Rh4AG436800

RNA helicase SDE3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0000595
Physical Location & Seq
Reverse (-)
72975001 .. 72978910
3910 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG436800.1

Sequence Viewer

Length: 2505 bp
ATGTCAAGTTTCTTTGATCTTTTGAGATGCCTTCTGTGTTGTTCCATTGAGCATGATCATTGTGAAAACCGGTTTGAATTTGATACTGCGGAAGAAGCTGAAGCTTCCTCTAGGGTTAACGGAAGACAGAAAACTGAGTCGGTCACTGGTAAGCTCGATCGGAAAACGTACTGCATCTGCGAGTTGGTAATAAGATCGAAGAAACCTCAACACTCCATCTGCTTCTTATATTTTCTAGTGCTAGATGCATTCCAAGGTATCGGTTGCTGCTGGTCATCGGGAGTTCAGCAATATTATGGTGGACATACAAGAATCGCAAACCTCCCCCCCCCAAGAAACAAATGGTCTCGCTTTATACCATTTTCATCATCCCCTCAGCCTCGTCCTGATGGGGTTTGGCTTAATCGGTATTGGTTGCTGCTAGTCATCGGGAGTTCAGCAATATTATGGTTGACAAACAAGAATCGCAAACCTCGCACCCCCAGAAACGAAAGGTCTCGCTTTATACCATTTTCATCATCCCCTCAGCCTCGTCCTGATGGGGTTTGGCTTAATAGGTATCGGTTGCTGCTGGTCATCGGGAGTTCAGCAATATTATGGTTGACAAACAAGAATCGCAAACCTCCCCCCCCAAGAAACAAATGGTCTCGCTTTATACCATTTTCATCATCCCCTCAGCCTCGTCCTGATGGGGTTTGGCTTAATCGGTATTGGTTGCTGCTAGTCATCGGGAGTTCAGCAATATTATGGTTGACAAACAAGAATCGCAAACCTCGCCCCCCTCCAGAAACGAAAGGGGTCGCTTCCCTGAAAAGCCACCTCAGCGTCAGCCAACATCATCTATATCCAGCCTCACCGAAAACCCCAACATATTTTTCTGGACCATCTCCATCTTCCCCAAAACACCCTCCAACATATTTTTCTACACCATCTCCGTCTCCATCTTCCCTGAAACCCCCAGCATATACTTCTAAACCATCTTCATCCTCAACTCCATCTTCCCCAAAACCCGCCCCCAGCATATATTTTTCTCTATCATCTCCGTCTCCATCTTCCCCAAAGCGCCCTCCTGTCTTTAAGTCAGTTCTATGCCCAGCCTCCTCCTTCTCAAATAATGATGATATAATTCAACAACAACGAGACAGGAAGAAAACAGCTTACGTATGTGTTGAAGAGGATTCATTACCTGTATTCACGATTCCTGAGGATATCAAAACCTTGATCAAGAACGAAACTGTGCCCAAAGTTCTCAACTGCTCTTTGTCTCCCACAACGTACAAGGATTACTTTGCTGCTCTGTTATATTCTGAAGATTTCTTCTTGGAGAAATGGAGTGATTTCCTTTTGAAGGCTGTGACATTGGAGTTGAATGAAGCTGAAATTTATAAAAATCAGAAAAAGCAAGATAAAGCCTTTGTGGCATTTGAGCTTGATTCTGTTCCCCAGAATAGGCCATTCCTCTTATCAAGGGACTTGGTCTTTGCACGACCAGTGGGTAGGAGTACTGCTGAGCCCTTTCAGGGTATCATTAATCGCATTGTAAGGAGCAAAACCGTTTTAGTTGAATTTGAAGATGCCTTTTATACTCATCATCATTCCAACAAAAAGTATGATGTCAGCTTCTCATTCAATAGAGTTTGCTTGAAAAGAGCTCACCAAGCAGTTAAAGATGCATCAGAGGACTTGTTTCAGAATTTCCTCTTCCCTCATTGTGACTCACGAGGTAGCATTCCTACTGCACCAGCTCTGCTTTCTACTACCTGTCATAAGCTCGATCGAGATCAACGTTCTGCAGTTGGACATATCTTAAGGATTCAAGGCTCACCTCCTTATGTAGTAACTGGTCCGGACTCTGTACCTGAAGGCAAATCTTTTGCATATTTTAGAGAACCTTCAAGAACTGGAGTAGTTGTTAGTGAAGCAGTATACCAATTATGTCAGAAGTCGCCAGAGTATCGCATTCTTATCTGTGCGCCCACTAACAGCTGCTGCGATGTGCTGATGAGAAGCTTGGGGAAGGTGATTCCAGAGTCGACTATGTTTCGTGCCAATGCTGCATTCCGTGAGAAAGAAGAGGTACCTGAGGATATCCTCCGCTCATCCCTTTACAAAGAGTCCTGTTTTTCTTGTCCTCCAATAGAGGAACTCCGGAAATACAGGGTGATTTTTTCGACTTTCATGAGTAGCTTTCGACTACATGATAAAGGCATAGCTGTTGGACATTTTAGCCATATTTTTCTGGTGGATGCTTCATCCGCTATTGAACCGGAAACATTGGTCGCCCTGACTAACTTTGCTGACAAGACTACTAGTGTTATAGTTACTGGTAAACCTGGAAACAGTCCGCGCTGGGTTCGCGCTGACATTGCAAGGAAAAAGGGACTCAAGATTTCATACTTTGAAAGACTCTGTAAGCTCTGGCCCTATAGAAGCCTCAGTCCAGTGTTCATCACACAATTGGACCTGAACCAAAACTCTGGCTCAGACTACCAAACAGCTTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

834

Amino Acids

94.45

Weight (kDa)

9.45

Isoelectric Point (pI)

59.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MOV-10_beta-barrel PF21634 465 - 541 1.2e-09 Helicase MOV-10, beta-barrel domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000595)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g37100 FvH4_4g37100 FvH4_4g37100
malus_domestica MD13G1238300.v1.1 MD16G1243100.v1.1 MD16G1243200.v1.1
prunus_persica Prupe.1G070100_v2.0.a1 Prupe.1G070200_v2.0.a1 Prupe.1G070300_v2.0.a1 Prupe.1G070300_v2.0.a1
pyrus_communis pycom13g21070 pycom16g20390 pycom16g20400
rosa_chinensis RchiOBHm_Chr1g0318071 RchiOBHm_Chr1g0318111 RchiOBHm_Chr4g0446861 RchiOBHm_Chr4g0446881 RchiOBHm_Chr4g0446891
rosa_laevigata RLG00000005623 RLG00000005624 RLG00000009337 RLG00000030560 RLG00000030566
rosa_multiflora Rmu_co8177450.1_g000001 Rmu_sc0001753.1_g000012 Rmu_sc0001753.1_g000013 Rmu_sc0001753.1_g000014 Rmu_sc0001753.1_g000015 Rmu_sc0011424.1_g000009 Rmu_sc0011424.1_g000020 Rmu_sc0034555.1_g000001 Rmu_ssc0000008.1_g000002
rosa_roxburghii Rroxscaffold_4G00329530 Rroxscaffold_4G00329590 Rroxscaffold_4G00329800 Rroxscaffold_4G00329810 Rroxscaffold_4G00329860 Rroxscaffold_4G00329910 Rroxscaffold_4G00330030 Rroxscaffold_5G00343610 Rroxscaffold_5G00348280 Rroxscaffold_5G00387220 Rroxscaffold_5G00387230
rosa_rugosa Rorug01G0019100 Rorug01G0019100 Rorug01G0019500 Rorug01G0019600 Rorug04G0010100 Rorug04G0375200 Rorug04G0375300 Rorug04G0375400 Rorug04G0375500 Rorug04G0375600 Rorug04G0375700
rosa_samantha Rh1AG030700 Rh1AG030900 Rh1BG026900 Rh1CG029500 Rh1CG029700 Rh1DG040500 Rh4AG088100 Rh4AG436700 Rh4AG436800 Rh4AG436900 Rh4BG084700 Rh4BG445800 Rh4BG445900 Rh4BG446100 Rh4CG096300 Rh4CG463100 Rh4CG463200 Rh4CG463400 Rh4DG444500 Rh4DG444600 Rh4DG444700
rosa_wichuraiana Rw1G002420 Rw1G002440 Rw1G002480 Rw4G037360 Rw4G037370 Rw4G037380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1386
Acc65I GGTACC 1 cut(s) 2077
AccB1I GGYRCC 1 cut(s) 2077
AccBSI CCGCTC 1 cut(s) 2097
AccI GTMKAC 2 cut(s) 1926, 2033
AccII CGCG 2 cut(s) 2347, 2358
AccIII TCCGGA 2 cut(s) 1846, 2148
AciI CCGC 5 cut(s) 89, 1011, 2095, 2256, 2345
AclI AACGTT 1 cut(s) 1786
AcsI RAATTY 4 cut(s) 77, 1380, 1565, 1693
AcuI CTGAAG 3 cut(s) 120, 1329, 1881
AfaI GTAC 5 cut(s) 170, 1277, 1504, 1857, 2079
AfiI CCNNNNNNNGG 3 cut(s) 1348, 1449, 1520
AflII CTTAAG 1 cut(s) 1807
AgeI ACCGGT 1 cut(s) 69
AhlI ACTAGT 1 cut(s) 2309
AjnI CCWGG 1 cut(s) 2332
AjuI GAANNNNNNNTTGG 6 cut(s) 859, 891, 904, 936, 2042, 2074
Alw21I GWGCWC 1 cut(s) 1654
Alw26I GTCTC 7 cut(s) 351, 501, 651, 942, 1050, 1134, 1269
AlwNI CAGNNNCTG 1 cut(s) 1989
Aor13HI TCCGGA 2 cut(s) 1846, 2148
AoxI GGCC 2 cut(s) 1451, 2420
ApeKI GCWGC 8 cut(s) 267, 418, 568, 718, 1292, 1986, 1989, 2054
ApoI RAATTY 4 cut(s) 77, 1380, 1565, 1693
ArsI GACNNNNNNTTYG 2 cut(s) 1463, 1495
AseI ATTAAT 1 cut(s) 1530
AsiGI ACCGGT 1 cut(s) 69
Asp718I GGTACC 1 cut(s) 2077
AspLEI GCGC 4 cut(s) 1065, 1975, 2349, 2360
AspS9I GGNCC 4 cut(s) 881, 1844, 2421, 2461
AsuHPI GGTGA 5 cut(s) 846, 1646, 1815, 2032, 2173
AvaII GGWCC 3 cut(s) 881, 1844, 2461
AxyI CCTNAGG 2 cut(s) 1203, 2082
BaeGI GKGCMC 1 cut(s) 1242
BanI GGYRCC 1 cut(s) 2077
BanII GRGCYC 2 cut(s) 1515, 1654
BauI CACGAG 1 cut(s) 1719
BbsI GAAGAC 1 cut(s) 130
Bbv12I GWGCWC 1 cut(s) 1654
BbvCI CCTCAGC 4 cut(s) 375, 525, 675, 821
BbvI GCAGC 8 cut(s) 254, 405, 555, 705, 1279, 1973, 1976, 2041
BciT130I CCWGG 1 cut(s) 2334
BclI TGATCA 2 cut(s) 55, 1221
BcoDI GTCTC 7 cut(s) 351, 501, 651, 942, 1050, 1134, 1269
BcuI ACTAGT 1 cut(s) 2309
BfaI CTAG 6 cut(s) 111, 236, 242, 422, 722, 2310
BfmI CTRYAG 2 cut(s) 1791, 2425
BfoI RGCGCY 1 cut(s) 1066
BfrI CTTAAG 1 cut(s) 1807
BglI GCCNNNNNGGC 1 cut(s) 1418
BisI GCNGC 8 cut(s) 268, 419, 569, 719, 1293, 1987, 1990, 2055
BlpI GCTNAGC 1 cut(s) 1509
BlsI GCNGC 8 cut(s) 269, 420, 570, 720, 1294, 1988, 1991, 2056
BmcAI AGTACT 1 cut(s) 1504
Bme1390I CCNGG 1 cut(s) 2334
Bme18I GGWCC 3 cut(s) 881, 1844, 2461
BmgT120I GGNCC 4 cut(s) 881, 1844, 2421, 2461
BmiI GGNNCC 1 cut(s) 2079
BmrFI CCNGG 1 cut(s) 2334
BmsI GCATC 7 cut(s) 17, 183, 235, 1564, 1660, 1682, 2236
BpiI GAAGAC 1 cut(s) 130
BpmI CTGGAG 2 cut(s) 768, 1923
Bpu10I CCTNAGC 4 cut(s) 375, 525, 675, 821
Bpu1102I GCTNAGC 1 cut(s) 1509
BpuEI CTTGAG 1 cut(s) 2369
BsaAI YACGTR 1 cut(s) 1162
BsaBI GATNNNNATC 1 cut(s) 1779
BsaI GGTCTC 3 cut(s) 351, 501, 651
BsaJI CCNNGG 1 cut(s) 253
BsaWI WCCGGW 4 cut(s) 69, 1846, 2148, 2266
BsaXI ACNNNNNCTCC 4 cut(s) 916, 946, 1896, 1926
Bsc4I CCNNNNNNNGG 3 cut(s) 1348, 1449, 1520
Bse118I RCCGGY 1 cut(s) 69
Bse1I ACTGG 6 cut(s) 151, 1490, 1846, 1906, 2329, 2441
Bse21I CCTNAGG 2 cut(s) 1203, 2082
Bse3DI GCAATG 1 cut(s) 2364
Bse8I GATNNNNATC 1 cut(s) 1779
BseAI TCCGGA 2 cut(s) 1846, 2148
BseBI CCWGG 1 cut(s) 2334
BseDI CCNNGG 1 cut(s) 253
BseGI GGATG 7 cut(s) 368, 518, 668, 983, 2099, 2251, 2252
BseJI GATNNNNATC 1 cut(s) 1779
BseLI CCNNNNNNNGG 3 cut(s) 1348, 1449, 1520
BseMI GCAATG 1 cut(s) 2364
BseNI ACTGG 6 cut(s) 151, 1490, 1846, 1906, 2329, 2441
BseRI GAGGAG 1 cut(s) 1090
BseSI GKGCMC 1 cut(s) 1242
BseXI GCAGC 8 cut(s) 254, 405, 555, 705, 1279, 1973, 1976, 2041
BseYI CCCAGC 4 cut(s) 958, 1016, 1093, 2349
BsgI GTGCAG 1 cut(s) 1722
Bsh1236I CGCG 2 cut(s) 2347, 2358
Bsh1285I CGRYCG 2 cut(s) 160, 1777
BshFI GGCC 2 cut(s) 1453, 2422
BshNI GGYRCC 1 cut(s) 2077
BshTI ACCGGT 1 cut(s) 69
BsiEI CGRYCG 2 cut(s) 160, 1777
BsiHKAI GWGCWC 1 cut(s) 1654
BsiSI CCGG 4 cut(s) 70, 1847, 2149, 2267
BslFI GGGAC 2 cut(s) 1484, 2394
BslI CCNNNNNNNGG 3 cut(s) 1348, 1449, 1520
BsmAI GTCTC 7 cut(s) 351, 501, 651, 942, 1050, 1134, 1269
BsmBI CGTCTC 2 cut(s) 942, 1050
BsmFI GGGAC 2 cut(s) 1484, 2394
BsmI GAATGC 4 cut(s) 248, 1728, 1959, 2057
BsnI GGCC 2 cut(s) 1453, 2422
Bso31I GGTCTC 3 cut(s) 351, 501, 651
Bsp1286I GDGCHC 3 cut(s) 1242, 1515, 1654
Bsp13I TCCGGA 2 cut(s) 1846, 2148
Bsp143I GATC 7 cut(s) 16, 55, 157, 194, 1221, 1774, 1780
Bsp1720I GCTNAGC 1 cut(s) 1509
BspACI CCGC 5 cut(s) 89, 1011, 2095, 2256, 2345
BspANI GGCC 2 cut(s) 1453, 2422
BspEI TCCGGA 2 cut(s) 1846, 2148
BspFNI CGCG 2 cut(s) 2347, 2358
BspHI TCATGA 1 cut(s) 2178
BspLI GGNNCC 1 cut(s) 2079
BspMAI CTGCAG 1 cut(s) 1795
BspT107I GGYRCC 1 cut(s) 2077
BspTI CTTAAG 1 cut(s) 1807
BspTNI GGTCTC 3 cut(s) 351, 501, 651
BsrBI CCGCTC 1 cut(s) 2097
BsrDI GCAATG 1 cut(s) 2364
BsrFI RCCGGY 1 cut(s) 69
BsrI ACTGG 6 cut(s) 151, 1490, 1846, 1906, 2329, 2441
BssAI RCCGGY 1 cut(s) 69
BssECI CCNNGG 1 cut(s) 253
BssMI GATC 7 cut(s) 16, 55, 157, 194, 1221, 1774, 1780
BssNAI GTATAC 1 cut(s) 1927
BssSI CACGAG 1 cut(s) 1719
BssT1I CCWWGG 1 cut(s) 253
Bst1107I GTATAC 1 cut(s) 1927
Bst2BI CACGAG 1 cut(s) 1719
Bst2UI CCWGG 1 cut(s) 2334
Bst4CI ACNGT 3 cut(s) 1237, 1555, 2342
Bst6I CTCTTC 3 cut(s) 1167, 1706, 2067
BstAFI CTTAAG 1 cut(s) 1807
BstBAI YACGTR 1 cut(s) 1162
BstENI CCTNNNNNAGG 1 cut(s) 1346
BstF5I GGATG 7 cut(s) 368, 518, 668, 983, 2099, 2251, 2252
BstFNI CGCG 2 cut(s) 2347, 2358
BstH2I RGCGCY 1 cut(s) 1066
BstHHI GCGC 4 cut(s) 1065, 1975, 2349, 2360
BstKTI GATC 7 cut(s) 19, 58, 160, 197, 1224, 1777, 1783
BstMAI GTCTC 7 cut(s) 351, 501, 651, 942, 1050, 1134, 1269
BstMBI GATC 7 cut(s) 16, 55, 157, 194, 1221, 1774, 1780
BstMCI CGRYCG 2 cut(s) 160, 1777
BstNI CCWGG 1 cut(s) 2334
BstSCI CCNGG 1 cut(s) 2332
BstSFI CTRYAG 2 cut(s) 1791, 2425
BstSLI GKGCMC 1 cut(s) 1242
BstSNI TACGTA 1 cut(s) 1162
BstUI CGCG 2 cut(s) 2347, 2358
BstV1I GCAGC 8 cut(s) 254, 405, 555, 705, 1279, 1973, 1976, 2041
BstV2I GAAGAC 1 cut(s) 130
BstXI CCANNNNNNTGG 1 cut(s) 2477
BstZ17I GTATAC 1 cut(s) 1927
Bsu36I CCTNAGG 2 cut(s) 1203, 2082
BsuRI GGCC 2 cut(s) 1453, 2422
BtgZI GCGATG 1 cut(s) 2007
BtsCI GGATG 7 cut(s) 368, 518, 668, 983, 2099, 2251, 2252
BtsIMutI CAGTG 3 cut(s) 144, 1497, 2448
CaiI CAGNNNCTG 1 cut(s) 1989
CciI TCATGA 1 cut(s) 2178
CfoI GCGC 4 cut(s) 1065, 1975, 2349, 2360
Cfr10I RCCGGY 1 cut(s) 69
Cfr13I GGNCC 4 cut(s) 881, 1844, 2421, 2461
CseI GACGC 1 cut(s) 814
Csp6I GTAC 5 cut(s) 169, 1276, 1503, 1856, 2078
CspAI ACCGGT 1 cut(s) 69
CviAII CATG 3 cut(s) 53, 2179, 2198
CviQI GTAC 5 cut(s) 169, 1276, 1503, 1856, 2078
DpnI GATC 7 cut(s) 18, 57, 159, 196, 1223, 1776, 1782
DpnII GATC 7 cut(s) 16, 55, 157, 194, 1221, 1774, 1780
Eam1104I CTCTTC 3 cut(s) 1167, 1706, 2067
EarI CTCTTC 3 cut(s) 1167, 1706, 2067
Ecl136II GAGCTC 1 cut(s) 1652
Eco105I TACGTA 1 cut(s) 1162
Eco130I CCWWGG 1 cut(s) 253
Eco24I GRGCYC 2 cut(s) 1515, 1654
Eco31I GGTCTC 3 cut(s) 351, 501, 651
Eco32I GATATC 2 cut(s) 1210, 2089
Eco47I GGWCC 3 cut(s) 881, 1844, 2461
Eco53kI GAGCTC 1 cut(s) 1652
Eco57I CTGAAG 3 cut(s) 120, 1329, 1881
Eco81I CCTNAGG 2 cut(s) 1203, 2082
EcoICRI GAGCTC 1 cut(s) 1652
EcoNI CCTNNNNNAGG 1 cut(s) 1346
EcoRII CCWGG 1 cut(s) 2332
EcoRV GATATC 2 cut(s) 1210, 2089
EcoT14I CCWWGG 1 cut(s) 253
EcoT22I ATGCAT 2 cut(s) 250, 1675
EcoT38I GRGCYC 2 cut(s) 1515, 1654
ErhI CCWWGG 1 cut(s) 253
Esp3I CGTCTC 2 cut(s) 942, 1050
FaeI CATG 3 cut(s) 56, 2182, 2201
FalI AAGNNNNNCTT 2 cut(s) 1271, 1303
FaqI GGGAC 2 cut(s) 1484, 2394
FatI CATG 3 cut(s) 52, 2178, 2197
FauI CCCGC 1 cut(s) 1018
FbaI TGATCA 2 cut(s) 55, 1221
FblI GTMKAC 2 cut(s) 1926, 2033
Fnu4HI GCNGC 8 cut(s) 268, 419, 569, 719, 1293, 1987, 1990, 2055
FokI GGATG 7 cut(s) 355, 505, 655, 970, 2086, 2239, 2258
FriOI GRGCYC 2 cut(s) 1515, 1654
Fsp4HI GCNGC 8 cut(s) 268, 419, 569, 719, 1293, 1987, 1990, 2055
FspBI CTAG 6 cut(s) 111, 236, 242, 422, 722, 2310
GlaI GCGC 4 cut(s) 1064, 1974, 2348, 2359
GluI GCNGC 8 cut(s) 268, 419, 569, 719, 1293, 1987, 1990, 2055
GsaI CCCAGC 4 cut(s) 962, 1020, 1097, 2353
GsuI CTGGAG 2 cut(s) 768, 1923
HaeII RGCGCY 1 cut(s) 1066
HaeIII GGCC 2 cut(s) 1453, 2422
HapII CCGG 4 cut(s) 70, 1847, 2149, 2267
HgaI GACGC 1 cut(s) 814
HhaI GCGC 4 cut(s) 1065, 1975, 2349, 2360
Hin1II CATG 3 cut(s) 56, 2182, 2201
Hin6I GCGC 4 cut(s) 1063, 1973, 2347, 2358
HinP1I GCGC 4 cut(s) 1063, 1973, 2347, 2358
HincII GTYRAC 5 cut(s) 118, 453, 603, 753, 2034
HindII GTYRAC 5 cut(s) 118, 453, 603, 753, 2034
HindIII AAGCTT 2 cut(s) 102, 2008
HpaI GTTAAC 1 cut(s) 118
HpaII CCGG 4 cut(s) 70, 1847, 2149, 2267
HphI GGTGA 5 cut(s) 846, 1646, 1815, 2032, 2173
Hpy166II GTNNAC 8 cut(s) 118, 302, 453, 603, 753, 1927, 2034, 2330
Hpy188I TCNGA 8 cut(s) 162, 1309, 1395, 1678, 1692, 1941, 2485, 2504
Hpy8I GTNNAC 8 cut(s) 118, 302, 453, 603, 753, 1927, 2034, 2330
HpyAV CCTTC 6 cut(s) 41, 1114, 1342, 1856, 1902, 2011
HpyCH4III ACNGT 3 cut(s) 1237, 1555, 2342
HpyCH4IV ACGT 4 cut(s) 167, 1161, 1274, 1786
HpyCH4V TGCA 9 cut(s) 174, 248, 1484, 1673, 1739, 1793, 1877, 2057, 2369
HpySE526I ACGT 4 cut(s) 167, 1161, 1274, 1786
Hsp92II CATG 3 cut(s) 56, 2182, 2201
HspAI GCGC 4 cut(s) 1063, 1973, 2347, 2358
Kpn2I TCCGGA 2 cut(s) 1846, 2148
KpnI GGTACC 1 cut(s) 2081
Ksp22I TGATCA 2 cut(s) 55, 1221
KspAI GTTAAC 1 cut(s) 118
Kzo9I GATC 7 cut(s) 16, 55, 157, 194, 1221, 1774, 1780
LmnI GCTCC 1 cut(s) 1545
Lsp1109I GCAGC 8 cut(s) 254, 405, 555, 705, 1279, 1973, 1976, 2041
LweI GCATC 7 cut(s) 17, 183, 235, 1564, 1660, 1682, 2236
MaeI CTAG 6 cut(s) 111, 236, 242, 422, 722, 2310
MaeII ACGT 4 cut(s) 167, 1161, 1274, 1786
MaeIII GTNAC 5 cut(s) 142, 1354, 1712, 1837, 2320
MalI GATC 7 cut(s) 18, 57, 159, 196, 1223, 1776, 1782
MbiI CCGCTC 1 cut(s) 2097
MboI GATC 7 cut(s) 16, 55, 157, 194, 1221, 1774, 1780
MfeI CAATTG 1 cut(s) 2456
MhlI GDGCHC 3 cut(s) 1242, 1515, 1654
MluCI AATT 7 cut(s) 77, 1125, 1380, 1565, 1693, 1931, 2456
MlyI GAGTC 7 cut(s) 146, 1709, 1844, 2039, 2123, 2376, 2400
MmeI TCCRAC 4 cut(s) 935, 1623, 1777, 2197
Mph1103I ATGCAT 2 cut(s) 250, 1675
MroI TCCGGA 2 cut(s) 1846, 2148
MseI TTAA 8 cut(s) 117, 402, 552, 702, 1077, 1530, 1665, 1808
MspA1I CMGCKG 1 cut(s) 1986
MspCI CTTAAG 1 cut(s) 1807
MspI CCGG 4 cut(s) 70, 1847, 2149, 2267
MspR9I CCNGG 1 cut(s) 2334
MunI CAATTG 1 cut(s) 2456
Mva1269I GAATGC 4 cut(s) 248, 1728, 1959, 2057
MvaI CCWGG 1 cut(s) 2334
MvnI CGCG 2 cut(s) 2347, 2358
NdeII GATC 7 cut(s) 16, 55, 157, 194, 1221, 1774, 1780
NlaIII CATG 3 cut(s) 56, 2182, 2201
NlaIV GGNNCC 1 cut(s) 2079
NmuCI GTSAC 3 cut(s) 142, 1354, 1712
NsiI ATGCAT 2 cut(s) 250, 1675
PagI TCATGA 1 cut(s) 2178
PcsI WCGNNNNNNNCGW 1 cut(s) 1783
PctI GAATGC 4 cut(s) 248, 1728, 1959, 2057
PfeI GAWTC 9 cut(s) 312, 463, 613, 763, 1178, 1198, 1433, 1813, 2023
PflFI GACNNNGTC 1 cut(s) 1475
PinAI ACCGGT 1 cut(s) 69
PkrI GCNGC 8 cut(s) 269, 420, 570, 720, 1294, 1988, 1991, 2056
Ple19I CGATCG 2 cut(s) 160, 1777
PleI GAGTC 7 cut(s) 145, 1709, 1844, 2038, 2122, 2376, 2400
PpsI GAGTC 7 cut(s) 145, 1709, 1844, 2038, 2122, 2376, 2400
Ppu21I YACGTR 1 cut(s) 1162
PshBI ATTAAT 1 cut(s) 1530
PsiI TTATAA 1 cut(s) 1386
Psp124BI GAGCTC 1 cut(s) 1654
Psp1406I AACGTT 1 cut(s) 1786
Psp6I CCWGG 1 cut(s) 2332
PspFI CCCAGC 4 cut(s) 958, 1016, 1093, 2349
PspGI CCWGG 1 cut(s) 2332
PspN4I GGNNCC 1 cut(s) 2079
PspPI GGNCC 4 cut(s) 881, 1844, 2421, 2461
PstI CTGCAG 1 cut(s) 1795
PstNI CAGNNNCTG 1 cut(s) 1989
PsyI GACNNNGTC 1 cut(s) 1475
PvuI CGATCG 2 cut(s) 160, 1777
PvuII CAGCTG 1 cut(s) 1986
RsaI GTAC 5 cut(s) 170, 1277, 1504, 1857, 2079
RsaNI GTAC 5 cut(s) 169, 1276, 1503, 1856, 2078
SacI GAGCTC 1 cut(s) 1654
SalI GTCGAC 1 cut(s) 2032
SaqAI TTAA 8 cut(s) 117, 402, 552, 702, 1077, 1530, 1665, 1808
SatI GCNGC 8 cut(s) 268, 419, 569, 719, 1293, 1987, 1990, 2055
Sau3AI GATC 7 cut(s) 16, 55, 157, 194, 1221, 1774, 1780
Sau96I GGNCC 4 cut(s) 881, 1844, 2421, 2461
ScaI AGTACT 1 cut(s) 1504
SchI GAGTC 7 cut(s) 146, 1709, 1844, 2039, 2123, 2376, 2400
ScrFI CCNGG 1 cut(s) 2334
SduI GDGCHC 3 cut(s) 1242, 1515, 1654
SfaNI GCATC 7 cut(s) 17, 183, 235, 1564, 1660, 1682, 2236
SfcI CTRYAG 2 cut(s) 1791, 2425
SinI GGWCC 3 cut(s) 881, 1844, 2461
SmlI CTYRAG 2 cut(s) 1807, 2384
SmoI CTYRAG 2 cut(s) 1807, 2384
SnaBI TACGTA 1 cut(s) 1162
SpeI ACTAGT 1 cut(s) 2309
Sse9I AATT 7 cut(s) 77, 1125, 1380, 1565, 1693, 1931, 2456
SsiI CCGC 5 cut(s) 89, 1011, 2095, 2256, 2345
SspI AATATT 4 cut(s) 293, 444, 594, 744
SspMI CTAG 6 cut(s) 111, 236, 242, 422, 722, 2310
SstI GAGCTC 1 cut(s) 1654
StyD4I CCNGG 1 cut(s) 2332
StyI CCWWGG 1 cut(s) 253
TaaI ACNGT 3 cut(s) 1237, 1555, 2342
TaiI ACGT 4 cut(s) 170, 1164, 1277, 1789
TaqI TCGA 7 cut(s) 156, 197, 1773, 1777, 2033, 2171, 2191
TaqII GACCGA 1 cut(s) 130
TasI AATT 7 cut(s) 77, 1125, 1380, 1565, 1693, 1931, 2456
TatI WGTACW 1 cut(s) 1502
TfiI GAWTC 9 cut(s) 312, 463, 613, 763, 1178, 1198, 1433, 1813, 2023
Tru1I TTAA 8 cut(s) 117, 402, 552, 702, 1077, 1530, 1665, 1808
Tru9I TTAA 8 cut(s) 117, 402, 552, 702, 1077, 1530, 1665, 1808
TscAI CASTG 3 cut(s) 151, 1497, 2448
TseFI GTSAC 3 cut(s) 142, 1354, 1712
TseI GCWGC 8 cut(s) 267, 418, 568, 718, 1292, 1986, 1989, 2054
Tsp45I GTSAC 3 cut(s) 142, 1354, 1712
TspGWI ACGGA 4 cut(s) 135, 924, 1032, 2051
TspRI CASTG 3 cut(s) 151, 1497, 2448
Tth111I GACNNNGTC 1 cut(s) 1475
Vha464I CTTAAG 1 cut(s) 1807
VpaK11BI GGWCC 3 cut(s) 881, 1844, 2461
VspI ATTAAT 1 cut(s) 1530
XagI CCTNNNNNAGG 1 cut(s) 1346
XapI RAATTY 4 cut(s) 77, 1380, 1565, 1693
XcmI CCANNNNNNNNNTGG 2 cut(s) 339, 639
XmiI GTMKAC 2 cut(s) 1926, 2033
XspI CTAG 6 cut(s) 111, 236, 242, 422, 722, 2310
ZrmI AGTACT 1 cut(s) 1504
Zsp2I ATGCAT 2 cut(s) 250, 1675
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.