pycom14g13110

Purple acid phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0000789
Physical Location & Seq
Forward (+)
16108508 .. 16109348
841 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g13110.2

Sequence Viewer

Length: 390 bp
ATGTTCGGTGACATGGGCAAGGGAGAGCGTGACGGTTCAAATGAGTACAGTAATTACCATCCTGGTGCACTGATGACTACGGATCAAATCATCAGCGATTTGGAGAACGTCGACATTGTTTTTGATATTGGAAATCTAACGTATGCAAAGGGTACATCTCACAATGGGACCAGTTCACGGCACAGGTGGAACCGATTGCATCAACAGTGCCATATATGGTTGGCAGTGGTAATCATGAGCGAGATTGGCCTGATTCGGGGTCCTTTTATGACAAAATGGATTCTGGTGGAGAATGTGGCGTGTTGGCTGAGACTATGTTCTATGTTCCAGCCGAGAACCGAGCAAAGTTCTGGTACAAAACCGACTATGGCATGTATCGCTTCTGCATAG

Protein Analysis

130

Amino Acids

14.49

Weight (kDa)

6.49

Isoelectric Point (pI)

52.66

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 111
AclWI GGATC 1 cut(s) 90
AfaI GTAC 3 cut(s) 47, 154, 355
AfiI CCNNNNNNNGG 2 cut(s) 177, 256
AgsI TTSAA 1 cut(s) 39
AjnI CCWGG 1 cut(s) 61
Alw21I GWGCWC 1 cut(s) 70
Alw26I GTCTC 1 cut(s) 304
Alw44I GTGCAC 1 cut(s) 66
AlwI GGATC 1 cut(s) 90
AoxI GGCC 1 cut(s) 247
ApaLI GTGCAC 1 cut(s) 66
ArsI GACNNNNNNTTYG 2 cut(s) 104, 136
AspS9I GGNCC 2 cut(s) 168, 260
AsuHPI GGTGA 1 cut(s) 20
AvaII GGWCC 2 cut(s) 168, 260
BaeGI GKGCMC 1 cut(s) 70
Bbv12I GWGCWC 1 cut(s) 70
BccI CCATC 1 cut(s) 66
BceAI ACGGC 1 cut(s) 194
BciT130I CCWGG 1 cut(s) 63
BcoDI GTCTC 1 cut(s) 304
Bme1390I CCNGG 1 cut(s) 63
Bme18I GGWCC 2 cut(s) 168, 260
BmgT120I GGNCC 2 cut(s) 168, 260
BmiI GGNNCC 3 cut(s) 169, 191, 261
BmrFI CCNGG 1 cut(s) 63
BmsI GCATC 1 cut(s) 208
Bsc4I CCNNNNNNNGG 2 cut(s) 177, 256
Bse1I ACTGG 1 cut(s) 171
BseBI CCWGG 1 cut(s) 63
BseGI GGATG 1 cut(s) 58
BseLI CCNNNNNNNGG 2 cut(s) 177, 256
BseMII CTCAG 1 cut(s) 299
BseNI ACTGG 1 cut(s) 171
BseSI GKGCMC 1 cut(s) 70
BshFI GGCC 1 cut(s) 249
BsiHKAI GWGCWC 1 cut(s) 70
BslFI GGGAC 1 cut(s) 181
BslI CCNNNNNNNGG 2 cut(s) 177, 256
BsmAI GTCTC 1 cut(s) 304
BsmFI GGGAC 1 cut(s) 181
BsnI GGCC 1 cut(s) 249
Bsp1286I GDGCHC 1 cut(s) 70
Bsp143I GATC 1 cut(s) 82
BspANI GGCC 1 cut(s) 249
BspCNI CTCAG 1 cut(s) 300
BspHI TCATGA 1 cut(s) 234
BspLI GGNNCC 3 cut(s) 169, 191, 261
BspPI GGATC 1 cut(s) 90
BsrI ACTGG 1 cut(s) 171
BssMI GATC 1 cut(s) 82
Bst2UI CCWGG 1 cut(s) 63
Bst4CI ACNGT 3 cut(s) 35, 50, 207
BstDEI CTNAG 1 cut(s) 308
BstF5I GGATG 1 cut(s) 58
BstKTI GATC 1 cut(s) 85
BstMAI GTCTC 1 cut(s) 304
BstMBI GATC 1 cut(s) 82
BstMWI GCNNNNNNNGC 2 cut(s) 246, 377
BstNI CCWGG 1 cut(s) 63
BstNSI RCATGY 1 cut(s) 375
BstSCI CCNGG 1 cut(s) 61
BstSLI GKGCMC 1 cut(s) 70
BsuRI GGCC 1 cut(s) 249
BtsCI GGATG 1 cut(s) 58
BtsI GCAGTG 1 cut(s) 231
BtsIMutI CAGTG 3 cut(s) 68, 212, 231
CciI TCATGA 1 cut(s) 234
Cfr13I GGNCC 2 cut(s) 168, 260
Csp6I GTAC 3 cut(s) 46, 153, 354
CviAII CATG 3 cut(s) 13, 235, 372
CviJI RGCY 3 cut(s) 249, 307, 331
CviKI_1 RGCY 3 cut(s) 249, 307, 331
CviQI GTAC 3 cut(s) 46, 153, 354
DdeI CTNAG 1 cut(s) 308
DpnI GATC 1 cut(s) 84
DpnII GATC 1 cut(s) 82
Eco47I GGWCC 2 cut(s) 168, 260
EcoO109I RGGNCCY 1 cut(s) 260
EcoRII CCWGG 1 cut(s) 61
FaeI CATG 3 cut(s) 16, 238, 375
FaqI GGGAC 1 cut(s) 181
FatI CATG 3 cut(s) 12, 234, 371
FblI GTMKAC 1 cut(s) 111
FokI GGATG 1 cut(s) 45
HaeIII GGCC 1 cut(s) 249
Hin1II CATG 3 cut(s) 16, 238, 375
HincII GTYRAC 1 cut(s) 112
HindII GTYRAC 1 cut(s) 112
HinfI GANTC 2 cut(s) 253, 280
HphI GGTGA 1 cut(s) 20
Hpy166II GTNNAC 3 cut(s) 68, 112, 176
Hpy188III TCNNGA 1 cut(s) 235
Hpy8I GTNNAC 3 cut(s) 68, 112, 176
Hpy99I CGWCG 1 cut(s) 113
HpyCH4III ACNGT 3 cut(s) 35, 50, 207
HpyCH4IV ACGT 2 cut(s) 108, 140
HpyCH4V TGCA 4 cut(s) 68, 146, 199, 386
HpyF10VI GCNNNNNNNGC 2 cut(s) 246, 377
HpyF3I CTNAG 1 cut(s) 308
HpySE526I ACGT 2 cut(s) 108, 140
Hsp92II CATG 3 cut(s) 16, 238, 375
Kzo9I GATC 1 cut(s) 82
LpnPI CCDG 8 cut(s) 48, 75, 169, 184, 263, 269, 336, 341
LweI GCATC 1 cut(s) 208
MaeII ACGT 2 cut(s) 108, 140
MaeIII GTNAC 2 cut(s) 8, 29
MalI GATC 1 cut(s) 84
MboI GATC 1 cut(s) 82
MhlI GDGCHC 1 cut(s) 70
MluCI AATT 1 cut(s) 52
MslI CAYNNNNRTG 1 cut(s) 63
MspR9I CCNGG 1 cut(s) 63
MvaI CCWGG 1 cut(s) 63
MwoI GCNNNNNNNGC 2 cut(s) 246, 377
NdeII GATC 1 cut(s) 82
NlaIII CATG 3 cut(s) 16, 238, 375
NlaIV GGNNCC 3 cut(s) 169, 191, 261
NmeAIII GCCGAG 1 cut(s) 357
NmuCI GTSAC 2 cut(s) 8, 29
NspI RCATGY 1 cut(s) 375
PagI TCATGA 1 cut(s) 234
PfeI GAWTC 2 cut(s) 253, 280
PpuMI RGGWCCY 1 cut(s) 260
Psp5II RGGWCCY 1 cut(s) 260
Psp6I CCWGG 1 cut(s) 61
PspGI CCWGG 1 cut(s) 61
PspN4I GGNNCC 3 cut(s) 169, 191, 261
PspPI GGNCC 2 cut(s) 168, 260
PspPPI RGGWCCY 1 cut(s) 260
RsaI GTAC 3 cut(s) 47, 154, 355
RsaNI GTAC 3 cut(s) 46, 153, 354
RseI CAYNNNNRTG 1 cut(s) 63
SalI GTCGAC 1 cut(s) 110
Sau3AI GATC 1 cut(s) 82
Sau96I GGNCC 2 cut(s) 168, 260
ScrFI CCNGG 1 cut(s) 63
SduI GDGCHC 1 cut(s) 70
SetI ASST 3 cut(s) 111, 143, 188
SfaNI GCATC 1 cut(s) 208
SinI GGWCC 2 cut(s) 168, 260
SmiMI CAYNNNNRTG 1 cut(s) 63
Sse9I AATT 1 cut(s) 52
StyD4I CCNGG 1 cut(s) 61
TaaI ACNGT 3 cut(s) 35, 50, 207
TaiI ACGT 2 cut(s) 111, 143
TaqI TCGA 1 cut(s) 111
TasI AATT 1 cut(s) 52
TatI WGTACW 1 cut(s) 45
TfiI GAWTC 2 cut(s) 253, 280
TscAI CASTG 3 cut(s) 75, 212, 231
TseFI GTSAC 2 cut(s) 8, 29
Tsp45I GTSAC 2 cut(s) 8, 29
TspGWI ACGGA 1 cut(s) 95
TspRI CASTG 3 cut(s) 75, 212, 231
VneI GTGCAC 1 cut(s) 66
VpaK11BI GGWCC 2 cut(s) 168, 260
XceI RCATGY 1 cut(s) 375
XmiI GTMKAC 1 cut(s) 111
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.