Rroxscaffold_4G00329530

RNA helicase SDE3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000595
Physical Location & Seq
Forward (+)
62846329 .. 62848767
2439 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00329530.1

Sequence Viewer

Length: 1611 bp
ATGATGAGGTTGGGGCTATCCATTGGGGCTTCAGCAATAGCAATATTATTGTGGGAATACTTTGAAAATGAGAGACCCCCACCATCATCATCCCCTGAAACGCCACCTCAGCGGCAGCCAACGTCATCTAATTCTAAACCACATCCAGCCTCCGCAGAACCCCCACCATATTATTCTAAACCATCTCCATCTTCCCCGCAACCCCCACCATATTCTTCTAAACCACCTTCATCTGCAGCTTCCCCACAACCCCCAGCATATTCTTCTAAACCATCTTCATCTCCATCTTCCCCAAAACACCCTCCAGCACATTTTTCTATACCATCTCCATCTCCATCTCCTTCTCCATCTTCATCTCCCCCAAAGTGCCCTCCTGTGTTTAAGTCAGTTCTACGCCCAGCCTCCTCTTCCTCAAATAATGATGATATAAGTCAACACCAACAATATGGGAAGAGAACAGCTTATGTGTGTGTTGAAGAGGATCCATTACCTGTATTCATGATTCCTGAGGATGTCGAAGCCTTGATCAAGAAAGAAATTGCGCCCAAAGTTCTGAATCGCCCATTATCTCCGACGACATACAAGGACTACTTTGCCGCTCTCTTATATTCTGAAGATTTCTACTTGGAGGGTGTGACATTAGCGTTGGATGAAAGTTCAATTTATAAAAACCGGAAAAAGGAAGGTAAGGAAGACAAAACCTTTGTAGTATTTGAGCTTGATCGTGTTCCTGAGGAACGGCCATTCCTCTTATCAAGGGACTTGGTCCTTGCACGACCAGTGGGTAATACTACTACTGAGCCCTTTAAGGGCCTCATCTATAAAGTTGTTGGGAGCAATCGCGTTTTGGTTGAATTTGAGGATGACTTTCATTCTTATCATCATTCCAACAAAAAATATGATATCAGCTTCTCATTCAATAGAGTTTGTTTGAAAAGAGCCCGCCATGCAGTAAAAGCTGCATCAGATGCCTTGTTTCAGAACTTCCTCTTCCCTGACTGTGACTCACGAGCAAGCATTCCGGCTGCACCGGCTCTGCATTCCTCTTATCATAAGCTTGATCAAGATCAAAGTTCTGCAGTCAGACATATCTTAAGGATTCAGGGCTCACCACCTTATGTAGTAACTGGTCCGGACTGTGAAGGCAAATCTTTTGCATATTTAAGAGAACCTTCAAGAACTGGAGTGGTTGTTAGTGAAGCAGTGATTCCAGAGTCAACTATGTTTCGTGCCAATGCTGCATTCCGAGAGAGAGATGAGGTACCTGAGGACATCCTCCGCTCATCCCTTTACAAAGAGTCTTGTTTTTCTTGTCCTGCAATAGAGAAACTCCGGGAATACAAGGTGATTTTCTCAACTTTCATGAGTAGCTTCCGACTACATGATAAAGGCATAGCTGCTGGACATTTTAGCCATATTTTTCTGGTGGATGCTTCATCTGCTATTGAGCCGGAAACACTGGTCGCCCTGACTAACTTTGCTGTCAAGGCCACTAGTGTTATAGTTACTGGTAATCATGGAAATAGTCCGCTCTGGGTTCGCGCTGACATTGCAAGGAAAAAGGAACTCAAGATTTCATACTTTGAACGACTCTGCAAGCTCAGGCCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

536

Amino Acids

59.51

Weight (kDa)

8.56

Isoelectric Point (pI)

68.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MOV-10_beta-barrel PF21634 229 - 304 6.2e-10 Helicase MOV-10, beta-barrel domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000595)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g37100 FvH4_4g37100 FvH4_4g37100
malus_domestica MD13G1238300.v1.1 MD16G1243100.v1.1 MD16G1243200.v1.1
prunus_persica Prupe.1G070100_v2.0.a1 Prupe.1G070200_v2.0.a1 Prupe.1G070300_v2.0.a1 Prupe.1G070300_v2.0.a1
pyrus_communis pycom13g21070 pycom16g20390 pycom16g20400
rosa_chinensis RchiOBHm_Chr1g0318071 RchiOBHm_Chr1g0318111 RchiOBHm_Chr4g0446861 RchiOBHm_Chr4g0446881 RchiOBHm_Chr4g0446891
rosa_laevigata RLG00000005623 RLG00000005624 RLG00000009337 RLG00000030560 RLG00000030566
rosa_multiflora Rmu_co8177450.1_g000001 Rmu_sc0001753.1_g000012 Rmu_sc0001753.1_g000013 Rmu_sc0001753.1_g000014 Rmu_sc0001753.1_g000015 Rmu_sc0011424.1_g000009 Rmu_sc0011424.1_g000020 Rmu_sc0034555.1_g000001 Rmu_ssc0000008.1_g000002
rosa_roxburghii Rroxscaffold_4G00329530 Rroxscaffold_4G00329590 Rroxscaffold_4G00329800 Rroxscaffold_4G00329810 Rroxscaffold_4G00329860 Rroxscaffold_4G00329910 Rroxscaffold_4G00330030 Rroxscaffold_5G00343610 Rroxscaffold_5G00348280 Rroxscaffold_5G00387220 Rroxscaffold_5G00387230
rosa_rugosa Rorug01G0019100 Rorug01G0019100 Rorug01G0019500 Rorug01G0019600 Rorug04G0010100 Rorug04G0375200 Rorug04G0375300 Rorug04G0375400 Rorug04G0375500 Rorug04G0375600 Rorug04G0375700
rosa_samantha Rh1AG030700 Rh1AG030900 Rh1BG026900 Rh1CG029500 Rh1CG029700 Rh1DG040500 Rh4AG088100 Rh4AG436700 Rh4AG436800 Rh4AG436900 Rh4BG084700 Rh4BG445800 Rh4BG445900 Rh4BG446100 Rh4CG096300 Rh4CG463100 Rh4CG463200 Rh4CG463400 Rh4DG444500 Rh4DG444600 Rh4DG444700
rosa_wichuraiana Rw1G002420 Rw1G002440 Rw1G002480 Rw4G037360 Rw4G037370 Rw4G037380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 666
Acc65I GGTACC 1 cut(s) 1261
AccB1I GGYRCC 1 cut(s) 1261
AccBSI CCGCTC 3 cut(s) 599, 1281, 1531
AccII CGCG 2 cut(s) 843, 1542
AccIII TCCGGA 1 cut(s) 1132
AciI CCGC 7 cut(s) 112, 153, 197, 597, 943, 1279, 1529
AclWI GGATC 2 cut(s) 476, 489
AcoI YGGCCR 1 cut(s) 740
AcsI RAATTY 1 cut(s) 854
AcuI CTGAAG 2 cut(s) 15, 633
AfaI GTAC 1 cut(s) 1263
AfiI CCNNNNNNNGG 2 cut(s) 679, 809
AflII CTTAAG 1 cut(s) 1093
AgsI TTSAA 8 cut(s) 65, 476, 660, 854, 919, 934, 1176, 1586
AhlI ACTAGT 1 cut(s) 1493
AjuI GAANNNNNNNTTGG 2 cut(s) 1226, 1258
AloI GAACNNNNNNTCC 2 cut(s) 729, 761
AluBI AGCT 9 cut(s) 239, 461, 718, 909, 959, 1057, 1371, 1397, 1600
AluI AGCT 9 cut(s) 239, 461, 718, 909, 959, 1057, 1371, 1397, 1600
Alw26I GTCTC 1 cut(s) 67
AlwI GGATC 2 cut(s) 476, 489
Aor13HI TCCGGA 1 cut(s) 1132
AoxI GGCC 4 cut(s) 740, 811, 1488, 1604
ApeKI GCWGC 6 cut(s) 115, 236, 959, 1025, 1238, 1397
ApoI RAATTY 1 cut(s) 854
ArsI GACNNNNNNTTYG 2 cut(s) 686, 718
Asp718I GGTACC 1 cut(s) 1261
AspLEI GCGC 2 cut(s) 544, 1544
AspS9I GGNCC 3 cut(s) 766, 811, 1130
AsuC2I CCSGG 1 cut(s) 1334
AsuHPI GGTGA 2 cut(s) 1101, 1357
AvaII GGWCC 2 cut(s) 766, 1130
AxyI CCTNAGG 3 cut(s) 507, 732, 1266
BaeGI GKGCMC 1 cut(s) 371
BamHI GGATCC 1 cut(s) 481
BanI GGYRCC 1 cut(s) 1261
BanII GRGCYC 3 cut(s) 804, 943, 1109
BauI CACGAG 1 cut(s) 1008
BbsI GAAGAC 1 cut(s) 699
BbvCI CCTCAGC 1 cut(s) 108
BbvI GCAGC 6 cut(s) 127, 248, 946, 1012, 1225, 1384
BccI CCATC 9 cut(s) 91, 190, 196, 280, 292, 331, 337, 343, 355
BceAI ACGGC 1 cut(s) 755
BclI TGATCA 2 cut(s) 525, 1060
BcnI CCSGG 1 cut(s) 1334
BcoDI GTCTC 1 cut(s) 67
BcuI ACTAGT 1 cut(s) 1493
BfaI CTAG 1 cut(s) 1494
BfmI CTRYAG 2 cut(s) 234, 1077
BfrI CTTAAG 1 cut(s) 1093
BisI GCNGC 8 cut(s) 113, 116, 237, 597, 960, 1026, 1239, 1398
BlsI GCNGC 8 cut(s) 114, 117, 238, 598, 961, 1027, 1240, 1399
Bme1390I CCNGG 1 cut(s) 1334
Bme18I GGWCC 2 cut(s) 766, 1130
BmgT120I GGNCC 3 cut(s) 766, 811, 1130
BmiI GGNNCC 2 cut(s) 483, 1263
BmrFI CCNGG 1 cut(s) 1334
BmsI GCATC 3 cut(s) 958, 971, 1420
BpiI GAAGAC 1 cut(s) 699
BpmI CTGGAG 2 cut(s) 288, 1203
Bpu10I CCTNAGC 2 cut(s) 108, 1601
BpuEI CTTGAG 1 cut(s) 1553
BpuMI CCSGG 1 cut(s) 1334
BsaBI GATNNNNATC 1 cut(s) 1065
BsaI GGTCTC 1 cut(s) 67
BsaWI WCCGGW 2 cut(s) 672, 1132
BsaXI ACNNNNNCTCC 2 cut(s) 1176, 1206
Bsc4I CCNNNNNNNGG 2 cut(s) 679, 809
Bse118I RCCGGY 1 cut(s) 1030
Bse1I ACTGG 5 cut(s) 779, 1132, 1186, 1464, 1513
Bse21I CCTNAGG 3 cut(s) 507, 732, 1266
Bse3DI GCAATG 1 cut(s) 1548
Bse8I GATNNNNATC 1 cut(s) 1065
BseAI TCCGGA 1 cut(s) 1132
BseGI GGATG 8 cut(s) 89, 142, 517, 655, 868, 1272, 1283, 1435
BseJI GATNNNNATC 1 cut(s) 1065
BseLI CCNNNNNNNGG 2 cut(s) 679, 809
BseMI GCAATG 1 cut(s) 1548
BseMII CTCAG 5 cut(s) 122, 498, 723, 789, 1257
BseNI ACTGG 5 cut(s) 779, 1132, 1186, 1464, 1513
BseRI GAGGAG 1 cut(s) 394
BseSI GKGCMC 1 cut(s) 371
BseXI GCAGC 6 cut(s) 127, 248, 946, 1012, 1225, 1384
BseYI CCCAGC 2 cut(s) 253, 397
BsgI GTGCAG 1 cut(s) 1011
Bsh1236I CGCG 2 cut(s) 843, 1542
BshFI GGCC 4 cut(s) 742, 813, 1490, 1606
BshNI GGYRCC 1 cut(s) 1261
BsiSI CCGG 6 cut(s) 673, 1022, 1031, 1133, 1333, 1451
BslFI GGGAC 1 cut(s) 773
BslI CCNNNNNNNGG 2 cut(s) 679, 809
BsmAI GTCTC 1 cut(s) 67
BsmFI GGGAC 1 cut(s) 773
BsmI GAATGC 3 cut(s) 1017, 1039, 1241
BsnI GGCC 4 cut(s) 742, 813, 1490, 1606
Bso31I GGTCTC 1 cut(s) 67
Bsp1286I GDGCHC 4 cut(s) 371, 804, 943, 1109
Bsp13I TCCGGA 1 cut(s) 1132
Bsp143I GATC 5 cut(s) 481, 525, 721, 1060, 1066
BspACI CCGC 7 cut(s) 112, 153, 197, 597, 943, 1279, 1529
BspANI GGCC 4 cut(s) 742, 813, 1490, 1606
BspCNI CTCAG 5 cut(s) 121, 499, 724, 790, 1258
BspEI TCCGGA 1 cut(s) 1132
BspFNI CGCG 2 cut(s) 843, 1542
BspHI TCATGA 2 cut(s) 498, 1362
BspLI GGNNCC 2 cut(s) 483, 1263
BspMAI CTGCAG 2 cut(s) 238, 1081
BspPI GGATC 2 cut(s) 476, 489
BspT107I GGYRCC 1 cut(s) 1261
BspTI CTTAAG 1 cut(s) 1093
BspTNI GGTCTC 1 cut(s) 67
BsrBI CCGCTC 3 cut(s) 599, 1281, 1531
BsrDI GCAATG 1 cut(s) 1548
BsrFI RCCGGY 1 cut(s) 1030
BsrI ACTGG 5 cut(s) 779, 1132, 1186, 1464, 1513
BssAI RCCGGY 1 cut(s) 1030
BssMI GATC 5 cut(s) 481, 525, 721, 1060, 1066
BssSI CACGAG 1 cut(s) 1008
Bst2BI CACGAG 1 cut(s) 1008
Bst4CI ACNGT 2 cut(s) 1001, 1139
Bst6I CTCTTC 4 cut(s) 412, 446, 471, 995
BstAFI CTTAAG 1 cut(s) 1093
BstAPI GCANNNNNTGC 1 cut(s) 968
BstC8I GCNNGC 3 cut(s) 943, 1015, 1598
BstDEI CTNAG 6 cut(s) 108, 507, 732, 798, 1266, 1601
BstF5I GGATG 8 cut(s) 89, 142, 517, 655, 868, 1272, 1283, 1435
BstFNI CGCG 2 cut(s) 843, 1542
BstHHI GCGC 2 cut(s) 544, 1544
BstKTI GATC 5 cut(s) 484, 528, 724, 1063, 1069
BstMAI GTCTC 1 cut(s) 67
BstMBI GATC 5 cut(s) 481, 525, 721, 1060, 1066
BstMWI GCNNNNNNNGC 9 cut(s) 109, 947, 956, 968, 1031, 1238, 1439, 1487, 1550
BstSCI CCNGG 1 cut(s) 1332
BstSFI CTRYAG 2 cut(s) 234, 1077
BstSLI GKGCMC 1 cut(s) 371
BstUI CGCG 2 cut(s) 843, 1542
BstV1I GCAGC 6 cut(s) 127, 248, 946, 1012, 1225, 1384
BstV2I GAAGAC 1 cut(s) 699
BstX2I RGATCY 1 cut(s) 481
BstXI CCANNNNNNTGG 1 cut(s) 446
BstYI RGATCY 1 cut(s) 481
Bsu36I CCTNAGG 3 cut(s) 507, 732, 1266
BsuRI GGCC 4 cut(s) 742, 813, 1490, 1606
BtsCI GGATG 8 cut(s) 89, 142, 517, 655, 868, 1272, 1283, 1435
BtsI GCAGTG 1 cut(s) 1209
BtsIMutI CAGTG 3 cut(s) 786, 1209, 1457
Cac8I GCNNGC 3 cut(s) 943, 1015, 1598
CciI TCATGA 2 cut(s) 498, 1362
CfoI GCGC 2 cut(s) 544, 1544
Cfr10I RCCGGY 1 cut(s) 1030
Cfr13I GGNCC 3 cut(s) 766, 811, 1130
Csp6I GTAC 1 cut(s) 1262
CviAII CATG 5 cut(s) 499, 947, 1363, 1382, 1517
CviQI GTAC 1 cut(s) 1262
DdeI CTNAG 6 cut(s) 108, 507, 732, 798, 1266, 1601
DpnI GATC 5 cut(s) 483, 527, 723, 1062, 1068
DpnII GATC 5 cut(s) 481, 525, 721, 1060, 1066
EaeI YGGCCR 1 cut(s) 740
Eam1104I CTCTTC 4 cut(s) 412, 446, 471, 995
EarI CTCTTC 4 cut(s) 412, 446, 471, 995
Eco24I GRGCYC 3 cut(s) 804, 943, 1109
Eco31I GGTCTC 1 cut(s) 67
Eco32I GATATC 1 cut(s) 904
Eco47I GGWCC 2 cut(s) 766, 1130
Eco57I CTGAAG 2 cut(s) 15, 633
Eco81I CCTNAGG 3 cut(s) 507, 732, 1266
EcoO109I RGGNCCY 1 cut(s) 811
EcoRV GATATC 1 cut(s) 904
EcoT38I GRGCYC 3 cut(s) 804, 943, 1109
FaeI CATG 5 cut(s) 502, 950, 1366, 1385, 1520
FalI AAGNNNNNCTT 4 cut(s) 575, 607, 1156, 1188
FaqI GGGAC 1 cut(s) 773
FatI CATG 5 cut(s) 498, 946, 1362, 1381, 1516
FauI CCCGC 2 cut(s) 204, 950
FbaI TGATCA 2 cut(s) 525, 1060
Fnu4HI GCNGC 8 cut(s) 113, 116, 237, 597, 960, 1026, 1239, 1398
FokI GGATG 8 cut(s) 76, 129, 524, 662, 875, 1259, 1270, 1442
FriOI GRGCYC 3 cut(s) 804, 943, 1109
Fsp4HI GCNGC 8 cut(s) 113, 116, 237, 597, 960, 1026, 1239, 1398
FspBI CTAG 1 cut(s) 1494
GlaI GCGC 2 cut(s) 543, 1543
GluI GCNGC 8 cut(s) 113, 116, 237, 597, 960, 1026, 1239, 1398
GsaI CCCAGC 2 cut(s) 257, 401
GsuI CTGGAG 2 cut(s) 288, 1203
HaeIII GGCC 4 cut(s) 742, 813, 1490, 1606
HapII CCGG 6 cut(s) 673, 1022, 1031, 1133, 1333, 1451
HhaI GCGC 2 cut(s) 544, 1544
Hin1II CATG 5 cut(s) 502, 950, 1366, 1385, 1520
Hin6I GCGC 2 cut(s) 542, 1542
HinP1I GCGC 2 cut(s) 542, 1542
HincII GTYRAC 2 cut(s) 434, 1218
HindII GTYRAC 2 cut(s) 434, 1218
HindIII AAGCTT 1 cut(s) 1055
HinfI GANTC 8 cut(s) 502, 556, 1004, 1099, 1207, 1214, 1298, 1590
HpaII CCGG 6 cut(s) 673, 1022, 1031, 1133, 1333, 1451
HphI GGTGA 2 cut(s) 1101, 1357
Hpy166II GTNNAC 2 cut(s) 434, 1218
Hpy188I TCNGA 8 cut(s) 555, 573, 613, 967, 981, 1085, 1247, 1376
Hpy8I GTNNAC 2 cut(s) 434, 1218
Hpy99I CGWCG 1 cut(s) 577
HpyAV CCTTC 5 cut(s) 237, 351, 677, 1136, 1182
HpyCH4III ACNGT 2 cut(s) 1001, 1139
HpyCH4IV ACGT 1 cut(s) 122
HpyF10VI GCNNNNNNNGC 9 cut(s) 109, 947, 956, 968, 1031, 1238, 1439, 1487, 1550
HpyF3I CTNAG 6 cut(s) 108, 507, 732, 798, 1266, 1601
HpySE526I ACGT 1 cut(s) 122
Hsp92II CATG 5 cut(s) 502, 950, 1366, 1385, 1520
HspAI GCGC 2 cut(s) 542, 1542
Kpn2I TCCGGA 1 cut(s) 1132
KpnI GGTACC 1 cut(s) 1265
Ksp22I TGATCA 2 cut(s) 525, 1060
Kzo9I GATC 5 cut(s) 481, 525, 721, 1060, 1066
LmnI GCTCC 1 cut(s) 834
Lsp1109I GCAGC 6 cut(s) 127, 248, 946, 1012, 1225, 1384
LweI GCATC 3 cut(s) 958, 971, 1420
MaeI CTAG 1 cut(s) 1494
MaeII ACGT 1 cut(s) 122
MaeIII GTNAC 4 cut(s) 634, 1001, 1123, 1504
MalI GATC 5 cut(s) 483, 527, 723, 1062, 1068
MbiI CCGCTC 3 cut(s) 599, 1281, 1531
MboI GATC 5 cut(s) 481, 525, 721, 1060, 1066
MflI RGATCY 1 cut(s) 481
MhlI GDGCHC 4 cut(s) 371, 804, 943, 1109
MluCI AATT 4 cut(s) 130, 537, 660, 854
MlyI GAGTC 4 cut(s) 998, 1223, 1307, 1584
MmeI TCCRAC 4 cut(s) 596, 627, 912, 1399
MroI TCCGGA 1 cut(s) 1132
MseI TTAA 4 cut(s) 381, 807, 1094, 1163
MspA1I CMGCKG 1 cut(s) 112
MspCI CTTAAG 1 cut(s) 1093
MspI CCGG 6 cut(s) 673, 1022, 1031, 1133, 1333, 1451
MspR9I CCNGG 1 cut(s) 1334
Mva1269I GAATGC 3 cut(s) 1017, 1039, 1241
MvnI CGCG 2 cut(s) 843, 1542
MwoI GCNNNNNNNGC 9 cut(s) 109, 947, 956, 968, 1031, 1238, 1439, 1487, 1550
NciI CCSGG 1 cut(s) 1334
NdeII GATC 5 cut(s) 481, 525, 721, 1060, 1066
NlaIII CATG 5 cut(s) 502, 950, 1366, 1385, 1520
NlaIV GGNNCC 2 cut(s) 483, 1263
NmuCI GTSAC 2 cut(s) 634, 1001
PagI TCATGA 2 cut(s) 498, 1362
PctI GAATGC 3 cut(s) 1017, 1039, 1241
PfeI GAWTC 4 cut(s) 502, 556, 1099, 1207
PflFI GACNNNGTC 1 cut(s) 764
PfoI TCCNGGA 1 cut(s) 1332
PkrI GCNGC 8 cut(s) 114, 117, 238, 598, 961, 1027, 1240, 1399
PleI GAGTC 4 cut(s) 998, 1222, 1306, 1584
PpsI GAGTC 4 cut(s) 998, 1222, 1306, 1584
PsiI TTATAA 1 cut(s) 666
PspFI CCCAGC 2 cut(s) 253, 397
PspN4I GGNNCC 2 cut(s) 483, 1263
PspPI GGNCC 3 cut(s) 766, 811, 1130
PstI CTGCAG 2 cut(s) 238, 1081
PsuI RGATCY 1 cut(s) 481
PsyI GACNNNGTC 1 cut(s) 764
RsaI GTAC 1 cut(s) 1263
RsaNI GTAC 1 cut(s) 1262
SaqAI TTAA 4 cut(s) 381, 807, 1094, 1163
SatI GCNGC 8 cut(s) 113, 116, 237, 597, 960, 1026, 1239, 1398
Sau3AI GATC 5 cut(s) 481, 525, 721, 1060, 1066
Sau96I GGNCC 3 cut(s) 766, 811, 1130
SchI GAGTC 4 cut(s) 998, 1223, 1307, 1584
ScrFI CCNGG 1 cut(s) 1334
SduI GDGCHC 4 cut(s) 371, 804, 943, 1109
SfaNI GCATC 3 cut(s) 958, 971, 1420
SfcI CTRYAG 2 cut(s) 234, 1077
SinI GGWCC 2 cut(s) 766, 1130
SmlI CTYRAG 2 cut(s) 1093, 1568
SmoI CTYRAG 2 cut(s) 1093, 1568
SpeI ACTAGT 1 cut(s) 1493
Sse9I AATT 4 cut(s) 130, 537, 660, 854
SsiI CCGC 7 cut(s) 112, 153, 197, 597, 943, 1279, 1529
SspI AATATT 1 cut(s) 45
SspMI CTAG 1 cut(s) 1494
StyD4I CCNGG 1 cut(s) 1332
TaaI ACNGT 2 cut(s) 1001, 1139
TaiI ACGT 1 cut(s) 125
TaqI TCGA 1 cut(s) 516
TasI AATT 4 cut(s) 130, 537, 660, 854
TauI GCSGC 2 cut(s) 115, 599
TfiI GAWTC 4 cut(s) 502, 556, 1099, 1207
Tru1I TTAA 4 cut(s) 381, 807, 1094, 1163
Tru9I TTAA 4 cut(s) 381, 807, 1094, 1163
TscAI CASTG 3 cut(s) 786, 1209, 1464
TseFI GTSAC 2 cut(s) 634, 1001
TseI GCWGC 6 cut(s) 115, 236, 959, 1025, 1238, 1397
Tsp45I GTSAC 2 cut(s) 634, 1001
TspDTI ATGAA 9 cut(s) 219, 267, 342, 487, 666, 860, 1351, 1425, 1566
TspRI CASTG 3 cut(s) 786, 1209, 1464
Tth111I GACNNNGTC 1 cut(s) 764
Vha464I CTTAAG 1 cut(s) 1093
VpaK11BI GGWCC 2 cut(s) 766, 1130
XapI RAATTY 1 cut(s) 854
XspI CTAG 1 cut(s) 1494
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.