MD09G1095200.v1.1

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0000564
Physical Location & Seq
Forward (+)
6895558 .. 6898723
3166 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1095200.v1.1.491

Sequence Viewer

Length: 2505 bp
ATGGATACCAAACCAAACTCAGAGTTCGTGTTCTCCATTATTTTATTATGTGTATTTCTCAAGTCCAATGTTTACCTAGCAGCTGACACTATCGGTGAAAACCAATCTCTATCTGGCCATCAAACCATTGTGTCTGCAGGTGGGATTTTTGAGTTAGGTTTCTTCAAACCTGGTCAGCTTTCAAACTACTATATAGGCATATGGTACTCCAAGCAAGTAGTATCTGAGATGACCGTTGTTTGGGTAGCAAATAGGGAAATACCAGTATCTGATAGATTTTCTTCGGTCTTGAGCATCTTAGATGGTAATTTAGTTCTCTTGAACCAGTCCAAAACCCTGGTTTGGTCAACAAATTTAAACTCCACCACCACTTCTGGTTCTTTACAAGCAGTTCTTTTGGATAGTGGAAACTTTGTCTTGAGAGCGGGGTCTAGTAACAATACATCAGAGCCTTTATGGCAAAGCTTTGATCATCCAACCCATACTTGGCTACCAAGAGGTAGACTTGGATTCAACAAAATTACAAATCATACCCAAACTCTCACTTCATGGAAGAATTCGGAAAATCCTGCACCAGGTCTTTACTCTCTTGAGCTAGCCCCGGACGAAAGCAGTTCATATATCTTATTGTGGAATAGGTCTAAACAGTATTGGATCAGTGCACCATGGAATGAAAGTTCACATATTTTCAAGGTGGCTCCTGAAAATCAAATGTTCAATTATAGTTATGTTACGAATGAGAACGAAAGGTATTTCACCTATTCTCTTTACGATCTAAAAACTGTATCTCGATGCTCCTTGTCTGTCTCGGGCCAGATTCAGCAACTAACATGGTTGGAGAAGCAGTGGACTTTGCTTTGGTCTCTACCATTACAACAGTGTGATGTTTATGCTTTTTGTGGGGCTTTCTCTAGTTGCAATCCCACATCCTTGGACAACTGCAAATGTTTGAAGGGCTTTGAGCCAAACCGGCAGAGTGATTGGGACTTGCAGATTTATTCTGGTGGGTGTTCAAGAAGAACCAGTATGCAGTGTGGGAATGCTGCTAGTGCGAATGGGACGGGTGATGGGTTATTGGGAGACGAGTTTCTAGAAATACATAGTATTTCATTGCCTGAAAATAACCAATATGGATACGCTCTTGGTATCGAAAGTTGTAGATCAGCCTGTTTAAATGAATGCCATTGCACTGCTTATGCTTATGAAAGCAACAGTTATTGTTCAATATGGCATGGAGATGTTTTTCTGCCAGAGCTTGTAGCAAGTGACGGTGGTGGAAGAACTTTATACATCAGAGCTGCAGCTTCTGACATTAAGAAAAAACGTTTAATTAAGCCGTCCCTTGTGATTGCAATTGTCACAACAGTCACAGGTTTGCTTCTTGTCATTTTTGGCTATTTCTTATGGAAGAAGACAGTGGGAAAGAAAAGAGAGCAAAGGAATAGATATGGTGAGACTAAAAGTAAATTTGCTGCTGGAGGTGAAAACAACGATGCAGAACTGCCGATCTTTGGTTTAAGGGCTATAATAGCTGCTACAAACAACTTCGCTGAAGCTAATAAACTCGGAGAGGGAGGATTTGGCCCTGTCTACAAGGGAATTTTGGCTGAAAATCAAGAAGTAGCCATAAAAAGGTTATCAAAAAAGTCAGGTCAAGGACAACAGGAGTTCATGAATGAGTTAAAACTTATTGCCAAGCTCCAACATACCAATCTCGTTAGGCTCTTGGGTTGCTGTCTTGAAGAGGAGGAAATGATATTGATCTACGAGTACATGCCCAATCGGAGCTTGGACAAACTTTTGTTTGATGCATCTGAAAAAAGAGAATTAGATTGGGGAAGACGTTTTCGAATTATAGAAGGCATTGCTCAAGGAGTACTTTATATCCACAAGTACTCTAGATTGAAAATCATCCACAGGGACCTAAAAGCAAGTAACATACTGTTGGATAGAGCCTTGAATCCCAAAATTTCAGACTTTGGAATGGCAAGGATTTTCGGGATAAACCAGACTGAAGCAAATACCAATAGGGTTGTTGGCACATACGGTTACATGTCACCTGAATATGCAAGATATGGTCATTTCTCTGAGAAATTAGATGTATTCAGCTTCGGAGTGTTATTGTTGGAGATTGTAAGCGGAAAGAAAAATGCTGCTTTTTATCGCTTTGAACATTCACCAACTCTTGCTGGATGGGCATGGGAGTTGTGGAAAGAAGGAAGAGGAATGGAGGTGATCGATGAATCGGTAAGGGAAACGTGCCACCTCGATGAAGCTTTGAAGTGTATCCATGTAGGGTTTTTGTGTGTTCAAGAAGCTCCAGCTGATCGACCAACAATGTCTTCGGTAATTCATATGTTGCAGGGCAATGAATCTACGTCTCTTCCACCTTCCAAAGAACCTACCTTTTCAACACACAGACATTCCAATCCTGTTCGCTCTTCTGAAATGCCTACCATTTTTTCTCACAACTCAGTCACCATGAGTTTGCCAGAAGGTCGGTAG

Protein Analysis

835

Amino Acids

93.6

Weight (kDa)

6.69

Isoelectric Point (pI)

42.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 78 - 185 1.8e-32 D-mannose binding lectin
S_locus_glycop PF00954 218 - 323 3.5e-24 S-locus glycoprotein domain
PAN_2 PF08276 360 - 414 2.2e-13 PAN-like domain
Pkinase PF00069 517 - 717 8.1e-48 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 520 - 786 8.9e-50 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000564)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g44064 FvH4_6g44064 FvH4_6g44100 FvH4_6g44101 FvH4_6g51830
malus_domestica MD09G1094400.v1.1 MD09G1094800.v1.1 MD09G1095200.v1.1 MD09G1095900.v1.1 MD09G1096000.v1.1 MD09G1096200.v1.1 MD17G1083900.v1.1 MD17G1088500.v1.1
prunus_persica Prupe.3G224900_v2.0.a1 Prupe.3G224900_v2.0.a1 Prupe.3G224900_v2.0.a1 Prupe.3G224900_v2.0.a1 Prupe.3G224900_v2.0.a1 Prupe.3G224900_v2.0.a1 Prupe.3G225100_v2.0.a1 Prupe.3G225100_v2.0.a1 Prupe.3G225100_v2.0.a1 Prupe.3G225200_v2.0.a1 Prupe.3G225200_v2.0.a1
pyrus_communis pycom09g01920 pycom09g01930 pycom09g01940 pycom09g01950 pycom09g02000 pycom09g02050 pycom09g02070 pycom17g08540
rosa_chinensis RchiOBHm_Chr1g0318601 RchiOBHm_Chr1g0318761 RchiOBHm_Chr1g0342541 RchiOBHm_Chr2g0160651 RchiOBHm_Chr2g0160831 RchiOBHm_Chr4g0405661 RchiOBHm_Chr4g0405691 RchiOBHm_Chr4g0405701 RchiOBHm_Chr7g0238591
rosa_laevigata RLG00000002292 RLG00000008826 RLG00000008828 RLG00000008829 RLG00000021243 RLG00000021245 RLG00000030522
rosa_multiflora Rmu_co8432573.1_g000001 Rmu_sc0001200.1_g000041 Rmu_sc0001200.1_g000043 Rmu_sc0002545.1_g000017 Rmu_sc0002773.1_g000017 Rmu_sc0012550.1_g000008
rosa_roxburghii Rroxscaffold_2G00089500 Rroxscaffold_5G00350130 Rroxscaffold_5G00350180
rosa_rugosa Rorug01G0022300 Rorug04G0061600
rosa_samantha Rh1AG035300 Rh1CG034300 Rh1DG036100 Rh1DG149100 Rh2AG550900 Rh2BG564200 Rh2BG565500 Rh2CG535300 Rh2DG574400 Rh4AG133100 Rh4AG133200 Rh4AG133300 Rh4BG128200 Rh4CG141000 Rh4DG127700 Rh4DG127900
rosa_wichuraiana Rw2G045540 Rw2G045650 Rw4G010790 Rw4G010800 Rw4G010810 Rw4G010830 Rw7G027620 Rw7G027630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 128
Acc36I ACCTGC 1 cut(s) 128
AccBSI CCGCTC 1 cut(s) 425
AccI GTMKAC 2 cut(s) 502, 1590
AciI CCGC 2 cut(s) 425, 2139
AclI AACGTT 1 cut(s) 1324
AclWI GGATC 1 cut(s) 662
AcoI YGGCCR 1 cut(s) 115
AcsI RAATTY 5 cut(s) 352, 556, 1466, 1599, 1968
AcuI CTGAAG 2 cut(s) 1572, 2034
AfaI GTAC 4 cut(s) 206, 1772, 1878, 1895
AfiI CCNNNNNNNGG 6 cut(s) 240, 342, 486, 575, 1511, 1632
AflIII ACRYGT 1 cut(s) 2052
AjnI CCWGG 3 cut(s) 169, 336, 574
AjuI GAANNNNNNNTTGG 2 cut(s) 2326, 2358
AloI GAACNNNNNNTCC 2 cut(s) 661, 693
Alw21I GWGCWC 1 cut(s) 664
Alw26I GTCTC 5 cut(s) 811, 867, 1074, 1448, 2385
Alw44I GTGCAC 1 cut(s) 660
AlwI GGATC 1 cut(s) 662
AlwNI CAGNNNCTG 2 cut(s) 269, 1307
Ama87I CYCGRG 1 cut(s) 808
AoxI GGCC 3 cut(s) 115, 811, 1582
ApaLI GTGCAC 1 cut(s) 660
ApeKI GCWGC 7 cut(s) 80, 1043, 1298, 1301, 1472, 1532, 2153
ApoI RAATTY 5 cut(s) 352, 556, 1466, 1599, 1968
AspS9I GGNCC 3 cut(s) 811, 1583, 1921
AsuC2I CCSGG 1 cut(s) 602
AsuHPI GGTGA 9 cut(s) 107, 748, 1076, 1463, 1493, 2049, 2169, 2245, 2470
AsuII TTCGAA 1 cut(s) 1849
AsuNHI GCTAGC 1 cut(s) 595
AvaI CYCGRG 1 cut(s) 808
AvaII GGWCC 1 cut(s) 1921
BaeGI GKGCMC 1 cut(s) 664
BalI TGGCCA 1 cut(s) 117
BbsI GAAGAC 3 cut(s) 1418, 1846, 2334
Bbv12I GWGCWC 1 cut(s) 664
BbvI GCAGC 7 cut(s) 92, 1030, 1285, 1313, 1459, 1519, 2140
BccI CCATC 4 cut(s) 126, 296, 1061, 2187
BceAI ACGGC 1 cut(s) 1321
BciT130I CCWGG 3 cut(s) 171, 338, 576
BciVI GTATCC 2 cut(s) 1127, 2297
BclI TGATCA 1 cut(s) 469
BcnI CCSGG 1 cut(s) 602
BcoDI GTCTC 5 cut(s) 811, 867, 1074, 1448, 2385
BfaI CTAG 7 cut(s) 77, 432, 596, 912, 1047, 1091, 1899
BfmI CTRYAG 2 cut(s) 135, 1299
BfuAI ACCTGC 1 cut(s) 128
BfuI GTATCC 2 cut(s) 1127, 2297
BglI GCCNNNNNGGC 2 cut(s) 457, 970
BisI GCNGC 7 cut(s) 81, 1044, 1299, 1302, 1473, 1533, 2154
BlsI GCNGC 7 cut(s) 82, 1045, 1300, 1303, 1474, 1534, 2155
BmcAI AGTACT 2 cut(s) 1878, 1895
Bme1390I CCNGG 4 cut(s) 171, 338, 576, 602
Bme18I GGWCC 1 cut(s) 1921
BmeT110I CYCGRG 1 cut(s) 808
BmgT120I GGNCC 3 cut(s) 811, 1583, 1921
BmiI GGNNCC 2 cut(s) 699, 1922
BmrFI CCNGG 4 cut(s) 171, 338, 576, 602
BmsI GCATC 5 cut(s) 303, 782, 1483, 1798, 1820
BmtI GCTAGC 1 cut(s) 599
BpiI GAAGAC 3 cut(s) 1418, 1846, 2334
BpmI CTGGAG 2 cut(s) 1497, 2304
Bpu14I TTCGAA 1 cut(s) 1849
BpuEI CTTGAG 5 cut(s) 44, 310, 439, 611, 1854
BpuMI CCSGG 1 cut(s) 602
Bsa29I ATCGAT 1 cut(s) 2238
BsaBI GATNNNNATC 1 cut(s) 1760
BsaI GGTCTC 1 cut(s) 867
BsaJI CCNNGG 4 cut(s) 336, 600, 665, 930
BsaXI ACNNNNNCTCC 2 cut(s) 830, 860
Bsc4I CCNNNNNNNGG 6 cut(s) 240, 342, 486, 575, 1511, 1632
Bse118I RCCGGY 1 cut(s) 969
Bse1I ACTGG 3 cut(s) 263, 325, 1023
Bse3DI GCAATG 4 cut(s) 1109, 1183, 1863, 2374
Bse8I GATNNNNATC 1 cut(s) 1760
BseBI CCWGG 3 cut(s) 171, 338, 576
BseCI ATCGAT 1 cut(s) 2238
BseDI CCNNGG 4 cut(s) 336, 600, 665, 930
BseGI GGATG 4 cut(s) 472, 926, 1911, 2198
BseJI GATNNNNATC 1 cut(s) 1760
BseLI CCNNNNNNNGG 6 cut(s) 240, 342, 486, 575, 1511, 1632
BseMI GCAATG 4 cut(s) 1109, 1183, 1863, 2374
BseMII CTCAG 4 cut(s) 33, 216, 2079, 2487
BseNI ACTGG 3 cut(s) 263, 325, 1023
BseRI GAGGAG 1 cut(s) 1760
BseSI GKGCMC 1 cut(s) 664
BseXI GCAGC 7 cut(s) 92, 1030, 1285, 1313, 1459, 1519, 2140
BsgI GTGCAG 1 cut(s) 555
BshFI GGCC 3 cut(s) 117, 813, 1584
BshVI ATCGAT 1 cut(s) 2238
BsiHKAI GWGCWC 1 cut(s) 664
BsiHKCI CYCGRG 1 cut(s) 808
BsiSI CCGG 2 cut(s) 602, 970
BslFI GGGAC 4 cut(s) 998, 1072, 1324, 1934
BslI CCNNNNNNNGG 6 cut(s) 240, 342, 486, 575, 1511, 1632
BsmAI GTCTC 5 cut(s) 811, 867, 1074, 1448, 2385
BsmBI CGTCTC 2 cut(s) 1074, 2385
BsmFI GGGAC 4 cut(s) 998, 1072, 1324, 1934
BsmI GAATGC 2 cut(s) 1045, 1184
BsnI GGCC 3 cut(s) 117, 813, 1584
Bso31I GGTCTC 1 cut(s) 867
BsoBI CYCGRG 1 cut(s) 808
Bsp119I TTCGAA 1 cut(s) 1849
Bsp1286I GDGCHC 1 cut(s) 664
Bsp143I GATC 8 cut(s) 469, 654, 772, 1160, 1506, 1761, 2235, 2326
Bsp19I CCATGG 1 cut(s) 665
BspACI CCGC 2 cut(s) 425, 2139
BspANI GGCC 3 cut(s) 117, 813, 1584
BspCNI CTCAG 4 cut(s) 32, 217, 2080, 2486
BspDI ATCGAT 1 cut(s) 2238
BspHI TCATGA 1 cut(s) 1671
BspLI GGNNCC 2 cut(s) 699, 1922
BspMAI CTGCAG 2 cut(s) 139, 1303
BspMI ACCTGC 1 cut(s) 128
BspOI GCTAGC 1 cut(s) 599
BspPI GGATC 1 cut(s) 662
BspQI GCTCTTC 1 cut(s) 2446
BspT104I TTCGAA 1 cut(s) 1849
BspTNI GGTCTC 1 cut(s) 867
BsrBI CCGCTC 1 cut(s) 425
BsrDI GCAATG 4 cut(s) 1109, 1183, 1863, 2374
BsrFI RCCGGY 1 cut(s) 969
BsrI ACTGG 3 cut(s) 263, 325, 1023
BssAI RCCGGY 1 cut(s) 969
BssECI CCNNGG 4 cut(s) 336, 600, 665, 930
BssMI GATC 8 cut(s) 469, 654, 772, 1160, 1506, 1761, 2235, 2326
BssT1I CCWWGG 2 cut(s) 665, 930
Bst2UI CCWGG 3 cut(s) 171, 338, 576
Bst6I CTCTTC 4 cut(s) 1737, 2215, 2388, 2446
BstBI TTCGAA 1 cut(s) 1849
BstC8I GCNNGC 1 cut(s) 597
BstDEI CTNAG 5 cut(s) 19, 225, 298, 2088, 2473
BstDSI CCRYGG 1 cut(s) 665
BstENI CCTNNNNNAGG 1 cut(s) 573
BstF5I GGATG 4 cut(s) 472, 926, 1911, 2198
BstKTI GATC 8 cut(s) 472, 657, 775, 1163, 1509, 1764, 2238, 2329
BstMAI GTCTC 5 cut(s) 811, 867, 1074, 1448, 2385
BstMBI GATC 8 cut(s) 469, 654, 772, 1160, 1506, 1761, 2235, 2326
BstMWI GCNNNNNNNGC 5 cut(s) 457, 970, 1049, 1529, 2195
BstNI CCWGG 3 cut(s) 171, 338, 576
BstNSI RCATGY 2 cut(s) 1777, 2056
BstSCI CCNGG 4 cut(s) 169, 336, 574, 600
BstSFI CTRYAG 2 cut(s) 135, 1299
BstSLI GKGCMC 1 cut(s) 664
BstV1I GCAGC 7 cut(s) 92, 1030, 1285, 1313, 1459, 1519, 2140
BstV2I GAAGAC 3 cut(s) 1418, 1846, 2334
BstXI CCANNNNNNTGG 2 cut(s) 337, 931
Bsu15I ATCGAT 1 cut(s) 2238
BsuI GTATCC 2 cut(s) 1127, 2297
BsuRI GGCC 3 cut(s) 117, 813, 1584
BsuTUI ATCGAT 1 cut(s) 2238
BtgI CCRYGG 1 cut(s) 665
BtsCI GGATG 4 cut(s) 472, 926, 1911, 2198
BtsI GCAGTG 3 cut(s) 851, 1037, 1188
BtsIMutI CAGTG 6 cut(s) 664, 851, 884, 1037, 1188, 1422
BveI ACCTGC 1 cut(s) 128
Cac8I GCNNGC 1 cut(s) 597
CaiI CAGNNNCTG 2 cut(s) 269, 1307
CciI TCATGA 1 cut(s) 1671
Cfr10I RCCGGY 1 cut(s) 969
Cfr13I GGNCC 3 cut(s) 811, 1583, 1921
ClaI ATCGAT 1 cut(s) 2238
CsiI ACCWGGT 2 cut(s) 169, 574
Csp6I GTAC 4 cut(s) 205, 1771, 1877, 1894
CviQI GTAC 4 cut(s) 205, 1771, 1877, 1894
DdeI CTNAG 5 cut(s) 19, 225, 298, 2088, 2473
DpnI GATC 8 cut(s) 471, 656, 774, 1162, 1508, 1763, 2237, 2328
DpnII GATC 8 cut(s) 469, 654, 772, 1160, 1506, 1761, 2235, 2326
DraI TTTAAA 2 cut(s) 357, 1173
EaeI YGGCCR 1 cut(s) 115
Eam1104I CTCTTC 4 cut(s) 1737, 2215, 2388, 2446
EarI CTCTTC 4 cut(s) 1737, 2215, 2388, 2446
Eco130I CCWWGG 2 cut(s) 665, 930
Eco31I GGTCTC 1 cut(s) 867
Eco47I GGWCC 1 cut(s) 1921
Eco57I CTGAAG 2 cut(s) 1572, 2034
Eco88I CYCGRG 1 cut(s) 808
EcoNI CCTNNNNNAGG 1 cut(s) 573
EcoO109I RGGNCCY 1 cut(s) 1921
EcoRI GAATTC 1 cut(s) 556
EcoRII CCWGG 3 cut(s) 169, 336, 574
EcoT14I CCWWGG 2 cut(s) 665, 930
EcoT22I ATGCAT 1 cut(s) 1813
ErhI CCWWGG 2 cut(s) 665, 930
Esp3I CGTCTC 2 cut(s) 1074, 2385
FalI AAGNNNNNCTT 4 cut(s) 378, 410, 1863, 1895
FaqI GGGAC 4 cut(s) 998, 1072, 1324, 1934
FauI CCCGC 1 cut(s) 418
FauNDI CATATG 2 cut(s) 200, 2355
FbaI TGATCA 1 cut(s) 469
FblI GTMKAC 2 cut(s) 502, 1590
Fnu4HI GCNGC 7 cut(s) 81, 1044, 1299, 1302, 1473, 1533, 2154
FokI GGATG 4 cut(s) 459, 913, 1898, 2205
Fsp4HI GCNGC 7 cut(s) 81, 1044, 1299, 1302, 1473, 1533, 2154
FspBI CTAG 7 cut(s) 77, 432, 596, 912, 1047, 1091, 1899
GluI GCNGC 7 cut(s) 81, 1044, 1299, 1302, 1473, 1533, 2154
GsuI CTGGAG 2 cut(s) 1497, 2304
HaeIII GGCC 3 cut(s) 117, 813, 1584
HapII CCGG 2 cut(s) 602, 970
HincII GTYRAC 1 cut(s) 348
HindII GTYRAC 1 cut(s) 348
HindIII AAGCTT 2 cut(s) 463, 2274
HinfI GANTC 5 cut(s) 510, 817, 1960, 2243, 2372
HpaII CCGG 2 cut(s) 602, 970
HphI GGTGA 9 cut(s) 107, 748, 1076, 1463, 1493, 2049, 2169, 2245, 2470
Hpy166II GTNNAC 7 cut(s) 73, 348, 503, 662, 680, 849, 1591
Hpy8I GTNNAC 7 cut(s) 73, 348, 503, 662, 680, 849, 1591
HpyAV CCTTC 5 cut(s) 946, 1853, 2210, 2400, 2489
HpyCH4IV ACGT 4 cut(s) 1324, 1843, 2258, 2378
HpyF10VI GCNNNNNNNGC 5 cut(s) 457, 970, 1049, 1529, 2195
HpyF3I CTNAG 5 cut(s) 19, 225, 298, 2088, 2473
HpySE526I ACGT 4 cut(s) 1324, 1843, 2258, 2378
Ksp22I TGATCA 1 cut(s) 469
Kzo9I GATC 8 cut(s) 469, 654, 772, 1160, 1506, 1761, 2235, 2326
LguI GCTCTTC 1 cut(s) 2446
LmnI GCTCC 5 cut(s) 703, 800, 1704, 1785, 2323
Lsp1109I GCAGC 7 cut(s) 92, 1030, 1285, 1313, 1459, 1519, 2140
LweI GCATC 5 cut(s) 303, 782, 1483, 1798, 1820
MabI ACCWGGT 2 cut(s) 169, 574
MaeI CTAG 7 cut(s) 77, 432, 596, 912, 1047, 1091, 1899
MaeII ACGT 4 cut(s) 1324, 1843, 2258, 2378
MaeIII GTNAC 9 cut(s) 434, 730, 1265, 1357, 1366, 1934, 2048, 2055, 2476
MalI GATC 8 cut(s) 471, 656, 774, 1162, 1508, 1763, 2237, 2328
MbiI CCGCTC 1 cut(s) 425
MboI GATC 8 cut(s) 469, 654, 772, 1160, 1506, 1761, 2235, 2326
MfeI CAATTG 1 cut(s) 1353
MhlI GDGCHC 1 cut(s) 664
MlsI TGGCCA 1 cut(s) 117
MluNI TGGCCA 1 cut(s) 117
MmeI TCCRAC 5 cut(s) 500, 816, 1726, 1926, 2106
MnlI CCTC 9 cut(s) 491, 1472, 1564, 1568, 1738, 1741, 2216, 2224, 2276
Mox20I TGGCCA 1 cut(s) 117
Mph1103I ATGCAT 1 cut(s) 1813
MscI TGGCCA 1 cut(s) 117
MseI TTAA 7 cut(s) 356, 1172, 1314, 1328, 1332, 1517, 1682
MslI CAYNNNNRTG 2 cut(s) 1236, 2268
Msp20I TGGCCA 1 cut(s) 117
MspA1I CMGCKG 2 cut(s) 83, 2324
MspI CCGG 2 cut(s) 602, 970
MspR9I CCNGG 4 cut(s) 171, 338, 576, 602
MunI CAATTG 1 cut(s) 1353
Mva1269I GAATGC 2 cut(s) 1045, 1184
MvaI CCWGG 3 cut(s) 171, 338, 576
MwoI GCNNNNNNNGC 5 cut(s) 457, 970, 1049, 1529, 2195
NciI CCSGG 1 cut(s) 602
NcoI CCATGG 1 cut(s) 665
NdeI CATATG 2 cut(s) 200, 2355
NdeII GATC 8 cut(s) 469, 654, 772, 1160, 1506, 1761, 2235, 2326
NheI GCTAGC 1 cut(s) 595
NlaIV GGNNCC 2 cut(s) 699, 1922
NmuCI GTSAC 5 cut(s) 1265, 1357, 1366, 2055, 2476
NsiI ATGCAT 1 cut(s) 1813
NspI RCATGY 2 cut(s) 1777, 2056
NspV TTCGAA 1 cut(s) 1849
PacI TTAATTAA 1 cut(s) 1332
PagI TCATGA 1 cut(s) 1671
PaqCI CACCTGC 1 cut(s) 128
PciI ACATGT 1 cut(s) 2052
PciSI GCTCTTC 1 cut(s) 2446
PctI GAATGC 2 cut(s) 1045, 1184
PfeI GAWTC 5 cut(s) 510, 817, 1960, 2243, 2372
PkrI GCNGC 7 cut(s) 82, 1045, 1300, 1303, 1474, 1534, 2155
PpuMI RGGWCCY 1 cut(s) 1921
PscI ACATGT 1 cut(s) 2052
Psp1406I AACGTT 1 cut(s) 1324
Psp5II RGGWCCY 1 cut(s) 1921
Psp6I CCWGG 3 cut(s) 169, 336, 574
PspGI CCWGG 3 cut(s) 169, 336, 574
PspN4I GGNNCC 2 cut(s) 699, 1922
PspPI GGNCC 3 cut(s) 811, 1583, 1921
PspPPI RGGWCCY 1 cut(s) 1921
PstI CTGCAG 2 cut(s) 139, 1303
PstNI CAGNNNCTG 2 cut(s) 269, 1307
PvuII CAGCTG 2 cut(s) 83, 2324
RsaI GTAC 4 cut(s) 206, 1772, 1878, 1895
RsaNI GTAC 4 cut(s) 205, 1771, 1877, 1894
RseI CAYNNNNRTG 2 cut(s) 1236, 2268
SapI GCTCTTC 1 cut(s) 2446
SaqAI TTAA 7 cut(s) 356, 1172, 1314, 1328, 1332, 1517, 1682
SatI GCNGC 7 cut(s) 81, 1044, 1299, 1302, 1473, 1533, 2154
Sau3AI GATC 8 cut(s) 469, 654, 772, 1160, 1506, 1761, 2235, 2326
Sau96I GGNCC 3 cut(s) 811, 1583, 1921
ScaI AGTACT 2 cut(s) 1878, 1895
ScrFI CCNGG 4 cut(s) 171, 338, 576, 602
SduI GDGCHC 1 cut(s) 664
SexAI ACCWGGT 2 cut(s) 169, 574
SfaNI GCATC 5 cut(s) 303, 782, 1483, 1798, 1820
SfcI CTRYAG 2 cut(s) 135, 1299
SfuI TTCGAA 1 cut(s) 1849
SinI GGWCC 1 cut(s) 1921
SmiMI CAYNNNNRTG 2 cut(s) 1236, 2268
SmlI CTYRAG 5 cut(s) 59, 289, 418, 590, 1869
SmoI CTYRAG 5 cut(s) 59, 289, 418, 590, 1869
SsiI CCGC 2 cut(s) 425, 2139
SspMI CTAG 7 cut(s) 77, 432, 596, 912, 1047, 1091, 1899
StyD4I CCNGG 4 cut(s) 169, 336, 574, 600
StyI CCWWGG 2 cut(s) 665, 930
TaiI ACGT 4 cut(s) 1327, 1846, 2261, 2381
TaqI TCGA 6 cut(s) 790, 1149, 1849, 2238, 2268, 2329
TaqII GACCGA 1 cut(s) 274
TatI WGTACW 3 cut(s) 1770, 1876, 1893
TfiI GAWTC 5 cut(s) 510, 817, 1960, 2243, 2372
Tru1I TTAA 7 cut(s) 356, 1172, 1314, 1328, 1332, 1517, 1682
Tru9I TTAA 7 cut(s) 356, 1172, 1314, 1328, 1332, 1517, 1682
TscAI CASTG 6 cut(s) 664, 851, 884, 1037, 1195, 1422
TseFI GTSAC 5 cut(s) 1265, 1357, 1366, 2055, 2476
TseI GCWGC 7 cut(s) 80, 1043, 1298, 1301, 1472, 1532, 2153
Tsp45I GTSAC 5 cut(s) 1265, 1357, 1366, 2055, 2476
TspRI CASTG 6 cut(s) 664, 851, 884, 1037, 1195, 1422
VneI GTGCAC 1 cut(s) 660
VpaK11BI GGWCC 1 cut(s) 1921
XagI CCTNNNNNAGG 1 cut(s) 573
XapI RAATTY 5 cut(s) 352, 556, 1466, 1599, 1968
XbaI TCTAGA 2 cut(s) 1090, 1898
XceI RCATGY 2 cut(s) 1777, 2056
XcmI CCANNNNNNNNNTGG 3 cut(s) 110, 483, 1786
XmiI GTMKAC 2 cut(s) 502, 1590
XspI CTAG 7 cut(s) 77, 432, 596, 912, 1047, 1091, 1899
ZrmI AGTACT 2 cut(s) 1878, 1895
Zsp2I ATGCAT 1 cut(s) 1813
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.