MD09G1095900.v1.1

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0000564
Physical Location & Seq
Forward (+)
6920921 .. 6924320
3400 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1095900.v1.1.491

Sequence Viewer

Length: 2457 bp
ATGGATACCAAACCAAACTCAAAGTTCGTGTTCTCCGTTATTTTAGTGTGTGTATTTCTCGAGTCCCATGTTTCCCTAGCAGCTGACACTATCAGTGAAAACCAATCTCTCTCCGGCGATCAAACCATTGCTTCTGCTGGTGGGATGTTTGAGCTAGGTTTCTTCAAACCAGGTCAGCTTTCAAACTACTATATAGGCATATGGTACTCCAAGCAAGTAGTATCTAAGATGACTGTTGTTTGGGTAGCAAATAGGGAAATACCAGTTTCTGATATATTTTCTTCGGCATTGAGCATCTTAGATGGTAACTTAGTTCTGTTTGACGAGTCCAAAACCCCGGTTTGGTCAACAAATTTAAACTCCACCACCAATTCTGGTTCTGTACAAGCAGTTCTTTTGGACAGCGGAAACCTTGTCCTGAAAGCGGGCTCTAGTAATAATACACTAGAGCCTTTATGGCAAAGCTTTGATCATCCAACTGATACTTTGCTACCAGGAGGTAAATTTGGATTCAACAAAATTACAAATCATTCCCAAATTCTGACTTCATGGAAGAATTTGGAGGATCCTGCACCAGGTCTTTACTCTCTTCACCTAGCCCCAGATGAAGGCGATTCATGTATCTATTTGTGGAATAGGTCTAGACAGTATTGGACCAGCGGACCATGGAATGAAAGTTTGCGTAATTTCAACATGGTTCCTGAATTGAGGCTATTTAATGACTTCAATTATAGTTCTGTTAGGAATGAGAACGAAAGTTATCTCACATATTCTATTAACAATCCTAAAATTGTAACTCGAAGCTTGATGACTGCCTCGGGACAGGTTCAGCAACTAACATGGTTGGAGGCTTCCAAGCAGTGGAATGTGTTTTGGTCTCAACCAAGACAGTGTGATGTTTATGCTTTTTGTGGGGCTTTCTCCAGTTGCAAACCTACATCCTTGAACTACTGCAAATGTTTGAAGGGCTTTGAGCCAAACCGGCAGAGTGATTGGGACTTGCAGATTTATTCTGGTGGATGTTCAAGAAGAACCAGTGTGAAGTGTGGGAATGCTACGGGAGACGGGTTTCTAGAAAAAAATAGCAAGTCCTTGCTTGAAAATAGCCAAAATGAACGCGCTCTTAGTTTTGAAAGTTGTAGATCAGCTTGCTTAAATGAATGCCAGTGCACTGCTTATGTTTATGACAGCAGCAATGGATGTTCAATATGGCATGGAGATGTCTTGAATCTGCAAGAATTTGAAGCAAATGATGGTGATGGAAGAATTTTATACATCAGAGCTGCAGCTTCCGACATTGAGAAAAAACGTGTAATTAAGTTGTCCCCTTTGATTGCAATAGTCTCAACAATCACAGGTTTGCTTGTTGTCATTTTTGGCTATTTCTTATGGAAGAAAACATTGGGAACAAAAAGTAAAGTTGCGGCTGGAGGTGAAATGAATGATGCAGAACTGCCGATCTTTGGTTTAAGGGCTATAATAGCAGCTACAAACAACTTCGCTGAAGCTAATAAACTCGGAGAGGGAGGATTTGGCCCTGTCTACAAGGGAATTTTGGCTGAAAATCAAGAAGTAGCCATAAAAAGGTTATCAAAAAAGTCAGGCCAAGGACACCAGGAGTTCATGAATGAGTTAAAACTTATTGCCAAGCTCCAGCATACCAATCTCGTTAGGCTCCTGGGTTGCTGTATTGAAGACGAGGAAATGATATTGATCTACGAGTACATGCCCAATCGGAGCTTGGACAAACTTTTGTTTGATGCACCTGAAAAAAGAGAATTAGATTGGGGAAGACGTTTTCGAATTATAGAAGGCATTGCTCAAGGAGTACTTTATATCCACAAGTACTCGAGATTGAAAATCATCCACAGGGACCTAAAAACAAGTAACATATTGTTGGATGGAGTCTTGAACCCCAAAATTTCAGACTTTGGAATGGCAAGGATTTTCGGGATAAATCAGACTGAAGCGAATACCAATAGGGTTGTTGGCACATACGGCTACATGTCACCCGAGTATGCGAGATATGGTCATTTCTCTGAGAAATTAGATGTATTCAGCTTCGGAGTGGTGTTGTTGGAGATTGTCAGCGGAAAGAAGAATGCTGCTTTTTATCTCTTTGAACATTCACCAACTCTTGCTGGATGGGCATGGGACTTGTGGAAAGACGGAAGAGGAATGGAGGTGATTGATGAATCGGTAAGGGAAACATGTCGGCTCGATGAAGCTTTGAAGTGTATCCATGTAGGGTTTTTGTGTGTTCAAGAAGCTCCAGCTGATCGACCAACAATGTCTTTAGTAATTCGTATGCTGCAGGGCAATGAATCTACATCTCTTCCACCTTTCAAAGAACCTGCCTTTTCAACACACAGAAATTCCAGTCCTGTTTGTTCTTCTCAAACACCTACCATTTTTTCCCACAACACAGTCACCATGAGTTTGCCAGAAGGTCGATAG

Protein Analysis

819

Amino Acids

91.43

Weight (kDa)

6.22

Isoelectric Point (pI)

42.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 78 - 185 1.9e-33 D-mannose binding lectin
S_locus_glycop PF00954 218 - 327 3.1e-27 S-locus glycoprotein domain
PAN_2 PF08276 349 - 410 3.6e-11 PAN-like domain
Pkinase PF00069 501 - 700 1.4e-46 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 504 - 770 1.2e-47 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000564)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g44064 FvH4_6g44064 FvH4_6g44100 FvH4_6g44101 FvH4_6g51830
malus_domestica MD09G1094400.v1.1 MD09G1094800.v1.1 MD09G1095200.v1.1 MD09G1095900.v1.1 MD09G1096000.v1.1 MD09G1096200.v1.1 MD17G1083900.v1.1 MD17G1088500.v1.1
prunus_persica Prupe.3G224900_v2.0.a1 Prupe.3G224900_v2.0.a1 Prupe.3G224900_v2.0.a1 Prupe.3G224900_v2.0.a1 Prupe.3G224900_v2.0.a1 Prupe.3G224900_v2.0.a1 Prupe.3G225100_v2.0.a1 Prupe.3G225100_v2.0.a1 Prupe.3G225100_v2.0.a1 Prupe.3G225200_v2.0.a1 Prupe.3G225200_v2.0.a1
pyrus_communis pycom09g01920 pycom09g01930 pycom09g01940 pycom09g01950 pycom09g02000 pycom09g02050 pycom09g02070 pycom17g08540
rosa_chinensis RchiOBHm_Chr1g0318601 RchiOBHm_Chr1g0318761 RchiOBHm_Chr1g0342541 RchiOBHm_Chr2g0160651 RchiOBHm_Chr2g0160831 RchiOBHm_Chr4g0405661 RchiOBHm_Chr4g0405691 RchiOBHm_Chr4g0405701 RchiOBHm_Chr7g0238591
rosa_laevigata RLG00000002292 RLG00000008826 RLG00000008828 RLG00000008829 RLG00000021243 RLG00000021245 RLG00000030522
rosa_multiflora Rmu_co8432573.1_g000001 Rmu_sc0001200.1_g000041 Rmu_sc0001200.1_g000043 Rmu_sc0002545.1_g000017 Rmu_sc0002773.1_g000017 Rmu_sc0012550.1_g000008
rosa_roxburghii Rroxscaffold_2G00089500 Rroxscaffold_5G00350130 Rroxscaffold_5G00350180
rosa_rugosa Rorug01G0022300 Rorug04G0061600
rosa_samantha Rh1AG035300 Rh1CG034300 Rh1DG036100 Rh1DG149100 Rh2AG550900 Rh2BG564200 Rh2BG565500 Rh2CG535300 Rh2DG574400 Rh4AG133100 Rh4AG133200 Rh4AG133300 Rh4BG128200 Rh4CG141000 Rh4DG127700 Rh4DG127900
rosa_wichuraiana Rw2G045540 Rw2G045650 Rw4G010790 Rw4G010800 Rw4G010810 Rw4G010830 Rw7G027620 Rw7G027630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 2362
AccB7I CCANNNNNTGG 1 cut(s) 861
AccI GTMKAC 1 cut(s) 1542
AccII CGCG 1 cut(s) 1119
AciI CCGC 5 cut(s) 405, 425, 660, 1424, 2091
AclWI GGATC 2 cut(s) 560, 573
AcsI RAATTY 9 cut(s) 352, 503, 537, 556, 1238, 1266, 1551, 1920, 2374
AcuI CTGAAG 2 cut(s) 1524, 1986
AfaI GTAC 5 cut(s) 206, 384, 1724, 1830, 1847
AfiI CCNNNNNNNGG 7 cut(s) 342, 424, 575, 608, 861, 1463, 1584
AflIII ACRYGT 3 cut(s) 1309, 2004, 2210
AjnI CCWGG 5 cut(s) 169, 493, 574, 1614, 1677
AjuI GAANNNNNNNTTGG 2 cut(s) 1385, 1417
Alw21I GWGCWC 1 cut(s) 1172
Alw26I GTCTC 3 cut(s) 882, 1056, 1348
Alw44I GTGCAC 1 cut(s) 1168
AlwI GGATC 2 cut(s) 560, 573
AlwNI CAGNNNCTG 1 cut(s) 269
Ama87I CYCGRG 4 cut(s) 59, 817, 1849, 2012
AoxI GGCC 2 cut(s) 1534, 1603
ApaLI GTGCAC 1 cut(s) 1168
ApeKI GCWGC 7 cut(s) 80, 1191, 1283, 1286, 1484, 2105, 2311
ApoI RAATTY 9 cut(s) 352, 503, 537, 556, 1238, 1266, 1551, 1920, 2374
AspLEI GCGC 1 cut(s) 1121
AspS9I GGNCC 4 cut(s) 654, 662, 1535, 1873
AsuC2I CCSGG 1 cut(s) 338
AsuHPI GGTGA 7 cut(s) 584, 1268, 1445, 2001, 2121, 2197, 2422
AsuII TTCGAA 1 cut(s) 1801
AvaI CYCGRG 4 cut(s) 59, 817, 1849, 2012
AvaII GGWCC 3 cut(s) 654, 662, 1873
BaeGI GKGCMC 1 cut(s) 1172
BamHI GGATCC 1 cut(s) 565
BanII GRGCYC 1 cut(s) 431
BbsI GAAGAC 2 cut(s) 1701, 1798
Bbv12I GWGCWC 1 cut(s) 1172
BbvI GCAGC 7 cut(s) 92, 1203, 1270, 1298, 1496, 2092, 2298
BccI CCATC 5 cut(s) 296, 1247, 1253, 1895, 2139
BceAI ACGGC 1 cut(s) 2014
BciT130I CCWGG 5 cut(s) 171, 495, 576, 1616, 1679
BciVI GTATCC 1 cut(s) 2249
BclI TGATCA 1 cut(s) 469
BcnI CCSGG 1 cut(s) 338
BcoDI GTCTC 3 cut(s) 882, 1056, 1348
BfaI CTAG 7 cut(s) 77, 155, 432, 446, 596, 642, 1073
BfmI CTRYAG 2 cut(s) 1284, 2312
BfuAI ACCTGC 1 cut(s) 2362
BfuI GTATCC 1 cut(s) 2249
BglI GCCNNNNNGGC 2 cut(s) 457, 982
BisI GCNGC 8 cut(s) 81, 1192, 1284, 1287, 1425, 1485, 2106, 2312
BlsI GCNGC 8 cut(s) 82, 1193, 1285, 1288, 1426, 1486, 2107, 2313
BmcAI AGTACT 2 cut(s) 1830, 1847
Bme1390I CCNGG 6 cut(s) 171, 338, 495, 576, 1616, 1679
Bme18I GGWCC 3 cut(s) 654, 662, 1873
BmeT110I CYCGRG 4 cut(s) 59, 817, 1849, 2012
BmgT120I GGNCC 4 cut(s) 654, 662, 1535, 1873
BmiI GGNNCC 4 cut(s) 567, 699, 1676, 1874
BmrFI CCNGG 6 cut(s) 171, 338, 495, 576, 1616, 1679
BmsI GCATC 3 cut(s) 303, 1435, 1750
BpiI GAAGAC 2 cut(s) 1701, 1798
BpmI CTGGAG 4 cut(s) 907, 1449, 1637, 2256
Bpu14I TTCGAA 1 cut(s) 1801
BpuEI CTTGAG 1 cut(s) 1806
BpuMI CCSGG 1 cut(s) 338
BsaBI GATNNNNATC 1 cut(s) 1712
BsaI GGTCTC 1 cut(s) 882
BsaJI CCNNGG 5 cut(s) 336, 665, 816, 1606, 1678
Bsc4I CCNNNNNNNGG 7 cut(s) 342, 424, 575, 608, 861, 1463, 1584
Bse118I RCCGGY 1 cut(s) 981
Bse1I ACTGG 5 cut(s) 263, 924, 1035, 1165, 2379
Bse3DI GCAATG 4 cut(s) 126, 1201, 1815, 2326
Bse8I GATNNNNATC 1 cut(s) 1712
BseBI CCWGG 5 cut(s) 171, 495, 576, 1616, 1679
BseDI CCNNGG 5 cut(s) 336, 665, 816, 1606, 1678
BseGI GGATG 8 cut(s) 150, 472, 938, 1025, 1205, 1863, 1906, 2150
BseJI GATNNNNATC 1 cut(s) 1712
BseLI CCNNNNNNNGG 7 cut(s) 342, 424, 575, 608, 861, 1463, 1584
BseMI GCAATG 4 cut(s) 126, 1201, 1815, 2326
BseMII CTCAG 1 cut(s) 2031
BseNI ACTGG 5 cut(s) 263, 924, 1035, 1165, 2379
BseSI GKGCMC 1 cut(s) 1172
BseXI GCAGC 7 cut(s) 92, 1203, 1270, 1298, 1496, 2092, 2298
BsgI GTGCAG 1 cut(s) 555
Bsh1236I CGCG 1 cut(s) 1119
BshFI GGCC 2 cut(s) 1536, 1605
BsiHKAI GWGCWC 1 cut(s) 1172
BsiHKCI CYCGRG 4 cut(s) 59, 817, 1849, 2012
BsiSI CCGG 3 cut(s) 114, 338, 982
BslFI GGGAC 6 cut(s) 49, 834, 1010, 1309, 1886, 2168
BslI CCNNNNNNNGG 7 cut(s) 342, 424, 575, 608, 861, 1463, 1584
BsmAI GTCTC 3 cut(s) 882, 1056, 1348
BsmBI CGTCTC 1 cut(s) 1056
BsmFI GGGAC 6 cut(s) 49, 834, 1010, 1309, 1886, 2168
BsmI GAATGC 3 cut(s) 1057, 1166, 2107
BsnI GGCC 2 cut(s) 1536, 1605
Bso31I GGTCTC 1 cut(s) 882
BsoBI CYCGRG 4 cut(s) 59, 817, 1849, 2012
Bsp119I TTCGAA 1 cut(s) 1801
Bsp1286I GDGCHC 2 cut(s) 431, 1172
Bsp1407I TGTACA 1 cut(s) 382
Bsp143I GATC 7 cut(s) 118, 469, 565, 1142, 1458, 1713, 2278
Bsp19I CCATGG 1 cut(s) 665
BspACI CCGC 5 cut(s) 405, 425, 660, 1424, 2091
BspANI GGCC 2 cut(s) 1536, 1605
BspCNI CTCAG 1 cut(s) 2032
BspFNI CGCG 1 cut(s) 1119
BspHI TCATGA 1 cut(s) 1623
BspLI GGNNCC 4 cut(s) 567, 699, 1676, 1874
BspMAI CTGCAG 2 cut(s) 1288, 2316
BspMI ACCTGC 1 cut(s) 2362
BspPI GGATC 2 cut(s) 560, 573
BspT104I TTCGAA 1 cut(s) 1801
BspTNI GGTCTC 1 cut(s) 882
BsrDI GCAATG 4 cut(s) 126, 1201, 1815, 2326
BsrFI RCCGGY 1 cut(s) 981
BsrGI TGTACA 1 cut(s) 382
BsrI ACTGG 5 cut(s) 263, 924, 1035, 1165, 2379
BssAI RCCGGY 1 cut(s) 981
BssECI CCNNGG 5 cut(s) 336, 665, 816, 1606, 1678
BssMI GATC 7 cut(s) 118, 469, 565, 1142, 1458, 1713, 2278
BssT1I CCWWGG 2 cut(s) 665, 1606
Bst2UI CCWGG 5 cut(s) 171, 495, 576, 1616, 1679
Bst4CI ACNGT 4 cut(s) 235, 648, 891, 2428
Bst6I CTCTTC 3 cut(s) 594, 2167, 2340
BstAUI TGTACA 1 cut(s) 382
BstBI TTCGAA 1 cut(s) 1801
BstC8I GCNNGC 2 cut(s) 427, 1150
BstDEI CTNAG 5 cut(s) 225, 298, 310, 1124, 2040
BstDSI CCRYGG 1 cut(s) 665
BstENI CCTNNNNNAGG 1 cut(s) 573
BstF5I GGATG 8 cut(s) 150, 472, 938, 1025, 1205, 1863, 1906, 2150
BstFNI CGCG 1 cut(s) 1119
BstHHI GCGC 1 cut(s) 1121
BstKTI GATC 7 cut(s) 121, 472, 568, 1145, 1461, 1716, 2281
BstMAI GTCTC 3 cut(s) 882, 1056, 1348
BstMBI GATC 7 cut(s) 118, 469, 565, 1142, 1458, 1713, 2278
BstMWI GCNNNNNNNGC 5 cut(s) 457, 982, 1481, 1998, 2147
BstNI CCWGG 5 cut(s) 171, 495, 576, 1616, 1679
BstNSI RCATGY 3 cut(s) 1729, 2008, 2214
BstSCI CCNGG 6 cut(s) 169, 336, 493, 574, 1614, 1677
BstSFI CTRYAG 2 cut(s) 1284, 2312
BstSLI GKGCMC 1 cut(s) 1172
BstUI CGCG 1 cut(s) 1119
BstV1I GCAGC 7 cut(s) 92, 1203, 1270, 1298, 1496, 2092, 2298
BstV2I GAAGAC 2 cut(s) 1701, 1798
BstX2I RGATCY 1 cut(s) 565
BstYI RGATCY 1 cut(s) 565
BsuI GTATCC 1 cut(s) 2249
BsuRI GGCC 2 cut(s) 1536, 1605
BtgI CCRYGG 1 cut(s) 665
BtsCI GGATG 8 cut(s) 150, 472, 938, 1025, 1205, 1863, 1906, 2150
BtsI GCAGTG 2 cut(s) 866, 1170
BtsIMutI CAGTG 6 cut(s) 100, 866, 896, 1042, 1170, 1172
BveI ACCTGC 1 cut(s) 2362
Cac8I GCNNGC 2 cut(s) 427, 1150
CaiI CAGNNNCTG 1 cut(s) 269
CciI TCATGA 1 cut(s) 1623
CfoI GCGC 1 cut(s) 1121
Cfr10I RCCGGY 1 cut(s) 981
Cfr13I GGNCC 4 cut(s) 654, 662, 1535, 1873
CsiI ACCWGGT 2 cut(s) 169, 574
Csp6I GTAC 5 cut(s) 205, 383, 1723, 1829, 1846
CviQI GTAC 5 cut(s) 205, 383, 1723, 1829, 1846
DdeI CTNAG 5 cut(s) 225, 298, 310, 1124, 2040
DpnI GATC 7 cut(s) 120, 471, 567, 1144, 1460, 1715, 2280
DpnII GATC 7 cut(s) 118, 469, 565, 1142, 1458, 1713, 2278
DraI TTTAAA 1 cut(s) 357
Eam1104I CTCTTC 3 cut(s) 594, 2167, 2340
EarI CTCTTC 3 cut(s) 594, 2167, 2340
Eco130I CCWWGG 2 cut(s) 665, 1606
Eco24I GRGCYC 1 cut(s) 431
Eco31I GGTCTC 1 cut(s) 882
Eco47I GGWCC 3 cut(s) 654, 662, 1873
Eco57I CTGAAG 2 cut(s) 1524, 1986
Eco88I CYCGRG 4 cut(s) 59, 817, 1849, 2012
EcoNI CCTNNNNNAGG 1 cut(s) 573
EcoO109I RGGNCCY 1 cut(s) 1873
EcoRII CCWGG 5 cut(s) 169, 493, 574, 1614, 1677
EcoT14I CCWWGG 2 cut(s) 665, 1606
EcoT38I GRGCYC 1 cut(s) 431
ErhI CCWWGG 2 cut(s) 665, 1606
Esp3I CGTCTC 1 cut(s) 1056
FalI AAGNNNNNCTT 4 cut(s) 378, 410, 1815, 1847
FaqI GGGAC 6 cut(s) 49, 834, 1010, 1309, 1886, 2168
FauI CCCGC 1 cut(s) 418
FauNDI CATATG 1 cut(s) 200
FbaI TGATCA 1 cut(s) 469
FblI GTMKAC 1 cut(s) 1542
Fnu4HI GCNGC 8 cut(s) 81, 1192, 1284, 1287, 1425, 1485, 2106, 2312
FokI GGATG 8 cut(s) 157, 459, 925, 1032, 1212, 1850, 1913, 2157
FriOI GRGCYC 1 cut(s) 431
Fsp4HI GCNGC 8 cut(s) 81, 1192, 1284, 1287, 1425, 1485, 2106, 2312
FspBI CTAG 7 cut(s) 77, 155, 432, 446, 596, 642, 1073
GlaI GCGC 1 cut(s) 1120
GluI GCNGC 8 cut(s) 81, 1192, 1284, 1287, 1425, 1485, 2106, 2312
GsuI CTGGAG 4 cut(s) 907, 1449, 1637, 2256
HaeIII GGCC 2 cut(s) 1536, 1605
HapII CCGG 3 cut(s) 114, 338, 982
HhaI GCGC 1 cut(s) 1121
Hin6I GCGC 1 cut(s) 1119
HinP1I GCGC 1 cut(s) 1119
HincII GTYRAC 1 cut(s) 348
HindII GTYRAC 1 cut(s) 348
HindIII AAGCTT 3 cut(s) 463, 802, 2226
HinfI GANTC 8 cut(s) 62, 326, 510, 614, 1228, 1905, 2195, 2324
HpaII CCGG 3 cut(s) 114, 338, 982
HphI GGTGA 7 cut(s) 584, 1268, 1445, 2001, 2121, 2197, 2422
Hpy166II GTNNAC 3 cut(s) 348, 1170, 1543
Hpy8I GTNNAC 3 cut(s) 348, 1170, 1543
HpyAV CCTTC 4 cut(s) 602, 958, 1805, 2441
HpyCH4III ACNGT 4 cut(s) 235, 648, 891, 2428
HpyCH4IV ACGT 2 cut(s) 1309, 1795
HpyF10VI GCNNNNNNNGC 5 cut(s) 457, 982, 1481, 1998, 2147
HpyF3I CTNAG 5 cut(s) 225, 298, 310, 1124, 2040
HpySE526I ACGT 2 cut(s) 1309, 1795
HspAI GCGC 1 cut(s) 1119
Ksp22I TGATCA 1 cut(s) 469
Kzo9I GATC 7 cut(s) 118, 469, 565, 1142, 1458, 1713, 2278
LmnI GCTCC 4 cut(s) 1656, 1680, 1737, 2275
Lsp1109I GCAGC 7 cut(s) 92, 1203, 1270, 1298, 1496, 2092, 2298
LweI GCATC 3 cut(s) 303, 1435, 1750
MabI ACCWGGT 2 cut(s) 169, 574
MaeI CTAG 7 cut(s) 77, 155, 432, 446, 596, 642, 1073
MaeII ACGT 2 cut(s) 1309, 1795
MaeIII GTNAC 5 cut(s) 305, 793, 1886, 2007, 2428
MalI GATC 7 cut(s) 120, 471, 567, 1144, 1460, 1715, 2280
MboI GATC 7 cut(s) 118, 469, 565, 1142, 1458, 1713, 2278
MflI RGATCY 1 cut(s) 565
MhlI GDGCHC 2 cut(s) 431, 1172
MlyI GAGTC 3 cut(s) 71, 335, 1914
MmeI TCCRAC 5 cut(s) 500, 825, 1317, 1878, 2058
MseI TTAA 7 cut(s) 356, 717, 777, 1154, 1317, 1469, 1634
MslI CAYNNNNRTG 1 cut(s) 1218
MspA1I CMGCKG 5 cut(s) 83, 405, 660, 2091, 2276
MspI CCGG 3 cut(s) 114, 338, 982
MspR9I CCNGG 6 cut(s) 171, 338, 495, 576, 1616, 1679
Mva1269I GAATGC 3 cut(s) 1057, 1166, 2107
MvaI CCWGG 5 cut(s) 171, 495, 576, 1616, 1679
MvnI CGCG 1 cut(s) 1119
MwoI GCNNNNNNNGC 5 cut(s) 457, 982, 1481, 1998, 2147
NciI CCSGG 1 cut(s) 338
NcoI CCATGG 1 cut(s) 665
NdeI CATATG 1 cut(s) 200
NdeII GATC 7 cut(s) 118, 469, 565, 1142, 1458, 1713, 2278
NlaIV GGNNCC 4 cut(s) 567, 699, 1676, 1874
NmuCI GTSAC 2 cut(s) 2007, 2428
NspI RCATGY 3 cut(s) 1729, 2008, 2214
NspV TTCGAA 1 cut(s) 1801
PaeR7I CTCGAG 2 cut(s) 59, 1849
PagI TCATGA 1 cut(s) 1623
PciI ACATGT 2 cut(s) 2004, 2210
PcsI WCGNNNNNNNCGW 1 cut(s) 33
PctI GAATGC 3 cut(s) 1057, 1166, 2107
PfeI GAWTC 5 cut(s) 510, 614, 1228, 2195, 2324
PflMI CCANNNNNTGG 1 cut(s) 861
PkrI GCNGC 8 cut(s) 82, 1193, 1285, 1288, 1426, 1486, 2107, 2313
PleI GAGTC 3 cut(s) 70, 334, 1913
PpsI GAGTC 3 cut(s) 70, 334, 1913
PpuMI RGGWCCY 1 cut(s) 1873
PscI ACATGT 2 cut(s) 2004, 2210
Psp5II RGGWCCY 1 cut(s) 1873
Psp6I CCWGG 5 cut(s) 169, 493, 574, 1614, 1677
PspGI CCWGG 5 cut(s) 169, 493, 574, 1614, 1677
PspN4I GGNNCC 4 cut(s) 567, 699, 1676, 1874
PspPI GGNCC 4 cut(s) 654, 662, 1535, 1873
PspPPI RGGWCCY 1 cut(s) 1873
PsrI GAACNNNNNNTAC 2 cut(s) 375, 407
PstI CTGCAG 2 cut(s) 1288, 2316
PstNI CAGNNNCTG 1 cut(s) 269
PsuI RGATCY 1 cut(s) 565
PvuII CAGCTG 2 cut(s) 83, 2276
RsaI GTAC 5 cut(s) 206, 384, 1724, 1830, 1847
RsaNI GTAC 5 cut(s) 205, 383, 1723, 1829, 1846
RseI CAYNNNNRTG 1 cut(s) 1218
SaqAI TTAA 7 cut(s) 356, 717, 777, 1154, 1317, 1469, 1634
SatI GCNGC 8 cut(s) 81, 1192, 1284, 1287, 1425, 1485, 2106, 2312
Sau3AI GATC 7 cut(s) 118, 469, 565, 1142, 1458, 1713, 2278
Sau96I GGNCC 4 cut(s) 654, 662, 1535, 1873
ScaI AGTACT 2 cut(s) 1830, 1847
SchI GAGTC 3 cut(s) 71, 335, 1914
ScrFI CCNGG 6 cut(s) 171, 338, 495, 576, 1616, 1679
SduI GDGCHC 2 cut(s) 431, 1172
SexAI ACCWGGT 2 cut(s) 169, 574
SfaNI GCATC 3 cut(s) 303, 1435, 1750
SfcI CTRYAG 2 cut(s) 1284, 2312
Sfr274I CTCGAG 2 cut(s) 59, 1849
SfuI TTCGAA 1 cut(s) 1801
SinI GGWCC 3 cut(s) 654, 662, 1873
SlaI CTCGAG 2 cut(s) 59, 1849
SmiMI CAYNNNNRTG 1 cut(s) 1218
SmlI CTYRAG 3 cut(s) 59, 1821, 1849
SmoI CTYRAG 3 cut(s) 59, 1821, 1849
SsiI CCGC 5 cut(s) 405, 425, 660, 1424, 2091
SspMI CTAG 7 cut(s) 77, 155, 432, 446, 596, 642, 1073
StyD4I CCNGG 6 cut(s) 169, 336, 493, 574, 1614, 1677
StyI CCWWGG 2 cut(s) 665, 1606
TaaI ACNGT 4 cut(s) 235, 648, 891, 2428
TaiI ACGT 2 cut(s) 1312, 1798
TaqI TCGA 7 cut(s) 60, 799, 1801, 1850, 2220, 2281, 2452
TatI WGTACW 4 cut(s) 382, 1722, 1828, 1845
TauI GCSGC 1 cut(s) 1427
TfiI GAWTC 5 cut(s) 510, 614, 1228, 2195, 2324
Tru1I TTAA 7 cut(s) 356, 717, 777, 1154, 1317, 1469, 1634
Tru9I TTAA 7 cut(s) 356, 717, 777, 1154, 1317, 1469, 1634
TscAI CASTG 6 cut(s) 100, 866, 896, 1042, 1172, 1177
TseFI GTSAC 2 cut(s) 2007, 2428
TseI GCWGC 7 cut(s) 80, 1191, 1283, 1286, 1484, 2105, 2311
Tsp45I GTSAC 2 cut(s) 2007, 2428
TspGWI ACGGA 2 cut(s) 25, 2184
TspRI CASTG 6 cut(s) 100, 866, 896, 1042, 1172, 1177
Van91I CCANNNNNTGG 1 cut(s) 861
VneI GTGCAC 1 cut(s) 1168
VpaK11BI GGWCC 3 cut(s) 654, 662, 1873
XagI CCTNNNNNAGG 1 cut(s) 573
XapI RAATTY 9 cut(s) 352, 503, 537, 556, 1238, 1266, 1551, 1920, 2374
XbaI TCTAGA 2 cut(s) 641, 1072
XceI RCATGY 3 cut(s) 1729, 2008, 2214
XcmI CCANNNNNNNNNTGG 1 cut(s) 1738
XhoI CTCGAG 2 cut(s) 59, 1849
XmiI GTMKAC 1 cut(s) 1542
XspI CTAG 7 cut(s) 77, 155, 432, 446, 596, 642, 1073
ZrmI AGTACT 2 cut(s) 1830, 1847
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.