Prupe.3G225200_v2.0.a1

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0000564
Physical Location & Seq
Reverse (-)
22446507 .. 22450290
3784 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G225200.1

Sequence Viewer

Length: 2457 bp
ATGGATACCAAAACCAACCCAATGTTGGCGTTTATAGTTGTTTTATTGTGCCTATTTCTCGAGTCCCGTATCTCCTTTGCAGCTGACACCATCACTGCAAACCAATCTCTCTCCGGTGACCAGACAGTCGTCTCCGGGGATTTTGAACTGGGGTTCTTCGAATTAGATGGCCGCCACTACATAGGTACCTGGTATTCCCGCCGAGTGGTATCTGCGAATACCATAGTTTGGGTGGCAAATAGGGAAAAACCTATCTCTGATAGATTTTCAGCGGTGTTGAAGATCAAAGATGGCAATGTAGTTCTCTTTAATGAGGCCAAAACTCCAGTTTGGTCCACACATTTGACCTCCACCACCACCTCTGCTTCTGTGCAAGCAGTTCTTTTAGATAGTGGAAATCTTGTCTTAAGAGCTCATTCTAGTTCATCTGAGTTTTTATGGCAAAGCTTTCATCATCCAGCCCACACATGGCTACCAGGAGCCAAGGTTGCATTCAACAATATTACCAATCAAACCCAAATTCTCACTTCATGGAAGAACTCACGGAATCCTGCACCAGGTCTTTACTCTCTTGAGGTAGACCCGAATGGAAGCAATTCATTTATCTTACTGTGGAATAGGTCTAAACAGTATTGGACCAGTGGATCATGGAATGCAAGTTCAAATATTTTCAGCTTGTCTCCTGAGATGCGGCTTAATTACGTCTACAACTACAGCTTTGTTTCAAATGAAAACGGAAGTTATTTCACCTATTCGCTTTATGATCCTAAGAAAGTATCTCCATTCATGATGTCAGTATCGGGGCAGATTCAGCAACTAATTTGGTTTACGCCTTCCAGGCCATCGAAAATATTTTGGTCTTTACCAAGGCAGCAATGTGCTGTTCATCTGATTTGCGGGGCATTCGGGAGCTGCAATGAAAAATCCGGGGTCTTGTGTAATTGTTTGATGGGTTTTAAGCAGAAGTCGCCACGGGATTGGGCTTTGCAGGATTACTCAGGTGGGTGTCAAAGAAAAACCAATTTGCAGTGTGGGAATTCTACTAGTGTTATTGGGACGAAATACCAGTTTCTAGAAATGCATAGCATGTCAATGTCTGAAAATGAACTGCATGTTAAGGTTGGGAGTGCTAAGAAATGTGAATCAATCTGCTTAAATAACTGCTCTTGCACTGCTTATGCTTATGAAAGCAGTAACGCATGTTCAATTTGGATTGGAGATCTCCTGGGTGTGCAAGAACTTGTGGCAGATGACGCTGGTGGAAGAACTTTATACATCAGACTTGCAGCTTCTGAGCTTATGCATCTTCAAAATGGCAAGGGTGATGCGGATAAGCGCCCCCTTATAATTGCAATGGTCTCAGCAGCAGCAGAGTTGCTTATGATAATTTTTTGCTATTTCCTATGGAAGAAAAGATTGGGGAAGCGAAGGACGCAGAGGAGGAAGTATGGTGCGACTAAAATAAATTATGGTGCTGGAAGTGGAAAGAATGACACAGAACTACCACTCTTCGGTTTAAAGAGTATATTAAATACAATAAACAACTTCTCTGAAGCTAATAAACTGGGAGAGGGAGGATTTGGCCCTGTTTATAAGGGGATTTTGCTTGAAAATCAAGAAGTAGCCATAAAAAGACTGTCAAAGAAGTCAGGGCAAGGACATGAGGAGTTCATGAATGAGTTAAATCTTATAGCCAAGCTCCACCATACCAGTCTTGTTAGGCTCTTAGGTTGCTGTATTGAGGAGGAGGAACTGATCTTGATCTATGAGTTCATGCACCATCGAAGTTTGGACAAACTTTTGTTTGATTCATCTGAAAATGCAGAATTAGATTGGGGGAAACGCTTTCGAATTATAGAGGGTATTGCTCAGGGAGTACTTTATATCCACAAGCACTCTAGATTGAAAATCATACACAGGGACCTGAAAGCAAGTAATGTTCTGTTGGATGGAGCAATGAACCCAAAAATATCAGACTTTGGGATGGCTAAGATTTTTGAGATAAATCAAACTGAAGCAAATACCAACAGGGTTGTTGGCACATACGGATACATGTCTCCAGAATATGCAAGATATGGCCATTTCTCTGAGAAACTAGATGTGTTTAGTTTTGGAGTGTTGTTGTTGGAGATTGTAAGTGGAAAGAGGAATGCTGCTTTTTATCACTTTGAACATTCACCAACTCTTGCTGGATGGTGTATCCATGTAGGGTTTTTGTGTGTTCAAGAAGCTCCAGCTGATCGACCAACAATGGCCTCTGTAATTCGTATGTTGCAGAGCAATGAAGCCACATCACTTCCACCCTCCAAGGAACCGGCCTTTTCAACTAACAGGAATTCCATACCTGTTGTTGGTTCTTCTCAATTACCTGCCGTTTTCCAACAATGGAGTCACCATCAGTTTGCCAGAAGGTCGATAGAGGTTCCAATATTTGTTTCCTTCCCTCAACACATTTAA

Protein Analysis

819

Amino Acids

91.68

Weight (kDa)

8.74

Isoelectric Point (pI)

44.5

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000564)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g44064 FvH4_6g44064 FvH4_6g44100 FvH4_6g44101 FvH4_6g51830
malus_domestica MD09G1094400.v1.1 MD09G1094800.v1.1 MD09G1095200.v1.1 MD09G1095900.v1.1 MD09G1096000.v1.1 MD09G1096200.v1.1 MD17G1083900.v1.1 MD17G1088500.v1.1
prunus_persica Prupe.3G224900_v2.0.a1 Prupe.3G224900_v2.0.a1 Prupe.3G224900_v2.0.a1 Prupe.3G224900_v2.0.a1 Prupe.3G224900_v2.0.a1 Prupe.3G224900_v2.0.a1 Prupe.3G225100_v2.0.a1 Prupe.3G225100_v2.0.a1 Prupe.3G225100_v2.0.a1 Prupe.3G225200_v2.0.a1 Prupe.3G225200_v2.0.a1
pyrus_communis pycom09g01920 pycom09g01930 pycom09g01940 pycom09g01950 pycom09g02000 pycom09g02050 pycom09g02070 pycom17g08540
rosa_chinensis RchiOBHm_Chr1g0318601 RchiOBHm_Chr1g0318761 RchiOBHm_Chr1g0342541 RchiOBHm_Chr2g0160651 RchiOBHm_Chr2g0160831 RchiOBHm_Chr4g0405661 RchiOBHm_Chr4g0405691 RchiOBHm_Chr4g0405701 RchiOBHm_Chr7g0238591
rosa_laevigata RLG00000002292 RLG00000008826 RLG00000008828 RLG00000008829 RLG00000021243 RLG00000021245 RLG00000030522
rosa_multiflora Rmu_co8432573.1_g000001 Rmu_sc0001200.1_g000041 Rmu_sc0001200.1_g000043 Rmu_sc0002545.1_g000017 Rmu_sc0002773.1_g000017 Rmu_sc0012550.1_g000008
rosa_roxburghii Rroxscaffold_2G00089500 Rroxscaffold_5G00350130 Rroxscaffold_5G00350180
rosa_rugosa Rorug01G0022300 Rorug04G0061600
rosa_samantha Rh1AG035300 Rh1CG034300 Rh1DG036100 Rh1DG149100 Rh2AG550900 Rh2BG564200 Rh2BG565500 Rh2CG535300 Rh2DG574400 Rh4AG133100 Rh4AG133200 Rh4AG133300 Rh4BG128200 Rh4CG141000 Rh4DG127700 Rh4DG127900
rosa_wichuraiana Rw2G045540 Rw2G045650 Rw4G010790 Rw4G010800 Rw4G010810 Rw4G010830 Rw7G027620 Rw7G027630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1346, 1593
AasI GACNNNNNNGTC 1 cut(s) 125
Acc36I ACCTGC 1 cut(s) 2377
Acc65I GGTACC 1 cut(s) 185
AccB1I GGYRCC 1 cut(s) 185
AccB7I CCANNNNNTGG 1 cut(s) 228
AccI GTMKAC 2 cut(s) 579, 705
AciI CCGC 6 cut(s) 172, 199, 272, 691, 897, 1328
AclWI GGATC 2 cut(s) 652, 758
AcoI YGGCCR 2 cut(s) 169, 2077
AcsI RAATTY 3 cut(s) 519, 1036, 2335
AcuI CTGAAG 2 cut(s) 1572, 2034
AfaI GTAC 2 cut(s) 187, 1878
AfiI CCNNNNNNNGG 7 cut(s) 25, 205, 228, 468, 557, 1511, 2351
AflII CTTAAG 1 cut(s) 406
AflIII ACRYGT 1 cut(s) 2052
AhlI ACTAGT 1 cut(s) 1043
AjnI CCWGG 5 cut(s) 188, 475, 556, 836, 1224
AjuI GAANNNNNNNTTGG 6 cut(s) 476, 508, 1400, 1432, 2419, 2451
AloI GAACNNNNNNTCC 2 cut(s) 643, 675
Alw21I GWGCWC 1 cut(s) 415
Alw26I GTCTC 4 cut(s) 136, 684, 1363, 2061
AlwI GGATC 2 cut(s) 652, 758
AlwNI CAGNNNCTG 1 cut(s) 1292
Ama87I CYCGRG 1 cut(s) 59
AoxI GGCC 7 cut(s) 169, 315, 839, 1582, 2077, 2253, 2316
ApeKI GCWGC 7 cut(s) 80, 871, 912, 1286, 1364, 1367, 2153
ApoI RAATTY 3 cut(s) 519, 1036, 2335
Asp700I GAANNNNTTC 1 cut(s) 595
Asp718I GGTACC 1 cut(s) 185
AspLEI GCGC 1 cut(s) 1338
AspS9I GGNCC 4 cut(s) 333, 636, 1583, 1921
AsuC2I CCSGG 2 cut(s) 136, 928
AsuHPI GGTGA 5 cut(s) 128, 739, 1334, 2169, 2384
AsuII TTCGAA 2 cut(s) 159, 1849
AvaI CYCGRG 1 cut(s) 59
AvaII GGWCC 3 cut(s) 333, 636, 1921
BaeI ACNNNNGTAYC 2 cut(s) 169, 202
BalI TGGCCA 1 cut(s) 2079
BanI GGYRCC 1 cut(s) 185
BanII GRGCYC 1 cut(s) 415
Bbv12I GWGCWC 1 cut(s) 415
BbvI GCAGC 7 cut(s) 92, 883, 899, 1298, 1376, 1379, 2140
BceAI ACGGC 1 cut(s) 2357
BciT130I CCWGG 5 cut(s) 190, 477, 558, 838, 1226
BciVI GTATCC 2 cut(s) 2042, 2210
BcnI CCSGG 2 cut(s) 136, 928
BcoDI GTCTC 4 cut(s) 136, 684, 1363, 2061
BcuI ACTAGT 1 cut(s) 1043
BfaI CTAG 5 cut(s) 420, 1044, 1073, 1899, 2096
BfmI CTRYAG 1 cut(s) 712
BfoI RGCGCY 1 cut(s) 1339
BfrI CTTAAG 1 cut(s) 406
BfuAI ACCTGC 1 cut(s) 2377
BfuI GTATCC 2 cut(s) 2042, 2210
BglI GCCNNNNNGGC 1 cut(s) 838
BglII AGATCT 1 cut(s) 1219
BisI GCNGC 9 cut(s) 81, 172, 692, 872, 913, 1287, 1365, 1368, 2154
BlsI GCNGC 9 cut(s) 82, 173, 693, 873, 914, 1288, 1366, 1369, 2155
BmcAI AGTACT 1 cut(s) 1878
Bme1390I CCNGG 7 cut(s) 136, 190, 477, 558, 838, 928, 1226
Bme18I GGWCC 3 cut(s) 333, 636, 1921
BmeT110I CYCGRG 1 cut(s) 59
BmgT120I GGNCC 4 cut(s) 333, 636, 1583, 1921
BmiI GGNNCC 5 cut(s) 187, 481, 1922, 2313, 2424
BmrFI CCNGG 7 cut(s) 136, 190, 477, 558, 838, 928, 1226
BmrI ACTGGG 2 cut(s) 158, 1574
BmsI GCATC 3 cut(s) 678, 1312, 1315
BmuI ACTGGG 2 cut(s) 158, 1574
BoxI GACNNNNGTC 1 cut(s) 128
BpmI CTGGAG 3 cut(s) 309, 2043, 2217
Bpu10I CCTNAGC 1 cut(s) 1869
Bpu14I TTCGAA 2 cut(s) 159, 1849
BpuEI CTTGAG 1 cut(s) 593
BpuMI CCSGG 2 cut(s) 136, 928
BsaBI GATNNNNATC 1 cut(s) 1760
BsaI GGTCTC 1 cut(s) 1363
BsaJI CCNNGG 7 cut(s) 135, 483, 866, 927, 971, 1225, 2307
BsaWI WCCGGW 1 cut(s) 113
BsaXI ACNNNNNCTCC 2 cut(s) 471, 501
Bsc4I CCNNNNNNNGG 7 cut(s) 25, 205, 228, 468, 557, 1511, 2351
Bse118I RCCGGY 1 cut(s) 2314
Bse1I ACTGG 6 cut(s) 153, 326, 639, 1066, 1569, 1710
Bse3DI GCAATG 6 cut(s) 301, 881, 922, 1359, 1962, 2287
Bse8I GATNNNNATC 1 cut(s) 1760
BseBI CCWGG 5 cut(s) 190, 477, 558, 838, 1226
BseDI CCNNGG 7 cut(s) 135, 483, 866, 927, 971, 1225, 2307
BseGI GGATG 4 cut(s) 454, 1954, 1989, 2198
BseJI GATNNNNATC 1 cut(s) 1760
BseLI CCNNNNNNNGG 7 cut(s) 25, 205, 228, 468, 557, 1511, 2351
BseMI GCAATG 6 cut(s) 301, 881, 922, 1359, 1962, 2287
BseMII CTCAG 7 cut(s) 420, 675, 1011, 1284, 1374, 1883, 2079
BseNI ACTGG 6 cut(s) 153, 326, 639, 1066, 1569, 1710
BseRI GAGGAG 4 cut(s) 1453, 1679, 1757, 1760
BseXI GCAGC 7 cut(s) 92, 883, 899, 1298, 1376, 1379, 2140
BsgI GTGCAG 1 cut(s) 537
BshFI GGCC 7 cut(s) 171, 317, 841, 1584, 2079, 2255, 2318
BshNI GGYRCC 1 cut(s) 185
BsiHKAI GWGCWC 1 cut(s) 415
BsiHKCI CYCGRG 1 cut(s) 59
BsiSI CCGG 4 cut(s) 114, 135, 927, 2315
BslFI GGGAC 3 cut(s) 49, 1069, 1934
BslI CCNNNNNNNGG 7 cut(s) 25, 205, 228, 468, 557, 1511, 2351
BsmAI GTCTC 4 cut(s) 136, 684, 1363, 2061
BsmBI CGTCTC 1 cut(s) 136
BsmFI GGGAC 3 cut(s) 49, 1069, 1934
BsmI GAATGC 4 cut(s) 491, 658, 902, 2155
BsnI GGCC 7 cut(s) 171, 317, 841, 1584, 2079, 2255, 2318
Bso31I GGTCTC 1 cut(s) 1363
BsoBI CYCGRG 1 cut(s) 59
Bsp119I TTCGAA 2 cut(s) 159, 1849
Bsp1286I GDGCHC 1 cut(s) 415
Bsp143I GATC 7 cut(s) 282, 644, 763, 1219, 1755, 1761, 2239
BspACI CCGC 6 cut(s) 172, 199, 272, 691, 897, 1328
BspANI GGCC 7 cut(s) 171, 317, 841, 1584, 2079, 2255, 2318
BspCNI CTCAG 7 cut(s) 421, 676, 1010, 1285, 1373, 1882, 2080
BspHI TCATGA 2 cut(s) 786, 1671
BspLI GGNNCC 5 cut(s) 187, 481, 1922, 2313, 2424
BspMI ACCTGC 1 cut(s) 2377
BspPI GGATC 2 cut(s) 652, 758
BspT104I TTCGAA 2 cut(s) 159, 1849
BspT107I GGYRCC 1 cut(s) 185
BspTI CTTAAG 1 cut(s) 406
BspTNI GGTCTC 1 cut(s) 1363
BsrDI GCAATG 6 cut(s) 301, 881, 922, 1359, 1962, 2287
BsrFI RCCGGY 1 cut(s) 2314
BsrI ACTGG 6 cut(s) 153, 326, 639, 1066, 1569, 1710
BssAI RCCGGY 1 cut(s) 2314
BssECI CCNNGG 7 cut(s) 135, 483, 866, 927, 971, 1225, 2307
BssMI GATC 7 cut(s) 282, 644, 763, 1219, 1755, 1761, 2239
BssT1I CCWWGG 3 cut(s) 483, 866, 2307
Bst2UI CCWGG 5 cut(s) 190, 477, 558, 838, 1226
Bst4CI ACNGT 4 cut(s) 127, 612, 630, 1638
Bst6I CTCTTC 1 cut(s) 1514
BstAFI CTTAAG 1 cut(s) 406
BstBI TTCGAA 2 cut(s) 159, 1849
BstC8I GCNNGC 1 cut(s) 375
BstDSI CCRYGG 1 cut(s) 971
BstEII GGTNACC 1 cut(s) 116
BstENI CCTNNNNNAGG 1 cut(s) 555
BstF5I GGATG 4 cut(s) 454, 1954, 1989, 2198
BstH2I RGCGCY 1 cut(s) 1339
BstHHI GCGC 1 cut(s) 1338
BstKTI GATC 7 cut(s) 285, 647, 766, 1222, 1758, 1764, 2242
BstMAI GTCTC 4 cut(s) 136, 684, 1363, 2061
BstMBI GATC 7 cut(s) 282, 644, 763, 1219, 1755, 1761, 2239
BstMWI GCNNNNNNNGC 6 cut(s) 488, 811, 838, 967, 1253, 1432
BstNI CCWGG 5 cut(s) 190, 477, 558, 838, 1226
BstNSI RCATGY 4 cut(s) 1090, 1115, 1203, 2056
BstPAI GACNNNNGTC 1 cut(s) 128
BstPI GGTNACC 1 cut(s) 116
BstSCI CCNGG 7 cut(s) 134, 188, 475, 556, 836, 926, 1224
BstSFI CTRYAG 1 cut(s) 712
BstV1I GCAGC 7 cut(s) 92, 883, 899, 1298, 1376, 1379, 2140
BstX2I RGATCY 1 cut(s) 1219
BstXI CCANNNNNNTGG 1 cut(s) 978
BstYI RGATCY 1 cut(s) 1219
BsuI GTATCC 2 cut(s) 2042, 2210
BsuRI GGCC 7 cut(s) 171, 317, 841, 1584, 2079, 2255, 2318
BtgI CCRYGG 1 cut(s) 971
BtsCI GGATG 4 cut(s) 454, 1954, 1989, 2198
BtsI GCAGTG 3 cut(s) 93, 1034, 1170
BtsIMutI CAGTG 4 cut(s) 93, 646, 1034, 1170
BveI ACCTGC 1 cut(s) 2377
Cac8I GCNNGC 1 cut(s) 375
CaiI CAGNNNCTG 1 cut(s) 1292
CciI TCATGA 2 cut(s) 786, 1671
CfoI GCGC 1 cut(s) 1338
Cfr10I RCCGGY 1 cut(s) 2314
Cfr13I GGNCC 4 cut(s) 333, 636, 1583, 1921
CseI GACGC 2 cut(s) 1262, 1441
CsiI ACCWGGT 2 cut(s) 188, 556
Csp6I GTAC 2 cut(s) 186, 1877
CviQI GTAC 2 cut(s) 186, 1877
DpnI GATC 7 cut(s) 284, 646, 765, 1221, 1757, 1763, 2241
DpnII GATC 7 cut(s) 282, 644, 763, 1219, 1755, 1761, 2239
DraI TTTAAA 1 cut(s) 1518
DrdI GACNNNNNNGTC 1 cut(s) 125
DseDI GACNNNNNNGTC 1 cut(s) 125
EaeI YGGCCR 2 cut(s) 169, 2077
Eam1104I CTCTTC 1 cut(s) 1514
EarI CTCTTC 1 cut(s) 1514
Ecl136II GAGCTC 1 cut(s) 413
Eco130I CCWWGG 3 cut(s) 483, 866, 2307
Eco24I GRGCYC 1 cut(s) 415
Eco31I GGTCTC 1 cut(s) 1363
Eco47I GGWCC 3 cut(s) 333, 636, 1921
Eco53kI GAGCTC 1 cut(s) 413
Eco57I CTGAAG 2 cut(s) 1572, 2034
Eco88I CYCGRG 1 cut(s) 59
Eco91I GGTNACC 1 cut(s) 116
EcoICRI GAGCTC 1 cut(s) 413
EcoNI CCTNNNNNAGG 1 cut(s) 555
EcoO109I RGGNCCY 1 cut(s) 1921
EcoO65I GGTNACC 1 cut(s) 116
EcoRI GAATTC 2 cut(s) 1036, 2335
EcoRII CCWGG 5 cut(s) 188, 475, 556, 836, 1224
EcoT14I CCWWGG 3 cut(s) 483, 866, 2307
EcoT22I ATGCAT 2 cut(s) 1083, 1305
EcoT38I GRGCYC 1 cut(s) 415
ErhI CCWWGG 3 cut(s) 483, 866, 2307
Esp3I CGTCTC 1 cut(s) 136
FalI AAGNNNNNCTT 2 cut(s) 366, 398
FaqI GGGAC 3 cut(s) 49, 1069, 1934
FauI CCCGC 2 cut(s) 206, 890
FblI GTMKAC 2 cut(s) 579, 705
Fnu4HI GCNGC 9 cut(s) 81, 172, 692, 872, 913, 1287, 1365, 1368, 2154
FokI GGATG 4 cut(s) 441, 1961, 1996, 2205
FriOI GRGCYC 1 cut(s) 415
Fsp4HI GCNGC 9 cut(s) 81, 172, 692, 872, 913, 1287, 1365, 1368, 2154
FspBI CTAG 5 cut(s) 420, 1044, 1073, 1899, 2096
GlaI GCGC 1 cut(s) 1337
GluI GCNGC 9 cut(s) 81, 172, 692, 872, 913, 1287, 1365, 1368, 2154
GsuI CTGGAG 3 cut(s) 309, 2043, 2217
HaeII RGCGCY 1 cut(s) 1339
HaeIII GGCC 7 cut(s) 171, 317, 841, 1584, 2079, 2255, 2318
HapII CCGG 4 cut(s) 114, 135, 927, 2315
HgaI GACGC 2 cut(s) 1262, 1441
HhaI GCGC 1 cut(s) 1338
Hin6I GCGC 1 cut(s) 1336
HinP1I GCGC 1 cut(s) 1336
HindIII AAGCTT 1 cut(s) 445
HinfI GANTC 6 cut(s) 62, 547, 808, 1142, 1808, 2389
HpaII CCGG 4 cut(s) 114, 135, 927, 2315
HphI GGTGA 5 cut(s) 128, 739, 1334, 2169, 2384
Hpy166II GTNNAC 4 cut(s) 336, 580, 706, 828
Hpy8I GTNNAC 4 cut(s) 336, 580, 706, 828
HpyAV CCTTC 4 cut(s) 843, 1422, 2403, 2449
HpyCH4III ACNGT 4 cut(s) 127, 612, 630, 1638
HpyCH4IV ACGT 1 cut(s) 702
HpyF10VI GCNNNNNNNGC 6 cut(s) 488, 811, 838, 967, 1253, 1432
HpySE526I ACGT 1 cut(s) 702
HspAI GCGC 1 cut(s) 1336
KpnI GGTACC 1 cut(s) 189
Kzo9I GATC 7 cut(s) 282, 644, 763, 1219, 1755, 1761, 2239
LmnI GCTCC 5 cut(s) 479, 909, 1704, 1952, 2236
Lsp1109I GCAGC 7 cut(s) 92, 883, 899, 1298, 1376, 1379, 2140
LweI GCATC 3 cut(s) 678, 1312, 1315
MabI ACCWGGT 2 cut(s) 188, 556
MaeI CTAG 5 cut(s) 420, 1044, 1073, 1899, 2096
MaeII ACGT 1 cut(s) 702
MaeIII GTNAC 3 cut(s) 116, 1193, 2390
MalI GATC 7 cut(s) 284, 646, 765, 1221, 1757, 1763, 2241
MboI GATC 7 cut(s) 282, 644, 763, 1219, 1755, 1761, 2239
MboII GAAGA 8 cut(s) 148, 292, 547, 1275, 1298, 1420, 1501, 2349
MflI RGATCY 1 cut(s) 1219
MhlI GDGCHC 1 cut(s) 415
MlsI TGGCCA 1 cut(s) 2079
MluNI TGGCCA 1 cut(s) 2079
MlyI GAGTC 2 cut(s) 71, 2398
MmeI TCCRAC 3 cut(s) 1926, 2106, 2404
Mox20I TGGCCA 1 cut(s) 2079
Mph1103I ATGCAT 2 cut(s) 1083, 1305
MroXI GAANNNNTTC 1 cut(s) 595
MscI TGGCCA 1 cut(s) 2079
MslI CAYNNNNRTG 1 cut(s) 1091
Msp20I TGGCCA 1 cut(s) 2079
MspA1I CMGCKG 3 cut(s) 83, 272, 2237
MspCI CTTAAG 1 cut(s) 406
MspI CCGG 4 cut(s) 114, 135, 927, 2315
MspR9I CCNGG 7 cut(s) 136, 190, 477, 558, 838, 928, 1226
Mva1269I GAATGC 4 cut(s) 491, 658, 902, 2155
MvaI CCWGG 5 cut(s) 190, 477, 558, 838, 1226
MwoI GCNNNNNNNGC 6 cut(s) 488, 811, 838, 967, 1253, 1432
NciI CCSGG 2 cut(s) 136, 928
NdeII GATC 7 cut(s) 282, 644, 763, 1219, 1755, 1761, 2239
NlaIV GGNNCC 5 cut(s) 187, 481, 1922, 2313, 2424
NmeAIII GCCGAG 1 cut(s) 227
NmuCI GTSAC 2 cut(s) 116, 2390
NsiI ATGCAT 2 cut(s) 1083, 1305
NspI RCATGY 4 cut(s) 1090, 1115, 1203, 2056
NspV TTCGAA 2 cut(s) 159, 1849
PaeR7I CTCGAG 1 cut(s) 59
PagI TCATGA 2 cut(s) 786, 1671
PciI ACATGT 1 cut(s) 2052
PctI GAATGC 4 cut(s) 491, 658, 902, 2155
PdmI GAANNNNTTC 1 cut(s) 595
PfeI GAWTC 4 cut(s) 547, 808, 1142, 1808
PflMI CCANNNNNTGG 1 cut(s) 228
PkrI GCNGC 9 cut(s) 82, 173, 693, 873, 914, 1288, 1366, 1369, 2155
PleI GAGTC 2 cut(s) 70, 2397
PpsI GAGTC 2 cut(s) 70, 2397
PpuMI RGGWCCY 1 cut(s) 1921
PscI ACATGT 1 cut(s) 2052
PshAI GACNNNNGTC 1 cut(s) 128
PsiI TTATAA 2 cut(s) 1346, 1593
Psp124BI GAGCTC 1 cut(s) 415
Psp5II RGGWCCY 1 cut(s) 1921
Psp6I CCWGG 5 cut(s) 188, 475, 556, 836, 1224
PspEI GGTNACC 1 cut(s) 116
PspGI CCWGG 5 cut(s) 188, 475, 556, 836, 1224
PspN4I GGNNCC 5 cut(s) 187, 481, 1922, 2313, 2424
PspPI GGNCC 4 cut(s) 333, 636, 1583, 1921
PspPPI RGGWCCY 1 cut(s) 1921
PsrI GAACNNNNNNTAC 2 cut(s) 1186, 1218
PstNI CAGNNNCTG 1 cut(s) 1292
PsuI RGATCY 1 cut(s) 1219
PvuII CAGCTG 2 cut(s) 83, 2237
RsaI GTAC 2 cut(s) 187, 1878
RsaNI GTAC 2 cut(s) 186, 1877
RseI CAYNNNNRTG 1 cut(s) 1091
SacI GAGCTC 1 cut(s) 415
SatI GCNGC 9 cut(s) 81, 172, 692, 872, 913, 1287, 1365, 1368, 2154
Sau3AI GATC 7 cut(s) 282, 644, 763, 1219, 1755, 1761, 2239
Sau96I GGNCC 4 cut(s) 333, 636, 1583, 1921
ScaI AGTACT 1 cut(s) 1878
SchI GAGTC 2 cut(s) 71, 2398
ScrFI CCNGG 7 cut(s) 136, 190, 477, 558, 838, 928, 1226
SduI GDGCHC 1 cut(s) 415
SexAI ACCWGGT 2 cut(s) 188, 556
SfaNI GCATC 3 cut(s) 678, 1312, 1315
SfcI CTRYAG 1 cut(s) 712
Sfr274I CTCGAG 1 cut(s) 59
SfuI TTCGAA 2 cut(s) 159, 1849
SinI GGWCC 3 cut(s) 333, 636, 1921
SlaI CTCGAG 1 cut(s) 59
SmiMI CAYNNNNRTG 1 cut(s) 1091
SmlI CTYRAG 3 cut(s) 59, 406, 572
SmoI CTYRAG 3 cut(s) 59, 406, 572
SpeI ACTAGT 1 cut(s) 1043
SsiI CCGC 6 cut(s) 172, 199, 272, 691, 897, 1328
SspI AATATT 4 cut(s) 502, 667, 852, 2430
SspMI CTAG 5 cut(s) 420, 1044, 1073, 1899, 2096
SstI GAGCTC 1 cut(s) 415
StyD4I CCNGG 7 cut(s) 134, 188, 475, 556, 836, 926, 1224
StyI CCWWGG 3 cut(s) 483, 866, 2307
TaaI ACNGT 4 cut(s) 127, 612, 630, 1638
TaiI ACGT 1 cut(s) 705
TaqI TCGA 7 cut(s) 60, 159, 845, 1783, 1849, 2242, 2414
TatI WGTACW 1 cut(s) 1876
TauI GCSGC 2 cut(s) 174, 694
TfiI GAWTC 4 cut(s) 547, 808, 1142, 1808
TscAI CASTG 4 cut(s) 100, 646, 1034, 1177
TseFI GTSAC 2 cut(s) 116, 2390
TseI GCWGC 7 cut(s) 80, 871, 912, 1286, 1364, 1367, 2153
Tsp45I GTSAC 2 cut(s) 116, 2390
TspGWI ACGGA 3 cut(s) 559, 750, 2061
TspRI CASTG 4 cut(s) 100, 646, 1034, 1177
Van91I CCANNNNNTGG 1 cut(s) 228
Vha464I CTTAAG 1 cut(s) 406
VpaK11BI GGWCC 3 cut(s) 333, 636, 1921
XagI CCTNNNNNAGG 1 cut(s) 555
XapI RAATTY 3 cut(s) 519, 1036, 2335
XbaI TCTAGA 2 cut(s) 1072, 1898
XceI RCATGY 4 cut(s) 1090, 1115, 1203, 2056
XcmI CCANNNNNNNNNTGG 3 cut(s) 22, 229, 465
XhoI CTCGAG 1 cut(s) 59
XmiI GTMKAC 2 cut(s) 579, 705
XmnI GAANNNNTTC 1 cut(s) 595
XspI CTAG 5 cut(s) 420, 1044, 1073, 1899, 2096
ZrmI AGTACT 1 cut(s) 1878
Zsp2I ATGCAT 2 cut(s) 1083, 1305
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.