Rh4CG463100

RNA helicase SDE3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0000595
Physical Location & Seq
Reverse (-)
69367330 .. 69369781
2452 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG463100.1

Sequence Viewer

Length: 2010 bp
ATGTCATGCTTTTTTGATCTTCTGAGATGCCTTCTGTGTTGTTCTGATGAGCCTGATTTTCATCGAACTTCTTCCGGTTATACAAGAAACCGTTTTGTTGATACCATTGACCTTCACTATGAGTATGATCCCATTCCCAGTCCCAGGCGCAGCCATTTGCCAACTTCTTCAACTTCATTTCAGCCTCAGTACTATTCTCGTGATTTAGTTAATTCTCTACTTCATTCTCCTGCTTCACCTCCCACCTCCTCTTATAAACCACCACCTCAATCAACAACAACACCACCAGCATCTCAATATTATTCATCTTATAATAAACCGCCGGTCACTTCTTTTATCAGTTCATCTTCATCATCGTCTACTATTAAACCATCTTCATCATCCCCTAAGAAGCCACCTCAGCCAACATCATCTAATTCTAAACCAGATCCATCCTCCCAAAAACCTCCATCACATTCTTCATCTCCATCTTTTCCAAAGCTCCCTCCTGTCTACAAGTCAATTCTATCCCCAGCTTCCTCCTCCTCCTCCTCCTCCTTCTCCCCAAATGTTACAAGTCAACACCAACAAGACGGGAAGAAAACAGCTTATGTGTGTGTTGAAGAGGATCCATTACCTGTATTCATGATTCCTGAGGATATCAAAGCCTTGATCAAGAACAAAATTGTGCCCAAAGTTCTGAATCGCGCATTATCCTCCACAACATACAAGGACTACTTTGCCGCTCTCTTATATTCTGAAGATTTCTACTTGGAGCAATGGAGTGATTTCCTTTTGAAGGGTGTGACATTGGCGTTGGATGAAAGTTCAATTTATAAAATTCGGAAAAAGGAAGCAATTTATAAAAATCGGAAAAAGGAAGATAAGGAAGATAAAATCTTTGTAGCATTTGAGCTTGATTCTGTTCCTGAGGAACGGCCATTCCTCTTATCAAGGGACTTGGTCTTTGCACGACCAGTGGGTAAGAGTACTGCTGAACCCTTTAAGGGCTTCATCTATAAAGTTGTTGGGAGCAAACGCGTTTTGGTTGAATTTGAAGATGGCTTTTATTCTAATCATCATTCCAACAAAAAATATGATATCAGCTTCTCATTCAATAGAGTGTGTTTGAAAAGAGCCCACCATGCAGTTAAAGCTGCATCAGACGCCTTGTTTCAGAACTTCCTCTTCCCTGACACTGACTCACGAGCAAGCATTCCTACTGCACCAGCTCTGCTTTCTACTACCTGTCATAAGCTTGATCAAAATCAACGTTGTGCGGTTGGACATATCTTAAGGATTCAGGGCTCACCACCTTATGTAGTAACTGGTCCAGACTGTGAAGGCGAATCTTTTGCATGTTTAAGAGAACCTTCAAAAACTGAAGGCAAATCTTTTGCATATTTAAGAGAACCTTCAAAAACTGGAGTGGTTGTTAGTGAAGCAGTATACCAATTATGTCAGAAGTCGCCAAAGTATCGGATTCTTATCTGTGCGCCTACTAACAGCTGCTGCGATGTGCTGATGAGAAGCTTGGTGAAGGTGATTCCAGAGTCGACTATGTTTCGTGCCAATGCTGCATTCCGTGAGAAAGAAGAGGTACCTGAGGATATCCTCCGCTCATCCCTTTACAAAGAGTCTTGTTTTTCTTGTCCTTCAATAGAGAAACTCCGGAAATACAGGGTGATTTTCTCGACTTTCATGAGTAGCTTTCGACTACATGATAAAGGCATACCTGTTGGACATTTTAGCCATATTTTTCTGGTGGATGCTTCATCCGCTATTGAACCAGAAACATTGGTCGCCCTGACTAACTTTGCTGACAAGACTACTAGTGTTATAGTTACTGGTAAACCTGGAAATAGTCCGCGCTGGGTTCGCGCTGACATTGCAAGGAAAAAGGGACTCAAGATTTCATACTTTGAAAGACTCTGTAAGCTCAGCCCCTATAGAAGCCGCAGTCCAATGTCCATCGCACAATTAGACCTGAACCAAAAGTCATACTCAGACTACCAAACAAGAACTTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

669

Amino Acids

75.15

Weight (kDa)

9.15

Isoelectric Point (pI)

61.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MOV-10_beta-barrel PF21634 287 - 363 1.9e-09 Helicase MOV-10, beta-barrel domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000595)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g37100 FvH4_4g37100 FvH4_4g37100
malus_domestica MD13G1238300.v1.1 MD16G1243100.v1.1 MD16G1243200.v1.1
prunus_persica Prupe.1G070100_v2.0.a1 Prupe.1G070200_v2.0.a1 Prupe.1G070300_v2.0.a1 Prupe.1G070300_v2.0.a1
pyrus_communis pycom13g21070 pycom16g20390 pycom16g20400
rosa_chinensis RchiOBHm_Chr1g0318071 RchiOBHm_Chr1g0318111 RchiOBHm_Chr4g0446861 RchiOBHm_Chr4g0446881 RchiOBHm_Chr4g0446891
rosa_laevigata RLG00000005623 RLG00000005624 RLG00000009337 RLG00000030560 RLG00000030566
rosa_multiflora Rmu_co8177450.1_g000001 Rmu_sc0001753.1_g000012 Rmu_sc0001753.1_g000013 Rmu_sc0001753.1_g000014 Rmu_sc0001753.1_g000015 Rmu_sc0011424.1_g000009 Rmu_sc0011424.1_g000020 Rmu_sc0034555.1_g000001 Rmu_ssc0000008.1_g000002
rosa_roxburghii Rroxscaffold_4G00329530 Rroxscaffold_4G00329590 Rroxscaffold_4G00329800 Rroxscaffold_4G00329810 Rroxscaffold_4G00329860 Rroxscaffold_4G00329910 Rroxscaffold_4G00330030 Rroxscaffold_5G00343610 Rroxscaffold_5G00348280 Rroxscaffold_5G00387220 Rroxscaffold_5G00387230
rosa_rugosa Rorug01G0019100 Rorug01G0019100 Rorug01G0019500 Rorug01G0019600 Rorug04G0010100 Rorug04G0375200 Rorug04G0375300 Rorug04G0375400 Rorug04G0375500 Rorug04G0375600 Rorug04G0375700
rosa_samantha Rh1AG030700 Rh1AG030900 Rh1BG026900 Rh1CG029500 Rh1CG029700 Rh1DG040500 Rh4AG088100 Rh4AG436700 Rh4AG436800 Rh4AG436900 Rh4BG084700 Rh4BG445800 Rh4BG445900 Rh4BG446100 Rh4CG096300 Rh4CG463100 Rh4CG463200 Rh4CG463400 Rh4DG444500 Rh4DG444600 Rh4DG444700
rosa_wichuraiana Rw1G002420 Rw1G002440 Rw1G002480 Rw4G037360 Rw4G037370 Rw4G037380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 4 cut(s) 255, 312, 816, 843
Acc65I GGTACC 1 cut(s) 1579
AccB1I GGYRCC 1 cut(s) 1579
AccBSI CCGCTC 2 cut(s) 725, 1599
AccI GTMKAC 4 cut(s) 359, 492, 1428, 1535
AccII CGCG 4 cut(s) 687, 1020, 1849, 1860
AccIII TCCGGA 1 cut(s) 1650
AciI CCGC 7 cut(s) 320, 723, 1259, 1597, 1758, 1847, 1936
AclI AACGTT 1 cut(s) 1252
AclWI GGATC 4 cut(s) 122, 422, 602, 615
AcoI YGGCCR 1 cut(s) 917
AcsI RAATTY 2 cut(s) 819, 1031
AcuI CTGAAG 2 cut(s) 759, 1383
AcyI GRCGYC 1 cut(s) 1146
AfaI GTAC 3 cut(s) 191, 970, 1581
AfiI CCNNNNNNNGG 3 cut(s) 144, 778, 986
AflII CTTAAG 1 cut(s) 1273
AflIII ACRYGT 1 cut(s) 1018
AhlI ACTAGT 1 cut(s) 1811
AjnI CCWGG 2 cut(s) 143, 1834
AjuI GAANNNNNNNTTGG 2 cut(s) 1544, 1576
AloI GAACNNNNNNTCC 2 cut(s) 906, 938
AlwI GGATC 4 cut(s) 122, 422, 602, 615
AlwNI CAGNNNCTG 1 cut(s) 1491
Aor13HI TCCGGA 1 cut(s) 1650
AoxI GGCC 1 cut(s) 917
ApeKI GCWGC 5 cut(s) 150, 1136, 1488, 1491, 1556
ApoI RAATTY 2 cut(s) 819, 1031
ArsI GACNNNNNNTTYG 2 cut(s) 929, 961
Asp700I GAANNNNTTC 1 cut(s) 70
Asp718I GGTACC 1 cut(s) 1579
AspLEI GCGC 5 cut(s) 150, 689, 1477, 1851, 1862
AspS9I GGNCC 1 cut(s) 1310
AsuHPI GGTGA 5 cut(s) 228, 1281, 1528, 1534, 1675
AvaII GGWCC 1 cut(s) 1310
AxyI CCTNAGG 3 cut(s) 633, 909, 1584
BaeGI GKGCMC 1 cut(s) 672
BamHI GGATCC 1 cut(s) 607
BanI GGYRCC 1 cut(s) 1579
BanII GRGCYC 2 cut(s) 1120, 1289
BauI CACGAG 2 cut(s) 198, 1185
BbvCI CCTCAGC 1 cut(s) 399
BbvI GCAGC 5 cut(s) 162, 1123, 1475, 1478, 1543
BccI CCATC 6 cut(s) 379, 439, 457, 475, 1034, 1958
BceAI ACGGC 1 cut(s) 932
BcgI CGANNNNNNTGC 2 cut(s) 1316, 1350
BciT130I CCWGG 2 cut(s) 145, 1836
BclI TGATCA 2 cut(s) 651, 1240
BcuI ACTAGT 1 cut(s) 1811
BfaI CTAG 1 cut(s) 1812
BfmI CTRYAG 1 cut(s) 1927
BfrI CTTAAG 1 cut(s) 1273
BisI GCNGC 7 cut(s) 151, 723, 1137, 1489, 1492, 1557, 1936
BlpI GCTNAGC 1 cut(s) 1919
BlsI GCNGC 7 cut(s) 152, 724, 1138, 1490, 1493, 1558, 1937
BmcAI AGTACT 2 cut(s) 191, 970
Bme1390I CCNGG 2 cut(s) 145, 1836
Bme18I GGWCC 1 cut(s) 1310
BmgT120I GGNCC 1 cut(s) 1310
BmiI GGNNCC 2 cut(s) 609, 1581
BmrFI CCNGG 2 cut(s) 145, 1836
BmrI ACTGGG 1 cut(s) 132
BmsI GCATC 4 cut(s) 17, 299, 1148, 1738
BmuI ACTGGG 1 cut(s) 132
BpmI CTGGAG 1 cut(s) 1425
Bpu10I CCTNAGC 1 cut(s) 399
Bpu1102I GCTNAGC 1 cut(s) 1919
BpuEI CTTGAG 1 cut(s) 1871
BsaBI GATNNNNATC 3 cut(s) 60, 1245, 1466
BsaHI GRCGYC 1 cut(s) 1146
BsaJI CCNNGG 1 cut(s) 143
BsaWI WCCGGW 2 cut(s) 74, 1650
BsaXI ACNNNNNCTCC 2 cut(s) 1398, 1428
Bsc4I CCNNNNNNNGG 3 cut(s) 144, 778, 986
Bse118I RCCGGY 1 cut(s) 322
Bse1I ACTGG 5 cut(s) 138, 956, 1312, 1408, 1831
Bse21I CCTNAGG 3 cut(s) 633, 909, 1584
Bse3DI GCAATG 2 cut(s) 764, 1866
Bse8I GATNNNNATC 3 cut(s) 60, 1245, 1466
BseAI TCCGGA 1 cut(s) 1650
BseBI CCWGG 2 cut(s) 145, 1836
BseDI CCNNGG 1 cut(s) 143
BseGI GGATG 6 cut(s) 380, 431, 805, 1601, 1753, 1754
BseJI GATNNNNATC 3 cut(s) 60, 1245, 1466
BseLI CCNNNNNNNGG 3 cut(s) 144, 778, 986
BseMI GCAATG 2 cut(s) 764, 1866
BseMII CTCAG 8 cut(s) 14, 200, 413, 624, 900, 1575, 1933, 1998
BseNI ACTGG 5 cut(s) 138, 956, 1312, 1408, 1831
BseRI GAGGAG 6 cut(s) 238, 511, 514, 517, 520, 523
BseSI GKGCMC 1 cut(s) 672
BseXI GCAGC 5 cut(s) 162, 1123, 1475, 1478, 1543
BseYI CCCAGC 2 cut(s) 511, 1851
BsgI GTGCAG 1 cut(s) 1188
Bsh1236I CGCG 4 cut(s) 687, 1020, 1849, 1860
BshFI GGCC 1 cut(s) 919
BshNI GGYRCC 1 cut(s) 1579
BsiSI CCGG 3 cut(s) 75, 323, 1651
BslFI GGGAC 3 cut(s) 126, 950, 1896
BslI CCNNNNNNNGG 3 cut(s) 144, 778, 986
BsmFI GGGAC 3 cut(s) 126, 950, 1896
BsmI GAATGC 2 cut(s) 1194, 1559
BsnI GGCC 1 cut(s) 919
Bsp1286I GDGCHC 3 cut(s) 672, 1120, 1289
Bsp13I TCCGGA 1 cut(s) 1650
Bsp143I GATC 6 cut(s) 16, 127, 427, 607, 651, 1240
Bsp1720I GCTNAGC 1 cut(s) 1919
BspACI CCGC 7 cut(s) 320, 723, 1259, 1597, 1758, 1847, 1936
BspANI GGCC 1 cut(s) 919
BspCNI CTCAG 8 cut(s) 15, 199, 412, 625, 901, 1576, 1932, 1997
BspEI TCCGGA 1 cut(s) 1650
BspFNI CGCG 4 cut(s) 687, 1020, 1849, 1860
BspHI TCATGA 2 cut(s) 624, 1680
BspLI GGNNCC 2 cut(s) 609, 1581
BspPI GGATC 4 cut(s) 122, 422, 602, 615
BspT107I GGYRCC 1 cut(s) 1579
BspTI CTTAAG 1 cut(s) 1273
BsrBI CCGCTC 2 cut(s) 725, 1599
BsrDI GCAATG 2 cut(s) 764, 1866
BsrFI RCCGGY 1 cut(s) 322
BsrI ACTGG 5 cut(s) 138, 956, 1312, 1408, 1831
BssAI RCCGGY 1 cut(s) 322
BssECI CCNNGG 1 cut(s) 143
BssMI GATC 6 cut(s) 16, 127, 427, 607, 651, 1240
BssNAI GTATAC 1 cut(s) 1429
BssNI GRCGYC 1 cut(s) 1146
BssSI CACGAG 2 cut(s) 198, 1185
Bst1107I GTATAC 1 cut(s) 1429
Bst2BI CACGAG 2 cut(s) 198, 1185
Bst2UI CCWGG 2 cut(s) 145, 1836
Bst4CI ACNGT 2 cut(s) 92, 1319
Bst6I CTCTTC 3 cut(s) 597, 1172, 1569
BstACI GRCGYC 1 cut(s) 1146
BstAFI CTTAAG 1 cut(s) 1273
BstC8I GCNNGC 1 cut(s) 1192
BstDEI CTNAG 9 cut(s) 23, 186, 387, 399, 633, 909, 1584, 1919, 1984
BstENI CCTNNNNNAGG 1 cut(s) 776
BstF5I GGATG 6 cut(s) 380, 431, 805, 1601, 1753, 1754
BstFNI CGCG 4 cut(s) 687, 1020, 1849, 1860
BstHHI GCGC 5 cut(s) 150, 689, 1477, 1851, 1862
BstKTI GATC 6 cut(s) 19, 130, 430, 610, 654, 1243
BstMBI GATC 6 cut(s) 16, 127, 427, 607, 651, 1240
BstMWI GCNNNNNNNGC 8 cut(s) 400, 1124, 1133, 1145, 1556, 1757, 1857, 1868
BstNI CCWGG 2 cut(s) 145, 1836
BstNSI RCATGY 1 cut(s) 1341
BstSCI CCNGG 2 cut(s) 143, 1834
BstSFI CTRYAG 1 cut(s) 1927
BstSLI GKGCMC 1 cut(s) 672
BstUI CGCG 4 cut(s) 687, 1020, 1849, 1860
BstV1I GCAGC 5 cut(s) 162, 1123, 1475, 1478, 1543
BstX2I RGATCY 2 cut(s) 427, 607
BstYI RGATCY 2 cut(s) 427, 607
BstZ17I GTATAC 1 cut(s) 1429
Bsu36I CCTNAGG 3 cut(s) 633, 909, 1584
BsuRI GGCC 1 cut(s) 919
BtgZI GCGATG 2 cut(s) 1509, 1936
BtsCI GGATG 6 cut(s) 380, 431, 805, 1601, 1753, 1754
BtsIMutI CAGTG 2 cut(s) 963, 1176
Cac8I GCNNGC 1 cut(s) 1192
CaiI CAGNNNCTG 1 cut(s) 1491
CciI TCATGA 2 cut(s) 624, 1680
CfoI GCGC 5 cut(s) 150, 689, 1477, 1851, 1862
Cfr10I RCCGGY 1 cut(s) 322
Cfr13I GGNCC 1 cut(s) 1310
CseI GACGC 1 cut(s) 1154
Csp6I GTAC 3 cut(s) 190, 969, 1580
CviAII CATG 6 cut(s) 6, 625, 1124, 1338, 1681, 1700
CviQI GTAC 3 cut(s) 190, 969, 1580
DdeI CTNAG 9 cut(s) 23, 186, 387, 399, 633, 909, 1584, 1919, 1984
DpnI GATC 6 cut(s) 18, 129, 429, 609, 653, 1242
DpnII GATC 6 cut(s) 16, 127, 427, 607, 651, 1240
EaeI YGGCCR 1 cut(s) 917
Eam1104I CTCTTC 3 cut(s) 597, 1172, 1569
EarI CTCTTC 3 cut(s) 597, 1172, 1569
Eco24I GRGCYC 2 cut(s) 1120, 1289
Eco32I GATATC 3 cut(s) 640, 1081, 1591
Eco47I GGWCC 1 cut(s) 1310
Eco57I CTGAAG 2 cut(s) 759, 1383
Eco81I CCTNAGG 3 cut(s) 633, 909, 1584
EcoNI CCTNNNNNAGG 1 cut(s) 776
EcoRII CCWGG 2 cut(s) 143, 1834
EcoRV GATATC 3 cut(s) 640, 1081, 1591
EcoT38I GRGCYC 2 cut(s) 1120, 1289
FaeI CATG 6 cut(s) 9, 628, 1127, 1341, 1684, 1703
FalI AAGNNNNNCTT 6 cut(s) 701, 733, 1336, 1368, 1378, 1410
FaqI GGGAC 3 cut(s) 126, 950, 1896
FatI CATG 6 cut(s) 5, 624, 1123, 1337, 1680, 1699
FbaI TGATCA 2 cut(s) 651, 1240
FblI GTMKAC 4 cut(s) 359, 492, 1428, 1535
Fnu4HI GCNGC 7 cut(s) 151, 723, 1137, 1489, 1492, 1557, 1936
FokI GGATG 6 cut(s) 367, 418, 812, 1588, 1741, 1760
FriOI GRGCYC 2 cut(s) 1120, 1289
Fsp4HI GCNGC 7 cut(s) 151, 723, 1137, 1489, 1492, 1557, 1936
FspBI CTAG 1 cut(s) 1812
GlaI GCGC 5 cut(s) 149, 688, 1476, 1850, 1861
GluI GCNGC 7 cut(s) 151, 723, 1137, 1489, 1492, 1557, 1936
GsaI CCCAGC 2 cut(s) 515, 1855
GsuI CTGGAG 1 cut(s) 1425
HaeIII GGCC 1 cut(s) 919
HapII CCGG 3 cut(s) 75, 323, 1651
HgaI GACGC 1 cut(s) 1154
HhaI GCGC 5 cut(s) 150, 689, 1477, 1851, 1862
Hin1I GRCGYC 1 cut(s) 1146
Hin1II CATG 6 cut(s) 9, 628, 1127, 1341, 1684, 1703
Hin6I GCGC 5 cut(s) 148, 687, 1475, 1849, 1860
HinP1I GCGC 5 cut(s) 148, 687, 1475, 1849, 1860
HincII GTYRAC 2 cut(s) 560, 1536
HindII GTYRAC 2 cut(s) 560, 1536
HindIII AAGCTT 2 cut(s) 1235, 1510
HpaII CCGG 3 cut(s) 75, 323, 1651
HphI GGTGA 5 cut(s) 228, 1281, 1528, 1534, 1675
Hpy166II GTNNAC 6 cut(s) 360, 493, 560, 1429, 1536, 1832
Hpy8I GTNNAC 6 cut(s) 360, 493, 560, 1429, 1536, 1832
HpyCH4III ACNGT 2 cut(s) 92, 1319
HpyCH4IV ACGT 1 cut(s) 1252
HpyCH4V TGCA 8 cut(s) 950, 1127, 1139, 1205, 1337, 1379, 1559, 1871
HpyF10VI GCNNNNNNNGC 8 cut(s) 400, 1124, 1133, 1145, 1556, 1757, 1857, 1868
HpyF3I CTNAG 9 cut(s) 23, 186, 387, 399, 633, 909, 1584, 1919, 1984
HpySE526I ACGT 1 cut(s) 1252
Hsp92I GRCGYC 1 cut(s) 1146
Hsp92II CATG 6 cut(s) 9, 628, 1127, 1341, 1684, 1703
HspAI GCGC 5 cut(s) 148, 687, 1475, 1849, 1860
Kpn2I TCCGGA 1 cut(s) 1650
KpnI GGTACC 1 cut(s) 1583
Ksp22I TGATCA 2 cut(s) 651, 1240
Kzo9I GATC 6 cut(s) 16, 127, 427, 607, 651, 1240
LmnI GCTCC 3 cut(s) 486, 754, 1011
Lsp1109I GCAGC 5 cut(s) 162, 1123, 1475, 1478, 1543
LweI GCATC 4 cut(s) 17, 299, 1148, 1738
MaeI CTAG 1 cut(s) 1812
MaeII ACGT 1 cut(s) 1252
MaeIII GTNAC 5 cut(s) 325, 550, 784, 1303, 1822
MalI GATC 6 cut(s) 18, 129, 429, 609, 653, 1242
MbiI CCGCTC 2 cut(s) 725, 1599
MboI GATC 6 cut(s) 16, 127, 427, 607, 651, 1240
MflI RGATCY 2 cut(s) 427, 607
MhlI GDGCHC 3 cut(s) 672, 1120, 1289
MluI ACGCGT 1 cut(s) 1018
MlyI GAGTC 5 cut(s) 1175, 1541, 1625, 1878, 1902
MmeI TCCRAC 4 cut(s) 777, 1089, 1243, 1699
MroI TCCGGA 1 cut(s) 1650
MroXI GAANNNNTTC 1 cut(s) 70
MseI TTAA 7 cut(s) 210, 366, 984, 1131, 1274, 1343, 1385
MspA1I CMGCKG 1 cut(s) 1488
MspCI CTTAAG 1 cut(s) 1273
MspI CCGG 3 cut(s) 75, 323, 1651
MspR9I CCNGG 2 cut(s) 145, 1836
Mva1269I GAATGC 2 cut(s) 1194, 1559
MvaI CCWGG 2 cut(s) 145, 1836
MvnI CGCG 4 cut(s) 687, 1020, 1849, 1860
MwoI GCNNNNNNNGC 8 cut(s) 400, 1124, 1133, 1145, 1556, 1757, 1857, 1868
NdeII GATC 6 cut(s) 16, 127, 427, 607, 651, 1240
NlaIII CATG 6 cut(s) 9, 628, 1127, 1341, 1684, 1703
NlaIV GGNNCC 2 cut(s) 609, 1581
NmuCI GTSAC 2 cut(s) 325, 784
NspI RCATGY 1 cut(s) 1341
PagI TCATGA 2 cut(s) 624, 1680
PctI GAATGC 2 cut(s) 1194, 1559
PdmI GAANNNNTTC 1 cut(s) 70
PfeI GAWTC 7 cut(s) 628, 682, 899, 1279, 1328, 1462, 1525
PflFI GACNNNGTC 1 cut(s) 941
PkrI GCNGC 7 cut(s) 152, 724, 1138, 1490, 1493, 1558, 1937
PleI GAGTC 5 cut(s) 1175, 1540, 1624, 1878, 1902
PpsI GAGTC 5 cut(s) 1175, 1540, 1624, 1878, 1902
PsiI TTATAA 4 cut(s) 255, 312, 816, 843
Psp1406I AACGTT 1 cut(s) 1252
Psp6I CCWGG 2 cut(s) 143, 1834
PspFI CCCAGC 2 cut(s) 511, 1851
PspGI CCWGG 2 cut(s) 143, 1834
PspN4I GGNNCC 2 cut(s) 609, 1581
PspPI GGNCC 1 cut(s) 1310
PstNI CAGNNNCTG 1 cut(s) 1491
PsuI RGATCY 2 cut(s) 427, 607
PsyI GACNNNGTC 1 cut(s) 941
PvuII CAGCTG 1 cut(s) 1488
RsaI GTAC 3 cut(s) 191, 970, 1581
RsaNI GTAC 3 cut(s) 190, 969, 1580
SalI GTCGAC 1 cut(s) 1534
SaqAI TTAA 7 cut(s) 210, 366, 984, 1131, 1274, 1343, 1385
SatI GCNGC 7 cut(s) 151, 723, 1137, 1489, 1492, 1557, 1936
Sau3AI GATC 6 cut(s) 16, 127, 427, 607, 651, 1240
Sau96I GGNCC 1 cut(s) 1310
ScaI AGTACT 2 cut(s) 191, 970
SchI GAGTC 5 cut(s) 1175, 1541, 1625, 1878, 1902
ScrFI CCNGG 2 cut(s) 145, 1836
SduI GDGCHC 3 cut(s) 672, 1120, 1289
SfaNI GCATC 4 cut(s) 17, 299, 1148, 1738
SfcI CTRYAG 1 cut(s) 1927
SinI GGWCC 1 cut(s) 1310
SmlI CTYRAG 2 cut(s) 1273, 1886
SmoI CTYRAG 2 cut(s) 1273, 1886
SpeI ACTAGT 1 cut(s) 1811
SsiI CCGC 7 cut(s) 320, 723, 1259, 1597, 1758, 1847, 1936
SspI AATATT 1 cut(s) 299
SspMI CTAG 1 cut(s) 1812
StyD4I CCNGG 2 cut(s) 143, 1834
TaaI ACNGT 2 cut(s) 92, 1319
TaiI ACGT 1 cut(s) 1255
TaqI TCGA 4 cut(s) 64, 1535, 1673, 1693
TatI WGTACW 2 cut(s) 189, 968
TauI GCSGC 2 cut(s) 725, 1938
TfiI GAWTC 7 cut(s) 628, 682, 899, 1279, 1328, 1462, 1525
Tru1I TTAA 7 cut(s) 210, 366, 984, 1131, 1274, 1343, 1385
Tru9I TTAA 7 cut(s) 210, 366, 984, 1131, 1274, 1343, 1385
TscAI CASTG 2 cut(s) 963, 1183
TseFI GTSAC 2 cut(s) 325, 784
TseI GCWGC 5 cut(s) 150, 1136, 1488, 1491, 1556
Tsp45I GTSAC 2 cut(s) 325, 784
TspGWI ACGGA 1 cut(s) 1553
TspRI CASTG 2 cut(s) 963, 1183
Tth111I GACNNNGTC 1 cut(s) 941
Vha464I CTTAAG 1 cut(s) 1273
VpaK11BI GGWCC 1 cut(s) 1310
XagI CCTNNNNNAGG 1 cut(s) 776
XapI RAATTY 2 cut(s) 819, 1031
XceI RCATGY 1 cut(s) 1341
XmiI GTMKAC 4 cut(s) 359, 492, 1428, 1535
XmnI GAANNNNTTC 1 cut(s) 70
XspI CTAG 1 cut(s) 1812
ZrmI AGTACT 2 cut(s) 191, 970
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.