Prupe.1G070300_v2.0.a1

RNA helicase SDE3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0000595
Physical Location & Seq
Forward (+)
4989933 .. 4993223
3291 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G070300.1

Sequence Viewer

Length: 2145 bp
ATGGTCGTTTGGTTTCTTTGTTTTGTTCACAGAAAGAGGAAAATCATGTCATTTTTTCGCGAACTCTTGAAATGGCTTCCCTTCCAACCCGAATCACAGGATCGTGATACTTGCAACAGAAACCGCTTGCCACATTCATCTGAACCCCCATCAACTCCATCTAAACCAGTCCTGTCTCCCTCTAATCCACCTTCATCTTCCCCTACACCTCCTACATTGGGTAGACAACCGCAATCTTCTTCGCTCCATTCATCTTCGTCATCTCCTAAATTCGCAACATCTTCATCTAAGCTACCACCATCACCTTTTGTTAGTCCATCTTCATCATCCCGTCAACCCCCAACACCTTCATCCACATCATCTCCGTTTTCTCCTCAACTATCTACTTACTCCTCTAAGCCACTTCAGACCTCATGGTCTCCGGCTTCATCTGAGCCACATTCATTATCCACAAATCCCCCAACATCTTCTTCAAAACCATCTCCATCTTCTTCAAATCAGCAATCTTCTTCTATCAGTACATCTTCTCCATCTCCTAAATTCCCAACATCTTTATCTAAAACACAACCATCTTCTTCATCTAAGCTGCCTCAATCAACATTCCCTGAGCCCCCATCATCTTCATCTAAGCTGCCTCAATCAACTTTTGTTAATCCATCTTCATCTAAACTGTCTACATCCTCCTCTAAACCACCTCAGGCTTCATCAAAATCACATTCATCATCACCAAATCCTTCAACATCTCCAAAACCGTCTCCATCCTCGGGACTGCCTGTGCCTTCCAATGAGTTCCCTCTAATCTTTAAGCAAGTTCTATCCCCAGCCTCCTCAAATGTAATAAATGAAGGGGGGGAGAAAAATTATGTATGTGTTGATATTGTGCCTATATTCACGATTCCTGAGGATATCAAAGATTTGATCAAGAATGACATTGTGCCTAAAGTTCTTAAACAGCCTCTGTCTCCCACGACATATAAGGATTACTTTGCTGCTCTGTTATATGCTGAAGAGTTCTACCATGAGAAATGGGCTGATTTCAATATGAAGAATGTGACACTGAAGTTGCATGAAGCAGCAGTTTATAAAAATAAAGACAAGGAAGAGAAAACCTTCGCAGAATTTGAGATTGATTCTGTTCCTGAAAATCGGCCATTCCTTTTGTCGAGGGACTTAGTCCATGTACGGCCATCAGGTACAAATGCTGAGCAGTTTCAGGGCATTATACATCGCATTATTAGGAGCAATCTTGTATTAGTGGAATTTGAAGATGAATTTTATGATTATCATTATTCGACCCAAAAATATGATGTCAGCTTCTCATTCAATAGAGTATGTTTGAAAAGAGCTCACCATGCAGTTCAAACAGCATCGGATACTTTGTTTAAGAACTTCCTCTTTCCCGATTGTGTTTCCCGTACGAGCATTCCCACTGCACCAGCGCTGCCGCCTGGCCGTCACAAACTTGACGCCAAACAATTATCTGCTGTTCGTCAAATTTTAAGCATTCAGGGCTCACCACCTTACTTGGTGGCTGGCCAGCTTGGTGTTGAAAGGAACGTTTTTAGGCCGTCGAGAACTGGAGCGGTCGTTTGTGAAGCAGTGCACCAGTTATGTCAAACCTCACTCAAAAATAGGATTCTGATATGTGCTCCTAGTAACCGCTGTTGTGATGGGATTATGAGAAGCTTGTTGGAAGTGATTCCAGAGTCAGATATGTTTCGAGCCAACGCTGCATTCCGTGAGAAAGATGAGGTGCCTGATGACATCCTCCTATCATGCCTTTACGAAGACCCTTATTTCTCTTGTCCTCCAACTGAGAAGCTCAAAAAATTCAGGGTGATTTTCTCAACTCTCATGAGTAGCTTTCGGCTACATGATAAAGGCCTAACTTCTGGACATTTTAGTCATATTTTTCTGGTAGATGCTTCATCTGCCATTGAGCCGGAAACAGCAGTGGCCTTAACTAATTTTGCAGAAAAAAGTACTACTGTTATAGTCACTGGTCAACCAGGAGATAATTCACGTTGGGTTCGCGCAGACATGGCAAGACAACAGGGACTGAAGATTTCATACTTCGAGAGACTTTTCAAGTCAAGGCCATATAGAAGCCTCAATCCAATGCTAATCACACAATTGGACCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

715

Amino Acids

79.12

Weight (kDa)

9.17

Isoelectric Point (pI)

69.49

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000595)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g37100 FvH4_4g37100 FvH4_4g37100
malus_domestica MD13G1238300.v1.1 MD16G1243100.v1.1 MD16G1243200.v1.1
prunus_persica Prupe.1G070100_v2.0.a1 Prupe.1G070200_v2.0.a1 Prupe.1G070300_v2.0.a1 Prupe.1G070300_v2.0.a1
pyrus_communis pycom13g21070 pycom16g20390 pycom16g20400
rosa_chinensis RchiOBHm_Chr1g0318071 RchiOBHm_Chr1g0318111 RchiOBHm_Chr4g0446861 RchiOBHm_Chr4g0446881 RchiOBHm_Chr4g0446891
rosa_laevigata RLG00000005623 RLG00000005624 RLG00000009337 RLG00000030560 RLG00000030566
rosa_multiflora Rmu_co8177450.1_g000001 Rmu_sc0001753.1_g000012 Rmu_sc0001753.1_g000013 Rmu_sc0001753.1_g000014 Rmu_sc0001753.1_g000015 Rmu_sc0011424.1_g000009 Rmu_sc0011424.1_g000020 Rmu_sc0034555.1_g000001 Rmu_ssc0000008.1_g000002
rosa_roxburghii Rroxscaffold_4G00329530 Rroxscaffold_4G00329590 Rroxscaffold_4G00329800 Rroxscaffold_4G00329810 Rroxscaffold_4G00329860 Rroxscaffold_4G00329910 Rroxscaffold_4G00330030 Rroxscaffold_5G00343610 Rroxscaffold_5G00348280 Rroxscaffold_5G00387220 Rroxscaffold_5G00387230
rosa_rugosa Rorug01G0019100 Rorug01G0019100 Rorug01G0019500 Rorug01G0019600 Rorug04G0010100 Rorug04G0375200 Rorug04G0375300 Rorug04G0375400 Rorug04G0375500 Rorug04G0375600 Rorug04G0375700
rosa_samantha Rh1AG030700 Rh1AG030900 Rh1BG026900 Rh1CG029500 Rh1CG029700 Rh1DG040500 Rh4AG088100 Rh4AG436700 Rh4AG436800 Rh4AG436900 Rh4BG084700 Rh4BG445800 Rh4BG445900 Rh4BG446100 Rh4CG096300 Rh4CG463100 Rh4CG463200 Rh4CG463400 Rh4DG444500 Rh4DG444600 Rh4DG444700
rosa_wichuraiana Rw1G002420 Rw1G002440 Rw1G002480 Rw4G037360 Rw4G037370 Rw4G037380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1083
AasI GACNNNNNNGTC 2 cut(s) 415, 1902
AccB1I GGYRCC 1 cut(s) 1753
AccBSI CCGCTC 1 cut(s) 1583
AccI GTMKAC 2 cut(s) 223, 674
AccII CGCG 2 cut(s) 60, 2034
AciI CCGC 5 cut(s) 124, 230, 1445, 1583, 1660
AclI AACGTT 1 cut(s) 1557
AclWI GGATC 1 cut(s) 108
AcoI YGGCCR 4 cut(s) 1148, 1184, 1450, 1534
AcsI RAATTY 7 cut(s) 269, 539, 1118, 1259, 1271, 1494, 1829
AcuI CTGAAG 4 cut(s) 389, 1026, 1079, 2081
AcyI GRCGYC 1 cut(s) 1467
AfaI GTAC 5 cut(s) 520, 1182, 1195, 1417, 1984
AfeI AGCGCT 1 cut(s) 1440
AfiI CCNNNNNNNGG 2 cut(s) 218, 764
AjnI CCWGG 2 cut(s) 1447, 2008
AjuI GAANNNNNNNTTGG 4 cut(s) 454, 486, 1718, 1750
AluBI AGCT 9 cut(s) 292, 586, 631, 1314, 1346, 1540, 1686, 1822, 1863
AluI AGCT 9 cut(s) 292, 586, 631, 1314, 1346, 1540, 1686, 1822, 1863
Alw21I GWGCWC 3 cut(s) 1348, 1605, 1651
Alw26I GTCTC 5 cut(s) 180, 423, 759, 966, 2074
Alw44I GTGCAC 1 cut(s) 1601
AlwI GGATC 1 cut(s) 108
AlwNI CAGNNNCTG 2 cut(s) 958, 2059
Ama87I CYCGRG 1 cut(s) 763
Aor51HI AGCGCT 1 cut(s) 1440
AoxI GGCC 8 cut(s) 1148, 1184, 1450, 1534, 1565, 1882, 1956, 2096
ApaLI GTGCAC 1 cut(s) 1601
ApeKI GCWGC 6 cut(s) 586, 631, 989, 1073, 1441, 1730
ApoI RAATTY 7 cut(s) 269, 539, 1118, 1259, 1271, 1494, 1829
Asp700I GAANNNNTTC 2 cut(s) 1109, 1698
AspLEI GCGC 2 cut(s) 1441, 2036
AspS9I GGNCC 1 cut(s) 2137
AsuHPI GGTGA 5 cut(s) 294, 717, 1340, 1506, 1849
AvaI CYCGRG 1 cut(s) 763
AvaII GGWCC 1 cut(s) 2137
AxyI CCTNAGG 2 cut(s) 696, 900
BaeGI GKGCMC 1 cut(s) 1605
BalI TGGCCA 1 cut(s) 1536
BanI GGYRCC 1 cut(s) 1753
BanII GRGCYC 3 cut(s) 612, 1348, 1514
BarI GAAGNNNNNNTAC 2 cut(s) 2054, 2086
BbsI GAAGAC 1 cut(s) 1794
Bbv12I GWGCWC 3 cut(s) 1348, 1605, 1651
BbvI GCAGC 6 cut(s) 573, 618, 976, 1085, 1428, 1717
BceAI ACGGC 3 cut(s) 1199, 1437, 1552
BciT130I CCWGG 2 cut(s) 1449, 2010
BciVI GTATCC 1 cut(s) 1366
BclI TGATCA 1 cut(s) 918
BcoDI GTCTC 5 cut(s) 180, 423, 759, 966, 2074
BfaI CTAG 1 cut(s) 1653
BfoI RGCGCY 1 cut(s) 1442
BfuI GTATCC 1 cut(s) 1366
BisI GCNGC 7 cut(s) 587, 632, 990, 1074, 1442, 1445, 1731
BlpI GCTNAGC 1 cut(s) 1203
BlsI GCNGC 7 cut(s) 588, 633, 991, 1075, 1443, 1446, 1732
BmcAI AGTACT 1 cut(s) 1984
Bme1390I CCNGG 2 cut(s) 1449, 2010
Bme18I GGWCC 1 cut(s) 2137
BmeT110I CYCGRG 1 cut(s) 763
BmgT120I GGNCC 1 cut(s) 2137
BmiI GGNNCC 1 cut(s) 1755
BmrFI CCNGG 2 cut(s) 1449, 2010
BmsI GCATC 2 cut(s) 1376, 1912
BpiI GAAGAC 1 cut(s) 1794
BpmI CTGGAG 1 cut(s) 1599
Bpu10I CCTNAGC 1 cut(s) 606
Bpu1102I GCTNAGC 1 cut(s) 1203
BsaHI GRCGYC 1 cut(s) 1467
BsaI GGTCTC 1 cut(s) 423
BsaJI CCNNGG 1 cut(s) 762
BsaXI ACNNNNNCTCC 6 cut(s) 346, 376, 511, 541, 1572, 1602
Bsc4I CCNNNNNNNGG 2 cut(s) 218, 764
Bse1I ACTGG 5 cut(s) 167, 1582, 1606, 2005, 2140
Bse21I CCTNAGG 2 cut(s) 696, 900
BseBI CCWGG 2 cut(s) 1449, 2010
BseDI CCNNGG 1 cut(s) 762
BseGI GGATG 5 cut(s) 326, 350, 677, 758, 1764
BseLI CCNNNNNNNGG 2 cut(s) 218, 764
BseMII CTCAG 6 cut(s) 423, 597, 710, 891, 1194, 1806
BseNI ACTGG 5 cut(s) 167, 1582, 1606, 2005, 2140
BseRI GAGGAG 4 cut(s) 363, 382, 673, 817
BseSI GKGCMC 1 cut(s) 1605
BseXI GCAGC 6 cut(s) 573, 618, 976, 1085, 1428, 1717
BseYI CCCAGC 1 cut(s) 820
BsgI GTGCAG 1 cut(s) 1416
Bsh1236I CGCG 2 cut(s) 60, 2034
Bsh1285I CGRYCG 1 cut(s) 1587
BshFI GGCC 8 cut(s) 1150, 1186, 1452, 1536, 1567, 1884, 1958, 2098
BshNI GGYRCC 1 cut(s) 1753
BsiEI CGRYCG 1 cut(s) 1587
BsiHKAI GWGCWC 3 cut(s) 1348, 1605, 1651
BsiHKCI CYCGRG 1 cut(s) 763
BsiSI CCGG 2 cut(s) 422, 1943
BsiWI CGTACG 1 cut(s) 1415
BslFI GGGAC 3 cut(s) 780, 1181, 2070
BslI CCNNNNNNNGG 2 cut(s) 218, 764
BsmAI GTCTC 5 cut(s) 180, 423, 759, 966, 2074
BsmBI CGTCTC 1 cut(s) 759
BsmFI GGGAC 3 cut(s) 780, 1181, 2070
BsmI GAATGC 3 cut(s) 1422, 1503, 1733
BsnI GGCC 8 cut(s) 1150, 1186, 1452, 1536, 1567, 1884, 1958, 2098
Bso31I GGTCTC 1 cut(s) 423
BsoBI CYCGRG 1 cut(s) 763
Bsp1286I GDGCHC 5 cut(s) 612, 1348, 1514, 1605, 1651
Bsp143I GATC 2 cut(s) 100, 918
Bsp1720I GCTNAGC 1 cut(s) 1203
Bsp68I TCGCGA 1 cut(s) 60
BspACI CCGC 5 cut(s) 124, 230, 1445, 1583, 1660
BspANI GGCC 8 cut(s) 1150, 1186, 1452, 1536, 1567, 1884, 1958, 2098
BspCNI CTCAG 6 cut(s) 424, 598, 709, 892, 1195, 1807
BspFNI CGCG 2 cut(s) 60, 2034
BspHI TCATGA 1 cut(s) 1854
BspLI GGNNCC 1 cut(s) 1755
BspPI GGATC 1 cut(s) 108
BspT107I GGYRCC 1 cut(s) 1753
BspTNI GGTCTC 1 cut(s) 423
BsrBI CCGCTC 1 cut(s) 1583
BsrI ACTGG 5 cut(s) 167, 1582, 1606, 2005, 2140
BssECI CCNNGG 1 cut(s) 762
BssMI GATC 2 cut(s) 100, 918
BssNI GRCGYC 1 cut(s) 1467
Bst2UI CCWGG 2 cut(s) 1449, 2010
Bst4CI ACNGT 3 cut(s) 672, 753, 1990
Bst6I CTCTTC 2 cut(s) 1002, 1095
BstACI GRCGYC 1 cut(s) 1467
BstC8I GCNNGC 3 cut(s) 128, 1534, 1538
BstF5I GGATG 5 cut(s) 326, 350, 677, 758, 1764
BstFNI CGCG 2 cut(s) 60, 2034
BstH2I RGCGCY 1 cut(s) 1442
BstHHI GCGC 2 cut(s) 1441, 2036
BstKTI GATC 2 cut(s) 103, 921
BstMAI GTCTC 5 cut(s) 180, 423, 759, 966, 2074
BstMBI GATC 2 cut(s) 100, 918
BstMCI CGRYCG 1 cut(s) 1587
BstMWI GCNNNNNNNGC 5 cut(s) 1352, 1509, 1730, 1931, 2042
BstNI CCWGG 2 cut(s) 1449, 2010
BstSCI CCNGG 2 cut(s) 1447, 2008
BstSLI GKGCMC 1 cut(s) 1605
BstUI CGCG 2 cut(s) 60, 2034
BstV1I GCAGC 6 cut(s) 573, 618, 976, 1085, 1428, 1717
BstV2I GAAGAC 1 cut(s) 1794
Bsu36I CCTNAGG 2 cut(s) 696, 900
BsuI GTATCC 1 cut(s) 1366
BsuRI GGCC 8 cut(s) 1150, 1186, 1452, 1536, 1567, 1884, 1958, 2098
BtgZI GCGATG 1 cut(s) 1211
BtsCI GGATG 5 cut(s) 326, 350, 677, 758, 1764
BtsI GCAGTG 3 cut(s) 1428, 1605, 1959
BtsIMutI CAGTG 5 cut(s) 1055, 1428, 1605, 1959, 1998
BtuMI TCGCGA 1 cut(s) 60
Cac8I GCNNGC 3 cut(s) 128, 1534, 1538
CaiI CAGNNNCTG 2 cut(s) 958, 2059
CciI TCATGA 1 cut(s) 1854
CfoI GCGC 2 cut(s) 1441, 2036
Cfr13I GGNCC 1 cut(s) 2137
CseI GACGC 1 cut(s) 1475
Csp6I GTAC 5 cut(s) 519, 1181, 1194, 1416, 1983
CspCI CAANNNNNGTGG 2 cut(s) 1506, 1541
CviQI GTAC 5 cut(s) 519, 1181, 1194, 1416, 1983
DpnI GATC 2 cut(s) 102, 920
DpnII GATC 2 cut(s) 100, 918
DrdI GACNNNNNNGTC 2 cut(s) 415, 1902
DseDI GACNNNNNNGTC 2 cut(s) 415, 1902
EaeI YGGCCR 4 cut(s) 1148, 1184, 1450, 1534
Eam1104I CTCTTC 2 cut(s) 1002, 1095
EarI CTCTTC 2 cut(s) 1002, 1095
Ecl136II GAGCTC 1 cut(s) 1346
Eco147I AGGCCT 1 cut(s) 1884
Eco24I GRGCYC 3 cut(s) 612, 1348, 1514
Eco31I GGTCTC 1 cut(s) 423
Eco32I GATATC 1 cut(s) 907
Eco47I GGWCC 1 cut(s) 2137
Eco47III AGCGCT 1 cut(s) 1440
Eco53kI GAGCTC 1 cut(s) 1346
Eco57I CTGAAG 4 cut(s) 389, 1026, 1079, 2081
Eco81I CCTNAGG 2 cut(s) 696, 900
Eco88I CYCGRG 1 cut(s) 763
EcoICRI GAGCTC 1 cut(s) 1346
EcoRII CCWGG 2 cut(s) 1447, 2008
EcoRV GATATC 1 cut(s) 907
EcoT38I GRGCYC 3 cut(s) 612, 1348, 1514
Esp3I CGTCTC 1 cut(s) 759
FalI AAGNNNNNCTT 2 cut(s) 968, 1000
FaqI GGGAC 3 cut(s) 780, 1181, 2070
FbaI TGATCA 1 cut(s) 918
FblI GTMKAC 2 cut(s) 223, 674
Fnu4HI GCNGC 7 cut(s) 587, 632, 990, 1074, 1442, 1445, 1731
FokI GGATG 5 cut(s) 313, 337, 664, 745, 1751
FriOI GRGCYC 3 cut(s) 612, 1348, 1514
Fsp4HI GCNGC 7 cut(s) 587, 632, 990, 1074, 1442, 1445, 1731
FspBI CTAG 1 cut(s) 1653
GlaI GCGC 2 cut(s) 1440, 2035
GluI GCNGC 7 cut(s) 587, 632, 990, 1074, 1442, 1445, 1731
GsaI CCCAGC 1 cut(s) 824
GsuI CTGGAG 1 cut(s) 1599
HaeII RGCGCY 1 cut(s) 1442
HaeIII GGCC 8 cut(s) 1150, 1186, 1452, 1536, 1567, 1884, 1958, 2098
HapII CCGG 2 cut(s) 422, 1943
HgaI GACGC 1 cut(s) 1475
HhaI GCGC 2 cut(s) 1441, 2036
Hin1I GRCGYC 1 cut(s) 1467
Hin6I GCGC 2 cut(s) 1439, 2034
HinP1I GCGC 2 cut(s) 1439, 2034
HincII GTYRAC 2 cut(s) 335, 2006
HindII GTYRAC 2 cut(s) 335, 2006
HindIII AAGCTT 1 cut(s) 1684
HinfI GANTC 6 cut(s) 92, 895, 1130, 1636, 1699, 1706
HpaII CCGG 2 cut(s) 422, 1943
HphI GGTGA 5 cut(s) 294, 717, 1340, 1506, 1849
Hpy166II GTNNAC 6 cut(s) 28, 224, 335, 675, 1603, 2006
Hpy188I TCNGA 6 cut(s) 142, 408, 433, 1372, 1641, 1711
Hpy8I GTNNAC 6 cut(s) 28, 224, 335, 675, 1603, 2006
Hpy99I CGWCG 1 cut(s) 1573
HpyAV CCTTC 7 cut(s) 91, 201, 357, 744, 789, 839, 1120
HpyCH4III ACNGT 3 cut(s) 672, 753, 1990
HpyCH4IV ACGT 2 cut(s) 1557, 2023
HpyCH4V TGCA 7 cut(s) 114, 1066, 1355, 1433, 1603, 1733, 1973
HpyF10VI GCNNNNNNNGC 5 cut(s) 1352, 1509, 1730, 1931, 2042
HpySE526I ACGT 2 cut(s) 1557, 2023
Hsp92I GRCGYC 1 cut(s) 1467
HspAI GCGC 2 cut(s) 1439, 2034
Ksp22I TGATCA 1 cut(s) 918
Kzo9I GATC 2 cut(s) 100, 918
LmnI GCTCC 4 cut(s) 249, 1239, 1580, 1654
Lsp1109I GCAGC 6 cut(s) 573, 618, 976, 1085, 1428, 1717
LweI GCATC 2 cut(s) 1376, 1912
MaeI CTAG 1 cut(s) 1653
MaeII ACGT 2 cut(s) 1557, 2023
MaeIII GTNAC 4 cut(s) 1051, 1454, 1655, 1996
MalI GATC 2 cut(s) 102, 920
MbiI CCGCTC 1 cut(s) 1583
MboI GATC 2 cut(s) 100, 918
MfeI CAATTG 1 cut(s) 2132
MhlI GDGCHC 5 cut(s) 612, 1348, 1514, 1605, 1651
MlsI TGGCCA 1 cut(s) 1536
MluNI TGGCCA 1 cut(s) 1536
MlyI GAGTC 1 cut(s) 1715
MmeI TCCRAC 3 cut(s) 109, 1671, 1835
Mox20I TGGCCA 1 cut(s) 1536
MroXI GAANNNNTTC 2 cut(s) 1109, 1698
MscI TGGCCA 1 cut(s) 1536
MseI TTAA 6 cut(s) 651, 804, 948, 1383, 1499, 1961
Msp20I TGGCCA 1 cut(s) 1536
MspA1I CMGCKG 1 cut(s) 1662
MspI CCGG 2 cut(s) 422, 1943
MspR9I CCNGG 2 cut(s) 1449, 2010
MunI CAATTG 1 cut(s) 2132
Mva1269I GAATGC 3 cut(s) 1422, 1503, 1733
MvaI CCWGG 2 cut(s) 1449, 2010
MvnI CGCG 2 cut(s) 60, 2034
MwoI GCNNNNNNNGC 5 cut(s) 1352, 1509, 1730, 1931, 2042
NdeII GATC 2 cut(s) 100, 918
NlaIV GGNNCC 1 cut(s) 1755
NmuCI GTSAC 3 cut(s) 1051, 1454, 1996
NruI TCGCGA 1 cut(s) 60
PagI TCATGA 1 cut(s) 1854
PceI AGGCCT 1 cut(s) 1884
PctI GAATGC 3 cut(s) 1422, 1503, 1733
PdmI GAANNNNTTC 2 cut(s) 1109, 1698
PfeI GAWTC 5 cut(s) 92, 895, 1130, 1636, 1699
Pfl23II CGTACG 1 cut(s) 1415
PflFI GACNNNGTC 1 cut(s) 1172
PkrI GCNGC 7 cut(s) 588, 633, 991, 1075, 1443, 1446, 1732
PleI GAGTC 1 cut(s) 1714
PpsI GAGTC 1 cut(s) 1714
PsiI TTATAA 1 cut(s) 1083
Psp124BI GAGCTC 1 cut(s) 1348
Psp1406I AACGTT 1 cut(s) 1557
Psp6I CCWGG 2 cut(s) 1447, 2008
PspFI CCCAGC 1 cut(s) 820
PspGI CCWGG 2 cut(s) 1447, 2008
PspLI CGTACG 1 cut(s) 1415
PspN4I GGNNCC 1 cut(s) 1755
PspPI GGNCC 1 cut(s) 2137
PstNI CAGNNNCTG 2 cut(s) 958, 2059
PsyI GACNNNGTC 1 cut(s) 1172
RruI TCGCGA 1 cut(s) 60
RsaI GTAC 5 cut(s) 520, 1182, 1195, 1417, 1984
RsaNI GTAC 5 cut(s) 519, 1181, 1194, 1416, 1983
SacI GAGCTC 1 cut(s) 1348
SaqAI TTAA 6 cut(s) 651, 804, 948, 1383, 1499, 1961
SatI GCNGC 7 cut(s) 587, 632, 990, 1074, 1442, 1445, 1731
Sau3AI GATC 2 cut(s) 100, 918
Sau96I GGNCC 1 cut(s) 2137
ScaI AGTACT 1 cut(s) 1984
SchI GAGTC 1 cut(s) 1715
ScrFI CCNGG 2 cut(s) 1449, 2010
SduI GDGCHC 5 cut(s) 612, 1348, 1514, 1605, 1651
SfaNI GCATC 2 cut(s) 1376, 1912
SinI GGWCC 1 cut(s) 2137
SseBI AGGCCT 1 cut(s) 1884
SsiI CCGC 5 cut(s) 124, 230, 1445, 1583, 1660
SspMI CTAG 1 cut(s) 1653
SstI GAGCTC 1 cut(s) 1348
StuI AGGCCT 1 cut(s) 1884
StyD4I CCNGG 2 cut(s) 1447, 2008
TaaI ACNGT 3 cut(s) 672, 753, 1990
TaiI ACGT 2 cut(s) 1560, 2026
TaqI TCGA 5 cut(s) 1163, 1292, 1571, 1720, 2076
TatI WGTACW 2 cut(s) 518, 1982
TauI GCSGC 1 cut(s) 1447
TfiI GAWTC 5 cut(s) 92, 895, 1130, 1636, 1699
Tru1I TTAA 6 cut(s) 651, 804, 948, 1383, 1499, 1961
Tru9I TTAA 6 cut(s) 651, 804, 948, 1383, 1499, 1961
TscAI CASTG 5 cut(s) 1062, 1435, 1605, 1959, 2005
TseFI GTSAC 3 cut(s) 1051, 1454, 1996
TseI GCWGC 6 cut(s) 586, 631, 989, 1073, 1441, 1730
Tsp45I GTSAC 3 cut(s) 1051, 1454, 1996
TspGWI ACGGA 2 cut(s) 354, 1727
TspRI CASTG 5 cut(s) 1062, 1435, 1605, 1959, 2005
Tth111I GACNNNGTC 1 cut(s) 1172
VneI GTGCAC 1 cut(s) 1601
VpaK11BI GGWCC 1 cut(s) 2137
XapI RAATTY 7 cut(s) 269, 539, 1118, 1259, 1271, 1494, 1829
XmiI GTMKAC 2 cut(s) 223, 674
XmnI GAANNNNTTC 2 cut(s) 1109, 1698
XspI CTAG 1 cut(s) 1653
ZrmI AGTACT 1 cut(s) 1984
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.