Rw1G002480

RNA helicase SDE3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000595
Physical Location & Seq
Reverse (-)
4500649 .. 4503853
3205 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G002480.1

Sequence Viewer

Length: 2082 bp
ATGATGAGGTTGGGGATAATCATTGGGGCTTCAGCAATATTATTGTGGGAATACTTTGAAAATAAGAGACCCACACCATCATCATCATCCCCTGAAACGCCACCTCAGCGGCAGCCAACGTCATCTAATTCTAAACCACATCCAGCCTCCGCAGAACCCCCACCATATTATTCTAAACCATCTCCATCTCCATCTCCATCTTCCCCGAAACCCCCACCATATTCTTCTAAACCACCTTCATATCCAGCTTCTTCTAAACCACCTTCATCCCCAGCTTCTTCTCCCCCACCATATTCTTCTAAACCACCTTCATCCCCAGCTTCTTCTAAACCACCTTCATCCCCAGCTTCTTCTCCCCCACCATATTCTTCTAAACCACCTTCATCCCCAGCTTCTTCTAAACCACCTTCATCTGCAGCTTCCCCACAACCCCCAGCATATTCTTCTAAACCACCTGCATCCCCAGCATGTTCTTCTAAACCACCTTCATCTCCAGTTTCCCCACAACCCCCAGCATATTCTTCTAAACCATCTTCATCTCCATCTTCCCCAAAACACCCTCCAGCACATTTTTCTATACCATCTCCATCTCCACCTCCATCTCCCCCAAAGTGCCCTCCTGTCTTTAAGTCAGTTCTACGCCCAGCCTCCTCTTCCTCAAATAATGATGATATAAGTCAAAACCAACAATATGGGAAGAGAACAGCTTATGTGTGTGTTGAAGAGGATCCATTACCTGTATTCATGATTCCTGAGGATGTCGAAGCCTTGATCAAGAAAGAAATTGCGCCCAAAGTTCTGAATCGCCCATTATCTCCCACAACATACAAGGACTACTTTGCCGCTCTCTTATATTCTGAAGATTTCTACTTGGAGAAATGGAGTGATTTCCTTTTGAAGGGTGTGACATTAGCGTTGGATGAAAGTTCAATTTATAAAAACCGGAAAAAGGAAGCACTTTATAAAAATCGGAAAAAGGAAGATAAGGAAGACAAAACCTTTGTAGCATTTGAGCTTGATCGTGTTCCTGAGGAACGACCATTCCTCTTGTCAAGGGACTTGGTCCTTGCACGACCAGTGGGGAAGAGTTCTACTGAGCCCTTTAAGGGCCCCATCCATAAAGTTGTTGGGAGCAATCGCGTTTTGGTTGAATTTGAGGATGGTTTTTATTCTTATCATCATTCCAACAAAAAATATGATATCAGCTTCTCATACAATAGAGTTTGTTTGAAAAGAGCCCACCATGCAGTAAAAGCTGCATCAGATGCCTTGTTTCAGAACTTCCTCTTCCCTGACTGTGACTCACGAGCAAGCATTCCTGCTGCACCAGCTCTGCTTTCTACTACCTATCATAAGCTTGATCAAGAGCAACGTTCTGCAGTCGGACATATCTTAAGGATTCAGGGCTCACCACCTTATGTAGTAACTGGTCCAGACTGTGAAGGCAAATCTTTTGCAAATTTAAGAGAACCTTCAAGAACTGGAGTGGTTGTTAGTGAAGCAGTATACCAATTATGTCGGAAGTCACCAGAGTATCGCATTCTTATCTGTGCGCCTACTAACAGCTGCTGCGATATGCTGATGAGAAGCTTGGTGAAGGTGATTCCAGAGTCTACTATGTTTCGTGCCAATGCTGCATTCCGAGACAGAGATGAGGTACCTGAGGACATCCTCCGTTCATCCCTTTACAAAGAGTCTTGTTTTTCTTTTCCTGCAATAGAGAAACTCCGGGAATACAAGGTGATTTTCTCAACTTTCATGAGTAGCTTCCGACTACATGATAAAGGCATAGTTGCTGGACATTTTAGCCATATTTTTCTGGTGGATGCTTCATCTGCTATTGAGCCGGAAACATTGGTCGCCCTGACTAACTTTGCTGTCAAGGCCACTAGTGTTATAGTTACTGGTAATCATGGAAAGAGTCCGCTCTGGGTTCGCGCTGACATTGCAAGGAAAAAGGGACTCAAGATTTCATACTTTGAACGACTCTGCAAGCTCAGGCCATATAGAAGCCCCAGTCCAATGTTCATCACACAACTAGACCTGAACCAAAAGTCAGAGTACCAAACAAGACCTTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

693

Amino Acids

76.36

Weight (kDa)

9.36

Isoelectric Point (pI)

76.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MOV-10_beta-barrel PF21634 327 - 403 3.8e-09 Helicase MOV-10, beta-barrel domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000595)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g37100 FvH4_4g37100 FvH4_4g37100
malus_domestica MD13G1238300.v1.1 MD16G1243100.v1.1 MD16G1243200.v1.1
prunus_persica Prupe.1G070100_v2.0.a1 Prupe.1G070200_v2.0.a1 Prupe.1G070300_v2.0.a1 Prupe.1G070300_v2.0.a1
pyrus_communis pycom13g21070 pycom16g20390 pycom16g20400
rosa_chinensis RchiOBHm_Chr1g0318071 RchiOBHm_Chr1g0318111 RchiOBHm_Chr4g0446861 RchiOBHm_Chr4g0446881 RchiOBHm_Chr4g0446891
rosa_laevigata RLG00000005623 RLG00000005624 RLG00000009337 RLG00000030560 RLG00000030566
rosa_multiflora Rmu_co8177450.1_g000001 Rmu_sc0001753.1_g000012 Rmu_sc0001753.1_g000013 Rmu_sc0001753.1_g000014 Rmu_sc0001753.1_g000015 Rmu_sc0011424.1_g000009 Rmu_sc0011424.1_g000020 Rmu_sc0034555.1_g000001 Rmu_ssc0000008.1_g000002
rosa_roxburghii Rroxscaffold_4G00329530 Rroxscaffold_4G00329590 Rroxscaffold_4G00329800 Rroxscaffold_4G00329810 Rroxscaffold_4G00329860 Rroxscaffold_4G00329910 Rroxscaffold_4G00330030 Rroxscaffold_5G00343610 Rroxscaffold_5G00348280 Rroxscaffold_5G00387220 Rroxscaffold_5G00387230
rosa_rugosa Rorug01G0019100 Rorug01G0019100 Rorug01G0019500 Rorug01G0019600 Rorug04G0010100 Rorug04G0375200 Rorug04G0375300 Rorug04G0375400 Rorug04G0375500 Rorug04G0375600 Rorug04G0375700
rosa_samantha Rh1AG030700 Rh1AG030900 Rh1BG026900 Rh1CG029500 Rh1CG029700 Rh1DG040500 Rh4AG088100 Rh4AG436700 Rh4AG436800 Rh4AG436900 Rh4BG084700 Rh4BG445800 Rh4BG445900 Rh4BG446100 Rh4CG096300 Rh4CG463100 Rh4CG463200 Rh4CG463400 Rh4DG444500 Rh4DG444600 Rh4DG444700
rosa_wichuraiana Rw1G002420 Rw1G002440 Rw1G002480 Rw4G037360 Rw4G037370 Rw4G037380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 936, 963
AarI CACCTGC 1 cut(s) 463
Acc36I ACCTGC 1 cut(s) 463
Acc65I GGTACC 1 cut(s) 1657
AccB1I GGYRCC 1 cut(s) 1657
AccBSI CCGCTC 2 cut(s) 845, 1927
AccI GTMKAC 2 cut(s) 1506, 1613
AccII CGCG 2 cut(s) 1140, 1938
AciI CCGC 4 cut(s) 109, 150, 843, 1925
AclI AACGTT 1 cut(s) 1372
AclWI GGATC 2 cut(s) 722, 735
AcsI RAATTY 2 cut(s) 1151, 1459
AcuI CTGAAG 2 cut(s) 15, 879
AfaI GTAC 2 cut(s) 1659, 2063
AfiI CCNNNNNNNGG 3 cut(s) 898, 949, 1106
AflII CTTAAG 1 cut(s) 1393
AgsI TTSAA 8 cut(s) 59, 722, 898, 930, 1151, 1231, 1476, 1982
AhlI ACTAGT 1 cut(s) 1889
AjuI GAANNNNNNNTTGG 2 cut(s) 1622, 1654
AloI GAACNNNNNNTCC 2 cut(s) 1026, 1058
Alw26I GTCTC 2 cut(s) 61, 1638
AlwI GGATC 2 cut(s) 722, 735
AlwNI CAGNNNCTG 1 cut(s) 1569
AoxI GGCC 3 cut(s) 1108, 1884, 2000
ApaI GGGCCC 1 cut(s) 1112
ApeKI GCWGC 7 cut(s) 112, 416, 1256, 1322, 1566, 1569, 1634
ApoI RAATTY 2 cut(s) 1151, 1459
ArsI GACNNNNNNTTYG 2 cut(s) 983, 1015
Asp718I GGTACC 1 cut(s) 1657
AspLEI GCGC 3 cut(s) 790, 1555, 1940
AspS9I GGNCC 4 cut(s) 1063, 1108, 1109, 1430
AsuC2I CCSGG 1 cut(s) 1730
AsuHPI GGTGA 5 cut(s) 1401, 1518, 1606, 1612, 1753
AvaII GGWCC 2 cut(s) 1063, 1430
AxyI CCTNAGG 3 cut(s) 753, 1029, 1662
BaeGI GKGCMC 2 cut(s) 617, 1112
BaeI ACNNNNGTAYC 2 cut(s) 1517, 1550
BamHI GGATCC 1 cut(s) 727
BanI GGYRCC 1 cut(s) 1657
BanII GRGCYC 4 cut(s) 1101, 1112, 1240, 1409
BauI CACGAG 1 cut(s) 1305
BbsI GAAGAC 1 cut(s) 996
BbvCI CCTCAGC 1 cut(s) 105
BbvI GCAGC 7 cut(s) 124, 428, 1243, 1309, 1553, 1556, 1621
BclI TGATCA 2 cut(s) 771, 1360
BcnI CCSGG 1 cut(s) 1730
BcoDI GTCTC 2 cut(s) 61, 1638
BcuI ACTAGT 1 cut(s) 1889
BfaI CTAG 2 cut(s) 1890, 2039
BfmI CTRYAG 2 cut(s) 414, 1377
BfrI CTTAAG 1 cut(s) 1393
BfuAI ACCTGC 1 cut(s) 463
BisI GCNGC 9 cut(s) 110, 113, 417, 843, 1257, 1323, 1567, 1570, 1635
BlsI GCNGC 9 cut(s) 111, 114, 418, 844, 1258, 1324, 1568, 1571, 1636
Bme1390I CCNGG 1 cut(s) 1730
Bme18I GGWCC 2 cut(s) 1063, 1430
BmgT120I GGNCC 4 cut(s) 1063, 1108, 1109, 1430
BmiI GGNNCC 4 cut(s) 729, 1110, 1111, 1659
BmrFI CCNGG 1 cut(s) 1730
BmrI ACTGGG 1 cut(s) 2010
BmsI GCATC 4 cut(s) 467, 1255, 1268, 1816
BmuI ACTGGG 1 cut(s) 2010
BpiI GAAGAC 1 cut(s) 996
BpmI CTGGAG 3 cut(s) 477, 546, 1503
Bpu10I CCTNAGC 2 cut(s) 105, 1997
BpuEI CTTGAG 1 cut(s) 1949
BpuMI CCSGG 1 cut(s) 1730
BsaI GGTCTC 1 cut(s) 61
BsaWI WCCGGW 1 cut(s) 942
BsaXI ACNNNNNCTCC 2 cut(s) 1476, 1506
Bsc4I CCNNNNNNNGG 3 cut(s) 898, 949, 1106
Bse1I ACTGG 6 cut(s) 494, 1076, 1432, 1486, 1909, 2016
Bse21I CCTNAGG 3 cut(s) 753, 1029, 1662
Bse3DI GCAATG 1 cut(s) 1944
BseLI CCNNNNNNNGG 3 cut(s) 898, 949, 1106
BseMI GCAATG 1 cut(s) 1944
BseMII CTCAG 6 cut(s) 119, 744, 1020, 1086, 1653, 2011
BseNI ACTGG 6 cut(s) 494, 1076, 1432, 1486, 1909, 2016
BseRI GAGGAG 1 cut(s) 640
BseSI GKGCMC 2 cut(s) 617, 1112
BseXI GCAGC 7 cut(s) 124, 428, 1243, 1309, 1553, 1556, 1621
BseYI CCCAGC 8 cut(s) 271, 316, 343, 388, 433, 463, 511, 643
BsgI GTGCAG 1 cut(s) 1308
Bsh1236I CGCG 2 cut(s) 1140, 1938
BshFI GGCC 3 cut(s) 1110, 1886, 2002
BshNI GGYRCC 1 cut(s) 1657
BsiSI CCGG 3 cut(s) 943, 1729, 1847
BslFI GGGAC 2 cut(s) 1070, 1974
BslI CCNNNNNNNGG 3 cut(s) 898, 949, 1106
BsmAI GTCTC 2 cut(s) 61, 1638
BsmFI GGGAC 2 cut(s) 1070, 1974
BsmI GAATGC 3 cut(s) 1314, 1539, 1637
BsnI GGCC 3 cut(s) 1110, 1886, 2002
Bso31I GGTCTC 1 cut(s) 61
Bsp120I GGGCCC 1 cut(s) 1108
Bsp1286I GDGCHC 5 cut(s) 617, 1101, 1112, 1240, 1409
Bsp143I GATC 4 cut(s) 727, 771, 1018, 1360
BspACI CCGC 4 cut(s) 109, 150, 843, 1925
BspANI GGCC 3 cut(s) 1110, 1886, 2002
BspCNI CTCAG 6 cut(s) 118, 745, 1021, 1087, 1654, 2010
BspFNI CGCG 2 cut(s) 1140, 1938
BspHI TCATGA 2 cut(s) 744, 1758
BspLI GGNNCC 4 cut(s) 729, 1110, 1111, 1659
BspMAI CTGCAG 2 cut(s) 418, 1381
BspMI ACCTGC 1 cut(s) 463
BspPI GGATC 2 cut(s) 722, 735
BspT107I GGYRCC 1 cut(s) 1657
BspTI CTTAAG 1 cut(s) 1393
BspTNI GGTCTC 1 cut(s) 61
BsrBI CCGCTC 2 cut(s) 845, 1927
BsrDI GCAATG 1 cut(s) 1944
BsrI ACTGG 6 cut(s) 494, 1076, 1432, 1486, 1909, 2016
BssMI GATC 4 cut(s) 727, 771, 1018, 1360
BssNAI GTATAC 1 cut(s) 1507
BssSI CACGAG 1 cut(s) 1305
Bst1107I GTATAC 1 cut(s) 1507
Bst2BI CACGAG 1 cut(s) 1305
Bst4CI ACNGT 2 cut(s) 1298, 1439
Bst6I CTCTTC 5 cut(s) 658, 692, 717, 1079, 1292
BstAFI CTTAAG 1 cut(s) 1393
BstAPI GCANNNNNTGC 1 cut(s) 1265
BstC8I GCNNGC 2 cut(s) 1312, 1994
BstDEI CTNAG 6 cut(s) 105, 753, 1029, 1095, 1662, 1997
BstENI CCTNNNNNAGG 1 cut(s) 896
BstFNI CGCG 2 cut(s) 1140, 1938
BstHHI GCGC 3 cut(s) 790, 1555, 1940
BstKTI GATC 4 cut(s) 730, 774, 1021, 1363
BstMAI GTCTC 2 cut(s) 61, 1638
BstMBI GATC 4 cut(s) 727, 771, 1018, 1360
BstNSI RCATGY 1 cut(s) 471
BstSCI CCNGG 1 cut(s) 1728
BstSFI CTRYAG 2 cut(s) 414, 1377
BstSLI GKGCMC 2 cut(s) 617, 1112
BstUI CGCG 2 cut(s) 1140, 1938
BstV1I GCAGC 7 cut(s) 124, 428, 1243, 1309, 1553, 1556, 1621
BstV2I GAAGAC 1 cut(s) 996
BstX2I RGATCY 1 cut(s) 727
BstXI CCANNNNNNTGG 1 cut(s) 692
BstYI RGATCY 1 cut(s) 727
BstZ17I GTATAC 1 cut(s) 1507
Bsu36I CCTNAGG 3 cut(s) 753, 1029, 1662
BsuRI GGCC 3 cut(s) 1110, 1886, 2002
BtsIMutI CAGTG 1 cut(s) 1083
BveI ACCTGC 1 cut(s) 463
Cac8I GCNNGC 2 cut(s) 1312, 1994
CaiI CAGNNNCTG 1 cut(s) 1569
CciI TCATGA 2 cut(s) 744, 1758
CfoI GCGC 3 cut(s) 790, 1555, 1940
Cfr13I GGNCC 4 cut(s) 1063, 1108, 1109, 1430
Csp6I GTAC 2 cut(s) 1658, 2062
CviAII CATG 6 cut(s) 468, 745, 1244, 1759, 1778, 1913
CviQI GTAC 2 cut(s) 1658, 2062
DdeI CTNAG 6 cut(s) 105, 753, 1029, 1095, 1662, 1997
DpnI GATC 4 cut(s) 729, 773, 1020, 1362
DpnII GATC 4 cut(s) 727, 771, 1018, 1360
Eam1104I CTCTTC 5 cut(s) 658, 692, 717, 1079, 1292
EarI CTCTTC 5 cut(s) 658, 692, 717, 1079, 1292
Eco24I GRGCYC 4 cut(s) 1101, 1112, 1240, 1409
Eco31I GGTCTC 1 cut(s) 61
Eco32I GATATC 1 cut(s) 1201
Eco47I GGWCC 2 cut(s) 1063, 1430
Eco57I CTGAAG 2 cut(s) 15, 879
Eco81I CCTNAGG 3 cut(s) 753, 1029, 1662
EcoNI CCTNNNNNAGG 1 cut(s) 896
EcoO109I RGGNCCY 2 cut(s) 1108, 1109
EcoRV GATATC 1 cut(s) 1201
EcoT38I GRGCYC 4 cut(s) 1101, 1112, 1240, 1409
FaeI CATG 6 cut(s) 471, 748, 1247, 1762, 1781, 1916
FalI AAGNNNNNCTT 4 cut(s) 821, 853, 1456, 1488
FaqI GGGAC 2 cut(s) 1070, 1974
FatI CATG 6 cut(s) 467, 744, 1243, 1758, 1777, 1912
FbaI TGATCA 2 cut(s) 771, 1360
FblI GTMKAC 2 cut(s) 1506, 1613
Fnu4HI GCNGC 9 cut(s) 110, 113, 417, 843, 1257, 1323, 1567, 1570, 1635
FriOI GRGCYC 4 cut(s) 1101, 1112, 1240, 1409
Fsp4HI GCNGC 9 cut(s) 110, 113, 417, 843, 1257, 1323, 1567, 1570, 1635
FspBI CTAG 2 cut(s) 1890, 2039
GlaI GCGC 3 cut(s) 789, 1554, 1939
GluI GCNGC 9 cut(s) 110, 113, 417, 843, 1257, 1323, 1567, 1570, 1635
GsaI CCCAGC 8 cut(s) 275, 320, 347, 392, 437, 467, 515, 647
GsuI CTGGAG 3 cut(s) 477, 546, 1503
HaeIII GGCC 3 cut(s) 1110, 1886, 2002
HapII CCGG 3 cut(s) 943, 1729, 1847
HhaI GCGC 3 cut(s) 790, 1555, 1940
Hin1II CATG 6 cut(s) 471, 748, 1247, 1762, 1781, 1916
Hin6I GCGC 3 cut(s) 788, 1553, 1938
HinP1I GCGC 3 cut(s) 788, 1553, 1938
HindIII AAGCTT 2 cut(s) 1355, 1588
HpaII CCGG 3 cut(s) 943, 1729, 1847
HphI GGTGA 5 cut(s) 1401, 1518, 1606, 1612, 1753
Hpy166II GTNNAC 2 cut(s) 1507, 1614
Hpy8I GTNNAC 2 cut(s) 1507, 1614
HpyCH4III ACNGT 2 cut(s) 1298, 1439
HpyCH4IV ACGT 2 cut(s) 119, 1372
HpyF3I CTNAG 6 cut(s) 105, 753, 1029, 1095, 1662, 1997
HpySE526I ACGT 2 cut(s) 119, 1372
Hsp92II CATG 6 cut(s) 471, 748, 1247, 1762, 1781, 1916
HspAI GCGC 3 cut(s) 788, 1553, 1938
KpnI GGTACC 1 cut(s) 1661
Ksp22I TGATCA 2 cut(s) 771, 1360
Kzo9I GATC 4 cut(s) 727, 771, 1018, 1360
LmnI GCTCC 1 cut(s) 1131
Lsp1109I GCAGC 7 cut(s) 124, 428, 1243, 1309, 1553, 1556, 1621
LweI GCATC 4 cut(s) 467, 1255, 1268, 1816
MaeI CTAG 2 cut(s) 1890, 2039
MaeII ACGT 2 cut(s) 119, 1372
MaeIII GTNAC 5 cut(s) 904, 1298, 1423, 1524, 1900
MalI GATC 4 cut(s) 729, 773, 1020, 1362
MbiI CCGCTC 2 cut(s) 845, 1927
MboI GATC 4 cut(s) 727, 771, 1018, 1360
MflI RGATCY 1 cut(s) 727
MhlI GDGCHC 5 cut(s) 617, 1101, 1112, 1240, 1409
MluCI AATT 6 cut(s) 127, 783, 930, 1151, 1459, 1511
MlyI GAGTC 6 cut(s) 1295, 1619, 1703, 1930, 1956, 1980
MmeI TCCRAC 5 cut(s) 897, 1209, 1363, 1499, 1795
MseI TTAA 4 cut(s) 627, 1104, 1394, 1463
MspA1I CMGCKG 2 cut(s) 109, 1566
MspCI CTTAAG 1 cut(s) 1393
MspI CCGG 3 cut(s) 943, 1729, 1847
MspR9I CCNGG 1 cut(s) 1730
Mva1269I GAATGC 3 cut(s) 1314, 1539, 1637
MvnI CGCG 2 cut(s) 1140, 1938
NciI CCSGG 1 cut(s) 1730
NdeII GATC 4 cut(s) 727, 771, 1018, 1360
NlaIII CATG 6 cut(s) 471, 748, 1247, 1762, 1781, 1916
NlaIV GGNNCC 4 cut(s) 729, 1110, 1111, 1659
NmuCI GTSAC 3 cut(s) 904, 1298, 1524
NspI RCATGY 1 cut(s) 471
PagI TCATGA 2 cut(s) 744, 1758
PaqCI CACCTGC 1 cut(s) 463
PctI GAATGC 3 cut(s) 1314, 1539, 1637
PfeI GAWTC 4 cut(s) 748, 802, 1399, 1603
PflFI GACNNNGTC 1 cut(s) 1061
PfoI TCCNGGA 1 cut(s) 1728
PkrI GCNGC 9 cut(s) 111, 114, 418, 844, 1258, 1324, 1568, 1571, 1636
PleI GAGTC 6 cut(s) 1295, 1618, 1702, 1929, 1956, 1980
PpsI GAGTC 6 cut(s) 1295, 1618, 1702, 1929, 1956, 1980
PsiI TTATAA 2 cut(s) 936, 963
Psp1406I AACGTT 1 cut(s) 1372
PspFI CCCAGC 8 cut(s) 271, 316, 343, 388, 433, 463, 511, 643
PspN4I GGNNCC 4 cut(s) 729, 1110, 1111, 1659
PspOMI GGGCCC 1 cut(s) 1108
PspPI GGNCC 4 cut(s) 1063, 1108, 1109, 1430
PstI CTGCAG 2 cut(s) 418, 1381
PstNI CAGNNNCTG 1 cut(s) 1569
PsuI RGATCY 1 cut(s) 727
PsyI GACNNNGTC 1 cut(s) 1061
PvuII CAGCTG 1 cut(s) 1566
RsaI GTAC 2 cut(s) 1659, 2063
RsaNI GTAC 2 cut(s) 1658, 2062
SaqAI TTAA 4 cut(s) 627, 1104, 1394, 1463
SatI GCNGC 9 cut(s) 110, 113, 417, 843, 1257, 1323, 1567, 1570, 1635
Sau3AI GATC 4 cut(s) 727, 771, 1018, 1360
Sau96I GGNCC 4 cut(s) 1063, 1108, 1109, 1430
SchI GAGTC 6 cut(s) 1295, 1619, 1703, 1930, 1956, 1980
ScrFI CCNGG 1 cut(s) 1730
SduI GDGCHC 5 cut(s) 617, 1101, 1112, 1240, 1409
SfaNI GCATC 4 cut(s) 467, 1255, 1268, 1816
SfcI CTRYAG 2 cut(s) 414, 1377
SinI GGWCC 2 cut(s) 1063, 1430
SmlI CTYRAG 2 cut(s) 1393, 1964
SmoI CTYRAG 2 cut(s) 1393, 1964
SpeI ACTAGT 1 cut(s) 1889
Sse9I AATT 6 cut(s) 127, 783, 930, 1151, 1459, 1511
SsiI CCGC 4 cut(s) 109, 150, 843, 1925
SspI AATATT 1 cut(s) 39
SspMI CTAG 2 cut(s) 1890, 2039
StyD4I CCNGG 1 cut(s) 1728
TaaI ACNGT 2 cut(s) 1298, 1439
TaiI ACGT 2 cut(s) 122, 1375
TaqI TCGA 1 cut(s) 762
TasI AATT 6 cut(s) 127, 783, 930, 1151, 1459, 1511
TauI GCSGC 2 cut(s) 112, 845
TfiI GAWTC 4 cut(s) 748, 802, 1399, 1603
Tru1I TTAA 4 cut(s) 627, 1104, 1394, 1463
Tru9I TTAA 4 cut(s) 627, 1104, 1394, 1463
TscAI CASTG 1 cut(s) 1083
TseFI GTSAC 3 cut(s) 904, 1298, 1524
TseI GCWGC 7 cut(s) 112, 416, 1256, 1322, 1566, 1569, 1634
Tsp45I GTSAC 3 cut(s) 904, 1298, 1524
TspGWI ACGGA 1 cut(s) 1664
TspRI CASTG 1 cut(s) 1083
Tth111I GACNNNGTC 1 cut(s) 1061
Vha464I CTTAAG 1 cut(s) 1393
VpaK11BI GGWCC 2 cut(s) 1063, 1430
XagI CCTNNNNNAGG 1 cut(s) 896
XapI RAATTY 2 cut(s) 1151, 1459
XceI RCATGY 1 cut(s) 471
XcmI CCANNNNNNNNNTGG 1 cut(s) 1124
XmiI GTMKAC 2 cut(s) 1506, 1613
XspI CTAG 2 cut(s) 1890, 2039
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.