Rorug01G0019600

RNA helicase SDE3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0000595
Physical Location & Seq
Reverse (-)
3237904 .. 3245292
7389 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0019600.1

Sequence Viewer

Length: 4422 bp
ATGGTGGTCGAGAAGAAGCGCCCGCTGCAGAGCAGCAACAACGCCAACACGGCGGCGCCGCCTTCGACGGACGGCGGCCACGGCAAGACGATCGAGGAAATGTACCAGAAGAAGAGCCAGCTGGAGCACATCTTGCTCCGACCCGATACCTACATCGGATCCATAGAGAAGCACACCCAAACCCTCTGGGTCTTCGACAACGACGAGATGGTCCAGCGCTCCGTCTCCTACGTCCCCGGCCTCTACAAGATCTTCGACGAGATTCTCGTCAACGCCGCCGACAACAAACAGCGAGATCCGTCCATGGACGCTCTCAAGGTCACCATCGACGTCGAGCAGAACTGCATCAGTGTGTACAACAACGGCGACGGAGTTCCGGTGGAGATTCACCAGGAGGAGGGGGTTTATGTGCCGGAGTTGATCTTCGGGCACCTACTGACTAGTAGTAACTACGACGACACGGTGAAGAAGACCACCGGTGGACGCAACGGCTACGGCGCCAAGCTCACTAATATATTCTCCACCGAATTCGTCATCGAAACCGCCGACGGCAAGCGGCTGAAGAAGTACAAGCAGGTGTTCACAAACAACATGGGGAAGAAATCTGAACCGGTCATCACTAAATGCAAAGAGAGTGAGAACTGGACCCGGGTGACGTATAAGCCTGATTTGACAAAATTTAACATGACACATCTTGAAGATGATGTGGTAGCATTGATGAAAAAGCGAGTGATTGACATGGCTGGATGCCTTGGAAAGACTGTGAAGGTCGATCTGAATGGAAAGAGGGTCCCTGTAAAGTCGTTTCTTGATTATGTTGATCTCTACTTGCAATCTGCCGGAAAATCTAAAGATACTAAACTTCCAAGGATGACTGAGAAAGTCAATGATAGGTGGGAGATTTGTGTGAGTCTCAGTGACGGGCAGTTTCAACAGGTCAGCTTTGTGAATAGCATTGCTACAATCAAGGGGGGAACTCATGTTGATTATGTCACAAACCAGATTACTGCTCATGTGATGAATGTTGTAAATAAGAAGAACAAAAATGCCAACCTCAAGGCTCACAACGTGAAGAATTATCTTTGGGTCTTTGTCAATGCTCTTATTGACAACCCTGCCTTTGATTCCCAAACAAAGGAAACTTTGACGATTCGCCCAAGCAGTTTTGGGTCTAAATGTGAACTACCGCAAGAGTTCTTAAAGAAAGTTGCAAAGTCTGGGGTTGTGGAAAGTCTTCTCTCTTGGGCAAGTTTTAAGCAGAACAAAGATCTGAAGAAAACTGATGGAACAAAAACGGAGAGGCTTCATAATATCAACAAGCTGGAGGATGCTAACTTAGCAGGAGGAAGGAACTCTGAAAAGTGTACCTTGATTTTAACAGAGGGAGATTCAGCTAAGGCTCTTGCAATGGCGGGACTGGCTGTTGTTGGTCGGGACCATTATGGTGTTTTTCCATTGAGAGGTAAATTGCTTAATGTAAGAGAAGCCAGCGCGACTCAAGTCAGGGATAATGAAGAAATCAAGAATATTAAGAAGATTCTTGGTCTGCAGCAAGATAAACAATATACCAACATCAAATCTTTAAGATATGGGCATCTGATGATCATGACCGATCAGGATCACGATGGCTCCCATATAAAAGGGCTGCTGATCAATTTCATTCATTCCTTCTGGCCCTCTCTGCTAAAAGTTCCATCATTTTTGGTTGAGTTTATAACGCCTATTGTTAAGGCAACTCAGAAGAATGGGAAAGAACTTGCTTTTTATTCAATGCCCGAGTATGAAGCTTGGAGGGAAAGCTTGAGGGGTAATGCAAGTGGGTGGACCATAAAATACTATAAGGGGTTGGGAACAAGTACTTCAAAGGAAGGCAGGGACTACTTTCAGAATCTAGATATGCACAAGAAAGACTTCGTTTGGATAGATGAACAGGATGGGGAAGCAATTGAACTCGCTTTTAGCAAGAAGAAAATAGAAGAGAGGAAGAATTGGCTTCGGCAGTTTGAGCCTGGTACTCATCTTGATCAGAAGGCGAAGCTTGTAAAGTACAGTGACTTTGTTCACAAGGAGCTTATACAGTTTTCCATGGCGGATCTGCAAAGGTCAATACCATCAATGGTTGATGGGTTGAAGCCAGGTCAGAGAAAGATACTATTCTGCTCTTTTAAGAGGAACTTTATCAAGGAAGCAAAAGTTGCCCAGTTTTCTGGTTATGTGTCTGAGCATTCTGCCTACCACCATGGTGAACAAAGTCTTGCCAGTACTATCATTGGAATGGCACAAGATTATGTGGGCAGTAACAACATTAATCTCCTTCAACCAAATGGTCAATTTGGGACCCGTAACTTGGGAGGCAAAGACCATGCAAGTGCTCGTTATATATATACTAAGCTTTCTCCTATTACAAGGTTCCTTTTTCCAAAGGATGATGATAGACTTCTTGACTACTTGAATGAAGATGGGCAATCTATTGAGCCAACCTGGTATGTGCCAGTTATCCCAACAGTTCTTGTGAATGGTAGTGAAGGAATTGGGACAGGGTGGAGCTCTTACATCCCTAATTATAATCCAAGAGACATTGTTGCAAACGTTAGGCGGTTGCTCAATGGTGATGAAATGGTGCCAATGGATCCTTGGTATAGAGGCTTTACTGGATTTATTGAGAAAACTGTCAAGGAAACTGGTCCAAGCTACACAGTATGTGGGATTATTGAGGAAGTCAATGAGACAACACTCAGGATTTCGGAGTTACCAATCCGTAGGTGGACTCAAGATTATAAGGAATTTCTGGAGTCTATCTCTCAAGGGAATGACAAGGCCAAGGATCCTTTGATCGAGGGTTTCACACAGCATAGTGATCATAGTACTGTAGAAATTCTAGTCCACTTGCCTGAGGAGAACCTGATGGCAGCCAGAAAAGAGGGTTTGCTGAAGAAGTTTAAATTGACCACTAGCATGGGCACAACAAACATGCATCTGTTTGATCCAAAGGGTGTGATTAAGAAATATGACACCCCAGAACAAATTCTTGAGGAGTTCTTTCACCTAAGACTTGAATTCTATGAGAAAAGAAAGAAAGTATTGTTGGATAACCTTGAGTTGGAATTGTTGAAATTGGAAAACAAGGTTAGATTCATCCTTGGTGTGGTGAATGGAGAAATTATTGTGAGTAATAGGAAGAGAGCTGATCTATTCCTTGAGCTCCAACAGAAAGGTTTCACTCCTTTTCCGAAGAAAACTAAGGCTGTGGAGCCAGAGGTTGCTGGGGCAACTGAAGATACAGATGAAACAGAAGAGAACTCAGAAGCTGTCATTGGTAATGGAGTACGAATTAGCGATTATGAGTACCTAATATCCATGGCAATTGGAACCTTGACCATTGAGAGGGTTCAAGCTTTATTGGCTGATAGGGATAAGGTCAATGAGCAGGTTACTGATTTGAGAGGTTCAACTCCAAAGTCACTGTGGTTGAAGGATCTTGATGCTCTTGAATTGGAACTTGATGAGCTGGAGAAAAGTGAGGCTCTGGCAGATGAGGCAAAAAGGAAATCAAGAAACAACCAAATCAAGAATGCGCCTGTTGGGAAGGTTTCTAAACAAGCACCTAAGCCACGAAAGAATACTAAAAAGGCTAACAATGCAGAAGCTATTGCAGACAGTAGGGAAACATCTTCCAGTTCTGCAATGGAAATGGAGAAAGCTCCTGAGGTGGTGAAACGGAGAGGTGCAGCGGCAGGTTCAAAGAAAGCTCCTGTTAAGAAGCAAGCAAAATCCACAGTAGTTTTGTCTGATGATGAAGATGAAGAAATGCTTGATTTGAAAGATCGACTGGCTGCCTATAATCTTGATTCATCTCCAGACAAGGAAACTGAAGTGCCACCAAAAGCAGCCCAGAAGAAGGCAGCTACCAAAAAGGCCACAATAACAGACGATGAGGACGATGACGATGACGATTTTGAGGTGGAAATAGCAGCTGCCCCCGCAGCAGTTAAGAAGGGAGGTAGAAAGCCTGCTGGAGCTACGAAGGCAGCAGCAGCGGCTAAGCCCCCTGTGGCAGCCAAGAAGAGAGGTCCAGCGGCGAAGAAGGAGTCTCAAACATCATCATCTTCGGGCCAGAAGCTCTTAACTGATATGTTGAAGCCTGCTGCTGAGAGTTCTGGGGTTTCTCCCGACCCAAAAGTGAGAAAGATGAGGGAATCTCCATTCAACAAGAAGAGTGGTTCTGTGTTGAGCCGGGTTAACTTGGAAGATGAGATGAATGAAAGTGAAGAGAAAACCGAAGCTCCAGTAGCAGCTGGAAGAGCTAGACCGCAGAGGGCGGCTGCTCGTAACAGGAAAATGGTTCTGTCAAGTGACTCTGAGGATGCAAATGATAGTGATGAGTCTGAGGTGGAGGAAATTGATTCCGACGATTCTGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1473

Amino Acids

164.99

Weight (kDa)

6.88

Isoelectric Point (pI)

42.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HATPase_c PF02518 80 - 174 3.5e-07 Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
DNA_gyraseB PF00204 270 - 426 5.6e-25 DNA gyrase B
Toprim PF01751 456 - 555 1.1e-08 Toprim domain
TOPRIM_C PF16898 571 - 698 1.3e-51 C-terminal associated domain of TOPRIM
DNA_topoisoIV PF00521 701 - 1160 2.9e-118 DNA gyrase/topoisomerase IV, subunit A
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000595)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g37100 FvH4_4g37100 FvH4_4g37100
malus_domestica MD13G1238300.v1.1 MD16G1243100.v1.1 MD16G1243200.v1.1
prunus_persica Prupe.1G070100_v2.0.a1 Prupe.1G070200_v2.0.a1 Prupe.1G070300_v2.0.a1 Prupe.1G070300_v2.0.a1
pyrus_communis pycom13g21070 pycom16g20390 pycom16g20400
rosa_chinensis RchiOBHm_Chr1g0318071 RchiOBHm_Chr1g0318111 RchiOBHm_Chr4g0446861 RchiOBHm_Chr4g0446881 RchiOBHm_Chr4g0446891
rosa_laevigata RLG00000005623 RLG00000005624 RLG00000009337 RLG00000030560 RLG00000030566
rosa_multiflora Rmu_co8177450.1_g000001 Rmu_sc0001753.1_g000012 Rmu_sc0001753.1_g000013 Rmu_sc0001753.1_g000014 Rmu_sc0001753.1_g000015 Rmu_sc0011424.1_g000009 Rmu_sc0011424.1_g000020 Rmu_sc0034555.1_g000001 Rmu_ssc0000008.1_g000002
rosa_roxburghii Rroxscaffold_4G00329530 Rroxscaffold_4G00329590 Rroxscaffold_4G00329800 Rroxscaffold_4G00329810 Rroxscaffold_4G00329860 Rroxscaffold_4G00329910 Rroxscaffold_4G00330030 Rroxscaffold_5G00343610 Rroxscaffold_5G00348280 Rroxscaffold_5G00387220 Rroxscaffold_5G00387230
rosa_rugosa Rorug01G0019100 Rorug01G0019100 Rorug01G0019500 Rorug01G0019600 Rorug04G0010100 Rorug04G0375200 Rorug04G0375300 Rorug04G0375400 Rorug04G0375500 Rorug04G0375600 Rorug04G0375700
rosa_samantha Rh1AG030700 Rh1AG030900 Rh1BG026900 Rh1CG029500 Rh1CG029700 Rh1DG040500 Rh4AG088100 Rh4AG436700 Rh4AG436800 Rh4AG436900 Rh4BG084700 Rh4BG445800 Rh4BG445900 Rh4BG446100 Rh4CG096300 Rh4CG463100 Rh4CG463200 Rh4CG463400 Rh4DG444500 Rh4DG444600 Rh4DG444700
rosa_wichuraiana Rw1G002420 Rw1G002440 Rw1G002480 Rw4G037360 Rw4G037370 Rw4G037380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 1715, 2565, 2778
AarI CACCTGC 1 cut(s) 565
AasI GACNNNNNNGTC 1 cut(s) 209
AatII GACGTC 1 cut(s) 333
Acc36I ACCTGC 3 cut(s) 565, 3418, 3725
AccB1I GGYRCC 4 cut(s) 55, 429, 497, 2620
AccII CGCG 1 cut(s) 1493
AclI AACGTT 1 cut(s) 2589
AcoI YGGCCR 1 cut(s) 76
AcsI RAATTY 6 cut(s) 527, 677, 2783, 2874, 3024, 3056
AcuI CTGAAG 5 cut(s) 581, 1292, 2951, 3294, 3891
AcyI GRCGYC 3 cut(s) 56, 330, 498
AdeI CACNNNGTG 1 cut(s) 1069
AfeI AGCGCT 1 cut(s) 218
AfiI CCNNNNNNNGG 8 cut(s) 397, 1135, 1460, 2347, 3100, 3145, 3384, 3898
AgeI ACCGGT 2 cut(s) 476, 610
AhlI ACTAGT 1 cut(s) 440
AjnI CCWGG 4 cut(s) 390, 2008, 2134, 2480
AjuI GAANNNNNNNTTGG 4 cut(s) 3068, 3100, 3297, 3329
AleI CACNNNNGTG 1 cut(s) 2241
Alw21I GWGCWC 4 cut(s) 129, 2374, 2549, 3204
Alw26I GTCTC 5 cut(s) 229, 917, 2568, 2720, 4095
AlwNI CAGNNNCTG 1 cut(s) 3308
Ama87I CYCGRG 2 cut(s) 648, 1775
Aor51HI AGCGCT 1 cut(s) 218
AoxI GGCC 6 cut(s) 76, 238, 1673, 2817, 3915, 4111
ApoI RAATTY 6 cut(s) 527, 677, 2783, 2874, 3024, 3056
ArsI GACNNNNNNTTYG 2 cut(s) 4073, 4105
AseI ATTAAT 1 cut(s) 2307
AsiGI ACCGGT 2 cut(s) 476, 610
Asp700I GAANNNNTTC 4 cut(s) 1233, 1910, 3024, 3215
AspLEI GCGC 6 cut(s) 21, 58, 219, 500, 1493, 3577
AsuC2I CCSGG 4 cut(s) 237, 649, 650, 4235
AsuHPI GGTGA 9 cut(s) 313, 380, 475, 664, 2255, 2621, 3035, 3160, 3724
AvaI CYCGRG 2 cut(s) 648, 1775
AvaII GGWCC 8 cut(s) 211, 645, 790, 1435, 1824, 2337, 2684, 4070
AxyI CCTNAGG 2 cut(s) 2892, 3705
BaeGI GKGCMC 2 cut(s) 432, 2963
BamHI GGATCC 3 cut(s) 158, 2629, 2824
BanI GGYRCC 4 cut(s) 55, 429, 497, 2620
BanII GRGCYC 2 cut(s) 2549, 3204
BbsI GAAGAC 3 cut(s) 184, 476, 1226
Bbv12I GWGCWC 4 cut(s) 129, 2374, 2549, 3204
BceAI ACGGC 7 cut(s) 66, 88, 97, 379, 505, 511, 565
BcgI CGANNNNNNTGC 2 cut(s) 73, 107
BciT130I CCWGG 4 cut(s) 392, 2010, 2136, 2482
BclI TGATCA 4 cut(s) 1602, 1650, 2023, 2857
BcnI CCSGG 4 cut(s) 237, 649, 650, 4235
BcoDI GTCTC 5 cut(s) 229, 917, 2568, 2720, 4095
BcuI ACTAGT 1 cut(s) 440
BfaI CTAG 5 cut(s) 441, 1892, 2879, 2952, 4305
BfmI CTRYAG 3 cut(s) 26, 1547, 2868
BfoI RGCGCY 4 cut(s) 22, 59, 220, 501
BfuAI ACCTGC 3 cut(s) 565, 3418, 3725
BglI GCCNNNNNGGC 1 cut(s) 50
BglII AGATCT 2 cut(s) 249, 1267
BlpI GCTNAGC 1 cut(s) 4041
BmcAI AGTACT 3 cut(s) 1858, 2263, 2866
Bme1390I CCNGG 8 cut(s) 237, 392, 649, 650, 2010, 2136, 2482, 4235
Bme18I GGWCC 8 cut(s) 211, 645, 790, 1435, 1824, 2337, 2684, 4070
BmeT110I CYCGRG 2 cut(s) 648, 1775
BmrFI CCNGG 8 cut(s) 237, 392, 649, 650, 2010, 2136, 2482, 4235
BmrI ACTGGG 1 cut(s) 2194
BmsI GCATC 7 cut(s) 354, 737, 1318, 1603, 2983, 3472, 4354
BmuI ACTGGG 1 cut(s) 2194
BoxI GACNNNNGTC 1 cut(s) 1499
BpiI GAAGAC 3 cut(s) 184, 476, 1226
BplI GAGNNNNNCTC 4 cut(s) 2783, 2815, 4183, 4215
BpmI CTGGAG 7 cut(s) 143, 1343, 2810, 3530, 3840, 4035, 4269
Bpu10I CCTNAGC 2 cut(s) 1395, 3606
Bpu1102I GCTNAGC 1 cut(s) 4041
BpuEI CTTGAG 9 cut(s) 299, 1040, 1482, 1822, 2754, 2787, 3050, 3116, 3218
BpuMI CCSGG 4 cut(s) 237, 649, 650, 4235
BsaBI GATNNNNATC 1 cut(s) 1617
BsaHI GRCGYC 3 cut(s) 56, 330, 498
BsaWI WCCGGW 3 cut(s) 376, 476, 610
BsaXI ACNNNNNCTCC 6 cut(s) 363, 393, 1613, 1643, 4267, 4297
Bsc4I CCNNNNNNNGG 8 cut(s) 397, 1135, 1460, 2347, 3100, 3145, 3384, 3898
Bse118I RCCGGY 2 cut(s) 476, 610
Bse21I CCTNAGG 2 cut(s) 2892, 3705
Bse3DI GCAATG 3 cut(s) 954, 1413, 3690
Bse8I GATNNNNATC 1 cut(s) 1617
BseBI CCWGG 4 cut(s) 392, 2010, 2136, 2482
BseGI GGATG 8 cut(s) 752, 876, 1333, 1939, 2431, 2553, 3135, 4369
BseJI GATNNNNATC 1 cut(s) 1617
BseLI CCNNNNNNNGG 8 cut(s) 397, 1135, 1460, 2347, 3100, 3145, 3384, 3898
BseMI GCAATG 3 cut(s) 954, 1413, 3690
BseRI GAGGAG 3 cut(s) 410, 2909, 3047
BseSI GKGCMC 2 cut(s) 432, 2963
BseYI CCCAGC 1 cut(s) 3263
BsgI GTGCAG 1 cut(s) 3747
Bsh1236I CGCG 1 cut(s) 1493
Bsh1285I CGRYCG 1 cut(s) 93
BshFI GGCC 6 cut(s) 78, 240, 1675, 2819, 3917, 4113
BshNI GGYRCC 4 cut(s) 55, 429, 497, 2620
BshTI ACCGGT 2 cut(s) 476, 610
BsiEI CGRYCG 1 cut(s) 93
BsiHKAI GWGCWC 4 cut(s) 129, 2374, 2549, 3204
BsiHKCI CYCGRG 2 cut(s) 648, 1775
BsiSI CCGG 8 cut(s) 237, 377, 413, 477, 611, 649, 840, 4234
BslFI GGGAC 7 cut(s) 218, 776, 1428, 1448, 1889, 2350, 2548
BslI CCNNNNNNNGG 8 cut(s) 397, 1135, 1460, 2347, 3100, 3145, 3384, 3898
BsmAI GTCTC 5 cut(s) 229, 917, 2568, 2720, 4095
BsmBI CGTCTC 1 cut(s) 229
BsmFI GGGAC 7 cut(s) 218, 776, 1428, 1448, 1889, 2350, 2548
BsmI GAATGC 2 cut(s) 2224, 3577
BsnI GGCC 6 cut(s) 78, 240, 1675, 2819, 3917, 4113
BsoBI CYCGRG 2 cut(s) 648, 1775
Bsp1286I GDGCHC 6 cut(s) 129, 432, 2374, 2549, 2963, 3204
Bsp1407I TGTACA 1 cut(s) 354
Bsp1720I GCTNAGC 1 cut(s) 4041
Bsp19I CCATGG 4 cut(s) 303, 2085, 2239, 3357
BspANI GGCC 6 cut(s) 78, 240, 1675, 2819, 3917, 4113
BspFNI CGCG 1 cut(s) 1493
BspHI TCATGA 1 cut(s) 1605
BspMAI CTGCAG 2 cut(s) 30, 1551
BspMI ACCTGC 3 cut(s) 565, 3418, 3725
BspQI GCTCTTC 2 cut(s) 107, 4294
BspT107I GGYRCC 4 cut(s) 55, 429, 497, 2620
BsrDI GCAATG 3 cut(s) 954, 1413, 3690
BsrFI RCCGGY 2 cut(s) 476, 610
BsrGI TGTACA 1 cut(s) 354
BssAI RCCGGY 2 cut(s) 476, 610
BssNI GRCGYC 3 cut(s) 56, 330, 498
BssT1I CCWWGG 9 cut(s) 303, 751, 866, 2085, 2239, 2633, 2820, 3139, 3357
Bst2UI CCWGG 4 cut(s) 392, 2010, 2136, 2482
Bst6I CTCTTC 8 cut(s) 107, 1971, 3173, 3288, 4058, 4208, 4263, 4294
BstACI GRCGYC 3 cut(s) 56, 330, 498
BstAPI GCANNNNNTGC 2 cut(s) 133, 2195
BstAUI TGTACA 1 cut(s) 354
BstC8I GCNNGC 7 cut(s) 23, 119, 554, 1489, 3765, 4011, 4143
BstDSI CCRYGG 5 cut(s) 79, 303, 2085, 2239, 3357
BstEII GGTNACC 1 cut(s) 319
BstF5I GGATG 8 cut(s) 752, 876, 1333, 1939, 2431, 2553, 3135, 4369
BstFNI CGCG 1 cut(s) 1493
BstH2I RGCGCY 4 cut(s) 22, 59, 220, 501
BstHHI GCGC 6 cut(s) 21, 58, 219, 500, 1493, 3577
BstMAI GTCTC 5 cut(s) 229, 917, 2568, 2720, 4095
BstMCI CGRYCG 1 cut(s) 93
BstNI CCWGG 4 cut(s) 392, 2010, 2136, 2482
BstNSI RCATGY 1 cut(s) 2974
BstPAI GACNNNNGTC 1 cut(s) 1499
BstPI GGTNACC 1 cut(s) 319
BstSCI CCNGG 8 cut(s) 235, 390, 647, 648, 2008, 2134, 2480, 4233
BstSFI CTRYAG 3 cut(s) 26, 1547, 2868
BstSLI GKGCMC 2 cut(s) 432, 2963
BstUI CGCG 1 cut(s) 1493
BstV2I GAAGAC 3 cut(s) 184, 476, 1226
BstX2I RGATCY 8 cut(s) 158, 249, 295, 1267, 2092, 2629, 2824, 3475
BstXI CCANNNNNNTGG 2 cut(s) 2207, 2956
BstYI RGATCY 8 cut(s) 158, 249, 295, 1267, 2092, 2629, 2824, 3475
Bsu36I CCTNAGG 2 cut(s) 2892, 3705
BsuRI GGCC 6 cut(s) 78, 240, 1675, 2819, 3917, 4113
BtgI CCRYGG 5 cut(s) 79, 303, 2085, 2239, 3357
BtsCI GGATG 8 cut(s) 752, 876, 1333, 1939, 2431, 2553, 3135, 4369
BtsIMutI CAGTG 4 cut(s) 355, 922, 2056, 3461
BveI ACCTGC 3 cut(s) 565, 3418, 3725
Cac8I GCNNGC 7 cut(s) 23, 119, 554, 1489, 3765, 4011, 4143
CaiI CAGNNNCTG 1 cut(s) 3308
CciI TCATGA 1 cut(s) 1605
CfoI GCGC 6 cut(s) 21, 58, 219, 500, 1493, 3577
Cfr10I RCCGGY 2 cut(s) 476, 610
Cfr9I CCCGGG 1 cut(s) 648
CseI GACGC 2 cut(s) 317, 492
CsiI ACCWGGT 1 cut(s) 2480
CspAI ACCGGT 2 cut(s) 476, 610
CspCI CAANNNNNGTGG 2 cut(s) 4198, 4233
DinI GGCGCC 2 cut(s) 57, 499
DraI TTTAAA 1 cut(s) 2941
DraIII CACNNNGTG 1 cut(s) 1069
DrdI GACNNNNNNGTC 1 cut(s) 209
DseDI GACNNNNNNGTC 1 cut(s) 209
EaeI YGGCCR 1 cut(s) 76
Eam1104I CTCTTC 8 cut(s) 107, 1971, 3173, 3288, 4058, 4208, 4263, 4294
EarI CTCTTC 8 cut(s) 107, 1971, 3173, 3288, 4058, 4208, 4263, 4294
EciI GGCGGA 1 cut(s) 2105
Ecl136II GAGCTC 2 cut(s) 2547, 3202
Eco130I CCWWGG 9 cut(s) 303, 751, 866, 2085, 2239, 2633, 2820, 3139, 3357
Eco24I GRGCYC 2 cut(s) 2549, 3204
Eco47I GGWCC 8 cut(s) 211, 645, 790, 1435, 1824, 2337, 2684, 4070
Eco47III AGCGCT 1 cut(s) 218
Eco53kI GAGCTC 2 cut(s) 2547, 3202
Eco57I CTGAAG 5 cut(s) 581, 1292, 2951, 3294, 3891
Eco81I CCTNAGG 2 cut(s) 2892, 3705
Eco88I CYCGRG 2 cut(s) 648, 1775
Eco91I GGTNACC 1 cut(s) 319
EcoICRI GAGCTC 2 cut(s) 2547, 3202
EcoO109I RGGNCCY 2 cut(s) 790, 2337
EcoO65I GGTNACC 1 cut(s) 319
EcoRI GAATTC 2 cut(s) 527, 3056
EcoRII CCWGG 4 cut(s) 390, 2008, 2134, 2480
EcoT14I CCWWGG 9 cut(s) 303, 751, 866, 2085, 2239, 2633, 2820, 3139, 3357
EcoT22I ATGCAT 1 cut(s) 2976
EcoT38I GRGCYC 2 cut(s) 2549, 3204
EgeI GGCGCC 2 cut(s) 57, 499
EheI GGCGCC 2 cut(s) 57, 499
ErhI CCWWGG 9 cut(s) 303, 751, 866, 2085, 2239, 2633, 2820, 3139, 3357
Esp3I CGTCTC 1 cut(s) 229
FalI AAGNNNNNCTT 8 cut(s) 1352, 1384, 1895, 1927, 2159, 2191, 3795, 3827
FaqI GGGAC 7 cut(s) 218, 776, 1428, 1448, 1889, 2350, 2548
FauI CCCGC 3 cut(s) 30, 1405, 3988
FbaI TGATCA 4 cut(s) 1602, 1650, 2023, 2857
FokI GGATG 8 cut(s) 759, 883, 1340, 1946, 2438, 2540, 3122, 4376
FriOI GRGCYC 2 cut(s) 2549, 3204
FspBI CTAG 5 cut(s) 441, 1892, 2879, 2952, 4305
GlaI GCGC 6 cut(s) 20, 57, 218, 499, 1492, 3576
GsaI CCCAGC 1 cut(s) 3267
GsuI CTGGAG 7 cut(s) 143, 1343, 2810, 3530, 3840, 4035, 4269
HaeII RGCGCY 4 cut(s) 22, 59, 220, 501
HaeIII GGCC 6 cut(s) 78, 240, 1675, 2819, 3917, 4113
HapII CCGG 8 cut(s) 237, 377, 413, 477, 611, 649, 840, 4234
HgaI GACGC 2 cut(s) 317, 492
HhaI GCGC 6 cut(s) 21, 58, 219, 500, 1493, 3577
Hin1I GRCGYC 3 cut(s) 56, 330, 498
Hin6I GCGC 6 cut(s) 19, 56, 217, 498, 1491, 3575
HinP1I GCGC 6 cut(s) 19, 56, 217, 498, 1491, 3575
HincII GTYRAC 2 cut(s) 271, 4240
HindII GTYRAC 2 cut(s) 271, 4240
HindIII AAGCTT 5 cut(s) 1785, 1798, 2036, 2390, 3393
HpaI GTTAAC 1 cut(s) 4240
HpaII CCGG 8 cut(s) 237, 377, 413, 477, 611, 649, 840, 4234
HphI GGTGA 9 cut(s) 313, 380, 475, 664, 2255, 2621, 3035, 3160, 3724
Hpy99I CGWCG 9 cut(s) 70, 206, 260, 332, 335, 371, 458, 551, 4412
HpyCH4IV ACGT 5 cut(s) 231, 330, 656, 1068, 2589
HpySE526I ACGT 5 cut(s) 231, 330, 656, 1068, 2589
Hsp92I GRCGYC 3 cut(s) 56, 330, 498
HspAI GCGC 6 cut(s) 19, 56, 217, 498, 1491, 3575
KasI GGCGCC 2 cut(s) 55, 497
KflI GGGWCCC 2 cut(s) 790, 2337
Ksp22I TGATCA 4 cut(s) 1602, 1650, 2023, 2857
KspAI GTTAAC 1 cut(s) 4240
LguI GCTCTTC 2 cut(s) 107, 4294
LweI GCATC 7 cut(s) 354, 737, 1318, 1603, 2983, 3472, 4354
MabI ACCWGGT 1 cut(s) 2480
MaeI CTAG 5 cut(s) 441, 1892, 2879, 2952, 4305
MaeII ACGT 5 cut(s) 231, 330, 656, 1068, 2589
MfeI CAATTG 2 cut(s) 1944, 3363
MflI RGATCY 8 cut(s) 158, 249, 295, 1267, 2092, 2629, 2824, 3475
MhlI GDGCHC 6 cut(s) 129, 432, 2374, 2549, 2963, 3204
Mly113I GGCGCC 2 cut(s) 56, 498
MlyI GAGTC 7 cut(s) 919, 1489, 2761, 2801, 4097, 4349, 4391
MmeI TCCRAC 4 cut(s) 163, 3066, 3081, 3229
Mph1103I ATGCAT 1 cut(s) 2976
MroXI GAANNNNTTC 4 cut(s) 1233, 1910, 3024, 3215
MslI CAYNNNNRTG 6 cut(s) 350, 1443, 2241, 2273, 2367, 2954
MspA1I CMGCKG 7 cut(s) 25, 121, 3731, 3974, 4037, 4076, 4295
MspI CCGG 8 cut(s) 237, 377, 413, 477, 611, 649, 840, 4234
MspR9I CCNGG 8 cut(s) 237, 392, 649, 650, 2010, 2136, 2482, 4235
MunI CAATTG 2 cut(s) 1944, 3363
Mva1269I GAATGC 2 cut(s) 2224, 3577
MvaI CCWGG 4 cut(s) 392, 2010, 2136, 2482
MvnI CGCG 1 cut(s) 1493
NarI GGCGCC 2 cut(s) 56, 498
NciI CCSGG 4 cut(s) 237, 649, 650, 4235
NcoI CCATGG 4 cut(s) 303, 2085, 2239, 3357
NmuCI GTSAC 7 cut(s) 319, 652, 917, 991, 2051, 3459, 4352
NsiI ATGCAT 1 cut(s) 2976
NspI RCATGY 1 cut(s) 2974
OliI CACNNNNGTG 1 cut(s) 2241
PagI TCATGA 1 cut(s) 1605
PaqCI CACCTGC 1 cut(s) 565
PciSI GCTCTTC 2 cut(s) 107, 4294
PcsI WCGNNNNNNNCGW 4 cut(s) 201, 264, 543, 3936
PctI GAATGC 2 cut(s) 2224, 3577
PdmI GAANNNNTTC 4 cut(s) 1233, 1910, 3024, 3215
PinAI ACCGGT 2 cut(s) 476, 610
Ple19I CGATCG 1 cut(s) 93
PleI GAGTC 7 cut(s) 918, 1489, 2761, 2800, 4096, 4349, 4390
PluTI GGCGCC 2 cut(s) 59, 501
PpsI GAGTC 7 cut(s) 918, 1489, 2761, 2800, 4096, 4349, 4390
PpuMI RGGWCCY 2 cut(s) 790, 2337
PshAI GACNNNNGTC 1 cut(s) 1499
PshBI ATTAAT 1 cut(s) 2307
PsiI TTATAA 3 cut(s) 1715, 2565, 2778
Psp124BI GAGCTC 2 cut(s) 2549, 3204
Psp1406I AACGTT 1 cut(s) 2589
Psp5II RGGWCCY 2 cut(s) 790, 2337
Psp6I CCWGG 4 cut(s) 390, 2008, 2134, 2480
PspEI GGTNACC 1 cut(s) 319
PspFI CCCAGC 1 cut(s) 3263
PspGI CCWGG 4 cut(s) 390, 2008, 2134, 2480
PspPPI RGGWCCY 2 cut(s) 790, 2337
PstI CTGCAG 2 cut(s) 30, 1551
PstNI CAGNNNCTG 1 cut(s) 3308
PsuI RGATCY 8 cut(s) 158, 249, 295, 1267, 2092, 2629, 2824, 3475
PvuI CGATCG 1 cut(s) 93
PvuII CAGCTG 3 cut(s) 121, 3974, 4295
RseI CAYNNNNRTG 6 cut(s) 350, 1443, 2241, 2273, 2367, 2954
SacI GAGCTC 2 cut(s) 2549, 3204
SapI GCTCTTC 2 cut(s) 107, 4294
ScaI AGTACT 3 cut(s) 1858, 2263, 2866
SchI GAGTC 7 cut(s) 919, 1489, 2761, 2801, 4097, 4349, 4391
ScrFI CCNGG 8 cut(s) 237, 392, 649, 650, 2010, 2136, 2482, 4235
SduI GDGCHC 6 cut(s) 129, 432, 2374, 2549, 2963, 3204
SexAI ACCWGGT 1 cut(s) 2480
SfaNI GCATC 7 cut(s) 354, 737, 1318, 1603, 2983, 3472, 4354
SfcI CTRYAG 3 cut(s) 26, 1547, 2868
SfoI GGCGCC 2 cut(s) 57, 499
SgrAI CRCCGGYG 1 cut(s) 476
SinI GGWCC 8 cut(s) 211, 645, 790, 1435, 1824, 2337, 2684, 4070
SmaI CCCGGG 1 cut(s) 650
SmiMI CAYNNNNRTG 6 cut(s) 350, 1443, 2241, 2273, 2367, 2954
SmlI CTYRAG 9 cut(s) 314, 1055, 1497, 1801, 2769, 2802, 3029, 3095, 3197
SmoI CTYRAG 9 cut(s) 314, 1055, 1497, 1801, 2769, 2802, 3029, 3095, 3197
SpeI ACTAGT 1 cut(s) 440
SspDI GGCGCC 2 cut(s) 55, 497
SspI AATATT 1 cut(s) 1528
SspMI CTAG 5 cut(s) 441, 1892, 2879, 2952, 4305
SstI GAGCTC 2 cut(s) 2549, 3204
StyD4I CCNGG 8 cut(s) 235, 390, 647, 648, 2008, 2134, 2480, 4233
StyI CCWWGG 9 cut(s) 303, 751, 866, 2085, 2239, 2633, 2820, 3139, 3357
TaiI ACGT 5 cut(s) 234, 333, 659, 1071, 2592
TaqII GACCGA 1 cut(s) 1625
TatI WGTACW 6 cut(s) 354, 567, 1856, 2046, 2261, 2864
TauI GCSGC 9 cut(s) 56, 61, 78, 278, 559, 3734, 4040, 4079, 4322
TscAI CASTG 4 cut(s) 355, 922, 2056, 3468
TseFI GTSAC 7 cut(s) 319, 652, 917, 991, 2051, 3459, 4352
Tsp45I GTSAC 7 cut(s) 319, 652, 917, 991, 2051, 3459, 4352
TspGWI ACGGA 7 cut(s) 83, 211, 288, 384, 1310, 2747, 3733
TspMI CCCGGG 1 cut(s) 648
TspRI CASTG 4 cut(s) 355, 922, 2056, 3468
VpaK11BI GGWCC 8 cut(s) 211, 645, 790, 1435, 1824, 2337, 2684, 4070
VspI ATTAAT 1 cut(s) 2307
XapI RAATTY 6 cut(s) 527, 677, 2783, 2874, 3024, 3056
XbaI TCTAGA 1 cut(s) 1891
XceI RCATGY 1 cut(s) 2974
XcmI CCANNNNNNNNNTGG 4 cut(s) 2631, 2760, 3462, 3682
XmaI CCCGGG 1 cut(s) 648
XmnI GAANNNNTTC 4 cut(s) 1233, 1910, 3024, 3215
XspI CTAG 5 cut(s) 441, 1892, 2879, 2952, 4305
ZraI GACGTC 1 cut(s) 331
ZrmI AGTACT 3 cut(s) 1858, 2263, 2866
Zsp2I ATGCAT 1 cut(s) 2976
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.