Rh4CG463400

RNA helicase SDE3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0000595
Physical Location & Seq
Reverse (-)
69376449 .. 69384588
8140 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG463400.1

Sequence Viewer

Length: 2529 bp
ATGCAGTTACTGCATACTCGATCGTGTTGCAATGGTCTATCAGTGCTTTTGGAAAGAAGATGGAACCTTCAGCCTAATCTATATGACTCTCTCAATCTCTCAATTAATGGTCACTCACTGGCTCAACCATCTCAGGAGGACAATATGGATCAAGCACCACCTAAAGTGTCATCCCTTGAACCCCCAACATCTTCATCTAAACCACCCAAATCCTCTTTTGTTGATCCCAAACTGAAGCTACATTTGTCAGCTGCTTCCTCTCAGGGAAACAATCAAAAGGGGAAGGCAGTTCTGGTATGGACTGAAATGGTTTCATCTAAAGCACCACCTAAAGTGTCATCCCTTGAACCCCCAACATCTTCATCTAAACCACCCAAATCCTCTTTTGTTGATCCCAAACTGAAGCTACATTTGTCAGCTGCTTCCTCTCAGGGAAACAATCAAAAGGGGAAGGCAGTTCAGGTATGGACTGAAATGGTTTCATCTAAATCATCTGCATTCTCATCATTTTCTTCCAGGCCACCTCGATCTCCGTCTCCCCCTAAGTTCCCACAGTCCTTGGAACCAAGTCTTTCTGCTTCTTCCTCAAAGTCAAAGAGAAGAAACCGACAGAGAAAGAAAAAAAACCCGAGTGTTAAAAAGGGCTTATCTAGTCCATCCCCATCATCGTTAGAACCCCCATTGGTGATTTATTCTCCATCTTCCCCATCTTCATCAGAATCACCACCATCGTCCCCTAAGCTGCCTCCAGCCTTAAGGCAAACTTTTGCCTCAGCTCACTCGCATGTAAGAGATCAACGGGGGAAGACATGTGGTTATTCATTGATCAAAAGGGGCTTATCTAATCCATCTCCATCATCACTGAAACCCGCATTGTTGAGTTCTTTTGTACCGCCTCCATCTTGCCCTAGAGAGCATTCATCATCTTCATCTGGATCACCTCCATCATCCCCTAAGCTGCCTCCAACCTTAAGGCGAGCTTTCTCCTCACCTCACTCACATGTAGCCAATCAGCGGGGGAAGTCATATTATTCATGGGTGCAAAAGGGTTCATTACCTATATACAAGGTTCCCAAGAACATTAAAGTTTCGATCAAGAAAGATAGAGTGCCCAAAGTTCTTGACCAGCCATTGTCTCCCTTAACCTATAAGAACTACTTTGCTGCTCTCTTATATGCTGAGGAAGTCTACTTGGAGAATTGGAAAGATTATCAAATGTTGGAAGTGACATTGAACTTGCAAGAAGCAATTATAAAGGCTAAAAAGAAGGTTGAAAAAATCTTTGTAAAATTGAAGTTAGATTCAGTTCCTGAGAGACGCCCATTCCTTTTATCAAGGGATTTTGTCTATGCAAGACCTTCGGGTAGGGATGTTCAGCCATTTGAGGGCATTCTCTACCGTGTGGTGAAGAGCAGTCATATATTAGTTGATTTCGGACATGACTTTCTTTATCAGCATCATCCGAAACGCACATATGACATCAGCTTCTCATTTAACAGACTTTGTTTTAAAAGAGCTCATGCTGCAGTTCAAGCTGCATCATATCCTTTGTTCCAGAACTACATCTTTCCTGATTGTGTTCCAGAAAAGATCTTCATTTCCAATGATTCCACACCAAAGGCTGCAGTTAGACAGATTTTAGGCTTTTATGGCCCGCCACCCTATATTATAGAGGGCCAACGCTCCGTGATTCGTACAGGAAAAACTGAATCTAAACCGTATAAACTATCAAAAACAGGAGAGGTTGTTCGGGAAGCAGCATTGCAAATCTATAAAAGCTCTCCAGATGATCGTATTCTCATATGTGCCCCCTTAAACAGCACATGCGACGAGCTGATGATTAGTCTGAAGAAGGTGATTCCAGAGTGTGACATGTATCGAACCAATGCTGCATTTCGAGAGGTAGATGAGGTACCTATTGAGATTGTCCTCTCATGTGATCATGACGGCGAATGTTTTTCCTGTCCTCCACTCGCAGAACTCCAGAGGTTCAGGCTGATCTTCTCCACATTTGTTACTAGCTTTCGGCTACACAATGAAGGCGTCACTGCTGGACATTTCAGCCATATTTTTCTGGTGGATGCTGCTTTGGCTACTGAGCCAGAGACAATGATAGCTCTAGCTAATTTTGCTAATGAGAGCACAGCTGTTATAGTAACAGGTTCAGCCAATCATAAACCAAATCAGGTGCGCTCTGGTATGGCCAGGACCAAAGGATTGAAGACATCATACTTTGAAAGGCTCAGTGATGGCGTCCAAGGCTCCAAGAGAATCTTGAAGGAGCTCAAGGATCTCCAGAAAGATCCTCCTACCTCTTGTAGGGCTGGTCTCCATCTCTCTCACGAAATCTCCTCTAGCAATTGTAGTGGCTCATGTCTAAGTTTTCGGTTGGGCGATCGTAACTCTGCGGTAGGCGCTGGGCGGCTGGGCAGTCATGACAGTCGCTCTCCTTCGTCGCTCTCTCTCTGGCCCTCCTTGATGGGATCTCCCTCAGTCTCTCACCGCTGCATTACCGTGATGCCTTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

842

Amino Acids

92.84

Weight (kDa)

9.72

Isoelectric Point (pI)

60.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MOV-10_beta-barrel PF21634 417 - 496 9.7e-08 Helicase MOV-10, beta-barrel domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000595)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g37100 FvH4_4g37100 FvH4_4g37100
malus_domestica MD13G1238300.v1.1 MD16G1243100.v1.1 MD16G1243200.v1.1
prunus_persica Prupe.1G070100_v2.0.a1 Prupe.1G070200_v2.0.a1 Prupe.1G070300_v2.0.a1 Prupe.1G070300_v2.0.a1
pyrus_communis pycom13g21070 pycom16g20390 pycom16g20400
rosa_chinensis RchiOBHm_Chr1g0318071 RchiOBHm_Chr1g0318111 RchiOBHm_Chr4g0446861 RchiOBHm_Chr4g0446881 RchiOBHm_Chr4g0446891
rosa_laevigata RLG00000005623 RLG00000005624 RLG00000009337 RLG00000030560 RLG00000030566
rosa_multiflora Rmu_co8177450.1_g000001 Rmu_sc0001753.1_g000012 Rmu_sc0001753.1_g000013 Rmu_sc0001753.1_g000014 Rmu_sc0001753.1_g000015 Rmu_sc0011424.1_g000009 Rmu_sc0011424.1_g000020 Rmu_sc0034555.1_g000001 Rmu_ssc0000008.1_g000002
rosa_roxburghii Rroxscaffold_4G00329530 Rroxscaffold_4G00329590 Rroxscaffold_4G00329800 Rroxscaffold_4G00329810 Rroxscaffold_4G00329860 Rroxscaffold_4G00329910 Rroxscaffold_4G00330030 Rroxscaffold_5G00343610 Rroxscaffold_5G00348280 Rroxscaffold_5G00387220 Rroxscaffold_5G00387230
rosa_rugosa Rorug01G0019100 Rorug01G0019100 Rorug01G0019500 Rorug01G0019600 Rorug04G0010100 Rorug04G0375200 Rorug04G0375300 Rorug04G0375400 Rorug04G0375500 Rorug04G0375600 Rorug04G0375700
rosa_samantha Rh1AG030700 Rh1AG030900 Rh1BG026900 Rh1CG029500 Rh1CG029700 Rh1DG040500 Rh4AG088100 Rh4AG436700 Rh4AG436800 Rh4AG436900 Rh4BG084700 Rh4BG445800 Rh4BG445900 Rh4BG446100 Rh4CG096300 Rh4CG463100 Rh4CG463200 Rh4CG463400 Rh4DG444500 Rh4DG444600 Rh4DG444700
rosa_wichuraiana Rw1G002420 Rw1G002440 Rw1G002480 Rw4G037360 Rw4G037370 Rw4G037380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1253
Acc65I GGTACC 1 cut(s) 1912
AccB1I GGYRCC 1 cut(s) 1912
AccI GTMKAC 1 cut(s) 1188
AciI CCGC 7 cut(s) 870, 893, 1015, 1655, 2408, 2422, 2503
AclWI GGATC 7 cut(s) 156, 218, 386, 943, 2297, 2298, 2491
AcoI YGGCCR 1 cut(s) 2202
AcuI CTGAAG 4 cut(s) 53, 254, 422, 1868
AcyI GRCGYC 3 cut(s) 1318, 2043, 2253
AfaI GTAC 3 cut(s) 891, 1696, 1914
AfiI CCNNNNNNNGG 3 cut(s) 1014, 1385, 2319
AflII CTTAAG 2 cut(s) 754, 970
AflIII ACRYGT 3 cut(s) 809, 1000, 1872
AgsI TTSAA 9 cut(s) 179, 347, 1234, 1274, 1294, 1532, 2221, 2237, 2278
AjnI CCWGG 2 cut(s) 515, 2204
AloI GAACNNNNNNTCC 2 cut(s) 1731, 1763
Alw21I GWGCWC 3 cut(s) 1519, 2144, 2286
Alw26I GTCTC 6 cut(s) 540, 1140, 1309, 2099, 2333, 2500
AlwI GGATC 7 cut(s) 156, 218, 386, 943, 2297, 2298, 2491
AlwNI CAGNNNCTG 2 cut(s) 10, 1310
Ama87I CYCGRG 1 cut(s) 628
AoxI GGCC 5 cut(s) 518, 1651, 1675, 2202, 2468
AseI ATTAAT 1 cut(s) 105
Asp718I GGTACC 1 cut(s) 1912
AspLEI GCGC 2 cut(s) 2193, 2417
AspS9I GGNCC 4 cut(s) 1652, 1675, 2208, 2469
AsuHPI GGTGA 7 cut(s) 697, 714, 930, 981, 1417, 1867, 2492
AvaI CYCGRG 1 cut(s) 628
AvaII GGWCC 1 cut(s) 2208
BaeGI GKGCMC 2 cut(s) 1113, 1810
BalI TGGCCA 1 cut(s) 2204
BanI GGYRCC 1 cut(s) 1912
BanII GRGCYC 2 cut(s) 1519, 2286
BarI GAAGNNNNNNTAC 2 cut(s) 2213, 2245
BbsI GAAGAC 2 cut(s) 812, 2228
Bbv12I GWGCWC 3 cut(s) 1519, 2144, 2286
BbvCI CCTCAGC 2 cut(s) 772, 1179
BceAI ACGGC 1 cut(s) 1963
BcgI CGANNNNNNTGC 4 cut(s) 9, 43, 1341, 1375
BciT130I CCWGG 2 cut(s) 517, 2206
BclI TGATCA 2 cut(s) 825, 1939
BcoDI GTCTC 6 cut(s) 540, 1140, 1309, 2099, 2333, 2500
BfaI CTAG 5 cut(s) 651, 909, 2019, 2120, 2355
BfmI CTRYAG 2 cut(s) 1524, 1623
BfoI RGCGCY 1 cut(s) 2418
BfrI CTTAAG 2 cut(s) 754, 970
BglII AGATCT 1 cut(s) 1590
Bme1390I CCNGG 2 cut(s) 517, 2206
Bme18I GGWCC 1 cut(s) 2208
BmeT110I CYCGRG 1 cut(s) 628
BmgT120I GGNCC 4 cut(s) 1652, 1675, 2208, 2469
BmiI GGNNCC 5 cut(s) 65, 564, 1071, 1914, 2263
BmrFI CCNGG 2 cut(s) 517, 2206
BmsI GCATC 4 cut(s) 1465, 1547, 2071, 2508
BpiI GAAGAC 2 cut(s) 812, 2228
BpmI CTGGAG 4 cut(s) 732, 1767, 1967, 2279
Bpu10I CCTNAGC 4 cut(s) 738, 772, 954, 1179
BpuEI CTTGAG 1 cut(s) 2270
BsaHI GRCGYC 3 cut(s) 1318, 2043, 2253
BsaI GGTCTC 1 cut(s) 2333
BsaJI CCNNGG 2 cut(s) 558, 2257
BsaXI ACNNNNNCTCC 4 cut(s) 1731, 1761, 2333, 2363
Bsc4I CCNNNNNNNGG 3 cut(s) 1014, 1385, 2319
Bse1I ACTGG 1 cut(s) 123
Bse3DI GCAATG 2 cut(s) 37, 1760
BseBI CCWGG 2 cut(s) 517, 2206
BseDI CCNNGG 2 cut(s) 558, 2257
BseGI GGATG 7 cut(s) 170, 338, 656, 947, 1375, 1459, 2086
BseLI CCNNNNNNNGG 3 cut(s) 1014, 1385, 2319
BseMI GCAATG 2 cut(s) 37, 1760
BseMII CTCAG 9 cut(s) 146, 275, 443, 786, 1170, 1302, 2088, 2257, 2505
BseNI ACTGG 1 cut(s) 123
BseRI GAGGAG 2 cut(s) 976, 2341
BseSI GKGCMC 2 cut(s) 1113, 1810
BseYI CCCAGC 2 cut(s) 2417, 2425
Bsh1285I CGRYCG 2 cut(s) 23, 2398
BshFI GGCC 5 cut(s) 520, 1653, 1677, 2204, 2470
BshNI GGYRCC 1 cut(s) 1912
BsiEI CGRYCG 2 cut(s) 23, 2398
BsiHKAI GWGCWC 3 cut(s) 1519, 2144, 2286
BsiHKCI CYCGRG 1 cut(s) 628
BslFI GGGAC 1 cut(s) 718
BslI CCNNNNNNNGG 3 cut(s) 1014, 1385, 2319
BsmAI GTCTC 6 cut(s) 540, 1140, 1309, 2099, 2333, 2500
BsmBI CGTCTC 2 cut(s) 540, 1309
BsmFI GGGAC 1 cut(s) 718
BsmI GAATGC 3 cut(s) 497, 916, 1389
BsnI GGCC 5 cut(s) 520, 1653, 1677, 2204, 2470
Bso31I GGTCTC 1 cut(s) 2333
BsoBI CYCGRG 1 cut(s) 628
Bsp1286I GDGCHC 5 cut(s) 1113, 1519, 1810, 2144, 2286
BspACI CCGC 7 cut(s) 870, 893, 1015, 1655, 2408, 2422, 2503
BspANI GGCC 5 cut(s) 520, 1653, 1677, 2204, 2470
BspCNI CTCAG 9 cut(s) 145, 274, 442, 785, 1171, 1303, 2089, 2256, 2504
BspHI TCATGA 2 cut(s) 1942, 2434
BspLI GGNNCC 5 cut(s) 65, 564, 1071, 1914, 2263
BspMAI CTGCAG 2 cut(s) 1528, 1627
BspPI GGATC 7 cut(s) 156, 218, 386, 943, 2297, 2298, 2491
BspQI GCTCTTC 1 cut(s) 1403
BspT107I GGYRCC 1 cut(s) 1912
BspTI CTTAAG 2 cut(s) 754, 970
BspTNI GGTCTC 1 cut(s) 2333
BsrDI GCAATG 2 cut(s) 37, 1760
BsrI ACTGG 1 cut(s) 123
BssECI CCNNGG 2 cut(s) 558, 2257
BssNI GRCGYC 3 cut(s) 1318, 2043, 2253
BssT1I CCWWGG 2 cut(s) 558, 2257
Bst2UI CCWGG 2 cut(s) 517, 2206
Bst4CI ACNGT 5 cut(s) 555, 1400, 1719, 2441, 2515
Bst6I CTCTTC 1 cut(s) 1403
BstACI GRCGYC 3 cut(s) 1318, 2043, 2253
BstAFI CTTAAG 2 cut(s) 754, 970
BstAPI GCANNNNNTGC 1 cut(s) 10
BstC8I GCNNGC 2 cut(s) 978, 1655
BstENI CCTNNNNNAGG 1 cut(s) 2317
BstF5I GGATG 7 cut(s) 170, 338, 656, 947, 1375, 1459, 2086
BstH2I RGCGCY 1 cut(s) 2418
BstHHI GCGC 2 cut(s) 2193, 2417
BstMAI GTCTC 6 cut(s) 540, 1140, 1309, 2099, 2333, 2500
BstMCI CGRYCG 2 cut(s) 23, 2398
BstMWI GCNNNNNNNGC 8 cut(s) 10, 1523, 1532, 1650, 2090, 2129, 2259, 2414
BstNI CCWGG 2 cut(s) 517, 2206
BstNSI RCATGY 5 cut(s) 788, 813, 1004, 1827, 1876
BstSCI CCNGG 2 cut(s) 515, 2204
BstSFI CTRYAG 2 cut(s) 1524, 1623
BstSLI GKGCMC 2 cut(s) 1113, 1810
BstV2I GAAGAC 2 cut(s) 812, 2228
BstX2I RGATCY 4 cut(s) 1590, 2290, 2302, 2483
BstYI RGATCY 4 cut(s) 1590, 2290, 2302, 2483
BsuRI GGCC 5 cut(s) 520, 1653, 1677, 2204, 2470
BtsCI GGATG 7 cut(s) 170, 338, 656, 947, 1375, 1459, 2086
BtsI GCAGTG 1 cut(s) 2046
BtsIMutI CAGTG 5 cut(s) 48, 116, 860, 2046, 2251
Cac8I GCNNGC 2 cut(s) 978, 1655
CaiI CAGNNNCTG 2 cut(s) 10, 1310
CciI TCATGA 2 cut(s) 1942, 2434
CfoI GCGC 2 cut(s) 2193, 2417
Cfr13I GGNCC 4 cut(s) 1652, 1675, 2208, 2469
CseI GACGC 3 cut(s) 1326, 2032, 2242
Csp6I GTAC 3 cut(s) 890, 1695, 1913
CspCI CAANNNNNGTGG 4 cut(s) 540, 575, 2347, 2382
CviQI GTAC 3 cut(s) 890, 1695, 1913
DraI TTTAAA 1 cut(s) 1510
EaeI YGGCCR 1 cut(s) 2202
Eam1104I CTCTTC 1 cut(s) 1403
EarI CTCTTC 1 cut(s) 1403
Ecl136II GAGCTC 2 cut(s) 1517, 2284
Eco130I CCWWGG 2 cut(s) 558, 2257
Eco24I GRGCYC 2 cut(s) 1519, 2286
Eco31I GGTCTC 1 cut(s) 2333
Eco47I GGWCC 1 cut(s) 2208
Eco53kI GAGCTC 2 cut(s) 1517, 2284
Eco57I CTGAAG 4 cut(s) 53, 254, 422, 1868
Eco88I CYCGRG 1 cut(s) 628
EcoICRI GAGCTC 2 cut(s) 1517, 2284
EcoNI CCTNNNNNAGG 1 cut(s) 2317
EcoRII CCWGG 2 cut(s) 515, 2204
EcoT14I CCWWGG 2 cut(s) 558, 2257
EcoT38I GRGCYC 2 cut(s) 1519, 2286
ErhI CCWWGG 2 cut(s) 558, 2257
Esp3I CGTCTC 2 cut(s) 540, 1309
FalI AAGNNNNNCTT 8 cut(s) 748, 780, 964, 996, 1142, 1174, 2258, 2290
FaqI GGGAC 1 cut(s) 718
FauI CCCGC 3 cut(s) 877, 1008, 1662
FauNDI CATATG 2 cut(s) 1474, 1802
FbaI TGATCA 2 cut(s) 825, 1939
FblI GTMKAC 1 cut(s) 1188
FokI GGATG 7 cut(s) 157, 325, 643, 934, 1382, 1446, 2093
FriOI GRGCYC 2 cut(s) 1519, 2286
FspBI CTAG 5 cut(s) 651, 909, 2019, 2120, 2355
GlaI GCGC 2 cut(s) 2192, 2416
GsaI CCCAGC 2 cut(s) 2421, 2429
GsuI CTGGAG 4 cut(s) 732, 1767, 1967, 2279
HaeII RGCGCY 1 cut(s) 2418
HaeIII GGCC 5 cut(s) 520, 1653, 1677, 2204, 2470
HgaI GACGC 3 cut(s) 1326, 2032, 2242
HhaI GCGC 2 cut(s) 2193, 2417
Hin1I GRCGYC 3 cut(s) 1318, 2043, 2253
Hin6I GCGC 2 cut(s) 2191, 2415
HinP1I GCGC 2 cut(s) 2191, 2415
HinfI GANTC 8 cut(s) 86, 719, 1301, 1607, 1690, 1709, 1858, 2271
HphI GGTGA 7 cut(s) 697, 714, 930, 981, 1417, 1867, 2492
Hpy166II GTNNAC 1 cut(s) 1189
Hpy188I TCNGA 4 cut(s) 718, 1436, 1464, 1848
Hpy8I GTNNAC 1 cut(s) 1189
Hpy99I CGWCG 2 cut(s) 1832, 2458
HpyAV CCTTC 9 cut(s) 77, 277, 445, 1261, 1368, 1846, 2033, 2272, 2460
HpyCH4III ACNGT 5 cut(s) 555, 1400, 1719, 2441, 2515
HpyF10VI GCNNNNNNNGC 8 cut(s) 10, 1523, 1532, 1650, 2090, 2129, 2259, 2414
Hsp92I GRCGYC 3 cut(s) 1318, 2043, 2253
HspAI GCGC 2 cut(s) 2191, 2415
KpnI GGTACC 1 cut(s) 1916
Ksp22I TGATCA 2 cut(s) 825, 1939
LguI GCTCTTC 1 cut(s) 1403
LmnI GCTCC 3 cut(s) 1688, 2267, 2281
LweI GCATC 4 cut(s) 1465, 1547, 2071, 2508
MaeI CTAG 5 cut(s) 651, 909, 2019, 2120, 2355
MaeIII GTNAC 8 cut(s) 6, 110, 1225, 1868, 2014, 2044, 2155, 2399
MfeI CAATTG 1 cut(s) 2359
MflI RGATCY 4 cut(s) 1590, 2290, 2302, 2483
MhlI GDGCHC 5 cut(s) 1113, 1519, 1810, 2144, 2286
MlsI TGGCCA 1 cut(s) 2204
MluCI AATT 6 cut(s) 102, 1198, 1248, 1289, 2125, 2359
MluNI TGGCCA 1 cut(s) 2204
MlyI GAGTC 1 cut(s) 80
MmeI TCCRAC 2 cut(s) 989, 1200
Mox20I TGGCCA 1 cut(s) 2204
MscI TGGCCA 1 cut(s) 2204
MseI TTAA 9 cut(s) 105, 636, 755, 971, 1083, 1142, 1494, 1509, 1814
MslI CAYNNNNRTG 3 cut(s) 783, 999, 2513
Msp20I TGGCCA 1 cut(s) 2204
MspA1I CMGCKG 5 cut(s) 251, 419, 1015, 2147, 2505
MspCI CTTAAG 2 cut(s) 754, 970
MspR9I CCNGG 2 cut(s) 517, 2206
MunI CAATTG 1 cut(s) 2359
Mva1269I GAATGC 3 cut(s) 497, 916, 1389
MvaI CCWGG 2 cut(s) 517, 2206
MwoI GCNNNNNNNGC 8 cut(s) 10, 1523, 1532, 1650, 2090, 2129, 2259, 2414
NdeI CATATG 2 cut(s) 1474, 1802
NlaIV GGNNCC 5 cut(s) 65, 564, 1071, 1914, 2263
NmuCI GTSAC 4 cut(s) 110, 1225, 1868, 2044
NspI RCATGY 5 cut(s) 788, 813, 1004, 1827, 1876
PagI TCATGA 2 cut(s) 1942, 2434
PciI ACATGT 3 cut(s) 809, 1000, 1872
PciSI GCTCTTC 1 cut(s) 1403
PctI GAATGC 3 cut(s) 497, 916, 1389
PfeI GAWTC 7 cut(s) 719, 1301, 1607, 1690, 1709, 1858, 2271
Ple19I CGATCG 2 cut(s) 23, 2398
PleI GAGTC 1 cut(s) 80
PpsI GAGTC 1 cut(s) 80
PscI ACATGT 3 cut(s) 809, 1000, 1872
PshBI ATTAAT 1 cut(s) 105
PsiI TTATAA 1 cut(s) 1253
Psp124BI GAGCTC 2 cut(s) 1519, 2286
Psp6I CCWGG 2 cut(s) 515, 2204
PspFI CCCAGC 2 cut(s) 2417, 2425
PspGI CCWGG 2 cut(s) 515, 2204
PspN4I GGNNCC 5 cut(s) 65, 564, 1071, 1914, 2263
PspPI GGNCC 4 cut(s) 1652, 1675, 2208, 2469
PstI CTGCAG 2 cut(s) 1528, 1627
PstNI CAGNNNCTG 2 cut(s) 10, 1310
PsuI RGATCY 4 cut(s) 1590, 2290, 2302, 2483
PvuI CGATCG 2 cut(s) 23, 2398
PvuII CAGCTG 3 cut(s) 251, 419, 2147
RsaI GTAC 3 cut(s) 891, 1696, 1914
RsaNI GTAC 3 cut(s) 890, 1695, 1913
RseI CAYNNNNRTG 3 cut(s) 783, 999, 2513
SacI GAGCTC 2 cut(s) 1519, 2286
SapI GCTCTTC 1 cut(s) 1403
SaqAI TTAA 9 cut(s) 105, 636, 755, 971, 1083, 1142, 1494, 1509, 1814
Sau96I GGNCC 4 cut(s) 1652, 1675, 2208, 2469
SchI GAGTC 1 cut(s) 80
ScrFI CCNGG 2 cut(s) 517, 2206
SduI GDGCHC 5 cut(s) 1113, 1519, 1810, 2144, 2286
SfaNI GCATC 4 cut(s) 1465, 1547, 2071, 2508
SfcI CTRYAG 2 cut(s) 1524, 1623
SinI GGWCC 1 cut(s) 2208
SmiMI CAYNNNNRTG 3 cut(s) 783, 999, 2513
SmlI CTYRAG 3 cut(s) 754, 970, 2285
SmoI CTYRAG 3 cut(s) 754, 970, 2285
Sse9I AATT 6 cut(s) 102, 1198, 1248, 1289, 2125, 2359
SsiI CCGC 7 cut(s) 870, 893, 1015, 1655, 2408, 2422, 2503
SspMI CTAG 5 cut(s) 651, 909, 2019, 2120, 2355
SstI GAGCTC 2 cut(s) 1519, 2286
StyD4I CCNGG 2 cut(s) 515, 2204
StyI CCWWGG 2 cut(s) 558, 2257
TaaI ACNGT 5 cut(s) 555, 1400, 1719, 2441, 2515
TaqI TCGA 5 cut(s) 19, 526, 1091, 1879, 1897
TasI AATT 6 cut(s) 102, 1198, 1248, 1289, 2125, 2359
TauI GCSGC 1 cut(s) 2425
TfiI GAWTC 7 cut(s) 719, 1301, 1607, 1690, 1709, 1858, 2271
Tru1I TTAA 9 cut(s) 105, 636, 755, 971, 1083, 1142, 1494, 1509, 1814
Tru9I TTAA 9 cut(s) 105, 636, 755, 971, 1083, 1142, 1494, 1509, 1814
TscAI CASTG 5 cut(s) 48, 123, 867, 2053, 2251
TseFI GTSAC 4 cut(s) 110, 1225, 1868, 2044
Tsp45I GTSAC 4 cut(s) 110, 1225, 1868, 2044
TspGWI ACGGA 2 cut(s) 522, 1675
TspRI CASTG 5 cut(s) 48, 123, 867, 2053, 2251
Vha464I CTTAAG 2 cut(s) 754, 970
VpaK11BI GGWCC 1 cut(s) 2208
VspI ATTAAT 1 cut(s) 105
XagI CCTNNNNNAGG 1 cut(s) 2317
XceI RCATGY 5 cut(s) 788, 813, 1004, 1827, 1876
XmiI GTMKAC 1 cut(s) 1188
XspI CTAG 5 cut(s) 651, 909, 2019, 2120, 2355
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.