MD17G1229200.v1.1

Belongs to the glucose-6-phosphate 1-epimerase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0000789
Physical Location & Seq
Forward (+)
27675709 .. 27687368
11660 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1229200.v1.1.491

Sequence Viewer

Length: 1044 bp
ATGTCTTCCGGCGTCCGGGAGAGAGAGAGGGTGTTTGTGGAGCACTGTAAGGGCGCGAATGGCCTTAACAAGGTCGTCCTCAGAGAGGTTCGAGGCTACTCCGCGGAGGTATATTTGTATGGGGGTCAAGTTACATCATGGAGAAATGAATATGGGGAAGATTTGCTTTTCGTTAGTAGCAAGGCTACTTATAAGTCTCCTAAGGCTATCCGTGGCGGAATCCCAATATGTTTTCCTCAATTTGGAAGTCATGGTTCTCTTGAACAACATGGATTTGCAAGGAATAGACTTTGGAGTATTGATCACGATCCCCCGCCTTTCCCAACAAACAGCGGTAACAAGGCTTTCATTGATTTAATCCTCAAACCCTCTGAAGAGGATGCAAAGATCTGGCCTCATAGATATGAATTTCGCCTTAGGGTAACTTTGGGACCAGGAGGGGATTTGATGTTGACTTCTCGCATCCGAAATACAAACACTGATGGAAGGTCGTTTACATTCACATTTGCCTATCACACCTATCTGGCCGTAACTGATATCAGCGAAGTGCGGGTAGAAGGACTGGAGACACGGGATTATCTGGATAACCTGAAGAATAGAGAGCGATTTACTGAGCAAGGGGATGCAATAACATTTGAGTCTGAGGTGGACAAAGTTTATCTCAGCACGCCTACAAAAATTGCTATTCTGGACCATGAAAGGAAGAGAACATTTGTGTTACGGAAGGACGGGCTTCCAGATGCTGTTGTCTGGAATCCGTGGGATAAGAAAGCAAAGGCAATTACTGATTTGGGGGATGATGAGTACAAGCATATGCTGTGTGTCCAGGCTGCTTGTGTGGAAAAGCCTATTACTTTGAAACCTGGTGAAGAATGGAAAGGAAGGCAGGAACTGTCTGCTGTCCCTTCCAGTTACTGCAGTGGACAACTTGACCCACGGAAGGCACATTTTGCATTCGAGGTTTGGCAGGATTTCTCGGTTGGATGGCAGATTCATTACAAAGCCGAATTTGTCTATAAGGAGTTTCATCGGATATTTGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00010 ko01100 ko01110 ko01120 ko01130 map00010 map01100 map01110 map01120 map01130
Pfam Domains
Protein Families
Aldose_epim

Protein Analysis

348

Amino Acids

39.9

Weight (kDa)

6.84

Isoelectric Point (pI)

29.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldose_epim PF01263 25 - 298 9.7e-65 Aldose 1-epimerase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 192
AccII CGCG 2 cut(s) 56, 104
AciI CCGC 6 cut(s) 102, 104, 216, 314, 333, 550
AclWI GGATC 1 cut(s) 302
AcoI YGGCCR 1 cut(s) 525
AcsI RAATTY 2 cut(s) 407, 1007
AcuI CTGAAG 2 cut(s) 393, 611
AcyI GRCGYC 1 cut(s) 12
AfaI GTAC 1 cut(s) 806
AfiI CCNNNNNNNGG 5 cut(s) 15, 70, 85, 242, 940
AgsI TTSAA 2 cut(s) 263, 859
AjnI CCWGG 3 cut(s) 433, 825, 862
Alw21I GWGCWC 1 cut(s) 45
Alw26I GTCTC 2 cut(s) 201, 560
AlwI GGATC 1 cut(s) 302
AlwNI CAGNNNCTG 3 cut(s) 743, 892, 915
AoxI GGCC 3 cut(s) 61, 392, 525
ApeKI GCWGC 1 cut(s) 830
ApoI RAATTY 2 cut(s) 407, 1007
AspLEI GCGC 1 cut(s) 56
AspS9I GGNCC 2 cut(s) 431, 691
AsuC2I CCSGG 1 cut(s) 17
AsuHPI GGTGA 1 cut(s) 878
AvaII GGWCC 2 cut(s) 431, 691
AxyI CCTNAGG 2 cut(s) 201, 416
BarI GAAGNNNNNNTAC 2 cut(s) 478, 510
Bbv12I GWGCWC 1 cut(s) 45
BbvI GCAGC 1 cut(s) 817
BccI CCATC 2 cut(s) 476, 978
BceAI ACGGC 1 cut(s) 512
BciT130I CCWGG 3 cut(s) 435, 827, 864
BclI TGATCA 1 cut(s) 301
BcnI CCSGG 1 cut(s) 17
BcoDI GTCTC 2 cut(s) 201, 560
BfmI CTRYAG 1 cut(s) 916
BglII AGATCT 1 cut(s) 387
BisI GCNGC 1 cut(s) 831
BlsI GCNGC 1 cut(s) 832
Bme1390I CCNGG 4 cut(s) 17, 435, 827, 864
Bme18I GGWCC 2 cut(s) 431, 691
BmgT120I GGNCC 2 cut(s) 431, 691
BmiI GGNNCC 1 cut(s) 432
BmrFI CCNGG 4 cut(s) 17, 435, 827, 864
BmsI GCATC 4 cut(s) 370, 471, 613, 730
BpmI CTGGAG 1 cut(s) 584
BpuMI CCSGG 1 cut(s) 17
BsaBI GATNNNNATC 1 cut(s) 306
BsaHI GRCGYC 1 cut(s) 12
BsaJI CCNNGG 4 cut(s) 102, 211, 758, 935
Bsc4I CCNNNNNNNGG 5 cut(s) 15, 70, 85, 242, 940
Bse1I ACTGG 2 cut(s) 567, 909
Bse21I CCTNAGG 2 cut(s) 201, 416
Bse8I GATNNNNATC 1 cut(s) 306
BseBI CCWGG 3 cut(s) 435, 827, 864
BseDI CCNNGG 4 cut(s) 102, 211, 758, 935
BseGI GGATG 5 cut(s) 385, 462, 628, 802, 989
BseJI GATNNNNATC 1 cut(s) 306
BseLI CCNNNNNNNGG 5 cut(s) 15, 70, 85, 242, 940
BseMII CTCAG 4 cut(s) 94, 603, 633, 676
BseNI ACTGG 2 cut(s) 567, 909
BseXI GCAGC 1 cut(s) 817
Bsh1236I CGCG 2 cut(s) 56, 104
BshFI GGCC 3 cut(s) 63, 394, 527
BsiHKAI GWGCWC 1 cut(s) 45
BsiSI CCGG 2 cut(s) 9, 16
BslFI GGGAC 2 cut(s) 444, 887
BslI CCNNNNNNNGG 5 cut(s) 15, 70, 85, 242, 940
BsmAI GTCTC 2 cut(s) 201, 560
BsmFI GGGAC 2 cut(s) 444, 887
BsmI GAATGC 1 cut(s) 953
BsnI GGCC 3 cut(s) 63, 394, 527
Bsp1286I GDGCHC 1 cut(s) 45
Bsp143I GATC 3 cut(s) 301, 307, 387
BspACI CCGC 6 cut(s) 102, 104, 216, 314, 333, 550
BspANI GGCC 3 cut(s) 63, 394, 527
BspCNI CTCAG 4 cut(s) 93, 604, 634, 675
BspFNI CGCG 2 cut(s) 56, 104
BspLI GGNNCC 1 cut(s) 432
BspMAI CTGCAG 1 cut(s) 920
BspPI GGATC 1 cut(s) 302
BsrI ACTGG 2 cut(s) 567, 909
BssECI CCNNGG 4 cut(s) 102, 211, 758, 935
BssMI GATC 3 cut(s) 301, 307, 387
BssNI GRCGYC 1 cut(s) 12
Bst2UI CCWGG 3 cut(s) 435, 827, 864
Bst4CI ACNGT 2 cut(s) 47, 894
Bst6I CTCTTC 2 cut(s) 369, 698
BstACI GRCGYC 1 cut(s) 12
BstAPI GCANNNNNTGC 1 cut(s) 950
BstC8I GCNNGC 1 cut(s) 668
BstDEI CTNAG 6 cut(s) 80, 201, 416, 612, 642, 662
BstDSI CCRYGG 4 cut(s) 102, 211, 758, 935
BstENI CCTNNNNNAGG 2 cut(s) 68, 83
BstF5I GGATG 5 cut(s) 385, 462, 628, 802, 989
BstFNI CGCG 2 cut(s) 56, 104
BstHHI GCGC 1 cut(s) 56
BstKTI GATC 3 cut(s) 304, 310, 390
BstMAI GTCTC 2 cut(s) 201, 560
BstMBI GATC 3 cut(s) 301, 307, 387
BstMWI GCNNNNNNNGC 2 cut(s) 60, 950
BstNI CCWGG 3 cut(s) 435, 827, 864
BstSCI CCNGG 4 cut(s) 15, 433, 825, 862
BstSFI CTRYAG 1 cut(s) 916
BstUI CGCG 2 cut(s) 56, 104
BstV1I GCAGC 1 cut(s) 817
BstX2I RGATCY 1 cut(s) 387
BstYI RGATCY 1 cut(s) 387
Bsu36I CCTNAGG 2 cut(s) 201, 416
BsuRI GGCC 3 cut(s) 63, 394, 527
BtgI CCRYGG 4 cut(s) 102, 211, 758, 935
BtsCI GGATG 5 cut(s) 385, 462, 628, 802, 989
BtsI GCAGTG 1 cut(s) 925
BtsIMutI CAGTG 3 cut(s) 43, 477, 925
Cac8I GCNNGC 1 cut(s) 668
CaiI CAGNNNCTG 3 cut(s) 743, 892, 915
CfoI GCGC 1 cut(s) 56
Cfr13I GGNCC 2 cut(s) 431, 691
Cfr42I CCGCGG 1 cut(s) 105
CsiI ACCWGGT 1 cut(s) 862
Csp6I GTAC 1 cut(s) 805
CviAII CATG 4 cut(s) 138, 251, 269, 695
CviQI GTAC 1 cut(s) 805
DdeI CTNAG 6 cut(s) 80, 201, 416, 612, 642, 662
DpnI GATC 3 cut(s) 303, 309, 389
DpnII GATC 3 cut(s) 301, 307, 387
EaeI YGGCCR 1 cut(s) 525
Eam1104I CTCTTC 2 cut(s) 369, 698
EarI CTCTTC 2 cut(s) 369, 698
EciI GGCGGA 1 cut(s) 231
Eco32I GATATC 1 cut(s) 538
Eco47I GGWCC 2 cut(s) 431, 691
Eco57I CTGAAG 2 cut(s) 393, 611
Eco81I CCTNAGG 2 cut(s) 201, 416
EcoNI CCTNNNNNAGG 2 cut(s) 68, 83
EcoRII CCWGG 3 cut(s) 433, 825, 862
EcoRV GATATC 1 cut(s) 538
FaeI CATG 4 cut(s) 141, 254, 272, 698
FalI AAGNNNNNCTT 2 cut(s) 150, 182
FaqI GGGAC 2 cut(s) 444, 887
FatI CATG 4 cut(s) 137, 250, 268, 694
FauI CCCGC 2 cut(s) 321, 543
FauNDI CATATG 1 cut(s) 813
FbaI TGATCA 1 cut(s) 301
Fnu4HI GCNGC 1 cut(s) 831
FokI GGATG 5 cut(s) 392, 449, 635, 809, 996
Fsp4HI GCNGC 1 cut(s) 831
GlaI GCGC 1 cut(s) 55
GluI GCNGC 1 cut(s) 831
GsuI CTGGAG 1 cut(s) 584
HaeIII GGCC 3 cut(s) 63, 394, 527
HapII CCGG 2 cut(s) 9, 16
HhaI GCGC 1 cut(s) 56
Hin1I GRCGYC 1 cut(s) 12
Hin1II CATG 4 cut(s) 141, 254, 272, 698
Hin6I GCGC 1 cut(s) 54
HinP1I GCGC 1 cut(s) 54
HincII GTYRAC 1 cut(s) 453
HindII GTYRAC 1 cut(s) 453
HinfI GANTC 4 cut(s) 219, 638, 754, 991
HpaII CCGG 2 cut(s) 9, 16
HphI GGTGA 1 cut(s) 878
Hpy166II GTNNAC 4 cut(s) 453, 495, 649, 923
Hpy188I TCNGA 5 cut(s) 83, 373, 467, 643, 1032
Hpy188III TCNNGA 6 cut(s) 260, 305, 581, 689, 737, 751
Hpy8I GTNNAC 4 cut(s) 453, 495, 649, 923
HpyAV CCTTC 6 cut(s) 480, 551, 718, 876, 915, 934
HpyCH4III ACNGT 2 cut(s) 47, 894
HpyCH4V TGCA 5 cut(s) 278, 383, 626, 918, 953
HpyF10VI GCNNNNNNNGC 2 cut(s) 60, 950
HpyF3I CTNAG 6 cut(s) 80, 201, 416, 612, 642, 662
Hsp92I GRCGYC 1 cut(s) 12
Hsp92II CATG 4 cut(s) 141, 254, 272, 698
HspAI GCGC 1 cut(s) 54
Ksp22I TGATCA 1 cut(s) 301
KspI CCGCGG 1 cut(s) 105
Kzo9I GATC 3 cut(s) 301, 307, 387
LmnI GCTCC 1 cut(s) 40
Lsp1109I GCAGC 1 cut(s) 817
LweI GCATC 4 cut(s) 370, 471, 613, 730
MabI ACCWGGT 1 cut(s) 862
MaeIII GTNAC 6 cut(s) 130, 335, 421, 529, 717, 911
MalI GATC 3 cut(s) 303, 309, 389
MboI GATC 3 cut(s) 301, 307, 387
MboII GAAGA 5 cut(s) 170, 386, 604, 715, 881
MflI RGATCY 1 cut(s) 387
MhlI GDGCHC 1 cut(s) 45
MluCI AATT 5 cut(s) 239, 407, 678, 780, 1007
MlyI GAGTC 1 cut(s) 647
MmeI TCCRAC 1 cut(s) 961
MseI TTAA 3 cut(s) 66, 356, 1042
MslI CAYNNNNRTG 1 cut(s) 402
MspA1I CMGCKG 2 cut(s) 104, 333
MspI CCGG 2 cut(s) 9, 16
MspR9I CCNGG 4 cut(s) 17, 435, 827, 864
Mva1269I GAATGC 1 cut(s) 953
MvaI CCWGG 3 cut(s) 435, 827, 864
MvnI CGCG 2 cut(s) 56, 104
MwoI GCNNNNNNNGC 2 cut(s) 60, 950
NciI CCSGG 1 cut(s) 17
NdeI CATATG 1 cut(s) 813
NdeII GATC 3 cut(s) 301, 307, 387
NlaIII CATG 4 cut(s) 141, 254, 272, 698
NlaIV GGNNCC 1 cut(s) 432
PctI GAATGC 1 cut(s) 953
PfeI GAWTC 3 cut(s) 219, 754, 991
PfoI TCCNGGA 1 cut(s) 15
PkrI GCNGC 1 cut(s) 832
PleI GAGTC 1 cut(s) 646
PpsI GAGTC 1 cut(s) 646
PsiI TTATAA 1 cut(s) 192
Psp6I CCWGG 3 cut(s) 433, 825, 862
PspGI CCWGG 3 cut(s) 433, 825, 862
PspN4I GGNNCC 1 cut(s) 432
PspPI GGNCC 2 cut(s) 431, 691
PstI CTGCAG 1 cut(s) 920
PstNI CAGNNNCTG 3 cut(s) 743, 892, 915
PsuI RGATCY 1 cut(s) 387
RsaI GTAC 1 cut(s) 806
RsaNI GTAC 1 cut(s) 805
RseI CAYNNNNRTG 1 cut(s) 402
SacII CCGCGG 1 cut(s) 105
SaqAI TTAA 3 cut(s) 66, 356, 1042
SatI GCNGC 1 cut(s) 831
Sau3AI GATC 3 cut(s) 301, 307, 387
Sau96I GGNCC 2 cut(s) 431, 691
SchI GAGTC 1 cut(s) 647
ScrFI CCNGG 4 cut(s) 17, 435, 827, 864
SduI GDGCHC 1 cut(s) 45
SetI ASST 9 cut(s) 75, 90, 111, 491, 521, 591, 648, 865, 963
SexAI ACCWGGT 1 cut(s) 862
SfaNI GCATC 4 cut(s) 370, 471, 613, 730
SfcI CTRYAG 1 cut(s) 916
Sfr303I CCGCGG 1 cut(s) 105
SgrBI CCGCGG 1 cut(s) 105
SinI GGWCC 2 cut(s) 431, 691
SmiMI CAYNNNNRTG 1 cut(s) 402
Sse9I AATT 5 cut(s) 239, 407, 678, 780, 1007
SsiI CCGC 6 cut(s) 102, 104, 216, 314, 333, 550
StyD4I CCNGG 4 cut(s) 15, 433, 825, 862
TaaI ACNGT 2 cut(s) 47, 894
TaqI TCGA 2 cut(s) 91, 957
TasI AATT 5 cut(s) 239, 407, 678, 780, 1007
TatI WGTACW 1 cut(s) 804
TfiI GAWTC 3 cut(s) 219, 754, 991
Tru1I TTAA 3 cut(s) 66, 356, 1042
Tru9I TTAA 3 cut(s) 66, 356, 1042
TscAI CASTG 3 cut(s) 50, 484, 925
TseI GCWGC 1 cut(s) 830
TspDTI ATGAA 6 cut(s) 162, 337, 420, 711, 983, 1016
TspGWI ACGGA 4 cut(s) 200, 736, 747, 952
TspRI CASTG 3 cut(s) 50, 484, 925
VpaK11BI GGWCC 2 cut(s) 431, 691
XagI CCTNNNNNAGG 2 cut(s) 68, 83
XapI RAATTY 2 cut(s) 407, 1007
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.