Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr4

Orthogroup ID

OG0000564

Physical Location & Seq

Genomic Coordinates Forward (+)

25324629 .. 25328171

Gene Structure 3543 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw4G010830.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2472 bp

ATGGCATGCTGCTTGCCTGCAAATTTTTTGTGCAGCCAATCTACTGACTTGATGTTTTCCATTCTTTTGTTATGCCTCTGTCTCAAGACTCATGATATCTCCTTTGCAGCTGACACCATCACTGTAAACCAATCTCTCTCTGGTGATCAGACCATTGTCTCGGAACATGGGGTTTTTGAACTGGGTTTCTTCAAACCAGGTAATAATTCAAATTACTATATAGGCATCTGGTATTACAAGCAAGTTGTGTCTGTGCAAACCATAGTCTGGGTGGCAAATAGGGAACGGTCAATCTCTGATAGATTTTCTTCAGTATTGACAATGTCAGATGGAAATTTAGTACTGTTTAATGAGTCCAAAAGCCCAGTTTGGTCTACAAATCTGTCCTCCACCACCTTAGGCTCTGTGAAAGCAGTTCTCTTAGATGATGGAAACCTTGTCTTAAGAGCTGGGTCCAATTCACCCCAACCTTTATGGCAAAGCTTTGATCATCCAGCCCATACTTGGCTACCAGGAAGTAAACTAGGGTTCAACAATGTTACCAAACAAACCCAAATGCTCACTTCATGGAAGAATGCGGAGAACCCTGCACCGGGTCCGTTCTCTCTTCAGCTAGACCCAAATGGAAGCAACTCACTTATCATATGGTTGAATTACCTGAATATGTCTGAACAGTATTGGAGCACTGGACCTTGGGATGATAGTCTTCATACTTTCTGCTTGAGTCCTGAAATGAGCCTCAATAATATCTTCAATTACAGCCATGTTACCAACGAAAATGGAAGCTATTTCACCTATTCTGTTTATAACCCCAGTGAAGTATCTCGATTAGTGATGTACTTCTCGGGGCAGGTTTGGCAACTAACATGGAAGACCAATAAATGGAACTTGGTTTGGTCTAAACCAAATGTTTATGCCTTTTGTGGGCCATTCTCCACTTACAGTCAGGAATTCTTGCGTTTCTGTAATTGTTTGAATGGTTTTGGGCCAAAATCGCAGAGTGAGTGGGATTTGCAGGATTATTCTGGTGGGTGTTCGAGAAATACTACTTTGAATTGTGGGAACGGGACGAAAGAAGACCAGTTCATACAAATACCTAGCAAGCCATTGTCTGCATCTCAACAGCTTCAGCTAGGAAGTATTGAGGATTGTGAATCAATCTGTTTAAGAAAGTGCGATTGCTCTGCTTATGGTTATGGAATTACTACTGGATGTGAAATCTGGACAGGAGATCTCTTGGCAGATGACAAAGGAAGAACTCTACACATCAGAGTACCGGCAGCTGTATCAAAGAATCCTAGTTTTAAGCGGTCCCTAGTGATTGGACTAGTCTCAGCAATAACAGGATTGCTTACAATAATTTGTAGCTATTTCTTGTGGAAGAAAACTACAAGGAAGAGAAAGAAGCGTCAGAGGAAAGATGGTGAGATCATAAGTAATGTTGGTGGAAAGAATGACACAGAACTACCAGTGTTTGACTTGAAGAGTATATTAGCTGCTACTGAAGACTTTTCTGCAGCAAATAAACTGGGTGAGGGAGGATTTGGCCCTGTTTATAAGGGTATTTTGCCTGGAAATCGAAAGGTGGCCATAAAAATGCTGTCAAAGAAGTCTGGTCAAGGGCATCTAGAGTTCATGAATGAGTTGAAGCTGATAGCCAAGCTCCAACATACCAATCTTGTTAGGCTTTTGGGTTGTTGTATTGAAGAGGAGGAAAGGATGTTGATCTATGAGTACATGCCTAATAGAAGTTTGGACAAATTTTTGTTTGATCCATCTGAAAGAACAAAATTGGATTGGGATAGACGCTTTAGAATTATAGAAGGCATTGGTCAAGGACTACTTTATATCCACAAGTACTCGAGATTGAAAATCATTCATAGGGACCTAAAAGCGAGTAATATTTTGTTGGATGAAGCAATGAATCCTAAAATCTCAGACTTTGGAATGGCAAGGATATTCGACATGAATCAAACTGAAGCAAATACCAATAGGGTTGTTGGGACATACGGTTACATGTCACCTGAATATGCACGCTATGGCCATTTCTCTGAGAAATCGGATGTATTTAGTTTTGGAGTACTGCTGTTGGAGATTATAAGTGGAAAGAAGAATGCTGCTCTCTATCGTTATGAAAATTCACTTACTCTTGCTGGATGGGCGTGGAAACTGTGGAAAAAGGGTAGGGGAATGGAGGTAATTGATGAATCTGTAAGGGAAACATGTCGACCTCATGAAGCTTTAAGGTGTATCCATGTAGGGCTTCTGTGTGTTCAAGAAGCCCCAGCTGATCGACCAACAATGTTTTCTGTAGTTCATATGTTGCAAGCCGACGAAAGTACAACACTTCCTCCCTCCAAAGAACCTGCATTTTCAACACACAAGAATTCCAGTGCCGCTAGTTCTTCTTCTCAAACATCTAGAAATTTTTCCGCCAATCAATTGACCGTTAGTATCATAGAAGGTCGATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

823

Amino Acids

92.44

Weight (kDa)

8.3

Isoelectric Point (pI)

43.05

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
B_lectin	PF01453	87 - 191	2.3e-33	D-mannose binding lectin
S_locus_glycop	PF00954	227 - 332	6.9e-16	S-locus glycoprotein domain
PAN_2	PF08276	356 - 413	1.9e-09	PAN-like domain
Pkinase	PF00069	506 - 707	1.8e-46	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	507 - 774	1.5e-51	Protein tyrosine and serine/threonine kinase
DUF3403	PF11883	779 - 823	4.1e-10	Domain of unknown function (DUF3403)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000564)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g44064 FvH4_6g44064 FvH4_6g44100 FvH4_6g44101 FvH4_6g51830
malus_domestica	MD09G1094400.v1.1 MD09G1094800.v1.1 MD09G1095200.v1.1 MD09G1095900.v1.1 MD09G1096000.v1.1 MD09G1096200.v1.1 MD17G1083900.v1.1 MD17G1088500.v1.1
prunus_persica	Prupe.3G224900_v2.0.a1 Prupe.3G224900_v2.0.a1 Prupe.3G224900_v2.0.a1 Prupe.3G224900_v2.0.a1 Prupe.3G224900_v2.0.a1 Prupe.3G224900_v2.0.a1 Prupe.3G225100_v2.0.a1 Prupe.3G225100_v2.0.a1 Prupe.3G225100_v2.0.a1 Prupe.3G225200_v2.0.a1 Prupe.3G225200_v2.0.a1
pyrus_communis	pycom09g01920 pycom09g01930 pycom09g01940 pycom09g01950 pycom09g02000 pycom09g02050 pycom09g02070 pycom17g08540
rosa_chinensis	RchiOBHm_Chr1g0318601 RchiOBHm_Chr1g0318761 RchiOBHm_Chr1g0342541 RchiOBHm_Chr2g0160651 RchiOBHm_Chr2g0160831 RchiOBHm_Chr4g0405661 RchiOBHm_Chr4g0405691 RchiOBHm_Chr4g0405701 RchiOBHm_Chr7g0238591
rosa_laevigata	RLG00000002292 RLG00000008826 RLG00000008828 RLG00000008829 RLG00000021243 RLG00000021245 RLG00000030522
rosa_multiflora	Rmu_co8432573.1_g000001 Rmu_sc0001200.1_g000041 Rmu_sc0001200.1_g000043 Rmu_sc0002545.1_g000017 Rmu_sc0002773.1_g000017 Rmu_sc0012550.1_g000008
rosa_roxburghii	Rroxscaffold_2G00089500 Rroxscaffold_5G00350130 Rroxscaffold_5G00350180
rosa_rugosa	Rorug01G0022300 Rorug04G0061600
rosa_samantha	Rh1AG035300 Rh1CG034300 Rh1DG036100 Rh1DG149100 Rh2AG550900 Rh2BG564200 Rh2BG565500 Rh2CG535300 Rh2DG574400 Rh4AG133100 Rh4AG133200 Rh4AG133300 Rh4BG128200 Rh4CG141000 Rh4DG127700 Rh4DG127900
rosa_wichuraiana	Rw2G045540 Rw2G045650 Rw4G010790 Rw4G010800 Rw4G010810 Rw4G010830 Rw7G027620 Rw7G027630

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	807, 1557, 2099
Acc36I	ACCTGC	2 cut(s)	841, 2374
AccB7I	CCANNNNNTGG	2 cut(s)	267, 882
AccI	GTMKAC	2 cut(s)	374, 2227
AciI	CCGC	4 cut(s)	578, 1309, 2397, 2433
AclWI	GGATC	1 cut(s)	1766
AcoI	YGGCCR	2 cut(s)	1587, 2041
AcsI	RAATTY	7 cut(s)	22, 334, 950, 1760, 2137, 2386, 2425
AcuI	CTGAAG	5 cut(s)	294, 593, 1112, 1524, 1998
AfaI	GTAC	7 cut(s)	342, 839, 1275, 1736, 1859, 2082, 2341
AfiI	CCNNNNNNNGG	6 cut(s)	267, 504, 592, 593, 882, 924
AflII	CTTAAG	1 cut(s)	442
AflIII	ACRYGT	2 cut(s)	2016, 2222
AhlI	ACTAGT	1 cut(s)	1327
AjnI	CCWGG	3 cut(s)	196, 511, 1570
AjuI	GAANNNNNNNTTGG	2 cut(s)	2290, 2322
Alw21I	GWGCWC	1 cut(s)	686
Alw26I	GTCTC	3 cut(s)	86, 163, 1336
AlwI	GGATC	1 cut(s)	1766
Ama87I	CYCGRG	2 cut(s)	844, 1861
AoxI	GGCC	5 cut(s)	926, 986, 1546, 1587, 2041
ApeKI	GCWGC	7 cut(s)	9, 33, 107, 1280, 1496, 1517, 2117
ApoI	RAATTY	7 cut(s)	22, 334, 950, 1760, 2137, 2386, 2425
Asp700I	GAANNNNTTC	1 cut(s)	2428
AspS9I	GGNCC	8 cut(s)	453, 596, 689, 926, 986, 1311, 1547, 1885
AsuC2I	CCSGG	1 cut(s)	594
AsuHPI	GGTGA	6 cut(s)	155, 453, 784, 1436, 1544, 2013
AvaI	CYCGRG	2 cut(s)	844, 1861
AvaII	GGWCC	5 cut(s)	453, 596, 689, 1311, 1885
AxyI	CCTNAGG	1 cut(s)	397
BalI	TGGCCA	2 cut(s)	1589, 2043
BbsI	GAAGAC	4 cut(s)	698, 878, 1083, 1512
Bbv12I	GWGCWC	1 cut(s)	686
BbvI	GCAGC	6 cut(s)	45, 119, 1292, 1483, 1529, 2104
BccI	CCATC	6 cut(s)	125, 323, 422, 1415, 1783, 2151
BcgI	CGANNNNNNTGC	2 cut(s)	1166, 1200
BciT130I	CCWGG	3 cut(s)	198, 513, 1572
BciVI	GTATCC	1 cut(s)	2261
BclI	TGATCA	2 cut(s)	145, 487
BcnI	CCSGG	1 cut(s)	594
BcoDI	GTCTC	3 cut(s)	86, 163, 1336
BcuI	ACTAGT	1 cut(s)	1327
BfmI	CTRYAG	2 cut(s)	1515, 2310
BfrI	CTTAAG	1 cut(s)	442
BfuAI	ACCTGC	2 cut(s)	841, 2374
BfuI	GTATCC	1 cut(s)	2261
BglII	AGATCT	1 cut(s)	1231
BisI	GCNGC	8 cut(s)	10, 34, 108, 1281, 1497, 1518, 2118, 2397
BlsI	GCNGC	8 cut(s)	11, 35, 109, 1282, 1498, 1519, 2119, 2398
BmcAI	AGTACT	3 cut(s)	342, 1859, 2082
Bme1390I	CCNGG	4 cut(s)	198, 513, 594, 1572
Bme18I	GGWCC	5 cut(s)	453, 596, 689, 1311, 1885
BmeT110I	CYCGRG	2 cut(s)	844, 1861
BmgT120I	GGNCC	8 cut(s)	453, 596, 689, 926, 986, 1311, 1547, 1885
BmiI	GGNNCC	4 cut(s)	454, 597, 1313, 1886
BmrFI	CCNGG	4 cut(s)	198, 513, 594, 1572
BmrI	ACTGGG	4 cut(s)	191, 359, 807, 1538
BmsI	GCATC	3 cut(s)	234, 1124, 1633
BmuI	ACTGGG	4 cut(s)	191, 359, 807, 1538
BoxI	GACNNNNGTC	1 cut(s)	155
BpiI	GAAGAC	4 cut(s)	698, 878, 1083, 1512
BpuEI	CTTGAG	2 cut(s)	68, 742
BpuMI	CCSGG	1 cut(s)	594
BsaBI	GATNNNNATC	1 cut(s)	1724
BsaJI	CCNNGG	1 cut(s)	692
BsaXI	ACNNNNNCTCC	2 cut(s)	2335, 2365
Bsc4I	CCNNNNNNNGG	6 cut(s)	267, 504, 592, 593, 882, 924
Bse118I	RCCGGY	1 cut(s)	1276
Bse1I	ACTGG	9 cut(s)	186, 365, 691, 813, 1081, 1213, 1469, 1533, 2391
Bse21I	CCTNAGG	1 cut(s)	397
Bse3DI	GCAATG	1 cut(s)	1926
Bse8I	GATNNNNATC	1 cut(s)	1724
BseBI	CCWGG	3 cut(s)	198, 513, 1572
BseDI	CCNNGG	1 cut(s)	692
BseGI	GGATG	7 cut(s)	490, 703, 1217, 1725, 1918, 2068, 2162
BseJI	GATNNNNATC	1 cut(s)	1724
BseLI	CCNNNNNNNGG	6 cut(s)	267, 504, 592, 593, 882, 924
BseMI	GCAATG	1 cut(s)	1926
BseMII	CTCAG	3 cut(s)	1347, 1950, 2043
BseNI	ACTGG	9 cut(s)	186, 365, 691, 813, 1081, 1213, 1469, 1533, 2391
BseRI	GAGGAG	1 cut(s)	1724
BseXI	GCAGC	6 cut(s)	45, 119, 1292, 1483, 1529, 2104
BseYI	CCCAGC	2 cut(s)	449, 2284
BsgI	GTGCAG	2 cut(s)	52, 573
BshFI	GGCC	5 cut(s)	928, 988, 1548, 1589, 2043
BsiHKAI	GWGCWC	1 cut(s)	686
BsiHKCI	CYCGRG	2 cut(s)	844, 1861
BsiSI	CCGG	2 cut(s)	593, 1277
BslFI	GGGAC	4 cut(s)	1081, 1297, 1898, 2017
BslI	CCNNNNNNNGG	6 cut(s)	267, 504, 592, 593, 882, 924
BsmAI	GTCTC	3 cut(s)	86, 163, 1336
BsmFI	GGGAC	4 cut(s)	1081, 1297, 1898, 2017
BsmI	GAATGC	2 cut(s)	580, 2119
BsnI	GGCC	5 cut(s)	928, 988, 1548, 1589, 2043
BsoBI	CYCGRG	2 cut(s)	844, 1861
Bsp1286I	GDGCHC	1 cut(s)	686
Bsp143I	GATC	7 cut(s)	145, 487, 1231, 1428, 1725, 1771, 2290
BspACI	CCGC	4 cut(s)	578, 1309, 2397, 2433
BspANI	GGCC	5 cut(s)	928, 988, 1548, 1589, 2043
BspCNI	CTCAG	3 cut(s)	1346, 1949, 2044
BspHI	TCATGA	3 cut(s)	91, 1635, 2233
BspLI	GGNNCC	4 cut(s)	454, 597, 1313, 1886
BspMAI	CTGCAG	1 cut(s)	1519
BspMI	ACCTGC	2 cut(s)	841, 2374
BspPI	GGATC	1 cut(s)	1766
BspTI	CTTAAG	1 cut(s)	442
BsrDI	GCAATG	1 cut(s)	1926
BsrFI	RCCGGY	1 cut(s)	1276
BsrI	ACTGG	9 cut(s)	186, 365, 691, 813, 1081, 1213, 1469, 1533, 2391
BssAI	RCCGGY	1 cut(s)	1276
BssECI	CCNNGG	1 cut(s)	692
BssMI	GATC	7 cut(s)	145, 487, 1231, 1428, 1725, 1771, 2290
BssT1I	CCWWGG	1 cut(s)	692
Bst2UI	CCWGG	3 cut(s)	198, 513, 1572
Bst4CI	ACNGT	8 cut(s)	124, 288, 345, 675, 944, 2012, 2172, 2449
Bst6I	CTCTTC	4 cut(s)	612, 1391, 1478, 1701
BstAFI	CTTAAG	1 cut(s)	442
BstC8I	GCNNGC	6 cut(s)	7, 14, 18, 1103, 2035, 2328
BstDEI	CTNAG	5 cut(s)	397, 421, 1333, 1936, 2052
BstF5I	GGATG	7 cut(s)	490, 703, 1217, 1725, 1918, 2068, 2162
BstKTI	GATC	7 cut(s)	148, 490, 1234, 1431, 1728, 1774, 2293
BstMAI	GTCTC	3 cut(s)	86, 163, 1336
BstMBI	GATC	7 cut(s)	145, 487, 1231, 1428, 1725, 1771, 2290
BstMWI	GCNNNNNNNGC	3 cut(s)	856, 994, 2159
BstNI	CCWGG	3 cut(s)	198, 513, 1572
BstNSI	RCATGY	4 cut(s)	9, 1741, 2020, 2226
BstPAI	GACNNNNGTC	1 cut(s)	155
BstSCI	CCNGG	4 cut(s)	196, 511, 592, 1570
BstSFI	CTRYAG	2 cut(s)	1515, 2310
BstV1I	GCAGC	6 cut(s)	45, 119, 1292, 1483, 1529, 2104
BstV2I	GAAGAC	4 cut(s)	698, 878, 1083, 1512
BstX2I	RGATCY	1 cut(s)	1231
BstYI	RGATCY	1 cut(s)	1231
Bsu36I	CCTNAGG	1 cut(s)	397
BsuI	GTATCC	1 cut(s)	2261
BsuRI	GGCC	5 cut(s)	928, 988, 1548, 1589, 2043
BtsCI	GGATG	7 cut(s)	490, 703, 1217, 1725, 1918, 2068, 2162
BtsIMutI	CAGTG	5 cut(s)	120, 684, 820, 1476, 2398
BveI	ACCTGC	2 cut(s)	841, 2374
Cac8I	GCNNGC	6 cut(s)	7, 14, 18, 1103, 2035, 2328
CciI	TCATGA	3 cut(s)	91, 1635, 2233
Cfr10I	RCCGGY	1 cut(s)	1276
Cfr13I	GGNCC	8 cut(s)	453, 596, 689, 926, 986, 1311, 1547, 1885
CseI	GACGC	2 cut(s)	1397, 1815
CsiI	ACCWGGT	1 cut(s)	196
Csp6I	GTAC	7 cut(s)	341, 838, 1274, 1735, 1858, 2081, 2340
CviQI	GTAC	7 cut(s)	341, 838, 1274, 1735, 1858, 2081, 2340
DdeI	CTNAG	5 cut(s)	397, 421, 1333, 1936, 2052
DpnI	GATC	7 cut(s)	147, 489, 1233, 1430, 1727, 1773, 2292
DpnII	GATC	7 cut(s)	145, 487, 1231, 1428, 1725, 1771, 2290
EaeI	YGGCCR	2 cut(s)	1587, 2041
Eam1104I	CTCTTC	4 cut(s)	612, 1391, 1478, 1701
EarI	CTCTTC	4 cut(s)	612, 1391, 1478, 1701
EciI	GGCGGA	1 cut(s)	2422
Eco130I	CCWWGG	1 cut(s)	692
Eco32I	GATATC	1 cut(s)	97
Eco47I	GGWCC	5 cut(s)	453, 596, 689, 1311, 1885
Eco57I	CTGAAG	5 cut(s)	294, 593, 1112, 1524, 1998
Eco81I	CCTNAGG	1 cut(s)	397
Eco88I	CYCGRG	2 cut(s)	844, 1861
EcoO109I	RGGNCCY	1 cut(s)	1885
EcoRI	GAATTC	2 cut(s)	950, 2386
EcoRII	CCWGG	3 cut(s)	196, 511, 1570
EcoRV	GATATC	1 cut(s)	97
EcoT14I	CCWWGG	1 cut(s)	692
ErhI	CCWWGG	1 cut(s)	692
FalI	AAGNNNNNCTT	2 cut(s)	1827, 1859
FaqI	GGGAC	4 cut(s)	1081, 1297, 1898, 2017
FauNDI	CATATG	2 cut(s)	644, 2319
FbaI	TGATCA	2 cut(s)	145, 487
FblI	GTMKAC	2 cut(s)	374, 2227
Fnu4HI	GCNGC	8 cut(s)	10, 34, 108, 1281, 1497, 1518, 2118, 2397
FokI	GGATG	7 cut(s)	477, 710, 1224, 1732, 1925, 2075, 2169
Fsp4HI	GCNGC	8 cut(s)	10, 34, 108, 1281, 1497, 1518, 2118, 2397
GluI	GCNGC	8 cut(s)	10, 34, 108, 1281, 1497, 1518, 2118, 2397
GsaI	CCCAGC	2 cut(s)	453, 2288
HaeIII	GGCC	5 cut(s)	928, 988, 1548, 1589, 2043
HapII	CCGG	2 cut(s)	593, 1277
HgaI	GACGC	2 cut(s)	1397, 1815
HincII	GTYRAC	1 cut(s)	2228
HindII	GTYRAC	1 cut(s)	2228
HindIII	AAGCTT	2 cut(s)	481, 2238
HinfI	GANTC	8 cut(s)	88, 353, 724, 1154, 1294, 1924, 1969, 2207
HpaII	CCGG	2 cut(s)	593, 1277
HphI	GGTGA	6 cut(s)	155, 453, 784, 1436, 1544, 2013
Hpy166II	GTNNAC	4 cut(s)	127, 375, 521, 2228
Hpy8I	GTNNAC	4 cut(s)	127, 375, 521, 2228
Hpy99I	CGWCG	1 cut(s)	2336
HpyAV	CCTTC	2 cut(s)	1817, 2456
HpyCH4III	ACNGT	8 cut(s)	124, 288, 345, 675, 944, 2012, 2172, 2449
HpyF10VI	GCNNNNNNNGC	3 cut(s)	856, 994, 2159
HpyF3I	CTNAG	5 cut(s)	397, 421, 1333, 1936, 2052
Ksp22I	TGATCA	2 cut(s)	145, 487
Kzo9I	GATC	7 cut(s)	145, 487, 1231, 1428, 1725, 1771, 2290
LmnI	GCTCC	2 cut(s)	681, 1668
Lsp1109I	GCAGC	6 cut(s)	45, 119, 1292, 1483, 1529, 2104
LweI	GCATC	3 cut(s)	234, 1124, 1633
MabI	ACCWGGT	1 cut(s)	196
MaeIII	GTNAC	4 cut(s)	538, 766, 2012, 2019
MalI	GATC	7 cut(s)	147, 489, 1233, 1430, 1727, 1773, 2292
MboI	GATC	7 cut(s)	145, 487, 1231, 1428, 1725, 1771, 2290
MfeI	CAATTG	1 cut(s)	2441
MflI	RGATCY	1 cut(s)	1231
MhlI	GDGCHC	1 cut(s)	686
MlsI	TGGCCA	2 cut(s)	1589, 2043
MluNI	TGGCCA	2 cut(s)	1589, 2043
MlyI	GAGTC	3 cut(s)	82, 362, 733
MmeI	TCCRAC	3 cut(s)	1690, 1890, 2070
Mox20I	TGGCCA	2 cut(s)	1589, 2043
MroXI	GAANNNNTTC	1 cut(s)	2428
MscI	TGGCCA	2 cut(s)	1589, 2043
MseI	TTAA	5 cut(s)	348, 443, 1166, 1305, 2243
MslI	CAYNNNNRTG	1 cut(s)	1595
Msp20I	TGGCCA	2 cut(s)	1589, 2043
MspA1I	CMGCKG	3 cut(s)	110, 1283, 2288
MspCI	CTTAAG	1 cut(s)	442
MspI	CCGG	2 cut(s)	593, 1277
MspR9I	CCNGG	4 cut(s)	198, 513, 594, 1572
MunI	CAATTG	1 cut(s)	2441
Mva1269I	GAATGC	2 cut(s)	580, 2119
MvaI	CCWGG	3 cut(s)	198, 513, 1572
MwoI	GCNNNNNNNGC	3 cut(s)	856, 994, 2159
NciI	CCSGG	1 cut(s)	594
NdeI	CATATG	2 cut(s)	644, 2319
NdeII	GATC	7 cut(s)	145, 487, 1231, 1428, 1725, 1771, 2290
NlaIV	GGNNCC	4 cut(s)	454, 597, 1313, 1886
NmuCI	GTSAC	1 cut(s)	2019
NspI	RCATGY	4 cut(s)	9, 1741, 2020, 2226
PaeI	GCATGC	1 cut(s)	9
PaeR7I	CTCGAG	1 cut(s)	1861
PagI	TCATGA	3 cut(s)	91, 1635, 2233
PciI	ACATGT	2 cut(s)	2016, 2222
PctI	GAATGC	2 cut(s)	580, 2119
PdmI	GAANNNNTTC	1 cut(s)	2428
PfeI	GAWTC	5 cut(s)	1154, 1294, 1924, 1969, 2207
PflFI	GACNNNGTC	1 cut(s)	322
PflMI	CCANNNNNTGG	2 cut(s)	267, 882
PkrI	GCNGC	8 cut(s)	11, 35, 109, 1282, 1498, 1519, 2119, 2398
PleI	GAGTC	3 cut(s)	82, 361, 732
PpsI	GAGTC	3 cut(s)	82, 361, 732
PpuMI	RGGWCCY	1 cut(s)	1885
PscI	ACATGT	2 cut(s)	2016, 2222
PshAI	GACNNNNGTC	1 cut(s)	155
PsiI	TTATAA	3 cut(s)	807, 1557, 2099
Psp5II	RGGWCCY	1 cut(s)	1885
Psp6I	CCWGG	3 cut(s)	196, 511, 1570
PspFI	CCCAGC	2 cut(s)	449, 2284
PspGI	CCWGG	3 cut(s)	196, 511, 1570
PspN4I	GGNNCC	4 cut(s)	454, 597, 1313, 1886
PspPI	GGNCC	8 cut(s)	453, 596, 689, 926, 986, 1311, 1547, 1885
PspPPI	RGGWCCY	1 cut(s)	1885
PstI	CTGCAG	1 cut(s)	1519
PsuI	RGATCY	1 cut(s)	1231
PsyI	GACNNNGTC	1 cut(s)	322
PvuII	CAGCTG	3 cut(s)	110, 1283, 2288
RsaI	GTAC	7 cut(s)	342, 839, 1275, 1736, 1859, 2082, 2341
RsaNI	GTAC	7 cut(s)	341, 838, 1274, 1735, 1858, 2081, 2340
RseI	CAYNNNNRTG	1 cut(s)	1595
SalI	GTCGAC	1 cut(s)	2226
SaqAI	TTAA	5 cut(s)	348, 443, 1166, 1305, 2243
SatI	GCNGC	8 cut(s)	10, 34, 108, 1281, 1497, 1518, 2118, 2397
Sau3AI	GATC	7 cut(s)	145, 487, 1231, 1428, 1725, 1771, 2290
Sau96I	GGNCC	8 cut(s)	453, 596, 689, 926, 986, 1311, 1547, 1885
ScaI	AGTACT	3 cut(s)	342, 1859, 2082
SchI	GAGTC	3 cut(s)	82, 362, 733
ScrFI	CCNGG	4 cut(s)	198, 513, 594, 1572
SduI	GDGCHC	1 cut(s)	686
SexAI	ACCWGGT	1 cut(s)	196
SfaNI	GCATC	3 cut(s)	234, 1124, 1633
SfcI	CTRYAG	2 cut(s)	1515, 2310
Sfr274I	CTCGAG	1 cut(s)	1861
SinI	GGWCC	5 cut(s)	453, 596, 689, 1311, 1885
SlaI	CTCGAG	1 cut(s)	1861
SmiMI	CAYNNNNRTG	1 cut(s)	1595
SmlI	CTYRAG	4 cut(s)	83, 442, 721, 1861
SmoI	CTYRAG	4 cut(s)	83, 442, 721, 1861
SpeI	ACTAGT	1 cut(s)	1327
SphI	GCATGC	1 cut(s)	9
SsiI	CCGC	4 cut(s)	578, 1309, 2397, 2433
SspI	AATATT	1 cut(s)	1903
StyD4I	CCNGG	4 cut(s)	196, 511, 592, 1570
StyI	CCWWGG	1 cut(s)	692
TaaI	ACNGT	8 cut(s)	124, 288, 345, 675, 944, 2012, 2172, 2449
TaqI	TCGA	8 cut(s)	826, 1037, 1579, 1862, 1962, 2227, 2293, 2467
TatI	WGTACW	6 cut(s)	340, 837, 1734, 1857, 2080, 2339
TauI	GCSGC	1 cut(s)	2399
TfiI	GAWTC	5 cut(s)	1154, 1294, 1924, 1969, 2207
Tru1I	TTAA	5 cut(s)	348, 443, 1166, 1305, 2243
Tru9I	TTAA	5 cut(s)	348, 443, 1166, 1305, 2243
TscAI	CASTG	5 cut(s)	127, 691, 820, 1476, 2398
TseFI	GTSAC	1 cut(s)	2019
TseI	GCWGC	7 cut(s)	9, 33, 107, 1280, 1496, 1517, 2117
Tsp45I	GTSAC	1 cut(s)	2019
TspGWI	ACGGA	1 cut(s)	588
TspRI	CASTG	5 cut(s)	127, 691, 820, 1476, 2398
Tth111I	GACNNNGTC	1 cut(s)	322
Van91I	CCANNNNNTGG	2 cut(s)	267, 882
Vha464I	CTTAAG	1 cut(s)	442
VpaK11BI	GGWCC	5 cut(s)	453, 596, 689, 1311, 1885
XapI	RAATTY	7 cut(s)	22, 334, 950, 1760, 2137, 2386, 2425
XbaI	TCTAGA	2 cut(s)	1627, 2420
XceI	RCATGY	4 cut(s)	9, 1741, 2020, 2226
XcmI	CCANNNNNNNNNTGG	3 cut(s)	137, 268, 501
XhoI	CTCGAG	1 cut(s)	1861
XmiI	GTMKAC	2 cut(s)	374, 2227
XmnI	GAANNNNTTC	1 cut(s)	2428
ZrmI	AGTACT	3 cut(s)	342, 1859, 2082

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.