MD09G1096200.v1.1

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0000564
Physical Location & Seq
Forward (+)
6937429 .. 6940491
3063 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1096200.v1.1.491

Sequence Viewer

Length: 2277 bp
ATGAATACCAAACCAAACTCAAAGTTCGTGTTCTCCGTTATTTTATTATGTGCATTTCTCGAGTACCATGTTTACCTAGCAGCTGCCACGATCAGCGAAAACCATTCTCTCTCGGGCAATCAAACCATTGTCTCCCCAGGTAGGATTTTTGAGCTCGGTTTCTTCAAACCAGGTAAACTTTCAAACTTCTACATAGGCATGTGGTACACCGACCGAGTAGTATCCGAGAGGACCATTGTTTGGGTGGCAAACAGGGAAAACCCAGTTTCCGATATATTTTCTTCATCCAAAGCGATTGTTTGGTCAACGAATTTAACCTCCTCCTCCTCCGCCACTGCATGTCTTTTGGATACCGGAAACCTGGTCTTGAGAGCCGAGTCTAGTAATAGTTCAGAGCCGTTATGGCAGAGCTTTGATCATCCAACGCATACTTGGCTGCCAGGAGGTAGAATTAGGTTTAACACTGTTACCAAACAAACCCAAATTCTTACTTCATGGAAGAGTTCGGAGGATCCTGCACCAGGTCTTTTCTCGCTCGAGTTAGACCCGAACGGAAGCAGTGCGTTTTTCATAATGTGGAATAGGTCTAGGCAGAATTGGACTAGTGGATCATGGGATGCAAAATTAAGGGTTTTCCGCTTGGTAAGTACGGGGCTCAATAAAAAAATATACAACCTCAGCTATGTTACCAACGCAACCGAAGGTTATTTCACCCATTCTGTTTATGATCCTAGGAGAATATCTCGATTCGTGTTGCTTACTTCGGGCCAGATTCAACAGCTGACATGGATTGAGAATTCCACGCAATGGAATTTGGTTTGGTCTCAACCGAGAAAGCAATGTGAAGTTTATGATTTGTGTGGGGCCTTCGGAAGTTGCAATGAGGAAGGTCCCCTCGCCTGTAATTGCTTGAAAGGTTTTGAGCCGAAGGTGATGAGAGATTGGGATTTAAAGGACTACTCTGGTGGGTGTTCGAGAAAAACCGATCTGCATTGTGGAAATGCTACTACTGCGAGCAGAAAGCCAGACCGGTTTCTTGAACTTCCTAGCATGTCATTGCCTAAAAATAATTACACTATGGAGGTTGGGAGTATTGCGGAATGTGAACCGTTCTGCCTTAGAAGCTGTTCTTGCACTGCTTTTGCTTATGACAGCAGCGGATGTTCGATTTGGAACGGAGAACTCTTGGATTTGCAACAACCAACATCAAGTGACAAACAAGGAAGAACTTTATACCTCAGACTTGCAGCTTCTGAGTTTGAGAGTTCTCAAAGTAACAAGGGATTGAATGTTGGTGTTGTGATTGCAATTGTCTCAATAATCACAGGTTTGCTGGTTGTCATTTTTGGCTATTACTTACGGAAGAAAACATTGGAAAAGAAAAGAGAGCATAGGAAAAGAAATGGTGAAAAGAACGATGCAGAACTGCCTATCTTTGGTTTAAGGGCTATAATAGCTGCTACAAACAATTTCGCCGAAGCTAATAAACTCGGAGAGGGAGGATTTGGCCCTGTCTACAAGGGAATTTTGGGTGAAAATCAAGAAGTAGCCATAAAAAGGTTATCAAAAAAGTCAGGCCAAGGACAACAGGAGTTCATGAATGAGTTAAAACTTATTGCTAAGCTCCAACATACCAATCTCGTTAGGCTATTGGGTTGCTGTCTTGAAGACGAGGAAATGATATTGATCTACGAGTACATGCCCAATCGGAGTTTGGACAAACTTTTGTTTGATGCATCTGAAAAAGGAGAACTAGATTGGGGAAGACGTTTTCAAATTATAGACGGCATTGCTCAAGGAGTACTTTATATCCACAACGGTTACATGTTACCCGAATATGCGAGATATGGTCATTTCTCTGAGAAATTAGATGTATTTAGCTTTGGAGTGTTGTTATTGGAGATTGTAAGCGGAAAGAAGAATGCTGATTTTTATCGCTTTGAACATTCATCGACTCTTGCTGGATGGGCATGGGAGATATGGAAAGACGGAAGAGGAACGAAGGTGATTGATGAATCAGTAAGGGAAACATGCCTGCTTGATGAAGCTTTGAAGTGCATCCATGTAGGGTTTTTGTGTGTTCAAGAAGCTCCAGCTGATCAACCAACAATGTCTTCAATAATTCGTATGCTGCAGGGCAATGAATCTACATCTCTTCCATCTTCCAAAGAACCTGCCTTTTCAACACACAGAAATTCCAGTCATGTTCACTCTTCTGAAACGCCTACCATTTTTTCCCAAAACACAGTCACCATGAGTTTGCCAGAAGGTCGATAG

Protein Analysis

759

Amino Acids

85.11

Weight (kDa)

7.19

Isoelectric Point (pI)

42.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 78 - 167 1.1e-21 D-mannose binding lectin
S_locus_glycop PF00954 200 - 310 3.4e-28 S-locus glycoprotein domain
PAN_2 PF08276 340 - 398 1.5e-14 PAN-like domain
Pkinase PF00069 492 - 606 6.7e-19 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 495 - 606 4.7e-22 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000564)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g44064 FvH4_6g44064 FvH4_6g44100 FvH4_6g44101 FvH4_6g51830
malus_domestica MD09G1094400.v1.1 MD09G1094800.v1.1 MD09G1095200.v1.1 MD09G1095900.v1.1 MD09G1096000.v1.1 MD09G1096200.v1.1 MD17G1083900.v1.1 MD17G1088500.v1.1
prunus_persica Prupe.3G224900_v2.0.a1 Prupe.3G224900_v2.0.a1 Prupe.3G224900_v2.0.a1 Prupe.3G224900_v2.0.a1 Prupe.3G224900_v2.0.a1 Prupe.3G224900_v2.0.a1 Prupe.3G225100_v2.0.a1 Prupe.3G225100_v2.0.a1 Prupe.3G225100_v2.0.a1 Prupe.3G225200_v2.0.a1 Prupe.3G225200_v2.0.a1
pyrus_communis pycom09g01920 pycom09g01930 pycom09g01940 pycom09g01950 pycom09g02000 pycom09g02050 pycom09g02070 pycom17g08540
rosa_chinensis RchiOBHm_Chr1g0318601 RchiOBHm_Chr1g0318761 RchiOBHm_Chr1g0342541 RchiOBHm_Chr2g0160651 RchiOBHm_Chr2g0160831 RchiOBHm_Chr4g0405661 RchiOBHm_Chr4g0405691 RchiOBHm_Chr4g0405701 RchiOBHm_Chr7g0238591
rosa_laevigata RLG00000002292 RLG00000008826 RLG00000008828 RLG00000008829 RLG00000021243 RLG00000021245 RLG00000030522
rosa_multiflora Rmu_co8432573.1_g000001 Rmu_sc0001200.1_g000041 Rmu_sc0001200.1_g000043 Rmu_sc0002545.1_g000017 Rmu_sc0002773.1_g000017 Rmu_sc0012550.1_g000008
rosa_roxburghii Rroxscaffold_2G00089500 Rroxscaffold_5G00350130 Rroxscaffold_5G00350180
rosa_rugosa Rorug01G0022300 Rorug04G0061600
rosa_samantha Rh1AG035300 Rh1CG034300 Rh1DG036100 Rh1DG149100 Rh2AG550900 Rh2BG564200 Rh2BG565500 Rh2CG535300 Rh2DG574400 Rh4AG133100 Rh4AG133200 Rh4AG133300 Rh4BG128200 Rh4CG141000 Rh4DG127700 Rh4DG127900
rosa_wichuraiana Rw2G045540 Rw2G045650 Rw4G010790 Rw4G010800 Rw4G010810 Rw4G010830 Rw7G027620 Rw7G027630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 2182
AccB7I CCANNNNNTGG 2 cut(s) 240, 807
AccI GTMKAC 1 cut(s) 1515
AciI CCGC 5 cut(s) 330, 637, 1097, 1158, 1911
AclWI GGATC 4 cut(s) 506, 519, 616, 722
AcsI RAATTY 6 cut(s) 310, 483, 796, 811, 1524, 2194
AfaI GTAC 5 cut(s) 65, 206, 649, 1697, 1803
AfiI CCNNNNNNNGG 6 cut(s) 141, 240, 521, 807, 1436, 1557
AflIII ACRYGT 1 cut(s) 1824
AgeI ACCGGT 1 cut(s) 1029
AhlI ACTAGT 1 cut(s) 602
AjnI CCWGG 5 cut(s) 136, 169, 360, 439, 520
AjuI GAANNNNNNNTTGG 4 cut(s) 1355, 1387, 2098, 2130
Alw21I GWGCWC 1 cut(s) 156
Alw26I GTCTC 3 cut(s) 136, 828, 1318
AlwI GGATC 4 cut(s) 506, 519, 616, 722
AlwNI CAGNNNCTG 1 cut(s) 1253
Ama87I CYCGRG 3 cut(s) 59, 112, 536
AoxI GGCC 4 cut(s) 766, 864, 1507, 1576
ApeKI GCWGC 7 cut(s) 80, 83, 436, 1155, 1247, 1457, 2131
ApoI RAATTY 6 cut(s) 310, 483, 796, 811, 1524, 2194
AsiGI ACCGGT 1 cut(s) 1029
Asp700I GAANNNNTTC 1 cut(s) 1126
AspA2I CCTAGG 1 cut(s) 731
AspS9I GGNCC 5 cut(s) 231, 766, 864, 890, 1508
AsuHPI GGTGA 6 cut(s) 703, 943, 1418, 1544, 2017, 2242
AvaI CYCGRG 3 cut(s) 59, 112, 536
AvaII GGWCC 2 cut(s) 231, 890
AvrII CCTAGG 1 cut(s) 731
BamHI GGATCC 1 cut(s) 511
BanII GRGCYC 2 cut(s) 156, 657
BbsI GAAGAC 3 cut(s) 1674, 1771, 2106
Bbv12I GWGCWC 1 cut(s) 156
BbvCI CCTCAGC 1 cut(s) 677
BbvI GCAGC 7 cut(s) 70, 92, 423, 1167, 1259, 1444, 2118
BccI CCATC 2 cut(s) 1959, 2167
BceAI ACGGC 2 cut(s) 382, 1801
BciT130I CCWGG 5 cut(s) 138, 171, 362, 441, 522
BciVI GTATCC 2 cut(s) 232, 343
BclI TGATCA 2 cut(s) 415, 2098
BcoDI GTCTC 3 cut(s) 136, 828, 1318
BcuI ACTAGT 1 cut(s) 602
BfaI CTAG 7 cut(s) 77, 381, 588, 603, 732, 1047, 1754
BfmI CTRYAG 1 cut(s) 2132
BfuAI ACCTGC 1 cut(s) 2182
BfuI GTATCC 2 cut(s) 232, 343
BglI GCCNNNNNGGC 1 cut(s) 403
BisI GCNGC 7 cut(s) 81, 84, 437, 1156, 1248, 1458, 2132
BlnI CCTAGG 1 cut(s) 731
BlpI GCTNAGC 1 cut(s) 1620
BlsI GCNGC 7 cut(s) 82, 85, 438, 1157, 1249, 1459, 2133
BmcAI AGTACT 1 cut(s) 1803
Bme1390I CCNGG 5 cut(s) 138, 171, 362, 441, 522
Bme18I GGWCC 2 cut(s) 231, 890
BmeT110I CYCGRG 3 cut(s) 59, 112, 536
BmgT120I GGNCC 5 cut(s) 231, 766, 864, 890, 1508
BmiI GGNNCC 3 cut(s) 513, 865, 892
BmrFI CCNGG 5 cut(s) 138, 171, 362, 441, 522
BmrI ACTGGG 1 cut(s) 257
BmsI GCATC 5 cut(s) 607, 1408, 1723, 1745, 2067
BmuI ACTGGG 1 cut(s) 257
BpiI GAAGAC 3 cut(s) 1674, 1771, 2106
BplI GAGNNNNNCTC 2 cut(s) 727, 759
BpmI CTGGAG 1 cut(s) 2076
Bpu10I CCTNAGC 1 cut(s) 677
Bpu1102I GCTNAGC 1 cut(s) 1620
BpuEI CTTGAG 2 cut(s) 388, 1779
BsaBI GATNNNNATC 2 cut(s) 1685, 1932
BsaI GGTCTC 1 cut(s) 828
BsaJI CCNNGG 3 cut(s) 136, 731, 1579
BsaWI WCCGGW 2 cut(s) 353, 1029
Bsc4I CCNNNNNNNGG 6 cut(s) 141, 240, 521, 807, 1436, 1557
Bse118I RCCGGY 1 cut(s) 1029
Bse1I ACTGG 2 cut(s) 263, 2199
Bse3DI GCAATG 6 cut(s) 812, 845, 886, 1055, 1788, 2146
Bse8I GATNNNNATC 2 cut(s) 1685, 1932
BseBI CCWGG 5 cut(s) 138, 171, 362, 441, 522
BseDI CCNNGG 3 cut(s) 136, 731, 1579
BseGI GGATG 6 cut(s) 284, 418, 622, 1166, 1970, 2058
BseJI GATNNNNATC 2 cut(s) 1685, 1932
BseLI CCNNNNNNNGG 6 cut(s) 141, 240, 521, 807, 1436, 1557
BseMI GCAATG 6 cut(s) 812, 845, 886, 1055, 1788, 2146
BseMII CTCAG 4 cut(s) 691, 1245, 1252, 1851
BseNI ACTGG 2 cut(s) 263, 2199
BseRI GAGGAG 3 cut(s) 310, 313, 316
BseXI GCAGC 7 cut(s) 70, 92, 423, 1167, 1259, 1444, 2118
BsgI GTGCAG 1 cut(s) 501
Bsh1285I CGRYCG 1 cut(s) 214
BshFI GGCC 4 cut(s) 768, 866, 1509, 1578
BshTI ACCGGT 1 cut(s) 1029
BsiEI CGRYCG 1 cut(s) 214
BsiHKAI GWGCWC 1 cut(s) 156
BsiHKCI CYCGRG 3 cut(s) 59, 112, 536
BsiSI CCGG 2 cut(s) 354, 1030
BslFI GGGAC 1 cut(s) 876
BslI CCNNNNNNNGG 6 cut(s) 141, 240, 521, 807, 1436, 1557
BsmAI GTCTC 3 cut(s) 136, 828, 1318
BsmFI GGGAC 1 cut(s) 876
BsmI GAATGC 1 cut(s) 1927
BsnI GGCC 4 cut(s) 768, 866, 1509, 1578
Bso31I GGTCTC 1 cut(s) 828
BsoBI CYCGRG 3 cut(s) 59, 112, 536
Bsp1286I GDGCHC 2 cut(s) 156, 657
Bsp143I GATC 8 cut(s) 90, 415, 511, 608, 727, 985, 1686, 2098
Bsp1720I GCTNAGC 1 cut(s) 1620
BspACI CCGC 5 cut(s) 330, 637, 1097, 1158, 1911
BspANI GGCC 4 cut(s) 768, 866, 1509, 1578
BspCNI CTCAG 4 cut(s) 690, 1246, 1251, 1852
BspHI TCATGA 1 cut(s) 1596
BspLI GGNNCC 3 cut(s) 513, 865, 892
BspMAI CTGCAG 1 cut(s) 2136
BspMI ACCTGC 1 cut(s) 2182
BspPI GGATC 4 cut(s) 506, 519, 616, 722
BspTNI GGTCTC 1 cut(s) 828
BsrDI GCAATG 6 cut(s) 812, 845, 886, 1055, 1788, 2146
BsrFI RCCGGY 1 cut(s) 1029
BsrI ACTGG 2 cut(s) 263, 2199
BssAI RCCGGY 1 cut(s) 1029
BssECI CCNNGG 3 cut(s) 136, 731, 1579
BssMI GATC 8 cut(s) 90, 415, 511, 608, 727, 985, 1686, 2098
BssT1I CCWWGG 2 cut(s) 731, 1579
Bst2UI CCWGG 5 cut(s) 138, 171, 362, 441, 522
Bst4CI ACNGT 4 cut(s) 466, 1110, 1820, 2248
Bst6I CTCTTC 4 cut(s) 494, 1987, 2160, 2218
BstC8I GCNNGC 2 cut(s) 1015, 2036
BstDEI CTNAG 6 cut(s) 677, 1118, 1238, 1254, 1620, 1860
BstENI CCTNNNNNAGG 1 cut(s) 519
BstF5I GGATG 6 cut(s) 284, 418, 622, 1166, 1970, 2058
BstKTI GATC 8 cut(s) 93, 418, 514, 611, 730, 988, 1689, 2101
BstMAI GTCTC 3 cut(s) 136, 828, 1318
BstMBI GATC 8 cut(s) 90, 415, 511, 608, 727, 985, 1686, 2098
BstMCI CGRYCG 1 cut(s) 214
BstMWI GCNNNNNNNGC 7 cut(s) 403, 433, 1010, 1122, 1131, 1454, 1967
BstNI CCWGG 5 cut(s) 138, 171, 362, 441, 522
BstNSI RCATGY 6 cut(s) 202, 342, 1054, 1702, 1828, 2034
BstSCI CCNGG 5 cut(s) 136, 169, 360, 439, 520
BstSFI CTRYAG 1 cut(s) 2132
BstV1I GCAGC 7 cut(s) 70, 92, 423, 1167, 1259, 1444, 2118
BstV2I GAAGAC 3 cut(s) 1674, 1771, 2106
BstX2I RGATCY 1 cut(s) 511
BstYI RGATCY 1 cut(s) 511
BsuI GTATCC 2 cut(s) 232, 343
BsuRI GGCC 4 cut(s) 768, 866, 1509, 1578
BtsCI GGATG 6 cut(s) 284, 418, 622, 1166, 1970, 2058
BtsI GCAGTG 3 cut(s) 333, 565, 1134
BtsIMutI CAGTG 4 cut(s) 333, 462, 565, 1134
BveI ACCTGC 1 cut(s) 2182
Cac8I GCNNGC 2 cut(s) 1015, 2036
CaiI CAGNNNCTG 1 cut(s) 1253
CciI TCATGA 1 cut(s) 1596
Cfr10I RCCGGY 1 cut(s) 1029
Cfr13I GGNCC 5 cut(s) 231, 766, 864, 890, 1508
CsiI ACCWGGT 3 cut(s) 169, 360, 520
Csp6I GTAC 5 cut(s) 64, 205, 648, 1696, 1802
CspAI ACCGGT 1 cut(s) 1029
CviQI GTAC 5 cut(s) 64, 205, 648, 1696, 1802
DdeI CTNAG 6 cut(s) 677, 1118, 1238, 1254, 1620, 1860
DpnI GATC 8 cut(s) 92, 417, 513, 610, 729, 987, 1688, 2100
DpnII GATC 8 cut(s) 90, 415, 511, 608, 727, 985, 1686, 2098
DraI TTTAAA 1 cut(s) 951
Eam1104I CTCTTC 4 cut(s) 494, 1987, 2160, 2218
EarI CTCTTC 4 cut(s) 494, 1987, 2160, 2218
EciI GGCGGA 1 cut(s) 319
Ecl136II GAGCTC 1 cut(s) 154
Eco130I CCWWGG 2 cut(s) 731, 1579
Eco24I GRGCYC 2 cut(s) 156, 657
Eco31I GGTCTC 1 cut(s) 828
Eco47I GGWCC 2 cut(s) 231, 890
Eco53kI GAGCTC 1 cut(s) 154
Eco88I CYCGRG 3 cut(s) 59, 112, 536
EcoICRI GAGCTC 1 cut(s) 154
EcoNI CCTNNNNNAGG 1 cut(s) 519
EcoO109I RGGNCCY 2 cut(s) 864, 890
EcoRI GAATTC 1 cut(s) 796
EcoRII CCWGG 5 cut(s) 136, 169, 360, 439, 520
EcoT14I CCWWGG 2 cut(s) 731, 1579
EcoT22I ATGCAT 1 cut(s) 1738
EcoT38I GRGCYC 2 cut(s) 156, 657
ErhI CCWWGG 2 cut(s) 731, 1579
FalI AAGNNNNNCTT 4 cut(s) 1114, 1146, 1788, 1820
FaqI GGGAC 1 cut(s) 876
FbaI TGATCA 2 cut(s) 415, 2098
FblI GTMKAC 1 cut(s) 1515
Fnu4HI GCNGC 7 cut(s) 81, 84, 437, 1156, 1248, 1458, 2132
FokI GGATG 6 cut(s) 271, 405, 629, 1173, 1977, 2045
FriOI GRGCYC 2 cut(s) 156, 657
Fsp4HI GCNGC 7 cut(s) 81, 84, 437, 1156, 1248, 1458, 2132
FspBI CTAG 7 cut(s) 77, 381, 588, 603, 732, 1047, 1754
GluI GCNGC 7 cut(s) 81, 84, 437, 1156, 1248, 1458, 2132
GsuI CTGGAG 1 cut(s) 2076
HaeIII GGCC 4 cut(s) 768, 866, 1509, 1578
HapII CCGG 2 cut(s) 354, 1030
HincII GTYRAC 1 cut(s) 306
HindIII AAGCTT 1 cut(s) 2046
HinfI GANTC 6 cut(s) 377, 747, 772, 1954, 2015, 2144
HpaII CCGG 2 cut(s) 354, 1030
HphI GGTGA 6 cut(s) 703, 943, 1418, 1544, 2017, 2242
Hpy166II GTNNAC 7 cut(s) 73, 176, 207, 306, 1106, 1516, 2209
Hpy188III TCNNGA 9 cut(s) 59, 367, 744, 975, 1037, 1541, 1597, 1664, 2084
Hpy8I GTNNAC 7 cut(s) 73, 176, 207, 306, 1106, 1516, 2209
HpyAV CCTTC 6 cut(s) 695, 877, 881, 922, 1996, 2261
HpyCH4III ACNGT 4 cut(s) 466, 1110, 1820, 2248
HpyCH4IV ACGT 1 cut(s) 1768
HpyF10VI GCNNNNNNNGC 7 cut(s) 403, 433, 1010, 1122, 1131, 1454, 1967
HpyF3I CTNAG 6 cut(s) 677, 1118, 1238, 1254, 1620, 1860
HpySE526I ACGT 1 cut(s) 1768
Ksp22I TGATCA 2 cut(s) 415, 2098
Kzo9I GATC 8 cut(s) 90, 415, 511, 608, 727, 985, 1686, 2098
LmnI GCTCC 2 cut(s) 1629, 2095
Lsp1109I GCAGC 7 cut(s) 70, 92, 423, 1167, 1259, 1444, 2118
LweI GCATC 5 cut(s) 607, 1408, 1723, 1745, 2067
MabI ACCWGGT 3 cut(s) 169, 360, 520
MaeI CTAG 7 cut(s) 77, 381, 588, 603, 732, 1047, 1754
MaeII ACGT 1 cut(s) 1768
MaeIII GTNAC 7 cut(s) 466, 685, 1211, 1274, 1820, 1827, 2248
MalI GATC 8 cut(s) 92, 417, 513, 610, 729, 987, 1688, 2100
MboI GATC 8 cut(s) 90, 415, 511, 608, 727, 985, 1686, 2098
MfeI CAATTG 1 cut(s) 1308
MflI RGATCY 1 cut(s) 511
MhlI GDGCHC 2 cut(s) 156, 657
MlyI GAGTC 2 cut(s) 386, 1948
MmeI TCCRAC 2 cut(s) 446, 1651
Mph1103I ATGCAT 1 cut(s) 1738
MroXI GAANNNNTTC 1 cut(s) 1126
MseI TTAA 6 cut(s) 314, 459, 626, 950, 1442, 1607
MslI CAYNNNNRTG 1 cut(s) 197
MspA1I CMGCKG 4 cut(s) 83, 781, 1158, 2096
MspI CCGG 2 cut(s) 354, 1030
MspR9I CCNGG 5 cut(s) 138, 171, 362, 441, 522
MunI CAATTG 1 cut(s) 1308
Mva1269I GAATGC 1 cut(s) 1927
MvaI CCWGG 5 cut(s) 138, 171, 362, 441, 522
MwoI GCNNNNNNNGC 7 cut(s) 403, 433, 1010, 1122, 1131, 1454, 1967
NdeII GATC 8 cut(s) 90, 415, 511, 608, 727, 985, 1686, 2098
NlaIV GGNNCC 3 cut(s) 513, 865, 892
NmeAIII GCCGAG 1 cut(s) 400
NmuCI GTSAC 2 cut(s) 1211, 2248
NsiI ATGCAT 1 cut(s) 1738
NspI RCATGY 6 cut(s) 202, 342, 1054, 1702, 1828, 2034
PaeR7I CTCGAG 2 cut(s) 59, 536
PagI TCATGA 1 cut(s) 1596
PciI ACATGT 1 cut(s) 1824
PcsI WCGNNNNNNNCGW 1 cut(s) 33
PctI GAATGC 1 cut(s) 1927
PdmI GAANNNNTTC 1 cut(s) 1126
PfeI GAWTC 4 cut(s) 747, 772, 2015, 2144
PflMI CCANNNNNTGG 2 cut(s) 240, 807
PinAI ACCGGT 1 cut(s) 1029
PkrI GCNGC 7 cut(s) 82, 85, 438, 1157, 1249, 1459, 2133
PleI GAGTC 2 cut(s) 385, 1948
PpsI GAGTC 2 cut(s) 385, 1948
PpuMI RGGWCCY 1 cut(s) 890
PscI ACATGT 1 cut(s) 1824
Psp124BI GAGCTC 1 cut(s) 156
Psp5II RGGWCCY 1 cut(s) 890
Psp6I CCWGG 5 cut(s) 136, 169, 360, 439, 520
PspGI CCWGG 5 cut(s) 136, 169, 360, 439, 520
PspN4I GGNNCC 3 cut(s) 513, 865, 892
PspPI GGNCC 5 cut(s) 231, 766, 864, 890, 1508
PspPPI RGGWCCY 1 cut(s) 890
PspXI VCTCGAGB 1 cut(s) 536
PstI CTGCAG 1 cut(s) 2136
PstNI CAGNNNCTG 1 cut(s) 1253
PsuI RGATCY 1 cut(s) 511
PvuII CAGCTG 3 cut(s) 83, 781, 2096
RsaI GTAC 5 cut(s) 65, 206, 649, 1697, 1803
RsaNI GTAC 5 cut(s) 64, 205, 648, 1696, 1802
RseI CAYNNNNRTG 1 cut(s) 197
SacI GAGCTC 1 cut(s) 156
SaqAI TTAA 6 cut(s) 314, 459, 626, 950, 1442, 1607
SatI GCNGC 7 cut(s) 81, 84, 437, 1156, 1248, 1458, 2132
Sau3AI GATC 8 cut(s) 90, 415, 511, 608, 727, 985, 1686, 2098
Sau96I GGNCC 5 cut(s) 231, 766, 864, 890, 1508
ScaI AGTACT 1 cut(s) 1803
SchI GAGTC 2 cut(s) 386, 1948
ScrFI CCNGG 5 cut(s) 138, 171, 362, 441, 522
SduI GDGCHC 2 cut(s) 156, 657
SexAI ACCWGGT 3 cut(s) 169, 360, 520
SfaNI GCATC 5 cut(s) 607, 1408, 1723, 1745, 2067
SfcI CTRYAG 1 cut(s) 2132
Sfr274I CTCGAG 2 cut(s) 59, 536
SinI GGWCC 2 cut(s) 231, 890
SlaI CTCGAG 2 cut(s) 59, 536
SmiMI CAYNNNNRTG 1 cut(s) 197
SmlI CTYRAG 4 cut(s) 59, 367, 536, 1794
SmoI CTYRAG 4 cut(s) 59, 367, 536, 1794
SpeI ACTAGT 1 cut(s) 602
SsiI CCGC 5 cut(s) 330, 637, 1097, 1158, 1911
SspMI CTAG 7 cut(s) 77, 381, 588, 603, 732, 1047, 1754
SstI GAGCTC 1 cut(s) 156
StyD4I CCNGG 5 cut(s) 136, 169, 360, 439, 520
StyI CCWWGG 2 cut(s) 731, 1579
TaaI ACNGT 4 cut(s) 466, 1110, 1820, 2248
TaiI ACGT 1 cut(s) 1771
TaqI TCGA 7 cut(s) 60, 537, 745, 974, 1166, 1952, 2272
TaqII GACCGA 1 cut(s) 228
TatI WGTACW 2 cut(s) 1695, 1801
TfiI GAWTC 4 cut(s) 747, 772, 2015, 2144
Tru1I TTAA 6 cut(s) 314, 459, 626, 950, 1442, 1607
Tru9I TTAA 6 cut(s) 314, 459, 626, 950, 1442, 1607
TscAI CASTG 4 cut(s) 340, 469, 565, 1141
TseFI GTSAC 2 cut(s) 1211, 2248
TseI GCWGC 7 cut(s) 80, 83, 436, 1155, 1247, 1457, 2131
Tsp45I GTSAC 2 cut(s) 1211, 2248
TspGWI ACGGA 5 cut(s) 25, 567, 1191, 1375, 2004
TspRI CASTG 4 cut(s) 340, 469, 565, 1141
Van91I CCANNNNNTGG 2 cut(s) 240, 807
VpaK11BI GGWCC 2 cut(s) 231, 890
XagI CCTNNNNNAGG 1 cut(s) 519
XapI RAATTY 6 cut(s) 310, 483, 796, 811, 1524, 2194
XceI RCATGY 6 cut(s) 202, 342, 1054, 1702, 1828, 2034
XcmI CCANNNNNNNNNTGG 3 cut(s) 241, 429, 1711
XhoI CTCGAG 2 cut(s) 59, 536
XmaJI CCTAGG 1 cut(s) 731
XmiI GTMKAC 1 cut(s) 1515
XmnI GAANNNNTTC 1 cut(s) 1126
XspI CTAG 7 cut(s) 77, 381, 588, 603, 732, 1047, 1754
ZrmI AGTACT 1 cut(s) 1803
Zsp2I ATGCAT 1 cut(s) 1738
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.