serine threonine-protein kinase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 846 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 717 |
| AciI | CCGC | 3 cut(s) | 261, 281, 516 |
| AclWI | GGATC | 2 cut(s) | 416, 429 |
| AcsI | RAATTY | 7 cut(s) | 208, 393, 412, 552, 706, 721, 1119 |
| AfaI | GTAC | 3 cut(s) | 62, 240, 1271 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 198, 342, 431, 717 |
| AflIII | ACRYGT | 2 cut(s) | 1162, 1318 |
| AjnI | CCWGG | 3 cut(s) | 25, 349, 430 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1238, 1270 |
| AluBI | AGCT | 5 cut(s) | 10, 34, 321, 1136, 1142 |
| AluI | AGCT | 5 cut(s) | 10, 34, 321, 1136, 1142 |
| Alw21I | GWGCWC | 2 cut(s) | 979, 1028 |
| Alw26I | GTCTC | 1 cut(s) | 738 |
| Alw44I | GTGCAC | 1 cut(s) | 1024 |
| AlwI | GGATC | 2 cut(s) | 416, 429 |
| Ama87I | CYCGRG | 1 cut(s) | 673 |
| ApaLI | GTGCAC | 1 cut(s) | 1024 |
| ApeKI | GCWGC | 4 cut(s) | 1044, 1047, 1136, 1139 |
| ApoI | RAATTY | 7 cut(s) | 208, 393, 412, 552, 706, 721, 1119 |
| AspLEI | GCGC | 1 cut(s) | 430 |
| AspS9I | GGNCC | 2 cut(s) | 510, 518 |
| AsuC2I | CCSGG | 1 cut(s) | 194 |
| AvaI | CYCGRG | 1 cut(s) | 673 |
| AvaII | GGWCC | 2 cut(s) | 510, 518 |
| BaeGI | GKGCMC | 1 cut(s) | 1028 |
| BamHI | GGATCC | 1 cut(s) | 421 |
| BanII | GRGCYC | 1 cut(s) | 287 |
| Bbv12I | GWGCWC | 2 cut(s) | 979, 1028 |
| BbvI | GCAGC | 4 cut(s) | 1056, 1059, 1123, 1151 |
| BccI | CCATC | 3 cut(s) | 152, 1046, 1103 |
| BceAI | ACGGC | 2 cut(s) | 1159, 1288 |
| BciT130I | CCWGG | 3 cut(s) | 27, 351, 432 |
| BclI | TGATCA | 1 cut(s) | 325 |
| BcnI | CCSGG | 1 cut(s) | 194 |
| BcoDI | GTCTC | 1 cut(s) | 738 |
| BfaI | CTAG | 3 cut(s) | 11, 288, 498 |
| BfmI | CTRYAG | 2 cut(s) | 839, 1137 |
| BfuAI | ACCTGC | 1 cut(s) | 846 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 313 |
| BisI | GCNGC | 4 cut(s) | 1045, 1048, 1137, 1140 |
| BlsI | GCNGC | 4 cut(s) | 1046, 1049, 1138, 1141 |
| Bme1390I | CCNGG | 4 cut(s) | 27, 194, 351, 432 |
| Bme18I | GGWCC | 2 cut(s) | 510, 518 |
| BmeT110I | CYCGRG | 1 cut(s) | 673 |
| BmgT120I | GGNCC | 2 cut(s) | 510, 518 |
| BmiI | GGNNCC | 1 cut(s) | 423 |
| BmrFI | CCNGG | 4 cut(s) | 27, 194, 351, 432 |
| BmsI | GCATC | 1 cut(s) | 159 |
| BpmI | CTGGAG | 1 cut(s) | 763 |
| BpuEI | CTTGAG | 2 cut(s) | 166, 467 |
| BpuMI | CCSGG | 1 cut(s) | 194 |
| BsaI | GGTCTC | 1 cut(s) | 738 |
| BsaJI | CCNNGG | 3 cut(s) | 192, 521, 672 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 613, 643 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 198, 342, 431, 717 |
| Bse1I | ACTGG | 4 cut(s) | 119, 780, 891, 1021 |
| BseBI | CCWGG | 3 cut(s) | 27, 351, 432 |
| BseDI | CCNNGG | 3 cut(s) | 192, 521, 672 |
| BseGI | GGATG | 6 cut(s) | 328, 385, 532, 794, 881, 1061 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 198, 342, 431, 717 |
| BseMII | CTCAG | 1 cut(s) | 72 |
| BseNI | ACTGG | 4 cut(s) | 119, 780, 891, 1021 |
| BseSI | GKGCMC | 1 cut(s) | 1028 |
| BseXI | GCAGC | 4 cut(s) | 1056, 1059, 1123, 1151 |
| BsiHKAI | GWGCWC | 2 cut(s) | 979, 1028 |
| BsiHKCI | CYCGRG | 1 cut(s) | 673 |
| BsiSI | CCGG | 1 cut(s) | 194 |
| BslFI | GGGAC | 3 cut(s) | 784, 866, 1162 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 198, 342, 431, 717 |
| BsmAI | GTCTC | 1 cut(s) | 738 |
| BsmFI | GGGAC | 3 cut(s) | 784, 866, 1162 |
| BsmI | GAATGC | 2 cut(s) | 913, 1022 |
| Bso31I | GGTCTC | 1 cut(s) | 738 |
| BsoBI | CYCGRG | 1 cut(s) | 673 |
| Bsp1286I | GDGCHC | 3 cut(s) | 287, 979, 1028 |
| Bsp1407I | TGTACA | 1 cut(s) | 238 |
| Bsp143I | GATC | 3 cut(s) | 325, 421, 998 |
| Bsp19I | CCATGG | 1 cut(s) | 521 |
| BspACI | CCGC | 3 cut(s) | 261, 281, 516 |
| BspCNI | CTCAG | 1 cut(s) | 73 |
| BspLI | GGNNCC | 1 cut(s) | 423 |
| BspMAI | CTGCAG | 2 cut(s) | 843, 1141 |
| BspMI | ACCTGC | 1 cut(s) | 846 |
| BspPI | GGATC | 2 cut(s) | 416, 429 |
| BspTNI | GGTCTC | 1 cut(s) | 738 |
| BsrGI | TGTACA | 1 cut(s) | 238 |
| BsrI | ACTGG | 4 cut(s) | 119, 780, 891, 1021 |
| BssECI | CCNNGG | 3 cut(s) | 192, 521, 672 |
| BssMI | GATC | 3 cut(s) | 325, 421, 998 |
| BssT1I | CCWWGG | 1 cut(s) | 521 |
| Bst2UI | CCWGG | 3 cut(s) | 27, 351, 432 |
| Bst4CI | ACNGT | 3 cut(s) | 91, 747, 1330 |
| BstAUI | TGTACA | 1 cut(s) | 238 |
| BstC8I | GCNNGC | 2 cut(s) | 283, 1006 |
| BstDEI | CTNAG | 3 cut(s) | 81, 154, 980 |
| BstDSI | CCRYGG | 1 cut(s) | 521 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 429 |
| BstF5I | GGATG | 6 cut(s) | 328, 385, 532, 794, 881, 1061 |
| BstHHI | GCGC | 1 cut(s) | 430 |
| BstKTI | GATC | 3 cut(s) | 328, 424, 1001 |
| BstMAI | GTCTC | 1 cut(s) | 738 |
| BstMBI | GATC | 3 cut(s) | 325, 421, 998 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 313, 838 |
| BstNI | CCWGG | 3 cut(s) | 27, 351, 432 |
| BstSCI | CCNGG | 4 cut(s) | 25, 192, 349, 430 |
| BstSFI | CTRYAG | 2 cut(s) | 839, 1137 |
| BstSLI | GKGCMC | 1 cut(s) | 1028 |
| BstV1I | GCAGC | 4 cut(s) | 1056, 1059, 1123, 1151 |
| BstX2I | RGATCY | 1 cut(s) | 421 |
| BstYI | RGATCY | 1 cut(s) | 421 |
| BtgI | CCRYGG | 1 cut(s) | 521 |
| BtgZI | GCGATG | 1 cut(s) | 1065 |
| BtsCI | GGATG | 6 cut(s) | 328, 385, 532, 794, 881, 1061 |
| BtsI | GCAGTG | 2 cut(s) | 722, 1026 |
| BtsIMutI | CAGTG | 5 cut(s) | 722, 752, 898, 1026, 1028 |
| BveI | ACCTGC | 1 cut(s) | 846 |
| Cac8I | GCNNGC | 2 cut(s) | 283, 1006 |
| CfoI | GCGC | 1 cut(s) | 430 |
| Cfr13I | GGNCC | 2 cut(s) | 510, 518 |
| CsiI | ACCWGGT | 1 cut(s) | 25 |
| Csp6I | GTAC | 3 cut(s) | 61, 239, 1270 |
| CviAII | CATG | 4 cut(s) | 405, 522, 696, 1070 |
| CviQI | GTAC | 3 cut(s) | 61, 239, 1270 |
| DdeI | CTNAG | 3 cut(s) | 81, 154, 980 |
| DpnI | GATC | 3 cut(s) | 327, 423, 1000 |
| DpnII | GATC | 3 cut(s) | 325, 421, 998 |
| DraI | TTTAAA | 1 cut(s) | 213 |
| Eco130I | CCWWGG | 1 cut(s) | 521 |
| Eco24I | GRGCYC | 1 cut(s) | 287 |
| Eco31I | GGTCTC | 1 cut(s) | 738 |
| Eco47I | GGWCC | 2 cut(s) | 510, 518 |
| Eco88I | CYCGRG | 1 cut(s) | 673 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 429 |
| EcoRI | GAATTC | 1 cut(s) | 706 |
| EcoRII | CCWGG | 3 cut(s) | 25, 349, 430 |
| EcoT14I | CCWWGG | 1 cut(s) | 521 |
| EcoT38I | GRGCYC | 1 cut(s) | 287 |
| ErhI | CCWWGG | 1 cut(s) | 521 |
| FaeI | CATG | 4 cut(s) | 408, 525, 699, 1073 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 234, 266, 963, 995 |
| FaqI | GGGAC | 3 cut(s) | 784, 866, 1162 |
| FatI | CATG | 4 cut(s) | 404, 521, 695, 1069 |
| FauI | CCCGC | 1 cut(s) | 274 |
| FauNDI | CATATG | 1 cut(s) | 56 |
| FbaI | TGATCA | 1 cut(s) | 325 |
| Fnu4HI | GCNGC | 4 cut(s) | 1045, 1048, 1137, 1140 |
| FokI | GGATG | 6 cut(s) | 315, 372, 539, 781, 888, 1068 |
| FriOI | GRGCYC | 1 cut(s) | 287 |
| Fsp4HI | GCNGC | 4 cut(s) | 1045, 1048, 1137, 1140 |
| FspBI | CTAG | 3 cut(s) | 11, 288, 498 |
| GlaI | GCGC | 1 cut(s) | 429 |
| GluI | GCNGC | 4 cut(s) | 1045, 1048, 1137, 1140 |
| GsuI | CTGGAG | 1 cut(s) | 763 |
| HapII | CCGG | 1 cut(s) | 194 |
| HhaI | GCGC | 1 cut(s) | 430 |
| Hin1II | CATG | 4 cut(s) | 408, 525, 699, 1073 |
| Hin6I | GCGC | 1 cut(s) | 428 |
| HinP1I | GCGC | 1 cut(s) | 428 |
| HincII | GTYRAC | 1 cut(s) | 204 |
| HindII | GTYRAC | 1 cut(s) | 204 |
| HindIII | AAGCTT | 1 cut(s) | 319 |
| HinfI | GANTC | 5 cut(s) | 182, 366, 682, 854, 1084 |
| HpaII | CCGG | 1 cut(s) | 194 |
| Hpy166II | GTNNAC | 2 cut(s) | 204, 1026 |
| Hpy188I | TCNGA | 4 cut(s) | 82, 304, 1133, 1147 |
| Hpy188III | TCNNGA | 9 cut(s) | 145, 175, 274, 446, 498, 557, 675, 882, 1081 |
| Hpy8I | GTNNAC | 2 cut(s) | 204, 1026 |
| HpyAV | CCTTC | 2 cut(s) | 814, 965 |
| HpyCH4III | ACNGT | 3 cut(s) | 91, 747, 1330 |
| HpyCH4IV | ACGT | 2 cut(s) | 1162, 1318 |
| HpyCH4V | TGCA | 7 cut(s) | 786, 810, 841, 1026, 1090, 1139, 1190 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 313, 838 |
| HpyF3I | CTNAG | 3 cut(s) | 81, 154, 980 |
| HpySE526I | ACGT | 2 cut(s) | 1162, 1318 |
| Hsp92II | CATG | 4 cut(s) | 408, 525, 699, 1073 |
| HspAI | GCGC | 1 cut(s) | 428 |
| Ksp22I | TGATCA | 1 cut(s) | 325 |
| Kzo9I | GATC | 3 cut(s) | 325, 421, 998 |
| Lsp1109I | GCAGC | 4 cut(s) | 1056, 1059, 1123, 1151 |
| LweI | GCATC | 1 cut(s) | 159 |
| MabI | ACCWGGT | 1 cut(s) | 25 |
| MaeI | CTAG | 3 cut(s) | 11, 288, 498 |
| MaeII | ACGT | 2 cut(s) | 1162, 1318 |
| MaeIII | GTNAC | 2 cut(s) | 595, 1198 |
| MalI | GATC | 3 cut(s) | 327, 423, 1000 |
| MboI | GATC | 3 cut(s) | 325, 421, 998 |
| MboII | GAAGA | 5 cut(s) | 10, 129, 421, 897, 1258 |
| MflI | RGATCY | 1 cut(s) | 421 |
| MhlI | GDGCHC | 3 cut(s) | 287, 979, 1028 |
| MlyI | GAGTC | 2 cut(s) | 191, 848 |
| MmeI | TCCRAC | 3 cut(s) | 356, 681, 1170 |
| MnlI | CCTC | 7 cut(s) | 347, 412, 454, 558, 682, 1109, 1322 |
| MseI | TTAA | 5 cut(s) | 212, 947, 1010, 1152, 1170 |
| MslI | CAYNNNNRTG | 1 cut(s) | 1074 |
| MspA1I | CMGCKG | 1 cut(s) | 516 |
| MspI | CCGG | 1 cut(s) | 194 |
| MspR9I | CCNGG | 4 cut(s) | 27, 194, 351, 432 |
| Mva1269I | GAATGC | 2 cut(s) | 913, 1022 |
| MvaI | CCWGG | 3 cut(s) | 27, 351, 432 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 313, 838 |
| NciI | CCSGG | 1 cut(s) | 194 |
| NcoI | CCATGG | 1 cut(s) | 521 |
| NdeI | CATATG | 1 cut(s) | 56 |
| NdeII | GATC | 3 cut(s) | 325, 421, 998 |
| NlaIII | CATG | 4 cut(s) | 408, 525, 699, 1073 |
| NlaIV | GGNNCC | 1 cut(s) | 423 |
| PctI | GAATGC | 2 cut(s) | 913, 1022 |
| PfeI | GAWTC | 3 cut(s) | 366, 682, 1084 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 717 |
| PkrI | GCNGC | 4 cut(s) | 1046, 1049, 1138, 1141 |
| PleI | GAGTC | 2 cut(s) | 190, 848 |
| PpsI | GAGTC | 2 cut(s) | 190, 848 |
| Psp6I | CCWGG | 3 cut(s) | 25, 349, 430 |
| PspGI | CCWGG | 3 cut(s) | 25, 349, 430 |
| PspN4I | GGNNCC | 1 cut(s) | 423 |
| PspPI | GGNCC | 2 cut(s) | 510, 518 |
| PstI | CTGCAG | 2 cut(s) | 843, 1141 |
| PsuI | RGATCY | 1 cut(s) | 421 |
| RsaI | GTAC | 3 cut(s) | 62, 240, 1271 |
| RsaNI | GTAC | 3 cut(s) | 61, 239, 1270 |
| RseI | CAYNNNNRTG | 1 cut(s) | 1074 |
| SaqAI | TTAA | 5 cut(s) | 212, 947, 1010, 1152, 1170 |
| SatI | GCNGC | 4 cut(s) | 1045, 1048, 1137, 1140 |
| Sau3AI | GATC | 3 cut(s) | 325, 421, 998 |
| Sau96I | GGNCC | 2 cut(s) | 510, 518 |
| SchI | GAGTC | 2 cut(s) | 191, 848 |
| ScrFI | CCNGG | 4 cut(s) | 27, 194, 351, 432 |
| SduI | GDGCHC | 3 cut(s) | 287, 979, 1028 |
| SexAI | ACCWGGT | 1 cut(s) | 25 |
| SfaNI | GCATC | 1 cut(s) | 159 |
| SfcI | CTRYAG | 2 cut(s) | 839, 1137 |
| SinI | GGWCC | 2 cut(s) | 510, 518 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 1074 |
| SmlI | CTYRAG | 2 cut(s) | 145, 446 |
| SmoI | CTYRAG | 2 cut(s) | 145, 446 |
| SsiI | CCGC | 3 cut(s) | 261, 281, 516 |
| SspMI | CTAG | 3 cut(s) | 11, 288, 498 |
| StyD4I | CCNGG | 4 cut(s) | 25, 192, 349, 430 |
| StyI | CCWWGG | 1 cut(s) | 521 |
| TaaI | ACNGT | 3 cut(s) | 91, 747, 1330 |
| TaiI | ACGT | 2 cut(s) | 1165, 1321 |
| TaqI | TCGA | 2 cut(s) | 987, 1283 |
| TaqII | GACCGA | 1 cut(s) | 130 |
| TatI | WGTACW | 1 cut(s) | 238 |
| TfiI | GAWTC | 3 cut(s) | 366, 682, 1084 |
| Tru1I | TTAA | 5 cut(s) | 212, 947, 1010, 1152, 1170 |
| Tru9I | TTAA | 5 cut(s) | 212, 947, 1010, 1152, 1170 |
| TscAI | CASTG | 5 cut(s) | 722, 752, 898, 1028, 1033 |
| TseI | GCWGC | 4 cut(s) | 1044, 1047, 1136, 1139 |
| TspDTI | ATGAA | 7 cut(s) | 393, 462, 543, 624, 984, 1029, 1278 |
| TspRI | CASTG | 5 cut(s) | 722, 752, 898, 1028, 1033 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 717 |
| VneI | GTGCAC | 1 cut(s) | 1024 |
| VpaK11BI | GGWCC | 2 cut(s) | 510, 518 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 429 |
| XapI | RAATTY | 7 cut(s) | 208, 393, 412, 552, 706, 721, 1119 |
| XbaI | TCTAGA | 1 cut(s) | 497 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 339 |
| XspI | CTAG | 3 cut(s) | 11, 288, 498 |