Rmu_sc0034555.1_g000001

RNA helicase SDE3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0034555.1
OG0000595
Physical Location & Seq
Forward (+)
1 .. 1378
1378 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0034555.1_g000001.1.cds

Sequence Viewer

Length: 1185 bp
ggcttcatctataaagttgttgggagcaaacgcgttttggttgaatttgaagatggcttttattctaatcatcattccaacaaaaaatatgatatcagcttctcattcaatagagtttgtttgaaaagagcccaccatgcagttaaagctgcatcagacgccttgtttcagaacttcctcttccctgacactgactcacgagcaagcactcctactgcaccagctctgctttctactacctgtcataagcttgatcaagatcaacgttgtgcggttggacatatcttaaggattcagggctcaccaccttatgtagtaactggtccggactgtgagggcgaatcttttgcatgtttaagagaaccttcaaaaactgaaggcaaatcttttgcatatttaagagaaccttcaaaaactggagtggttgttagtgaagcagtatatcaattatgtcagaagtcgccaaagtaccggattcttatctgtgcgcctactaacagctgctgcgatgtgctgatgagaagcttggtgaaggtgattccagagtcaactatgtttcgtgccaatgctgcattccgtgagaaagaagaggtacctgaggatatcctccgctcatccctttacaaggagtcttgtttttcttgtccttcaatagagaaactccggaaatacagggtgattttctcgactttcatgagtagctttcgactacatgataaaggcatagctgttggacattttagccatatttttctggtggatgcttcatccgctattgaaccggaaacattggtcgccctgactaactttgctgacaagactactagtgttatagttactggtaaacctggaaacagtccgcgctgggttcgcgctgacattgcaataaactccggaaatacaggccatatttttctggtggatgcttcatccgctattgaaccagaaacattggtcgccctgaccaactttgctgacaagactactagtgttatagttactggtaaacctggaaacagtccgcgctgggttcgcgctgacattgcaaggaaaaagggactcaagatttcatactttgaaagactctgtaagctcagcccctatagaagccgcagtgcgatgttcatcacacaattagacctgaaccaaaagtcaggctcagactaccaaacaagaactttctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

394

Amino Acids

43.86

Weight (kDa)

9.16

Isoelectric Point (pI)

45.87

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000595)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g37100 FvH4_4g37100 FvH4_4g37100
malus_domestica MD13G1238300.v1.1 MD16G1243100.v1.1 MD16G1243200.v1.1
prunus_persica Prupe.1G070100_v2.0.a1 Prupe.1G070200_v2.0.a1 Prupe.1G070300_v2.0.a1 Prupe.1G070300_v2.0.a1
pyrus_communis pycom13g21070 pycom16g20390 pycom16g20400
rosa_chinensis RchiOBHm_Chr1g0318071 RchiOBHm_Chr1g0318111 RchiOBHm_Chr4g0446861 RchiOBHm_Chr4g0446881 RchiOBHm_Chr4g0446891
rosa_laevigata RLG00000005623 RLG00000005624 RLG00000009337 RLG00000030560 RLG00000030566
rosa_multiflora Rmu_co8177450.1_g000001 Rmu_sc0001753.1_g000012 Rmu_sc0001753.1_g000013 Rmu_sc0001753.1_g000014 Rmu_sc0001753.1_g000015 Rmu_sc0011424.1_g000009 Rmu_sc0011424.1_g000020 Rmu_sc0034555.1_g000001 Rmu_ssc0000008.1_g000002
rosa_roxburghii Rroxscaffold_4G00329530 Rroxscaffold_4G00329590 Rroxscaffold_4G00329800 Rroxscaffold_4G00329810 Rroxscaffold_4G00329860 Rroxscaffold_4G00329910 Rroxscaffold_4G00330030 Rroxscaffold_5G00343610 Rroxscaffold_5G00348280 Rroxscaffold_5G00387220 Rroxscaffold_5G00387230
rosa_rugosa Rorug01G0019100 Rorug01G0019100 Rorug01G0019500 Rorug01G0019600 Rorug04G0010100 Rorug04G0375200 Rorug04G0375300 Rorug04G0375400 Rorug04G0375500 Rorug04G0375600 Rorug04G0375700
rosa_samantha Rh1AG030700 Rh1AG030900 Rh1BG026900 Rh1CG029500 Rh1CG029700 Rh1DG040500 Rh4AG088100 Rh4AG436700 Rh4AG436800 Rh4AG436900 Rh4BG084700 Rh4BG445800 Rh4BG445900 Rh4BG446100 Rh4CG096300 Rh4CG463100 Rh4CG463200 Rh4CG463400 Rh4DG444500 Rh4DG444600 Rh4DG444700
rosa_wichuraiana Rw1G002420 Rw1G002440 Rw1G002480 Rw4G037360 Rw4G037370 Rw4G037380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 592
AccB1I GGYRCC 1 cut(s) 592
AccBSI CCGCTC 1 cut(s) 612
AccII CGCG 5 cut(s) 33, 862, 873, 1024, 1035
AccIII TCCGGA 3 cut(s) 325, 663, 893
AciI CCGC 7 cut(s) 272, 610, 771, 860, 933, 1022, 1111
AclI AACGTT 1 cut(s) 265
AcsI RAATTY 1 cut(s) 44
AcuI CTGAAG 1 cut(s) 396
AcyI GRCGYC 1 cut(s) 159
AfaI GTAC 2 cut(s) 470, 594
AfiI CCNNNNNNNGG 1 cut(s) 625
AflII CTTAAG 1 cut(s) 286
AflIII ACRYGT 1 cut(s) 31
AhlI ACTAGT 2 cut(s) 824, 986
AjnI CCWGG 2 cut(s) 847, 1009
AjuI GAANNNNNNNTTGG 2 cut(s) 557, 589
AluBI AGCT 9 cut(s) 99, 149, 224, 250, 501, 525, 702, 728, 1093
AluI AGCT 9 cut(s) 99, 149, 224, 250, 501, 525, 702, 728, 1093
AlwNI CAGNNNCTG 1 cut(s) 504
Aor13HI TCCGGA 3 cut(s) 325, 663, 893
AoxI GGCC 1 cut(s) 904
ApeKI GCWGC 4 cut(s) 149, 501, 504, 569
ApoI RAATTY 1 cut(s) 44
Asp718I GGTACC 1 cut(s) 592
AspLEI GCGC 5 cut(s) 490, 864, 875, 1026, 1037
AspS9I GGNCC 1 cut(s) 323
AsuHPI GGTGA 4 cut(s) 294, 541, 547, 688
AvaII GGWCC 1 cut(s) 323
AxyI CCTNAGG 1 cut(s) 597
BanI GGYRCC 1 cut(s) 592
BanII GRGCYC 2 cut(s) 133, 302
BauI CACGAG 1 cut(s) 198
BbvI GCAGC 4 cut(s) 136, 488, 491, 556
BccI CCATC 1 cut(s) 47
BcgI CGANNNNNNTGC 2 cut(s) 329, 363
BciT130I CCWGG 2 cut(s) 849, 1011
BclI TGATCA 1 cut(s) 253
BcuI ACTAGT 2 cut(s) 824, 986
BfaI CTAG 2 cut(s) 825, 987
BfmI CTRYAG 1 cut(s) 1102
BfrI CTTAAG 1 cut(s) 286
BisI GCNGC 5 cut(s) 150, 502, 505, 570, 1111
BlpI GCTNAGC 1 cut(s) 1094
BlsI GCNGC 5 cut(s) 151, 503, 506, 571, 1112
Bme1390I CCNGG 2 cut(s) 849, 1011
Bme18I GGWCC 1 cut(s) 323
BmgT120I GGNCC 1 cut(s) 323
BmiI GGNNCC 1 cut(s) 594
BmrFI CCNGG 2 cut(s) 849, 1011
BmsI GCATC 3 cut(s) 161, 751, 913
BpmI CTGGAG 1 cut(s) 438
Bpu1102I GCTNAGC 1 cut(s) 1094
BpuEI CTTGAG 1 cut(s) 1046
BsaBI GATNNNNATC 3 cut(s) 258, 479, 1124
BsaHI GRCGYC 1 cut(s) 159
BsaWI WCCGGW 5 cut(s) 325, 471, 663, 781, 893
BsaXI ACNNNNNCTCC 2 cut(s) 411, 441
Bsc4I CCNNNNNNNGG 1 cut(s) 625
Bse1I ACTGG 4 cut(s) 325, 421, 844, 1006
Bse21I CCTNAGG 1 cut(s) 597
Bse3DI GCAATG 2 cut(s) 879, 1041
Bse8I GATNNNNATC 3 cut(s) 258, 479, 1124
BseAI TCCGGA 3 cut(s) 325, 663, 893
BseBI CCWGG 2 cut(s) 849, 1011
BseGI GGATG 5 cut(s) 614, 766, 767, 928, 929
BseJI GATNNNNATC 3 cut(s) 258, 479, 1124
BseLI CCNNNNNNNGG 1 cut(s) 625
BseMI GCAATG 2 cut(s) 879, 1041
BseMII CTCAG 3 cut(s) 588, 1108, 1173
BseNI ACTGG 4 cut(s) 325, 421, 844, 1006
BseXI GCAGC 4 cut(s) 136, 488, 491, 556
BseYI CCCAGC 2 cut(s) 864, 1026
BsgI GTGCAG 1 cut(s) 201
Bsh1236I CGCG 5 cut(s) 33, 862, 873, 1024, 1035
BshFI GGCC 1 cut(s) 906
BshNI GGYRCC 1 cut(s) 592
BsiSI CCGG 5 cut(s) 326, 472, 664, 782, 894
BslFI GGGAC 1 cut(s) 1071
BslI CCNNNNNNNGG 1 cut(s) 625
BsmFI GGGAC 1 cut(s) 1071
BsmI GAATGC 1 cut(s) 572
BsnI GGCC 1 cut(s) 906
Bsp1286I GDGCHC 2 cut(s) 133, 302
Bsp13I TCCGGA 3 cut(s) 325, 663, 893
Bsp143I GATC 2 cut(s) 253, 259
Bsp1720I GCTNAGC 1 cut(s) 1094
BspACI CCGC 7 cut(s) 272, 610, 771, 860, 933, 1022, 1111
BspANI GGCC 1 cut(s) 906
BspCNI CTCAG 3 cut(s) 589, 1107, 1172
BspEI TCCGGA 3 cut(s) 325, 663, 893
BspFNI CGCG 5 cut(s) 33, 862, 873, 1024, 1035
BspHI TCATGA 1 cut(s) 693
BspLI GGNNCC 1 cut(s) 594
BspT107I GGYRCC 1 cut(s) 592
BspTI CTTAAG 1 cut(s) 286
BsrBI CCGCTC 1 cut(s) 612
BsrDI GCAATG 2 cut(s) 879, 1041
BsrI ACTGG 4 cut(s) 325, 421, 844, 1006
BssMI GATC 2 cut(s) 253, 259
BssNI GRCGYC 1 cut(s) 159
BssSI CACGAG 1 cut(s) 198
Bst2BI CACGAG 1 cut(s) 198
Bst2UI CCWGG 2 cut(s) 849, 1011
Bst4CI ACNGT 3 cut(s) 332, 857, 1019
Bst6I CTCTTC 2 cut(s) 185, 582
BstACI GRCGYC 1 cut(s) 159
BstAFI CTTAAG 1 cut(s) 286
BstC8I GCNNGC 1 cut(s) 205
BstDEI CTNAG 3 cut(s) 597, 1094, 1159
BstENI CCTNNNNNAGG 1 cut(s) 623
BstF5I GGATG 5 cut(s) 614, 766, 767, 928, 929
BstFNI CGCG 5 cut(s) 33, 862, 873, 1024, 1035
BstHHI GCGC 5 cut(s) 490, 864, 875, 1026, 1037
BstKTI GATC 2 cut(s) 256, 262
BstMBI GATC 2 cut(s) 253, 259
BstNI CCWGG 2 cut(s) 849, 1011
BstNSI RCATGY 1 cut(s) 354
BstSCI CCNGG 2 cut(s) 847, 1009
BstSFI CTRYAG 1 cut(s) 1102
BstUI CGCG 5 cut(s) 33, 862, 873, 1024, 1035
BstV1I GCAGC 4 cut(s) 136, 488, 491, 556
Bsu36I CCTNAGG 1 cut(s) 597
BsuRI GGCC 1 cut(s) 906
BtgZI GCGATG 2 cut(s) 522, 1133
BtsCI GGATG 5 cut(s) 614, 766, 767, 928, 929
BtsI GCAGTG 1 cut(s) 1120
BtsIMutI CAGTG 2 cut(s) 189, 1120
Cac8I GCNNGC 1 cut(s) 205
CaiI CAGNNNCTG 1 cut(s) 504
CciI TCATGA 1 cut(s) 693
CfoI GCGC 5 cut(s) 490, 864, 875, 1026, 1037
Cfr13I GGNCC 1 cut(s) 323
CseI GACGC 1 cut(s) 167
Csp6I GTAC 2 cut(s) 469, 593
CviAII CATG 4 cut(s) 137, 351, 694, 713
CviQI GTAC 2 cut(s) 469, 593
DdeI CTNAG 3 cut(s) 597, 1094, 1159
DpnI GATC 2 cut(s) 255, 261
DpnII GATC 2 cut(s) 253, 259
Eam1104I CTCTTC 2 cut(s) 185, 582
EarI CTCTTC 2 cut(s) 185, 582
Eco24I GRGCYC 2 cut(s) 133, 302
Eco32I GATATC 2 cut(s) 94, 604
Eco47I GGWCC 1 cut(s) 323
Eco57I CTGAAG 1 cut(s) 396
Eco81I CCTNAGG 1 cut(s) 597
EcoNI CCTNNNNNAGG 1 cut(s) 623
EcoRII CCWGG 2 cut(s) 847, 1009
EcoRV GATATC 2 cut(s) 94, 604
EcoT38I GRGCYC 2 cut(s) 133, 302
FaeI CATG 4 cut(s) 140, 354, 697, 716
FalI AAGNNNNNCTT 4 cut(s) 349, 381, 391, 423
FaqI GGGAC 1 cut(s) 1071
FatI CATG 4 cut(s) 136, 350, 693, 712
FbaI TGATCA 1 cut(s) 253
Fnu4HI GCNGC 5 cut(s) 150, 502, 505, 570, 1111
FokI GGATG 5 cut(s) 601, 754, 773, 916, 935
FriOI GRGCYC 2 cut(s) 133, 302
Fsp4HI GCNGC 5 cut(s) 150, 502, 505, 570, 1111
FspBI CTAG 2 cut(s) 825, 987
GlaI GCGC 5 cut(s) 489, 863, 874, 1025, 1036
GluI GCNGC 5 cut(s) 150, 502, 505, 570, 1111
GsaI CCCAGC 2 cut(s) 868, 1030
GsuI CTGGAG 1 cut(s) 438
HaeIII GGCC 1 cut(s) 906
HapII CCGG 5 cut(s) 326, 472, 664, 782, 894
HgaI GACGC 1 cut(s) 167
HhaI GCGC 5 cut(s) 490, 864, 875, 1026, 1037
Hin1I GRCGYC 1 cut(s) 159
Hin1II CATG 4 cut(s) 140, 354, 697, 716
Hin6I GCGC 5 cut(s) 488, 862, 873, 1024, 1035
HinP1I GCGC 5 cut(s) 488, 862, 873, 1024, 1035
HincII GTYRAC 1 cut(s) 549
HindII GTYRAC 1 cut(s) 549
HindIII AAGCTT 2 cut(s) 248, 523
HinfI GANTC 9 cut(s) 194, 292, 341, 475, 538, 545, 629, 1059, 1083
HpaII CCGG 5 cut(s) 326, 472, 664, 782, 894
HphI GGTGA 4 cut(s) 294, 541, 547, 688
Hpy166II GTNNAC 3 cut(s) 549, 845, 1007
Hpy188I TCNGA 5 cut(s) 157, 171, 456, 1162, 1184
Hpy188III TCNNGA 9 cut(s) 198, 257, 326, 542, 664, 685, 694, 894, 1063
Hpy8I GTNNAC 3 cut(s) 549, 845, 1007
HpyAV CCTTC 5 cut(s) 371, 375, 417, 526, 657
HpyCH4III ACNGT 3 cut(s) 332, 857, 1019
HpyCH4IV ACGT 1 cut(s) 265
HpyCH4V TGCA 8 cut(s) 140, 152, 218, 350, 392, 572, 884, 1046
HpyF3I CTNAG 3 cut(s) 597, 1094, 1159
HpySE526I ACGT 1 cut(s) 265
Hsp92I GRCGYC 1 cut(s) 159
Hsp92II CATG 4 cut(s) 140, 354, 697, 716
HspAI GCGC 5 cut(s) 488, 862, 873, 1024, 1035
Kpn2I TCCGGA 3 cut(s) 325, 663, 893
KpnI GGTACC 1 cut(s) 596
Ksp22I TGATCA 1 cut(s) 253
Kzo9I GATC 2 cut(s) 253, 259
LmnI GCTCC 1 cut(s) 24
Lsp1109I GCAGC 4 cut(s) 136, 488, 491, 556
LweI GCATC 3 cut(s) 161, 751, 913
MaeI CTAG 2 cut(s) 825, 987
MaeII ACGT 1 cut(s) 265
MaeIII GTNAC 3 cut(s) 316, 835, 997
MalI GATC 2 cut(s) 255, 261
MbiI CCGCTC 1 cut(s) 612
MboI GATC 2 cut(s) 253, 259
MboII GAAGA 3 cut(s) 62, 172, 599
MhlI GDGCHC 2 cut(s) 133, 302
MluCI AATT 3 cut(s) 44, 446, 1133
MluI ACGCGT 1 cut(s) 31
MlyI GAGTC 5 cut(s) 188, 554, 638, 1053, 1077
MmeI TCCRAC 3 cut(s) 102, 256, 712
MnlI CCTC 5 cut(s) 188, 328, 583, 592, 617
MroI TCCGGA 3 cut(s) 325, 663, 893
MseI TTAA 4 cut(s) 144, 287, 356, 398
MspA1I CMGCKG 1 cut(s) 501
MspCI CTTAAG 1 cut(s) 286
MspI CCGG 5 cut(s) 326, 472, 664, 782, 894
MspR9I CCNGG 2 cut(s) 849, 1011
Mva1269I GAATGC 1 cut(s) 572
MvaI CCWGG 2 cut(s) 849, 1011
MvnI CGCG 5 cut(s) 33, 862, 873, 1024, 1035
NdeII GATC 2 cut(s) 253, 259
NlaIII CATG 4 cut(s) 140, 354, 697, 716
NlaIV GGNNCC 1 cut(s) 594
NspI RCATGY 1 cut(s) 354
PagI TCATGA 1 cut(s) 693
PctI GAATGC 1 cut(s) 572
PfeI GAWTC 4 cut(s) 292, 341, 475, 538
PkrI GCNGC 5 cut(s) 151, 503, 506, 571, 1112
PleI GAGTC 5 cut(s) 188, 553, 637, 1053, 1077
PpsI GAGTC 5 cut(s) 188, 553, 637, 1053, 1077
Psp1406I AACGTT 1 cut(s) 265
Psp6I CCWGG 2 cut(s) 847, 1009
PspFI CCCAGC 2 cut(s) 864, 1026
PspGI CCWGG 2 cut(s) 847, 1009
PspN4I GGNNCC 1 cut(s) 594
PspPI GGNCC 1 cut(s) 323
PstNI CAGNNNCTG 1 cut(s) 504
PvuII CAGCTG 1 cut(s) 501
RsaI GTAC 2 cut(s) 470, 594
RsaNI GTAC 2 cut(s) 469, 593
SaqAI TTAA 4 cut(s) 144, 287, 356, 398
SatI GCNGC 5 cut(s) 150, 502, 505, 570, 1111
Sau3AI GATC 2 cut(s) 253, 259
Sau96I GGNCC 1 cut(s) 323
SchI GAGTC 5 cut(s) 188, 554, 638, 1053, 1077
ScrFI CCNGG 2 cut(s) 849, 1011
SduI GDGCHC 2 cut(s) 133, 302
SfaNI GCATC 3 cut(s) 161, 751, 913
SfcI CTRYAG 1 cut(s) 1102
SinI GGWCC 1 cut(s) 323
SmlI CTYRAG 2 cut(s) 286, 1061
SmoI CTYRAG 2 cut(s) 286, 1061
SpeI ACTAGT 2 cut(s) 824, 986
Sse9I AATT 3 cut(s) 44, 446, 1133
SsiI CCGC 7 cut(s) 272, 610, 771, 860, 933, 1022, 1111
SspMI CTAG 2 cut(s) 825, 987
StyD4I CCNGG 2 cut(s) 847, 1009
TaaI ACNGT 3 cut(s) 332, 857, 1019
TaiI ACGT 1 cut(s) 268
TaqI TCGA 2 cut(s) 686, 706
TasI AATT 3 cut(s) 44, 446, 1133
TauI GCSGC 1 cut(s) 1113
TfiI GAWTC 4 cut(s) 292, 341, 475, 538
Tru1I TTAA 4 cut(s) 144, 287, 356, 398
Tru9I TTAA 4 cut(s) 144, 287, 356, 398
TscAI CASTG 2 cut(s) 196, 1120
TseI GCWGC 4 cut(s) 149, 501, 504, 569
TspDTI ATGAA 5 cut(s) 682, 756, 918, 1059, 1114
TspGWI ACGGA 1 cut(s) 566
TspRI CASTG 2 cut(s) 196, 1120
Vha464I CTTAAG 1 cut(s) 286
VpaK11BI GGWCC 1 cut(s) 323
XagI CCTNNNNNAGG 1 cut(s) 623
XapI RAATTY 1 cut(s) 44
XceI RCATGY 1 cut(s) 354
XspI CTAG 2 cut(s) 825, 987
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.