MD13G1246400.v1.1

Belongs to the glucose-6-phosphate 1-epimerase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0000789
Physical Location & Seq
Forward (+)
25941955 .. 25949286
7332 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1246400.v1.1.491

Sequence Viewer

Length: 1059 bp
ATGAGCAGCGACAAGGCTCCGAATCCAATCGCTGCATCTGCTGCAGTATCCCCCACAAATGCATCCAGTAATACTACCACTGGCGGTGGTGGCCCTGCTGCCGTTGCTAATGCTAACGTAAGCCTTCATCCCGGCGTTGAATCCTGTAAGGGCATCAATGGCCTTGACAAACTCGTCCTCCATGATCCACGAGGCAGTTCTGCTGAGGTGTATTTGTATGGGGCTCATGTAACTTCATGGAAGAATGATCATGGAGAGGAATTGCTATTTGTTAGCAGTAAGGCTATCTTTAAGCCTCCAAAAGCAATTCGTGGGGGAATCCCGTTATGCTTTCCGCAATTTTCAAATCTTGGTCCTCTTGAAGCACATGGATTTGCTAGAAACAGATTTTGGAGCATTGACTCTGATCCATCTCCGTTCCCAACAAATAGCCCCAATAAAGTTTTTATTGACTTGATCCTTAAGCCTACTGAAGAAGATATGAAGACCTGGCCTCACAGTTATGAGTTCCGGCTGCGGGTAGCTTTGGGACCTGGAGGAGATCTGACATTGATATCCCGCATCAGGAATACCAACACCGATGGGAAGCCATTCACATTCACATTTGCATATCACACTTACTTCGCTGTTTCAGATATCAGTGAAGTTCGCGTAGAAGGATTGGAAACCTTGGATTATCTTGACAACTTGCAGAGCAGGAAACGTTGCACTGAACAGGGCGATGCTATAACATTTGAGTCAGAGGTGGACAAGATATATCTGAGCACTCCTACAAAGATTGCAATTCTGGATCATGAGAAGAAGAGAACATTTGTAATACGCAAAGATGGTCTTCCAGATGCTGTTGTATGGAATCCCTGGGATAAGAAGGCGAAGGCTTTGACTGACTTTGGAGATGATGAATACAAGCATATGCTGTGTGTGGAGGCTGCTGCTATTGAGAAGCCTATAACATTGAAACCTGGAGAGGAGTGGAGAGGAAGGCAGGAGCTTTCAGCTGTTCCTTCCAGTTACTGCAGTGGGCAACTTGATCCTCGAAAGGTTCTTCAGGGAAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00010 ko01100 ko01110 ko01120 ko01130 map00010 map01100 map01110 map01120 map01130
Pfam Domains
Protein Families
Aldose_epim

Protein Analysis

353

Amino Acids

38.65

Weight (kDa)

6.16

Isoelectric Point (pI)

34.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldose_epim PF01263 57 - 331 2.9e-71 Aldose 1-epimerase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 173
AccII CGCG 1 cut(s) 651
AciI CCGC 4 cut(s) 84, 335, 517, 559
AclI AACGTT 1 cut(s) 703
AclWI GGATC 5 cut(s) 179, 401, 451, 798, 1025
AcuI CTGAAG 2 cut(s) 492, 1031
AfiI CCNNNNNNNGG 2 cut(s) 517, 564
AflII CTTAAG 1 cut(s) 461
AgsI TTSAA 4 cut(s) 140, 345, 362, 958
AjnI CCWGG 4 cut(s) 488, 532, 857, 961
AjuI GAANNNNNNNTTGG 2 cut(s) 373, 405
AluBI AGCT 4 cut(s) 524, 991, 998, 1056
AluI AGCT 4 cut(s) 524, 991, 998, 1056
Alw21I GWGCWC 1 cut(s) 767
AlwI GGATC 5 cut(s) 179, 401, 451, 798, 1025
AlwNI CAGNNNCTG 2 cut(s) 842, 1014
AoxI GGCC 3 cut(s) 91, 160, 491
ApeKI GCWGC 7 cut(s) 6, 32, 41, 98, 514, 929, 932
Asp700I GAANNNNTTC 1 cut(s) 590
AspS9I GGNCC 3 cut(s) 92, 353, 530
AsuC2I CCSGG 1 cut(s) 132
AvaII GGWCC 2 cut(s) 353, 530
BanII GRGCYC 1 cut(s) 226
BauI CACGAG 1 cut(s) 189
BbsI GAAGAC 2 cut(s) 491, 824
Bbv12I GWGCWC 1 cut(s) 767
BbvCI CCTCAGC 1 cut(s) 204
BbvI GCAGC 7 cut(s) 18, 19, 28, 85, 501, 916, 919
BccI CCATC 3 cut(s) 418, 575, 821
BceAI ACGGC 1 cut(s) 86
BciT130I CCWGG 4 cut(s) 490, 534, 859, 963
BciVI GTATCC 1 cut(s) 58
BclI TGATCA 1 cut(s) 247
BcnI CCSGG 1 cut(s) 132
BfaI CTAG 1 cut(s) 378
BfmI CTRYAG 2 cut(s) 42, 1015
BfrI CTTAAG 1 cut(s) 461
BfuI GTATCC 1 cut(s) 58
BglII AGATCT 1 cut(s) 541
BisI GCNGC 7 cut(s) 7, 33, 42, 99, 515, 930, 933
BlsI GCNGC 7 cut(s) 8, 34, 43, 100, 516, 931, 934
Bme1390I CCNGG 5 cut(s) 132, 490, 534, 859, 963
Bme18I GGWCC 2 cut(s) 353, 530
BmgT120I GGNCC 3 cut(s) 92, 353, 530
BmiI GGNNCC 2 cut(s) 18, 531
BmrFI CCNGG 5 cut(s) 132, 490, 534, 859, 963
BmsI GCATC 6 cut(s) 44, 71, 162, 570, 712, 829
BpiI GAAGAC 2 cut(s) 491, 824
BpmI CTGGAG 2 cut(s) 555, 984
Bpu10I CCTNAGC 1 cut(s) 204
BpuMI CCSGG 1 cut(s) 132
BsaJI CCNNGG 3 cut(s) 669, 857, 858
BsaXI ACNNNNNCTCC 2 cut(s) 162, 192
Bsc4I CCNNNNNNNGG 2 cut(s) 517, 564
Bse1I ACTGG 3 cut(s) 66, 85, 1008
BseBI CCWGG 4 cut(s) 490, 534, 859, 963
BseDI CCNNGG 3 cut(s) 669, 857, 858
BseGI GGATG 2 cut(s) 62, 127
BseLI CCNNNNNNNGG 2 cut(s) 517, 564
BseMII CTCAG 2 cut(s) 195, 752
BseNI ACTGG 3 cut(s) 66, 85, 1008
BseRI GAGGAG 2 cut(s) 552, 983
BseXI GCAGC 7 cut(s) 18, 19, 28, 85, 501, 916, 919
Bsh1236I CGCG 1 cut(s) 651
BshFI GGCC 3 cut(s) 93, 162, 493
BsiHKAI GWGCWC 1 cut(s) 767
BsiSI CCGG 2 cut(s) 132, 511
BslFI GGGAC 1 cut(s) 543
BslI CCNNNNNNNGG 2 cut(s) 517, 564
BsmFI GGGAC 1 cut(s) 543
BsnI GGCC 3 cut(s) 93, 162, 493
Bsp1286I GDGCHC 2 cut(s) 226, 767
Bsp143I GATC 7 cut(s) 184, 247, 406, 456, 541, 790, 1030
BspACI CCGC 4 cut(s) 84, 335, 517, 559
BspANI GGCC 3 cut(s) 93, 162, 493
BspCNI CTCAG 2 cut(s) 196, 753
BspFNI CGCG 1 cut(s) 651
BspHI TCATGA 1 cut(s) 793
BspLI GGNNCC 2 cut(s) 18, 531
BspMAI CTGCAG 2 cut(s) 46, 1019
BspPI GGATC 5 cut(s) 179, 401, 451, 798, 1025
BspTI CTTAAG 1 cut(s) 461
BsrI ACTGG 3 cut(s) 66, 85, 1008
BssECI CCNNGG 3 cut(s) 669, 857, 858
BssMI GATC 7 cut(s) 184, 247, 406, 456, 541, 790, 1030
BssSI CACGAG 1 cut(s) 189
BssT1I CCWWGG 1 cut(s) 669
Bst2BI CACGAG 1 cut(s) 189
Bst2UI CCWGG 4 cut(s) 490, 534, 859, 963
Bst4CI ACNGT 1 cut(s) 500
Bst6I CTCTTC 1 cut(s) 797
BstAFI CTTAAG 1 cut(s) 461
BstAPI GCANNNNNTGC 1 cut(s) 41
BstDEI CTNAG 2 cut(s) 204, 761
BstF5I GGATG 2 cut(s) 62, 127
BstFNI CGCG 1 cut(s) 651
BstKTI GATC 7 cut(s) 187, 250, 409, 459, 544, 793, 1033
BstMBI GATC 7 cut(s) 184, 247, 406, 456, 541, 790, 1030
BstMWI GCNNNNNNNGC 5 cut(s) 38, 41, 90, 104, 159
BstNI CCWGG 4 cut(s) 490, 534, 859, 963
BstSCI CCNGG 5 cut(s) 130, 488, 532, 857, 961
BstSFI CTRYAG 2 cut(s) 42, 1015
BstUI CGCG 1 cut(s) 651
BstV1I GCAGC 7 cut(s) 18, 19, 28, 85, 501, 916, 919
BstV2I GAAGAC 2 cut(s) 491, 824
BstX2I RGATCY 1 cut(s) 541
BstYI RGATCY 1 cut(s) 541
BsuI GTATCC 1 cut(s) 58
BsuRI GGCC 3 cut(s) 93, 162, 493
BtgZI GCGATG 1 cut(s) 735
BtsCI GGATG 2 cut(s) 62, 127
BtsI GCAGTG 1 cut(s) 1024
BtsIMutI CAGTG 4 cut(s) 78, 646, 708, 1024
CaiI CAGNNNCTG 2 cut(s) 842, 1014
CciI TCATGA 1 cut(s) 793
Cfr13I GGNCC 3 cut(s) 92, 353, 530
CviAII CATG 6 cut(s) 182, 227, 237, 251, 368, 794
DdeI CTNAG 2 cut(s) 204, 761
DpnI GATC 7 cut(s) 186, 249, 408, 458, 543, 792, 1032
DpnII GATC 7 cut(s) 184, 247, 406, 456, 541, 790, 1030
DrdI GACNNNNNNGTC 1 cut(s) 173
DseDI GACNNNNNNGTC 1 cut(s) 173
Eam1104I CTCTTC 1 cut(s) 797
EarI CTCTTC 1 cut(s) 797
Eco130I CCWWGG 1 cut(s) 669
Eco24I GRGCYC 1 cut(s) 226
Eco32I GATATC 2 cut(s) 555, 637
Eco47I GGWCC 2 cut(s) 353, 530
Eco57I CTGAAG 2 cut(s) 492, 1031
EcoO109I RGGNCCY 1 cut(s) 530
EcoRII CCWGG 4 cut(s) 488, 532, 857, 961
EcoRV GATATC 2 cut(s) 555, 637
EcoT14I CCWWGG 1 cut(s) 669
EcoT22I ATGCAT 1 cut(s) 64
EcoT38I GRGCYC 1 cut(s) 226
ErhI CCWWGG 1 cut(s) 669
FaeI CATG 6 cut(s) 185, 230, 240, 254, 371, 797
FalI AAGNNNNNCTT 4 cut(s) 272, 304, 816, 848
FaqI GGGAC 1 cut(s) 543
FatI CATG 6 cut(s) 181, 226, 236, 250, 367, 793
FauI CCCGC 2 cut(s) 510, 566
FauNDI CATATG 1 cut(s) 912
FbaI TGATCA 1 cut(s) 247
Fnu4HI GCNGC 7 cut(s) 7, 33, 42, 99, 515, 930, 933
FokI GGATG 2 cut(s) 49, 114
FriOI GRGCYC 1 cut(s) 226
Fsp4HI GCNGC 7 cut(s) 7, 33, 42, 99, 515, 930, 933
FspBI CTAG 1 cut(s) 378
GluI GCNGC 7 cut(s) 7, 33, 42, 99, 515, 930, 933
GsuI CTGGAG 2 cut(s) 555, 984
HaeIII GGCC 3 cut(s) 93, 162, 493
HapII CCGG 2 cut(s) 132, 511
Hin1II CATG 6 cut(s) 185, 230, 240, 254, 371, 797
HinfI GANTC 6 cut(s) 22, 140, 318, 401, 737, 853
HpaII CCGG 2 cut(s) 132, 511
Hpy166II GTNNAC 1 cut(s) 748
Hpy188I TCNGA 6 cut(s) 21, 406, 546, 634, 742, 762
Hpy188III TCNNGA 6 cut(s) 359, 565, 680, 788, 794, 836
Hpy8I GTNNAC 1 cut(s) 748
HpyAV CCTTC 6 cut(s) 134, 650, 862, 868, 975, 1014
HpyCH4III ACNGT 1 cut(s) 500
HpyCH4IV ACGT 2 cut(s) 117, 703
HpyCH4V TGCA 8 cut(s) 35, 44, 62, 608, 691, 708, 782, 1017
HpyF10VI GCNNNNNNNGC 5 cut(s) 38, 41, 90, 104, 159
HpyF3I CTNAG 2 cut(s) 204, 761
HpySE526I ACGT 2 cut(s) 117, 703
Hsp92II CATG 6 cut(s) 185, 230, 240, 254, 371, 797
Ksp22I TGATCA 1 cut(s) 247
Kzo9I GATC 7 cut(s) 184, 247, 406, 456, 541, 790, 1030
LmnI GCTCC 3 cut(s) 22, 393, 988
Lsp1109I GCAGC 7 cut(s) 18, 19, 28, 85, 501, 916, 919
LweI GCATC 6 cut(s) 44, 71, 162, 570, 712, 829
MaeI CTAG 1 cut(s) 378
MaeII ACGT 2 cut(s) 117, 703
MaeIII GTNAC 2 cut(s) 229, 1010
MalI GATC 7 cut(s) 186, 249, 408, 458, 543, 792, 1032
MboI GATC 7 cut(s) 184, 247, 406, 456, 541, 790, 1030
MboII GAAGA 8 cut(s) 253, 485, 488, 496, 811, 814, 824, 1037
MflI RGATCY 1 cut(s) 541
MhlI GDGCHC 2 cut(s) 226, 767
MluCI AATT 4 cut(s) 260, 306, 338, 783
MlyI GAGTC 2 cut(s) 395, 746
Mph1103I ATGCAT 1 cut(s) 64
MroXI GAANNNNTTC 1 cut(s) 590
MseI TTAA 2 cut(s) 291, 462
MslI CAYNNNNRTG 1 cut(s) 501
MspA1I CMGCKG 1 cut(s) 998
MspCI CTTAAG 1 cut(s) 461
MspI CCGG 2 cut(s) 132, 511
MspR9I CCNGG 5 cut(s) 132, 490, 534, 859, 963
MvaI CCWGG 4 cut(s) 490, 534, 859, 963
MvnI CGCG 1 cut(s) 651
MwoI GCNNNNNNNGC 5 cut(s) 38, 41, 90, 104, 159
NciI CCSGG 1 cut(s) 132
NdeI CATATG 1 cut(s) 912
NdeII GATC 7 cut(s) 184, 247, 406, 456, 541, 790, 1030
NlaIII CATG 6 cut(s) 185, 230, 240, 254, 371, 797
NlaIV GGNNCC 2 cut(s) 18, 531
NsiI ATGCAT 1 cut(s) 64
PagI TCATGA 1 cut(s) 793
PasI CCCWGGG 1 cut(s) 858
PdmI GAANNNNTTC 1 cut(s) 590
PfeI GAWTC 4 cut(s) 22, 140, 318, 853
PkrI GCNGC 7 cut(s) 8, 34, 43, 100, 516, 931, 934
PleI GAGTC 2 cut(s) 395, 745
PpsI GAGTC 2 cut(s) 395, 745
PpuMI RGGWCCY 1 cut(s) 530
Psp1406I AACGTT 1 cut(s) 703
Psp5II RGGWCCY 1 cut(s) 530
Psp6I CCWGG 4 cut(s) 488, 532, 857, 961
PspGI CCWGG 4 cut(s) 488, 532, 857, 961
PspN4I GGNNCC 2 cut(s) 18, 531
PspPI GGNCC 3 cut(s) 92, 353, 530
PspPPI RGGWCCY 1 cut(s) 530
PstI CTGCAG 2 cut(s) 46, 1019
PstNI CAGNNNCTG 2 cut(s) 842, 1014
PsuI RGATCY 1 cut(s) 541
PvuII CAGCTG 1 cut(s) 998
RseI CAYNNNNRTG 1 cut(s) 501
SaqAI TTAA 2 cut(s) 291, 462
SatI GCNGC 7 cut(s) 7, 33, 42, 99, 515, 930, 933
Sau3AI GATC 7 cut(s) 184, 247, 406, 456, 541, 790, 1030
Sau96I GGNCC 3 cut(s) 92, 353, 530
SchI GAGTC 2 cut(s) 395, 746
ScrFI CCNGG 5 cut(s) 132, 490, 534, 859, 963
SduI GDGCHC 2 cut(s) 226, 767
SfaNI GCATC 6 cut(s) 44, 71, 162, 570, 712, 829
SfcI CTRYAG 2 cut(s) 42, 1015
SinI GGWCC 2 cut(s) 353, 530
SmiMI CAYNNNNRTG 1 cut(s) 501
SmlI CTYRAG 1 cut(s) 461
SmoI CTYRAG 1 cut(s) 461
Sse9I AATT 4 cut(s) 260, 306, 338, 783
SsiI CCGC 4 cut(s) 84, 335, 517, 559
SspMI CTAG 1 cut(s) 378
StyD4I CCNGG 5 cut(s) 130, 488, 532, 857, 961
StyI CCWWGG 1 cut(s) 669
TaaI ACNGT 1 cut(s) 500
TaiI ACGT 2 cut(s) 120, 706
TaqI TCGA 1 cut(s) 1036
TasI AATT 4 cut(s) 260, 306, 338, 783
TfiI GAWTC 4 cut(s) 22, 140, 318, 853
Tru1I TTAA 2 cut(s) 291, 462
Tru9I TTAA 2 cut(s) 291, 462
TscAI CASTG 4 cut(s) 85, 646, 715, 1024
TseI GCWGC 7 cut(s) 6, 32, 41, 98, 514, 929, 932
TspDTI ATGAA 4 cut(s) 116, 225, 497, 915
TspGWI ACGGA 1 cut(s) 405
TspRI CASTG 4 cut(s) 85, 646, 715, 1024
Vha464I CTTAAG 1 cut(s) 461
VpaK11BI GGWCC 2 cut(s) 353, 530
XmnI GAANNNNTTC 1 cut(s) 590
XspI CTAG 1 cut(s) 378
Zsp2I ATGCAT 1 cut(s) 64
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.