWGG 4 cut(s) 169, 493, 574, 1677
PspGI CCWGG 4 cut(s) 169, 493, 574, 1677
PspN4I GGNNCC 5 cut(s) 567, 699, 1676, 1874, 1906
PspPI GGNCC 4 cut(s) 654, 662, 1535, 1873
PspPPI RGGWCCY 1 cut(s) 1873
PspXI VCTCGAGB 1 cut(s) 798
PsrI GAACNNNNNNTAC 2 cut(s) 375, 407
PstI CTGCAG 2 cut(s) 1288, 2316
PstNI CAGNNNCTG 1 cut(s) 269
PsuI RGATCY 1 cut(s) 565
PvuII CAGCTG 1 cut(s) 83
RsaI GTAC 5 cut(s) 206, 384, 1724, 1830, 1847
RsaNI GTAC 5 cut(s) 205, 383, 1723, 1829, 1846
RseI CAYNNNNRTG 1 cut(s) 1218
SaqAI TTAA 8 cut(s) 356, 717, 777, 1041, 1154, 1317, 1469, 1634
SatI GCNGC 8 cut(s) 81, 1192, 1284, 1287, 1425, 1485, 2106, 2312
Sau3AI GATC 7 cut(s) 118, 469, 565, 1142, 1458, 1713, 2278
Sau96I GGNCC 4 cut(s) 654, 662, 1535, 1873
ScaI AGTACT 2 cut(s) 1830, 1847
SchI GAGTC 2 cut(s) 71, 335
ScrFI CCNGG 5 cut(s) 171, 338, 495, 576, 1679
SduI GDGCHC 1 cut(s) 1172
SexAI ACCWGGT 2 cut(s) 169, 574
SfaNI GCATC 4 cut(s) 303, 1435, 1750, 1772
SfcI CTRYAG 2 cut(s) 1284, 2312
Sfr274I CTCGAG 2 cut(s) 59, 798
SfuI TTCGAA 3 cut(s) 1131, 1242, 1801
SinI GGWCC 3 cut(s) 654, 662, 1873
SlaI CTCGAG 2 cut(s) 59, 798
SmiMI CAYNNNNRTG 1 cut(s) 1218
SmlI CTYRAG 4 cut(s) 59, 590, 798, 1821
SmoI CTYRAG 4 cut(s) 59, 590, 798, 1821
SsiI CCGC 4 cut(s) 137, 405, 660, 2091
SspMI CTAG 6 cut(s) 155, 432, 596, 642, 1073, 1125
StyD4I CCNGG 5 cut(s) 169, 336, 493, 574, 1677
StyI CCWWGG 2 cut(s) 665, 1606
TaaI ACNGT 6 cut(s) 127, 235, 648, 891, 1896, 2428
TaiI ACGT 2 cut(s) 1312, 1798
TaqI TCGA 8 cut(s) 60, 799, 944, 1131, 1242, 1801, 2220, 2452
TatI WGTACW 4 cut(s) 382, 1722, 1828, 1845
TfiI GAWTC 4 cut(s) 510, 1228, 2195, 2324
Tru1I TTAA 8 cut(s) 356, 717, 777, 1041, 1154, 1317, 1469, 1634
Tru9I TTAA 8 cut(s) 356, 717, 777, 1041, 1154, 1317, 1469, 1634
TscAI CASTG 5 cut(s) 866, 896, 1042, 1172, 1177
TseFI GTSAC 2 cut(s) 2007, 2428
TseI GCWGC 8 cut(s) 80, 1191, 1283, 1286, 1424, 1484, 2105, 2311
Tsp45I GTSAC 2 cut(s) 2007, 2428
TspGWI ACGGA 2 cut(s) 25, 2184
TspRI CASTG 5 cut(s) 866, 896, 1042, 1172, 1177
Van91I CCANNNNNTGG 1 cut(s) 861
VneI GTGCAC 1 cut(s) 1168
VpaK11BI GGWCC 3 cut(s) 654, 662, 1873
XagI CCTNNNNNAGG 1 cut(s) 573
XapI RAATTY 7 cut(s) 352, 556, 1238, 1266, 1551, 1920, 2374
XbaI TCTAGA 2 cut(s) 641, 1072
XceI RCATGY 2 cut(s) 1729, 2008
XcmI CCANNNNNNNNNTGG 1 cut(s) 1738
XhoI CTCGAG 2 cut(s) 59, 798
XmiI GTMKAC 1 cut(s) 1542
XspI CTAG 6 cut(s) 155, 432, 596, 642, 1073, 1125
ZrmI AGTACT 2 cut(s) 1830, 1847
Zsp2I ATGCAT 1 cut(s) 1765
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.