RLG00000030560

RNA helicase SDE3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0000595
Physical Location & Seq
Forward (+)
63499345 .. 63502264
2920 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000030560

Sequence Viewer

Length: 2193 bp
ATGATGAGGTTGGGGATAATCATTGGGGCTTCAGCAATATTATTGTGGGAATACTTTGAAAATAAGAGACCCACACCATCATCATCATCCCCTGAAACGCCACTTCAGCGGCAGCCAACGTCATCTAACTCTAAACCACATCCAGCCTCCGCAGAACCCCCACCATATTATTCTAAACCATCTCCATCTCCATCTTCCCCGAAACCCCCACCATATTCTTCTAAACCACCTTCATCTCCAGCTTCTTCTAAACCACCTTCATCCCCAGCTTCTTCTAAACCACCTGCATCCCCAATTTCTTCTAAACCACCTTCATCTCCAGCTTCTTCTAAACCACCTTCATCCCCAGCTTCTTCTAAACCACCTGCATCCCCAATTTCTTCTAAACCACCTTCATCCCCAGCTTCTTCTAAACCACCTTCATCCCCAGCTTCTTCTAAACCACCTTCATCCCCAGCTTCTTCTCCCCCACCATATTCTTCTAAACCACCTTCATCCCTAGCTTCTTCTAAACCACCTTCATCTGCAGCTTCCCCACAACCCCCAGCATATTCTTCTAAACCACCTGCATCCCCAGCATGTTCTTCTAAACCACCTTCATCTCCAGTTTCCCCACAACCCCCAGCATATTCTTCTAAACCATCTTCATCTCCATCTTCCCCAAAACACCCTCCAGCACATTTTTCTATACCATCTCCATCTCCACCTCCCTCTCCCCCAAAGTGCCCTCCTGTCTTTAAGTCAGTTCTACGCCCAGCCTCCTCTTCCTCAAATAATGATGATATAAGTCAAAACCAACAATATGGGAAGAGAACAGCTTATGTGTGTGTTGAAGAGGATCCATTACCTGTATTCATGATTCCTGAGGATGTCGAAGCCTTGATCAAGAAAGAAATTGCGCCCAAAGTTCTGAATCGCCCATTATCTCCCACAACATACAAGGACAACTTTGCCGCTCTCTTATATTCTGAAGATTTCTACTTGGAGAAATGGAGTGATTTCCTTTTGAAGGGTGTGACATTAGCGTTGGATGAAAGTTCAATTTATAAAAACCGGAAAAAGGAAGCACTTTATAAAAATCGGAAAAAGGAAGACAAGGAAGACAAAACCTTTGTAGCATTTGAGCTTGATCGTGTTCCTGAGGAACGACCATTCCTCTTGTCAAGGGACTTGGTCCTTGCACGACCAGTGGGGAAGAGTTCTACTGAGCCCTTTAAGGGCCTCATCTATAAAGTTGTTGGGAGCAATCGCGTTTTGGTTGAATTTGAGGATGACTTTTATTCTTATCATCATTCCAACAAAAAATATGATATCAGCTTCTCATTCAATAGAGTTTGTTTGAAAAGAGCCCACCATGCAGTAAAAGCTGCATCAGATGCCTTGTTTCAGAACTTCCTCTTCCCTGACTGTGACTCACGAGCAAGCACTCCTACTGCAACAGCTCTGCTTTCTACTACCTGTCATAAGCTTGATCAAGATCAACGTTCTGCAGTCGGACATATCTTAAGGATTCAGGGCTCACCACCTTATGTAGTAACTGGTCCGGACTGTGAAGCCAAATCTTTTGCATATTTAAGAGAACCTTCAAGAACTGGAGTGATTGTTAGTGAAGCAGTATACCAATTATGTCAGAAGTCACCAGAGTATCGCATTCTTATCTGTGCACCTACTAACAGCTGCTGCGATATGCTGATGAGAAGCTTGGTGAAGGTGATTCCAGAGTCGACTATGTTTCGTGCCAATGCTGCATTCCGAGAGAGAGATGAGGTACCTGAGGACATCCTCCGCTCATCCCTTTACAAAGAGTCTTGTTTTTCTTGTCCTGCAATAGAGAAACTCCGGGAATACAAGGTGATTTTCTCAACTTTCGTGAGTAGCTTCCGACTACATGATAAAGGCATAGCTGCTGGACATTTTAGCCATATTTTTCTGGTGGATGCTTCATCTACTATTGAGCCGGAAACATTGGTCGCCCTGACTAACTTCGCTGTCAAGGCCACTACTGTTATAGTTACTGGTAATCATGGAAATAGTCCGCTCTGGGTTCGCGCTGACATTGCAAGGAAAAAGGGACTCAAGATTTCCTACTTTGAACGACTCTGCAAGCTCAGGCCATATAGAAGCCACAGTCCAATGTTCATCACACAACTAGACCTGAACCAAAAGTCAGAGTACCAAACAAGACCTTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

731

Amino Acids

79.76

Weight (kDa)

9.37

Isoelectric Point (pI)

76.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MOV-10_beta-barrel PF21634 364 - 440 2.6e-09 Helicase MOV-10, beta-barrel domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000595)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g37100 FvH4_4g37100 FvH4_4g37100
malus_domestica MD13G1238300.v1.1 MD16G1243100.v1.1 MD16G1243200.v1.1
prunus_persica Prupe.1G070100_v2.0.a1 Prupe.1G070200_v2.0.a1 Prupe.1G070300_v2.0.a1 Prupe.1G070300_v2.0.a1
pyrus_communis pycom13g21070 pycom16g20390 pycom16g20400
rosa_chinensis RchiOBHm_Chr1g0318071 RchiOBHm_Chr1g0318111 RchiOBHm_Chr4g0446861 RchiOBHm_Chr4g0446881 RchiOBHm_Chr4g0446891
rosa_laevigata RLG00000005623 RLG00000005624 RLG00000009337 RLG00000030560 RLG00000030566
rosa_multiflora Rmu_co8177450.1_g000001 Rmu_sc0001753.1_g000012 Rmu_sc0001753.1_g000013 Rmu_sc0001753.1_g000014 Rmu_sc0001753.1_g000015 Rmu_sc0011424.1_g000009 Rmu_sc0011424.1_g000020 Rmu_sc0034555.1_g000001 Rmu_ssc0000008.1_g000002
rosa_roxburghii Rroxscaffold_4G00329530 Rroxscaffold_4G00329590 Rroxscaffold_4G00329800 Rroxscaffold_4G00329810 Rroxscaffold_4G00329860 Rroxscaffold_4G00329910 Rroxscaffold_4G00330030 Rroxscaffold_5G00343610 Rroxscaffold_5G00348280 Rroxscaffold_5G00387220 Rroxscaffold_5G00387230
rosa_rugosa Rorug01G0019100 Rorug01G0019100 Rorug01G0019500 Rorug01G0019600 Rorug04G0010100 Rorug04G0375200 Rorug04G0375300 Rorug04G0375400 Rorug04G0375500 Rorug04G0375600 Rorug04G0375700
rosa_samantha Rh1AG030700 Rh1AG030900 Rh1BG026900 Rh1CG029500 Rh1CG029700 Rh1DG040500 Rh4AG088100 Rh4AG436700 Rh4AG436800 Rh4AG436900 Rh4BG084700 Rh4BG445800 Rh4BG445900 Rh4BG446100 Rh4CG096300 Rh4CG463100 Rh4CG463200 Rh4CG463400 Rh4DG444500 Rh4DG444600 Rh4DG444700
rosa_wichuraiana Rw1G002420 Rw1G002440 Rw1G002480 Rw4G037360 Rw4G037370 Rw4G037380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1047, 1074
AarI CACCTGC 3 cut(s) 292, 373, 574
Acc36I ACCTGC 3 cut(s) 292, 373, 574
Acc65I GGTACC 1 cut(s) 1768
AccB1I GGYRCC 1 cut(s) 1768
AccBSI CCGCTC 3 cut(s) 956, 1788, 2038
AccI GTMKAC 2 cut(s) 1617, 1724
AccII CGCG 2 cut(s) 1251, 2049
AccIII TCCGGA 1 cut(s) 1543
AciI CCGC 5 cut(s) 109, 150, 954, 1786, 2036
AclI AACGTT 1 cut(s) 1483
AclWI GGATC 2 cut(s) 833, 846
AcsI RAATTY 1 cut(s) 1262
AcuI CTGAAG 3 cut(s) 15, 89, 990
AfaI GTAC 2 cut(s) 1770, 2174
AfiI CCNNNNNNNGG 3 cut(s) 1009, 1060, 1217
AflII CTTAAG 1 cut(s) 1504
AgsI TTSAA 9 cut(s) 59, 833, 1009, 1041, 1262, 1327, 1342, 1587, 2093
AjuI GAANNNNNNNTTGG 2 cut(s) 1733, 1765
AloI GAACNNNNNNTCC 2 cut(s) 1137, 1169
Alw21I GWGCWC 1 cut(s) 1666
Alw26I GTCTC 1 cut(s) 61
Alw44I GTGCAC 1 cut(s) 1662
AlwI GGATC 2 cut(s) 833, 846
AlwNI CAGNNNCTG 1 cut(s) 1680
Aor13HI TCCGGA 1 cut(s) 1543
AoxI GGCC 3 cut(s) 1219, 1995, 2111
ApaLI GTGCAC 1 cut(s) 1662
ApeKI GCWGC 7 cut(s) 112, 527, 1367, 1677, 1680, 1745, 1904
ApoI RAATTY 1 cut(s) 1262
ArsI GACNNNNNNTTYG 2 cut(s) 1094, 1126
Asp718I GGTACC 1 cut(s) 1768
AspLEI GCGC 2 cut(s) 901, 2051
AspS9I GGNCC 3 cut(s) 1174, 1219, 1541
AsuC2I CCSGG 1 cut(s) 1841
AsuHPI GGTGA 5 cut(s) 1512, 1629, 1717, 1723, 1864
AvaII GGWCC 2 cut(s) 1174, 1541
AxyI CCTNAGG 3 cut(s) 864, 1140, 1773
BaeGI GKGCMC 2 cut(s) 728, 1666
BaeI ACNNNNGTAYC 2 cut(s) 1628, 1661
BamHI GGATCC 1 cut(s) 838
BanI GGYRCC 1 cut(s) 1768
BanII GRGCYC 3 cut(s) 1212, 1351, 1520
BauI CACGAG 1 cut(s) 1416
BbsI GAAGAC 2 cut(s) 1098, 1107
Bbv12I GWGCWC 1 cut(s) 1666
BbvI GCAGC 7 cut(s) 124, 539, 1354, 1664, 1667, 1732, 1891
BccI CCATC 8 cut(s) 85, 187, 193, 199, 649, 661, 700, 706
BclI TGATCA 2 cut(s) 882, 1471
BcnI CCSGG 1 cut(s) 1841
BcoDI GTCTC 1 cut(s) 61
BfaI CTAG 2 cut(s) 500, 2150
BfmI CTRYAG 2 cut(s) 525, 1488
BfrI CTTAAG 1 cut(s) 1504
BfuAI ACCTGC 3 cut(s) 292, 373, 574
BisI GCNGC 9 cut(s) 110, 113, 528, 954, 1368, 1678, 1681, 1746, 1905
BlsI GCNGC 9 cut(s) 111, 114, 529, 955, 1369, 1679, 1682, 1747, 1906
Bme1390I CCNGG 1 cut(s) 1841
Bme18I GGWCC 2 cut(s) 1174, 1541
BmgT120I GGNCC 3 cut(s) 1174, 1219, 1541
BmiI GGNNCC 2 cut(s) 840, 1770
BmrFI CCNGG 1 cut(s) 1841
BmsI GCATC 6 cut(s) 296, 377, 578, 1366, 1379, 1927
BpiI GAAGAC 2 cut(s) 1098, 1107
BpmI CTGGAG 5 cut(s) 222, 303, 588, 657, 1614
Bpu10I CCTNAGC 1 cut(s) 2108
BpuEI CTTGAG 1 cut(s) 2060
BpuMI CCSGG 1 cut(s) 1841
BsaBI GATNNNNATC 1 cut(s) 1476
BsaI GGTCTC 1 cut(s) 61
BsaWI WCCGGW 2 cut(s) 1053, 1543
BsaXI ACNNNNNCTCC 2 cut(s) 1587, 1617
Bsc4I CCNNNNNNNGG 3 cut(s) 1009, 1060, 1217
Bse1I ACTGG 5 cut(s) 605, 1187, 1543, 1597, 2020
Bse21I CCTNAGG 3 cut(s) 864, 1140, 1773
Bse3DI GCAATG 1 cut(s) 2055
Bse8I GATNNNNATC 1 cut(s) 1476
BseAI TCCGGA 1 cut(s) 1543
BseJI GATNNNNATC 1 cut(s) 1476
BseLI CCNNNNNNNGG 3 cut(s) 1009, 1060, 1217
BseMI GCAATG 1 cut(s) 2055
BseMII CTCAG 5 cut(s) 855, 1131, 1197, 1764, 2122
BseNI ACTGG 5 cut(s) 605, 1187, 1543, 1597, 2020
BseRI GAGGAG 1 cut(s) 751
BseSI GKGCMC 2 cut(s) 728, 1666
BseXI GCAGC 7 cut(s) 124, 539, 1354, 1664, 1667, 1732, 1891
BseYI CCCAGC 9 cut(s) 265, 346, 400, 427, 454, 544, 574, 622, 754
Bsh1236I CGCG 2 cut(s) 1251, 2049
BshFI GGCC 3 cut(s) 1221, 1997, 2113
BshNI GGYRCC 1 cut(s) 1768
BsiHKAI GWGCWC 1 cut(s) 1666
BsiSI CCGG 4 cut(s) 1054, 1544, 1840, 1958
BslFI GGGAC 2 cut(s) 1181, 2085
BslI CCNNNNNNNGG 3 cut(s) 1009, 1060, 1217
BsmAI GTCTC 1 cut(s) 61
BsmFI GGGAC 2 cut(s) 1181, 2085
BsmI GAATGC 2 cut(s) 1650, 1748
BsnI GGCC 3 cut(s) 1221, 1997, 2113
Bso31I GGTCTC 1 cut(s) 61
Bsp1286I GDGCHC 5 cut(s) 728, 1212, 1351, 1520, 1666
Bsp13I TCCGGA 1 cut(s) 1543
Bsp143I GATC 5 cut(s) 838, 882, 1129, 1471, 1477
BspACI CCGC 5 cut(s) 109, 150, 954, 1786, 2036
BspANI GGCC 3 cut(s) 1221, 1997, 2113
BspCNI CTCAG 5 cut(s) 856, 1132, 1198, 1765, 2121
BspEI TCCGGA 1 cut(s) 1543
BspFNI CGCG 2 cut(s) 1251, 2049
BspHI TCATGA 1 cut(s) 855
BspLI GGNNCC 2 cut(s) 840, 1770
BspMAI CTGCAG 2 cut(s) 529, 1492
BspMI ACCTGC 3 cut(s) 292, 373, 574
BspPI GGATC 2 cut(s) 833, 846
BspT107I GGYRCC 1 cut(s) 1768
BspTI CTTAAG 1 cut(s) 1504
BspTNI GGTCTC 1 cut(s) 61
BsrBI CCGCTC 3 cut(s) 956, 1788, 2038
BsrDI GCAATG 1 cut(s) 2055
BsrI ACTGG 5 cut(s) 605, 1187, 1543, 1597, 2020
BssMI GATC 5 cut(s) 838, 882, 1129, 1471, 1477
BssNAI GTATAC 1 cut(s) 1618
BssSI CACGAG 1 cut(s) 1416
Bst1107I GTATAC 1 cut(s) 1618
Bst2BI CACGAG 1 cut(s) 1416
Bst4CI ACNGT 4 cut(s) 1409, 1550, 2005, 2129
Bst6I CTCTTC 5 cut(s) 769, 803, 828, 1190, 1403
BstAFI CTTAAG 1 cut(s) 1504
BstAPI GCANNNNNTGC 1 cut(s) 1376
BstC8I GCNNGC 2 cut(s) 1423, 2105
BstDEI CTNAG 5 cut(s) 864, 1140, 1206, 1773, 2108
BstENI CCTNNNNNAGG 1 cut(s) 1007
BstFNI CGCG 2 cut(s) 1251, 2049
BstHHI GCGC 2 cut(s) 901, 2051
BstKTI GATC 5 cut(s) 841, 885, 1132, 1474, 1480
BstMAI GTCTC 1 cut(s) 61
BstMBI GATC 5 cut(s) 838, 882, 1129, 1471, 1477
BstMWI GCNNNNNNNGC 8 cut(s) 106, 575, 1355, 1364, 1376, 1745, 1994, 2057
BstNSI RCATGY 1 cut(s) 582
BstSCI CCNGG 1 cut(s) 1839
BstSFI CTRYAG 2 cut(s) 525, 1488
BstSLI GKGCMC 2 cut(s) 728, 1666
BstUI CGCG 2 cut(s) 1251, 2049
BstV1I GCAGC 7 cut(s) 124, 539, 1354, 1664, 1667, 1732, 1891
BstV2I GAAGAC 2 cut(s) 1098, 1107
BstX2I RGATCY 1 cut(s) 838
BstXI CCANNNNNNTGG 1 cut(s) 803
BstYI RGATCY 1 cut(s) 838
BstZ17I GTATAC 1 cut(s) 1618
Bsu36I CCTNAGG 3 cut(s) 864, 1140, 1773
BsuRI GGCC 3 cut(s) 1221, 1997, 2113
BtsIMutI CAGTG 1 cut(s) 1194
BveI ACCTGC 3 cut(s) 292, 373, 574
Cac8I GCNNGC 2 cut(s) 1423, 2105
CaiI CAGNNNCTG 1 cut(s) 1680
CciI TCATGA 1 cut(s) 855
CfoI GCGC 2 cut(s) 901, 2051
Cfr13I GGNCC 3 cut(s) 1174, 1219, 1541
Csp6I GTAC 2 cut(s) 1769, 2173
CviAII CATG 5 cut(s) 579, 856, 1355, 1889, 2024
CviQI GTAC 2 cut(s) 1769, 2173
DdeI CTNAG 5 cut(s) 864, 1140, 1206, 1773, 2108
DpnI GATC 5 cut(s) 840, 884, 1131, 1473, 1479
DpnII GATC 5 cut(s) 838, 882, 1129, 1471, 1477
Eam1104I CTCTTC 5 cut(s) 769, 803, 828, 1190, 1403
EarI CTCTTC 5 cut(s) 769, 803, 828, 1190, 1403
Eco24I GRGCYC 3 cut(s) 1212, 1351, 1520
Eco31I GGTCTC 1 cut(s) 61
Eco32I GATATC 1 cut(s) 1312
Eco47I GGWCC 2 cut(s) 1174, 1541
Eco57I CTGAAG 3 cut(s) 15, 89, 990
Eco81I CCTNAGG 3 cut(s) 864, 1140, 1773
EcoNI CCTNNNNNAGG 1 cut(s) 1007
EcoO109I RGGNCCY 1 cut(s) 1219
EcoRV GATATC 1 cut(s) 1312
EcoT38I GRGCYC 3 cut(s) 1212, 1351, 1520
FaeI CATG 5 cut(s) 582, 859, 1358, 1892, 2027
FalI AAGNNNNNCTT 4 cut(s) 932, 964, 1567, 1599
FaqI GGGAC 2 cut(s) 1181, 2085
FatI CATG 5 cut(s) 578, 855, 1354, 1888, 2023
FbaI TGATCA 2 cut(s) 882, 1471
FblI GTMKAC 2 cut(s) 1617, 1724
Fnu4HI GCNGC 9 cut(s) 110, 113, 528, 954, 1368, 1678, 1681, 1746, 1905
FriOI GRGCYC 3 cut(s) 1212, 1351, 1520
Fsp4HI GCNGC 9 cut(s) 110, 113, 528, 954, 1368, 1678, 1681, 1746, 1905
FspBI CTAG 2 cut(s) 500, 2150
GlaI GCGC 2 cut(s) 900, 2050
GluI GCNGC 9 cut(s) 110, 113, 528, 954, 1368, 1678, 1681, 1746, 1905
GsaI CCCAGC 9 cut(s) 269, 350, 404, 431, 458, 548, 578, 626, 758
GsuI CTGGAG 5 cut(s) 222, 303, 588, 657, 1614
HaeIII GGCC 3 cut(s) 1221, 1997, 2113
HapII CCGG 4 cut(s) 1054, 1544, 1840, 1958
HhaI GCGC 2 cut(s) 901, 2051
Hin1II CATG 5 cut(s) 582, 859, 1358, 1892, 2027
Hin6I GCGC 2 cut(s) 899, 2049
HinP1I GCGC 2 cut(s) 899, 2049
HincII GTYRAC 1 cut(s) 1725
HindII GTYRAC 1 cut(s) 1725
HindIII AAGCTT 2 cut(s) 1466, 1699
HinfI GANTC 9 cut(s) 859, 913, 1412, 1510, 1714, 1721, 1805, 2073, 2097
HpaII CCGG 4 cut(s) 1054, 1544, 1840, 1958
HphI GGTGA 5 cut(s) 1512, 1629, 1717, 1723, 1864
Hpy166II GTNNAC 3 cut(s) 1618, 1664, 1725
Hpy8I GTNNAC 3 cut(s) 1618, 1664, 1725
HpyCH4III ACNGT 4 cut(s) 1409, 1550, 2005, 2129
HpyCH4IV ACGT 2 cut(s) 119, 1483
HpyF10VI GCNNNNNNNGC 8 cut(s) 106, 575, 1355, 1364, 1376, 1745, 1994, 2057
HpyF3I CTNAG 5 cut(s) 864, 1140, 1206, 1773, 2108
HpySE526I ACGT 2 cut(s) 119, 1483
Hsp92II CATG 5 cut(s) 582, 859, 1358, 1892, 2027
HspAI GCGC 2 cut(s) 899, 2049
Kpn2I TCCGGA 1 cut(s) 1543
KpnI GGTACC 1 cut(s) 1772
Ksp22I TGATCA 2 cut(s) 882, 1471
Kzo9I GATC 5 cut(s) 838, 882, 1129, 1471, 1477
LmnI GCTCC 1 cut(s) 1242
Lsp1109I GCAGC 7 cut(s) 124, 539, 1354, 1664, 1667, 1732, 1891
LweI GCATC 6 cut(s) 296, 377, 578, 1366, 1379, 1927
MaeI CTAG 2 cut(s) 500, 2150
MaeII ACGT 2 cut(s) 119, 1483
MaeIII GTNAC 5 cut(s) 1015, 1409, 1534, 1635, 2011
MalI GATC 5 cut(s) 840, 884, 1131, 1473, 1479
MbiI CCGCTC 3 cut(s) 956, 1788, 2038
MboI GATC 5 cut(s) 838, 882, 1129, 1471, 1477
MflI RGATCY 1 cut(s) 838
MhlI GDGCHC 5 cut(s) 728, 1212, 1351, 1520, 1666
MluCI AATT 6 cut(s) 294, 375, 894, 1041, 1262, 1622
MlyI GAGTC 5 cut(s) 1406, 1730, 1814, 2067, 2091
MmeI TCCRAC 4 cut(s) 1008, 1320, 1474, 1906
MroI TCCGGA 1 cut(s) 1543
MseI TTAA 4 cut(s) 738, 1215, 1505, 1574
MspA1I CMGCKG 2 cut(s) 109, 1677
MspCI CTTAAG 1 cut(s) 1504
MspI CCGG 4 cut(s) 1054, 1544, 1840, 1958
MspR9I CCNGG 1 cut(s) 1841
Mva1269I GAATGC 2 cut(s) 1650, 1748
MvnI CGCG 2 cut(s) 1251, 2049
MwoI GCNNNNNNNGC 8 cut(s) 106, 575, 1355, 1364, 1376, 1745, 1994, 2057
NciI CCSGG 1 cut(s) 1841
NdeII GATC 5 cut(s) 838, 882, 1129, 1471, 1477
NlaIII CATG 5 cut(s) 582, 859, 1358, 1892, 2027
NlaIV GGNNCC 2 cut(s) 840, 1770
NmuCI GTSAC 3 cut(s) 1015, 1409, 1635
NspI RCATGY 1 cut(s) 582
PagI TCATGA 1 cut(s) 855
PaqCI CACCTGC 3 cut(s) 292, 373, 574
PctI GAATGC 2 cut(s) 1650, 1748
PfeI GAWTC 4 cut(s) 859, 913, 1510, 1714
PflFI GACNNNGTC 1 cut(s) 1172
PfoI TCCNGGA 1 cut(s) 1839
PkrI GCNGC 9 cut(s) 111, 114, 529, 955, 1369, 1679, 1682, 1747, 1906
PleI GAGTC 5 cut(s) 1406, 1729, 1813, 2067, 2091
PpsI GAGTC 5 cut(s) 1406, 1729, 1813, 2067, 2091
PsiI TTATAA 2 cut(s) 1047, 1074
Psp1406I AACGTT 1 cut(s) 1483
PspFI CCCAGC 9 cut(s) 265, 346, 400, 427, 454, 544, 574, 622, 754
PspN4I GGNNCC 2 cut(s) 840, 1770
PspPI GGNCC 3 cut(s) 1174, 1219, 1541
PstI CTGCAG 2 cut(s) 529, 1492
PstNI CAGNNNCTG 1 cut(s) 1680
PsuI RGATCY 1 cut(s) 838
PsyI GACNNNGTC 1 cut(s) 1172
PvuII CAGCTG 1 cut(s) 1677
RsaI GTAC 2 cut(s) 1770, 2174
RsaNI GTAC 2 cut(s) 1769, 2173
SalI GTCGAC 1 cut(s) 1723
SaqAI TTAA 4 cut(s) 738, 1215, 1505, 1574
SatI GCNGC 9 cut(s) 110, 113, 528, 954, 1368, 1678, 1681, 1746, 1905
Sau3AI GATC 5 cut(s) 838, 882, 1129, 1471, 1477
Sau96I GGNCC 3 cut(s) 1174, 1219, 1541
SchI GAGTC 5 cut(s) 1406, 1730, 1814, 2067, 2091
ScrFI CCNGG 1 cut(s) 1841
SduI GDGCHC 5 cut(s) 728, 1212, 1351, 1520, 1666
SfaNI GCATC 6 cut(s) 296, 377, 578, 1366, 1379, 1927
SfcI CTRYAG 2 cut(s) 525, 1488
SinI GGWCC 2 cut(s) 1174, 1541
SmlI CTYRAG 2 cut(s) 1504, 2075
SmoI CTYRAG 2 cut(s) 1504, 2075
Sse9I AATT 6 cut(s) 294, 375, 894, 1041, 1262, 1622
SsiI CCGC 5 cut(s) 109, 150, 954, 1786, 2036
SspI AATATT 1 cut(s) 39
SspMI CTAG 2 cut(s) 500, 2150
StyD4I CCNGG 1 cut(s) 1839
TaaI ACNGT 4 cut(s) 1409, 1550, 2005, 2129
TaiI ACGT 2 cut(s) 122, 1486
TaqI TCGA 2 cut(s) 873, 1724
TasI AATT 6 cut(s) 294, 375, 894, 1041, 1262, 1622
TauI GCSGC 2 cut(s) 112, 956
TfiI GAWTC 4 cut(s) 859, 913, 1510, 1714
Tru1I TTAA 4 cut(s) 738, 1215, 1505, 1574
Tru9I TTAA 4 cut(s) 738, 1215, 1505, 1574
TscAI CASTG 1 cut(s) 1194
TseFI GTSAC 3 cut(s) 1015, 1409, 1635
TseI GCWGC 7 cut(s) 112, 527, 1367, 1677, 1680, 1745, 1904
Tsp45I GTSAC 3 cut(s) 1015, 1409, 1635
TspRI CASTG 1 cut(s) 1194
Tth111I GACNNNGTC 1 cut(s) 1172
Vha464I CTTAAG 1 cut(s) 1504
VneI GTGCAC 1 cut(s) 1662
VpaK11BI GGWCC 2 cut(s) 1174, 1541
XagI CCTNNNNNAGG 1 cut(s) 1007
XapI RAATTY 1 cut(s) 1262
XceI RCATGY 1 cut(s) 582
XmiI GTMKAC 2 cut(s) 1617, 1724
XspI CTAG 2 cut(s) 500, 2150
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.