Rh4DG444600

RNA helicase SDE3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0000595
Physical Location & Seq
Reverse (-)
64492522 .. 64496084
3563 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG444600.1

Sequence Viewer

Length: 2253 bp
ATGAGGTATCGGTTGCTGCTGGTCATCGGGAGTTCAGCAATATTATGGTGGACATACAAGAATCGCAAACCTCCCCCCCCAAGAAACAAATGGTCTCGCTTTATACCATTTTCATCATCCCCTCAGCCTCGTCCTGATGGGGTTTGGCTTAATCGGTATTGGTTGCTGCTAGTCATCGGGAGTTCAGCAATATTATGGTTGACAAACAAGAATCGCAAACCTCGCACCCCCAGAAACGAAAGGTCTCGCTTTATACCATTTTCATCATCCCCTCAGCCTCGTCCTGATGGGGTTTGGCTTAATAGGTATCGGTTGCTGCTGGTCATCGGGAGTTCAGCAATATTATGGTTGACAAACAAGAATCGCAAACCTCCCCCCCCAAGAAACAAATGGTCTCGCTTTATACCATTTTCATCATCCCCTCAGCCTCGTCCTGATGGGGTTTGGCTTAATCGGTATTGGTTGCTGCTAGTCATCGGGAGTTCAGCAATATTATGGTTGACAAACAAGAATCGCAAACCTCGCCCCCCTCCAGAAACGAAAGGGGTCGCTTCCCTGAAAAGCCACCTCAGCGTCAGCCAACATCATCTATATCCAGCCTCACCGAAAACCCCAACATATTTTTCTGGACCATCTCCATCTTCCCCAAAACACCCTCCAACATATTTTTCTACACCATCTCCGTCTCCATCTTCCCTGAAACCCCCAGCATATACTTCTAAACCATCTTCATCCTCAACTCCATCTTCCCCAAAACCCGCCCCCAGCATATATTTTTCTCTATCATCTCCGTCTCCATCTTCCCCAAAGCGCCCTCCTGTCTTTAAGTCAGTTCTATGCCCAGCCTCCTCCTTCTCAAATAATGATGATATAATTCAACAACAACGAGACAGGAAGAAAACAGCTTACGTATGTGTTGAAGAGGATTCATTACCTGTATTCACGATTCCTGAGGATATCAAAACCTTGATCAAGAACGAAACTGTGCCCAAAGTTCTCAACTGCTCTTTGTCTCCCACAACGTACAAGGATTACTTTGCTGCTCTGTTATATTCTGAAGATTTCTTCTTGGAGAAATGGAGTGATTTCCTTTTGAAGGCTGTGACATTGGAGTTGAATGAAGCTGAAATTCATAAAAATCAGAAAAAGCAAGATAAAGCCTTTGTGGCATTTGAGCTTGATTCTGTTCCCCAGAATAGGCCATTCCTCTTATCAAGGGACTTGGTCTTTGCACGACCAGTGGGTAGGAGTACTGCTGAGCCCTTTCAGGGTATCATTAATCGCATTGTAAGGAGCAAAACCGTTTTAGTTGAATTTGAAGATGCCTTTTATACTCATCATCATTCCAACAAAAAGTATGATGTCAGCTTCTCATTCAATAGAGTTTGCTTGAAAAGAGCTCACCAAGCAGTTAAAGATGCATCAGAGGACTTGTTTCAGAATTTCCTCTTCCCTCATTGTGACTCACGAGGTAGCATTCCTACTGCACCAGCTCTGCTTTCTACTACCTGTCATAAGCTCGATCGAGATCAACGTTCTGCAGTTGGACATATCTTAAGGATTCAGGGCTCACCTCCTTATGTAGTAACTGGTCCGGACTCTGTACCTGAAGGCAAATCTTTTGCATATTTTAGAGAACCTTCAAGAACTGGAGTAGTTGTTAGTGAAGCAGTATACCAATTATGTCAGAAGTCGCCAGAGTATCGCATTCTTATCTGTGCGCCCACTAACAGCTGCTGCGATGTGCTGATGAGAAGCTTGGGGAAGGTGATTCCAGAGTCGACTATGTTTCGTGCCAATGCTGCATTCCGTGAGAAAGAAGAGGTACCTGAGGATATCCTCCGCTCATCCCTTTACAAAGAGTCCTGTTTTTCTTGTCCTCCAATAGAGGAACTCCGGAAATACAGGGTGATTTTTTCGACTTTCATGAGTAGCTTTCGACTACATGATAAAGGCATAGCTGTTGGACATTTTAGCCATATTTTTCTGGTGGATGCTTCATCCGCTATTGAACCGGAAACATTGGTCGCCCTGACTAACTTTGCTGACAAGACTACTAGTGTTATAGTTACTGGTAAACCTGGAAACAGTCCGCGCTGGGTTCGCGCTGACATTGCAAGGAAAAAGGGACTCAAGATTTCATACTTTGAAAGACTCTGTAAGCTCTGGCCCTATAGAAGCCTCAGTCCAGTGTTCATCACACAATTGGACCTGAACCAAAACTCTGGCTCAGACTACCAAACAGCTTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

750

Amino Acids

85.04

Weight (kDa)

9.79

Isoelectric Point (pI)

61.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MOV-10_beta-barrel PF21634 380 - 457 8.4e-10 Helicase MOV-10, beta-barrel domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000595)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g37100 FvH4_4g37100 FvH4_4g37100
malus_domestica MD13G1238300.v1.1 MD16G1243100.v1.1 MD16G1243200.v1.1
prunus_persica Prupe.1G070100_v2.0.a1 Prupe.1G070200_v2.0.a1 Prupe.1G070300_v2.0.a1 Prupe.1G070300_v2.0.a1
pyrus_communis pycom13g21070 pycom16g20390 pycom16g20400
rosa_chinensis RchiOBHm_Chr1g0318071 RchiOBHm_Chr1g0318111 RchiOBHm_Chr4g0446861 RchiOBHm_Chr4g0446881 RchiOBHm_Chr4g0446891
rosa_laevigata RLG00000005623 RLG00000005624 RLG00000009337 RLG00000030560 RLG00000030566
rosa_multiflora Rmu_co8177450.1_g000001 Rmu_sc0001753.1_g000012 Rmu_sc0001753.1_g000013 Rmu_sc0001753.1_g000014 Rmu_sc0001753.1_g000015 Rmu_sc0011424.1_g000009 Rmu_sc0011424.1_g000020 Rmu_sc0034555.1_g000001 Rmu_ssc0000008.1_g000002
rosa_roxburghii Rroxscaffold_4G00329530 Rroxscaffold_4G00329590 Rroxscaffold_4G00329800 Rroxscaffold_4G00329810 Rroxscaffold_4G00329860 Rroxscaffold_4G00329910 Rroxscaffold_4G00330030 Rroxscaffold_5G00343610 Rroxscaffold_5G00348280 Rroxscaffold_5G00387220 Rroxscaffold_5G00387230
rosa_rugosa Rorug01G0019100 Rorug01G0019100 Rorug01G0019500 Rorug01G0019600 Rorug04G0010100 Rorug04G0375200 Rorug04G0375300 Rorug04G0375400 Rorug04G0375500 Rorug04G0375600 Rorug04G0375700
rosa_samantha Rh1AG030700 Rh1AG030900 Rh1BG026900 Rh1CG029500 Rh1CG029700 Rh1DG040500 Rh4AG088100 Rh4AG436700 Rh4AG436800 Rh4AG436900 Rh4BG084700 Rh4BG445800 Rh4BG445900 Rh4BG446100 Rh4CG096300 Rh4CG463100 Rh4CG463200 Rh4CG463400 Rh4DG444500 Rh4DG444600 Rh4DG444700
rosa_wichuraiana Rw1G002420 Rw1G002440 Rw1G002480 Rw4G037360 Rw4G037370 Rw4G037380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1825
AccB1I GGYRCC 1 cut(s) 1825
AccBSI CCGCTC 1 cut(s) 1845
AccI GTMKAC 2 cut(s) 1674, 1781
AccII CGCG 2 cut(s) 2095, 2106
AccIII TCCGGA 2 cut(s) 1594, 1896
AciI CCGC 4 cut(s) 759, 1843, 2004, 2093
AclI AACGTT 1 cut(s) 1534
AcsI RAATTY 3 cut(s) 1128, 1313, 1441
AcuI CTGAAG 2 cut(s) 1077, 1629
AfaI GTAC 4 cut(s) 1025, 1252, 1605, 1827
AfiI CCNNNNNNNGG 3 cut(s) 1096, 1197, 1268
AflII CTTAAG 1 cut(s) 1555
AhlI ACTAGT 1 cut(s) 2057
AjnI CCWGG 1 cut(s) 2080
AjuI GAANNNNNNNTTGG 6 cut(s) 607, 639, 652, 684, 1790, 1822
Alw21I GWGCWC 1 cut(s) 1402
Alw26I GTCTC 7 cut(s) 99, 249, 399, 690, 798, 882, 1017
AlwNI CAGNNNCTG 1 cut(s) 1737
Aor13HI TCCGGA 2 cut(s) 1594, 1896
AoxI GGCC 2 cut(s) 1199, 2168
ApeKI GCWGC 8 cut(s) 16, 166, 316, 466, 1040, 1734, 1737, 1802
ApoI RAATTY 3 cut(s) 1128, 1313, 1441
ArsI GACNNNNNNTTYG 2 cut(s) 1211, 1243
AseI ATTAAT 1 cut(s) 1278
Asp718I GGTACC 1 cut(s) 1825
AspLEI GCGC 4 cut(s) 813, 1723, 2097, 2108
AspS9I GGNCC 4 cut(s) 629, 1592, 2169, 2209
AsuHPI GGTGA 5 cut(s) 594, 1394, 1563, 1780, 1921
AvaII GGWCC 3 cut(s) 629, 1592, 2209
AxyI CCTNAGG 2 cut(s) 951, 1830
BaeGI GKGCMC 1 cut(s) 990
BanI GGYRCC 1 cut(s) 1825
BanII GRGCYC 3 cut(s) 1263, 1402, 1571
BauI CACGAG 1 cut(s) 1467
Bbv12I GWGCWC 1 cut(s) 1402
BbvCI CCTCAGC 4 cut(s) 123, 273, 423, 569
BbvI GCAGC 8 cut(s) 3, 153, 303, 453, 1027, 1721, 1724, 1789
BciT130I CCWGG 1 cut(s) 2082
BclI TGATCA 1 cut(s) 969
BcoDI GTCTC 7 cut(s) 99, 249, 399, 690, 798, 882, 1017
BcuI ACTAGT 1 cut(s) 2057
BfaI CTAG 3 cut(s) 170, 470, 2058
BfmI CTRYAG 2 cut(s) 1539, 2173
BfoI RGCGCY 1 cut(s) 814
BfrI CTTAAG 1 cut(s) 1555
BglI GCCNNNNNGGC 1 cut(s) 1166
BisI GCNGC 8 cut(s) 17, 167, 317, 467, 1041, 1735, 1738, 1803
BlpI GCTNAGC 1 cut(s) 1257
BlsI GCNGC 8 cut(s) 18, 168, 318, 468, 1042, 1736, 1739, 1804
BmcAI AGTACT 1 cut(s) 1252
Bme1390I CCNGG 1 cut(s) 2082
Bme18I GGWCC 3 cut(s) 629, 1592, 2209
BmgT120I GGNCC 4 cut(s) 629, 1592, 2169, 2209
BmiI GGNNCC 1 cut(s) 1827
BmrFI CCNGG 1 cut(s) 2082
BmsI GCATC 4 cut(s) 1312, 1408, 1430, 1984
BpmI CTGGAG 2 cut(s) 516, 1671
Bpu10I CCTNAGC 4 cut(s) 123, 273, 423, 569
Bpu1102I GCTNAGC 1 cut(s) 1257
BpuEI CTTGAG 1 cut(s) 2117
BsaAI YACGTR 1 cut(s) 910
BsaBI GATNNNNATC 1 cut(s) 1527
BsaI GGTCTC 3 cut(s) 99, 249, 399
BsaWI WCCGGW 3 cut(s) 1594, 1896, 2014
BsaXI ACNNNNNCTCC 4 cut(s) 664, 694, 1644, 1674
Bsc4I CCNNNNNNNGG 3 cut(s) 1096, 1197, 1268
Bse1I ACTGG 5 cut(s) 1238, 1594, 1654, 2077, 2189
Bse21I CCTNAGG 2 cut(s) 951, 1830
Bse3DI GCAATG 1 cut(s) 2112
Bse8I GATNNNNATC 1 cut(s) 1527
BseAI TCCGGA 2 cut(s) 1594, 1896
BseBI CCWGG 1 cut(s) 2082
BseGI GGATG 7 cut(s) 116, 266, 416, 731, 1847, 1999, 2000
BseJI GATNNNNATC 1 cut(s) 1527
BseLI CCNNNNNNNGG 3 cut(s) 1096, 1197, 1268
BseMI GCAATG 1 cut(s) 2112
BseMII CTCAG 9 cut(s) 137, 287, 437, 583, 942, 1248, 1821, 2197, 2244
BseNI ACTGG 5 cut(s) 1238, 1594, 1654, 2077, 2189
BseRI GAGGAG 1 cut(s) 838
BseSI GKGCMC 1 cut(s) 990
BseXI GCAGC 8 cut(s) 3, 153, 303, 453, 1027, 1721, 1724, 1789
BseYI CCCAGC 4 cut(s) 706, 764, 841, 2097
BsgI GTGCAG 1 cut(s) 1470
Bsh1236I CGCG 2 cut(s) 2095, 2106
Bsh1285I CGRYCG 1 cut(s) 1525
BshFI GGCC 2 cut(s) 1201, 2170
BshNI GGYRCC 1 cut(s) 1825
BsiEI CGRYCG 1 cut(s) 1525
BsiHKAI GWGCWC 1 cut(s) 1402
BsiSI CCGG 3 cut(s) 1595, 1897, 2015
BslFI GGGAC 2 cut(s) 1232, 2142
BslI CCNNNNNNNGG 3 cut(s) 1096, 1197, 1268
BsmAI GTCTC 7 cut(s) 99, 249, 399, 690, 798, 882, 1017
BsmBI CGTCTC 2 cut(s) 690, 798
BsmFI GGGAC 2 cut(s) 1232, 2142
BsmI GAATGC 3 cut(s) 1476, 1707, 1805
BsnI GGCC 2 cut(s) 1201, 2170
Bso31I GGTCTC 3 cut(s) 99, 249, 399
Bsp1286I GDGCHC 4 cut(s) 990, 1263, 1402, 1571
Bsp13I TCCGGA 2 cut(s) 1594, 1896
Bsp143I GATC 3 cut(s) 969, 1522, 1528
Bsp1720I GCTNAGC 1 cut(s) 1257
BspACI CCGC 4 cut(s) 759, 1843, 2004, 2093
BspANI GGCC 2 cut(s) 1201, 2170
BspCNI CTCAG 9 cut(s) 136, 286, 436, 582, 943, 1249, 1822, 2196, 2243
BspEI TCCGGA 2 cut(s) 1594, 1896
BspFNI CGCG 2 cut(s) 2095, 2106
BspHI TCATGA 1 cut(s) 1926
BspLI GGNNCC 1 cut(s) 1827
BspMAI CTGCAG 1 cut(s) 1543
BspT107I GGYRCC 1 cut(s) 1825
BspTI CTTAAG 1 cut(s) 1555
BspTNI GGTCTC 3 cut(s) 99, 249, 399
BsrBI CCGCTC 1 cut(s) 1845
BsrDI GCAATG 1 cut(s) 2112
BsrI ACTGG 5 cut(s) 1238, 1594, 1654, 2077, 2189
BssMI GATC 3 cut(s) 969, 1522, 1528
BssNAI GTATAC 1 cut(s) 1675
BssSI CACGAG 1 cut(s) 1467
Bst1107I GTATAC 1 cut(s) 1675
Bst2BI CACGAG 1 cut(s) 1467
Bst2UI CCWGG 1 cut(s) 2082
Bst4CI ACNGT 3 cut(s) 985, 1303, 2090
Bst6I CTCTTC 3 cut(s) 915, 1454, 1815
BstAFI CTTAAG 1 cut(s) 1555
BstBAI YACGTR 1 cut(s) 910
BstDEI CTNAG 9 cut(s) 123, 273, 423, 569, 951, 1257, 1830, 2183, 2230
BstENI CCTNNNNNAGG 1 cut(s) 1094
BstF5I GGATG 7 cut(s) 116, 266, 416, 731, 1847, 1999, 2000
BstFNI CGCG 2 cut(s) 2095, 2106
BstH2I RGCGCY 1 cut(s) 814
BstHHI GCGC 4 cut(s) 813, 1723, 2097, 2108
BstKTI GATC 3 cut(s) 972, 1525, 1531
BstMAI GTCTC 7 cut(s) 99, 249, 399, 690, 798, 882, 1017
BstMBI GATC 3 cut(s) 969, 1522, 1528
BstMCI CGRYCG 1 cut(s) 1525
BstMWI GCNNNNNNNGC 9 cut(s) 222, 522, 570, 1166, 1406, 1802, 2003, 2103, 2114
BstNI CCWGG 1 cut(s) 2082
BstSCI CCNGG 1 cut(s) 2080
BstSFI CTRYAG 2 cut(s) 1539, 2173
BstSLI GKGCMC 1 cut(s) 990
BstSNI TACGTA 1 cut(s) 910
BstUI CGCG 2 cut(s) 2095, 2106
BstV1I GCAGC 8 cut(s) 3, 153, 303, 453, 1027, 1721, 1724, 1789
BstXI CCANNNNNNTGG 1 cut(s) 2225
BstZ17I GTATAC 1 cut(s) 1675
Bsu36I CCTNAGG 2 cut(s) 951, 1830
BsuRI GGCC 2 cut(s) 1201, 2170
BtgZI GCGATG 1 cut(s) 1755
BtsCI GGATG 7 cut(s) 116, 266, 416, 731, 1847, 1999, 2000
BtsIMutI CAGTG 2 cut(s) 1245, 2196
CaiI CAGNNNCTG 1 cut(s) 1737
CciI TCATGA 1 cut(s) 1926
CfoI GCGC 4 cut(s) 813, 1723, 2097, 2108
Cfr13I GGNCC 4 cut(s) 629, 1592, 2169, 2209
CseI GACGC 1 cut(s) 562
Csp6I GTAC 4 cut(s) 1024, 1251, 1604, 1826
CviAII CATG 2 cut(s) 1927, 1946
CviQI GTAC 4 cut(s) 1024, 1251, 1604, 1826
DdeI CTNAG 9 cut(s) 123, 273, 423, 569, 951, 1257, 1830, 2183, 2230
DpnI GATC 3 cut(s) 971, 1524, 1530
DpnII GATC 3 cut(s) 969, 1522, 1528
Eam1104I CTCTTC 3 cut(s) 915, 1454, 1815
EarI CTCTTC 3 cut(s) 915, 1454, 1815
Ecl136II GAGCTC 1 cut(s) 1400
Eco105I TACGTA 1 cut(s) 910
Eco24I GRGCYC 3 cut(s) 1263, 1402, 1571
Eco31I GGTCTC 3 cut(s) 99, 249, 399
Eco32I GATATC 2 cut(s) 958, 1837
Eco47I GGWCC 3 cut(s) 629, 1592, 2209
Eco53kI GAGCTC 1 cut(s) 1400
Eco57I CTGAAG 2 cut(s) 1077, 1629
Eco81I CCTNAGG 2 cut(s) 951, 1830
EcoICRI GAGCTC 1 cut(s) 1400
EcoNI CCTNNNNNAGG 1 cut(s) 1094
EcoRII CCWGG 1 cut(s) 2080
EcoRV GATATC 2 cut(s) 958, 1837
EcoT22I ATGCAT 1 cut(s) 1423
EcoT38I GRGCYC 3 cut(s) 1263, 1402, 1571
Esp3I CGTCTC 2 cut(s) 690, 798
FaeI CATG 2 cut(s) 1930, 1949
FalI AAGNNNNNCTT 2 cut(s) 1019, 1051
FaqI GGGAC 2 cut(s) 1232, 2142
FatI CATG 2 cut(s) 1926, 1945
FauI CCCGC 1 cut(s) 766
FbaI TGATCA 1 cut(s) 969
FblI GTMKAC 2 cut(s) 1674, 1781
Fnu4HI GCNGC 8 cut(s) 17, 167, 317, 467, 1041, 1735, 1738, 1803
FokI GGATG 7 cut(s) 103, 253, 403, 718, 1834, 1987, 2006
FriOI GRGCYC 3 cut(s) 1263, 1402, 1571
Fsp4HI GCNGC 8 cut(s) 17, 167, 317, 467, 1041, 1735, 1738, 1803
FspBI CTAG 3 cut(s) 170, 470, 2058
GlaI GCGC 4 cut(s) 812, 1722, 2096, 2107
GluI GCNGC 8 cut(s) 17, 167, 317, 467, 1041, 1735, 1738, 1803
GsaI CCCAGC 4 cut(s) 710, 768, 845, 2101
GsuI CTGGAG 2 cut(s) 516, 1671
HaeII RGCGCY 1 cut(s) 814
HaeIII GGCC 2 cut(s) 1201, 2170
HapII CCGG 3 cut(s) 1595, 1897, 2015
HgaI GACGC 1 cut(s) 562
HhaI GCGC 4 cut(s) 813, 1723, 2097, 2108
Hin1II CATG 2 cut(s) 1930, 1949
Hin6I GCGC 4 cut(s) 811, 1721, 2095, 2106
HinP1I GCGC 4 cut(s) 811, 1721, 2095, 2106
HincII GTYRAC 4 cut(s) 201, 351, 501, 1782
HindII GTYRAC 4 cut(s) 201, 351, 501, 1782
HindIII AAGCTT 1 cut(s) 1756
HpaII CCGG 3 cut(s) 1595, 1897, 2015
HphI GGTGA 5 cut(s) 594, 1394, 1563, 1780, 1921
Hpy166II GTNNAC 7 cut(s) 51, 201, 351, 501, 1675, 1782, 2078
Hpy188I TCNGA 7 cut(s) 1057, 1143, 1426, 1440, 1689, 2233, 2252
Hpy8I GTNNAC 7 cut(s) 51, 201, 351, 501, 1675, 1782, 2078
HpyAV CCTTC 5 cut(s) 862, 1090, 1604, 1650, 1759
HpyCH4III ACNGT 3 cut(s) 985, 1303, 2090
HpyCH4IV ACGT 3 cut(s) 909, 1022, 1534
HpyCH4V TGCA 7 cut(s) 1232, 1421, 1487, 1541, 1625, 1805, 2117
HpyF10VI GCNNNNNNNGC 9 cut(s) 222, 522, 570, 1166, 1406, 1802, 2003, 2103, 2114
HpyF3I CTNAG 9 cut(s) 123, 273, 423, 569, 951, 1257, 1830, 2183, 2230
HpySE526I ACGT 3 cut(s) 909, 1022, 1534
Hsp92II CATG 2 cut(s) 1930, 1949
HspAI GCGC 4 cut(s) 811, 1721, 2095, 2106
Kpn2I TCCGGA 2 cut(s) 1594, 1896
KpnI GGTACC 1 cut(s) 1829
Ksp22I TGATCA 1 cut(s) 969
Kzo9I GATC 3 cut(s) 969, 1522, 1528
LmnI GCTCC 1 cut(s) 1293
Lsp1109I GCAGC 8 cut(s) 3, 153, 303, 453, 1027, 1721, 1724, 1789
LweI GCATC 4 cut(s) 1312, 1408, 1430, 1984
MaeI CTAG 3 cut(s) 170, 470, 2058
MaeII ACGT 3 cut(s) 909, 1022, 1534
MaeIII GTNAC 4 cut(s) 1102, 1460, 1585, 2068
MalI GATC 3 cut(s) 971, 1524, 1530
MbiI CCGCTC 1 cut(s) 1845
MboI GATC 3 cut(s) 969, 1522, 1528
MfeI CAATTG 1 cut(s) 2204
MhlI GDGCHC 4 cut(s) 990, 1263, 1402, 1571
MluCI AATT 6 cut(s) 873, 1128, 1313, 1441, 1679, 2204
MlyI GAGTC 6 cut(s) 1457, 1592, 1787, 1871, 2124, 2148
MmeI TCCRAC 4 cut(s) 683, 1371, 1525, 1945
Mph1103I ATGCAT 1 cut(s) 1423
MroI TCCGGA 2 cut(s) 1594, 1896
MseI TTAA 7 cut(s) 150, 300, 450, 825, 1278, 1413, 1556
MspA1I CMGCKG 1 cut(s) 1734
MspCI CTTAAG 1 cut(s) 1555
MspI CCGG 3 cut(s) 1595, 1897, 2015
MspR9I CCNGG 1 cut(s) 2082
MunI CAATTG 1 cut(s) 2204
Mva1269I GAATGC 3 cut(s) 1476, 1707, 1805
MvaI CCWGG 1 cut(s) 2082
MvnI CGCG 2 cut(s) 2095, 2106
MwoI GCNNNNNNNGC 9 cut(s) 222, 522, 570, 1166, 1406, 1802, 2003, 2103, 2114
NdeII GATC 3 cut(s) 969, 1522, 1528
NlaIII CATG 2 cut(s) 1930, 1949
NlaIV GGNNCC 1 cut(s) 1827
NmuCI GTSAC 2 cut(s) 1102, 1460
NsiI ATGCAT 1 cut(s) 1423
PagI TCATGA 1 cut(s) 1926
PcsI WCGNNNNNNNCGW 1 cut(s) 1531
PctI GAATGC 3 cut(s) 1476, 1707, 1805
PfeI GAWTC 9 cut(s) 61, 211, 361, 511, 926, 946, 1181, 1561, 1771
PflFI GACNNNGTC 1 cut(s) 1223
PkrI GCNGC 8 cut(s) 18, 168, 318, 468, 1042, 1736, 1739, 1804
Ple19I CGATCG 1 cut(s) 1525
PleI GAGTC 6 cut(s) 1457, 1592, 1786, 1870, 2124, 2148
PpsI GAGTC 6 cut(s) 1457, 1592, 1786, 1870, 2124, 2148
Ppu21I YACGTR 1 cut(s) 910
PshBI ATTAAT 1 cut(s) 1278
Psp124BI GAGCTC 1 cut(s) 1402
Psp1406I AACGTT 1 cut(s) 1534
Psp6I CCWGG 1 cut(s) 2080
PspFI CCCAGC 4 cut(s) 706, 764, 841, 2097
PspGI CCWGG 1 cut(s) 2080
PspN4I GGNNCC 1 cut(s) 1827
PspPI GGNCC 4 cut(s) 629, 1592, 2169, 2209
PstI CTGCAG 1 cut(s) 1543
PstNI CAGNNNCTG 1 cut(s) 1737
PsyI GACNNNGTC 1 cut(s) 1223
PvuI CGATCG 1 cut(s) 1525
PvuII CAGCTG 1 cut(s) 1734
RsaI GTAC 4 cut(s) 1025, 1252, 1605, 1827
RsaNI GTAC 4 cut(s) 1024, 1251, 1604, 1826
SacI GAGCTC 1 cut(s) 1402
SalI GTCGAC 1 cut(s) 1780
SaqAI TTAA 7 cut(s) 150, 300, 450, 825, 1278, 1413, 1556
SatI GCNGC 8 cut(s) 17, 167, 317, 467, 1041, 1735, 1738, 1803
Sau3AI GATC 3 cut(s) 969, 1522, 1528
Sau96I GGNCC 4 cut(s) 629, 1592, 2169, 2209
ScaI AGTACT 1 cut(s) 1252
SchI GAGTC 6 cut(s) 1457, 1592, 1787, 1871, 2124, 2148
ScrFI CCNGG 1 cut(s) 2082
SduI GDGCHC 4 cut(s) 990, 1263, 1402, 1571
SfaNI GCATC 4 cut(s) 1312, 1408, 1430, 1984
SfcI CTRYAG 2 cut(s) 1539, 2173
SinI GGWCC 3 cut(s) 629, 1592, 2209
SmlI CTYRAG 2 cut(s) 1555, 2132
SmoI CTYRAG 2 cut(s) 1555, 2132
SnaBI TACGTA 1 cut(s) 910
SpeI ACTAGT 1 cut(s) 2057
Sse9I AATT 6 cut(s) 873, 1128, 1313, 1441, 1679, 2204
SsiI CCGC 4 cut(s) 759, 1843, 2004, 2093
SspI AATATT 4 cut(s) 42, 192, 342, 492
SspMI CTAG 3 cut(s) 170, 470, 2058
SstI GAGCTC 1 cut(s) 1402
StyD4I CCNGG 1 cut(s) 2080
TaaI ACNGT 3 cut(s) 985, 1303, 2090
TaiI ACGT 3 cut(s) 912, 1025, 1537
TaqI TCGA 5 cut(s) 1521, 1525, 1781, 1919, 1939
TasI AATT 6 cut(s) 873, 1128, 1313, 1441, 1679, 2204
TatI WGTACW 1 cut(s) 1250
TfiI GAWTC 9 cut(s) 61, 211, 361, 511, 926, 946, 1181, 1561, 1771
Tru1I TTAA 7 cut(s) 150, 300, 450, 825, 1278, 1413, 1556
Tru9I TTAA 7 cut(s) 150, 300, 450, 825, 1278, 1413, 1556
TscAI CASTG 2 cut(s) 1245, 2196
TseFI GTSAC 2 cut(s) 1102, 1460
TseI GCWGC 8 cut(s) 16, 166, 316, 466, 1040, 1734, 1737, 1802
Tsp45I GTSAC 2 cut(s) 1102, 1460
TspGWI ACGGA 3 cut(s) 672, 780, 1799
TspRI CASTG 2 cut(s) 1245, 2196
Tth111I GACNNNGTC 1 cut(s) 1223
Vha464I CTTAAG 1 cut(s) 1555
VpaK11BI GGWCC 3 cut(s) 629, 1592, 2209
VspI ATTAAT 1 cut(s) 1278
XagI CCTNNNNNAGG 1 cut(s) 1094
XapI RAATTY 3 cut(s) 1128, 1313, 1441
XcmI CCANNNNNNNNNTGG 2 cut(s) 87, 387
XmiI GTMKAC 2 cut(s) 1674, 1781
XspI CTAG 3 cut(s) 170, 470, 2058
ZrmI AGTACT 1 cut(s) 1252
Zsp2I ATGCAT 1 cut(s) 1423
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.