Prupe.1G070200_v2.0.a1

RNA helicase SDE3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0000595
Physical Location & Seq
Forward (+)
4978236 .. 4981724
3489 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G070200.1

Sequence Viewer

Length: 2310 bp
ATGTCACCGGTAAATATCAACTTGATGATGCGGGAATGGATTTGGGTACCTAGAAAATTGATTTTGAAGTCTCAGAGAGGTAGCAAAGCGCTCTTCAGTCGTTGGCTCGTTGACTCTGGTGAGTGTTTTTTTCTTCTTTTTTGGCTTTTTCTGTGTTTCTTTTTCCTTGTTGGTTTCTCAGTTCTGTTCTTGGATGGATCATATCCATCTTTAGACTGGAGGGCCAGCATGTCAATTTTTCTTGAAGTCTTGAAATGCCTTCTTTGTTGTGAAGATGAGCATGAGAATTATCATGGTTCTTACAATCAAATTCCCAGACAAACCAGTTTCCATCATAATGATTATGAATATCGAACTCCCCGTAGAAACTACTCGCCACCCCTGACTTCTACTAATCAATCTCCAGCCTTGTATAACTATAAACCCCCATCACCTCCACCTCAAGCACCTAAATCTCCTTTTAGTTCTTCTTCATCATCCCCTAAGCCTCCAACATCTTCATCGAAACCGCAGCAATCATCTTCTCCATCTCCTAATTTCCCAACATCTTTATCTAAAACACAACCATCTTCTTCTTTTTATCCATATTCAACATTCCCTGAGCCCCCATCATCTTCATCTAAGCCGCCTCAATCAACTTTTGTTAATCCATCTTCATCTAAACCACTACAATCCTCTGTCAATGCATCTTCATTTACTCATGAATTCCTAACAATTGTATTTAAATCACAAGAATCTTCTTCTGTTGATCAGCCTTCAGCATTACCTAAACCCCCAACATCTTCATCTAAGCTGCCCCAATCCACTCTTATTAGTCCATCTTCACCATCCCTTCAATTCCAAACACCTTCATCTAGACCATCTCCTCCTAAACTGTCTACAGCCTCCTCTAAACCACCTCAGGCTTCATCAAAATCACATTCATCATCACCAAAACCTCCAACATCTTCAAAACCATCTCCATCCTCAGGATCGCTGGTGTCTTCCACTAAGCTCCCTCCAATCTTCAAGCAAGTTCTATCCCCCGCCTGCCCGAGTGTAATAAATCAAAAGGTGAAGAAAAATTATGTGTGGGTCGAAAAGGATCCATTACCTATATTTCTGATTCCGGAGGATATCAAAGATTTGATCAAGAATGACATTGTGCCCAATGTTCTAAACCAGCCCCTGTCTCCCACAACATATAAGGATTACTTTGCCGCTCTGTTATATGCTGAAGAGTTCTACCATGAGAAATGGGCAGATTTCAATATGAAGAATGTGACACTGAAGTTGCATGAAGCAGCAGTTTATAAATATCTAGAGAAGGAAGAGAAAACCTTCATAGAATTTGAGATTGATTCTGTTCCTGAAAATAGGCCATTCCTTTTGTCAAGGGACATAGTCTATGTACGACCATCGGGTACGAATGTTGACCAATTTCAGGGCATTATATACCGCATTATTAGGAGCAGTCTTGTATTAGTGGAATTTGAAGATGAATTTTATGCTCATCATGATTCAACCCAAAAATATGATGTGAGCTTCTCATTCAATAGAGTCTGTTTGAAAAGAGCTCACCAAGCAGTTCAAACTGCATCAGATGCTTTATTTAAGAACTTCCTCTTTCCCGATTGTGTTTCTCGTACGAGCATCCCCACTGCACCAGCGCTGCTTTCTACCCATCCAGATAAGTTATCTGCAGTTCGTCGTATTTTAAGCATTCGGGGCTCACCGCCTTACCTAGTAGCTGGCTCGACAACTCGAGGGGTTGTTACTGAAGCAGTACGCCAGTTATGTCAAACCTCACCAGAGAATAAGTTTCTTATATGTGCTCCTAGTAACCGCTGTTGTGATGGGATTATGAGAAGCTTGTTGAAAGTGATTCCAGAGACAGATATATTTCGAGCCAATGCTGCATTTCGCGATAAAGATGAGGTGCCTGATGACATCCTCCCATCATGCCGTTACAAAGAGACTTATTTCTCTTGTCCTCCAACAGAGGAACTCCGAGAATTTAGGGTGATTTTCTCGACTTACGTAAGTAGCTTTCGGCTGCATGATAAAGGCCTAACCGCTGGACATTTTAGTCATATTTTTCTGGTGGATGCTTCATCTGCCATTGAGCCGGAAACAGCAGTGGCCTTAACTAATTTTGCAGAAAAAAGTACTACTGTTATAGTCACTGGTCAACCAGGAGATCATTCACGTTGGGTTCGCGCAGACATGGCAAGGGAAAGGGGGCTGAAGATTTCATACTTCGAGAGACTTTTCAAGTCAAGGCCATATAGAAGCCTCAATCCAATGCTTATCACACATTTGGACCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

770

Amino Acids

86.56

Weight (kDa)

8.97

Isoelectric Point (pI)

61.73

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000595)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g37100 FvH4_4g37100 FvH4_4g37100
malus_domestica MD13G1238300.v1.1 MD16G1243100.v1.1 MD16G1243200.v1.1
prunus_persica Prupe.1G070100_v2.0.a1 Prupe.1G070200_v2.0.a1 Prupe.1G070300_v2.0.a1 Prupe.1G070300_v2.0.a1
pyrus_communis pycom13g21070 pycom16g20390 pycom16g20400
rosa_chinensis RchiOBHm_Chr1g0318071 RchiOBHm_Chr1g0318111 RchiOBHm_Chr4g0446861 RchiOBHm_Chr4g0446881 RchiOBHm_Chr4g0446891
rosa_laevigata RLG00000005623 RLG00000005624 RLG00000009337 RLG00000030560 RLG00000030566
rosa_multiflora Rmu_co8177450.1_g000001 Rmu_sc0001753.1_g000012 Rmu_sc0001753.1_g000013 Rmu_sc0001753.1_g000014 Rmu_sc0001753.1_g000015 Rmu_sc0011424.1_g000009 Rmu_sc0011424.1_g000020 Rmu_sc0034555.1_g000001 Rmu_ssc0000008.1_g000002
rosa_roxburghii Rroxscaffold_4G00329530 Rroxscaffold_4G00329590 Rroxscaffold_4G00329800 Rroxscaffold_4G00329810 Rroxscaffold_4G00329860 Rroxscaffold_4G00329910 Rroxscaffold_4G00330030 Rroxscaffold_5G00343610 Rroxscaffold_5G00348280 Rroxscaffold_5G00387220 Rroxscaffold_5G00387230
rosa_rugosa Rorug01G0019100 Rorug01G0019100 Rorug01G0019500 Rorug01G0019600 Rorug04G0010100 Rorug04G0375200 Rorug04G0375300 Rorug04G0375400 Rorug04G0375500 Rorug04G0375600 Rorug04G0375700
rosa_samantha Rh1AG030700 Rh1AG030900 Rh1BG026900 Rh1CG029500 Rh1CG029700 Rh1DG040500 Rh4AG088100 Rh4AG436700 Rh4AG436800 Rh4AG436900 Rh4BG084700 Rh4BG445800 Rh4BG445900 Rh4BG446100 Rh4CG096300 Rh4CG463100 Rh4CG463200 Rh4CG463400 Rh4DG444500 Rh4DG444600 Rh4DG444700
rosa_wichuraiana Rw1G002420 Rw1G002440 Rw1G002480 Rw4G037360 Rw4G037370 Rw4G037380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1293
AasI GACNNNNNNGTC 1 cut(s) 2067
Acc65I GGTACC 1 cut(s) 46
AccB1I GGYRCC 2 cut(s) 46, 1918
AccBSI CCGCTC 1 cut(s) 1202
AccI GTMKAC 1 cut(s) 878
AccII CGCG 2 cut(s) 1905, 2199
AccIII TCCGGA 1 cut(s) 1108
AciI CCGC 9 cut(s) 31, 509, 626, 1026, 1200, 1438, 1715, 1825, 2055
AclWI GGATC 4 cut(s) 205, 979, 1079, 1092
AcsI RAATTY 6 cut(s) 309, 704, 1328, 1469, 1481, 1994
AcuI CTGAAG 6 cut(s) 79, 741, 1236, 1289, 1779, 2246
AfaI GTAC 6 cut(s) 48, 1392, 1405, 1627, 1767, 2149
AfeI AGCGCT 2 cut(s) 90, 1650
AfiI CCNNNNNNNGG 2 cut(s) 968, 1423
AgeI ACCGGT 1 cut(s) 7
AjnI CCWGG 1 cut(s) 2173
AluBI AGCT 7 cut(s) 793, 994, 1524, 1556, 1730, 1851, 2028
AluI AGCT 7 cut(s) 793, 994, 1524, 1556, 1730, 1851, 2028
Alw21I GWGCWC 2 cut(s) 1558, 1816
Alw26I GTCTC 5 cut(s) 75, 1176, 1865, 1949, 2239
AlwI GGATC 4 cut(s) 205, 979, 1079, 1092
AlwNI CAGNNNCTG 1 cut(s) 1168
Ama87I CYCGRG 2 cut(s) 1033, 1743
Aor13HI TCCGGA 1 cut(s) 1108
Aor51HI AGCGCT 2 cut(s) 90, 1650
AoxI GGCC 5 cut(s) 222, 1358, 2047, 2121, 2261
ApeKI GCWGC 6 cut(s) 511, 793, 1283, 1651, 1895, 2035
ApoI RAATTY 6 cut(s) 309, 704, 1328, 1469, 1481, 1994
AsiGI ACCGGT 1 cut(s) 7
Asp700I GAANNNNTTC 1 cut(s) 1319
Asp718I GGTACC 1 cut(s) 46
AspLEI GCGC 3 cut(s) 91, 1651, 2201
AspS9I GGNCC 2 cut(s) 222, 2302
AsuHPI GGTGA 9 cut(s) 131, 423, 816, 921, 1066, 1550, 1704, 1779, 2014
AvaI CYCGRG 2 cut(s) 1033, 1743
AvaII GGWCC 1 cut(s) 2302
AxyI CCTNAGG 2 cut(s) 900, 967
BaeGI GKGCMC 1 cut(s) 1149
BaeI ACNNNNGTAYC 2 cut(s) 1395, 1428
BamHI GGATCC 1 cut(s) 1084
BanI GGYRCC 2 cut(s) 46, 1918
BanII GRGCYC 3 cut(s) 606, 1558, 1712
BarI GAAGNNNNNNTAC 2 cut(s) 2219, 2251
BbsI GAAGAC 1 cut(s) 975
Bbv12I GWGCWC 2 cut(s) 1558, 1816
BbvI GCAGC 6 cut(s) 523, 780, 1295, 1638, 1882, 2022
BceAI ACGGC 1 cut(s) 1929
BciT130I CCWGG 1 cut(s) 2175
BclI TGATCA 2 cut(s) 748, 1128
BcoDI GTCTC 5 cut(s) 75, 1176, 1865, 1949, 2239
BfaI CTAG 5 cut(s) 51, 855, 1301, 1724, 1818
BfmI CTRYAG 2 cut(s) 879, 1680
BfoI RGCGCY 2 cut(s) 92, 1652
BisI GCNGC 8 cut(s) 512, 626, 794, 1200, 1284, 1652, 1896, 2036
BlsI GCNGC 8 cut(s) 513, 627, 795, 1201, 1285, 1653, 1897, 2037
BmcAI AGTACT 1 cut(s) 2149
Bme1390I CCNGG 1 cut(s) 2175
Bme18I GGWCC 1 cut(s) 2302
BmeT110I CYCGRG 2 cut(s) 1033, 1743
BmgT120I GGNCC 2 cut(s) 222, 2302
BmiI GGNNCC 3 cut(s) 48, 1086, 1920
BmrFI CCNGG 1 cut(s) 2175
BmsI GCATC 6 cut(s) 18, 695, 1573, 1586, 1641, 2077
BpiI GAAGAC 1 cut(s) 975
BpmI CTGGAG 2 cut(s) 238, 387
Bpu10I CCTNAGC 2 cut(s) 483, 600
BpuEI CTTGAG 1 cut(s) 426
BsaAI YACGTR 1 cut(s) 2020
BsaWI WCCGGW 2 cut(s) 7, 1108
Bsc4I CCNNNNNNNGG 2 cut(s) 968, 1423
Bse118I RCCGGY 1 cut(s) 7
Bse1I ACTGG 4 cut(s) 221, 324, 1771, 2170
Bse21I CCTNAGG 2 cut(s) 900, 967
BseAI TCCGGA 1 cut(s) 1108
BseBI CCWGG 1 cut(s) 2175
BseGI GGATG 8 cut(s) 199, 476, 827, 962, 1632, 1663, 1929, 2092
BseLI CCNNNNNNNGG 2 cut(s) 968, 1423
BseMII CTCAG 5 cut(s) 86, 192, 591, 914, 981
BseNI ACTGG 4 cut(s) 221, 324, 1771, 2170
BseRI GAGGAG 2 cut(s) 855, 877
BseSI GKGCMC 1 cut(s) 1149
BseXI GCAGC 6 cut(s) 523, 780, 1295, 1638, 1882, 2022
BsgI GTGCAG 1 cut(s) 1626
Bsh1236I CGCG 2 cut(s) 1905, 2199
BshFI GGCC 5 cut(s) 224, 1360, 2049, 2123, 2263
BshNI GGYRCC 2 cut(s) 46, 1918
BshTI ACCGGT 1 cut(s) 7
BsiHKAI GWGCWC 2 cut(s) 1558, 1816
BsiHKCI CYCGRG 2 cut(s) 1033, 1743
BsiSI CCGG 3 cut(s) 8, 1109, 2108
BsiWI CGTACG 1 cut(s) 1625
BslFI GGGAC 1 cut(s) 1391
BslI CCNNNNNNNGG 2 cut(s) 968, 1423
BsmAI GTCTC 5 cut(s) 75, 1176, 1865, 1949, 2239
BsmFI GGGAC 1 cut(s) 1391
BsmI GAATGC 1 cut(s) 1701
BsnI GGCC 5 cut(s) 224, 1360, 2049, 2123, 2263
BsoBI CYCGRG 2 cut(s) 1033, 1743
Bsp1286I GDGCHC 5 cut(s) 606, 1149, 1558, 1712, 1816
Bsp13I TCCGGA 1 cut(s) 1108
Bsp143I GATC 6 cut(s) 197, 748, 971, 1084, 1128, 2179
Bsp68I TCGCGA 1 cut(s) 1905
BspACI CCGC 9 cut(s) 31, 509, 626, 1026, 1200, 1438, 1715, 1825, 2055
BspANI GGCC 5 cut(s) 224, 1360, 2049, 2123, 2263
BspCNI CTCAG 5 cut(s) 85, 191, 592, 913, 980
BspEI TCCGGA 1 cut(s) 1108
BspFNI CGCG 2 cut(s) 1905, 2199
BspHI TCATGA 2 cut(s) 700, 1495
BspLI GGNNCC 3 cut(s) 48, 1086, 1920
BspMAI CTGCAG 1 cut(s) 1684
BspPI GGATC 4 cut(s) 205, 979, 1079, 1092
BspQI GCTCTTC 1 cut(s) 98
BspT107I GGYRCC 2 cut(s) 46, 1918
BsrBI CCGCTC 1 cut(s) 1202
BsrFI RCCGGY 1 cut(s) 7
BsrI ACTGG 4 cut(s) 221, 324, 1771, 2170
BssAI RCCGGY 1 cut(s) 7
BssMI GATC 6 cut(s) 197, 748, 971, 1084, 1128, 2179
Bst2UI CCWGG 1 cut(s) 2175
Bst4CI ACNGT 2 cut(s) 876, 2155
Bst6I CTCTTC 3 cut(s) 98, 1212, 1305
BstAPI GCANNNNNTGC 1 cut(s) 1583
BstBAI YACGTR 1 cut(s) 2020
BstC8I GCNNGC 3 cut(s) 226, 1030, 1732
BstDEI CTNAG 9 cut(s) 72, 178, 483, 600, 621, 789, 900, 967, 990
BstF5I GGATG 8 cut(s) 199, 476, 827, 962, 1632, 1663, 1929, 2092
BstFNI CGCG 2 cut(s) 1905, 2199
BstH2I RGCGCY 2 cut(s) 92, 1652
BstHHI GCGC 3 cut(s) 91, 1651, 2201
BstKTI GATC 6 cut(s) 200, 751, 974, 1087, 1131, 2182
BstMAI GTCTC 5 cut(s) 75, 1176, 1865, 1949, 2239
BstMBI GATC 6 cut(s) 197, 748, 971, 1084, 1128, 2179
BstMWI GCNNNNNNNGC 6 cut(s) 1562, 1583, 1707, 1895, 2096, 2207
BstNI CCWGG 1 cut(s) 2175
BstNSI RCATGY 1 cut(s) 232
BstSCI CCNGG 1 cut(s) 2173
BstSFI CTRYAG 2 cut(s) 879, 1680
BstSLI GKGCMC 1 cut(s) 1149
BstSNI TACGTA 1 cut(s) 2020
BstUI CGCG 2 cut(s) 1905, 2199
BstV1I GCAGC 6 cut(s) 523, 780, 1295, 1638, 1882, 2022
BstV2I GAAGAC 1 cut(s) 975
BstX2I RGATCY 1 cut(s) 1084
BstYI RGATCY 1 cut(s) 1084
Bsu36I CCTNAGG 2 cut(s) 900, 967
BsuRI GGCC 5 cut(s) 224, 1360, 2049, 2123, 2263
BtsCI GGATG 8 cut(s) 199, 476, 827, 962, 1632, 1663, 1929, 2092
BtsI GCAGTG 2 cut(s) 1638, 2124
BtsIMutI CAGTG 4 cut(s) 1265, 1638, 2124, 2163
BtuMI TCGCGA 1 cut(s) 1905
Cac8I GCNNGC 3 cut(s) 226, 1030, 1732
CaiI CAGNNNCTG 1 cut(s) 1168
CciI TCATGA 2 cut(s) 700, 1495
CfoI GCGC 3 cut(s) 91, 1651, 2201
Cfr10I RCCGGY 1 cut(s) 7
Cfr13I GGNCC 2 cut(s) 222, 2302
Csp6I GTAC 6 cut(s) 47, 1391, 1404, 1626, 1766, 2148
CspAI ACCGGT 1 cut(s) 7
CviQI GTAC 6 cut(s) 47, 1391, 1404, 1626, 1766, 2148
DdeI CTNAG 9 cut(s) 72, 178, 483, 600, 621, 789, 900, 967, 990
DpnI GATC 6 cut(s) 199, 750, 973, 1086, 1130, 2181
DpnII GATC 6 cut(s) 197, 748, 971, 1084, 1128, 2179
DraI TTTAAA 1 cut(s) 724
DrdI GACNNNNNNGTC 1 cut(s) 2067
DseDI GACNNNNNNGTC 1 cut(s) 2067
Eam1104I CTCTTC 3 cut(s) 98, 1212, 1305
EarI CTCTTC 3 cut(s) 98, 1212, 1305
Ecl136II GAGCTC 1 cut(s) 1556
Eco105I TACGTA 1 cut(s) 2020
Eco147I AGGCCT 1 cut(s) 2049
Eco24I GRGCYC 3 cut(s) 606, 1558, 1712
Eco32I GATATC 1 cut(s) 1117
Eco47I GGWCC 1 cut(s) 2302
Eco47III AGCGCT 2 cut(s) 90, 1650
Eco53kI GAGCTC 1 cut(s) 1556
Eco57I CTGAAG 6 cut(s) 79, 741, 1236, 1289, 1779, 2246
Eco81I CCTNAGG 2 cut(s) 900, 967
Eco88I CYCGRG 2 cut(s) 1033, 1743
EcoICRI GAGCTC 1 cut(s) 1556
EcoRI GAATTC 1 cut(s) 704
EcoRII CCWGG 1 cut(s) 2173
EcoRV GATATC 1 cut(s) 1117
EcoT22I ATGCAT 1 cut(s) 688
EcoT38I GRGCYC 3 cut(s) 606, 1558, 1712
FalI AAGNNNNNCTT 2 cut(s) 1178, 1210
FaqI GGGAC 1 cut(s) 1391
FauI CCCGC 2 cut(s) 24, 1033
FbaI TGATCA 2 cut(s) 748, 1128
FblI GTMKAC 1 cut(s) 878
Fnu4HI GCNGC 8 cut(s) 512, 626, 794, 1200, 1284, 1652, 1896, 2036
FokI GGATG 8 cut(s) 206, 463, 814, 949, 1619, 1650, 1916, 2099
FriOI GRGCYC 3 cut(s) 606, 1558, 1712
Fsp4HI GCNGC 8 cut(s) 512, 626, 794, 1200, 1284, 1652, 1896, 2036
FspBI CTAG 5 cut(s) 51, 855, 1301, 1724, 1818
GlaI GCGC 3 cut(s) 90, 1650, 2200
GluI GCNGC 8 cut(s) 512, 626, 794, 1200, 1284, 1652, 1896, 2036
GsuI CTGGAG 2 cut(s) 238, 387
HaeII RGCGCY 2 cut(s) 92, 1652
HaeIII GGCC 5 cut(s) 224, 1360, 2049, 2123, 2263
HapII CCGG 3 cut(s) 8, 1109, 2108
HhaI GCGC 3 cut(s) 91, 1651, 2201
Hin6I GCGC 3 cut(s) 89, 1649, 2199
HinP1I GCGC 3 cut(s) 89, 1649, 2199
HincII GTYRAC 3 cut(s) 112, 1414, 2171
HindII GTYRAC 3 cut(s) 112, 1414, 2171
HindIII AAGCTT 1 cut(s) 1849
HinfI GANTC 7 cut(s) 113, 734, 1105, 1340, 1499, 1539, 1864
HpaII CCGG 3 cut(s) 8, 1109, 2108
HphI GGTGA 9 cut(s) 131, 423, 816, 921, 1066, 1550, 1704, 1779, 2014
Hpy166II GTNNAC 4 cut(s) 112, 879, 1414, 2171
Hpy188I TCNGA 4 cut(s) 75, 1104, 1582, 1991
Hpy8I GTNNAC 4 cut(s) 112, 879, 1414, 2171
Hpy99I CGWCG 1 cut(s) 1692
HpyAV CCTTC 6 cut(s) 269, 765, 842, 858, 1300, 1330
HpyCH4III ACNGT 2 cut(s) 876, 2155
HpyCH4IV ACGT 2 cut(s) 2019, 2188
HpyCH4V TGCA 8 cut(s) 686, 1276, 1577, 1643, 1682, 1898, 2038, 2138
HpyF10VI GCNNNNNNNGC 6 cut(s) 1562, 1583, 1707, 1895, 2096, 2207
HpyF3I CTNAG 9 cut(s) 72, 178, 483, 600, 621, 789, 900, 967, 990
HpySE526I ACGT 2 cut(s) 2019, 2188
HspAI GCGC 3 cut(s) 89, 1649, 2199
Kpn2I TCCGGA 1 cut(s) 1108
KpnI GGTACC 1 cut(s) 50
Ksp22I TGATCA 2 cut(s) 748, 1128
Kzo9I GATC 6 cut(s) 197, 748, 971, 1084, 1128, 2179
LguI GCTCTTC 1 cut(s) 98
LmnI GCTCC 3 cut(s) 999, 1449, 1819
Lsp1109I GCAGC 6 cut(s) 523, 780, 1295, 1638, 1882, 2022
LweI GCATC 6 cut(s) 18, 695, 1573, 1586, 1641, 2077
MaeI CTAG 5 cut(s) 51, 855, 1301, 1724, 1818
MaeII ACGT 2 cut(s) 2019, 2188
MaeIII GTNAC 6 cut(s) 3, 1261, 1753, 1820, 1946, 2161
MalI GATC 6 cut(s) 199, 750, 973, 1086, 1130, 2181
MbiI CCGCTC 1 cut(s) 1202
MboI GATC 6 cut(s) 197, 748, 971, 1084, 1128, 2179
MfeI CAATTG 1 cut(s) 714
MflI RGATCY 1 cut(s) 1084
MhlI GDGCHC 5 cut(s) 606, 1149, 1558, 1712, 1816
MlyI GAGTC 2 cut(s) 107, 1548
MmeI TCCRAC 3 cut(s) 515, 965, 2000
Mph1103I ATGCAT 1 cut(s) 688
MroI TCCGGA 1 cut(s) 1108
MroXI GAANNNNTTC 1 cut(s) 1319
MseI TTAA 5 cut(s) 645, 723, 1593, 1697, 2126
MslI CAYNNNNRTG 1 cut(s) 336
MspA1I CMGCKG 2 cut(s) 1827, 2057
MspI CCGG 3 cut(s) 8, 1109, 2108
MspR9I CCNGG 1 cut(s) 2175
MunI CAATTG 1 cut(s) 714
Mva1269I GAATGC 1 cut(s) 1701
MvaI CCWGG 1 cut(s) 2175
MvnI CGCG 2 cut(s) 1905, 2199
MwoI GCNNNNNNNGC 6 cut(s) 1562, 1583, 1707, 1895, 2096, 2207
NdeII GATC 6 cut(s) 197, 748, 971, 1084, 1128, 2179
NlaIV GGNNCC 3 cut(s) 48, 1086, 1920
NmuCI GTSAC 3 cut(s) 3, 1261, 2161
NruI TCGCGA 1 cut(s) 1905
NsiI ATGCAT 1 cut(s) 688
NspI RCATGY 1 cut(s) 232
PaeR7I CTCGAG 1 cut(s) 1743
PagI TCATGA 2 cut(s) 700, 1495
PceI AGGCCT 1 cut(s) 2049
PciSI GCTCTTC 1 cut(s) 98
PcsI WCGNNNNNNNCGW 1 cut(s) 358
PctI GAATGC 1 cut(s) 1701
PdmI GAANNNNTTC 1 cut(s) 1319
PfeI GAWTC 5 cut(s) 734, 1105, 1340, 1499, 1864
Pfl23II CGTACG 1 cut(s) 1625
PflFI GACNNNGTC 1 cut(s) 1382
PinAI ACCGGT 1 cut(s) 7
PkrI GCNGC 8 cut(s) 513, 627, 795, 1201, 1285, 1653, 1897, 2037
PleI GAGTC 2 cut(s) 107, 1547
PpsI GAGTC 2 cut(s) 107, 1547
Ppu21I YACGTR 1 cut(s) 2020
PsiI TTATAA 1 cut(s) 1293
Psp124BI GAGCTC 1 cut(s) 1558
Psp6I CCWGG 1 cut(s) 2173
PspGI CCWGG 1 cut(s) 2173
PspLI CGTACG 1 cut(s) 1625
PspN4I GGNNCC 3 cut(s) 48, 1086, 1920
PspPI GGNCC 2 cut(s) 222, 2302
PspXI VCTCGAGB 1 cut(s) 1743
PstI CTGCAG 1 cut(s) 1684
PstNI CAGNNNCTG 1 cut(s) 1168
PsuI RGATCY 1 cut(s) 1084
PsyI GACNNNGTC 1 cut(s) 1382
RruI TCGCGA 1 cut(s) 1905
RsaI GTAC 6 cut(s) 48, 1392, 1405, 1627, 1767, 2149
RsaNI GTAC 6 cut(s) 47, 1391, 1404, 1626, 1766, 2148
RseI CAYNNNNRTG 1 cut(s) 336
SacI GAGCTC 1 cut(s) 1558
SapI GCTCTTC 1 cut(s) 98
SaqAI TTAA 5 cut(s) 645, 723, 1593, 1697, 2126
SatI GCNGC 8 cut(s) 512, 626, 794, 1200, 1284, 1652, 1896, 2036
Sau3AI GATC 6 cut(s) 197, 748, 971, 1084, 1128, 2179
Sau96I GGNCC 2 cut(s) 222, 2302
ScaI AGTACT 1 cut(s) 2149
SchI GAGTC 2 cut(s) 107, 1548
ScrFI CCNGG 1 cut(s) 2175
SduI GDGCHC 5 cut(s) 606, 1149, 1558, 1712, 1816
SfaNI GCATC 6 cut(s) 18, 695, 1573, 1586, 1641, 2077
SfcI CTRYAG 2 cut(s) 879, 1680
Sfr274I CTCGAG 1 cut(s) 1743
SinI GGWCC 1 cut(s) 2302
SlaI CTCGAG 1 cut(s) 1743
SmiI ATTTAAAT 1 cut(s) 724
SmiMI CAYNNNNRTG 1 cut(s) 336
SmlI CTYRAG 2 cut(s) 441, 1743
SmoI CTYRAG 2 cut(s) 441, 1743
SnaBI TACGTA 1 cut(s) 2020
SseBI AGGCCT 1 cut(s) 2049
SsiI CCGC 9 cut(s) 31, 509, 626, 1026, 1200, 1438, 1715, 1825, 2055
SspMI CTAG 5 cut(s) 51, 855, 1301, 1724, 1818
SstI GAGCTC 1 cut(s) 1558
StuI AGGCCT 1 cut(s) 2049
StyD4I CCNGG 1 cut(s) 2173
SwaI ATTTAAAT 1 cut(s) 724
TaaI ACNGT 2 cut(s) 876, 2155
TaiI ACGT 2 cut(s) 2022, 2191
TaqI TCGA 8 cut(s) 352, 503, 1077, 1736, 1744, 1885, 2012, 2241
TatI WGTACW 1 cut(s) 2147
TauI GCSGC 2 cut(s) 628, 1202
TfiI GAWTC 5 cut(s) 734, 1105, 1340, 1499, 1864
Tru1I TTAA 5 cut(s) 645, 723, 1593, 1697, 2126
Tru9I TTAA 5 cut(s) 645, 723, 1593, 1697, 2126
TscAI CASTG 4 cut(s) 1272, 1645, 2124, 2170
TseFI GTSAC 3 cut(s) 3, 1261, 2161
TseI GCWGC 6 cut(s) 511, 793, 1283, 1651, 1895, 2035
Tsp45I GTSAC 3 cut(s) 3, 1261, 2161
TspRI CASTG 4 cut(s) 1272, 1645, 2124, 2170
Tth111I GACNNNGTC 1 cut(s) 1382
VpaK11BI GGWCC 1 cut(s) 2302
XapI RAATTY 6 cut(s) 309, 704, 1328, 1469, 1481, 1994
XbaI TCTAGA 2 cut(s) 854, 1300
XceI RCATGY 1 cut(s) 232
XcmI CCANNNNNNNNNTGG 1 cut(s) 213
XhoI CTCGAG 1 cut(s) 1743
XmiI GTMKAC 1 cut(s) 878
XmnI GAANNNNTTC 1 cut(s) 1319
XspI CTAG 5 cut(s) 51, 855, 1301, 1724, 1818
ZrmI AGTACT 1 cut(s) 2149
Zsp2I ATGCAT 1 cut(s) 688
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.