MD09G1094800.v1.1

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0000564
Physical Location & Seq
Forward (+)
6881647 .. 6883868
2222 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1094800.v1.1.491

Sequence Viewer

Length: 1719 bp
ATGAATACCAAACCAAACTCAAAGTTCGTGTTCTCCATTATTTTATTATGTGCATTTCTCGAGTCCCATGTTTCCCTTGCAGCTGACACCATCAGTGAAAACCAATCTCTCTCGGGCGATCAAACCATTGTCTCTGCAGGTGGGATGTTTGAGCTAGGTTTCTTCAAACCAGGTCAGCTTTCAAACTACTATATAGGCATGTGGTACTCCAAGCAAGTGGCATCTGAGATGACCGTTGTTTGGGTAGCAAATAGGGAAATACCGGTATCTGATAGATTTTCTTCCGTGTTAACCATCTTAGATGGTAATTTAGTTCTCTTGAACATGTCCAAAATCCCGGTTTGGTCAACAGATTTAAACTCCACTACCAGTTCTGGTTCTGTACTAGCAGTTCTTTTGGATAGCGGAAACCTTGTCTTGATAGCCGGCTCTAGTAATAATACATCAGAGCCTTTATGGCAAAGCCTTGATCATCCAACCCATACTTTGCTACCAGGAGGTAAACTTGGATTCAACAAAATTACGAATCATACCCAGATTCTCACTTCATGGAAGAGTCTGGAGGATCCTGCACCAGGTCTTTACTCTCTTGGGCTAGCTCCGGATGAAAGCAATTCATATATCTTATTGTGGAATAGGTCTAGACAGTATTGGACCAGCGGACCATGGAATGAAAGTCTGCATACTTTCAACGTGCTTCCTGAAATGAGGATAGATCATAACTTCATTTATAATTATGTTACAAATGAGAACGAAAGTTATTTCACATATTCTTCTAACAATCCTAAAATTGTATCTCAATACTCGATGTCTGCCTCAGGACAGATTCAGCTACTATGGATGGAGAATTCCAAGCAGTGGGAATTGTTTTGGTCTCAACCAAGACAGTGTGATGTTTATGCTTCTTGTGGGGCTTTCTCCAGTTGCAAACCCACATCCTTGAACTACTGCAAATGTTTGAAGGGCTTTGTGCCAAACCGGCAGAGTGATTGGGACTTGCAGATTTATTCTGGTGGATGTTCAAGAAGAACCAGTGTGACGTCCTGCTTAAATGAATGCCAGTGCACTGCTTATACTTATGGCAGCAGCAATGCATGTTCAATCTGGCATGGAGATGTCTTGAATCTGCATGAAGTTGAAGCAAATGAAGGTGATGGAAGTACTTTATACGTCAGAGCGGCAGCTTCTGACATCAAGGAAAAACGTGTAATTAAGCCGTCCCTTTGGATTGCAATAGTCTCAACAATCACTGGTTTAATTGTTGTCGTTTTTGGCTGTTTCTTATGGAAGAAAACTACGGGAAAGAAAAGAAAGCATAAGAAGAACCATACTGAGAGTAAAAGTAAATTTGATGTTGGAGGTGGAGGTGGAAAGGATGATGCAGAACTACCGATCTTCGGTTTAAGGGCTGTAGTAGCTGCTACAAACAACTTTGCTGAAGCTGATAAACTCGGCGAGGGAGGATTTGGCCCTGTTTACAAGGGAATTCTGGCTGAAAGTCAAGAAGTAGCCATTAAAAGGTTATCAAAAAGGTCAGGCCAAGGACACGAGGAGTTCATGAATGAATTAAAACTTATTGCCAAGCTCCAACATACCAGTCTCGTTAGGCTCTTGGGTTGCTGTCTTGAAGATGAGGAAATGATATTGATCTACGAGTACATGCCCAATCGGAGCTTAGACAAACTTTTATTTGGTAAATACAAGCTTATGACTTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

573

Amino Acids

63.67

Weight (kDa)

6.65

Isoelectric Point (pI)

41.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 78 - 185 1.9e-32 D-mannose binding lectin
S_locus_glycop PF00954 218 - 326 1.8e-26 S-locus glycoprotein domain
PAN_2 PF08276 347 - 375 8.4e-06 PAN-like domain
Pkinase PF00069 480 - 563 2.8e-15 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 482 - 563 9.7e-18 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000564)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g44064 FvH4_6g44064 FvH4_6g44100 FvH4_6g44101 FvH4_6g51830
malus_domestica MD09G1094400.v1.1 MD09G1094800.v1.1 MD09G1095200.v1.1 MD09G1095900.v1.1 MD09G1096000.v1.1 MD09G1096200.v1.1 MD17G1083900.v1.1 MD17G1088500.v1.1
prunus_persica Prupe.3G224900_v2.0.a1 Prupe.3G224900_v2.0.a1 Prupe.3G224900_v2.0.a1 Prupe.3G224900_v2.0.a1 Prupe.3G224900_v2.0.a1 Prupe.3G224900_v2.0.a1 Prupe.3G225100_v2.0.a1 Prupe.3G225100_v2.0.a1 Prupe.3G225100_v2.0.a1 Prupe.3G225200_v2.0.a1 Prupe.3G225200_v2.0.a1
pyrus_communis pycom09g01920 pycom09g01930 pycom09g01940 pycom09g01950 pycom09g02000 pycom09g02050 pycom09g02070 pycom17g08540
rosa_chinensis RchiOBHm_Chr1g0318601 RchiOBHm_Chr1g0318761 RchiOBHm_Chr1g0342541 RchiOBHm_Chr2g0160651 RchiOBHm_Chr2g0160831 RchiOBHm_Chr4g0405661 RchiOBHm_Chr4g0405691 RchiOBHm_Chr4g0405701 RchiOBHm_Chr7g0238591
rosa_laevigata RLG00000002292 RLG00000008826 RLG00000008828 RLG00000008829 RLG00000021243 RLG00000021245 RLG00000030522
rosa_multiflora Rmu_co8432573.1_g000001 Rmu_sc0001200.1_g000041 Rmu_sc0001200.1_g000043 Rmu_sc0002545.1_g000017 Rmu_sc0002773.1_g000017 Rmu_sc0012550.1_g000008
rosa_roxburghii Rroxscaffold_2G00089500 Rroxscaffold_5G00350130 Rroxscaffold_5G00350180
rosa_rugosa Rorug01G0022300 Rorug04G0061600
rosa_samantha Rh1AG035300 Rh1CG034300 Rh1DG036100 Rh1DG149100 Rh2AG550900 Rh2BG564200 Rh2BG565500 Rh2CG535300 Rh2DG574400 Rh4AG133100 Rh4AG133200 Rh4AG133300 Rh4BG128200 Rh4CG141000 Rh4DG127700 Rh4DG127900
rosa_wichuraiana Rw2G045540 Rw2G045650 Rw4G010790 Rw4G010800 Rw4G010810 Rw4G010830 Rw7G027620 Rw7G027630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 732
AarI CACCTGC 1 cut(s) 128
AatII GACGTC 1 cut(s) 1043
Acc36I ACCTGC 1 cut(s) 128
AccB7I CCANNNNNTGG 1 cut(s) 858
AccBSI CCGCTC 1 cut(s) 1178
AccIII TCCGGA 1 cut(s) 601
AciI CCGC 3 cut(s) 405, 660, 1178
AclWI GGATC 2 cut(s) 560, 573
AcsI RAATTY 3 cut(s) 847, 1346, 1485
AcuI CTGAAG 1 cut(s) 1458
AcyI GRCGYC 1 cut(s) 1040
AfaI GTAC 4 cut(s) 206, 384, 1162, 1658
AfiI CCNNNNNNNGG 5 cut(s) 240, 575, 858, 1397, 1518
AflIII ACRYGT 2 cut(s) 324, 1204
AgeI ACCGGT 1 cut(s) 262
AjnI CCWGG 3 cut(s) 169, 493, 574
Alw21I GWGCWC 1 cut(s) 1067
Alw26I GTCTC 4 cut(s) 136, 879, 1243, 1604
Alw44I GTGCAC 1 cut(s) 1063
AlwI GGATC 2 cut(s) 560, 573
AlwNI CAGNNNCTG 1 cut(s) 1187
Ama87I CYCGRG 2 cut(s) 59, 112
Aor13HI TCCGGA 1 cut(s) 601
AoxI GGCC 2 cut(s) 1468, 1537
ApaLI GTGCAC 1 cut(s) 1063
ApeKI GCWGC 5 cut(s) 80, 1083, 1086, 1181, 1418
ApoI RAATTY 3 cut(s) 847, 1346, 1485
AsiGI ACCGGT 1 cut(s) 262
AspS9I GGNCC 3 cut(s) 654, 662, 1469
AsuC2I CCSGG 1 cut(s) 338
AsuHPI GGTGA 1 cut(s) 1163
AsuNHI GCTAGC 1 cut(s) 595
AvaI CYCGRG 2 cut(s) 59, 112
AvaII GGWCC 2 cut(s) 654, 662
AxyI CCTNAGG 1 cut(s) 817
BaeGI GKGCMC 1 cut(s) 1067
BamHI GGATCC 1 cut(s) 565
BauI CACGAG 1 cut(s) 1547
Bbv12I GWGCWC 1 cut(s) 1067
BbvI GCAGC 5 cut(s) 92, 1095, 1098, 1193, 1405
BccI CCATC 5 cut(s) 98, 296, 302, 835, 1148
BceAI ACGGC 1 cut(s) 1201
BciT130I CCWGG 3 cut(s) 171, 495, 576
BclI TGATCA 1 cut(s) 469
BcnI CCSGG 1 cut(s) 338
BcoDI GTCTC 4 cut(s) 136, 879, 1243, 1604
BfaI CTAG 5 cut(s) 155, 386, 432, 596, 642
BfmI CTRYAG 2 cut(s) 135, 1410
BfuAI ACCTGC 1 cut(s) 128
BglI GCCNNNNNGGC 2 cut(s) 457, 979
BisI GCNGC 6 cut(s) 81, 1084, 1087, 1179, 1182, 1419
BlsI GCNGC 6 cut(s) 82, 1085, 1088, 1180, 1183, 1420
BmcAI AGTACT 1 cut(s) 1162
Bme1390I CCNGG 4 cut(s) 171, 338, 495, 576
Bme18I GGWCC 2 cut(s) 654, 662
BmeT110I CYCGRG 2 cut(s) 59, 112
BmgT120I GGNCC 3 cut(s) 654, 662, 1469
BmiI GGNNCC 1 cut(s) 567
BmrFI CCNGG 4 cut(s) 171, 338, 495, 576
BmsI GCATC 2 cut(s) 230, 1369
BmtI GCTAGC 1 cut(s) 599
BpmI CTGGAG 2 cut(s) 581, 904
BpuMI CCSGG 1 cut(s) 338
BsaBI GATNNNNATC 1 cut(s) 1646
BsaHI GRCGYC 1 cut(s) 1040
BsaI GGTCTC 1 cut(s) 879
BsaJI CCNNGG 2 cut(s) 665, 1540
BsaWI WCCGGW 2 cut(s) 262, 601
Bsc4I CCNNNNNNNGG 5 cut(s) 240, 575, 858, 1397, 1518
Bse118I RCCGGY 3 cut(s) 262, 425, 978
Bse1I ACTGG 6 cut(s) 369, 921, 1032, 1060, 1255, 1596
Bse21I CCTNAGG 1 cut(s) 817
Bse3DI GCAATG 1 cut(s) 1096
Bse8I GATNNNNATC 1 cut(s) 1646
BseAI TCCGGA 1 cut(s) 601
BseBI CCWGG 3 cut(s) 171, 495, 576
BseDI CCNNGG 2 cut(s) 665, 1540
BseGI GGATG 7 cut(s) 150, 472, 610, 846, 935, 1022, 1381
BseJI GATNNNNATC 1 cut(s) 1646
BseLI CCNNNNNNNGG 5 cut(s) 240, 575, 858, 1397, 1518
BseMI GCAATG 1 cut(s) 1096
BseMII CTCAG 3 cut(s) 216, 831, 1323
BseNI ACTGG 6 cut(s) 369, 921, 1032, 1060, 1255, 1596
BseRI GAGGAG 1 cut(s) 1565
BseSI GKGCMC 1 cut(s) 1067
BseXI GCAGC 5 cut(s) 92, 1095, 1098, 1193, 1405
BsgI GTGCAG 1 cut(s) 555
BshFI GGCC 2 cut(s) 1470, 1539
BshTI ACCGGT 1 cut(s) 262
BsiHKAI GWGCWC 1 cut(s) 1067
BsiHKCI CYCGRG 2 cut(s) 59, 112
BsiSI CCGG 5 cut(s) 263, 338, 426, 602, 979
BslFI GGGAC 3 cut(s) 49, 1007, 1204
BslI CCNNNNNNNGG 5 cut(s) 240, 575, 858, 1397, 1518
BsmAI GTCTC 4 cut(s) 136, 879, 1243, 1604
BsmFI GGGAC 3 cut(s) 49, 1007, 1204
BsmI GAATGC 1 cut(s) 1061
BsnI GGCC 2 cut(s) 1470, 1539
Bso31I GGTCTC 1 cut(s) 879
BsoBI CYCGRG 2 cut(s) 59, 112
Bsp1286I GDGCHC 1 cut(s) 1067
Bsp13I TCCGGA 1 cut(s) 601
Bsp143I GATC 6 cut(s) 118, 469, 565, 715, 1392, 1647
Bsp19I CCATGG 1 cut(s) 665
BspACI CCGC 3 cut(s) 405, 660, 1178
BspANI GGCC 2 cut(s) 1470, 1539
BspCNI CTCAG 3 cut(s) 217, 830, 1324
BspEI TCCGGA 1 cut(s) 601
BspHI TCATGA 1 cut(s) 1557
BspLI GGNNCC 1 cut(s) 567
BspMAI CTGCAG 1 cut(s) 139
BspMI ACCTGC 1 cut(s) 128
BspOI GCTAGC 1 cut(s) 599
BspPI GGATC 2 cut(s) 560, 573
BspTNI GGTCTC 1 cut(s) 879
BsrBI CCGCTC 1 cut(s) 1178
BsrDI GCAATG 1 cut(s) 1096
BsrFI RCCGGY 3 cut(s) 262, 425, 978
BsrI ACTGG 6 cut(s) 369, 921, 1032, 1060, 1255, 1596
BssAI RCCGGY 3 cut(s) 262, 425, 978
BssECI CCNNGG 2 cut(s) 665, 1540
BssMI GATC 6 cut(s) 118, 469, 565, 715, 1392, 1647
BssNI GRCGYC 1 cut(s) 1040
BssSI CACGAG 1 cut(s) 1547
BssT1I CCWWGG 2 cut(s) 665, 1540
Bst2BI CACGAG 1 cut(s) 1547
Bst2UI CCWGG 3 cut(s) 171, 495, 576
Bst4CI ACNGT 3 cut(s) 235, 648, 888
Bst6I CTCTTC 1 cut(s) 548
BstACI GRCGYC 1 cut(s) 1040
BstC8I GCNNGC 2 cut(s) 427, 597
BstDEI CTNAG 5 cut(s) 225, 298, 817, 1332, 1675
BstDSI CCRYGG 1 cut(s) 665
BstENI CCTNNNNNAGG 1 cut(s) 573
BstF5I GGATG 7 cut(s) 150, 472, 610, 846, 935, 1022, 1381
BstKTI GATC 6 cut(s) 121, 472, 568, 718, 1395, 1650
BstMAI GTCTC 4 cut(s) 136, 879, 1243, 1604
BstMBI GATC 6 cut(s) 118, 469, 565, 715, 1392, 1647
BstMWI GCNNNNNNNGC 3 cut(s) 457, 979, 1415
BstNI CCWGG 3 cut(s) 171, 495, 576
BstNSI RCATGY 4 cut(s) 202, 328, 1098, 1663
BstSCI CCNGG 4 cut(s) 169, 336, 493, 574
BstSFI CTRYAG 2 cut(s) 135, 1410
BstSLI GKGCMC 1 cut(s) 1067
BstV1I GCAGC 5 cut(s) 92, 1095, 1098, 1193, 1405
BstX2I RGATCY 1 cut(s) 565
BstXI CCANNNNNNTGG 1 cut(s) 217
BstYI RGATCY 1 cut(s) 565
Bsu36I CCTNAGG 1 cut(s) 817
BsuRI GGCC 2 cut(s) 1470, 1539
BtgI CCRYGG 1 cut(s) 665
BtsCI GGATG 7 cut(s) 150, 472, 610, 846, 935, 1022, 1381
BtsI GCAGTG 2 cut(s) 863, 1065
BtsIMutI CAGTG 7 cut(s) 100, 863, 893, 1039, 1065, 1067, 1248
BveI ACCTGC 1 cut(s) 128
Cac8I GCNNGC 2 cut(s) 427, 597
CaiI CAGNNNCTG 1 cut(s) 1187
CciI TCATGA 1 cut(s) 1557
Cfr10I RCCGGY 3 cut(s) 262, 425, 978
Cfr13I GGNCC 3 cut(s) 654, 662, 1469
CsiI ACCWGGT 2 cut(s) 169, 574
Csp6I GTAC 4 cut(s) 205, 383, 1161, 1657
CspAI ACCGGT 1 cut(s) 262
CviQI GTAC 4 cut(s) 205, 383, 1161, 1657
DdeI CTNAG 5 cut(s) 225, 298, 817, 1332, 1675
DpnI GATC 6 cut(s) 120, 471, 567, 717, 1394, 1649
DpnII GATC 6 cut(s) 118, 469, 565, 715, 1392, 1647
DraI TTTAAA 1 cut(s) 357
Eam1104I CTCTTC 1 cut(s) 548
EarI CTCTTC 1 cut(s) 548
Eco130I CCWWGG 2 cut(s) 665, 1540
Eco31I GGTCTC 1 cut(s) 879
Eco47I GGWCC 2 cut(s) 654, 662
Eco57I CTGAAG 1 cut(s) 1458
Eco81I CCTNAGG 1 cut(s) 817
Eco88I CYCGRG 2 cut(s) 59, 112
EcoNI CCTNNNNNAGG 1 cut(s) 573
EcoRI GAATTC 2 cut(s) 847, 1485
EcoRII CCWGG 3 cut(s) 169, 493, 574
EcoT14I CCWWGG 2 cut(s) 665, 1540
EcoT22I ATGCAT 1 cut(s) 1096
ErhI CCWWGG 2 cut(s) 665, 1540
FaqI GGGAC 3 cut(s) 49, 1007, 1204
FbaI TGATCA 1 cut(s) 469
Fnu4HI GCNGC 6 cut(s) 81, 1084, 1087, 1179, 1182, 1419
FokI GGATG 7 cut(s) 157, 459, 617, 853, 922, 1029, 1388
Fsp4HI GCNGC 6 cut(s) 81, 1084, 1087, 1179, 1182, 1419
FspBI CTAG 5 cut(s) 155, 386, 432, 596, 642
GluI GCNGC 6 cut(s) 81, 1084, 1087, 1179, 1182, 1419
GsuI CTGGAG 2 cut(s) 581, 904
HaeIII GGCC 2 cut(s) 1470, 1539
HapII CCGG 5 cut(s) 263, 338, 426, 602, 979
Hin1I GRCGYC 1 cut(s) 1040
HincII GTYRAC 2 cut(s) 291, 348
HindII GTYRAC 2 cut(s) 291, 348
HindIII AAGCTT 1 cut(s) 1703
HinfI GANTC 7 cut(s) 62, 510, 526, 538, 556, 826, 1123
HpaI GTTAAC 1 cut(s) 291
HpaII CCGG 5 cut(s) 263, 338, 426, 602, 979
HphI GGTGA 1 cut(s) 1163
Hpy166II GTNNAC 5 cut(s) 291, 348, 503, 1065, 1477
Hpy188I TCNGA 6 cut(s) 226, 271, 448, 1175, 1189, 1671
Hpy8I GTNNAC 5 cut(s) 291, 348, 503, 1065, 1477
HpyAV CCTTC 2 cut(s) 955, 1142
HpyCH4III ACNGT 3 cut(s) 235, 648, 888
HpyCH4IV ACGT 4 cut(s) 693, 1040, 1170, 1204
HpyF10VI GCNNNNNNNGC 3 cut(s) 457, 979, 1415
HpyF3I CTNAG 5 cut(s) 225, 298, 817, 1332, 1675
HpySE526I ACGT 4 cut(s) 693, 1040, 1170, 1204
Hsp92I GRCGYC 1 cut(s) 1040
Kpn2I TCCGGA 1 cut(s) 601
KroI GCCGGC 1 cut(s) 425
KroNI GCCGGC 1 cut(s) 427
Ksp22I TGATCA 1 cut(s) 469
KspAI GTTAAC 1 cut(s) 291
Kzo9I GATC 6 cut(s) 118, 469, 565, 715, 1392, 1647
LmnI GCTCC 3 cut(s) 604, 1590, 1671
Lsp1109I GCAGC 5 cut(s) 92, 1095, 1098, 1193, 1405
LweI GCATC 2 cut(s) 230, 1369
MabI ACCWGGT 2 cut(s) 169, 574
MaeI CTAG 5 cut(s) 155, 386, 432, 596, 642
MaeII ACGT 4 cut(s) 693, 1040, 1170, 1204
MaeIII GTNAC 2 cut(s) 739, 1036
MalI GATC 6 cut(s) 120, 471, 567, 717, 1394, 1649
MbiI CCGCTC 1 cut(s) 1178
MboI GATC 6 cut(s) 118, 469, 565, 715, 1392, 1647
MboII GAAGA 9 cut(s) 154, 273, 565, 765, 1038, 1300, 1333, 1387, 1640
MflI RGATCY 1 cut(s) 565
MhlI GDGCHC 1 cut(s) 1067
MlyI GAGTC 2 cut(s) 71, 565
MmeI TCCRAC 3 cut(s) 500, 1336, 1612
Mph1103I ATGCAT 1 cut(s) 1096
MroI TCCGGA 1 cut(s) 601
MroNI GCCGGC 1 cut(s) 425
MseI TTAA 9 cut(s) 290, 356, 1049, 1212, 1256, 1403, 1515, 1568, 1717
MslI CAYNNNNRTG 1 cut(s) 1113
MspA1I CMGCKG 2 cut(s) 83, 660
MspI CCGG 5 cut(s) 263, 338, 426, 602, 979
MspR9I CCNGG 4 cut(s) 171, 338, 495, 576
Mva1269I GAATGC 1 cut(s) 1061
MvaI CCWGG 3 cut(s) 171, 495, 576
MwoI GCNNNNNNNGC 3 cut(s) 457, 979, 1415
NaeI GCCGGC 1 cut(s) 427
NciI CCSGG 1 cut(s) 338
NcoI CCATGG 1 cut(s) 665
NdeII GATC 6 cut(s) 118, 469, 565, 715, 1392, 1647
NgoMIV GCCGGC 1 cut(s) 425
NheI GCTAGC 1 cut(s) 595
NlaIV GGNNCC 1 cut(s) 567
NmeAIII GCCGAG 1 cut(s) 1431
NmuCI GTSAC 1 cut(s) 1036
NsiI ATGCAT 1 cut(s) 1096
NspI RCATGY 4 cut(s) 202, 328, 1098, 1663
PaeR7I CTCGAG 1 cut(s) 59
PagI TCATGA 1 cut(s) 1557
PaqCI CACCTGC 1 cut(s) 128
PciI ACATGT 1 cut(s) 324
PctI GAATGC 1 cut(s) 1061
PdiI GCCGGC 1 cut(s) 427
PfeI GAWTC 5 cut(s) 510, 526, 538, 826, 1123
PflMI CCANNNNNTGG 1 cut(s) 858
PinAI ACCGGT 1 cut(s) 262
PkrI GCNGC 6 cut(s) 82, 1085, 1088, 1180, 1183, 1420
PleI GAGTC 2 cut(s) 70, 564
PpsI GAGTC 2 cut(s) 70, 564
PscI ACATGT 1 cut(s) 324
PsiI TTATAA 1 cut(s) 732
Psp6I CCWGG 3 cut(s) 169, 493, 574
PspGI CCWGG 3 cut(s) 169, 493, 574
PspN4I GGNNCC 1 cut(s) 567
PspPI GGNCC 3 cut(s) 654, 662, 1469
PsrI GAACNNNNNNTAC 2 cut(s) 375, 407
PstI CTGCAG 1 cut(s) 139
PstNI CAGNNNCTG 1 cut(s) 1187
PsuI RGATCY 1 cut(s) 565
PvuII CAGCTG 1 cut(s) 83
RsaI GTAC 4 cut(s) 206, 384, 1162, 1658
RsaNI GTAC 4 cut(s) 205, 383, 1161, 1657
RseI CAYNNNNRTG 1 cut(s) 1113
SaqAI TTAA 9 cut(s) 290, 356, 1049, 1212, 1256, 1403, 1515, 1568, 1717
SatI GCNGC 6 cut(s) 81, 1084, 1087, 1179, 1182, 1419
Sau3AI GATC 6 cut(s) 118, 469, 565, 715, 1392, 1647
Sau96I GGNCC 3 cut(s) 654, 662, 1469
ScaI AGTACT 1 cut(s) 1162
SchI GAGTC 2 cut(s) 71, 565
ScrFI CCNGG 4 cut(s) 171, 338, 495, 576
SduI GDGCHC 1 cut(s) 1067
SexAI ACCWGGT 2 cut(s) 169, 574
SfaNI GCATC 2 cut(s) 230, 1369
SfcI CTRYAG 2 cut(s) 135, 1410
Sfr274I CTCGAG 1 cut(s) 59
SinI GGWCC 2 cut(s) 654, 662
SlaI CTCGAG 1 cut(s) 59
SmiMI CAYNNNNRTG 1 cut(s) 1113
SmlI CTYRAG 1 cut(s) 59
SmoI CTYRAG 1 cut(s) 59
SsiI CCGC 3 cut(s) 405, 660, 1178
SspMI CTAG 5 cut(s) 155, 386, 432, 596, 642
StyD4I CCNGG 4 cut(s) 169, 336, 493, 574
StyI CCWWGG 2 cut(s) 665, 1540
TaaI ACNGT 3 cut(s) 235, 648, 888
TaiI ACGT 4 cut(s) 696, 1043, 1173, 1207
TaqI TCGA 2 cut(s) 60, 806
TatI WGTACW 3 cut(s) 382, 1160, 1656
TauI GCSGC 1 cut(s) 1181
TfiI GAWTC 5 cut(s) 510, 526, 538, 826, 1123
Tru1I TTAA 9 cut(s) 290, 356, 1049, 1212, 1256, 1403, 1515, 1568, 1717
Tru9I TTAA 9 cut(s) 290, 356, 1049, 1212, 1256, 1403, 1515, 1568, 1717
TscAI CASTG 7 cut(s) 100, 863, 893, 1039, 1067, 1072, 1255
TseFI GTSAC 1 cut(s) 1036
TseI GCWGC 5 cut(s) 80, 1083, 1086, 1181, 1418
Tsp45I GTSAC 1 cut(s) 1036
TspGWI ACGGA 1 cut(s) 274
TspRI CASTG 7 cut(s) 100, 863, 893, 1039, 1067, 1072, 1255
Van91I CCANNNNNTGG 1 cut(s) 858
VneI GTGCAC 1 cut(s) 1063
VpaK11BI GGWCC 2 cut(s) 654, 662
XagI CCTNNNNNAGG 1 cut(s) 573
XapI RAATTY 3 cut(s) 847, 1346, 1485
XbaI TCTAGA 1 cut(s) 641
XceI RCATGY 4 cut(s) 202, 328, 1098, 1663
XhoI CTCGAG 1 cut(s) 59
XspI CTAG 5 cut(s) 155, 386, 432, 596, 642
ZraI GACGTC 1 cut(s) 1041
ZrmI AGTACT 1 cut(s) 1162
Zsp2I ATGCAT 1 cut(s) 1096
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.