Rh4CG096300

RNA helicase SDE3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0000595
Physical Location & Seq
Forward (+)
17318056 .. 17335847
17792 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG096300.1

Sequence Viewer

Length: 567 bp
ATGTCGGCTTATCTCAAATTCTTTAAGCCGATTCTATCGCCCACCTTGGGGAAGAAGAATTATGTATGGGTTGAGAAAGATTCATTACCTATATTCACAATTCCTGAGAATGTCAAAGATTTGATCAAGAACGACATTGTGCCCAAGAAATGGAGTGATTACCATTTGGAGAACATGGTATTGGAGTTGCGAGAAGAAGAAATTTATAATTATAAAACTCAAGAAAAGGAAAGAAAAACTTTTGTTGCATTTGCGATTGGTTCTGTTCCTGAGAGACGACCTTTCCTCTTATCGAGGGACCTGGTGTATGTACGACCATCAGATAGGGATACTGAGCACTTCCAGATGAAGACAGGGATAACTATGTGGATGTCCCTTAAGCAGCTTGAGAGACCGGCTGATAAGGGCTCACCACCTTACCTAGTAGCCCGCGAACTCTGTGTTGAATTGAAAACCTTTCCGAACCATCGAGGACTGGACTGGCTATTTGTGAAGCAATACTCCAGGTGTTGTGATGTGCTTCTGAAAAGCTTGATGGAAGTGATTCCAGAGTCGGATATGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

188

Amino Acids

22.27

Weight (kDa)

8.32

Isoelectric Point (pI)

44.6

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000595)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g37100 FvH4_4g37100 FvH4_4g37100
malus_domestica MD13G1238300.v1.1 MD16G1243100.v1.1 MD16G1243200.v1.1
prunus_persica Prupe.1G070100_v2.0.a1 Prupe.1G070200_v2.0.a1 Prupe.1G070300_v2.0.a1 Prupe.1G070300_v2.0.a1
pyrus_communis pycom13g21070 pycom16g20390 pycom16g20400
rosa_chinensis RchiOBHm_Chr1g0318071 RchiOBHm_Chr1g0318111 RchiOBHm_Chr4g0446861 RchiOBHm_Chr4g0446881 RchiOBHm_Chr4g0446891
rosa_laevigata RLG00000005623 RLG00000005624 RLG00000009337 RLG00000030560 RLG00000030566
rosa_multiflora Rmu_co8177450.1_g000001 Rmu_sc0001753.1_g000012 Rmu_sc0001753.1_g000013 Rmu_sc0001753.1_g000014 Rmu_sc0001753.1_g000015 Rmu_sc0011424.1_g000009 Rmu_sc0011424.1_g000020 Rmu_sc0034555.1_g000001 Rmu_ssc0000008.1_g000002
rosa_roxburghii Rroxscaffold_4G00329530 Rroxscaffold_4G00329590 Rroxscaffold_4G00329800 Rroxscaffold_4G00329810 Rroxscaffold_4G00329860 Rroxscaffold_4G00329910 Rroxscaffold_4G00330030 Rroxscaffold_5G00343610 Rroxscaffold_5G00348280 Rroxscaffold_5G00387220 Rroxscaffold_5G00387230
rosa_rugosa Rorug01G0019100 Rorug01G0019100 Rorug01G0019500 Rorug01G0019600 Rorug04G0010100 Rorug04G0375200 Rorug04G0375300 Rorug04G0375400 Rorug04G0375500 Rorug04G0375600 Rorug04G0375700
rosa_samantha Rh1AG030700 Rh1AG030900 Rh1BG026900 Rh1CG029500 Rh1CG029700 Rh1DG040500 Rh4AG088100 Rh4AG436700 Rh4AG436800 Rh4AG436900 Rh4BG084700 Rh4BG445800 Rh4BG445900 Rh4BG446100 Rh4CG096300 Rh4CG463100 Rh4CG463200 Rh4CG463400 Rh4DG444500 Rh4DG444600 Rh4DG444700
rosa_wichuraiana Rw1G002420 Rw1G002440 Rw1G002480 Rw4G037360 Rw4G037370 Rw4G037380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 207, 213
AccB7I CCANNNNNTGG 1 cut(s) 150
AccII CGCG 1 cut(s) 432
AciI CCGC 1 cut(s) 430
AcsI RAATTY 2 cut(s) 17, 201
AfaI GTAC 1 cut(s) 312
AfiI CCNNNNNNNGG 3 cut(s) 47, 48, 150
AflII CTTAAG 1 cut(s) 377
AgsI TTSAA 2 cut(s) 446, 451
AjnI CCWGG 2 cut(s) 300, 503
AjuI GAANNNNNNNTTGG 2 cut(s) 164, 196
AluBI AGCT 2 cut(s) 385, 531
AluI AGCT 2 cut(s) 385, 531
Alw21I GWGCWC 1 cut(s) 339
Alw26I GTCTC 2 cut(s) 268, 385
ApeKI GCWGC 1 cut(s) 382
ApoI RAATTY 2 cut(s) 17, 201
ArsI GACNNNNNNTTYG 2 cut(s) 470, 502
Asp700I GAANNNNTTC 1 cut(s) 543
AspS9I GGNCC 1 cut(s) 298
AsuHPI GGTGA 1 cut(s) 402
AvaII GGWCC 1 cut(s) 298
BaeGI GKGCMC 1 cut(s) 144
BanII GRGCYC 1 cut(s) 410
BbsI GAAGAC 1 cut(s) 356
Bbv12I GWGCWC 1 cut(s) 339
BbvI GCAGC 1 cut(s) 394
BccI CCATC 3 cut(s) 325, 474, 529
BciT130I CCWGG 2 cut(s) 302, 505
BciVI GTATCC 1 cut(s) 322
BclI TGATCA 1 cut(s) 123
BcoDI GTCTC 2 cut(s) 268, 385
BfaI CTAG 1 cut(s) 422
BfrI CTTAAG 1 cut(s) 377
BfuI GTATCC 1 cut(s) 322
BisI GCNGC 1 cut(s) 383
BlsI GCNGC 1 cut(s) 384
Bme1390I CCNGG 2 cut(s) 302, 505
Bme18I GGWCC 1 cut(s) 298
BmgT120I GGNCC 1 cut(s) 298
BmiI GGNNCC 1 cut(s) 299
BmrFI CCNGG 2 cut(s) 302, 505
BpiI GAAGAC 1 cut(s) 356
BpmI CTGGAG 1 cut(s) 487
BpuEI CTTGAG 2 cut(s) 204, 407
BsaI GGTCTC 1 cut(s) 385
BsaJI CCNNGG 1 cut(s) 45
Bsc4I CCNNNNNNNGG 3 cut(s) 47, 48, 150
Bse118I RCCGGY 1 cut(s) 394
Bse1I ACTGG 2 cut(s) 480, 485
BseBI CCWGG 2 cut(s) 302, 505
BseDI CCNNGG 1 cut(s) 45
BseGI GGATG 1 cut(s) 375
BseLI CCNNNNNNNGG 3 cut(s) 47, 48, 150
BseMII CTCAG 3 cut(s) 96, 261, 324
BseNI ACTGG 2 cut(s) 480, 485
BseSI GKGCMC 1 cut(s) 144
BseXI GCAGC 1 cut(s) 394
Bsh1236I CGCG 1 cut(s) 432
BsiHKAI GWGCWC 1 cut(s) 339
BsiSI CCGG 1 cut(s) 395
BslFI GGGAC 2 cut(s) 311, 358
BslI CCNNNNNNNGG 3 cut(s) 47, 48, 150
BsmAI GTCTC 2 cut(s) 268, 385
BsmBI CGTCTC 1 cut(s) 268
BsmFI GGGAC 2 cut(s) 311, 358
Bso31I GGTCTC 1 cut(s) 385
Bsp1286I GDGCHC 3 cut(s) 144, 339, 410
Bsp143I GATC 1 cut(s) 123
BspACI CCGC 1 cut(s) 430
BspCNI CTCAG 3 cut(s) 97, 262, 325
BspFNI CGCG 1 cut(s) 432
BspLI GGNNCC 1 cut(s) 299
BspTI CTTAAG 1 cut(s) 377
BspTNI GGTCTC 1 cut(s) 385
BsrFI RCCGGY 1 cut(s) 394
BsrI ACTGG 2 cut(s) 480, 485
BssAI RCCGGY 1 cut(s) 394
BssECI CCNNGG 1 cut(s) 45
BssMI GATC 1 cut(s) 123
BssT1I CCWWGG 1 cut(s) 45
Bst2UI CCWGG 2 cut(s) 302, 505
BstAFI CTTAAG 1 cut(s) 377
BstC8I GCNNGC 1 cut(s) 430
BstDEI CTNAG 3 cut(s) 105, 270, 333
BstF5I GGATG 1 cut(s) 375
BstFNI CGCG 1 cut(s) 432
BstKTI GATC 1 cut(s) 126
BstMAI GTCTC 2 cut(s) 268, 385
BstMBI GATC 1 cut(s) 123
BstNI CCWGG 2 cut(s) 302, 505
BstSCI CCNGG 2 cut(s) 300, 503
BstSLI GKGCMC 1 cut(s) 144
BstUI CGCG 1 cut(s) 432
BstV1I GCAGC 1 cut(s) 394
BstV2I GAAGAC 1 cut(s) 356
BsuI GTATCC 1 cut(s) 322
BtsCI GGATG 1 cut(s) 375
Cac8I GCNNGC 1 cut(s) 430
Cfr10I RCCGGY 1 cut(s) 394
Cfr13I GGNCC 1 cut(s) 298
CsiI ACCWGGT 1 cut(s) 300
Csp6I GTAC 1 cut(s) 311
CviAII CATG 1 cut(s) 175
CviJI RGCY 8 cut(s) 8, 28, 385, 398, 408, 428, 484, 531
CviKI_1 RGCY 8 cut(s) 8, 28, 385, 398, 408, 428, 484, 531
CviQI GTAC 1 cut(s) 311
DdeI CTNAG 3 cut(s) 105, 270, 333
DpnI GATC 1 cut(s) 125
DpnII GATC 1 cut(s) 123
Eco130I CCWWGG 1 cut(s) 45
Eco24I GRGCYC 1 cut(s) 410
Eco31I GGTCTC 1 cut(s) 385
Eco47I GGWCC 1 cut(s) 298
EcoO109I RGGNCCY 1 cut(s) 298
EcoRII CCWGG 2 cut(s) 300, 503
EcoT14I CCWWGG 1 cut(s) 45
EcoT38I GRGCYC 1 cut(s) 410
ErhI CCWWGG 1 cut(s) 45
Esp3I CGTCTC 1 cut(s) 268
FaeI CATG 1 cut(s) 178
FaiI YATR 9 cut(s) 63, 67, 92, 176, 207, 213, 309, 365, 560
FalI AAGNNNNNCTT 2 cut(s) 223, 255
FaqI GGGAC 2 cut(s) 311, 358
FatI CATG 1 cut(s) 174
FauI CCCGC 1 cut(s) 437
FbaI TGATCA 1 cut(s) 123
Fnu4HI GCNGC 1 cut(s) 383
FokI GGATG 1 cut(s) 382
FriOI GRGCYC 1 cut(s) 410
Fsp4HI GCNGC 1 cut(s) 383
FspBI CTAG 1 cut(s) 422
GluI GCNGC 1 cut(s) 383
GsuI CTGGAG 1 cut(s) 487
HapII CCGG 1 cut(s) 395
Hin1II CATG 1 cut(s) 178
HindIII AAGCTT 1 cut(s) 529
HinfI GANTC 4 cut(s) 31, 80, 544, 551
HpaII CCGG 1 cut(s) 395
HphI GGTGA 1 cut(s) 402
Hpy188I TCNGA 4 cut(s) 322, 462, 525, 556
Hpy188III TCNNGA 6 cut(s) 104, 127, 221, 269, 343, 548
HpyCH4V TGCA 1 cut(s) 248
HpyF3I CTNAG 3 cut(s) 105, 270, 333
Hsp92II CATG 1 cut(s) 178
Ksp22I TGATCA 1 cut(s) 123
Kzo9I GATC 1 cut(s) 123
Lsp1109I GCAGC 1 cut(s) 394
MabI ACCWGGT 1 cut(s) 300
MaeI CTAG 1 cut(s) 422
MalI GATC 1 cut(s) 125
MboI GATC 1 cut(s) 123
MboII GAAGA 5 cut(s) 64, 67, 206, 209, 361
MhlI GDGCHC 3 cut(s) 144, 339, 410
MluCI AATT 6 cut(s) 17, 58, 99, 201, 208, 446
MlyI GAGTC 1 cut(s) 560
MmeI TCCRAC 1 cut(s) 534
MnlI CCTC 3 cut(s) 288, 296, 464
MroXI GAANNNNTTC 1 cut(s) 543
MseI TTAA 2 cut(s) 24, 378
MspCI CTTAAG 1 cut(s) 377
MspI CCGG 1 cut(s) 395
MspR9I CCNGG 2 cut(s) 302, 505
MvaI CCWGG 2 cut(s) 302, 505
MvnI CGCG 1 cut(s) 432
NdeII GATC 1 cut(s) 123
NlaIII CATG 1 cut(s) 178
NlaIV GGNNCC 1 cut(s) 299
PdmI GAANNNNTTC 1 cut(s) 543
PfeI GAWTC 3 cut(s) 31, 80, 544
PflMI CCANNNNNTGG 1 cut(s) 150
PkrI GCNGC 1 cut(s) 384
PleI GAGTC 1 cut(s) 559
PpsI GAGTC 1 cut(s) 559
PpuMI RGGWCCY 1 cut(s) 298
PsiI TTATAA 2 cut(s) 207, 213
Psp5II RGGWCCY 1 cut(s) 298
Psp6I CCWGG 2 cut(s) 300, 503
PspGI CCWGG 2 cut(s) 300, 503
PspN4I GGNNCC 1 cut(s) 299
PspPI GGNCC 1 cut(s) 298
PspPPI RGGWCCY 1 cut(s) 298
RsaI GTAC 1 cut(s) 312
RsaNI GTAC 1 cut(s) 311
SaqAI TTAA 2 cut(s) 24, 378
SatI GCNGC 1 cut(s) 383
Sau3AI GATC 1 cut(s) 123
Sau96I GGNCC 1 cut(s) 298
SchI GAGTC 1 cut(s) 560
ScrFI CCNGG 2 cut(s) 302, 505
SduI GDGCHC 3 cut(s) 144, 339, 410
SexAI ACCWGGT 1 cut(s) 300
SinI GGWCC 1 cut(s) 298
SmlI CTYRAG 3 cut(s) 219, 377, 386
SmoI CTYRAG 3 cut(s) 219, 377, 386
Sse9I AATT 6 cut(s) 17, 58, 99, 201, 208, 446
SsiI CCGC 1 cut(s) 430
SspMI CTAG 1 cut(s) 422
StyD4I CCNGG 2 cut(s) 300, 503
StyI CCWWGG 1 cut(s) 45
TaqI TCGA 2 cut(s) 293, 469
TasI AATT 6 cut(s) 17, 58, 99, 201, 208, 446
TfiI GAWTC 3 cut(s) 31, 80, 544
Tru1I TTAA 2 cut(s) 24, 378
Tru9I TTAA 2 cut(s) 24, 378
TseI GCWGC 1 cut(s) 382
TspDTI ATGAA 2 cut(s) 72, 362
Van91I CCANNNNNTGG 1 cut(s) 150
Vha464I CTTAAG 1 cut(s) 377
VpaK11BI GGWCC 1 cut(s) 298
XapI RAATTY 2 cut(s) 17, 201
XmnI GAANNNNTTC 1 cut(s) 543
XspI CTAG 1 cut(s) 422
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.