pycom09g02070

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0000564
Physical Location & Seq
Forward (+)
1513980 .. 1516497
2518 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g02070.1

Sequence Viewer

Length: 2025 bp
ATGAATACCAAACCAAACTCAAAGTTCGTGTTCTCCGTTATTTTATTATGTGCATTTCTCGAGTACCATGTTTACCTAGCAGCTGCCACCATCAGCGAAAACAAATCTCTCTCGGGCAATCAAACCATTTCAGGTGGGATTTTTGAGCTCGGTTTCTTCAAACCAGGTAACCTTTCAAACTTCTACATAGGCATATGGTACACCGACCGAGTAGTATCCGAGAGGACCATTGTTTGGGTGGCAAACAGGGAAAAACCAGTTTCCGATATATTTTCTTCGGTTCTAATGATTTCAGATGGTAATTTAGTCCTGCTTAACGAGTCCAAAGAGCTCATTTGGTCAACGAATTTAACCTCCTCCTCCTCCTCCGCCACTGCACTTCTTTTGGATACCGGAAACCTGGTCTTGAGAGCCGGGTCTAGTAATAATTTATCAGAGCTGTTATGGCAAAGCTTTGATCATCCAACGCATACTTGGCTGCCAGGAGGTAGAATTGGGTTTAACAATGTTACCAAACAAACCCAAATTCTTACTTCATGGAAGAGTTCGGAGGATCCTGCACCGGGTCTTTTCTCGCTCGAGTTAGACCCGAACGGAAGCAGTGCGTTTTTCATAATGTGGAATAGGTCTAGGCAGAATTGGACTAGTGGGTCATGGGACGCAAAATTAAGGGTTTTCCGCTTGGTTAGTACAGGGTTCAATACAAAAATATACAACCTCAGCTATGTTACGAACGCAACAGAAAGTTATTTCACCTATTCTATTTATGATCCTAGTAGAATATCTCGATTCGTGATGCTTACTTCGGGCCAGATTCAACAGCTGACATGGATGGAGAATTCCACGCAATGGAATTTGGTTTGGTCTCAACAGAGAAAGCAATGCGAAGTTTATGATTTGTGTGGGGCCTTCGGAAGTTGCAATGAGGTAAGCCCCCTCGCCTGTAATTGCTTGGAAGGTTTTGAGCCGAAGGTGAAGAGAGATTGGGATTTAAAGGATTACTCTGGTGGGTGTTCGAGAAAAACCGATCTGCATTGTGGAAATGCTACTAGTGCGAGCGGAAAGCCAGAGTGGTTTCTTCAACTTCGTACCATGTCATTGCCTAAAAATAATTACACTATGGAGGTTAGGAGTATTACCGAATGTGAAGTGTTCTGCCTTATAGGCTGTTCTTGCACTGCTTATGCTTATGACAGCAGCGGATGTTCGATTTGGAACGGAGAACTCTTGGATTTGCAACAACCAACATCAAGTGACGACCAGGGAAGAACTTTATACCTCAGACTTGCAGCTTCTGAGTTAGAGAGTTCTCAAAGTAACAAGGGATTGAATGTTGGTGTTGTGATTGCAATTGTCTCAATAATCACAGGTTTGCTGGTTGTCATTTTTGGCTATTACTTACTGAAGAAAACATTGGAAAAGAAAAGAGAGCATAGGAAAAGAAATGGTGAAAAGAACGATGCAGAACTGCCTATCTTTGGTTTAAGGGCTATAATAGCTGCTACAAACAATTTCATCGAAGCTAATAAACTCGGAGAGGGAGGATTTGGCCCTGTCTACAAGGGAATTTTGGGTGAAAATCAAGAAGTAGCCATAAAAAGGTTATCAAAAAAGTCAGGCCAAGGACAACAGGAGTTCATGAATGAGTTAAAACTTATTGCCAAGCTCCAACATACCAATCTCGTTAGGCTCTTGGGTTGCTGTCTTGAAGACGAGGAAATGATATTGATCTACGAGTACATGCCCAATCGGAGTTTGGACAAACTTTTGTTTGATGCATCTGAAAAAGGAGAATTAGATTGGGGAAGACGTTTTCAAATTATAGAAGGCATTGCTCAAGGAGTACTTTATATCCACAAGTATTCGAGATTGAAAATCATCCACAGGGACCTAAAAGCAAGTAACATACTATTGGATGGAGCCTTGAACCCCAAAATTTCAGACTTTGGAATGGCAAGGATTTTCGGGATGAATCAAACTGAAGCAAATACAAATAGGGTTGTTGGCACATACGGTTACATGTGA

Protein Analysis

675

Amino Acids

75.64

Weight (kDa)

7.45

Isoelectric Point (pI)

38.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 76 - 181 8.4e-34 D-mannose binding lectin
S_locus_glycop PF00954 214 - 324 4.1e-27 S-locus glycoprotein domain
PAN_2 PF08276 357 - 412 1.3e-13 PAN-like domain
Pkinase PF00069 507 - 674 2.4e-36 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 509 - 659 7.2e-35 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000564)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g44064 FvH4_6g44064 FvH4_6g44100 FvH4_6g44101 FvH4_6g51830
malus_domestica MD09G1094400.v1.1 MD09G1094800.v1.1 MD09G1095200.v1.1 MD09G1095900.v1.1 MD09G1096000.v1.1 MD09G1096200.v1.1 MD17G1083900.v1.1 MD17G1088500.v1.1
prunus_persica Prupe.3G224900_v2.0.a1 Prupe.3G224900_v2.0.a1 Prupe.3G224900_v2.0.a1 Prupe.3G224900_v2.0.a1 Prupe.3G224900_v2.0.a1 Prupe.3G224900_v2.0.a1 Prupe.3G225100_v2.0.a1 Prupe.3G225100_v2.0.a1 Prupe.3G225100_v2.0.a1 Prupe.3G225200_v2.0.a1 Prupe.3G225200_v2.0.a1
pyrus_communis pycom09g01920 pycom09g01930 pycom09g01940 pycom09g01950 pycom09g02000 pycom09g02050 pycom09g02070 pycom17g08540
rosa_chinensis RchiOBHm_Chr1g0318601 RchiOBHm_Chr1g0318761 RchiOBHm_Chr1g0342541 RchiOBHm_Chr2g0160651 RchiOBHm_Chr2g0160831 RchiOBHm_Chr4g0405661 RchiOBHm_Chr4g0405691 RchiOBHm_Chr4g0405701 RchiOBHm_Chr7g0238591
rosa_laevigata RLG00000002292 RLG00000008826 RLG00000008828 RLG00000008829 RLG00000021243 RLG00000021245 RLG00000030522
rosa_multiflora Rmu_co8432573.1_g000001 Rmu_sc0001200.1_g000041 Rmu_sc0001200.1_g000043 Rmu_sc0002545.1_g000017 Rmu_sc0002773.1_g000017 Rmu_sc0012550.1_g000008
rosa_roxburghii Rroxscaffold_2G00089500 Rroxscaffold_5G00350130 Rroxscaffold_5G00350180
rosa_rugosa Rorug01G0022300 Rorug04G0061600
rosa_samantha Rh1AG035300 Rh1CG034300 Rh1DG036100 Rh1DG149100 Rh2AG550900 Rh2BG564200 Rh2BG565500 Rh2CG535300 Rh2DG574400 Rh4AG133100 Rh4AG133200 Rh4AG133300 Rh4BG128200 Rh4CG141000 Rh4DG127700 Rh4DG127900
rosa_wichuraiana Rw2G045540 Rw2G045650 Rw4G010790 Rw4G010800 Rw4G010810 Rw4G010830 Rw7G027620 Rw7G027630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 649
AccB7I CCANNNNNTGG 2 cut(s) 234, 849
AccBSI CCGCTC 1 cut(s) 1059
AccI GTMKAC 1 cut(s) 1557
AciI CCGC 4 cut(s) 369, 679, 1059, 1200
AclWI GGATC 3 cut(s) 548, 561, 764
AcsI RAATTY 6 cut(s) 346, 525, 838, 853, 1566, 1935
AcuI CTGAAG 2 cut(s) 1424, 2001
AfaI GTAC 6 cut(s) 65, 200, 691, 1090, 1739, 1845
AfiI CCNNNNNNNGG 5 cut(s) 234, 563, 849, 1478, 1599
AflIII ACRYGT 1 cut(s) 2019
AhlI ACTAGT 2 cut(s) 644, 1049
AjnI CCWGG 4 cut(s) 163, 399, 481, 1260
AjuI GAANNNNNNNTTGG 2 cut(s) 1397, 1429
Alw21I GWGCWC 2 cut(s) 150, 333
Alw26I GTCTC 2 cut(s) 870, 1360
AlwI GGATC 3 cut(s) 548, 561, 764
AlwNI CAGNNNCTG 1 cut(s) 1295
Ama87I CYCGRG 3 cut(s) 59, 112, 578
AoxI GGCC 4 cut(s) 808, 906, 1549, 1618
ApeKI GCWGC 6 cut(s) 80, 83, 478, 1197, 1289, 1499
ApoI RAATTY 6 cut(s) 346, 525, 838, 853, 1566, 1935
AspS9I GGNCC 5 cut(s) 225, 808, 906, 1550, 1888
AsuC2I CCSGG 2 cut(s) 415, 564
AsuHPI GGTGA 4 cut(s) 745, 985, 1460, 1586
AvaI CYCGRG 3 cut(s) 59, 112, 578
AvaII GGWCC 2 cut(s) 225, 1888
BamHI GGATCC 1 cut(s) 553
BanII GRGCYC 2 cut(s) 150, 333
BbsI GAAGAC 2 cut(s) 1716, 1813
Bbv12I GWGCWC 2 cut(s) 150, 333
BbvCI CCTCAGC 1 cut(s) 719
BbvI GCAGC 6 cut(s) 70, 92, 465, 1209, 1301, 1486
BccI CCATC 4 cut(s) 98, 290, 826, 1910
BciT130I CCWGG 4 cut(s) 165, 401, 483, 1262
BciVI GTATCC 2 cut(s) 226, 382
BclI TGATCA 1 cut(s) 457
BcnI CCSGG 2 cut(s) 415, 564
BcoDI GTCTC 2 cut(s) 870, 1360
BcuI ACTAGT 2 cut(s) 644, 1049
BfaI CTAG 6 cut(s) 77, 420, 630, 645, 774, 1050
BfuI GTATCC 2 cut(s) 226, 382
BglI GCCNNNNNGGC 1 cut(s) 1164
BisI GCNGC 6 cut(s) 81, 84, 479, 1198, 1290, 1500
BlsI GCNGC 6 cut(s) 82, 85, 480, 1199, 1291, 1501
BmcAI AGTACT 1 cut(s) 1845
Bme1390I CCNGG 6 cut(s) 165, 401, 415, 483, 564, 1262
Bme18I GGWCC 2 cut(s) 225, 1888
BmeT110I CYCGRG 3 cut(s) 59, 112, 578
BmgT120I GGNCC 5 cut(s) 225, 808, 906, 1550, 1888
BmiI GGNNCC 4 cut(s) 555, 907, 1889, 1921
BmrFI CCNGG 6 cut(s) 165, 401, 415, 483, 564, 1262
BmsI GCATC 4 cut(s) 786, 1450, 1765, 1787
BpiI GAAGAC 2 cut(s) 1716, 1813
Bpu10I CCTNAGC 1 cut(s) 719
BpuEI CTTGAG 2 cut(s) 427, 1821
BpuMI CCSGG 2 cut(s) 415, 564
BsaBI GATNNNNATC 1 cut(s) 1727
BsaI GGTCTC 1 cut(s) 870
BsaJI CCNNGG 2 cut(s) 1261, 1621
BsaWI WCCGGW 1 cut(s) 392
Bsc4I CCNNNNNNNGG 5 cut(s) 234, 563, 849, 1478, 1599
Bse1I ACTGG 1 cut(s) 257
Bse3DI GCAATG 5 cut(s) 854, 887, 928, 1097, 1830
Bse8I GATNNNNATC 1 cut(s) 1727
BseBI CCWGG 4 cut(s) 165, 401, 483, 1262
BseDI CCNNGG 2 cut(s) 1261, 1621
BseGI GGATG 6 cut(s) 460, 837, 1208, 1878, 1921, 1974
BseJI GATNNNNATC 1 cut(s) 1727
BseLI CCNNNNNNNGG 5 cut(s) 234, 563, 849, 1478, 1599
BseMI GCAATG 5 cut(s) 854, 887, 928, 1097, 1830
BseMII CTCAG 3 cut(s) 733, 1287, 1294
BseNI ACTGG 1 cut(s) 257
BseRI GAGGAG 4 cut(s) 346, 349, 352, 355
BseXI GCAGC 6 cut(s) 70, 92, 465, 1209, 1301, 1486
BsgI GTGCAG 2 cut(s) 360, 543
Bsh1285I CGRYCG 1 cut(s) 208
BshFI GGCC 4 cut(s) 810, 908, 1551, 1620
BsiEI CGRYCG 1 cut(s) 208
BsiHKAI GWGCWC 2 cut(s) 150, 333
BsiHKCI CYCGRG 3 cut(s) 59, 112, 578
BsiSI CCGG 3 cut(s) 393, 414, 563
BslFI GGGAC 2 cut(s) 671, 1901
BslI CCNNNNNNNGG 5 cut(s) 234, 563, 849, 1478, 1599
BsmAI GTCTC 2 cut(s) 870, 1360
BsmFI GGGAC 2 cut(s) 671, 1901
BsnI GGCC 4 cut(s) 810, 908, 1551, 1620
Bso31I GGTCTC 1 cut(s) 870
BsoBI CYCGRG 3 cut(s) 59, 112, 578
Bsp1286I GDGCHC 2 cut(s) 150, 333
Bsp143I GATC 5 cut(s) 457, 553, 769, 1027, 1728
BspACI CCGC 4 cut(s) 369, 679, 1059, 1200
BspANI GGCC 4 cut(s) 810, 908, 1551, 1620
BspCNI CTCAG 3 cut(s) 732, 1288, 1293
BspHI TCATGA 1 cut(s) 1638
BspLI GGNNCC 4 cut(s) 555, 907, 1889, 1921
BspPI GGATC 3 cut(s) 548, 561, 764
BspTNI GGTCTC 1 cut(s) 870
BsrBI CCGCTC 1 cut(s) 1059
BsrDI GCAATG 5 cut(s) 854, 887, 928, 1097, 1830
BsrI ACTGG 1 cut(s) 257
BssECI CCNNGG 2 cut(s) 1261, 1621
BssMI GATC 5 cut(s) 457, 553, 769, 1027, 1728
BssT1I CCWWGG 1 cut(s) 1621
Bst2UI CCWGG 4 cut(s) 165, 401, 483, 1262
Bst4CI ACNGT 1 cut(s) 2015
Bst6I CTCTTC 2 cut(s) 536, 971
BstC8I GCNNGC 1 cut(s) 1057
BstDEI CTNAG 3 cut(s) 719, 1280, 1296
BstEII GGTNACC 1 cut(s) 167
BstF5I GGATG 6 cut(s) 460, 837, 1208, 1878, 1921, 1974
BstKTI GATC 5 cut(s) 460, 556, 772, 1030, 1731
BstMAI GTCTC 2 cut(s) 870, 1360
BstMBI GATC 5 cut(s) 457, 553, 769, 1027, 1728
BstMCI CGRYCG 1 cut(s) 208
BstMWI GCNNNNNNNGC 6 cut(s) 445, 475, 1052, 1164, 1173, 1496
BstNI CCWGG 4 cut(s) 165, 401, 483, 1262
BstNSI RCATGY 2 cut(s) 1744, 2023
BstPI GGTNACC 1 cut(s) 167
BstSCI CCNGG 6 cut(s) 163, 399, 413, 481, 562, 1260
BstV1I GCAGC 6 cut(s) 70, 92, 465, 1209, 1301, 1486
BstV2I GAAGAC 2 cut(s) 1716, 1813
BstX2I RGATCY 1 cut(s) 553
BstYI RGATCY 1 cut(s) 553
BsuI GTATCC 2 cut(s) 226, 382
BsuRI GGCC 4 cut(s) 810, 908, 1551, 1620
BtsCI GGATG 6 cut(s) 460, 837, 1208, 1878, 1921, 1974
BtsI GCAGTG 3 cut(s) 372, 607, 1176
BtsIMutI CAGTG 3 cut(s) 372, 607, 1176
Cac8I GCNNGC 1 cut(s) 1057
CaiI CAGNNNCTG 1 cut(s) 1295
CciI TCATGA 1 cut(s) 1638
Cfr13I GGNCC 5 cut(s) 225, 808, 906, 1550, 1888
CseI GACGC 1 cut(s) 668
CsiI ACCWGGT 2 cut(s) 163, 399
Csp6I GTAC 6 cut(s) 64, 199, 690, 1089, 1738, 1844
CviAII CATG 8 cut(s) 68, 537, 654, 828, 1093, 1639, 1741, 2020
CviQI GTAC 6 cut(s) 64, 199, 690, 1089, 1738, 1844
DdeI CTNAG 3 cut(s) 719, 1280, 1296
DpnI GATC 5 cut(s) 459, 555, 771, 1029, 1730
DpnII GATC 5 cut(s) 457, 553, 769, 1027, 1728
DraI TTTAAA 1 cut(s) 993
DrdI GACNNNNNNGTC 1 cut(s) 649
DseDI GACNNNNNNGTC 1 cut(s) 649
Eam1104I CTCTTC 2 cut(s) 536, 971
EarI CTCTTC 2 cut(s) 536, 971
EciI GGCGGA 1 cut(s) 358
Ecl136II GAGCTC 2 cut(s) 148, 331
Eco130I CCWWGG 1 cut(s) 1621
Eco24I GRGCYC 2 cut(s) 150, 333
Eco31I GGTCTC 1 cut(s) 870
Eco47I GGWCC 2 cut(s) 225, 1888
Eco53kI GAGCTC 2 cut(s) 148, 331
Eco57I CTGAAG 2 cut(s) 1424, 2001
Eco88I CYCGRG 3 cut(s) 59, 112, 578
Eco91I GGTNACC 1 cut(s) 167
EcoICRI GAGCTC 2 cut(s) 148, 331
EcoO109I RGGNCCY 2 cut(s) 906, 1888
EcoO65I GGTNACC 1 cut(s) 167
EcoRI GAATTC 1 cut(s) 838
EcoRII CCWGG 4 cut(s) 163, 399, 481, 1260
EcoT14I CCWWGG 1 cut(s) 1621
EcoT22I ATGCAT 1 cut(s) 1780
EcoT38I GRGCYC 2 cut(s) 150, 333
ErhI CCWWGG 1 cut(s) 1621
FaeI CATG 8 cut(s) 71, 540, 657, 831, 1096, 1642, 1744, 2023
FalI AAGNNNNNCTT 2 cut(s) 1830, 1862
FaqI GGGAC 2 cut(s) 671, 1901
FatI CATG 8 cut(s) 67, 536, 653, 827, 1092, 1638, 1740, 2019
FauNDI CATATG 1 cut(s) 194
FbaI TGATCA 1 cut(s) 457
FblI GTMKAC 1 cut(s) 1557
Fnu4HI GCNGC 6 cut(s) 81, 84, 479, 1198, 1290, 1500
FokI GGATG 6 cut(s) 447, 844, 1215, 1865, 1928, 1981
FriOI GRGCYC 2 cut(s) 150, 333
Fsp4HI GCNGC 6 cut(s) 81, 84, 479, 1198, 1290, 1500
FspBI CTAG 6 cut(s) 77, 420, 630, 645, 774, 1050
GluI GCNGC 6 cut(s) 81, 84, 479, 1198, 1290, 1500
HaeIII GGCC 4 cut(s) 810, 908, 1551, 1620
HapII CCGG 3 cut(s) 393, 414, 563
HgaI GACGC 1 cut(s) 668
Hin1II CATG 8 cut(s) 71, 540, 657, 831, 1096, 1642, 1744, 2023
HincII GTYRAC 1 cut(s) 342
HindII GTYRAC 1 cut(s) 342
HindIII AAGCTT 1 cut(s) 451
HinfI GANTC 4 cut(s) 320, 789, 814, 1972
HpaII CCGG 3 cut(s) 393, 414, 563
HphI GGTGA 4 cut(s) 745, 985, 1460, 1586
Hpy166II GTNNAC 4 cut(s) 73, 201, 342, 1558
Hpy8I GTNNAC 4 cut(s) 73, 201, 342, 1558
HpyAV CCTTC 4 cut(s) 919, 950, 964, 1820
HpyCH4III ACNGT 1 cut(s) 2015
HpyCH4IV ACGT 1 cut(s) 1810
HpyF10VI GCNNNNNNNGC 6 cut(s) 445, 475, 1052, 1164, 1173, 1496
HpyF3I CTNAG 3 cut(s) 719, 1280, 1296
HpySE526I ACGT 1 cut(s) 1810
Hsp92II CATG 8 cut(s) 71, 540, 657, 831, 1096, 1642, 1744, 2023
Ksp22I TGATCA 1 cut(s) 457
Kzo9I GATC 5 cut(s) 457, 553, 769, 1027, 1728
LmnI GCTCC 2 cut(s) 1671, 1919
Lsp1109I GCAGC 6 cut(s) 70, 92, 465, 1209, 1301, 1486
LweI GCATC 4 cut(s) 786, 1450, 1765, 1787
MabI ACCWGGT 2 cut(s) 163, 399
MaeI CTAG 6 cut(s) 77, 420, 630, 645, 774, 1050
MaeII ACGT 1 cut(s) 1810
MaeIII GTNAC 7 cut(s) 167, 508, 727, 1253, 1316, 1901, 2015
MalI GATC 5 cut(s) 459, 555, 771, 1029, 1730
MbiI CCGCTC 1 cut(s) 1059
MboI GATC 5 cut(s) 457, 553, 769, 1027, 1728
MboII GAAGA 9 cut(s) 148, 267, 553, 988, 1070, 1278, 1417, 1721, 1818
MfeI CAATTG 1 cut(s) 1350
MflI RGATCY 1 cut(s) 553
MhlI GDGCHC 2 cut(s) 150, 333
MlyI GAGTC 1 cut(s) 329
MmeI TCCRAC 2 cut(s) 488, 1693
Mph1103I ATGCAT 1 cut(s) 1780
MseI TTAA 7 cut(s) 315, 350, 501, 668, 992, 1484, 1649
MspA1I CMGCKG 3 cut(s) 83, 823, 1200
MspI CCGG 3 cut(s) 393, 414, 563
MspR9I CCNGG 6 cut(s) 165, 401, 415, 483, 564, 1262
MunI CAATTG 1 cut(s) 1350
MvaI CCWGG 4 cut(s) 165, 401, 483, 1262
MwoI GCNNNNNNNGC 6 cut(s) 445, 475, 1052, 1164, 1173, 1496
NciI CCSGG 2 cut(s) 415, 564
NdeI CATATG 1 cut(s) 194
NdeII GATC 5 cut(s) 457, 553, 769, 1027, 1728
NlaIII CATG 8 cut(s) 71, 540, 657, 831, 1096, 1642, 1744, 2023
NlaIV GGNNCC 4 cut(s) 555, 907, 1889, 1921
NmuCI GTSAC 1 cut(s) 1253
NsiI ATGCAT 1 cut(s) 1780
NspI RCATGY 2 cut(s) 1744, 2023
PaeR7I CTCGAG 2 cut(s) 59, 578
PagI TCATGA 1 cut(s) 1638
PciI ACATGT 1 cut(s) 2019
PcsI WCGNNNNNNNCGW 1 cut(s) 33
PfeI GAWTC 3 cut(s) 789, 814, 1972
PflMI CCANNNNNTGG 2 cut(s) 234, 849
PkrI GCNGC 6 cut(s) 82, 85, 480, 1199, 1291, 1501
PleI GAGTC 1 cut(s) 328
PpsI GAGTC 1 cut(s) 328
PpuMI RGGWCCY 1 cut(s) 1888
PscI ACATGT 1 cut(s) 2019
Psp124BI GAGCTC 2 cut(s) 150, 333
Psp5II RGGWCCY 1 cut(s) 1888
Psp6I CCWGG 4 cut(s) 163, 399, 481, 1260
PspEI GGTNACC 1 cut(s) 167
PspGI CCWGG 4 cut(s) 163, 399, 481, 1260
PspN4I GGNNCC 4 cut(s) 555, 907, 1889, 1921
PspPI GGNCC 5 cut(s) 225, 808, 906, 1550, 1888
PspPPI RGGWCCY 1 cut(s) 1888
PspXI VCTCGAGB 1 cut(s) 578
PstNI CAGNNNCTG 1 cut(s) 1295
PsuI RGATCY 1 cut(s) 553
PvuII CAGCTG 2 cut(s) 83, 823
RsaI GTAC 6 cut(s) 65, 200, 691, 1090, 1739, 1845
RsaNI GTAC 6 cut(s) 64, 199, 690, 1089, 1738, 1844
SacI GAGCTC 2 cut(s) 150, 333
SaqAI TTAA 7 cut(s) 315, 350, 501, 668, 992, 1484, 1649
SatI GCNGC 6 cut(s) 81, 84, 479, 1198, 1290, 1500
Sau3AI GATC 5 cut(s) 457, 553, 769, 1027, 1728
Sau96I GGNCC 5 cut(s) 225, 808, 906, 1550, 1888
ScaI AGTACT 1 cut(s) 1845
SchI GAGTC 1 cut(s) 329
ScrFI CCNGG 6 cut(s) 165, 401, 415, 483, 564, 1262
SduI GDGCHC 2 cut(s) 150, 333
SexAI ACCWGGT 2 cut(s) 163, 399
SfaNI GCATC 4 cut(s) 786, 1450, 1765, 1787
Sfr274I CTCGAG 2 cut(s) 59, 578
SinI GGWCC 2 cut(s) 225, 1888
SlaI CTCGAG 2 cut(s) 59, 578
SmlI CTYRAG 4 cut(s) 59, 406, 578, 1836
SmoI CTYRAG 4 cut(s) 59, 406, 578, 1836
SpeI ACTAGT 2 cut(s) 644, 1049
SsiI CCGC 4 cut(s) 369, 679, 1059, 1200
SspMI CTAG 6 cut(s) 77, 420, 630, 645, 774, 1050
SstI GAGCTC 2 cut(s) 150, 333
StyD4I CCNGG 6 cut(s) 163, 399, 413, 481, 562, 1260
StyI CCWWGG 1 cut(s) 1621
TaaI ACNGT 1 cut(s) 2015
TaiI ACGT 1 cut(s) 1813
TaqI TCGA 7 cut(s) 60, 579, 787, 1016, 1208, 1518, 1865
TaqII GACCGA 1 cut(s) 222
TatI WGTACW 3 cut(s) 689, 1737, 1843
TfiI GAWTC 3 cut(s) 789, 814, 1972
Tru1I TTAA 7 cut(s) 315, 350, 501, 668, 992, 1484, 1649
Tru9I TTAA 7 cut(s) 315, 350, 501, 668, 992, 1484, 1649
TscAI CASTG 3 cut(s) 379, 607, 1183
TseFI GTSAC 1 cut(s) 1253
TseI GCWGC 6 cut(s) 80, 83, 478, 1197, 1289, 1499
Tsp45I GTSAC 1 cut(s) 1253
TspDTI ATGAA 7 cut(s) 17, 525, 601, 1504, 1627, 1655, 1985
TspGWI ACGGA 3 cut(s) 25, 609, 1233
TspRI CASTG 3 cut(s) 379, 607, 1183
Van91I CCANNNNNTGG 2 cut(s) 234, 849
VpaK11BI GGWCC 2 cut(s) 225, 1888
XapI RAATTY 6 cut(s) 346, 525, 838, 853, 1566, 1935
XceI RCATGY 2 cut(s) 1744, 2023
XcmI CCANNNNNNNNNTGG 3 cut(s) 235, 471, 1753
XhoI CTCGAG 2 cut(s) 59, 578
XmiI GTMKAC 1 cut(s) 1557
XspI CTAG 6 cut(s) 77, 420, 630, 645, 774, 1050
ZrmI AGTACT 1 cut(s) 1845
Zsp2I ATGCAT 1 cut(s) 1780
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.