pycom16g21500

Belongs to the glucose-6-phosphate 1-epimerase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0000789
Physical Location & Seq
Forward (+)
20068185 .. 20073786
5602 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g21500.3

Sequence Viewer

Length: 1047 bp
ATGAGCAGCGACAAGGCTCCGAATCCAACCGCTGCTTCTGCTGCAGCGGCCATTAATGCATCGATTGATACTAGCAGTGGCGCTGGCGCTGTTGCTAATGCTAACGCAAGCCTTCATCCCGGCGTTGAATCCAGTAAGGGCGTCAATGGCCTCGACAAGCTCGTCCTCCATGATCCACGAGGCAGTTCCGCGGAGGTGTATCTGTACGGGGCTCATGTAACTTCCTGGAAGAATGATCATGGAGAGGAATTGCTATTTGTTAGCAGTAAGGCTATATTTAAGCCTCCAAAAGCAATTCGTGGGGGAATCCCATTATGCTTTCCGCAATTTTCCAATCTTGGTTCTCTTGATGCTCATGGATTTGCTAGAAACAGAGTTTGGAGCATTGACACCGATCGATCTCCTTTCCCAACAAATAGCCCCAATAAAGTTTCTGTTGACTTGATCCTTAAGCCTACCGAGGAAGATATGAAGACCTGGCCTCACAGTTATGAGTTCCGACTGCGGGTAGCTTTGGGACCCGGAGGAGATCTGACGTTGATATCTCGCATCAGGAATACTAACACCGATGGGAAGCCGTTCACATTCACATTCGCATATCACACTTACTTCTCTGTTTCAGATATCAGCGAAGTTCGTGTAGAAGGATTGGAAACATTGGATTATCTTGACAACACTCAGAACAGGGAACGTTGCACTGAACAAGGCGATGCTATAACATTTGAGTCAGAGGTGGACAAGATATATCTCAGCACTCCCACAAAGATTGCCATTCTGGATCATGAGAAGAAGAGAACATTTGTAATACGCAAAGATGGTCTTCCAGATGCCGTTGTATGGAATCCCTGGGATAAGAAGGCGAAGGCTTTGACTGATTTTGGAGATGATGAATACAAGCATATGCTGTGTGTGGAGGCCGCTGCTATTGAGAAACCTATAACATTGAAACCTGGAGAGGAGTGGAGAGGAAGGCAGGAGCTTTCAGCTGTTCCTTCCAGTTACTGCAGCGGACAACTCGATCCTCGAAAGGTTCTCCAGGGAATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00010 ko01100 ko01110 ko01120 ko01130 map00010 map01100 map01110 map01120 map01130
Pfam Domains
Protein Families
Aldose_epim

Protein Analysis

349

Amino Acids

38.23

Weight (kDa)

5.89

Isoelectric Point (pI)

26.68

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 161
AccII CGCG 1 cut(s) 191
AciI CCGC 8 cut(s) 30, 47, 189, 191, 323, 505, 918, 1008
AclI AACGTT 1 cut(s) 691
AclWI GGATC 4 cut(s) 167, 439, 786, 1013
AcoI YGGCCR 1 cut(s) 48
AcyI GRCGYC 1 cut(s) 141
AfaI GTAC 1 cut(s) 206
AfiI CCNNNNNNNGG 2 cut(s) 505, 837
AflII CTTAAG 1 cut(s) 449
AgsI TTSAA 2 cut(s) 128, 946
AjnI CCWGG 5 cut(s) 224, 476, 845, 949, 1035
AjuI GAANNNNNNNTTGG 4 cut(s) 19, 51, 361, 393
AluBI AGCT 4 cut(s) 160, 512, 979, 986
AluI AGCT 4 cut(s) 160, 512, 979, 986
AlwI GGATC 4 cut(s) 167, 439, 786, 1013
AlwNI CAGNNNCTG 1 cut(s) 1002
AoxI GGCC 4 cut(s) 48, 148, 479, 915
ApeKI GCWGC 6 cut(s) 6, 32, 41, 44, 920, 1005
AseI ATTAAT 1 cut(s) 54
Asp700I GAANNNNTTC 1 cut(s) 578
AspLEI GCGC 2 cut(s) 83, 89
AspS9I GGNCC 1 cut(s) 518
AsuC2I CCSGG 2 cut(s) 120, 522
AvaII GGWCC 1 cut(s) 518
BanII GRGCYC 1 cut(s) 214
BauI CACGAG 1 cut(s) 177
BbsI GAAGAC 2 cut(s) 479, 812
BbvI GCAGC 6 cut(s) 18, 19, 28, 56, 907, 1017
BccI CCATC 2 cut(s) 563, 809
BceAI ACGGC 2 cut(s) 562, 815
BciT130I CCWGG 5 cut(s) 226, 478, 847, 951, 1037
BclI TGATCA 1 cut(s) 235
BcnI CCSGG 2 cut(s) 120, 522
BfaI CTAG 2 cut(s) 72, 366
BfmI CTRYAG 2 cut(s) 42, 1003
BfoI RGCGCY 2 cut(s) 84, 90
BfrI CTTAAG 1 cut(s) 449
BglII AGATCT 1 cut(s) 529
BisI GCNGC 8 cut(s) 7, 33, 42, 45, 48, 918, 921, 1006
BlsI GCNGC 8 cut(s) 8, 34, 43, 46, 49, 919, 922, 1007
Bme1390I CCNGG 7 cut(s) 120, 226, 478, 522, 847, 951, 1037
Bme18I GGWCC 1 cut(s) 518
BmgT120I GGNCC 1 cut(s) 518
BmiI GGNNCC 3 cut(s) 18, 519, 520
BmrFI CCNGG 7 cut(s) 120, 226, 478, 522, 847, 951, 1037
BmsI GCATC 5 cut(s) 68, 340, 558, 700, 817
BpiI GAAGAC 2 cut(s) 479, 812
BpmI CTGGAG 2 cut(s) 972, 1019
BpuMI CCSGG 2 cut(s) 120, 522
Bsa29I ATCGAT 2 cut(s) 62, 397
BsaHI GRCGYC 1 cut(s) 141
BsaJI CCNNGG 5 cut(s) 189, 459, 845, 846, 1036
Bsc4I CCNNNNNNNGG 2 cut(s) 505, 837
Bse1I ACTGG 2 cut(s) 132, 996
BseBI CCWGG 5 cut(s) 226, 478, 847, 951, 1037
BseCI ATCGAT 2 cut(s) 62, 397
BseDI CCNNGG 5 cut(s) 189, 459, 845, 846, 1036
BseGI GGATG 1 cut(s) 115
BseLI CCNNNNNNNGG 2 cut(s) 505, 837
BseMII CTCAG 2 cut(s) 692, 763
BseNI ACTGG 2 cut(s) 132, 996
BseRI GAGGAG 2 cut(s) 540, 971
BseXI GCAGC 6 cut(s) 18, 19, 28, 56, 907, 1017
Bsh1236I CGCG 1 cut(s) 191
Bsh1285I CGRYCG 1 cut(s) 397
BshFI GGCC 4 cut(s) 50, 150, 481, 917
BshVI ATCGAT 2 cut(s) 62, 397
BsiEI CGRYCG 1 cut(s) 397
BsiSI CCGG 2 cut(s) 120, 522
BslFI GGGAC 1 cut(s) 531
BslI CCNNNNNNNGG 2 cut(s) 505, 837
BsmFI GGGAC 1 cut(s) 531
BsnI GGCC 4 cut(s) 50, 150, 481, 917
Bsp1286I GDGCHC 1 cut(s) 214
Bsp143I GATC 8 cut(s) 172, 235, 394, 398, 444, 529, 778, 1018
BspACI CCGC 8 cut(s) 30, 47, 189, 191, 323, 505, 918, 1008
BspANI GGCC 4 cut(s) 50, 150, 481, 917
BspCNI CTCAG 2 cut(s) 691, 762
BspDI ATCGAT 2 cut(s) 62, 397
BspFNI CGCG 1 cut(s) 191
BspHI TCATGA 1 cut(s) 781
BspLI GGNNCC 3 cut(s) 18, 519, 520
BspMAI CTGCAG 2 cut(s) 46, 1007
BspPI GGATC 4 cut(s) 167, 439, 786, 1013
BspTI CTTAAG 1 cut(s) 449
BsrI ACTGG 2 cut(s) 132, 996
BssECI CCNNGG 5 cut(s) 189, 459, 845, 846, 1036
BssMI GATC 8 cut(s) 172, 235, 394, 398, 444, 529, 778, 1018
BssNI GRCGYC 1 cut(s) 141
BssSI CACGAG 1 cut(s) 177
Bst2BI CACGAG 1 cut(s) 177
Bst2UI CCWGG 5 cut(s) 226, 478, 847, 951, 1037
Bst4CI ACNGT 1 cut(s) 488
Bst6I CTCTTC 1 cut(s) 785
BstACI GRCGYC 1 cut(s) 141
BstAFI CTTAAG 1 cut(s) 449
BstC8I GCNNGC 2 cut(s) 85, 109
BstDEI CTNAG 2 cut(s) 678, 749
BstDSI CCRYGG 1 cut(s) 189
BstF5I GGATG 1 cut(s) 115
BstFNI CGCG 1 cut(s) 191
BstH2I RGCGCY 2 cut(s) 84, 90
BstHHI GCGC 2 cut(s) 83, 89
BstKTI GATC 8 cut(s) 175, 238, 397, 401, 447, 532, 781, 1021
BstMBI GATC 8 cut(s) 172, 235, 394, 398, 444, 529, 778, 1018
BstMCI CGRYCG 1 cut(s) 397
BstMWI GCNNNNNNNGC 5 cut(s) 38, 41, 47, 56, 147
BstNI CCWGG 5 cut(s) 226, 478, 847, 951, 1037
BstSCI CCNGG 7 cut(s) 118, 224, 476, 520, 845, 949, 1035
BstSFI CTRYAG 2 cut(s) 42, 1003
BstUI CGCG 1 cut(s) 191
BstV1I GCAGC 6 cut(s) 18, 19, 28, 56, 907, 1017
BstV2I GAAGAC 2 cut(s) 479, 812
BstX2I RGATCY 1 cut(s) 529
BstYI RGATCY 1 cut(s) 529
Bsu15I ATCGAT 2 cut(s) 62, 397
BsuRI GGCC 4 cut(s) 50, 150, 481, 917
BsuTUI ATCGAT 2 cut(s) 62, 397
BtgI CCRYGG 1 cut(s) 189
BtgZI GCGATG 1 cut(s) 723
BtsCI GGATG 1 cut(s) 115
BtsI GCAGTG 1 cut(s) 82
BtsIMutI CAGTG 2 cut(s) 82, 696
Cac8I GCNNGC 2 cut(s) 85, 109
CaiI CAGNNNCTG 1 cut(s) 1002
CciI TCATGA 1 cut(s) 781
CfoI GCGC 2 cut(s) 83, 89
Cfr13I GGNCC 1 cut(s) 518
Cfr42I CCGCGG 1 cut(s) 192
ClaI ATCGAT 2 cut(s) 62, 397
CseI GACGC 1 cut(s) 130
Csp6I GTAC 1 cut(s) 205
CspCI CAANNNNNGTGG 2 cut(s) 748, 783
CviAII CATG 5 cut(s) 170, 215, 239, 356, 782
CviQI GTAC 1 cut(s) 205
DdeI CTNAG 2 cut(s) 678, 749
DpnI GATC 8 cut(s) 174, 237, 396, 400, 446, 531, 780, 1020
DpnII GATC 8 cut(s) 172, 235, 394, 398, 444, 529, 778, 1018
DrdI GACNNNNNNGTC 1 cut(s) 161
DseDI GACNNNNNNGTC 1 cut(s) 161
EaeI YGGCCR 1 cut(s) 48
Eam1104I CTCTTC 1 cut(s) 785
EarI CTCTTC 1 cut(s) 785
Eco24I GRGCYC 1 cut(s) 214
Eco32I GATATC 2 cut(s) 543, 625
Eco47I GGWCC 1 cut(s) 518
EcoO109I RGGNCCY 1 cut(s) 518
EcoRII CCWGG 5 cut(s) 224, 476, 845, 949, 1035
EcoRV GATATC 2 cut(s) 543, 625
EcoT22I ATGCAT 1 cut(s) 61
EcoT38I GRGCYC 1 cut(s) 214
FaeI CATG 5 cut(s) 173, 218, 242, 359, 785
FalI AAGNNNNNCTT 2 cut(s) 804, 836
FaqI GGGAC 1 cut(s) 531
FatI CATG 5 cut(s) 169, 214, 238, 355, 781
FauI CCCGC 1 cut(s) 498
FauNDI CATATG 1 cut(s) 900
FbaI TGATCA 1 cut(s) 235
Fnu4HI GCNGC 8 cut(s) 7, 33, 42, 45, 48, 918, 921, 1006
FokI GGATG 1 cut(s) 102
FriOI GRGCYC 1 cut(s) 214
Fsp4HI GCNGC 8 cut(s) 7, 33, 42, 45, 48, 918, 921, 1006
FspBI CTAG 2 cut(s) 72, 366
GlaI GCGC 2 cut(s) 82, 88
GluI GCNGC 8 cut(s) 7, 33, 42, 45, 48, 918, 921, 1006
GsuI CTGGAG 2 cut(s) 972, 1019
HaeII RGCGCY 2 cut(s) 84, 90
HaeIII GGCC 4 cut(s) 50, 150, 481, 917
HapII CCGG 2 cut(s) 120, 522
HgaI GACGC 1 cut(s) 130
HhaI GCGC 2 cut(s) 83, 89
Hin1I GRCGYC 1 cut(s) 141
Hin1II CATG 5 cut(s) 173, 218, 242, 359, 785
Hin6I GCGC 2 cut(s) 81, 87
HinP1I GCGC 2 cut(s) 81, 87
HincII GTYRAC 1 cut(s) 439
HindII GTYRAC 1 cut(s) 439
HinfI GANTC 6 cut(s) 22, 128, 306, 725, 841, 1041
HpaII CCGG 2 cut(s) 120, 522
Hpy166II GTNNAC 3 cut(s) 439, 582, 736
Hpy188I TCNGA 7 cut(s) 21, 500, 534, 622, 681, 730, 1046
Hpy188III TCNNGA 6 cut(s) 347, 553, 668, 776, 782, 824
Hpy8I GTNNAC 3 cut(s) 439, 582, 736
HpyAV CCTTC 6 cut(s) 122, 638, 850, 856, 963, 1002
HpyCH4III ACNGT 1 cut(s) 488
HpyCH4IV ACGT 2 cut(s) 536, 691
HpyCH4V TGCA 4 cut(s) 44, 59, 696, 1005
HpyF10VI GCNNNNNNNGC 5 cut(s) 38, 41, 47, 56, 147
HpyF3I CTNAG 2 cut(s) 678, 749
HpySE526I ACGT 2 cut(s) 536, 691
Hsp92I GRCGYC 1 cut(s) 141
Hsp92II CATG 5 cut(s) 173, 218, 242, 359, 785
HspAI GCGC 2 cut(s) 81, 87
KflI GGGWCCC 1 cut(s) 518
Ksp22I TGATCA 1 cut(s) 235
KspI CCGCGG 1 cut(s) 192
Kzo9I GATC 8 cut(s) 172, 235, 394, 398, 444, 529, 778, 1018
LmnI GCTCC 3 cut(s) 22, 381, 976
Lsp1109I GCAGC 6 cut(s) 18, 19, 28, 56, 907, 1017
LweI GCATC 5 cut(s) 68, 340, 558, 700, 817
MaeI CTAG 2 cut(s) 72, 366
MaeII ACGT 2 cut(s) 536, 691
MaeIII GTNAC 2 cut(s) 217, 998
MalI GATC 8 cut(s) 174, 237, 396, 400, 446, 531, 780, 1020
MboI GATC 8 cut(s) 172, 235, 394, 398, 444, 529, 778, 1018
MboII GAAGA 6 cut(s) 241, 476, 484, 799, 802, 812
MflI RGATCY 1 cut(s) 529
MhlI GDGCHC 1 cut(s) 214
MluCI AATT 3 cut(s) 248, 294, 326
MlyI GAGTC 1 cut(s) 734
MmeI TCCRAC 2 cut(s) 50, 523
Mph1103I ATGCAT 1 cut(s) 61
MroXI GAANNNNTTC 1 cut(s) 578
MseI TTAA 3 cut(s) 54, 279, 450
MslI CAYNNNNRTG 1 cut(s) 489
MspA1I CMGCKG 6 cut(s) 32, 47, 191, 920, 986, 1008
MspCI CTTAAG 1 cut(s) 449
MspI CCGG 2 cut(s) 120, 522
MspR9I CCNGG 7 cut(s) 120, 226, 478, 522, 847, 951, 1037
MvaI CCWGG 5 cut(s) 226, 478, 847, 951, 1037
MvnI CGCG 1 cut(s) 191
MwoI GCNNNNNNNGC 5 cut(s) 38, 41, 47, 56, 147
NciI CCSGG 2 cut(s) 120, 522
NdeI CATATG 1 cut(s) 900
NdeII GATC 8 cut(s) 172, 235, 394, 398, 444, 529, 778, 1018
NlaIII CATG 5 cut(s) 173, 218, 242, 359, 785
NlaIV GGNNCC 3 cut(s) 18, 519, 520
NsiI ATGCAT 1 cut(s) 61
PagI TCATGA 1 cut(s) 781
PasI CCCWGGG 1 cut(s) 846
PcsI WCGNNNNNNNCGW 1 cut(s) 159
PdmI GAANNNNTTC 1 cut(s) 578
PfeI GAWTC 5 cut(s) 22, 128, 306, 841, 1041
PfoI TCCNGGA 1 cut(s) 224
PkrI GCNGC 8 cut(s) 8, 34, 43, 46, 49, 919, 922, 1007
Ple19I CGATCG 1 cut(s) 397
PleI GAGTC 1 cut(s) 733
PpsI GAGTC 1 cut(s) 733
PpuMI RGGWCCY 1 cut(s) 518
PshBI ATTAAT 1 cut(s) 54
Psp1406I AACGTT 1 cut(s) 691
Psp5II RGGWCCY 1 cut(s) 518
Psp6I CCWGG 5 cut(s) 224, 476, 845, 949, 1035
PspGI CCWGG 5 cut(s) 224, 476, 845, 949, 1035
PspN4I GGNNCC 3 cut(s) 18, 519, 520
PspPI GGNCC 1 cut(s) 518
PspPPI RGGWCCY 1 cut(s) 518
PstI CTGCAG 2 cut(s) 46, 1007
PstNI CAGNNNCTG 1 cut(s) 1002
PsuI RGATCY 1 cut(s) 529
PvuI CGATCG 1 cut(s) 397
PvuII CAGCTG 1 cut(s) 986
RsaI GTAC 1 cut(s) 206
RsaNI GTAC 1 cut(s) 205
RseI CAYNNNNRTG 1 cut(s) 489
SacII CCGCGG 1 cut(s) 192
SaqAI TTAA 3 cut(s) 54, 279, 450
SatI GCNGC 8 cut(s) 7, 33, 42, 45, 48, 918, 921, 1006
Sau3AI GATC 8 cut(s) 172, 235, 394, 398, 444, 529, 778, 1018
Sau96I GGNCC 1 cut(s) 518
SchI GAGTC 1 cut(s) 734
ScrFI CCNGG 7 cut(s) 120, 226, 478, 522, 847, 951, 1037
SduI GDGCHC 1 cut(s) 214
SfaNI GCATC 5 cut(s) 68, 340, 558, 700, 817
SfcI CTRYAG 2 cut(s) 42, 1003
Sfr303I CCGCGG 1 cut(s) 192
SgrBI CCGCGG 1 cut(s) 192
SinI GGWCC 1 cut(s) 518
SmiMI CAYNNNNRTG 1 cut(s) 489
SmlI CTYRAG 1 cut(s) 449
SmoI CTYRAG 1 cut(s) 449
Sse9I AATT 3 cut(s) 248, 294, 326
SsiI CCGC 8 cut(s) 30, 47, 189, 191, 323, 505, 918, 1008
SspMI CTAG 2 cut(s) 72, 366
StyD4I CCNGG 7 cut(s) 118, 224, 476, 520, 845, 949, 1035
TaaI ACNGT 1 cut(s) 488
TaiI ACGT 2 cut(s) 539, 694
TaqI TCGA 5 cut(s) 62, 153, 397, 1017, 1024
TasI AATT 3 cut(s) 248, 294, 326
TauI GCSGC 2 cut(s) 50, 920
TfiI GAWTC 5 cut(s) 22, 128, 306, 841, 1041
Tru1I TTAA 3 cut(s) 54, 279, 450
Tru9I TTAA 3 cut(s) 54, 279, 450
TscAI CASTG 2 cut(s) 82, 703
TseI GCWGC 6 cut(s) 6, 32, 41, 44, 920, 1005
TspDTI ATGAA 3 cut(s) 104, 485, 903
TspRI CASTG 2 cut(s) 82, 703
Vha464I CTTAAG 1 cut(s) 449
VpaK11BI GGWCC 1 cut(s) 518
VspI ATTAAT 1 cut(s) 54
XmnI GAANNNNTTC 1 cut(s) 578
XspI CTAG 2 cut(s) 72, 366
Zsp2I ATGCAT 1 cut(s) 61
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.