RchiOBHm_Chr4g0446861

RNA helicase SDE3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0000595
Physical Location & Seq
Reverse (-)
66841630 .. 66844598
2969 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ41434

Sequence Viewer

Length: 2028 bp
ATGTCATGCTTTTTTGATCTTCTGAGATGCCTTCTGTGTTGTTCTGATGAGCCTGATTTTCATCGAACTTCTTCCCGTTATACAAGAAACCGTTTTGTTGATACCGCTGACCTTCACTATTATGATCCCATTCCCAGTCCCAGGGGCAGCCATTTGCCAACTTCAACTTCATTTCAGCCTGAGTACTATTCTCGTGATTTTGTTAATTCTCTACTTCATTCTCCTGCTTCACCTTCCACCACCTCCTCTTATAAACCACCACCACCTCAATCAACAACACCACCACCAGCATCTCAATATTATTCTTCTAATAACAAACCGCCGGTCACTTCTTTTATCAGTTCATCTTCATCATCTAAACCAACCCTATCGTCTACTATTAAACCATCTTCATCATCCTCTAAGAAGCCACCCCAGCCTCGGCCTCAGCCAACATCATCTAATTCTAAACCAAATCCATCCTCCCAAAAACCTCCATCACATTCTTCATCTCCATCTTCTCCAAAGCTCCCTCCTGTCTACAAGTCAATTCTATCCCCAGCCTCCTCCTCCTCCTTCTCCCCAAATGTTACAAGTCAACACCAACAAGACGGGAAGAAAACAGCTTATGTGTGTGTTGAAGAGGATCCATTACCTGTATTCATGATTCCTGAGGATATCAAAGCCTTGATCAAGAACAAAATTGTGCCCAAAGTTCTGATTCGCCCATTATCCCCCACAACATACAAGGACTACTTTGCCGCTCTCTTATATTCTGAAGATTTCTACTTGGAGCAATGGAGTGATTTCCTTTTGAAGGGTGTGACATTGGGGTTGGATGAAAGTTCAATTTATAAAATTCGGAAAAAGGAAGCAATTTATAAAAATCGGAAAAAGGAAGATAAGGAAGATAAAATCTTTGTAGCATTTGAGCTTGATTCTGTTCCTGAGGAACGGCCATTCCTCTTATCAAGGGACTTGGTCTTTGCACGACCAGTGGGTAACAGTACTGCTGAACCCTTCAAGGGCTTCATCTATAAAGTTGTTGGGAGCAAACGCGTTTTGGTTGAATTTGAAGATGGCTTTTATTCTAATCATCATTCCAACAAAAAATATGATATCAGCTTCTCATTCAATAGAGTTTGTTTGAAAAGAGCCCACCATGCAGTTAAAGCTGCATCAGACGCCTTGTTTCAGAACTTCCTCTTCCCTGACACTGACTCACGAGCAAGCATTCCTACTGCACCAGCTCTGCTTTCTACTACCTGTCATAAGCTTGATCAAGATCAATGTTGTGCGGTTGGACATATCTTAAGGATTCAGGGCTCACCACCTTATGTAGTAACTGGTCCAGACTGTGAAGGCGAATCTTTTGCATGTTTAAGAGAACCTTCAAAAACTGAAGGCAAATCTTTTGCATATTTAAGAGAACCTTCAAAAACTGGAGTGGTTGTTAGTGAAGCAGTATACCAATTATGTCAGAAGTCGCCAAAGTATCGGATTCTTATCTGTGCGCCTACTAACAGCTGCTGTGATGTGCTGATGAGAAGCTTGGTGAAGGTGATTCCAGAGTCGACTATGTTTCGTGCCAATGCTGCATTCCGTGAGAAAGAAGAGGTACCTGAGGATATCCTCCGCTCATCCCTTTACAAAGAGTCTTGTTTTTCTTGTCCTTCAATTGAGAAACTCCGGAAATACAGGGTGATTTTCTCGACTTTCATGAGTAGCTTTCGACTACATGATAAAGGCATAGCTGTTGGACATTTTAGCCATATTTTTCTGGTGGATGCTTCATCCGCTATTGAACCGGAAACATTGGTCGCCCTGACTAACTTTGCCGACAAGACTACTAGTGTTATAGTTACTGGTAAACCTGGAAACAGTCCACGCTGGGTTCGCGCTGACATTGCAAGGAAAAAGGGACTCAAGATTTCATACTTTGAAAGACTCTGTAAGCTCAGCCCCTATAGAAGCCGCAGTCCAATGTTCATCACACAATTAGACCTGAACCAAAAGTCAGGCTCAGACTACCAAACAAGAACTTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

675

Amino Acids

75.65

Weight (kDa)

9.13

Isoelectric Point (pI)

59.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MOV-10_beta-barrel PF21634 293 - 369 1.9e-09 Helicase MOV-10, beta-barrel domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000595)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g37100 FvH4_4g37100 FvH4_4g37100
malus_domestica MD13G1238300.v1.1 MD16G1243100.v1.1 MD16G1243200.v1.1
prunus_persica Prupe.1G070100_v2.0.a1 Prupe.1G070200_v2.0.a1 Prupe.1G070300_v2.0.a1 Prupe.1G070300_v2.0.a1
pyrus_communis pycom13g21070 pycom16g20390 pycom16g20400
rosa_chinensis RchiOBHm_Chr1g0318071 RchiOBHm_Chr1g0318111 RchiOBHm_Chr4g0446861 RchiOBHm_Chr4g0446881 RchiOBHm_Chr4g0446891
rosa_laevigata RLG00000005623 RLG00000005624 RLG00000009337 RLG00000030560 RLG00000030566
rosa_multiflora Rmu_co8177450.1_g000001 Rmu_sc0001753.1_g000012 Rmu_sc0001753.1_g000013 Rmu_sc0001753.1_g000014 Rmu_sc0001753.1_g000015 Rmu_sc0011424.1_g000009 Rmu_sc0011424.1_g000020 Rmu_sc0034555.1_g000001 Rmu_ssc0000008.1_g000002
rosa_roxburghii Rroxscaffold_4G00329530 Rroxscaffold_4G00329590 Rroxscaffold_4G00329800 Rroxscaffold_4G00329810 Rroxscaffold_4G00329860 Rroxscaffold_4G00329910 Rroxscaffold_4G00330030 Rroxscaffold_5G00343610 Rroxscaffold_5G00348280 Rroxscaffold_5G00387220 Rroxscaffold_5G00387230
rosa_rugosa Rorug01G0019100 Rorug01G0019100 Rorug01G0019500 Rorug01G0019600 Rorug04G0010100 Rorug04G0375200 Rorug04G0375300 Rorug04G0375400 Rorug04G0375500 Rorug04G0375600 Rorug04G0375700
rosa_samantha Rh1AG030700 Rh1AG030900 Rh1BG026900 Rh1CG029500 Rh1CG029700 Rh1DG040500 Rh4AG088100 Rh4AG436700 Rh4AG436800 Rh4AG436900 Rh4BG084700 Rh4BG445800 Rh4BG445900 Rh4BG446100 Rh4CG096300 Rh4CG463100 Rh4CG463200 Rh4CG463400 Rh4DG444500 Rh4DG444600 Rh4DG444700
rosa_wichuraiana Rw1G002420 Rw1G002440 Rw1G002480 Rw4G037360 Rw4G037370 Rw4G037380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 252, 834, 861
Acc65I GGTACC 1 cut(s) 1597
AccB1I GGYRCC 1 cut(s) 1597
AccBSI CCGCTC 2 cut(s) 743, 1617
AccI GTMKAC 4 cut(s) 374, 519, 1446, 1553
AccII CGCG 2 cut(s) 1038, 1878
AccIII TCCGGA 1 cut(s) 1668
AciI CCGC 7 cut(s) 105, 320, 741, 1277, 1615, 1776, 1954
AclWI GGATC 3 cut(s) 119, 620, 633
AcoI YGGCCR 1 cut(s) 935
AcsI RAATTY 2 cut(s) 837, 1049
AcuI CTGAAG 2 cut(s) 777, 1401
AcyI GRCGYC 1 cut(s) 1164
AfaI GTAC 3 cut(s) 185, 988, 1599
AfiI CCNNNNNNNGG 4 cut(s) 141, 420, 796, 1004
AflII CTTAAG 1 cut(s) 1291
AflIII ACRYGT 1 cut(s) 1036
AhlI ACTAGT 1 cut(s) 1829
AjnI CCWGG 2 cut(s) 140, 1852
AjuI GAANNNNNNNTTGG 4 cut(s) 151, 183, 1562, 1594
AloI GAACNNNNNNTCC 2 cut(s) 924, 956
AlwI GGATC 3 cut(s) 119, 620, 633
AlwNI CAGNNNCTG 1 cut(s) 1509
Aor13HI TCCGGA 1 cut(s) 1668
AoxI GGCC 2 cut(s) 422, 935
ApeKI GCWGC 4 cut(s) 147, 1154, 1506, 1574
ApoI RAATTY 2 cut(s) 837, 1049
ArsI GACNNNNNNTTYG 4 cut(s) 309, 341, 947, 979
Asp700I GAANNNNTTC 1 cut(s) 70
Asp718I GGTACC 1 cut(s) 1597
AspLEI GCGC 2 cut(s) 1495, 1880
AspS9I GGNCC 1 cut(s) 1328
AsuHPI GGTGA 5 cut(s) 222, 1299, 1546, 1552, 1693
AvaII GGWCC 1 cut(s) 1328
AxyI CCTNAGG 3 cut(s) 651, 927, 1602
BaeGI GKGCMC 1 cut(s) 690
BamHI GGATCC 1 cut(s) 625
BanI GGYRCC 1 cut(s) 1597
BanII GRGCYC 2 cut(s) 1138, 1307
BauI CACGAG 2 cut(s) 192, 1203
BbvCI CCTCAGC 1 cut(s) 426
BbvI GCAGC 4 cut(s) 159, 1141, 1493, 1561
BccI CCATC 5 cut(s) 394, 466, 484, 502, 1052
BceAI ACGGC 1 cut(s) 950
BcgI CGANNNNNNTGC 2 cut(s) 1334, 1368
BciT130I CCWGG 2 cut(s) 142, 1854
BclI TGATCA 2 cut(s) 669, 1258
BcuI ACTAGT 1 cut(s) 1829
BfaI CTAG 1 cut(s) 1830
BfmI CTRYAG 1 cut(s) 1945
BfrI CTTAAG 1 cut(s) 1291
BisI GCNGC 6 cut(s) 148, 741, 1155, 1507, 1575, 1954
BlpI GCTNAGC 1 cut(s) 1937
BlsI GCNGC 6 cut(s) 149, 742, 1156, 1508, 1576, 1955
BmcAI AGTACT 2 cut(s) 185, 988
Bme1390I CCNGG 2 cut(s) 142, 1854
Bme18I GGWCC 1 cut(s) 1328
BmgT120I GGNCC 1 cut(s) 1328
BmiI GGNNCC 2 cut(s) 627, 1599
BmrFI CCNGG 2 cut(s) 142, 1854
BmrI ACTGGG 1 cut(s) 129
BmsI GCATC 4 cut(s) 17, 299, 1166, 1756
BmuI ACTGGG 1 cut(s) 129
BpmI CTGGAG 1 cut(s) 1443
Bpu10I CCTNAGC 1 cut(s) 426
Bpu1102I GCTNAGC 1 cut(s) 1937
BpuEI CTTGAG 1 cut(s) 1889
BsaBI GATNNNNATC 3 cut(s) 60, 1263, 1484
BsaHI GRCGYC 1 cut(s) 1164
BsaJI CCNNGG 3 cut(s) 140, 141, 419
BsaWI WCCGGW 2 cut(s) 1668, 1786
BsaXI ACNNNNNCTCC 2 cut(s) 1416, 1446
Bsc4I CCNNNNNNNGG 4 cut(s) 141, 420, 796, 1004
Bse118I RCCGGY 1 cut(s) 322
Bse1I ACTGG 5 cut(s) 135, 974, 1330, 1426, 1849
Bse21I CCTNAGG 3 cut(s) 651, 927, 1602
Bse3DI GCAATG 2 cut(s) 782, 1884
Bse8I GATNNNNATC 3 cut(s) 60, 1263, 1484
BseAI TCCGGA 1 cut(s) 1668
BseBI CCWGG 2 cut(s) 142, 1854
BseDI CCNNGG 3 cut(s) 140, 141, 419
BseGI GGATG 6 cut(s) 395, 458, 823, 1619, 1771, 1772
BseJI GATNNNNATC 3 cut(s) 60, 1263, 1484
BseLI CCNNNNNNNGG 4 cut(s) 141, 420, 796, 1004
BseMI GCAATG 2 cut(s) 782, 1884
BseMII CTCAG 8 cut(s) 14, 171, 440, 642, 918, 1593, 1951, 2016
BseNI ACTGG 5 cut(s) 135, 974, 1330, 1426, 1849
BseRI GAGGAG 4 cut(s) 235, 535, 538, 541
BseSI GKGCMC 1 cut(s) 690
BseXI GCAGC 4 cut(s) 159, 1141, 1493, 1561
BseYI CCCAGC 3 cut(s) 414, 538, 1869
BsgI GTGCAG 1 cut(s) 1206
Bsh1236I CGCG 2 cut(s) 1038, 1878
BshFI GGCC 2 cut(s) 424, 937
BshNI GGYRCC 1 cut(s) 1597
BsiSI CCGG 3 cut(s) 323, 1669, 1787
BslFI GGGAC 3 cut(s) 123, 968, 1914
BslI CCNNNNNNNGG 4 cut(s) 141, 420, 796, 1004
BsmFI GGGAC 3 cut(s) 123, 968, 1914
BsmI GAATGC 2 cut(s) 1212, 1577
BsnI GGCC 2 cut(s) 424, 937
Bsp1286I GDGCHC 3 cut(s) 690, 1138, 1307
Bsp13I TCCGGA 1 cut(s) 1668
Bsp143I GATC 6 cut(s) 16, 124, 625, 669, 1258, 1264
Bsp1720I GCTNAGC 1 cut(s) 1937
BspACI CCGC 7 cut(s) 105, 320, 741, 1277, 1615, 1776, 1954
BspANI GGCC 2 cut(s) 424, 937
BspCNI CTCAG 8 cut(s) 15, 172, 439, 643, 919, 1594, 1950, 2015
BspEI TCCGGA 1 cut(s) 1668
BspFNI CGCG 2 cut(s) 1038, 1878
BspHI TCATGA 2 cut(s) 642, 1698
BspLI GGNNCC 2 cut(s) 627, 1599
BspPI GGATC 3 cut(s) 119, 620, 633
BspT107I GGYRCC 1 cut(s) 1597
BspTI CTTAAG 1 cut(s) 1291
BsrBI CCGCTC 2 cut(s) 743, 1617
BsrDI GCAATG 2 cut(s) 782, 1884
BsrFI RCCGGY 1 cut(s) 322
BsrI ACTGG 5 cut(s) 135, 974, 1330, 1426, 1849
BssAI RCCGGY 1 cut(s) 322
BssECI CCNNGG 3 cut(s) 140, 141, 419
BssMI GATC 6 cut(s) 16, 124, 625, 669, 1258, 1264
BssNAI GTATAC 1 cut(s) 1447
BssNI GRCGYC 1 cut(s) 1164
BssSI CACGAG 2 cut(s) 192, 1203
Bst1107I GTATAC 1 cut(s) 1447
Bst2BI CACGAG 2 cut(s) 192, 1203
Bst2UI CCWGG 2 cut(s) 142, 1854
Bst4CI ACNGT 4 cut(s) 92, 986, 1337, 1862
Bst6I CTCTTC 3 cut(s) 615, 1190, 1587
BstACI GRCGYC 1 cut(s) 1164
BstAFI CTTAAG 1 cut(s) 1291
BstC8I GCNNGC 1 cut(s) 1210
BstDEI CTNAG 9 cut(s) 23, 180, 402, 426, 651, 927, 1602, 1937, 2002
BstENI CCTNNNNNAGG 1 cut(s) 794
BstF5I GGATG 6 cut(s) 395, 458, 823, 1619, 1771, 1772
BstFNI CGCG 2 cut(s) 1038, 1878
BstHHI GCGC 2 cut(s) 1495, 1880
BstKTI GATC 6 cut(s) 19, 127, 628, 672, 1261, 1267
BstMBI GATC 6 cut(s) 16, 124, 625, 669, 1258, 1264
BstMWI GCNNNNNNNGC 8 cut(s) 415, 1142, 1151, 1163, 1574, 1775, 1875, 1886
BstNI CCWGG 2 cut(s) 142, 1854
BstNSI RCATGY 1 cut(s) 1359
BstSCI CCNGG 2 cut(s) 140, 1852
BstSFI CTRYAG 1 cut(s) 1945
BstSLI GKGCMC 1 cut(s) 690
BstUI CGCG 2 cut(s) 1038, 1878
BstV1I GCAGC 4 cut(s) 159, 1141, 1493, 1561
BstX2I RGATCY 1 cut(s) 625
BstYI RGATCY 1 cut(s) 625
BstZ17I GTATAC 1 cut(s) 1447
Bsu36I CCTNAGG 3 cut(s) 651, 927, 1602
BsuRI GGCC 2 cut(s) 424, 937
BtsCI GGATG 6 cut(s) 395, 458, 823, 1619, 1771, 1772
BtsIMutI CAGTG 2 cut(s) 981, 1194
Cac8I GCNNGC 1 cut(s) 1210
CaiI CAGNNNCTG 1 cut(s) 1509
CciI TCATGA 2 cut(s) 642, 1698
CfoI GCGC 2 cut(s) 1495, 1880
Cfr10I RCCGGY 1 cut(s) 322
Cfr13I GGNCC 1 cut(s) 1328
CseI GACGC 1 cut(s) 1172
Csp6I GTAC 3 cut(s) 184, 987, 1598
CviAII CATG 6 cut(s) 6, 643, 1142, 1356, 1699, 1718
CviQI GTAC 3 cut(s) 184, 987, 1598
DdeI CTNAG 9 cut(s) 23, 180, 402, 426, 651, 927, 1602, 1937, 2002
DpnI GATC 6 cut(s) 18, 126, 627, 671, 1260, 1266
DpnII GATC 6 cut(s) 16, 124, 625, 669, 1258, 1264
EaeI YGGCCR 1 cut(s) 935
Eam1104I CTCTTC 3 cut(s) 615, 1190, 1587
EarI CTCTTC 3 cut(s) 615, 1190, 1587
Eco24I GRGCYC 2 cut(s) 1138, 1307
Eco32I GATATC 3 cut(s) 658, 1099, 1609
Eco47I GGWCC 1 cut(s) 1328
Eco57I CTGAAG 2 cut(s) 777, 1401
Eco81I CCTNAGG 3 cut(s) 651, 927, 1602
EcoNI CCTNNNNNAGG 1 cut(s) 794
EcoRII CCWGG 2 cut(s) 140, 1852
EcoRV GATATC 3 cut(s) 658, 1099, 1609
EcoT38I GRGCYC 2 cut(s) 1138, 1307
FaeI CATG 6 cut(s) 9, 646, 1145, 1359, 1702, 1721
FalI AAGNNNNNCTT 6 cut(s) 719, 751, 1354, 1386, 1396, 1428
FaqI GGGAC 3 cut(s) 123, 968, 1914
FatI CATG 6 cut(s) 5, 642, 1141, 1355, 1698, 1717
FbaI TGATCA 2 cut(s) 669, 1258
FblI GTMKAC 4 cut(s) 374, 519, 1446, 1553
Fnu4HI GCNGC 6 cut(s) 148, 741, 1155, 1507, 1575, 1954
FokI GGATG 6 cut(s) 382, 445, 830, 1606, 1759, 1778
FriOI GRGCYC 2 cut(s) 1138, 1307
Fsp4HI GCNGC 6 cut(s) 148, 741, 1155, 1507, 1575, 1954
FspBI CTAG 1 cut(s) 1830
GlaI GCGC 2 cut(s) 1494, 1879
GluI GCNGC 6 cut(s) 148, 741, 1155, 1507, 1575, 1954
GsaI CCCAGC 3 cut(s) 418, 542, 1873
GsuI CTGGAG 1 cut(s) 1443
HaeIII GGCC 2 cut(s) 424, 937
HapII CCGG 3 cut(s) 323, 1669, 1787
HgaI GACGC 1 cut(s) 1172
HhaI GCGC 2 cut(s) 1495, 1880
Hin1I GRCGYC 1 cut(s) 1164
Hin1II CATG 6 cut(s) 9, 646, 1145, 1359, 1702, 1721
Hin6I GCGC 2 cut(s) 1493, 1878
HinP1I GCGC 2 cut(s) 1493, 1878
HincII GTYRAC 2 cut(s) 578, 1554
HindII GTYRAC 2 cut(s) 578, 1554
HindIII AAGCTT 2 cut(s) 1253, 1528
HpaII CCGG 3 cut(s) 323, 1669, 1787
HphI GGTGA 5 cut(s) 222, 1299, 1546, 1552, 1693
Hpy166II GTNNAC 7 cut(s) 375, 520, 578, 1447, 1554, 1850, 1865
Hpy8I GTNNAC 7 cut(s) 375, 520, 578, 1447, 1554, 1850, 1865
HpyCH4III ACNGT 4 cut(s) 92, 986, 1337, 1862
HpyCH4V TGCA 8 cut(s) 968, 1145, 1157, 1223, 1355, 1397, 1577, 1889
HpyF10VI GCNNNNNNNGC 8 cut(s) 415, 1142, 1151, 1163, 1574, 1775, 1875, 1886
HpyF3I CTNAG 9 cut(s) 23, 180, 402, 426, 651, 927, 1602, 1937, 2002
Hsp92I GRCGYC 1 cut(s) 1164
Hsp92II CATG 6 cut(s) 9, 646, 1145, 1359, 1702, 1721
HspAI GCGC 2 cut(s) 1493, 1878
Kpn2I TCCGGA 1 cut(s) 1668
KpnI GGTACC 1 cut(s) 1601
Ksp22I TGATCA 2 cut(s) 669, 1258
Kzo9I GATC 6 cut(s) 16, 124, 625, 669, 1258, 1264
LmnI GCTCC 3 cut(s) 513, 772, 1029
Lsp1109I GCAGC 4 cut(s) 159, 1141, 1493, 1561
LweI GCATC 4 cut(s) 17, 299, 1166, 1756
MaeI CTAG 1 cut(s) 1830
MaeIII GTNAC 6 cut(s) 325, 568, 802, 980, 1321, 1840
MalI GATC 6 cut(s) 18, 126, 627, 671, 1260, 1266
MbiI CCGCTC 2 cut(s) 743, 1617
MboI GATC 6 cut(s) 16, 124, 625, 669, 1258, 1264
MfeI CAATTG 1 cut(s) 1656
MflI RGATCY 1 cut(s) 625
MhlI GDGCHC 3 cut(s) 690, 1138, 1307
MluI ACGCGT 1 cut(s) 1036
MlyI GAGTC 5 cut(s) 1193, 1559, 1643, 1896, 1920
MmeI TCCRAC 4 cut(s) 795, 1107, 1261, 1717
MroI TCCGGA 1 cut(s) 1668
MroXI GAANNNNTTC 1 cut(s) 70
MseI TTAA 6 cut(s) 204, 381, 1149, 1292, 1361, 1403
MslI CAYNNNNRTG 1 cut(s) 120
MspA1I CMGCKG 2 cut(s) 107, 1506
MspCI CTTAAG 1 cut(s) 1291
MspI CCGG 3 cut(s) 323, 1669, 1787
MspR9I CCNGG 2 cut(s) 142, 1854
MunI CAATTG 1 cut(s) 1656
Mva1269I GAATGC 2 cut(s) 1212, 1577
MvaI CCWGG 2 cut(s) 142, 1854
MvnI CGCG 2 cut(s) 1038, 1878
MwoI GCNNNNNNNGC 8 cut(s) 415, 1142, 1151, 1163, 1574, 1775, 1875, 1886
NdeII GATC 6 cut(s) 16, 124, 625, 669, 1258, 1264
NlaIII CATG 6 cut(s) 9, 646, 1145, 1359, 1702, 1721
NlaIV GGNNCC 2 cut(s) 627, 1599
NmeAIII GCCGAG 1 cut(s) 400
NmuCI GTSAC 2 cut(s) 325, 802
NspI RCATGY 1 cut(s) 1359
PagI TCATGA 2 cut(s) 642, 1698
PasI CCCWGGG 1 cut(s) 141
PctI GAATGC 2 cut(s) 1212, 1577
PdmI GAANNNNTTC 1 cut(s) 70
PfeI GAWTC 7 cut(s) 646, 700, 917, 1297, 1346, 1480, 1543
PflFI GACNNNGTC 1 cut(s) 959
PkrI GCNGC 6 cut(s) 149, 742, 1156, 1508, 1576, 1955
PleI GAGTC 5 cut(s) 1193, 1558, 1642, 1896, 1920
PpsI GAGTC 5 cut(s) 1193, 1558, 1642, 1896, 1920
PsiI TTATAA 3 cut(s) 252, 834, 861
Psp6I CCWGG 2 cut(s) 140, 1852
PspFI CCCAGC 3 cut(s) 414, 538, 1869
PspGI CCWGG 2 cut(s) 140, 1852
PspN4I GGNNCC 2 cut(s) 627, 1599
PspPI GGNCC 1 cut(s) 1328
PstNI CAGNNNCTG 1 cut(s) 1509
PsuI RGATCY 1 cut(s) 625
PsyI GACNNNGTC 1 cut(s) 959
PvuII CAGCTG 1 cut(s) 1506
RsaI GTAC 3 cut(s) 185, 988, 1599
RsaNI GTAC 3 cut(s) 184, 987, 1598
RseI CAYNNNNRTG 1 cut(s) 120
SalI GTCGAC 1 cut(s) 1552
SaqAI TTAA 6 cut(s) 204, 381, 1149, 1292, 1361, 1403
SatI GCNGC 6 cut(s) 148, 741, 1155, 1507, 1575, 1954
Sau3AI GATC 6 cut(s) 16, 124, 625, 669, 1258, 1264
Sau96I GGNCC 1 cut(s) 1328
ScaI AGTACT 2 cut(s) 185, 988
SchI GAGTC 5 cut(s) 1193, 1559, 1643, 1896, 1920
ScrFI CCNGG 2 cut(s) 142, 1854
SduI GDGCHC 3 cut(s) 690, 1138, 1307
SfaNI GCATC 4 cut(s) 17, 299, 1166, 1756
SfcI CTRYAG 1 cut(s) 1945
SinI GGWCC 1 cut(s) 1328
SmiMI CAYNNNNRTG 1 cut(s) 120
SmlI CTYRAG 2 cut(s) 1291, 1904
SmoI CTYRAG 2 cut(s) 1291, 1904
SpeI ACTAGT 1 cut(s) 1829
SsiI CCGC 7 cut(s) 105, 320, 741, 1277, 1615, 1776, 1954
SspI AATATT 1 cut(s) 299
SspMI CTAG 1 cut(s) 1830
StyD4I CCNGG 2 cut(s) 140, 1852
TaaI ACNGT 4 cut(s) 92, 986, 1337, 1862
TaqI TCGA 4 cut(s) 64, 1553, 1691, 1711
TatI WGTACW 2 cut(s) 183, 986
TauI GCSGC 2 cut(s) 743, 1956
TfiI GAWTC 7 cut(s) 646, 700, 917, 1297, 1346, 1480, 1543
Tru1I TTAA 6 cut(s) 204, 381, 1149, 1292, 1361, 1403
Tru9I TTAA 6 cut(s) 204, 381, 1149, 1292, 1361, 1403
TscAI CASTG 2 cut(s) 981, 1201
TseFI GTSAC 2 cut(s) 325, 802
TseI GCWGC 4 cut(s) 147, 1154, 1506, 1574
Tsp45I GTSAC 2 cut(s) 325, 802
TspGWI ACGGA 1 cut(s) 1571
TspRI CASTG 2 cut(s) 981, 1201
Tth111I GACNNNGTC 1 cut(s) 959
Vha464I CTTAAG 1 cut(s) 1291
VpaK11BI GGWCC 1 cut(s) 1328
XagI CCTNNNNNAGG 1 cut(s) 794
XapI RAATTY 2 cut(s) 837, 1049
XceI RCATGY 1 cut(s) 1359
XmiI GTMKAC 4 cut(s) 374, 519, 1446, 1553
XmnI GAANNNNTTC 1 cut(s) 70
XspI CTAG 1 cut(s) 1830
ZrmI AGTACT 2 cut(s) 185, 988
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.