Rw4G037370

RNA helicase SDE3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0000595
Physical Location & Seq
Reverse (-)
64006405 .. 64010315
3911 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G037370.1

Sequence Viewer

Length: 2298 bp
ATGTCAAGTTTCTTTGATCTTTTGAGATGCCTTCTGTGTTGTTCCATTGAGCATGATCATTGTGAAAACCGGTTTGAATTTGATACTGCTGAAGAAGCTGAAGCTTCCCCTAGGAATCGCAAACCTCCCCCCCCAAGAAACAAATGGTCTCGCTTTATACCATTTTCATCATCCCCTCAGCCTCGTCCGGATGGGGTTTGGCTTAATCGGTATTGGTTGCTGCTAGTCATCGGGAGTTCAGCAATATTATGGTGGACATACAAGAATCGCAAACCTCGCACCCCCAGAAACGACAGGTCTCGCTTTATACCATTTTCATCAGCCCCTCAGCCTCGTCCTTATGGGGTTTGGCTTAATAGGTATCGGTTGCTGCTGGTCATCGGGAGTCCAGCAATATTATGGTTGACAAACAAGAATCGCAAACCTCCCCCCCCAAGAAACAAATGGTCTCGCTTTATACCATTTTCATCATCCCCTCAGCCTCGTCCTGATGGGGTTTGGCTTAATCGGTATTGGTTGCTGCTAGTCATCGGGAGTTCAGCAATATTATGGTTGACAAACAAGAATCGCAAACCTCGTCCCCCCCCAGAAACGAAAGGGGTCGCTTCCCTGAAAAGCCACCTCAGCCTCAGCCAACATCATCTATATCCAGCCTCACCGAAAACCCCAACATATTTTTCTAGACCATCTCCATCTTCCCCAAAACACCCTCCAACATATTTTTCTACACCATCTCCGTCTCCATCTTCCCCGAAACCCCCAGCATATACTTCTAAACCCTCAACTCCATCTTCCCCAAAACCCGCCCCCAGCATATATTTTTCTCTATCATCTCCGTCTCCATCTTCCCCAAAGCGCCCTCCTGTCTTTAAGTCAGTTCTATGCCCAGCCTCCTCCTCCTCAAATAATGATGATATAATTCAACAACAACGAGACAGGAAGAAAACAGCTTACGTATGTGTTGAAGAGGATTCATTACCTGTATTCACGATTCCTGAGGATATCAAAACCTTGATCAAGAACGAAACTGTGCCCAAAGTTCTCAACTGCTCTTTGTCTCCCACAACGTACAAGGATTACTTTGCTGCTCTGTTATATTCTGAAGATTTCTTCTTGGAGAAATGGAGTGATTTCCTTTTGGAGGCTGTGACATTGGAGTTGAAGGAAGCTGAAATTTATAAAAATCAGAAAAAGGAAGATAAAGCCTTTGTGGCATTTGAGCTTGATTCTGTTCCCCAGAATAGGCCATTCCTCTTATCAAGGGACTTGGTCTTTGCACGACCAGTGGGTAGGAGTACTGCTAAGCCCTTTCAGGGTATCATTAATCGCATTGTAAGGAGCAAAACCGTTTTAGTTGAATTTGAAGATGCCTTTTATACTCATCATCCTTCCAACAAAAAGTATGATGTCAGCTTCTCATTCAATAGAGTTTGCTTGAAAAGGGCTCACCAAGCAGTTAAAGATGCATCAGAGTCCTTGTTTCAGAATTTCCTCTTCCCTCATTGTGACTCACGAGGTAGCATTCCTACTGCACCAGCTCTGCTTTCTACTACCTGTCATAAGCTCGATCGAGATCAACGTTCTGCAGTTGGACATATCTTAAGGATTCAGGGCTCACCACCTTATGTAGTAACTGGTCCGGACTCTGTACCTGAAGGCAAATCTTTTGCATATTTAAGAGAACCTTCAAGAACTGGAGTGGTTGTTAGTGAAGCAGTATACCAATTATGTCAGAAGTCGCCAGAGTATCGGATTCTTATTTGTGCGCCTACTAACAGCTGCTGTGATGTGCTGATGAGAAGCTTGGTGAAGGTGATTCCAGAGTCGACTATGTTTCGTGCCAATGCTGCATTCCGTGAGAAAGAAGAGGTACCTGAGGATATCCTCCGCTCATCCCTTTACAAAGAGTCTTGTTTTTCTTGTCCTTCAATAGAGAAACTCCGGAAATACAGGGTGATTTTCTCGACTTTCATGAGTAGCTTTCGACTACATGATAAAGGCATAGCTGTTGGACATTTTAGCCATATTTTTCTGGTGGATGCTTCATCCGCTATTGAACCGGAAACATTGGTCGCCCTGACTAACTTTGCTGACAAGACTACTAGTGTTATAGTTACTGGTAAACCTGGAAACAGTCCGCGCAGGGTTCGCTCTGACATTGCAAGGAAAAAGGGACTCAAGATTTCATACTTTGAAAGACTCTGTAAGCTCTGGCCCTATAGAAGCCTCAGTCCAGTGTTCATCACACAATTGGACCTGAACCAAAACTCTGGCTCAGACTACCAAACAGCTTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

765

Amino Acids

86.9

Weight (kDa)

9.63

Isoelectric Point (pI)

62.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MOV-10_beta-barrel PF21634 396 - 472 2.3e-09 Helicase MOV-10, beta-barrel domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000595)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g37100 FvH4_4g37100 FvH4_4g37100
malus_domestica MD13G1238300.v1.1 MD16G1243100.v1.1 MD16G1243200.v1.1
prunus_persica Prupe.1G070100_v2.0.a1 Prupe.1G070200_v2.0.a1 Prupe.1G070300_v2.0.a1 Prupe.1G070300_v2.0.a1
pyrus_communis pycom13g21070 pycom16g20390 pycom16g20400
rosa_chinensis RchiOBHm_Chr1g0318071 RchiOBHm_Chr1g0318111 RchiOBHm_Chr4g0446861 RchiOBHm_Chr4g0446881 RchiOBHm_Chr4g0446891
rosa_laevigata RLG00000005623 RLG00000005624 RLG00000009337 RLG00000030560 RLG00000030566
rosa_multiflora Rmu_co8177450.1_g000001 Rmu_sc0001753.1_g000012 Rmu_sc0001753.1_g000013 Rmu_sc0001753.1_g000014 Rmu_sc0001753.1_g000015 Rmu_sc0011424.1_g000009 Rmu_sc0011424.1_g000020 Rmu_sc0034555.1_g000001 Rmu_ssc0000008.1_g000002
rosa_roxburghii Rroxscaffold_4G00329530 Rroxscaffold_4G00329590 Rroxscaffold_4G00329800 Rroxscaffold_4G00329810 Rroxscaffold_4G00329860 Rroxscaffold_4G00329910 Rroxscaffold_4G00330030 Rroxscaffold_5G00343610 Rroxscaffold_5G00348280 Rroxscaffold_5G00387220 Rroxscaffold_5G00387230
rosa_rugosa Rorug01G0019100 Rorug01G0019100 Rorug01G0019500 Rorug01G0019600 Rorug04G0010100 Rorug04G0375200 Rorug04G0375300 Rorug04G0375400 Rorug04G0375500 Rorug04G0375600 Rorug04G0375700
rosa_samantha Rh1AG030700 Rh1AG030900 Rh1BG026900 Rh1CG029500 Rh1CG029700 Rh1DG040500 Rh4AG088100 Rh4AG436700 Rh4AG436800 Rh4AG436900 Rh4BG084700 Rh4BG445800 Rh4BG445900 Rh4BG446100 Rh4CG096300 Rh4CG463100 Rh4CG463200 Rh4CG463400 Rh4DG444500 Rh4DG444600 Rh4DG444700
rosa_wichuraiana Rw1G002420 Rw1G002440 Rw1G002480 Rw4G037360 Rw4G037370 Rw4G037380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1179
Acc65I GGTACC 1 cut(s) 1870
AccB1I GGYRCC 1 cut(s) 1870
AccBSI CCGCTC 1 cut(s) 1890
AccI GTMKAC 2 cut(s) 1719, 1826
AccII CGCG 1 cut(s) 2140
AccIII TCCGGA 3 cut(s) 187, 1639, 1941
AciI CCGC 4 cut(s) 804, 1888, 2049, 2138
AclI AACGTT 1 cut(s) 1579
AcsI RAATTY 4 cut(s) 77, 1173, 1358, 1486
AcuI CTGAAG 4 cut(s) 111, 120, 1122, 1674
AfaI GTAC 4 cut(s) 1070, 1297, 1650, 1872
AfiI CCNNNNNNNGG 3 cut(s) 1141, 1242, 1313
AflII CTTAAG 1 cut(s) 1600
AgeI ACCGGT 1 cut(s) 69
AhlI ACTAGT 1 cut(s) 2102
AjnI CCWGG 1 cut(s) 2125
AjuI GAANNNNNNNTTGG 6 cut(s) 661, 693, 706, 738, 1835, 1867
Alw26I GTCTC 7 cut(s) 153, 303, 453, 744, 843, 927, 1062
AlwNI CAGNNNCTG 1 cut(s) 1782
Aor13HI TCCGGA 3 cut(s) 187, 1639, 1941
AoxI GGCC 2 cut(s) 1244, 2213
ApeKI GCWGC 6 cut(s) 220, 370, 520, 1085, 1779, 1847
ApoI RAATTY 4 cut(s) 77, 1173, 1358, 1486
ArsI GACNNNNNNTTYG 2 cut(s) 1256, 1288
AseI ATTAAT 1 cut(s) 1323
AsiGI ACCGGT 1 cut(s) 69
Asp718I GGTACC 1 cut(s) 1870
AspA2I CCTAGG 1 cut(s) 110
AspLEI GCGC 3 cut(s) 858, 1768, 2142
AspS9I GGNCC 3 cut(s) 1637, 2214, 2254
AsuHPI GGTGA 6 cut(s) 648, 1439, 1608, 1819, 1825, 1966
AvaII GGWCC 2 cut(s) 1637, 2254
AvrII CCTAGG 1 cut(s) 110
AxyI CCTNAGG 2 cut(s) 996, 1875
BaeGI GKGCMC 1 cut(s) 1035
BanI GGYRCC 1 cut(s) 1870
BanII GRGCYC 2 cut(s) 1447, 1616
BauI CACGAG 1 cut(s) 1512
BbvCI CCTCAGC 5 cut(s) 177, 327, 477, 623, 629
BbvI GCAGC 6 cut(s) 207, 357, 507, 1072, 1766, 1834
BccI CCATC 8 cut(s) 185, 485, 694, 700, 739, 751, 796, 850
BciT130I CCWGG 1 cut(s) 2127
BclI TGATCA 2 cut(s) 55, 1014
BcoDI GTCTC 7 cut(s) 153, 303, 453, 744, 843, 927, 1062
BcuI ACTAGT 1 cut(s) 2102
BfaI CTAG 5 cut(s) 111, 224, 524, 681, 2103
BfmI CTRYAG 2 cut(s) 1584, 2218
BfoI RGCGCY 1 cut(s) 859
BfrI CTTAAG 1 cut(s) 1600
BglI GCCNNNNNGGC 1 cut(s) 1211
BisI GCNGC 6 cut(s) 221, 371, 521, 1086, 1780, 1848
BlnI CCTAGG 1 cut(s) 110
BlpI GCTNAGC 1 cut(s) 1302
BlsI GCNGC 6 cut(s) 222, 372, 522, 1087, 1781, 1849
BmcAI AGTACT 1 cut(s) 1297
Bme1390I CCNGG 1 cut(s) 2127
Bme18I GGWCC 2 cut(s) 1637, 2254
BmgT120I GGNCC 3 cut(s) 1637, 2214, 2254
BmiI GGNNCC 1 cut(s) 1872
BmrFI CCNGG 1 cut(s) 2127
BmsI GCATC 5 cut(s) 17, 1357, 1453, 1475, 2029
BpmI CTGGAG 1 cut(s) 1716
Bpu10I CCTNAGC 5 cut(s) 177, 327, 477, 623, 629
Bpu1102I GCTNAGC 1 cut(s) 1302
BpuEI CTTGAG 1 cut(s) 2162
BsaAI YACGTR 1 cut(s) 955
BsaBI GATNNNNATC 1 cut(s) 1572
BsaI GGTCTC 3 cut(s) 153, 303, 453
BsaJI CCNNGG 1 cut(s) 110
BsaWI WCCGGW 5 cut(s) 69, 187, 1639, 1941, 2059
BsaXI ACNNNNNCTCC 4 cut(s) 718, 748, 1689, 1719
Bsc4I CCNNNNNNNGG 3 cut(s) 1141, 1242, 1313
Bse118I RCCGGY 1 cut(s) 69
Bse1I ACTGG 5 cut(s) 1283, 1639, 1699, 2122, 2234
Bse21I CCTNAGG 2 cut(s) 996, 1875
Bse3DI GCAATG 1 cut(s) 2157
Bse8I GATNNNNATC 1 cut(s) 1572
BseAI TCCGGA 3 cut(s) 187, 1639, 1941
BseBI CCWGG 1 cut(s) 2127
BseDI CCNNGG 1 cut(s) 110
BseGI GGATG 7 cut(s) 170, 196, 470, 1384, 1892, 2044, 2045
BseJI GATNNNNATC 1 cut(s) 1572
BseLI CCNNNNNNNGG 3 cut(s) 1141, 1242, 1313
BseMI GCAATG 1 cut(s) 2157
BseMII CTCAG 9 cut(s) 191, 341, 491, 637, 643, 987, 1866, 2242, 2289
BseNI ACTGG 5 cut(s) 1283, 1639, 1699, 2122, 2234
BseRI GAGGAG 3 cut(s) 883, 886, 889
BseSI GKGCMC 1 cut(s) 1035
BseXI GCAGC 6 cut(s) 207, 357, 507, 1072, 1766, 1834
BseYI CCCAGC 3 cut(s) 760, 809, 886
BsgI GTGCAG 1 cut(s) 1515
Bsh1236I CGCG 1 cut(s) 2140
Bsh1285I CGRYCG 1 cut(s) 1570
BshFI GGCC 2 cut(s) 1246, 2215
BshNI GGYRCC 1 cut(s) 1870
BshTI ACCGGT 1 cut(s) 69
BsiEI CGRYCG 1 cut(s) 1570
BsiSI CCGG 5 cut(s) 70, 188, 1640, 1942, 2060
BslFI GGGAC 3 cut(s) 564, 1277, 2187
BslI CCNNNNNNNGG 3 cut(s) 1141, 1242, 1313
BsmAI GTCTC 7 cut(s) 153, 303, 453, 744, 843, 927, 1062
BsmBI CGTCTC 2 cut(s) 744, 843
BsmFI GGGAC 3 cut(s) 564, 1277, 2187
BsmI GAATGC 2 cut(s) 1521, 1850
BsnI GGCC 2 cut(s) 1246, 2215
Bso31I GGTCTC 3 cut(s) 153, 303, 453
Bsp1286I GDGCHC 3 cut(s) 1035, 1447, 1616
Bsp13I TCCGGA 3 cut(s) 187, 1639, 1941
Bsp143I GATC 5 cut(s) 16, 55, 1014, 1567, 1573
Bsp1720I GCTNAGC 1 cut(s) 1302
BspACI CCGC 4 cut(s) 804, 1888, 2049, 2138
BspANI GGCC 2 cut(s) 1246, 2215
BspCNI CTCAG 9 cut(s) 190, 340, 490, 636, 642, 988, 1867, 2241, 2288
BspEI TCCGGA 3 cut(s) 187, 1639, 1941
BspFNI CGCG 1 cut(s) 2140
BspHI TCATGA 1 cut(s) 1971
BspLI GGNNCC 1 cut(s) 1872
BspMAI CTGCAG 1 cut(s) 1588
BspT107I GGYRCC 1 cut(s) 1870
BspTI CTTAAG 1 cut(s) 1600
BspTNI GGTCTC 3 cut(s) 153, 303, 453
BsrBI CCGCTC 1 cut(s) 1890
BsrDI GCAATG 1 cut(s) 2157
BsrFI RCCGGY 1 cut(s) 69
BsrI ACTGG 5 cut(s) 1283, 1639, 1699, 2122, 2234
BssAI RCCGGY 1 cut(s) 69
BssECI CCNNGG 1 cut(s) 110
BssMI GATC 5 cut(s) 16, 55, 1014, 1567, 1573
BssNAI GTATAC 1 cut(s) 1720
BssSI CACGAG 1 cut(s) 1512
BssT1I CCWWGG 1 cut(s) 110
Bst1107I GTATAC 1 cut(s) 1720
Bst2BI CACGAG 1 cut(s) 1512
Bst2UI CCWGG 1 cut(s) 2127
Bst4CI ACNGT 3 cut(s) 1030, 1348, 2135
Bst6I CTCTTC 3 cut(s) 960, 1499, 1860
BstAFI CTTAAG 1 cut(s) 1600
BstBAI YACGTR 1 cut(s) 955
BstENI CCTNNNNNAGG 1 cut(s) 1139
BstF5I GGATG 7 cut(s) 170, 196, 470, 1384, 1892, 2044, 2045
BstFNI CGCG 1 cut(s) 2140
BstH2I RGCGCY 1 cut(s) 859
BstHHI GCGC 3 cut(s) 858, 1768, 2142
BstKTI GATC 5 cut(s) 19, 58, 1017, 1570, 1576
BstMAI GTCTC 7 cut(s) 153, 303, 453, 744, 843, 927, 1062
BstMBI GATC 5 cut(s) 16, 55, 1014, 1567, 1573
BstMCI CGRYCG 1 cut(s) 1570
BstMWI GCNNNNNNNGC 8 cut(s) 95, 276, 624, 1211, 1451, 1847, 2048, 2148
BstNI CCWGG 1 cut(s) 2127
BstSCI CCNGG 1 cut(s) 2125
BstSFI CTRYAG 2 cut(s) 1584, 2218
BstSLI GKGCMC 1 cut(s) 1035
BstSNI TACGTA 1 cut(s) 955
BstUI CGCG 1 cut(s) 2140
BstV1I GCAGC 6 cut(s) 207, 357, 507, 1072, 1766, 1834
BstXI CCANNNNNNTGG 1 cut(s) 2270
BstZ17I GTATAC 1 cut(s) 1720
Bsu36I CCTNAGG 2 cut(s) 996, 1875
BsuRI GGCC 2 cut(s) 1246, 2215
BtsCI GGATG 7 cut(s) 170, 196, 470, 1384, 1892, 2044, 2045
BtsIMutI CAGTG 2 cut(s) 1290, 2241
CaiI CAGNNNCTG 1 cut(s) 1782
CciI TCATGA 1 cut(s) 1971
CfoI GCGC 3 cut(s) 858, 1768, 2142
Cfr10I RCCGGY 1 cut(s) 69
Cfr13I GGNCC 3 cut(s) 1637, 2214, 2254
Csp6I GTAC 4 cut(s) 1069, 1296, 1649, 1871
CspAI ACCGGT 1 cut(s) 69
CviAII CATG 3 cut(s) 53, 1972, 1991
CviQI GTAC 4 cut(s) 1069, 1296, 1649, 1871
DpnI GATC 5 cut(s) 18, 57, 1016, 1569, 1575
DpnII GATC 5 cut(s) 16, 55, 1014, 1567, 1573
Eam1104I CTCTTC 3 cut(s) 960, 1499, 1860
EarI CTCTTC 3 cut(s) 960, 1499, 1860
Eco105I TACGTA 1 cut(s) 955
Eco130I CCWWGG 1 cut(s) 110
Eco24I GRGCYC 2 cut(s) 1447, 1616
Eco31I GGTCTC 3 cut(s) 153, 303, 453
Eco32I GATATC 2 cut(s) 1003, 1882
Eco47I GGWCC 2 cut(s) 1637, 2254
Eco57I CTGAAG 4 cut(s) 111, 120, 1122, 1674
Eco81I CCTNAGG 2 cut(s) 996, 1875
EcoNI CCTNNNNNAGG 1 cut(s) 1139
EcoRII CCWGG 1 cut(s) 2125
EcoRV GATATC 2 cut(s) 1003, 1882
EcoT14I CCWWGG 1 cut(s) 110
EcoT22I ATGCAT 1 cut(s) 1468
EcoT38I GRGCYC 2 cut(s) 1447, 1616
ErhI CCWWGG 1 cut(s) 110
Esp3I CGTCTC 2 cut(s) 744, 843
FaeI CATG 3 cut(s) 56, 1975, 1994
FalI AAGNNNNNCTT 4 cut(s) 1064, 1096, 1669, 1701
FaqI GGGAC 3 cut(s) 564, 1277, 2187
FatI CATG 3 cut(s) 52, 1971, 1990
FauI CCCGC 1 cut(s) 811
FbaI TGATCA 2 cut(s) 55, 1014
FblI GTMKAC 2 cut(s) 1719, 1826
Fnu4HI GCNGC 6 cut(s) 221, 371, 521, 1086, 1780, 1848
FokI GGATG 7 cut(s) 157, 203, 457, 1371, 1879, 2032, 2051
FriOI GRGCYC 2 cut(s) 1447, 1616
Fsp4HI GCNGC 6 cut(s) 221, 371, 521, 1086, 1780, 1848
FspBI CTAG 5 cut(s) 111, 224, 524, 681, 2103
GlaI GCGC 3 cut(s) 857, 1767, 2141
GluI GCNGC 6 cut(s) 221, 371, 521, 1086, 1780, 1848
GsaI CCCAGC 3 cut(s) 764, 813, 890
GsuI CTGGAG 1 cut(s) 1716
HaeII RGCGCY 1 cut(s) 859
HaeIII GGCC 2 cut(s) 1246, 2215
HapII CCGG 5 cut(s) 70, 188, 1640, 1942, 2060
HhaI GCGC 3 cut(s) 858, 1768, 2142
Hin1II CATG 3 cut(s) 56, 1975, 1994
Hin6I GCGC 3 cut(s) 856, 1766, 2140
HinP1I GCGC 3 cut(s) 856, 1766, 2140
HincII GTYRAC 3 cut(s) 405, 555, 1827
HindII GTYRAC 3 cut(s) 405, 555, 1827
HindIII AAGCTT 2 cut(s) 102, 1801
HpaII CCGG 5 cut(s) 70, 188, 1640, 1942, 2060
HphI GGTGA 6 cut(s) 648, 1439, 1608, 1819, 1825, 1966
Hpy166II GTNNAC 6 cut(s) 255, 405, 555, 1720, 1827, 2123
Hpy188I TCNGA 9 cut(s) 1102, 1188, 1471, 1485, 1734, 1752, 2155, 2278, 2297
Hpy8I GTNNAC 6 cut(s) 255, 405, 555, 1720, 1827, 2123
HpyAV CCTTC 7 cut(s) 41, 1156, 1398, 1649, 1695, 1804, 1935
HpyCH4III ACNGT 3 cut(s) 1030, 1348, 2135
HpyCH4IV ACGT 3 cut(s) 954, 1067, 1579
HpyCH4V TGCA 7 cut(s) 1277, 1466, 1532, 1586, 1670, 1850, 2162
HpyF10VI GCNNNNNNNGC 8 cut(s) 95, 276, 624, 1211, 1451, 1847, 2048, 2148
HpySE526I ACGT 3 cut(s) 954, 1067, 1579
Hsp92II CATG 3 cut(s) 56, 1975, 1994
HspAI GCGC 3 cut(s) 856, 1766, 2140
Kpn2I TCCGGA 3 cut(s) 187, 1639, 1941
KpnI GGTACC 1 cut(s) 1874
Ksp22I TGATCA 2 cut(s) 55, 1014
Kzo9I GATC 5 cut(s) 16, 55, 1014, 1567, 1573
LmnI GCTCC 1 cut(s) 1338
Lsp1109I GCAGC 6 cut(s) 207, 357, 507, 1072, 1766, 1834
LweI GCATC 5 cut(s) 17, 1357, 1453, 1475, 2029
MaeI CTAG 5 cut(s) 111, 224, 524, 681, 2103
MaeII ACGT 3 cut(s) 954, 1067, 1579
MaeIII GTNAC 4 cut(s) 1147, 1505, 1630, 2113
MalI GATC 5 cut(s) 18, 57, 1016, 1569, 1575
MbiI CCGCTC 1 cut(s) 1890
MboI GATC 5 cut(s) 16, 55, 1014, 1567, 1573
MfeI CAATTG 1 cut(s) 2249
MhlI GDGCHC 3 cut(s) 1035, 1447, 1616
MluCI AATT 7 cut(s) 77, 918, 1173, 1358, 1486, 1724, 2249
MlyI GAGTC 8 cut(s) 394, 1481, 1502, 1637, 1832, 1916, 2169, 2193
MmeI TCCRAC 4 cut(s) 737, 1416, 1570, 1990
Mph1103I ATGCAT 1 cut(s) 1468
MroI TCCGGA 3 cut(s) 187, 1639, 1941
MseI TTAA 8 cut(s) 204, 354, 504, 870, 1323, 1458, 1601, 1676
MspA1I CMGCKG 1 cut(s) 1779
MspCI CTTAAG 1 cut(s) 1600
MspI CCGG 5 cut(s) 70, 188, 1640, 1942, 2060
MspR9I CCNGG 1 cut(s) 2127
MunI CAATTG 1 cut(s) 2249
Mva1269I GAATGC 2 cut(s) 1521, 1850
MvaI CCWGG 1 cut(s) 2127
MvnI CGCG 1 cut(s) 2140
MwoI GCNNNNNNNGC 8 cut(s) 95, 276, 624, 1211, 1451, 1847, 2048, 2148
NdeII GATC 5 cut(s) 16, 55, 1014, 1567, 1573
NlaIII CATG 3 cut(s) 56, 1975, 1994
NlaIV GGNNCC 1 cut(s) 1872
NmuCI GTSAC 2 cut(s) 1147, 1505
NsiI ATGCAT 1 cut(s) 1468
PagI TCATGA 1 cut(s) 1971
PcsI WCGNNNNNNNCGW 2 cut(s) 574, 1576
PctI GAATGC 2 cut(s) 1521, 1850
PflFI GACNNNGTC 1 cut(s) 1268
PinAI ACCGGT 1 cut(s) 69
PkrI GCNGC 6 cut(s) 222, 372, 522, 1087, 1781, 1849
Ple19I CGATCG 1 cut(s) 1570
PleI GAGTC 8 cut(s) 393, 1480, 1502, 1637, 1831, 1915, 2169, 2193
PpsI GAGTC 8 cut(s) 393, 1480, 1502, 1637, 1831, 1915, 2169, 2193
Ppu21I YACGTR 1 cut(s) 955
PshBI ATTAAT 1 cut(s) 1323
PsiI TTATAA 1 cut(s) 1179
Psp1406I AACGTT 1 cut(s) 1579
Psp6I CCWGG 1 cut(s) 2125
PspFI CCCAGC 3 cut(s) 760, 809, 886
PspGI CCWGG 1 cut(s) 2125
PspN4I GGNNCC 1 cut(s) 1872
PspPI GGNCC 3 cut(s) 1637, 2214, 2254
PstI CTGCAG 1 cut(s) 1588
PstNI CAGNNNCTG 1 cut(s) 1782
PsyI GACNNNGTC 1 cut(s) 1268
PvuI CGATCG 1 cut(s) 1570
PvuII CAGCTG 1 cut(s) 1779
RsaI GTAC 4 cut(s) 1070, 1297, 1650, 1872
RsaNI GTAC 4 cut(s) 1069, 1296, 1649, 1871
SalI GTCGAC 1 cut(s) 1825
SaqAI TTAA 8 cut(s) 204, 354, 504, 870, 1323, 1458, 1601, 1676
SatI GCNGC 6 cut(s) 221, 371, 521, 1086, 1780, 1848
Sau3AI GATC 5 cut(s) 16, 55, 1014, 1567, 1573
Sau96I GGNCC 3 cut(s) 1637, 2214, 2254
ScaI AGTACT 1 cut(s) 1297
SchI GAGTC 8 cut(s) 394, 1481, 1502, 1637, 1832, 1916, 2169, 2193
ScrFI CCNGG 1 cut(s) 2127
SduI GDGCHC 3 cut(s) 1035, 1447, 1616
SfaNI GCATC 5 cut(s) 17, 1357, 1453, 1475, 2029
SfcI CTRYAG 2 cut(s) 1584, 2218
SinI GGWCC 2 cut(s) 1637, 2254
SmlI CTYRAG 2 cut(s) 1600, 2177
SmoI CTYRAG 2 cut(s) 1600, 2177
SnaBI TACGTA 1 cut(s) 955
SpeI ACTAGT 1 cut(s) 2102
Sse9I AATT 7 cut(s) 77, 918, 1173, 1358, 1486, 1724, 2249
SsiI CCGC 4 cut(s) 804, 1888, 2049, 2138
SspI AATATT 3 cut(s) 246, 396, 546
SspMI CTAG 5 cut(s) 111, 224, 524, 681, 2103
StyD4I CCNGG 1 cut(s) 2125
StyI CCWWGG 1 cut(s) 110
TaaI ACNGT 3 cut(s) 1030, 1348, 2135
TaiI ACGT 3 cut(s) 957, 1070, 1582
TaqI TCGA 5 cut(s) 1566, 1570, 1826, 1964, 1984
TasI AATT 7 cut(s) 77, 918, 1173, 1358, 1486, 1724, 2249
TatI WGTACW 1 cut(s) 1295
Tru1I TTAA 8 cut(s) 204, 354, 504, 870, 1323, 1458, 1601, 1676
Tru9I TTAA 8 cut(s) 204, 354, 504, 870, 1323, 1458, 1601, 1676
TscAI CASTG 2 cut(s) 1290, 2241
TseFI GTSAC 2 cut(s) 1147, 1505
TseI GCWGC 6 cut(s) 220, 370, 520, 1085, 1779, 1847
Tsp45I GTSAC 2 cut(s) 1147, 1505
TspDTI ATGAA 8 cut(s) 156, 306, 456, 963, 1960, 2034, 2175, 2230
TspGWI ACGGA 3 cut(s) 726, 825, 1844
TspRI CASTG 2 cut(s) 1290, 2241
Tth111I GACNNNGTC 1 cut(s) 1268
Vha464I CTTAAG 1 cut(s) 1600
VpaK11BI GGWCC 2 cut(s) 1637, 2254
VspI ATTAAT 1 cut(s) 1323
XagI CCTNNNNNAGG 1 cut(s) 1139
XapI RAATTY 4 cut(s) 77, 1173, 1358, 1486
XbaI TCTAGA 1 cut(s) 680
XcmI CCANNNNNNNNNTGG 3 cut(s) 141, 396, 441
XmaJI CCTAGG 1 cut(s) 110
XmiI GTMKAC 2 cut(s) 1719, 1826
XspI CTAG 5 cut(s) 111, 224, 524, 681, 2103
ZrmI AGTACT 1 cut(s) 1297
Zsp2I ATGCAT 1 cut(s) 1468
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.