Rorug01G0019500

RNA helicase SDE3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0000595
Physical Location & Seq
Reverse (-)
3228908 .. 3234049
5142 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0019500.1

Sequence Viewer

Length: 3648 bp
ATGAAGAGGCTGAGATCCAGCGAGGATCTCGATTCTTACGGTAAGGATCCGAGCCCGAACCCGAACCCGAACCCGAGCCGGACCTCCTCCTCCTCTTCCCACCGAAGCTTCTACTACAACAAACCAGACACCGCCCGCAAGGGCTTGCTCTCTTCCTCCTCCTCCGCGTCTTCTCTGGCGGCGGCGGCGGCGAGGTCGTACGACCGGGACCGGGGAGACGACGATCGCGAGTTGGCGGCCGGCGGCGGAGGGTCGAGGATGGTGAGGAAGAGGCCGGCGGACCTCGACGGAGGGTTTGATCGGAGGAAGGGGGTGGATCGGTATAACAGAGACGGCGGCGGCGGAGGGTACGATCGTAATTTGATGCACCGGTCGGAGAGCTTCTGCGGCGGCGGGTCGAGGAGCAGGGCGTCGGAGTTTCCGAAAGGGTTCCGATCGGAGAGGGACCGGTCGCGGCGGGAAGGGAGCAGCGCGCTGTCGTGGCGGCGGTTCGGGAAGGAGTTTGAGGAAGGAGGGAGGAGTAGTGGTAGTAGTAGGTTGGAGGAGAGAGGGAAAGGAGGTGGAGGGTTTAGCGTCCGGTCGCCGTCGTGGTCGAATTCGAGGGATTCCGGCAGCGAGCAATCGAGGGTTAGGTCTCCGCCGAGGAGATTTAAAGACGGGAAATCGTCGAAATCGAAATCAAAGTCTAAGTCGCCGACTTGGTCCAAAGATTCTGTTGGGAGTGAGCAATCCAAGAGTGTTGAGGTGAAGAAGAGTGAGCCTGAGACCGAGCCGGAGCCGGAGCCCGAACCTGAGAGGGAGCCGGAGCCGGAGCCCGAACCTGAACCTGAACCTGAACCTGAGCCGGAGCCGGAGCCGGAGCCGGAACCTATGCCAGAGCCTGAGGCCGAGGCTGTGGTGGAGGAGGTTCAGGGTGAGAGTGGAAGTAGGACTAGTAGTGAAATGGAGGAAGGAGAGCTTGAGCCCGAAGCCGAACCGGAAGATGGGGATGGAGGTGAAGAGCTAGAGGTGGTGCCTGAAGGTGGAGCTGAAATGGAAGAAGGCCGAGTTCAGATTGGGAGCGAAACGGAGACTACGGTAATGGAGGAGAGAGATGGGTGCTTAGATAAAGAAGAGGTGAAGGAGGGAGTGTGTGAGGGGAAAGAGGAGGAAAAGAAAGAAGATGAGTTGCCTTCGGTTGATGAGACCAGAAATGTAGGGAGGCAGGAAGAAGGGAATGAGTGCAAGGAGGAAGTGAGCAAGGAAGTGGTTTCGGAGAGGTCGTTGGAAGTGGACGAGGAGCCAAGGCAAGACAAGGGGATAGACCTTGAAGTGAAGGCTGAGGATGACGAAATGACAGAGTCTGATAGGGAAGTGACAGAGGACACTGGAGTTCATACACTGAATCTGAGTGCGGATTTGACTCGGAATTTCAAGGACAAAGGGAAAAGCGTGGCCCATGTGGAGGATTCAGCAGAAAATAGTAGATGGGTTGAGAGAGAATCCAGAGAACTACTAACTTGCATGGACAATGATATGGAAGGACCGAGCACTAGGGGTTTCGAGTTGTTTACATCCTCTCCTGTTAGGAGGCAGGAGAAAGCTGATTCTGGTGTTAATATGAAAGATGAGAAGTTGGTACTTGAGCCGCTTGACCTTTCACTAAGCTTGCCTAATGTTTTGTTACCTATTGGTGCCACTCCTGGTTCTCCTGATCAAGCAATGAGTGTTCAGTCTTTAAGTAACACTTTCTGTACCAACTCGGATGGATTTACTCAATCAGTATCTTTTTCAGGTTCTCAGTCATTTTATCACAATCCTAGCTGTTCTTTAACGACCCAGAATTCGATGGACTTTGAGCAATCGGTTAAAAGTCGTCCCCTTTTCCAGGGAATTGATTGGCAGGCGCTAGCTCAGAATGAGGCTAAGAATAAGGAGGTTCCATTTTATCAAAAAACTTTGATGACTGGCAATGGTTCTCATCCACAGTCCGGCGTTACAAATGGTCAATCTGTGCAAGGGCAACAAGTTAAACATTCGGAAGGAAGCTCTAAAGTGGCAAATGGATTTGAAAGGCAGCTAAGCTTCCACAAGCAGTTGTCAGGAGGGCACCCAAAGCACCATGAGGATGTTAGGTCACCTTCGCATAGTGTTGGATCTCAAGAAATGGGTTCAAATTATAGTTTTGACAGGAAACGACTAATGAGAGAGAGAAGTAGTGGTAGTTTATATAGGACTAGCAGCCAGAAGGAAAAAGAGCAGTTGCTCCTCAGTGGGGCTGATTTTGTTGAGACAATCATTGCCAGGATAGTATCTGATCCAGTCCATGTAATGGCCAAGAAGTTTCATGAAATGACAGGACAATCTGCAGCATGTGTGAAGGAGAGCATTCGCGAGATTATGTTAAATGTGGATAAGCGCATGCAACTATTTGCATTTCAGAAGGCACTGCAGAACAGGTCTGACATAACCTTGGAGATGCTACTAAAAGCCCATCGGGCACAATTGGAAATATTGGTTGCTCTGAAGACTGGTCTACCAGATTTTCTTCAGCAAGAGAGCAGTGTGTCATCTTCTGATTTGGCTGAGATTTTTCTGTACTTGAGATGCAGAAATCCTTCTTGTCGGAGTCCTGTGCCTGCGGATGAATGTGATTGCAAGGTCTGTTCGCAAAAGAATGGATTTTGCAGCGCTTGTATGTGTCTTGTGTGCTCAAAGTTTGATATGGCCTCAAATACATGTAGTTGGATTGGGTGTGATGTTTGTCTTCATTGGTGCCATGCTGATTGTGCATTGCGGGAATCTTATATTAGAAATGGACGCAGTGCAACTGGATCTCAAGGGACGACTGAGATGCAATTTCATTGTGTTGCTTGTGATCATCCTTCTGAGATGTTTGGCTTTGTGAAGGAGGTTTTTCAGCATTTTGCCAAGGATTGGACGATTGAAAATCTTGCCAGGGAACTTGAATATGTTAAGAGAATTTTCCTTGTCAGCAAGGACATGAGAGGGAGACGGCTGTATGATATTGCTGATCAATCGCTGGTTAGATTGGCAAATAAGTCTGGCCTTCCGGAGGTTTACAGTTACATAATGGCTTTTCTTGAGGATACTGACAGTTCCAAGTTGGGCAAAACTCCCATTTTACCTGGGAAGGATCTAGGTAAAGGAAGCAGTGGTGTTGCTGGGCCAAGCCAGGAACCAGCATGGCTAAAGTCTGTATATACAGCAAAGGCCCCTCAGTTGGAATCTGCTCCTAGCGTTCTTCCTAGCTACAACTATGAGCAGCATGATAAACGCATTGTGGAGTCAGGGTTGCACATGAGTTCTCAAAAAGAACCTGTTTTCGATGAGTTGGAGAGTATTGTGAGGATCAAGCAGGCCGAGGCTAAAATGTTCCAAACACGTGCGGACGAAGCAAGAAAAGAAGCTGAGGGGCTGAAACGCATAGCACTGGCAAAGAATGAGAAAATTGAAGAAGAATATAGGAATAGAATAACAAAACTCCGTTTTGTTGAAGCTGAGGAAATGCGGAAACAGAAACTTGAGGAACTGCAGTCTCTAGATCGAGCTCATCGAGAATATTCCAATATGAAGATGAGGATGGAAGCGGATATTAAAGATCTTTTATTAAAAATGGAAGCAACAAAGCGAAACCTTTCCTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1215

Amino Acids

135.58

Weight (kDa)

5.22

Isoelectric Point (pI)

66.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Trypan_PARP PF05887 226 - 298 5e-07 Procyclic acidic repetitive protein (PARP)
PHD_Oberon PF07227 863 - 986 5.6e-39 PHD - plant homeodomain finger protein
Oberon_cc PF16312 1078 - 1203 2.6e-43 Coiled-coil region of Oberon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000595)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g37100 FvH4_4g37100 FvH4_4g37100
malus_domestica MD13G1238300.v1.1 MD16G1243100.v1.1 MD16G1243200.v1.1
prunus_persica Prupe.1G070100_v2.0.a1 Prupe.1G070200_v2.0.a1 Prupe.1G070300_v2.0.a1 Prupe.1G070300_v2.0.a1
pyrus_communis pycom13g21070 pycom16g20390 pycom16g20400
rosa_chinensis RchiOBHm_Chr1g0318071 RchiOBHm_Chr1g0318111 RchiOBHm_Chr4g0446861 RchiOBHm_Chr4g0446881 RchiOBHm_Chr4g0446891
rosa_laevigata RLG00000005623 RLG00000005624 RLG00000009337 RLG00000030560 RLG00000030566
rosa_multiflora Rmu_co8177450.1_g000001 Rmu_sc0001753.1_g000012 Rmu_sc0001753.1_g000013 Rmu_sc0001753.1_g000014 Rmu_sc0001753.1_g000015 Rmu_sc0011424.1_g000009 Rmu_sc0011424.1_g000020 Rmu_sc0034555.1_g000001 Rmu_ssc0000008.1_g000002
rosa_roxburghii Rroxscaffold_4G00329530 Rroxscaffold_4G00329590 Rroxscaffold_4G00329800 Rroxscaffold_4G00329810 Rroxscaffold_4G00329860 Rroxscaffold_4G00329910 Rroxscaffold_4G00330030 Rroxscaffold_5G00343610 Rroxscaffold_5G00348280 Rroxscaffold_5G00387220 Rroxscaffold_5G00387230
rosa_rugosa Rorug01G0019100 Rorug01G0019100 Rorug01G0019500 Rorug01G0019600 Rorug04G0010100 Rorug04G0375200 Rorug04G0375300 Rorug04G0375400 Rorug04G0375500 Rorug04G0375600 Rorug04G0375700
rosa_samantha Rh1AG030700 Rh1AG030900 Rh1BG026900 Rh1CG029500 Rh1CG029700 Rh1DG040500 Rh4AG088100 Rh4AG436700 Rh4AG436800 Rh4AG436900 Rh4BG084700 Rh4BG445800 Rh4BG445900 Rh4BG446100 Rh4CG096300 Rh4CG463100 Rh4CG463200 Rh4CG463400 Rh4DG444500 Rh4DG444600 Rh4DG444700
rosa_wichuraiana Rw1G002420 Rw1G002440 Rw1G002480 Rw4G037360 Rw4G037370 Rw4G037380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 4 cut(s) 1012, 1673, 2088, 2754
AccB7I CCANNNNNTGG 2 cut(s) 2311, 2916
AccI GTMKAC 1 cut(s) 2515
AccII CGCG 5 cut(s) 167, 228, 454, 473, 2373
AccIII TCCGGA 1 cut(s) 3052
AcoI YGGCCR 2 cut(s) 237, 2313
AcsI RAATTY 4 cut(s) 595, 1408, 1822, 2961
AcuI CTGAAG 3 cut(s) 1038, 2513, 2525
AcvI CACGTG 1 cut(s) 3386
AcyI GRCGYC 1 cut(s) 410
AfaI GTAC 5 cut(s) 200, 350, 1620, 1735, 2579
AfeI AGCGCT 1 cut(s) 2671
AflIII ACRYGT 2 cut(s) 2717, 3383
AgeI ACCGGT 2 cut(s) 369, 447
AgsI TTSAA 8 cut(s) 1310, 1414, 2051, 2154, 2927, 2948, 3455, 3497
AhlI ACTAGT 1 cut(s) 932
AjnI CCWGG 6 cut(s) 1681, 1866, 2282, 2936, 3127, 3174
Alw21I GWGCWC 3 cut(s) 1532, 2693, 3553
Alw26I GTCTC 9 cut(s) 210, 324, 639, 758, 1064, 1178, 2264, 2986, 3543
AlwNI CAGNNNCTG 2 cut(s) 881, 1343
Ama87I CYCGRG 1 cut(s) 73
Aor13HI TCCGGA 1 cut(s) 3052
Aor51HI AGCGCT 1 cut(s) 2671
ApeKI GCWGC 7 cut(s) 468, 612, 2056, 2220, 2348, 2667, 3265
ApoI RAATTY 4 cut(s) 595, 1408, 1822, 2961
ArsI GACNNNNNNTTYG 8 cut(s) 278, 310, 668, 674, 700, 706, 1807, 1839
AsiGI ACCGGT 2 cut(s) 369, 447
Asp700I GAANNNNTTC 3 cut(s) 428, 2596, 3637
AspLEI GCGC 5 cut(s) 473, 475, 1888, 2400, 2672
AspS9I GGNCC 9 cut(s) 81, 208, 280, 445, 702, 1435, 1523, 3167, 3214
AsuC2I CCSGG 2 cut(s) 206, 212
AsuHPI GGTGA 6 cut(s) 274, 757, 926, 1007, 1129, 2109
AsuNHI GCTAGC 1 cut(s) 1888
AvaI CYCGRG 1 cut(s) 73
AvaII GGWCC 6 cut(s) 81, 208, 280, 445, 702, 1523
AxyI CCTNAGG 1 cut(s) 882
BaeGI GKGCMC 2 cut(s) 2091, 2482
BaeI ACNNNNGTAYC 2 cut(s) 340, 373
BalI TGGCCA 1 cut(s) 2315
BamHI GGATCC 1 cut(s) 46
BanI GGYRCC 4 cut(s) 1012, 1673, 2088, 2754
BanII GRGCYC 5 cut(s) 56, 786, 816, 966, 3553
BbrPI CACGTG 1 cut(s) 3386
BbsI GAAGAC 3 cut(s) 162, 2513, 2738
Bbv12I GWGCWC 3 cut(s) 1532, 2693, 3553
BbvCI CCTCAGC 3 cut(s) 1320, 3411, 3501
BbvI GCAGC 7 cut(s) 480, 624, 2068, 2232, 2360, 2679, 3277
BccI CCATC 9 cut(s) 253, 977, 983, 1088, 1461, 1739, 1822, 2481, 3577
BceAI ACGGC 3 cut(s) 349, 568, 3011
BcgI CGANNNNNNTGC 2 cut(s) 2815, 2849
BciT130I CCWGG 6 cut(s) 1683, 1868, 2284, 2938, 3129, 3176
BciVI GTATCC 1 cut(s) 3082
BclI TGATCA 3 cut(s) 1693, 2857, 3013
BcnI CCSGG 2 cut(s) 206, 212
BcoDI GTCTC 9 cut(s) 210, 324, 639, 758, 1064, 1178, 2264, 2986, 3543
BcuI ACTAGT 1 cut(s) 932
BfmI CTRYAG 3 cut(s) 2346, 2429, 3533
BfoI RGCGCY 2 cut(s) 1889, 2673
BfuI GTATCC 1 cut(s) 3082
BglI GCCNNNNNGGC 1 cut(s) 2477
BglII AGATCT 1 cut(s) 3601
BlpI GCTNAGC 1 cut(s) 2060
Bme1390I CCNGG 8 cut(s) 206, 212, 1683, 1868, 2284, 2938, 3129, 3176
Bme18I GGWCC 6 cut(s) 81, 208, 280, 445, 702, 1523
BmeT110I CYCGRG 1 cut(s) 73
BmgT120I GGNCC 9 cut(s) 81, 208, 280, 445, 702, 1435, 1523, 3167, 3214
BmrFI CCNGG 8 cut(s) 206, 212, 1683, 1868, 2284, 2938, 3129, 3176
BmsI GCATC 4 cut(s) 354, 2448, 2576, 2823
BmtI GCTAGC 1 cut(s) 1892
BpiI GAAGAC 3 cut(s) 162, 2513, 2738
BpmI CTGGAG 1 cut(s) 1389
Bpu10I CCTNAGC 4 cut(s) 840, 1320, 3411, 3501
Bpu1102I GCTNAGC 1 cut(s) 2060
BpuEI CTTGAG 7 cut(s) 980, 1643, 2124, 2602, 2802, 3104, 3545
BpuMI CCSGG 2 cut(s) 206, 212
BsaAI YACGTR 1 cut(s) 3386
BsaHI GRCGYC 1 cut(s) 410
BsaI GGTCTC 3 cut(s) 639, 758, 1178
BsaWI WCCGGW 5 cut(s) 369, 447, 576, 976, 3052
Bse118I RCCGGY 4 cut(s) 239, 274, 369, 447
Bse1I ACTGG 6 cut(s) 1372, 1951, 2300, 2515, 2815, 3438
Bse21I CCTNAGG 1 cut(s) 882
Bse3DI GCAATG 4 cut(s) 1707, 1957, 2277, 2771
BseAI TCCGGA 1 cut(s) 3052
BseBI CCWGG 6 cut(s) 1683, 1868, 2284, 2938, 3129, 3176
BseMI GCAATG 4 cut(s) 1707, 1957, 2277, 2771
BseNI ACTGG 6 cut(s) 1372, 1951, 2300, 2515, 2815, 3438
BsePI GCGCGC 1 cut(s) 471
BseSI GKGCMC 2 cut(s) 2091, 2482
BseX3I CGGCCG 1 cut(s) 237
BseXI GCAGC 7 cut(s) 480, 624, 2068, 2232, 2360, 2679, 3277
BseYI CCCAGC 1 cut(s) 3164
Bsh1236I CGCG 5 cut(s) 167, 228, 454, 473, 2373
Bsh1285I CGRYCG 8 cut(s) 205, 226, 240, 355, 374, 437, 452, 581
BshNI GGYRCC 4 cut(s) 1012, 1673, 2088, 2754
BshTI ACCGGT 2 cut(s) 369, 447
BsiEI CGRYCG 8 cut(s) 205, 226, 240, 355, 374, 437, 452, 581
BsiHKAI GWGCWC 3 cut(s) 1532, 2693, 3553
BsiHKCI CYCGRG 1 cut(s) 73
BsiWI CGTACG 1 cut(s) 198
BslFI GGGAC 4 cut(s) 221, 458, 1842, 2836
BsmAI GTCTC 9 cut(s) 210, 324, 639, 758, 1064, 1178, 2264, 2986, 3543
BsmBI CGTCTC 3 cut(s) 210, 324, 2986
BsmFI GGGAC 4 cut(s) 221, 458, 1842, 2836
BsmI GAATGC 1 cut(s) 2367
Bso31I GGTCTC 3 cut(s) 639, 758, 1178
BsoBI CYCGRG 1 cut(s) 73
Bsp1286I GDGCHC 9 cut(s) 56, 786, 816, 966, 1532, 2091, 2482, 2693, 3553
Bsp13I TCCGGA 1 cut(s) 3052
Bsp1720I GCTNAGC 1 cut(s) 2060
Bsp68I TCGCGA 2 cut(s) 228, 2373
BspEI TCCGGA 1 cut(s) 3052
BspFNI CGCG 5 cut(s) 167, 228, 454, 473, 2373
BspHI TCATGA 1 cut(s) 2326
BspMAI CTGCAG 3 cut(s) 2350, 2433, 3537
BspOI GCTAGC 1 cut(s) 1892
BspQI GCTCTTC 1 cut(s) 993
BspT107I GGYRCC 4 cut(s) 1012, 1673, 2088, 2754
BspTNI GGTCTC 3 cut(s) 639, 758, 1178
BsrDI GCAATG 4 cut(s) 1707, 1957, 2277, 2771
BsrFI RCCGGY 4 cut(s) 239, 274, 369, 447
BsrI ACTGG 6 cut(s) 1372, 1951, 2300, 2515, 2815, 3438
BssAI RCCGGY 4 cut(s) 239, 274, 369, 447
BssHII GCGCGC 1 cut(s) 471
BssNI GRCGYC 1 cut(s) 410
BssT1I CCWWGG 3 cut(s) 1283, 2451, 2910
Bst2UI CCWGG 6 cut(s) 1683, 1868, 2284, 2938, 3129, 3176
Bst4CI ACNGT 5 cut(s) 41, 1078, 1968, 3065, 3098
Bst6I CTCTTC 6 cut(s) 100, 157, 263, 746, 993, 1107
BstACI GRCGYC 1 cut(s) 410
BstBAI YACGTR 1 cut(s) 3386
BstEII GGTNACC 1 cut(s) 2115
BstENI CCTNNNNNAGG 2 cut(s) 1865, 3053
BstFNI CGCG 5 cut(s) 167, 228, 454, 473, 2373
BstH2I RGCGCY 2 cut(s) 1889, 2673
BstHHI GCGC 5 cut(s) 473, 475, 1888, 2400, 2672
BstMAI GTCTC 9 cut(s) 210, 324, 639, 758, 1064, 1178, 2264, 2986, 3543
BstMCI CGRYCG 8 cut(s) 205, 226, 240, 355, 374, 437, 452, 581
BstMWI GCNNNNNNNGC 8 cut(s) 185, 188, 387, 481, 2095, 2477, 2768, 3395
BstNI CCWGG 6 cut(s) 1683, 1868, 2284, 2938, 3129, 3176
BstNSI RCATGY 3 cut(s) 2355, 2404, 2721
BstPI GGTNACC 1 cut(s) 2115
BstSCI CCNGG 8 cut(s) 204, 210, 1681, 1866, 2282, 2936, 3127, 3174
BstSFI CTRYAG 3 cut(s) 2346, 2429, 3533
BstSLI GKGCMC 2 cut(s) 2091, 2482
BstUI CGCG 5 cut(s) 167, 228, 454, 473, 2373
BstV1I GCAGC 7 cut(s) 480, 624, 2068, 2232, 2360, 2679, 3277
BstV2I GAAGAC 3 cut(s) 162, 2513, 2738
BstX2I RGATCY 7 cut(s) 14, 25, 46, 2135, 2813, 3136, 3601
BstYI RGATCY 7 cut(s) 14, 25, 46, 2135, 2813, 3136, 3601
BstZI CGGCCG 1 cut(s) 237
Bsu36I CCTNAGG 1 cut(s) 882
BsuI GTATCC 1 cut(s) 3082
BtsI GCAGTG 4 cut(s) 2426, 2548, 2809, 3160
BtsIMutI CAGTG 8 cut(s) 1365, 1379, 2257, 2426, 2548, 2809, 3160, 3431
BtuMI TCGCGA 2 cut(s) 228, 2373
CaiI CAGNNNCTG 2 cut(s) 881, 1343
CciI TCATGA 1 cut(s) 2326
CfoI GCGC 5 cut(s) 473, 475, 1888, 2400, 2672
Cfr10I RCCGGY 4 cut(s) 239, 274, 369, 447
Cfr13I GGNCC 9 cut(s) 81, 208, 280, 445, 702, 1435, 1523, 3167, 3214
CseI GACGC 4 cut(s) 156, 399, 562, 2808
Csp6I GTAC 5 cut(s) 199, 349, 1619, 1734, 2578
CspAI ACCGGT 2 cut(s) 369, 447
CviQI GTAC 5 cut(s) 199, 349, 1619, 1734, 2578
DraI TTTAAA 1 cut(s) 652
EaeI YGGCCR 2 cut(s) 237, 2313
EagI CGGCCG 1 cut(s) 237
Eam1104I CTCTTC 6 cut(s) 100, 157, 263, 746, 993, 1107
EarI CTCTTC 6 cut(s) 100, 157, 263, 746, 993, 1107
EciI GGCGGA 4 cut(s) 261, 293, 357, 627
Ecl136II GAGCTC 1 cut(s) 3551
EclXI CGGCCG 1 cut(s) 237
Eco130I CCWWGG 3 cut(s) 1283, 2451, 2910
Eco24I GRGCYC 5 cut(s) 56, 786, 816, 966, 3553
Eco31I GGTCTC 3 cut(s) 639, 758, 1178
Eco47I GGWCC 6 cut(s) 81, 208, 280, 445, 702, 1523
Eco47III AGCGCT 1 cut(s) 2671
Eco52I CGGCCG 1 cut(s) 237
Eco53kI GAGCTC 1 cut(s) 3551
Eco57I CTGAAG 3 cut(s) 1038, 2513, 2525
Eco72I CACGTG 1 cut(s) 3386
Eco81I CCTNAGG 1 cut(s) 882
Eco88I CYCGRG 1 cut(s) 73
Eco91I GGTNACC 1 cut(s) 2115
EcoICRI GAGCTC 1 cut(s) 3551
EcoNI CCTNNNNNAGG 2 cut(s) 1865, 3053
EcoO109I RGGNCCY 1 cut(s) 3214
EcoO65I GGTNACC 1 cut(s) 2115
EcoRI GAATTC 2 cut(s) 595, 1822
EcoRII CCWGG 6 cut(s) 1681, 1866, 2282, 2936, 3127, 3174
EcoT14I CCWWGG 3 cut(s) 1283, 2451, 2910
EcoT38I GRGCYC 5 cut(s) 56, 786, 816, 966, 3553
ErhI CCWWGG 3 cut(s) 1283, 2451, 2910
Esp3I CGTCTC 3 cut(s) 210, 324, 2986
FalI AAGNNNNNCTT 4 cut(s) 942, 974, 1711, 1743
FaqI GGGAC 4 cut(s) 221, 458, 1842, 2836
FauI CCCGC 4 cut(s) 143, 386, 450, 2769
FbaI TGATCA 3 cut(s) 1693, 2857, 3013
FblI GTMKAC 1 cut(s) 2515
FriOI GRGCYC 5 cut(s) 56, 786, 816, 966, 3553
GlaI GCGC 5 cut(s) 472, 474, 1887, 2399, 2671
GsaI CCCAGC 1 cut(s) 3168
GsuI CTGGAG 1 cut(s) 1389
HaeII RGCGCY 2 cut(s) 1889, 2673
HgaI GACGC 4 cut(s) 156, 399, 562, 2808
HhaI GCGC 5 cut(s) 473, 475, 1888, 2400, 2672
Hin1I GRCGYC 1 cut(s) 410
Hin6I GCGC 5 cut(s) 471, 473, 1886, 2398, 2670
HinP1I GCGC 5 cut(s) 471, 473, 1886, 2398, 2670
HindIII AAGCTT 3 cut(s) 106, 1645, 2062
HphI GGTGA 6 cut(s) 274, 757, 926, 1007, 1129, 2109
Hpy166II GTNNAC 4 cut(s) 1273, 1551, 2516, 3061
Hpy8I GTNNAC 4 cut(s) 1273, 1551, 2516, 3061
Hpy99I CGWCG 5 cut(s) 224, 290, 415, 589, 670
HpyCH4III ACNGT 5 cut(s) 41, 1078, 1968, 3065, 3098
HpyCH4IV ACGT 1 cut(s) 3385
HpyF10VI GCNNNNNNNGC 8 cut(s) 185, 188, 387, 481, 2095, 2477, 2768, 3395
HpySE526I ACGT 1 cut(s) 3385
Hsp92I GRCGYC 1 cut(s) 410
HspAI GCGC 5 cut(s) 471, 473, 1886, 2398, 2670
Kpn2I TCCGGA 1 cut(s) 3052
KroI GCCGGC 2 cut(s) 239, 274
KroNI GCCGGC 2 cut(s) 241, 276
Ksp22I TGATCA 3 cut(s) 1693, 2857, 3013
LguI GCTCTTC 1 cut(s) 993
Lsp1109I GCAGC 7 cut(s) 480, 624, 2068, 2232, 2360, 2679, 3277
LweI GCATC 4 cut(s) 354, 2448, 2576, 2823
MaeII ACGT 1 cut(s) 3385
MaeIII GTNAC 6 cut(s) 1354, 1662, 1721, 1975, 2115, 3065
MfeI CAATTG 1 cut(s) 2483
MflI RGATCY 7 cut(s) 14, 25, 46, 2135, 2813, 3136, 3601
MhlI GDGCHC 9 cut(s) 56, 786, 816, 966, 1532, 2091, 2482, 2693, 3553
MlsI TGGCCA 1 cut(s) 2315
MluNI TGGCCA 1 cut(s) 2315
MlyI GAGTC 4 cut(s) 1349, 1396, 2617, 3296
MmeI TCCRAC 9 cut(s) 354, 393, 519, 1245, 2113, 2585, 2705, 3204, 3315
Mox20I TGGCCA 1 cut(s) 2315
MroI TCCGGA 1 cut(s) 3052
MroNI GCCGGC 2 cut(s) 239, 274
MroXI GAANNNNTTC 3 cut(s) 428, 2596, 3637
MscI TGGCCA 1 cut(s) 2315
MslI CAYNNNNRTG 1 cut(s) 2106
Msp20I TGGCCA 1 cut(s) 2315
MspR9I CCNGG 8 cut(s) 206, 212, 1683, 1868, 2284, 2938, 3129, 3176
MunI CAATTG 1 cut(s) 2483
Mva1269I GAATGC 1 cut(s) 2367
MvaI CCWGG 6 cut(s) 1683, 1868, 2284, 2938, 3129, 3176
MvnI CGCG 5 cut(s) 167, 228, 454, 473, 2373
MwoI GCNNNNNNNGC 8 cut(s) 185, 188, 387, 481, 2095, 2477, 2768, 3395
NaeI GCCGGC 2 cut(s) 241, 276
NciI CCSGG 2 cut(s) 206, 212
NgoMIV GCCGGC 2 cut(s) 239, 274
NheI GCTAGC 1 cut(s) 1888
NmeAIII GCCGAG 4 cut(s) 666, 913, 1070, 3388
NmuCI GTSAC 2 cut(s) 1354, 2115
NruI TCGCGA 2 cut(s) 228, 2373
NspI RCATGY 3 cut(s) 2355, 2404, 2721
PaeI GCATGC 1 cut(s) 2404
PagI TCATGA 1 cut(s) 2326
PauI GCGCGC 1 cut(s) 471
PciI ACATGT 1 cut(s) 2717
PciSI GCTCTTC 1 cut(s) 993
PcsI WCGNNNNNNNCGW 5 cut(s) 225, 419, 671, 1259, 3553
PctI GAATGC 1 cut(s) 2367
PdiI GCCGGC 2 cut(s) 241, 276
PdmI GAANNNNTTC 3 cut(s) 428, 2596, 3637
PfeI GAWTC 9 cut(s) 32, 605, 710, 1384, 1448, 1481, 1586, 2780, 3227
Pfl23II CGTACG 1 cut(s) 198
PflFI GACNNNGTC 2 cut(s) 700, 1339
PflMI CCANNNNNTGG 2 cut(s) 2311, 2916
PinAI ACCGGT 2 cut(s) 369, 447
Ple19I CGATCG 3 cut(s) 226, 355, 437
PleI GAGTC 4 cut(s) 1348, 1396, 2616, 3295
PmaCI CACGTG 1 cut(s) 3386
PmlI CACGTG 1 cut(s) 3386
PpsI GAGTC 4 cut(s) 1348, 1396, 2616, 3295
Ppu21I YACGTR 1 cut(s) 3386
PscI ACATGT 1 cut(s) 2717
Psp124BI GAGCTC 1 cut(s) 3553
Psp6I CCWGG 6 cut(s) 1681, 1866, 2282, 2936, 3127, 3174
PspCI CACGTG 1 cut(s) 3386
PspEI GGTNACC 1 cut(s) 2115
PspFI CCCAGC 1 cut(s) 3164
PspGI CCWGG 6 cut(s) 1681, 1866, 2282, 2936, 3127, 3174
PspLI CGTACG 1 cut(s) 198
PspPI GGNCC 9 cut(s) 81, 208, 280, 445, 702, 1435, 1523, 3167, 3214
PstI CTGCAG 3 cut(s) 2350, 2433, 3537
PstNI CAGNNNCTG 2 cut(s) 881, 1343
PsuI RGATCY 7 cut(s) 14, 25, 46, 2135, 2813, 3136, 3601
PsyI GACNNNGTC 2 cut(s) 700, 1339
PteI GCGCGC 1 cut(s) 471
PvuI CGATCG 3 cut(s) 226, 355, 437
RruI TCGCGA 2 cut(s) 228, 2373
RsaI GTAC 5 cut(s) 200, 350, 1620, 1735, 2579
RsaNI GTAC 5 cut(s) 199, 349, 1619, 1734, 2578
RseI CAYNNNNRTG 1 cut(s) 2106
SacI GAGCTC 1 cut(s) 3553
SapI GCTCTTC 1 cut(s) 993
Sau96I GGNCC 9 cut(s) 81, 208, 280, 445, 702, 1435, 1523, 3167, 3214
SchI GAGTC 4 cut(s) 1349, 1396, 2617, 3296
ScrFI CCNGG 8 cut(s) 206, 212, 1683, 1868, 2284, 2938, 3129, 3176
SduI GDGCHC 9 cut(s) 56, 786, 816, 966, 1532, 2091, 2482, 2693, 3553
SfaNI GCATC 4 cut(s) 354, 2448, 2576, 2823
SfcI CTRYAG 3 cut(s) 2346, 2429, 3533
SinI GGWCC 6 cut(s) 81, 208, 280, 445, 702, 1523
SmiMI CAYNNNNRTG 1 cut(s) 2106
SmlI CTYRAG 7 cut(s) 959, 1622, 2139, 2581, 2817, 3083, 3524
SmoI CTYRAG 7 cut(s) 959, 1622, 2139, 2581, 2817, 3083, 3524
SpeI ACTAGT 1 cut(s) 932
SphI GCATGC 1 cut(s) 2404
SspI AATATT 2 cut(s) 2493, 3563
SstI GAGCTC 1 cut(s) 3553
StyD4I CCNGG 8 cut(s) 204, 210, 1681, 1866, 2282, 2936, 3127, 3174
StyI CCWWGG 3 cut(s) 1283, 2451, 2910
TaaI ACNGT 5 cut(s) 41, 1078, 1968, 3065, 3098
TaiI ACGT 1 cut(s) 3388
TaqII GACCGA 2 cut(s) 782, 1540
TatI WGTACW 1 cut(s) 2577
TfiI GAWTC 9 cut(s) 32, 605, 710, 1384, 1448, 1481, 1586, 2780, 3227
TscAI CASTG 8 cut(s) 1372, 1386, 2257, 2433, 2548, 2809, 3160, 3438
TseFI GTSAC 2 cut(s) 1354, 2115
TseI GCWGC 7 cut(s) 468, 612, 2056, 2220, 2348, 2667, 3265
Tsp45I GTSAC 2 cut(s) 1354, 2115
TspDTI ATGAA 9 cut(s) 17, 1364, 1616, 2315, 2343, 2640, 2738, 2831, 3587
TspGWI ACGGA 3 cut(s) 303, 1082, 3476
TspRI CASTG 8 cut(s) 1372, 1386, 2257, 2433, 2548, 2809, 3160, 3438
Tth111I GACNNNGTC 2 cut(s) 700, 1339
Van91I CCANNNNNTGG 2 cut(s) 2311, 2916
VpaK11BI GGWCC 6 cut(s) 81, 208, 280, 445, 702, 1523
XagI CCTNNNNNAGG 2 cut(s) 1865, 3053
XapI RAATTY 4 cut(s) 595, 1408, 1822, 2961
XbaI TCTAGA 1 cut(s) 3541
XceI RCATGY 3 cut(s) 2355, 2404, 2721
XmiI GTMKAC 1 cut(s) 2515
XmnI GAANNNNTTC 3 cut(s) 428, 2596, 3637
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.