MD16G1253800.v1.1

Belongs to the glucose-6-phosphate 1-epimerase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0000789
Physical Location & Seq
Forward (+)
28882874 .. 28890103
7230 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1253800.v1.1.491

Sequence Viewer

Length: 1047 bp
ATGAGCAGCGACAAGGCTCCGAATCCAACCGCTGCTTCTGCTGCATCGGCCATTAATGCATCGATTGATACTAGCAGTGGCGCTCGCGCTGTTGCTAATGCTAACGCAAGCCTTCATCCCGGCGTTGAATCCAGCAAGGGCGTCAATGGCCTCGACAAGCTCGTCCTCCATGATCCACGAGGCAGTTCCGCGGAGGTGTATCTGTACGGGGCTCATGTAACTTCATGGAAGAATGATCATGGAGAGGAATTGCTATTTGTTAGCAGTAAGGCTATATTTAAGCCCCCAAAAGCAATTCGTGGGGGAATCCCATTATGCTTTCCGCAATTTTCCAATCTTGGTTCTCTTGATGCACATGGATTTGCTAGAAACAGAGTTTGGAGCATCGACACCGATCCATCTCCTTTCCCAACAAATAGTCCCAATAAAGTTTCTGTTGACTTGATCCTTAAGCCTACTGAGGAAGATATGAAGACCTGGCCTCACAGTTATGAGTTCCGACTGCGGGTAGCTTTGGGACCTGGAGGAGATCTGACGTTGATATCTCGCATCAGGAATACTAACACCGATGGGAAGCCATTCACATTCACATTTGCATATCACACTTACTTCTCTGTTTCAGATATCAGCGAAGTTCGTGTAGAAGGATTGGAAACATTGGATTATCTTGACAACATGCAGAACAGGAAACGTTGCACTGAACAAGGCGATGCTATAACATTTGAGTCAGAGGTGGACAAGATATATCTCAGCACTCCTACAAAGATTGCCATTCTGGATCATGAGAAGAAGAGAACATTTGTAATACGCAAAGATGGTCTTCCAGATGCCGTTGTATGGAATCCCTGGGATAAGAAGGCGAAGGCTTTGACTGATTTTGGAGACGATGAATACAAGCATATGCTGTGTGTGGAGGCCGCTGCTATTGAGAAACCAATAACATTGAAACCTGGAGAGGAGTGGAGAGGAAGGCAGGAGCTTTCAGCTGTTCCTTCCAGTTACTGCAGCGGACAACTAGATCCTCGAAAGGTTCTCCAGGGAATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00010 ko01100 ko01110 ko01120 ko01130 map00010 map01100 map01110 map01120 map01130
Pfam Domains
Protein Families
Aldose_epim

Protein Analysis

349

Amino Acids

38.31

Weight (kDa)

6.16

Isoelectric Point (pI)

27.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldose_epim PF01263 53 - 327 2.2e-71 Aldose 1-epimerase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 161
AccII CGCG 2 cut(s) 87, 191
AciI CCGC 7 cut(s) 30, 189, 191, 323, 505, 918, 1008
AclI AACGTT 1 cut(s) 691
AclWI GGATC 5 cut(s) 167, 389, 439, 786, 1013
AcoI YGGCCR 1 cut(s) 48
AcyI GRCGYC 1 cut(s) 141
AfaI GTAC 1 cut(s) 206
AfiI CCNNNNNNNGG 2 cut(s) 505, 837
AflII CTTAAG 1 cut(s) 449
AgsI TTSAA 2 cut(s) 128, 946
AjnI CCWGG 5 cut(s) 476, 520, 845, 949, 1035
AjuI GAANNNNNNNTTGG 4 cut(s) 19, 51, 361, 393
AluBI AGCT 4 cut(s) 160, 512, 979, 986
AluI AGCT 4 cut(s) 160, 512, 979, 986
Alw26I GTCTC 1 cut(s) 876
AlwI GGATC 5 cut(s) 167, 389, 439, 786, 1013
AlwNI CAGNNNCTG 1 cut(s) 1002
AoxI GGCC 4 cut(s) 48, 148, 479, 915
ApeKI GCWGC 5 cut(s) 6, 32, 41, 920, 1005
AseI ATTAAT 1 cut(s) 54
Asp700I GAANNNNTTC 1 cut(s) 578
AspLEI GCGC 2 cut(s) 83, 89
AspS9I GGNCC 1 cut(s) 518
AsuC2I CCSGG 1 cut(s) 120
AvaII GGWCC 1 cut(s) 518
BanII GRGCYC 1 cut(s) 214
BauI CACGAG 1 cut(s) 177
BbsI GAAGAC 2 cut(s) 479, 812
BbvI GCAGC 5 cut(s) 18, 19, 28, 907, 1017
BccI CCATC 3 cut(s) 406, 563, 809
BceAI ACGGC 1 cut(s) 815
BciT130I CCWGG 5 cut(s) 478, 522, 847, 951, 1037
BclI TGATCA 1 cut(s) 235
BcnI CCSGG 1 cut(s) 120
BcoDI GTCTC 1 cut(s) 876
BfaI CTAG 3 cut(s) 72, 366, 1016
BfmI CTRYAG 1 cut(s) 1003
BfoI RGCGCY 1 cut(s) 84
BfrI CTTAAG 1 cut(s) 449
BglII AGATCT 1 cut(s) 529
BisI GCNGC 6 cut(s) 7, 33, 42, 918, 921, 1006
BlsI GCNGC 6 cut(s) 8, 34, 43, 919, 922, 1007
Bme1390I CCNGG 6 cut(s) 120, 478, 522, 847, 951, 1037
Bme18I GGWCC 1 cut(s) 518
BmgT120I GGNCC 1 cut(s) 518
BmiI GGNNCC 2 cut(s) 18, 519
BmrFI CCNGG 6 cut(s) 120, 478, 522, 847, 951, 1037
BmsI GCATC 7 cut(s) 53, 68, 340, 393, 558, 700, 817
BpiI GAAGAC 2 cut(s) 479, 812
BpmI CTGGAG 3 cut(s) 543, 972, 1019
BpuMI CCSGG 1 cut(s) 120
Bsa29I ATCGAT 1 cut(s) 62
BsaHI GRCGYC 1 cut(s) 141
BsaJI CCNNGG 4 cut(s) 189, 845, 846, 1036
Bsc4I CCNNNNNNNGG 2 cut(s) 505, 837
Bse1I ACTGG 1 cut(s) 996
BseBI CCWGG 5 cut(s) 478, 522, 847, 951, 1037
BseCI ATCGAT 1 cut(s) 62
BseDI CCNNGG 4 cut(s) 189, 845, 846, 1036
BseGI GGATG 1 cut(s) 115
BseLI CCNNNNNNNGG 2 cut(s) 505, 837
BseMII CTCAG 2 cut(s) 450, 763
BseNI ACTGG 1 cut(s) 996
BseRI GAGGAG 2 cut(s) 540, 971
BseXI GCAGC 5 cut(s) 18, 19, 28, 907, 1017
Bsh1236I CGCG 2 cut(s) 87, 191
BshFI GGCC 4 cut(s) 50, 150, 481, 917
BshVI ATCGAT 1 cut(s) 62
BsiSI CCGG 1 cut(s) 120
BslFI GGGAC 2 cut(s) 405, 531
BslI CCNNNNNNNGG 2 cut(s) 505, 837
BsmAI GTCTC 1 cut(s) 876
BsmBI CGTCTC 1 cut(s) 876
BsmFI GGGAC 2 cut(s) 405, 531
BsnI GGCC 4 cut(s) 50, 150, 481, 917
Bsp1286I GDGCHC 1 cut(s) 214
Bsp143I GATC 7 cut(s) 172, 235, 394, 444, 529, 778, 1018
BspACI CCGC 7 cut(s) 30, 189, 191, 323, 505, 918, 1008
BspANI GGCC 4 cut(s) 50, 150, 481, 917
BspCNI CTCAG 2 cut(s) 451, 762
BspDI ATCGAT 1 cut(s) 62
BspFNI CGCG 2 cut(s) 87, 191
BspHI TCATGA 1 cut(s) 781
BspLI GGNNCC 2 cut(s) 18, 519
BspMAI CTGCAG 1 cut(s) 1007
BspPI GGATC 5 cut(s) 167, 389, 439, 786, 1013
BspTI CTTAAG 1 cut(s) 449
BsrI ACTGG 1 cut(s) 996
BssECI CCNNGG 4 cut(s) 189, 845, 846, 1036
BssMI GATC 7 cut(s) 172, 235, 394, 444, 529, 778, 1018
BssNI GRCGYC 1 cut(s) 141
BssSI CACGAG 1 cut(s) 177
Bst2BI CACGAG 1 cut(s) 177
Bst2UI CCWGG 5 cut(s) 478, 522, 847, 951, 1037
Bst4CI ACNGT 1 cut(s) 488
Bst6I CTCTTC 1 cut(s) 785
BstACI GRCGYC 1 cut(s) 141
BstAFI CTTAAG 1 cut(s) 449
BstC8I GCNNGC 2 cut(s) 85, 109
BstDEI CTNAG 2 cut(s) 459, 749
BstDSI CCRYGG 1 cut(s) 189
BstF5I GGATG 1 cut(s) 115
BstFNI CGCG 2 cut(s) 87, 191
BstH2I RGCGCY 1 cut(s) 84
BstHHI GCGC 2 cut(s) 83, 89
BstKTI GATC 7 cut(s) 175, 238, 397, 447, 532, 781, 1021
BstMAI GTCTC 1 cut(s) 876
BstMBI GATC 7 cut(s) 172, 235, 394, 444, 529, 778, 1018
BstMWI GCNNNNNNNGC 5 cut(s) 38, 41, 47, 56, 147
BstNI CCWGG 5 cut(s) 478, 522, 847, 951, 1037
BstNSI RCATGY 1 cut(s) 679
BstSCI CCNGG 6 cut(s) 118, 476, 520, 845, 949, 1035
BstSFI CTRYAG 1 cut(s) 1003
BstUI CGCG 2 cut(s) 87, 191
BstV1I GCAGC 5 cut(s) 18, 19, 28, 907, 1017
BstV2I GAAGAC 2 cut(s) 479, 812
BstX2I RGATCY 2 cut(s) 529, 1018
BstYI RGATCY 2 cut(s) 529, 1018
Bsu15I ATCGAT 1 cut(s) 62
BsuRI GGCC 4 cut(s) 50, 150, 481, 917
BsuTUI ATCGAT 1 cut(s) 62
BtgI CCRYGG 1 cut(s) 189
BtgZI GCGATG 1 cut(s) 723
BtsCI GGATG 1 cut(s) 115
BtsI GCAGTG 1 cut(s) 82
BtsIMutI CAGTG 2 cut(s) 82, 696
Cac8I GCNNGC 2 cut(s) 85, 109
CaiI CAGNNNCTG 1 cut(s) 1002
CciI TCATGA 1 cut(s) 781
CfoI GCGC 2 cut(s) 83, 89
Cfr13I GGNCC 1 cut(s) 518
Cfr42I CCGCGG 1 cut(s) 192
ClaI ATCGAT 1 cut(s) 62
CseI GACGC 1 cut(s) 130
Csp6I GTAC 1 cut(s) 205
CviAII CATG 7 cut(s) 170, 215, 225, 239, 356, 676, 782
CviQI GTAC 1 cut(s) 205
DdeI CTNAG 2 cut(s) 459, 749
DpnI GATC 7 cut(s) 174, 237, 396, 446, 531, 780, 1020
DpnII GATC 7 cut(s) 172, 235, 394, 444, 529, 778, 1018
DrdI GACNNNNNNGTC 1 cut(s) 161
DseDI GACNNNNNNGTC 1 cut(s) 161
EaeI YGGCCR 1 cut(s) 48
Eam1104I CTCTTC 1 cut(s) 785
EarI CTCTTC 1 cut(s) 785
Eco24I GRGCYC 1 cut(s) 214
Eco32I GATATC 2 cut(s) 543, 625
Eco47I GGWCC 1 cut(s) 518
EcoO109I RGGNCCY 1 cut(s) 518
EcoRII CCWGG 5 cut(s) 476, 520, 845, 949, 1035
EcoRV GATATC 2 cut(s) 543, 625
EcoT22I ATGCAT 1 cut(s) 61
EcoT38I GRGCYC 1 cut(s) 214
Esp3I CGTCTC 1 cut(s) 876
FaeI CATG 7 cut(s) 173, 218, 228, 242, 359, 679, 785
FalI AAGNNNNNCTT 2 cut(s) 804, 836
FaqI GGGAC 2 cut(s) 405, 531
FatI CATG 7 cut(s) 169, 214, 224, 238, 355, 675, 781
FauI CCCGC 1 cut(s) 498
FauNDI CATATG 1 cut(s) 900
FbaI TGATCA 1 cut(s) 235
Fnu4HI GCNGC 6 cut(s) 7, 33, 42, 918, 921, 1006
FokI GGATG 1 cut(s) 102
FriOI GRGCYC 1 cut(s) 214
Fsp4HI GCNGC 6 cut(s) 7, 33, 42, 918, 921, 1006
FspBI CTAG 3 cut(s) 72, 366, 1016
GlaI GCGC 2 cut(s) 82, 88
GluI GCNGC 6 cut(s) 7, 33, 42, 918, 921, 1006
GsuI CTGGAG 3 cut(s) 543, 972, 1019
HaeII RGCGCY 1 cut(s) 84
HaeIII GGCC 4 cut(s) 50, 150, 481, 917
HapII CCGG 1 cut(s) 120
HgaI GACGC 1 cut(s) 130
HhaI GCGC 2 cut(s) 83, 89
Hin1I GRCGYC 1 cut(s) 141
Hin1II CATG 7 cut(s) 173, 218, 228, 242, 359, 679, 785
Hin6I GCGC 2 cut(s) 81, 87
HinP1I GCGC 2 cut(s) 81, 87
HincII GTYRAC 1 cut(s) 439
HindII GTYRAC 1 cut(s) 439
HinfI GANTC 6 cut(s) 22, 128, 306, 725, 841, 1041
HpaII CCGG 1 cut(s) 120
Hpy166II GTNNAC 2 cut(s) 439, 736
Hpy188I TCNGA 6 cut(s) 21, 500, 534, 622, 730, 1046
Hpy188III TCNNGA 6 cut(s) 347, 553, 668, 776, 782, 824
Hpy8I GTNNAC 2 cut(s) 439, 736
HpyAV CCTTC 6 cut(s) 122, 638, 850, 856, 963, 1002
HpyCH4III ACNGT 1 cut(s) 488
HpyCH4IV ACGT 2 cut(s) 536, 691
HpyCH4V TGCA 7 cut(s) 44, 59, 353, 596, 679, 696, 1005
HpyF10VI GCNNNNNNNGC 5 cut(s) 38, 41, 47, 56, 147
HpyF3I CTNAG 2 cut(s) 459, 749
HpySE526I ACGT 2 cut(s) 536, 691
Hsp92I GRCGYC 1 cut(s) 141
Hsp92II CATG 7 cut(s) 173, 218, 228, 242, 359, 679, 785
HspAI GCGC 2 cut(s) 81, 87
Ksp22I TGATCA 1 cut(s) 235
KspI CCGCGG 1 cut(s) 192
Kzo9I GATC 7 cut(s) 172, 235, 394, 444, 529, 778, 1018
LmnI GCTCC 3 cut(s) 22, 381, 976
Lsp1109I GCAGC 5 cut(s) 18, 19, 28, 907, 1017
LweI GCATC 7 cut(s) 53, 68, 340, 393, 558, 700, 817
MaeI CTAG 3 cut(s) 72, 366, 1016
MaeII ACGT 2 cut(s) 536, 691
MaeIII GTNAC 2 cut(s) 217, 998
MalI GATC 7 cut(s) 174, 237, 396, 446, 531, 780, 1020
MboI GATC 7 cut(s) 172, 235, 394, 444, 529, 778, 1018
MboII GAAGA 6 cut(s) 241, 476, 484, 799, 802, 812
MflI RGATCY 2 cut(s) 529, 1018
MhlI GDGCHC 1 cut(s) 214
MluCI AATT 3 cut(s) 248, 294, 326
MlyI GAGTC 1 cut(s) 734
MmeI TCCRAC 2 cut(s) 50, 523
Mph1103I ATGCAT 1 cut(s) 61
MroXI GAANNNNTTC 1 cut(s) 578
MseI TTAA 3 cut(s) 54, 279, 450
MslI CAYNNNNRTG 1 cut(s) 489
MspA1I CMGCKG 5 cut(s) 32, 191, 920, 986, 1008
MspCI CTTAAG 1 cut(s) 449
MspI CCGG 1 cut(s) 120
MspR9I CCNGG 6 cut(s) 120, 478, 522, 847, 951, 1037
MvaI CCWGG 5 cut(s) 478, 522, 847, 951, 1037
MvnI CGCG 2 cut(s) 87, 191
MwoI GCNNNNNNNGC 5 cut(s) 38, 41, 47, 56, 147
NciI CCSGG 1 cut(s) 120
NdeI CATATG 1 cut(s) 900
NdeII GATC 7 cut(s) 172, 235, 394, 444, 529, 778, 1018
NlaIII CATG 7 cut(s) 173, 218, 228, 242, 359, 679, 785
NlaIV GGNNCC 2 cut(s) 18, 519
NsiI ATGCAT 1 cut(s) 61
NspI RCATGY 1 cut(s) 679
PagI TCATGA 1 cut(s) 781
PasI CCCWGGG 1 cut(s) 846
PcsI WCGNNNNNNNCGW 1 cut(s) 159
PdmI GAANNNNTTC 1 cut(s) 578
PfeI GAWTC 5 cut(s) 22, 128, 306, 841, 1041
PkrI GCNGC 6 cut(s) 8, 34, 43, 919, 922, 1007
PleI GAGTC 1 cut(s) 733
PpsI GAGTC 1 cut(s) 733
PpuMI RGGWCCY 1 cut(s) 518
PshBI ATTAAT 1 cut(s) 54
Psp1406I AACGTT 1 cut(s) 691
Psp5II RGGWCCY 1 cut(s) 518
Psp6I CCWGG 5 cut(s) 476, 520, 845, 949, 1035
PspGI CCWGG 5 cut(s) 476, 520, 845, 949, 1035
PspN4I GGNNCC 2 cut(s) 18, 519
PspPI GGNCC 1 cut(s) 518
PspPPI RGGWCCY 1 cut(s) 518
PstI CTGCAG 1 cut(s) 1007
PstNI CAGNNNCTG 1 cut(s) 1002
PsuI RGATCY 2 cut(s) 529, 1018
PvuII CAGCTG 1 cut(s) 986
RsaI GTAC 1 cut(s) 206
RsaNI GTAC 1 cut(s) 205
RseI CAYNNNNRTG 1 cut(s) 489
SacII CCGCGG 1 cut(s) 192
SaqAI TTAA 3 cut(s) 54, 279, 450
SatI GCNGC 6 cut(s) 7, 33, 42, 918, 921, 1006
Sau3AI GATC 7 cut(s) 172, 235, 394, 444, 529, 778, 1018
Sau96I GGNCC 1 cut(s) 518
SchI GAGTC 1 cut(s) 734
ScrFI CCNGG 6 cut(s) 120, 478, 522, 847, 951, 1037
SduI GDGCHC 1 cut(s) 214
SfaNI GCATC 7 cut(s) 53, 68, 340, 393, 558, 700, 817
SfcI CTRYAG 1 cut(s) 1003
Sfr303I CCGCGG 1 cut(s) 192
SgrBI CCGCGG 1 cut(s) 192
SinI GGWCC 1 cut(s) 518
SmiMI CAYNNNNRTG 1 cut(s) 489
SmlI CTYRAG 1 cut(s) 449
SmoI CTYRAG 1 cut(s) 449
Sse9I AATT 3 cut(s) 248, 294, 326
SsiI CCGC 7 cut(s) 30, 189, 191, 323, 505, 918, 1008
SspMI CTAG 3 cut(s) 72, 366, 1016
StyD4I CCNGG 6 cut(s) 118, 476, 520, 845, 949, 1035
TaaI ACNGT 1 cut(s) 488
TaiI ACGT 2 cut(s) 539, 694
TaqI TCGA 4 cut(s) 62, 153, 387, 1024
TasI AATT 3 cut(s) 248, 294, 326
TauI GCSGC 1 cut(s) 920
TfiI GAWTC 5 cut(s) 22, 128, 306, 841, 1041
Tru1I TTAA 3 cut(s) 54, 279, 450
Tru9I TTAA 3 cut(s) 54, 279, 450
TscAI CASTG 2 cut(s) 82, 703
TseI GCWGC 5 cut(s) 6, 32, 41, 920, 1005
TspDTI ATGAA 4 cut(s) 104, 213, 485, 903
TspRI CASTG 2 cut(s) 82, 703
Vha464I CTTAAG 1 cut(s) 449
VpaK11BI GGWCC 1 cut(s) 518
VspI ATTAAT 1 cut(s) 54
XceI RCATGY 1 cut(s) 679
XmnI GAANNNNTTC 1 cut(s) 578
XspI CTAG 3 cut(s) 72, 366, 1016
Zsp2I ATGCAT 1 cut(s) 61
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.