Rroxscaffold_4G00329590

RNA helicase SDE3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000595
Physical Location & Seq
Forward (+)
62875709 .. 62882221
6513 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00329590.1

Sequence Viewer

Length: 2616 bp
ATGCACCCCCTTCAACAAAGCACATGCGACGAGTTGACGATTAGTTTGAAGAACATGATTCCAGATCGTGACATTTATCAAACCAATGCTGCATTTCGAGAGGTAGATGAGGTACCTATTGCAATTCCCTCTTCATGTGATCATGACGGCGAGTGTGATCGGAATTTGCCTCGCCGTTCTAAACTAGGGCGTGCTGCTGGAAAAGCGTACGAAAAAGCATTGGGACTGCCATTTTTGCCGAAGGCGAGGTTTGATGCAGCGGGCGGTGGAAGGAAGAAGATGAAGTTGCGTCTGTCACCCCACCGCCTTGAGGCACTCGCCTTGCGAGGCACTAATAAGACGCCTTCTCCTTCACTTTCGCATTTGAAAACATTGATGAGAGCGCAGCTGTTGGGGCTATCCATTGGGGCTTCAGCAATAGCAATATTATTGTGGGAATACTTTGAAAATGAGAGACCCCCACCATCATTATCCCCTGAAACGCCACCTCAGCGGCAGCCAACGTCATCTAATTCTAAACCACATCCAGCCTCCGCAGAACCCCCACCATATTATTCTAAACCATCTCCATCTCCATCTTCCCCGAAACCCCCACCATATTCTTCTAAACCACCTTCATCTCCAGCTTCTTCTAAACCACCTTCATCCCCAGTTTCTTCTAAACCACCTGCATCCCCAACTTCTTCTAAACCACCTTCATTCCCAGCTTCTTCTCCCCCACCATATTCTTCTAAACCACCTTCATCCCCAGCTTCTTCTAAACCACCTTCATCCCCAGCTTCTTCTTCCCCACCATATTCTTCTAAACCACCTTCATCCCCAGCTTCTTCTAAACCACCTTCATCTGCAGCTTCCCCACAACCCCCAGCATATTCTTCTAAACCACCTGCATCCCCAGCATGTTCTTCTAAACCACCTTCATCTCCAGTTTCCCCACAACCCCCAGCATATTCTTCTAAACCATCTTCATCTCCATCTTCCCCAAAACACCCTCCAGCACATTTTTCTATACCATCTCCATCTCCACCTCCATCTCCCCCAAAGTGCCCTCCTGTCTTTAAGTCAGTTCTACGCCCAGCCTCCTCTTCCTCAAATAATGATGATATAAGTCAAAACCAACAATATGGGAAGAGAACAGCTTATGTGTGTGTTGAAGAGGATCCATTACCTGTATTCATGATTCCTGAGGATGTCGAAGCCTTGATCAAGAAAGAAATTGCGCCCAAAGTTCTGAATCGCCCATTATCTCCCACAACATACAAGGACTACTTTGCCGCTCTCTTATATTCTGAAGATTTCTACTTGGAGAAATGGAGTGACTTCCTTTTGAAGGGTGTGACATTAGCGTTGGATGAAAGTTCAATTTATAAAAACCGGAAAAAGGAAGCACTTTATAAAAATCGGAAAAAGGAAGATAAGGAAGACAAAACCTTTGTAGCATTTGAGCTTGATTGTGTTCCTGAGAAACGGCCATTCCTCTTATCAAGGGACTTGGTCCTTGCACGACCAGTGGGTAATAGTACTACTGAGCCCTTTAAGGGCCCCATCCATAAAGTTGTTGGGAGCAATCGCGTTTTGGTTGAATTTGAGGATGGCTTTTATTCTTATCATCATTCCAACAAAAAATATGATATCAGCTTCTCATACAATAGAGTTTGTTTGAAAAGAGCCCACCGTGCAGTAAAAGCTGCATCAGATGCCTTGTTTCAGAACTTCCTCTTCCCTGACTGTGACTCACGAGCAAGCATTCCTGCTGCACCAGCTCTGCTTTCTACTACCTATCATAAGCTTGATCAAGAGCAACGTTCTGCAGTCGGACATATCTTAAGGATTCAGGGCTCACCACCTTATGTAGTAACTGGTCCAGACTGTGAAGGCAAATCTTTTGCAAATTTAAGAGAACCTTCAAGAACTGGAGTGGTTGTTAGTGAAGCAGTATACCAATTATGTCGGAAGTCACCAGAGTATCGCATTCTTATCTGTGCGCCTACTAACAGCTGCTGCGATATGCTGATGAGAAGCTTGGTGAAGGTGATTCCAGAGTCTACTATGTTTCGTGCCAATGCAGCATTCCGAGACAGAGATGAGGTACCTGAGGACATCCTACGTTCATCCCTTTACAAAGAGTCTTGTTTTTCTTGTCCTGCAATAGAGAAACTCCGGGAATACAAGGTGATTTTCTCAACTTTCATGAGTAGCTTCCGACTACATGATAAAGGCATAGCTGCTGGACATTTTAGCCATATTTTTCTGGTGGATGCTTCATCTGCTATTGAGCCGGAAACATTGGTCGCCCTGACTAACTTTGCTGTCAAGGCCACTAGTGTTATAGTTACTGGTAATCATGGAAATAGTCCGCTCTGGGTTCGTGCTGACATTGCAAGGAAAAAGGGACTCAAGATTTCATACTTTGAACGACTCTGCAAGCTCAGGCCATATAGAAGCCCCAGTCCAATGTTCATCACACAACTAGACCTGAACCAAAAGTTGCATGGATTCCTAGAAGATGTCATGATTCCAGACCGGCAAGATCCCACAGTCCCCAACTTGGATGGAAACAACTATGAACTTCCAAACAAATGCAAAAGGTGTCAACGTATCTTTGGTAGCGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

871

Amino Acids

95.64

Weight (kDa)

9.31

Isoelectric Point (pI)

71.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MOV-10_beta-barrel PF21634 471 - 547 1.8e-09 Helicase MOV-10, beta-barrel domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000595)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g37100 FvH4_4g37100 FvH4_4g37100
malus_domestica MD13G1238300.v1.1 MD16G1243100.v1.1 MD16G1243200.v1.1
prunus_persica Prupe.1G070100_v2.0.a1 Prupe.1G070200_v2.0.a1 Prupe.1G070300_v2.0.a1 Prupe.1G070300_v2.0.a1
pyrus_communis pycom13g21070 pycom16g20390 pycom16g20400
rosa_chinensis RchiOBHm_Chr1g0318071 RchiOBHm_Chr1g0318111 RchiOBHm_Chr4g0446861 RchiOBHm_Chr4g0446881 RchiOBHm_Chr4g0446891
rosa_laevigata RLG00000005623 RLG00000005624 RLG00000009337 RLG00000030560 RLG00000030566
rosa_multiflora Rmu_co8177450.1_g000001 Rmu_sc0001753.1_g000012 Rmu_sc0001753.1_g000013 Rmu_sc0001753.1_g000014 Rmu_sc0001753.1_g000015 Rmu_sc0011424.1_g000009 Rmu_sc0011424.1_g000020 Rmu_sc0034555.1_g000001 Rmu_ssc0000008.1_g000002
rosa_roxburghii Rroxscaffold_4G00329530 Rroxscaffold_4G00329590 Rroxscaffold_4G00329800 Rroxscaffold_4G00329810 Rroxscaffold_4G00329860 Rroxscaffold_4G00329910 Rroxscaffold_4G00330030 Rroxscaffold_5G00343610 Rroxscaffold_5G00348280 Rroxscaffold_5G00387220 Rroxscaffold_5G00387230
rosa_rugosa Rorug01G0019100 Rorug01G0019100 Rorug01G0019500 Rorug01G0019600 Rorug04G0010100 Rorug04G0375200 Rorug04G0375300 Rorug04G0375400 Rorug04G0375500 Rorug04G0375600 Rorug04G0375700
rosa_samantha Rh1AG030700 Rh1AG030900 Rh1BG026900 Rh1CG029500 Rh1CG029700 Rh1DG040500 Rh4AG088100 Rh4AG436700 Rh4AG436800 Rh4AG436900 Rh4BG084700 Rh4BG445800 Rh4BG445900 Rh4BG446100 Rh4CG096300 Rh4CG463100 Rh4CG463200 Rh4CG463400 Rh4DG444500 Rh4DG444600 Rh4DG444700
rosa_wichuraiana Rw1G002420 Rw1G002440 Rw1G002480 Rw4G037360 Rw4G037370 Rw4G037380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1368, 1395
AarI CACCTGC 2 cut(s) 676, 895
Acc36I ACCTGC 2 cut(s) 676, 895
Acc65I GGTACC 2 cut(s) 112, 2089
AccB1I GGYRCC 2 cut(s) 112, 2089
AccBSI CCGCTC 2 cut(s) 1277, 2359
AccI GTMKAC 2 cut(s) 1938, 2045
AccII CGCG 1 cut(s) 1572
AciI CCGC 7 cut(s) 260, 264, 304, 493, 534, 1275, 2357
AclI AACGTT 1 cut(s) 1804
AclWI GGATC 3 cut(s) 1154, 1167, 2525
AcoI YGGCCR 1 cut(s) 1469
AcsI RAATTY 3 cut(s) 163, 1583, 1891
AcuI CTGAAG 2 cut(s) 396, 1311
AcyI GRCGYC 1 cut(s) 341
AfaI GTAC 4 cut(s) 114, 209, 1522, 2091
AfiI CCNNNNNNNGG 5 cut(s) 310, 1330, 1381, 1538, 2548
AflII CTTAAG 1 cut(s) 1825
AhlI ACTAGT 1 cut(s) 2321
AjuI GAANNNNNNNTTGG 2 cut(s) 2054, 2086
Alw26I GTCTC 2 cut(s) 448, 2070
AlwI GGATC 3 cut(s) 1154, 1167, 2525
AlwNI CAGNNNCTG 1 cut(s) 2001
AoxI GGCC 4 cut(s) 1469, 1540, 2316, 2432
ApaI GGGCCC 1 cut(s) 1544
ApoI RAATTY 3 cut(s) 163, 1583, 1891
ArsI GACNNNNNNTTYG 4 cut(s) 28, 60, 1415, 1447
Asp718I GGTACC 2 cut(s) 112, 2089
AspLEI GCGC 3 cut(s) 385, 1222, 1987
AspS9I GGNCC 4 cut(s) 1495, 1540, 1541, 1862
AsuC2I CCSGG 1 cut(s) 2162
AsuHPI GGTGA 6 cut(s) 288, 1833, 1950, 2038, 2044, 2185
AvaII GGWCC 2 cut(s) 1495, 1862
AxyI CCTNAGG 2 cut(s) 1185, 2094
BaeGI GKGCMC 2 cut(s) 1049, 1544
BaeI ACNNNNGTAYC 2 cut(s) 1949, 1982
BamHI GGATCC 1 cut(s) 1159
BanI GGYRCC 2 cut(s) 112, 2089
BanII GRGCYC 4 cut(s) 1533, 1544, 1672, 1841
BauI CACGAG 1 cut(s) 1737
BbsI GAAGAC 1 cut(s) 1428
BbvCI CCTCAGC 1 cut(s) 489
BceAI ACGGC 3 cut(s) 159, 163, 1484
BclI TGATCA 3 cut(s) 139, 1203, 1792
BcnI CCSGG 1 cut(s) 2162
BcoDI GTCTC 2 cut(s) 448, 2070
BcuI ACTAGT 1 cut(s) 2321
BfaI CTAG 4 cut(s) 185, 2322, 2471, 2501
BfmI CTRYAG 2 cut(s) 846, 1809
BfrI CTTAAG 1 cut(s) 1825
BfuAI ACCTGC 2 cut(s) 676, 895
BmcAI AGTACT 1 cut(s) 1522
Bme1390I CCNGG 1 cut(s) 2162
Bme18I GGWCC 2 cut(s) 1495, 1862
BmgT120I GGNCC 4 cut(s) 1495, 1540, 1541, 1862
BmiI GGNNCC 5 cut(s) 114, 1161, 1542, 1543, 2091
BmrFI CCNGG 1 cut(s) 2162
BmrI ACTGGG 2 cut(s) 644, 2442
BmsI GCATC 6 cut(s) 244, 680, 899, 1687, 1700, 2248
BmuI ACTGGG 2 cut(s) 644, 2442
BpiI GAAGAC 1 cut(s) 1428
BpmI CTGGAG 4 cut(s) 606, 909, 978, 1935
Bpu10I CCTNAGC 2 cut(s) 489, 2429
BpuEI CTTGAG 2 cut(s) 329, 2381
BpuMI CCSGG 1 cut(s) 2162
BsaHI GRCGYC 1 cut(s) 341
BsaI GGTCTC 1 cut(s) 448
BsaWI WCCGGW 1 cut(s) 1374
BsaXI ACNNNNNCTCC 4 cut(s) 331, 361, 1908, 1938
Bsc4I CCNNNNNNNGG 5 cut(s) 310, 1330, 1381, 1538, 2548
Bse118I RCCGGY 1 cut(s) 2523
Bse1I ACTGG 7 cut(s) 650, 926, 1508, 1864, 1918, 2341, 2448
Bse21I CCTNAGG 2 cut(s) 1185, 2094
Bse3DI GCAATG 1 cut(s) 2376
BseLI CCNNNNNNNGG 5 cut(s) 310, 1330, 1381, 1538, 2548
BseMI GCAATG 1 cut(s) 2376
BseMII CTCAG 6 cut(s) 503, 1176, 1452, 1518, 2085, 2443
BseNI ACTGG 7 cut(s) 650, 926, 1508, 1864, 1918, 2341, 2448
BseRI GAGGAG 1 cut(s) 1072
BseSI GKGCMC 2 cut(s) 1049, 1544
BseYI CCCAGC 8 cut(s) 703, 748, 775, 820, 865, 895, 943, 1075
BsgI GTGCAG 2 cut(s) 1698, 1740
Bsh1236I CGCG 1 cut(s) 1572
BshFI GGCC 4 cut(s) 1471, 1542, 2318, 2434
BshNI GGYRCC 2 cut(s) 112, 2089
BsiSI CCGG 4 cut(s) 1375, 2161, 2279, 2524
BsiWI CGTACG 1 cut(s) 207
BslFI GGGAC 4 cut(s) 237, 1502, 2406, 2525
BslI CCNNNNNNNGG 5 cut(s) 310, 1330, 1381, 1538, 2548
BsmAI GTCTC 2 cut(s) 448, 2070
BsmFI GGGAC 4 cut(s) 237, 1502, 2406, 2525
BsmI GAATGC 3 cut(s) 1746, 1971, 2069
BsnI GGCC 4 cut(s) 1471, 1542, 2318, 2434
Bso31I GGTCTC 1 cut(s) 448
Bsp120I GGGCCC 1 cut(s) 1540
Bsp1286I GDGCHC 5 cut(s) 1049, 1533, 1544, 1672, 1841
Bsp143I GATC 7 cut(s) 64, 139, 157, 1159, 1203, 1792, 2530
BspACI CCGC 7 cut(s) 260, 264, 304, 493, 534, 1275, 2357
BspANI GGCC 4 cut(s) 1471, 1542, 2318, 2434
BspCNI CTCAG 6 cut(s) 502, 1177, 1453, 1519, 2086, 2442
BspFNI CGCG 1 cut(s) 1572
BspHI TCATGA 4 cut(s) 142, 1176, 2190, 2511
BspLI GGNNCC 5 cut(s) 114, 1161, 1542, 1543, 2091
BspMAI CTGCAG 2 cut(s) 850, 1813
BspMI ACCTGC 2 cut(s) 676, 895
BspPI GGATC 3 cut(s) 1154, 1167, 2525
BspT107I GGYRCC 2 cut(s) 112, 2089
BspTI CTTAAG 1 cut(s) 1825
BspTNI GGTCTC 1 cut(s) 448
BsrBI CCGCTC 2 cut(s) 1277, 2359
BsrDI GCAATG 1 cut(s) 2376
BsrFI RCCGGY 1 cut(s) 2523
BsrI ACTGG 7 cut(s) 650, 926, 1508, 1864, 1918, 2341, 2448
BssAI RCCGGY 1 cut(s) 2523
BssMI GATC 7 cut(s) 64, 139, 157, 1159, 1203, 1792, 2530
BssNAI GTATAC 1 cut(s) 1939
BssNI GRCGYC 1 cut(s) 341
BssSI CACGAG 1 cut(s) 1737
Bst1107I GTATAC 1 cut(s) 1939
Bst2BI CACGAG 1 cut(s) 1737
Bst4CI ACNGT 4 cut(s) 1676, 1730, 1871, 2539
Bst6I CTCTTC 5 cut(s) 136, 1090, 1124, 1149, 1724
BstACI GRCGYC 1 cut(s) 341
BstAFI CTTAAG 1 cut(s) 1825
BstAPI GCANNNNNTGC 1 cut(s) 1697
BstC8I GCNNGC 4 cut(s) 192, 262, 1744, 2426
BstDEI CTNAG 6 cut(s) 489, 1185, 1461, 1527, 2094, 2429
BstENI CCTNNNNNAGG 1 cut(s) 1328
BstFNI CGCG 1 cut(s) 1572
BstHHI GCGC 3 cut(s) 385, 1222, 1987
BstKTI GATC 7 cut(s) 67, 142, 160, 1162, 1206, 1795, 2533
BstMAI GTCTC 2 cut(s) 448, 2070
BstMBI GATC 7 cut(s) 64, 139, 157, 1159, 1203, 1792, 2530
BstNSI RCATGY 2 cut(s) 27, 903
BstSCI CCNGG 1 cut(s) 2160
BstSFI CTRYAG 2 cut(s) 846, 1809
BstSLI GKGCMC 2 cut(s) 1049, 1544
BstUI CGCG 1 cut(s) 1572
BstV2I GAAGAC 1 cut(s) 1428
BstX2I RGATCY 2 cut(s) 1159, 2530
BstXI CCANNNNNNTGG 1 cut(s) 1124
BstYI RGATCY 2 cut(s) 1159, 2530
BstZ17I GTATAC 1 cut(s) 1939
Bsu36I CCTNAGG 2 cut(s) 1185, 2094
BsuRI GGCC 4 cut(s) 1471, 1542, 2318, 2434
BtsIMutI CAGTG 1 cut(s) 1515
BveI ACCTGC 2 cut(s) 676, 895
Cac8I GCNNGC 4 cut(s) 192, 262, 1744, 2426
CaiI CAGNNNCTG 1 cut(s) 2001
CciI TCATGA 4 cut(s) 142, 1176, 2190, 2511
CfoI GCGC 3 cut(s) 385, 1222, 1987
Cfr10I RCCGGY 1 cut(s) 2523
Cfr13I GGNCC 4 cut(s) 1495, 1540, 1541, 1862
CseI GACGC 2 cut(s) 278, 349
Csp6I GTAC 4 cut(s) 113, 208, 1521, 2090
CviQI GTAC 4 cut(s) 113, 208, 1521, 2090
DdeI CTNAG 6 cut(s) 489, 1185, 1461, 1527, 2094, 2429
DpnI GATC 7 cut(s) 66, 141, 159, 1161, 1205, 1794, 2532
DpnII GATC 7 cut(s) 64, 139, 157, 1159, 1203, 1792, 2530
EaeI YGGCCR 1 cut(s) 1469
Eam1104I CTCTTC 5 cut(s) 136, 1090, 1124, 1149, 1724
EarI CTCTTC 5 cut(s) 136, 1090, 1124, 1149, 1724
Eco24I GRGCYC 4 cut(s) 1533, 1544, 1672, 1841
Eco31I GGTCTC 1 cut(s) 448
Eco32I GATATC 1 cut(s) 1633
Eco47I GGWCC 2 cut(s) 1495, 1862
Eco57I CTGAAG 2 cut(s) 396, 1311
Eco81I CCTNAGG 2 cut(s) 1185, 2094
EcoNI CCTNNNNNAGG 1 cut(s) 1328
EcoO109I RGGNCCY 2 cut(s) 1540, 1541
EcoRV GATATC 1 cut(s) 1633
EcoT38I GRGCYC 4 cut(s) 1533, 1544, 1672, 1841
FalI AAGNNNNNCTT 4 cut(s) 1253, 1285, 1888, 1920
FaqI GGGAC 4 cut(s) 237, 1502, 2406, 2525
FauI CCCGC 1 cut(s) 253
FbaI TGATCA 3 cut(s) 139, 1203, 1792
FblI GTMKAC 2 cut(s) 1938, 2045
FriOI GRGCYC 4 cut(s) 1533, 1544, 1672, 1841
FspBI CTAG 4 cut(s) 185, 2322, 2471, 2501
GlaI GCGC 3 cut(s) 384, 1221, 1986
GsaI CCCAGC 8 cut(s) 707, 752, 779, 824, 869, 899, 947, 1079
GsuI CTGGAG 4 cut(s) 606, 909, 978, 1935
HaeIII GGCC 4 cut(s) 1471, 1542, 2318, 2434
HapII CCGG 4 cut(s) 1375, 2161, 2279, 2524
HgaI GACGC 2 cut(s) 278, 349
HhaI GCGC 3 cut(s) 385, 1222, 1987
Hin1I GRCGYC 1 cut(s) 341
Hin6I GCGC 3 cut(s) 383, 1220, 1985
HinP1I GCGC 3 cut(s) 383, 1220, 1985
HincII GTYRAC 2 cut(s) 36, 2594
HindII GTYRAC 2 cut(s) 36, 2594
HindIII AAGCTT 2 cut(s) 1787, 2020
HpaII CCGG 4 cut(s) 1375, 2161, 2279, 2524
HphI GGTGA 6 cut(s) 288, 1833, 1950, 2038, 2044, 2185
Hpy166II GTNNAC 4 cut(s) 36, 1939, 2046, 2594
Hpy8I GTNNAC 4 cut(s) 36, 1939, 2046, 2594
Hpy99I CGWCG 1 cut(s) 32
HpyCH4III ACNGT 4 cut(s) 1676, 1730, 1871, 2539
HpyCH4IV ACGT 4 cut(s) 503, 1804, 2107, 2596
HpyF3I CTNAG 6 cut(s) 489, 1185, 1461, 1527, 2094, 2429
HpySE526I ACGT 4 cut(s) 503, 1804, 2107, 2596
Hsp92I GRCGYC 1 cut(s) 341
HspAI GCGC 3 cut(s) 383, 1220, 1985
KpnI GGTACC 2 cut(s) 116, 2093
Ksp22I TGATCA 3 cut(s) 139, 1203, 1792
Kzo9I GATC 7 cut(s) 64, 139, 157, 1159, 1203, 1792, 2530
LmnI GCTCC 1 cut(s) 1563
LweI GCATC 6 cut(s) 244, 680, 899, 1687, 1700, 2248
MaeI CTAG 4 cut(s) 185, 2322, 2471, 2501
MaeII ACGT 4 cut(s) 503, 1804, 2107, 2596
MaeIII GTNAC 8 cut(s) 68, 294, 1316, 1336, 1730, 1855, 1956, 2332
MalI GATC 7 cut(s) 66, 141, 159, 1161, 1205, 1794, 2532
MbiI CCGCTC 2 cut(s) 1277, 2359
MboI GATC 7 cut(s) 64, 139, 157, 1159, 1203, 1792, 2530
MflI RGATCY 2 cut(s) 1159, 2530
MhlI GDGCHC 5 cut(s) 1049, 1533, 1544, 1672, 1841
MluCI AATT 8 cut(s) 123, 163, 511, 1215, 1362, 1583, 1891, 1943
MlyI GAGTC 5 cut(s) 1727, 2051, 2135, 2388, 2412
MmeI TCCRAC 5 cut(s) 1329, 1641, 1795, 1931, 2227
MseI TTAA 4 cut(s) 1059, 1536, 1826, 1895
MspA1I CMGCKG 4 cut(s) 260, 388, 493, 1998
MspCI CTTAAG 1 cut(s) 1825
MspI CCGG 4 cut(s) 1375, 2161, 2279, 2524
MspR9I CCNGG 1 cut(s) 2162
Mva1269I GAATGC 3 cut(s) 1746, 1971, 2069
MvnI CGCG 1 cut(s) 1572
NciI CCSGG 1 cut(s) 2162
NdeII GATC 7 cut(s) 64, 139, 157, 1159, 1203, 1792, 2530
NlaIV GGNNCC 5 cut(s) 114, 1161, 1542, 1543, 2091
NmuCI GTSAC 6 cut(s) 68, 294, 1316, 1336, 1730, 1956
NspI RCATGY 2 cut(s) 27, 903
PagI TCATGA 4 cut(s) 142, 1176, 2190, 2511
PaqCI CACCTGC 2 cut(s) 676, 895
PctI GAATGC 3 cut(s) 1746, 1971, 2069
PfeI GAWTC 7 cut(s) 58, 1180, 1234, 1831, 2035, 2496, 2515
Pfl23II CGTACG 1 cut(s) 207
PflFI GACNNNGTC 1 cut(s) 1493
PfoI TCCNGGA 1 cut(s) 2160
PleI GAGTC 5 cut(s) 1727, 2050, 2134, 2388, 2412
PpsI GAGTC 5 cut(s) 1727, 2050, 2134, 2388, 2412
PsiI TTATAA 2 cut(s) 1368, 1395
Psp1406I AACGTT 1 cut(s) 1804
PspFI CCCAGC 8 cut(s) 703, 748, 775, 820, 865, 895, 943, 1075
PspLI CGTACG 1 cut(s) 207
PspN4I GGNNCC 5 cut(s) 114, 1161, 1542, 1543, 2091
PspOMI GGGCCC 1 cut(s) 1540
PspPI GGNCC 4 cut(s) 1495, 1540, 1541, 1862
PstI CTGCAG 2 cut(s) 850, 1813
PstNI CAGNNNCTG 1 cut(s) 2001
PsuI RGATCY 2 cut(s) 1159, 2530
PsyI GACNNNGTC 1 cut(s) 1493
PvuII CAGCTG 2 cut(s) 388, 1998
RsaI GTAC 4 cut(s) 114, 209, 1522, 2091
RsaNI GTAC 4 cut(s) 113, 208, 1521, 2090
SaqAI TTAA 4 cut(s) 1059, 1536, 1826, 1895
Sau3AI GATC 7 cut(s) 64, 139, 157, 1159, 1203, 1792, 2530
Sau96I GGNCC 4 cut(s) 1495, 1540, 1541, 1862
ScaI AGTACT 1 cut(s) 1522
SchI GAGTC 5 cut(s) 1727, 2051, 2135, 2388, 2412
ScrFI CCNGG 1 cut(s) 2162
SduI GDGCHC 5 cut(s) 1049, 1533, 1544, 1672, 1841
SfaNI GCATC 6 cut(s) 244, 680, 899, 1687, 1700, 2248
SfcI CTRYAG 2 cut(s) 846, 1809
SinI GGWCC 2 cut(s) 1495, 1862
SmlI CTYRAG 3 cut(s) 308, 1825, 2396
SmoI CTYRAG 3 cut(s) 308, 1825, 2396
SpeI ACTAGT 1 cut(s) 2321
Sse9I AATT 8 cut(s) 123, 163, 511, 1215, 1362, 1583, 1891, 1943
SsiI CCGC 7 cut(s) 260, 264, 304, 493, 534, 1275, 2357
SspI AATATT 1 cut(s) 426
SspMI CTAG 4 cut(s) 185, 2322, 2471, 2501
StyD4I CCNGG 1 cut(s) 2160
TaaI ACNGT 4 cut(s) 1676, 1730, 1871, 2539
TaiI ACGT 4 cut(s) 506, 1807, 2110, 2599
TaqI TCGA 2 cut(s) 97, 1194
TasI AATT 8 cut(s) 123, 163, 511, 1215, 1362, 1583, 1891, 1943
TatI WGTACW 1 cut(s) 1520
TauI GCSGC 2 cut(s) 496, 1277
TfiI GAWTC 7 cut(s) 58, 1180, 1234, 1831, 2035, 2496, 2515
Tru1I TTAA 4 cut(s) 1059, 1536, 1826, 1895
Tru9I TTAA 4 cut(s) 1059, 1536, 1826, 1895
TscAI CASTG 1 cut(s) 1515
TseFI GTSAC 6 cut(s) 68, 294, 1316, 1336, 1730, 1956
Tsp45I GTSAC 6 cut(s) 68, 294, 1316, 1336, 1730, 1956
TspRI CASTG 1 cut(s) 1515
Tth111I GACNNNGTC 1 cut(s) 1493
Vha464I CTTAAG 1 cut(s) 1825
VpaK11BI GGWCC 2 cut(s) 1495, 1862
XagI CCTNNNNNAGG 1 cut(s) 1328
XapI RAATTY 3 cut(s) 163, 1583, 1891
XceI RCATGY 2 cut(s) 27, 903
XcmI CCANNNNNNNNNTGG 2 cut(s) 1556, 2489
XmiI GTMKAC 2 cut(s) 1938, 2045
XspI CTAG 4 cut(s) 185, 2322, 2471, 2501
ZrmI AGTACT 1 cut(s) 1522
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.