Rw4G037360

RNA helicase SDE3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0000595
Physical Location & Seq
Reverse (-)
64002126 .. 64004577
2452 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G037360.1

Sequence Viewer

Length: 2022 bp
ATGTCAGTCTTTTTTGATCTTCTGAGATGCCTTCTGTGTTGTTCTGATGAGCCTGATTTTCATCGAACTTCTTCCTGTTATACAAGAAACCGTTTTGTTGATACCGCTGACCTTCACTATGAGTATGATCCCATTCCCAGTCCCAGTCCCAGGCGCAGCCATTTGCCAACTTCAACTTCATTTCAGCCTCAGTACTATTCTCCTGATTTTGTTAATTCTCTACTTCATTCTCCTGCTTCACCTCCCACCTCCTCTTATGAACCACCACCTCAATCAACAACACCACCACCAGCATCTCAATATTATTCTTCTAATAACAAACCGCCGGTCACTTCTTTTATCAGTTCATCTTCATCATCTAAACCAACCCTATCGTCTACTATTAAACCATCTTCATCATCCTCTAAGAAGCCACCTCAGCCAACATCATCTAATTCTAAACCAAATCCATCCTCCCAAAAACCTCCATCACATTCTTCATCTCCATCTTCTCCAAAGCTCCCTCCTGTCTACAAGTCAATTCTATCCCCAGCCTCCTCCTCCTCCTCCTTCTCCCCAAATGTTACAAGTCAACACCAACAAGACGGGAAGAAAACAGCTTATGTGTGTGTTGAAGAGGATCCATTACCTGTATTCATGATTCCTGAGGATATCAAAGCCTTGATCAAGAACAATATTGTGCCCAAAGTTCTGATTCGTCCATTATCCCCCACAACATACAAGGACTACTTTGCTGCTCTCTTATATTCTGAAGATTTCTACTTGGAGCAATGGAGTGATTTCCTTTTGAAGGGTGTGACATTGGCGTTGGATGAAAGTTCAATTTATAAAATTCGGAAAAAGGAAGCAATTTATAAAAATCGGAAAAAGGAAGATAAGGAAGATAAAATCTTTGTAGCATTTGAGCTTGATTCTGTTCCTGAGGAACGGCCATACCTCTTATCAAGGGACTTGGTCTTTGCACGACCAGTGGATAACAGTACTGCTGAACCCTTTAAGGGCTTCATCTATAAAGTTGTTGGGAGCAAACGCGTTTTGGTTGAATTTGAAGATGGCTTTTATTCTAATCATCATTCCAACAAAAAATATGATATCAGCTTCTCATTCAATAGAGTTTGTTTGAAAAGAGCCCACCATGCAGTTAAAGCTGCATCAGACGCCTTGTTTCAGAACTTCCTCTTCCCTGACACTGACTCACGAGCAAGCACTCCTACTGCACCAGCTCTGCTTTCTACTACCTGTCATAAGCTTGATCAAGATCAACGTTGTGCGGTTGGACATATCTTAAGGATTCAGGGCTCACCACCTTATGTAGTAACTGGTCCGGACTGTGAGGGCGAATCTTTTGCATGTTTAAGAGAACCTTCAAAAACTGAAGGCAAATCTTTTGCATATTTAAGAGAACCTTCAAAAACTGGAGTGGTTGTTAGTGAAGCAGTATATCAATTATGTCAGAAGTCGCCAAAGTATCGGATTCTTATCTGTGCGCCTACTAACAGCTGCTGCGATGTGCTGATGAGAAGCTTGGTGAAGGTGATTCCAGAGTCAACTATGTTTCGTGCCAATGCTGCATTCCGTGAGAAAGAAGAGGTACCTGAGGATATCCTCCGCTCATCCCTTTACAAGGAGTCTTGTTTTTCTTGTCCTTCAATAGATAAACTCCGGAAATACAGGGTGATTTTCTCGACTTTCATGAGTAGCTTTCGACTACATGATAAAGGCATAGCTATTGGACATTTTAGCCATATTTTTCTGGTGGATGCTTCATCCGCTATTGAACCAGAAACATTGGTCGCCCTGACCAACTTTGCTGACAAGACTACTAGTGTTATAGTTACTGGTAAACCTGGAAACAGTCCGCGCTGGGTTCGCGCTGACATTGCAAGTAAAAAGGGACTCAAGATTTCGTACTTTGAAAGACTCTGTAAGCTCAGCCCCTATAGAAGCCGCAGTGCGATGTTCATCACACAATTAGACCTGAACCAAAAGTCAGGCTCAGACTACCAAACAAGAACTTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

673

Amino Acids

75.38

Weight (kDa)

8.93

Isoelectric Point (pI)

61.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MOV-10_beta-barrel PF21634 291 - 367 1.3e-09 Helicase MOV-10, beta-barrel domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000595)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g37100 FvH4_4g37100 FvH4_4g37100
malus_domestica MD13G1238300.v1.1 MD16G1243100.v1.1 MD16G1243200.v1.1
prunus_persica Prupe.1G070100_v2.0.a1 Prupe.1G070200_v2.0.a1 Prupe.1G070300_v2.0.a1 Prupe.1G070300_v2.0.a1
pyrus_communis pycom13g21070 pycom16g20390 pycom16g20400
rosa_chinensis RchiOBHm_Chr1g0318071 RchiOBHm_Chr1g0318111 RchiOBHm_Chr4g0446861 RchiOBHm_Chr4g0446881 RchiOBHm_Chr4g0446891
rosa_laevigata RLG00000005623 RLG00000005624 RLG00000009337 RLG00000030560 RLG00000030566
rosa_multiflora Rmu_co8177450.1_g000001 Rmu_sc0001753.1_g000012 Rmu_sc0001753.1_g000013 Rmu_sc0001753.1_g000014 Rmu_sc0001753.1_g000015 Rmu_sc0011424.1_g000009 Rmu_sc0011424.1_g000020 Rmu_sc0034555.1_g000001 Rmu_ssc0000008.1_g000002
rosa_roxburghii Rroxscaffold_4G00329530 Rroxscaffold_4G00329590 Rroxscaffold_4G00329800 Rroxscaffold_4G00329810 Rroxscaffold_4G00329860 Rroxscaffold_4G00329910 Rroxscaffold_4G00330030 Rroxscaffold_5G00343610 Rroxscaffold_5G00348280 Rroxscaffold_5G00387220 Rroxscaffold_5G00387230
rosa_rugosa Rorug01G0019100 Rorug01G0019100 Rorug01G0019500 Rorug01G0019600 Rorug04G0010100 Rorug04G0375200 Rorug04G0375300 Rorug04G0375400 Rorug04G0375500 Rorug04G0375600 Rorug04G0375700
rosa_samantha Rh1AG030700 Rh1AG030900 Rh1BG026900 Rh1CG029500 Rh1CG029700 Rh1DG040500 Rh4AG088100 Rh4AG436700 Rh4AG436800 Rh4AG436900 Rh4BG084700 Rh4BG445800 Rh4BG445900 Rh4BG446100 Rh4CG096300 Rh4CG463100 Rh4CG463200 Rh4CG463400 Rh4DG444500 Rh4DG444600 Rh4DG444700
rosa_wichuraiana Rw1G002420 Rw1G002440 Rw1G002480 Rw4G037360 Rw4G037370 Rw4G037380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 828, 855
Acc65I GGTACC 1 cut(s) 1591
AccB1I GGYRCC 1 cut(s) 1591
AccBSI CCGCTC 1 cut(s) 1611
AccI GTMKAC 2 cut(s) 377, 510
AccII CGCG 3 cut(s) 1032, 1861, 1872
AccIII TCCGGA 2 cut(s) 1324, 1662
AciI CCGC 7 cut(s) 105, 323, 1271, 1609, 1770, 1859, 1948
AclI AACGTT 1 cut(s) 1264
AclWI GGATC 3 cut(s) 122, 614, 627
AcoI YGGCCR 1 cut(s) 929
AcsI RAATTY 2 cut(s) 831, 1043
AcuI CTGAAG 2 cut(s) 771, 1395
AcyI GRCGYC 1 cut(s) 1158
AfaI GTAC 4 cut(s) 194, 982, 1593, 1910
AfiI CCNNNNNNNGG 4 cut(s) 150, 790, 998, 1624
AflII CTTAAG 1 cut(s) 1285
AflIII ACRYGT 1 cut(s) 1030
AhlI ACTAGT 1 cut(s) 1823
AjnI CCWGG 2 cut(s) 149, 1846
AjuI GAANNNNNNNTTGG 4 cut(s) 160, 192, 1556, 1588
AlwI GGATC 3 cut(s) 122, 614, 627
AlwNI CAGNNNCTG 1 cut(s) 1503
Aor13HI TCCGGA 2 cut(s) 1324, 1662
AoxI GGCC 1 cut(s) 929
ApeKI GCWGC 6 cut(s) 156, 734, 1148, 1500, 1503, 1568
ApoI RAATTY 2 cut(s) 831, 1043
ArsI GACNNNNNNTTYG 4 cut(s) 312, 344, 941, 973
Asp700I GAANNNNTTC 1 cut(s) 70
Asp718I GGTACC 1 cut(s) 1591
AspLEI GCGC 4 cut(s) 156, 1489, 1863, 1874
AspS9I GGNCC 1 cut(s) 1322
AsuHPI GGTGA 5 cut(s) 231, 1293, 1540, 1546, 1687
AvaII GGWCC 1 cut(s) 1322
AxyI CCTNAGG 3 cut(s) 645, 921, 1596
BaeGI GKGCMC 1 cut(s) 684
BamHI GGATCC 1 cut(s) 619
BanI GGYRCC 1 cut(s) 1591
BanII GRGCYC 2 cut(s) 1132, 1301
BauI CACGAG 1 cut(s) 1197
BbvCI CCTCAGC 1 cut(s) 417
BbvI GCAGC 6 cut(s) 168, 721, 1135, 1487, 1490, 1555
BccI CCATC 5 cut(s) 397, 457, 475, 493, 1046
BceAI ACGGC 1 cut(s) 944
BcgI CGANNNNNNTGC 2 cut(s) 1328, 1362
BciT130I CCWGG 2 cut(s) 151, 1848
BclI TGATCA 2 cut(s) 663, 1252
BcuI ACTAGT 1 cut(s) 1823
BfaI CTAG 1 cut(s) 1824
BfmI CTRYAG 1 cut(s) 1939
BfrI CTTAAG 1 cut(s) 1285
BisI GCNGC 7 cut(s) 157, 735, 1149, 1501, 1504, 1569, 1948
BlpI GCTNAGC 1 cut(s) 1931
BlsI GCNGC 7 cut(s) 158, 736, 1150, 1502, 1505, 1570, 1949
BmcAI AGTACT 2 cut(s) 194, 982
Bme1390I CCNGG 2 cut(s) 151, 1848
Bme18I GGWCC 1 cut(s) 1322
BmgT120I GGNCC 1 cut(s) 1322
BmiI GGNNCC 2 cut(s) 621, 1593
BmrFI CCNGG 2 cut(s) 151, 1848
BmrI ACTGGG 2 cut(s) 132, 138
BmsI GCATC 4 cut(s) 17, 302, 1160, 1750
BmuI ACTGGG 2 cut(s) 132, 138
BpmI CTGGAG 1 cut(s) 1437
Bpu10I CCTNAGC 1 cut(s) 417
Bpu1102I GCTNAGC 1 cut(s) 1931
BpuEI CTTGAG 1 cut(s) 1883
BsaBI GATNNNNATC 4 cut(s) 60, 1257, 1478, 1961
BsaHI GRCGYC 1 cut(s) 1158
BsaJI CCNNGG 1 cut(s) 149
BsaWI WCCGGW 2 cut(s) 1324, 1662
BsaXI ACNNNNNCTCC 2 cut(s) 1410, 1440
Bsc4I CCNNNNNNNGG 4 cut(s) 150, 790, 998, 1624
Bse118I RCCGGY 1 cut(s) 325
Bse1I ACTGG 6 cut(s) 138, 144, 968, 1324, 1420, 1843
Bse21I CCTNAGG 3 cut(s) 645, 921, 1596
Bse3DI GCAATG 2 cut(s) 776, 1878
Bse8I GATNNNNATC 4 cut(s) 60, 1257, 1478, 1961
BseAI TCCGGA 2 cut(s) 1324, 1662
BseBI CCWGG 2 cut(s) 151, 1848
BseDI CCNNGG 1 cut(s) 149
BseGI GGATG 6 cut(s) 398, 449, 817, 1613, 1765, 1766
BseJI GATNNNNATC 4 cut(s) 60, 1257, 1478, 1961
BseLI CCNNNNNNNGG 4 cut(s) 150, 790, 998, 1624
BseMI GCAATG 2 cut(s) 776, 1878
BseMII CTCAG 8 cut(s) 14, 203, 431, 636, 912, 1587, 1945, 2010
BseNI ACTGG 6 cut(s) 138, 144, 968, 1324, 1420, 1843
BseRI GAGGAG 5 cut(s) 241, 526, 529, 532, 535
BseSI GKGCMC 1 cut(s) 684
BseXI GCAGC 6 cut(s) 168, 721, 1135, 1487, 1490, 1555
BseYI CCCAGC 2 cut(s) 529, 1863
BsgI GTGCAG 1 cut(s) 1200
Bsh1236I CGCG 3 cut(s) 1032, 1861, 1872
BshFI GGCC 1 cut(s) 931
BshNI GGYRCC 1 cut(s) 1591
BsiSI CCGG 3 cut(s) 326, 1325, 1663
BslFI GGGAC 4 cut(s) 126, 132, 962, 1908
BslI CCNNNNNNNGG 4 cut(s) 150, 790, 998, 1624
BsmFI GGGAC 4 cut(s) 126, 132, 962, 1908
BsmI GAATGC 1 cut(s) 1571
BsnI GGCC 1 cut(s) 931
Bsp1286I GDGCHC 3 cut(s) 684, 1132, 1301
Bsp13I TCCGGA 2 cut(s) 1324, 1662
Bsp143I GATC 6 cut(s) 16, 127, 619, 663, 1252, 1258
Bsp1720I GCTNAGC 1 cut(s) 1931
BspACI CCGC 7 cut(s) 105, 323, 1271, 1609, 1770, 1859, 1948
BspANI GGCC 1 cut(s) 931
BspCNI CTCAG 8 cut(s) 15, 202, 430, 637, 913, 1588, 1944, 2009
BspEI TCCGGA 2 cut(s) 1324, 1662
BspFNI CGCG 3 cut(s) 1032, 1861, 1872
BspHI TCATGA 2 cut(s) 636, 1692
BspLI GGNNCC 2 cut(s) 621, 1593
BspPI GGATC 3 cut(s) 122, 614, 627
BspT107I GGYRCC 1 cut(s) 1591
BspTI CTTAAG 1 cut(s) 1285
BsrBI CCGCTC 1 cut(s) 1611
BsrDI GCAATG 2 cut(s) 776, 1878
BsrFI RCCGGY 1 cut(s) 325
BsrI ACTGG 6 cut(s) 138, 144, 968, 1324, 1420, 1843
BssAI RCCGGY 1 cut(s) 325
BssECI CCNNGG 1 cut(s) 149
BssMI GATC 6 cut(s) 16, 127, 619, 663, 1252, 1258
BssNI GRCGYC 1 cut(s) 1158
BssSI CACGAG 1 cut(s) 1197
Bst2BI CACGAG 1 cut(s) 1197
Bst2UI CCWGG 2 cut(s) 151, 1848
Bst4CI ACNGT 4 cut(s) 92, 980, 1331, 1856
Bst6I CTCTTC 3 cut(s) 609, 1184, 1581
BstACI GRCGYC 1 cut(s) 1158
BstAFI CTTAAG 1 cut(s) 1285
BstC8I GCNNGC 1 cut(s) 1204
BstDEI CTNAG 9 cut(s) 23, 189, 405, 417, 645, 921, 1596, 1931, 1996
BstENI CCTNNNNNAGG 2 cut(s) 788, 1622
BstF5I GGATG 6 cut(s) 398, 449, 817, 1613, 1765, 1766
BstFNI CGCG 3 cut(s) 1032, 1861, 1872
BstHHI GCGC 4 cut(s) 156, 1489, 1863, 1874
BstKTI GATC 6 cut(s) 19, 130, 622, 666, 1255, 1261
BstMBI GATC 6 cut(s) 16, 127, 619, 663, 1252, 1258
BstMWI GCNNNNNNNGC 8 cut(s) 418, 1136, 1145, 1157, 1568, 1769, 1869, 1880
BstNI CCWGG 2 cut(s) 151, 1848
BstNSI RCATGY 1 cut(s) 1353
BstSCI CCNGG 2 cut(s) 149, 1846
BstSFI CTRYAG 1 cut(s) 1939
BstSLI GKGCMC 1 cut(s) 684
BstUI CGCG 3 cut(s) 1032, 1861, 1872
BstV1I GCAGC 6 cut(s) 168, 721, 1135, 1487, 1490, 1555
BstX2I RGATCY 1 cut(s) 619
BstYI RGATCY 1 cut(s) 619
Bsu36I CCTNAGG 3 cut(s) 645, 921, 1596
BsuRI GGCC 1 cut(s) 931
BtgZI GCGATG 2 cut(s) 1521, 1970
BtsCI GGATG 6 cut(s) 398, 449, 817, 1613, 1765, 1766
BtsI GCAGTG 1 cut(s) 1957
BtsIMutI CAGTG 3 cut(s) 975, 1188, 1957
Cac8I GCNNGC 1 cut(s) 1204
CaiI CAGNNNCTG 1 cut(s) 1503
CciI TCATGA 2 cut(s) 636, 1692
CfoI GCGC 4 cut(s) 156, 1489, 1863, 1874
Cfr10I RCCGGY 1 cut(s) 325
Cfr13I GGNCC 1 cut(s) 1322
CseI GACGC 1 cut(s) 1166
Csp6I GTAC 4 cut(s) 193, 981, 1592, 1909
CviAII CATG 5 cut(s) 637, 1136, 1350, 1693, 1712
CviQI GTAC 4 cut(s) 193, 981, 1592, 1909
DdeI CTNAG 9 cut(s) 23, 189, 405, 417, 645, 921, 1596, 1931, 1996
DpnI GATC 6 cut(s) 18, 129, 621, 665, 1254, 1260
DpnII GATC 6 cut(s) 16, 127, 619, 663, 1252, 1258
EaeI YGGCCR 1 cut(s) 929
Eam1104I CTCTTC 3 cut(s) 609, 1184, 1581
EarI CTCTTC 3 cut(s) 609, 1184, 1581
Eco24I GRGCYC 2 cut(s) 1132, 1301
Eco32I GATATC 3 cut(s) 652, 1093, 1603
Eco47I GGWCC 1 cut(s) 1322
Eco57I CTGAAG 2 cut(s) 771, 1395
Eco81I CCTNAGG 3 cut(s) 645, 921, 1596
EcoNI CCTNNNNNAGG 2 cut(s) 788, 1622
EcoRII CCWGG 2 cut(s) 149, 1846
EcoRV GATATC 3 cut(s) 652, 1093, 1603
EcoT38I GRGCYC 2 cut(s) 1132, 1301
FaeI CATG 5 cut(s) 640, 1139, 1353, 1696, 1715
FalI AAGNNNNNCTT 6 cut(s) 713, 745, 1348, 1380, 1390, 1422
FaqI GGGAC 4 cut(s) 126, 132, 962, 1908
FatI CATG 5 cut(s) 636, 1135, 1349, 1692, 1711
FbaI TGATCA 2 cut(s) 663, 1252
FblI GTMKAC 2 cut(s) 377, 510
Fnu4HI GCNGC 7 cut(s) 157, 735, 1149, 1501, 1504, 1569, 1948
FokI GGATG 6 cut(s) 385, 436, 824, 1600, 1753, 1772
FriOI GRGCYC 2 cut(s) 1132, 1301
Fsp4HI GCNGC 7 cut(s) 157, 735, 1149, 1501, 1504, 1569, 1948
FspBI CTAG 1 cut(s) 1824
GlaI GCGC 4 cut(s) 155, 1488, 1862, 1873
GluI GCNGC 7 cut(s) 157, 735, 1149, 1501, 1504, 1569, 1948
GsaI CCCAGC 2 cut(s) 533, 1867
GsuI CTGGAG 1 cut(s) 1437
HaeIII GGCC 1 cut(s) 931
HapII CCGG 3 cut(s) 326, 1325, 1663
HgaI GACGC 1 cut(s) 1166
HhaI GCGC 4 cut(s) 156, 1489, 1863, 1874
Hin1I GRCGYC 1 cut(s) 1158
Hin1II CATG 5 cut(s) 640, 1139, 1353, 1696, 1715
Hin6I GCGC 4 cut(s) 154, 1487, 1861, 1872
HinP1I GCGC 4 cut(s) 154, 1487, 1861, 1872
HincII GTYRAC 2 cut(s) 572, 1548
HindII GTYRAC 2 cut(s) 572, 1548
HindIII AAGCTT 2 cut(s) 1247, 1522
HpaII CCGG 3 cut(s) 326, 1325, 1663
HphI GGTGA 5 cut(s) 231, 1293, 1540, 1546, 1687
Hpy166II GTNNAC 5 cut(s) 378, 511, 572, 1548, 1844
Hpy8I GTNNAC 5 cut(s) 378, 511, 572, 1548, 1844
HpyAV CCTTC 9 cut(s) 41, 122, 559, 784, 1370, 1374, 1416, 1525, 1656
HpyCH4III ACNGT 4 cut(s) 92, 980, 1331, 1856
HpyCH4IV ACGT 1 cut(s) 1264
HpyCH4V TGCA 8 cut(s) 962, 1139, 1151, 1217, 1349, 1391, 1571, 1883
HpyF10VI GCNNNNNNNGC 8 cut(s) 418, 1136, 1145, 1157, 1568, 1769, 1869, 1880
HpyF3I CTNAG 9 cut(s) 23, 189, 405, 417, 645, 921, 1596, 1931, 1996
HpySE526I ACGT 1 cut(s) 1264
Hsp92I GRCGYC 1 cut(s) 1158
Hsp92II CATG 5 cut(s) 640, 1139, 1353, 1696, 1715
HspAI GCGC 4 cut(s) 154, 1487, 1861, 1872
Kpn2I TCCGGA 2 cut(s) 1324, 1662
KpnI GGTACC 1 cut(s) 1595
Ksp22I TGATCA 2 cut(s) 663, 1252
Kzo9I GATC 6 cut(s) 16, 127, 619, 663, 1252, 1258
LmnI GCTCC 3 cut(s) 504, 766, 1023
Lsp1109I GCAGC 6 cut(s) 168, 721, 1135, 1487, 1490, 1555
LweI GCATC 4 cut(s) 17, 302, 1160, 1750
MaeI CTAG 1 cut(s) 1824
MaeII ACGT 1 cut(s) 1264
MaeIII GTNAC 5 cut(s) 328, 562, 796, 1315, 1834
MalI GATC 6 cut(s) 18, 129, 621, 665, 1254, 1260
MbiI CCGCTC 1 cut(s) 1611
MboI GATC 6 cut(s) 16, 127, 619, 663, 1252, 1258
MflI RGATCY 1 cut(s) 619
MhlI GDGCHC 3 cut(s) 684, 1132, 1301
MluCI AATT 9 cut(s) 214, 433, 519, 822, 831, 849, 1043, 1445, 1970
MluI ACGCGT 1 cut(s) 1030
MlyI GAGTC 5 cut(s) 1187, 1553, 1637, 1890, 1914
MmeI TCCRAC 3 cut(s) 789, 1101, 1255
MroI TCCGGA 2 cut(s) 1324, 1662
MroXI GAANNNNTTC 1 cut(s) 70
MseI TTAA 7 cut(s) 213, 384, 996, 1143, 1286, 1355, 1397
MspA1I CMGCKG 2 cut(s) 107, 1500
MspCI CTTAAG 1 cut(s) 1285
MspI CCGG 3 cut(s) 326, 1325, 1663
MspR9I CCNGG 2 cut(s) 151, 1848
Mva1269I GAATGC 1 cut(s) 1571
MvaI CCWGG 2 cut(s) 151, 1848
MvnI CGCG 3 cut(s) 1032, 1861, 1872
MwoI GCNNNNNNNGC 8 cut(s) 418, 1136, 1145, 1157, 1568, 1769, 1869, 1880
NdeII GATC 6 cut(s) 16, 127, 619, 663, 1252, 1258
NlaIII CATG 5 cut(s) 640, 1139, 1353, 1696, 1715
NlaIV GGNNCC 2 cut(s) 621, 1593
NmuCI GTSAC 2 cut(s) 328, 796
NspI RCATGY 1 cut(s) 1353
PagI TCATGA 2 cut(s) 636, 1692
PctI GAATGC 1 cut(s) 1571
PdmI GAANNNNTTC 1 cut(s) 70
PfeI GAWTC 7 cut(s) 640, 694, 911, 1291, 1340, 1474, 1537
PflFI GACNNNGTC 1 cut(s) 953
PkrI GCNGC 7 cut(s) 158, 736, 1150, 1502, 1505, 1570, 1949
PleI GAGTC 5 cut(s) 1187, 1552, 1636, 1890, 1914
PpsI GAGTC 5 cut(s) 1187, 1552, 1636, 1890, 1914
PsiI TTATAA 2 cut(s) 828, 855
Psp1406I AACGTT 1 cut(s) 1264
Psp6I CCWGG 2 cut(s) 149, 1846
PspFI CCCAGC 2 cut(s) 529, 1863
PspGI CCWGG 2 cut(s) 149, 1846
PspN4I GGNNCC 2 cut(s) 621, 1593
PspPI GGNCC 1 cut(s) 1322
PstNI CAGNNNCTG 1 cut(s) 1503
PsuI RGATCY 1 cut(s) 619
PsyI GACNNNGTC 1 cut(s) 953
PvuII CAGCTG 1 cut(s) 1500
RsaI GTAC 4 cut(s) 194, 982, 1593, 1910
RsaNI GTAC 4 cut(s) 193, 981, 1592, 1909
SaqAI TTAA 7 cut(s) 213, 384, 996, 1143, 1286, 1355, 1397
SatI GCNGC 7 cut(s) 157, 735, 1149, 1501, 1504, 1569, 1948
Sau3AI GATC 6 cut(s) 16, 127, 619, 663, 1252, 1258
Sau96I GGNCC 1 cut(s) 1322
ScaI AGTACT 2 cut(s) 194, 982
SchI GAGTC 5 cut(s) 1187, 1553, 1637, 1890, 1914
ScrFI CCNGG 2 cut(s) 151, 1848
SduI GDGCHC 3 cut(s) 684, 1132, 1301
SfaNI GCATC 4 cut(s) 17, 302, 1160, 1750
SfcI CTRYAG 1 cut(s) 1939
SinI GGWCC 1 cut(s) 1322
SmlI CTYRAG 2 cut(s) 1285, 1898
SmoI CTYRAG 2 cut(s) 1285, 1898
SpeI ACTAGT 1 cut(s) 1823
Sse9I AATT 9 cut(s) 214, 433, 519, 822, 831, 849, 1043, 1445, 1970
SsiI CCGC 7 cut(s) 105, 323, 1271, 1609, 1770, 1859, 1948
SspI AATATT 2 cut(s) 302, 676
SspMI CTAG 1 cut(s) 1824
StyD4I CCNGG 2 cut(s) 149, 1846
TaaI ACNGT 4 cut(s) 92, 980, 1331, 1856
TaiI ACGT 1 cut(s) 1267
TaqI TCGA 3 cut(s) 64, 1685, 1705
TasI AATT 9 cut(s) 214, 433, 519, 822, 831, 849, 1043, 1445, 1970
TatI WGTACW 2 cut(s) 192, 980
TauI GCSGC 1 cut(s) 1950
TfiI GAWTC 7 cut(s) 640, 694, 911, 1291, 1340, 1474, 1537
Tru1I TTAA 7 cut(s) 213, 384, 996, 1143, 1286, 1355, 1397
Tru9I TTAA 7 cut(s) 213, 384, 996, 1143, 1286, 1355, 1397
TscAI CASTG 3 cut(s) 975, 1195, 1957
TseFI GTSAC 2 cut(s) 328, 796
TseI GCWGC 6 cut(s) 156, 734, 1148, 1500, 1503, 1568
Tsp45I GTSAC 2 cut(s) 328, 796
TspGWI ACGGA 1 cut(s) 1565
TspRI CASTG 3 cut(s) 975, 1195, 1957
Tth111I GACNNNGTC 1 cut(s) 953
Vha464I CTTAAG 1 cut(s) 1285
VpaK11BI GGWCC 1 cut(s) 1322
XagI CCTNNNNNAGG 2 cut(s) 788, 1622
XapI RAATTY 2 cut(s) 831, 1043
XceI RCATGY 1 cut(s) 1353
XmiI GTMKAC 2 cut(s) 377, 510
XmnI GAANNNNTTC 1 cut(s) 70
XspI CTAG 1 cut(s) 1824
ZrmI AGTACT 2 cut(s) 194, 982
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.