MD09G1230300.v1.1

Belongs to the glucose-6-phosphate 1-epimerase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0000789
Physical Location & Seq
Forward (+)
28531175 .. 28538538
7364 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1230300.v1.1.491

Sequence Viewer

Length: 735 bp
ATGGTCGTTCTCAGAAAGGTTCGAGGCTCTTCCGCGGAGGCATATTTGTATGGGGGTCAAGTTACATCATGGAAAAATGAATATGGGGAGGATTTGCTTTTCGTTAGTAGCAAGGCTACTTATAAGTCTCCTAAGGCTATCCGTGGTGGAATCCCAATATGTTTTCCTCAAGGCACATTTCCAGCAGTAAGATATGAATTTCGCCTTAGGGTAACTTTGGGACCTGGAGGGGATTTGATGTTGACATCTCGCATCCGAAATACAAACACTGATGGAAAGTCATTTACATTCACATTTGCCTATCACACCTATCTGGCTGTAACTGATATCAGTGAAGTGCGGGTAGAAGGACTGGAGACACTGGATTATCTGGATAACCTGAAGAGTAAAGAGCGATTTACTGAGCAAGGGGATGCAATAACATTTGAGTCTGAGGTGGACAAAGTTTATCTCAGCACGCCTACAAATATTGCTATTCTGGACCATGAAAGGAAGAGAACATTTGTGTTAAGGAAGGACAGGCTTCCAGATGCTGTTGTCTGGAATCCGTGGGATAAGAAAGCAAAGGCAATTACTGATTTTGGGGATGATGAGTACAAGAATATGCTGTGTGTCCAGGCTGCTTGTGTGGAAAAGCCTGTTGTAGAATGGAAAGGAAGGCCGGAACTGTCTGCCGTCCCTTCCAGTTACTGCCGCGGACAACTTGACCCACGGAAGGTACTGCTAGGCGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00010 ko01100 ko01110 ko01120 ko01130 map00010 map01100 map01110 map01120 map01130
Pfam Domains
Protein Families
Aldose_epim

Protein Analysis

245

Amino Acids

27.54

Weight (kDa)

8.55

Isoelectric Point (pI)

27.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldose_epim PF01263 2 - 57 3.5e-07 Aldose 1-epimerase
Aldose_epim PF01263 64 - 214 1.7e-31 Aldose 1-epimerase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 123
AccII CGCG 2 cut(s) 35, 696
AciI CCGC 6 cut(s) 33, 35, 340, 694, 696, 729
AcsI RAATTY 1 cut(s) 197
AcuI CTGAAG 1 cut(s) 401
AfaI GTAC 2 cut(s) 596, 720
AfiI CCNNNNNNNGG 1 cut(s) 715
AjnI CCWGG 2 cut(s) 223, 615
Alw26I GTCTC 2 cut(s) 132, 350
AlwNI CAGNNNCTG 2 cut(s) 533, 690
AoxI GGCC 1 cut(s) 659
ApeKI GCWGC 1 cut(s) 620
ApoI RAATTY 1 cut(s) 197
AspS9I GGNCC 2 cut(s) 221, 481
AvaII GGWCC 2 cut(s) 221, 481
AxyI CCTNAGG 2 cut(s) 132, 206
BbvI GCAGC 1 cut(s) 607
BccI CCATC 1 cut(s) 266
BceAI ACGGC 1 cut(s) 659
BciT130I CCWGG 2 cut(s) 225, 617
BcoDI GTCTC 2 cut(s) 132, 350
BfaI CTAG 1 cut(s) 725
BisI GCNGC 2 cut(s) 621, 694
BlsI GCNGC 2 cut(s) 622, 695
Bme1390I CCNGG 2 cut(s) 225, 617
Bme18I GGWCC 2 cut(s) 221, 481
BmgT120I GGNCC 2 cut(s) 221, 481
BmiI GGNNCC 1 cut(s) 222
BmrFI CCNGG 2 cut(s) 225, 617
BmsI GCATC 3 cut(s) 261, 403, 520
BpmI CTGGAG 2 cut(s) 246, 374
BpuEI CTTGAG 1 cut(s) 153
BsaJI CCNNGG 5 cut(s) 33, 142, 548, 694, 710
Bsc4I CCNNNNNNNGG 1 cut(s) 715
Bse1I ACTGG 3 cut(s) 357, 366, 684
Bse21I CCTNAGG 2 cut(s) 132, 206
BseBI CCWGG 2 cut(s) 225, 617
BseDI CCNNGG 5 cut(s) 33, 142, 548, 694, 710
BseGI GGATG 3 cut(s) 252, 418, 592
BseLI CCNNNNNNNGG 1 cut(s) 715
BseMII CTCAG 4 cut(s) 25, 393, 423, 466
BseNI ACTGG 3 cut(s) 357, 366, 684
BseXI GCAGC 1 cut(s) 607
Bsh1236I CGCG 2 cut(s) 35, 696
BshFI GGCC 1 cut(s) 661
BsiSI CCGG 1 cut(s) 662
BslFI GGGAC 2 cut(s) 234, 662
BslI CCNNNNNNNGG 1 cut(s) 715
BsmAI GTCTC 2 cut(s) 132, 350
BsmFI GGGAC 2 cut(s) 234, 662
BsnI GGCC 1 cut(s) 661
BspACI CCGC 6 cut(s) 33, 35, 340, 694, 696, 729
BspANI GGCC 1 cut(s) 661
BspCNI CTCAG 4 cut(s) 24, 394, 424, 465
BspFNI CGCG 2 cut(s) 35, 696
BspLI GGNNCC 1 cut(s) 222
BspQI GCTCTTC 1 cut(s) 34
BsrI ACTGG 3 cut(s) 357, 366, 684
BssECI CCNNGG 5 cut(s) 33, 142, 548, 694, 710
Bst2UI CCWGG 2 cut(s) 225, 617
Bst4CI ACNGT 1 cut(s) 669
Bst6I CTCTTC 3 cut(s) 34, 377, 488
BstC8I GCNNGC 1 cut(s) 458
BstDEI CTNAG 6 cut(s) 11, 132, 206, 402, 432, 452
BstDSI CCRYGG 5 cut(s) 33, 142, 548, 694, 710
BstF5I GGATG 3 cut(s) 252, 418, 592
BstFNI CGCG 2 cut(s) 35, 696
BstMAI GTCTC 2 cut(s) 132, 350
BstNI CCWGG 2 cut(s) 225, 617
BstSCI CCNGG 2 cut(s) 223, 615
BstUI CGCG 2 cut(s) 35, 696
BstV1I GCAGC 1 cut(s) 607
Bsu36I CCTNAGG 2 cut(s) 132, 206
BsuRI GGCC 1 cut(s) 661
BtgI CCRYGG 5 cut(s) 33, 142, 548, 694, 710
BtsCI GGATG 3 cut(s) 252, 418, 592
BtsIMutI CAGTG 3 cut(s) 267, 337, 359
Cac8I GCNNGC 1 cut(s) 458
CaiI CAGNNNCTG 2 cut(s) 533, 690
Cfr13I GGNCC 2 cut(s) 221, 481
Cfr42I CCGCGG 2 cut(s) 36, 697
Csp6I GTAC 2 cut(s) 595, 719
CviAII CATG 2 cut(s) 69, 485
CviJI RGCY 8 cut(s) 27, 116, 137, 317, 523, 620, 637, 661
CviKI_1 RGCY 8 cut(s) 27, 116, 137, 317, 523, 620, 637, 661
CviQI GTAC 2 cut(s) 595, 719
DdeI CTNAG 6 cut(s) 11, 132, 206, 402, 432, 452
Eam1104I CTCTTC 3 cut(s) 34, 377, 488
EarI CTCTTC 3 cut(s) 34, 377, 488
Eco32I GATATC 1 cut(s) 328
Eco47I GGWCC 2 cut(s) 221, 481
Eco57I CTGAAG 1 cut(s) 401
Eco81I CCTNAGG 2 cut(s) 132, 206
EcoO109I RGGNCCY 1 cut(s) 221
EcoRII CCWGG 2 cut(s) 223, 615
EcoRV GATATC 1 cut(s) 328
FaeI CATG 2 cut(s) 72, 488
FaiI YATR 9 cut(s) 43, 51, 70, 84, 123, 160, 195, 486, 605
FaqI GGGAC 2 cut(s) 234, 662
FatI CATG 2 cut(s) 68, 484
FauI CCCGC 1 cut(s) 333
Fnu4HI GCNGC 2 cut(s) 621, 694
FokI GGATG 3 cut(s) 239, 425, 599
Fsp4HI GCNGC 2 cut(s) 621, 694
FspBI CTAG 1 cut(s) 725
GluI GCNGC 2 cut(s) 621, 694
GsuI CTGGAG 2 cut(s) 246, 374
HaeIII GGCC 1 cut(s) 661
HapII CCGG 1 cut(s) 662
Hin1II CATG 2 cut(s) 72, 488
HincII GTYRAC 1 cut(s) 243
HindII GTYRAC 1 cut(s) 243
HinfI GANTC 3 cut(s) 150, 428, 544
HpaII CCGG 1 cut(s) 662
Hpy166II GTNNAC 2 cut(s) 243, 439
Hpy188I TCNGA 3 cut(s) 14, 257, 433
Hpy188III TCNNGA 4 cut(s) 371, 479, 527, 541
Hpy8I GTNNAC 2 cut(s) 243, 439
HpyAV CCTTC 5 cut(s) 341, 508, 651, 690, 709
HpyCH4III ACNGT 1 cut(s) 669
HpyCH4V TGCA 1 cut(s) 416
HpyF3I CTNAG 6 cut(s) 11, 132, 206, 402, 432, 452
Hsp92II CATG 2 cut(s) 72, 488
KspI CCGCGG 2 cut(s) 36, 697
LguI GCTCTTC 1 cut(s) 34
Lsp1109I GCAGC 1 cut(s) 607
LweI GCATC 3 cut(s) 261, 403, 520
MaeI CTAG 1 cut(s) 725
MaeIII GTNAC 4 cut(s) 61, 211, 319, 686
MboII GAAGA 3 cut(s) 21, 394, 505
MluCI AATT 2 cut(s) 197, 570
MlyI GAGTC 1 cut(s) 437
MnlI CCTC 6 cut(s) 17, 31, 82, 177, 221, 427
MseI TTAA 2 cut(s) 509, 733
MspA1I CMGCKG 2 cut(s) 35, 696
MspI CCGG 1 cut(s) 662
MspR9I CCNGG 2 cut(s) 225, 617
MvaI CCWGG 2 cut(s) 225, 617
MvnI CGCG 2 cut(s) 35, 696
NlaIII CATG 2 cut(s) 72, 488
NlaIV GGNNCC 1 cut(s) 222
PciSI GCTCTTC 1 cut(s) 34
PfeI GAWTC 2 cut(s) 150, 544
PkrI GCNGC 2 cut(s) 622, 695
PleI GAGTC 1 cut(s) 436
PpsI GAGTC 1 cut(s) 436
PpuMI RGGWCCY 1 cut(s) 221
PsiI TTATAA 1 cut(s) 123
Psp5II RGGWCCY 1 cut(s) 221
Psp6I CCWGG 2 cut(s) 223, 615
PspGI CCWGG 2 cut(s) 223, 615
PspN4I GGNNCC 1 cut(s) 222
PspPI GGNCC 2 cut(s) 221, 481
PspPPI RGGWCCY 1 cut(s) 221
PstNI CAGNNNCTG 2 cut(s) 533, 690
RsaI GTAC 2 cut(s) 596, 720
RsaNI GTAC 2 cut(s) 595, 719
SacII CCGCGG 2 cut(s) 36, 697
SapI GCTCTTC 1 cut(s) 34
SaqAI TTAA 2 cut(s) 509, 733
SatI GCNGC 2 cut(s) 621, 694
Sau96I GGNCC 2 cut(s) 221, 481
SchI GAGTC 1 cut(s) 437
ScrFI CCNGG 2 cut(s) 225, 617
SetI ASST 6 cut(s) 21, 226, 311, 381, 438, 720
SfaNI GCATC 3 cut(s) 261, 403, 520
Sfr303I CCGCGG 2 cut(s) 36, 697
SgrBI CCGCGG 2 cut(s) 36, 697
SinI GGWCC 2 cut(s) 221, 481
SmlI CTYRAG 1 cut(s) 168
SmoI CTYRAG 1 cut(s) 168
Sse9I AATT 2 cut(s) 197, 570
SsiI CCGC 6 cut(s) 33, 35, 340, 694, 696, 729
SspI AATATT 1 cut(s) 469
SspMI CTAG 1 cut(s) 725
StyD4I CCNGG 2 cut(s) 223, 615
TaaI ACNGT 1 cut(s) 669
TaqI TCGA 1 cut(s) 22
TasI AATT 2 cut(s) 197, 570
TatI WGTACW 1 cut(s) 594
TauI GCSGC 1 cut(s) 696
TfiI GAWTC 2 cut(s) 150, 544
Tru1I TTAA 2 cut(s) 509, 733
Tru9I TTAA 2 cut(s) 509, 733
TscAI CASTG 3 cut(s) 274, 337, 366
TseI GCWGC 1 cut(s) 620
TspDTI ATGAA 3 cut(s) 93, 210, 501
TspGWI ACGGA 3 cut(s) 131, 537, 727
TspRI CASTG 3 cut(s) 274, 337, 366
VpaK11BI GGWCC 2 cut(s) 221, 481
XapI RAATTY 1 cut(s) 197
XspI CTAG 1 cut(s) 725
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.