Rmu_sc0011424.1_g000020

RNA helicase SDE3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0011424.1
OG0000595
Physical Location & Seq
Forward (+)
68334 .. 72236
3903 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0011424.1_g000020.1.cds

Sequence Viewer

Length: 2559 bp
atgggttttaattggcttaacaggttggggataatcattggggcttcagcaatattattgtgggaatactttgaaaataagagacccacaccatcatcatcatcccctgaaacgccacctcagcggcagccaacgtcatctaattctaaaccacatccagcctccgcagaacccccaccatattattctaaaccatctccatctccatcttccccgaaacccccaccatattcttctaaaccaccttcatctccagcttcttctaaaccacctacatccccagtttcttctaaaccaccttcatccccagcttcttctcccccaccatattcttctaaaccaccttcatccccagcttcttctaaaccaccttcatctgcagcttccccacaacccccagcatattcttctaaaccatcttcatctccatcttccccaaaacacccaccagcacatttttctataccatctccatctccacctccatctcccccaaagtgccctcctgtctttaagtcagttctacgcccagcctcctcttcctcaaataatgatgatataagtcaaaaccaacaatatgggaagagaacagcttatgtgtgtgttgaagaggatccattacctgtattcatgattcctgaggatgtcgaagccttgatcaagaaagaaattgcgcccaaagttctgaatcgcccattatctcccacaacatacaaggactactttgccgctctcttatattctgaagatttctacttggagaaatggagtgatttccttttgaagggtgtgacattagcgttggatgaaagttcaatttataaaaaccggaaaaaggaagcactttataaaaatcggaaaaaggaagataaggaagacaaaacctttgtagcatttgagcttgatcgtgttcctgaggaacgaccattcctcttgtcaagggacttggtccttgcacgaccagtggggaagagttctactgagccctttaagggccccatccataaagttgttgggagcaatcgcgtattggttgaatttgaggatggcttttattcttatcatcattccaacaaaaaatatgatatcagcttctcatacaatagggtttgtttgaaaagagcccaccatgcagtaaaagctgcatcagatgccttgtttcagaacttcctcttccctgactgtgactcacgagcaagcattcctgctgcaccagctctgctttctacaacctatcataagcttgatcaagagcaacgttctgcagtcggacatatcttaaggattcagggctcaccaccttatgtagtaactggtccagactgtgaaggcaaatcttttgcaaatttaagagaaccttcaagaactggagtggttgttagtgaagcagtaaaccaattatgtcggaagtcaccagagtatcgcattcttatctgtgcgcctactaacagctgctgcgatatgctgatgagaagcttggtgaaggtgattccagagactactatgtttcgtgccaatgctgcattccgagacagagatgaggtacctgaggacatcctccgttcatccctttacaaagagtcttgtttttcttgtcctgcaatagagaaactccgggaatacaaggtgattttctcaactttcatgagtagcttccgactacatgataaaggcatagctgctggacattttagccatatttttctggtggatgcttcatctgctattgagccggaaacattggtcgccctgactaactttgctgtcaaggccactagtgttatagttactggtaatcatggaaatagtccgctctgggttcgcgctgacattgcaaggaaaaagggactcaagatttcatactttgaacgactctgcaagcgtaggccatacagaagccccagtccaatgttcatcacacaactagacctgaaccaaaagtcagacctcggtcgtttacctcttgtgactgaaggtatggcctcgggcttccttcacttgatgatgggcctccaagctaccttgtttggtgaagttgatagtctcgtctcggacttccttcacctgatgttgggcttccaagctcctttcttgggtggaactaatggcctcatgtcgggcttcctccacctgatgttgggcttccaagctcctttcttgggtggaactgatggcctcacgtcaggcttcctccacctgatgttgagctccttgttggccgatagtcttgtcacggacttccttcacctgatgttgggcttcctccacctcgagttggcggagccctatggaaaggactatgcattttggcatagctttcggagagaagagaaagttgaagtcccccttcagccttatgaaggctggagttggtacgtgacagatgtcaccaccatgtggtccgatatggttggtgaatggtcagggacaggtgtcgcctatccaagtttcccttttcctggaattactcttaggccatgccttatgtcagaacttgacacgtggcatgaggtcgaggcttctaagcttgggggtcgaactcttggtcttggggattga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

852

Amino Acids

94.24

Weight (kDa)

8.54

Isoelectric Point (pI)

62.43

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000595)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g37100 FvH4_4g37100 FvH4_4g37100
malus_domestica MD13G1238300.v1.1 MD16G1243100.v1.1 MD16G1243200.v1.1
prunus_persica Prupe.1G070100_v2.0.a1 Prupe.1G070200_v2.0.a1 Prupe.1G070300_v2.0.a1 Prupe.1G070300_v2.0.a1
pyrus_communis pycom13g21070 pycom16g20390 pycom16g20400
rosa_chinensis RchiOBHm_Chr1g0318071 RchiOBHm_Chr1g0318111 RchiOBHm_Chr4g0446861 RchiOBHm_Chr4g0446881 RchiOBHm_Chr4g0446891
rosa_laevigata RLG00000005623 RLG00000005624 RLG00000009337 RLG00000030560 RLG00000030566
rosa_multiflora Rmu_co8177450.1_g000001 Rmu_sc0001753.1_g000012 Rmu_sc0001753.1_g000013 Rmu_sc0001753.1_g000014 Rmu_sc0001753.1_g000015 Rmu_sc0011424.1_g000009 Rmu_sc0011424.1_g000020 Rmu_sc0034555.1_g000001 Rmu_ssc0000008.1_g000002
rosa_roxburghii Rroxscaffold_4G00329530 Rroxscaffold_4G00329590 Rroxscaffold_4G00329800 Rroxscaffold_4G00329810 Rroxscaffold_4G00329860 Rroxscaffold_4G00329910 Rroxscaffold_4G00330030 Rroxscaffold_5G00343610 Rroxscaffold_5G00348280 Rroxscaffold_5G00387220 Rroxscaffold_5G00387230
rosa_rugosa Rorug01G0019100 Rorug01G0019100 Rorug01G0019500 Rorug01G0019600 Rorug04G0010100 Rorug04G0375200 Rorug04G0375300 Rorug04G0375400 Rorug04G0375500 Rorug04G0375600 Rorug04G0375700
rosa_samantha Rh1AG030700 Rh1AG030900 Rh1BG026900 Rh1CG029500 Rh1CG029700 Rh1DG040500 Rh4AG088100 Rh4AG436700 Rh4AG436800 Rh4AG436900 Rh4BG084700 Rh4BG445800 Rh4BG445900 Rh4BG446100 Rh4CG096300 Rh4CG463100 Rh4CG463200 Rh4CG463400 Rh4DG444500 Rh4DG444600 Rh4DG444700
rosa_wichuraiana Rw1G002420 Rw1G002440 Rw1G002480 Rw4G037360 Rw4G037370 Rw4G037380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 822, 849
Acc65I GGTACC 1 cut(s) 1543
AccB1I GGYRCC 1 cut(s) 1543
AccB7I CCANNNNNTGG 1 cut(s) 2397
AccBSI CCGCTC 2 cut(s) 731, 1813
AccII CGCG 2 cut(s) 1026, 1824
AciI CCGC 5 cut(s) 124, 165, 729, 1811, 2279
AclI AACGTT 1 cut(s) 1258
AclWI GGATC 2 cut(s) 608, 621
AcoI YGGCCR 1 cut(s) 2217
AcsI RAATTY 2 cut(s) 1037, 1345
AcuI CTGAAG 4 cut(s) 30, 765, 1992, 2333
AcvI CACGTG 1 cut(s) 2502
AdeI CACNNNGTG 1 cut(s) 2397
AfaI GTAC 2 cut(s) 1545, 2375
AflII CTTAAG 1 cut(s) 1279
AflIII ACRYGT 1 cut(s) 2499
AgsI TTSAA 9 cut(s) 74, 608, 784, 816, 1037, 1117, 1362, 1868, 2339
AhlI ACTAGT 1 cut(s) 1775
AjiI CACGTC 1 cut(s) 2181
AjnI CCWGG 1 cut(s) 2458
AjuI GAANNNNNNNTTGG 2 cut(s) 1508, 1540
AloI GAACNNNNNNTCC 2 cut(s) 912, 944
Alw21I GWGCWC 1 cut(s) 2210
Alw26I GTCTC 5 cut(s) 76, 1490, 1524, 2048, 2053
AlwI GGATC 2 cut(s) 608, 621
AlwNI CAGNNNCTG 1 cut(s) 1455
Ama87I CYCGRG 2 cut(s) 1984, 2270
AoxI GGCC 9 cut(s) 994, 1770, 1886, 1980, 2008, 2107, 2173, 2217, 2474
ApaI GGGCCC 1 cut(s) 998
ApeKI GCWGC 8 cut(s) 127, 380, 1142, 1208, 1452, 1455, 1520, 1679
ApoI RAATTY 2 cut(s) 1037, 1345
ArsI GACNNNNNNTTYG 3 cut(s) 869, 901, 2530
Asp718I GGTACC 1 cut(s) 1543
AspLEI GCGC 3 cut(s) 676, 1441, 1826
AspS9I GGNCC 6 cut(s) 949, 994, 995, 1316, 2008, 2400
AsuC2I CCSGG 1 cut(s) 1616
AvaI CYCGRG 2 cut(s) 1984, 2270
AvaII GGWCC 3 cut(s) 949, 1316, 2400
AxyI CCTNAGG 3 cut(s) 639, 915, 1548
BaeGI GKGCMC 2 cut(s) 503, 998
BaeI ACNNNNGTAYC 2 cut(s) 1403, 1436
BamHI GGATCC 1 cut(s) 613
BanI GGYRCC 1 cut(s) 1543
BanII GRGCYC 6 cut(s) 987, 998, 1126, 1295, 2210, 2286
BauI CACGAG 1 cut(s) 1191
BbrPI CACGTG 1 cut(s) 2502
BbsI GAAGAC 1 cut(s) 882
Bbv12I GWGCWC 1 cut(s) 2210
BbvCI CCTCAGC 1 cut(s) 120
BbvI GCAGC 8 cut(s) 139, 392, 1129, 1195, 1439, 1442, 1507, 1666
BcgI CGANNNNNNTGC 2 cut(s) 2300, 2334
BciT130I CCWGG 1 cut(s) 2460
BclI TGATCA 2 cut(s) 657, 1246
BcnI CCSGG 1 cut(s) 1616
BcoDI GTCTC 5 cut(s) 76, 1490, 1524, 2048, 2053
BcuI ACTAGT 1 cut(s) 1775
BfaI CTAG 2 cut(s) 1776, 1925
BfmI CTRYAG 2 cut(s) 378, 1263
BfrI CTTAAG 1 cut(s) 1279
Bme1390I CCNGG 2 cut(s) 1616, 2460
Bme18I GGWCC 3 cut(s) 949, 1316, 2400
BmeT110I CYCGRG 2 cut(s) 1984, 2270
BmgBI CACGTC 1 cut(s) 2181
BmgT120I GGNCC 6 cut(s) 949, 994, 995, 1316, 2008, 2400
BmiI GGNNCC 5 cut(s) 615, 996, 997, 1545, 2283
BmrFI CCNGG 2 cut(s) 1616, 2460
BmrI ACTGGG 2 cut(s) 275, 1896
BmsI GCATC 3 cut(s) 1141, 1154, 1702
BmuI ACTGGG 2 cut(s) 275, 1896
BoxI GACNNNNGTC 3 cut(s) 1950, 2384, 2432
BpiI GAAGAC 1 cut(s) 882
BpmI CTGGAG 3 cut(s) 237, 1389, 2386
Bpu10I CCTNAGC 1 cut(s) 120
BpuEI CTTGAG 1 cut(s) 1835
BpuMI CCSGG 1 cut(s) 1616
BsaAI YACGTR 2 cut(s) 2377, 2502
BsaI GGTCTC 1 cut(s) 76
BsaJI CCNNGG 2 cut(s) 1948, 1983
BsaWI WCCGGW 1 cut(s) 828
BsaXI ACNNNNNCTCC 2 cut(s) 1362, 1392
Bse1I ACTGG 6 cut(s) 281, 962, 1318, 1372, 1795, 1902
Bse21I CCTNAGG 3 cut(s) 639, 915, 1548
Bse3DI GCAATG 1 cut(s) 1830
BseBI CCWGG 1 cut(s) 2460
BseDI CCNNGG 2 cut(s) 1948, 1983
BseMI GCAATG 1 cut(s) 1830
BseMII CTCAG 5 cut(s) 134, 630, 906, 972, 1539
BseNI ACTGG 6 cut(s) 281, 962, 1318, 1372, 1795, 1902
BseRI GAGGAG 1 cut(s) 526
BseSI GKGCMC 2 cut(s) 503, 998
BseXI GCAGC 8 cut(s) 139, 392, 1129, 1195, 1439, 1442, 1507, 1666
BseYI CCCAGC 4 cut(s) 307, 352, 397, 529
BsgI GTGCAG 1 cut(s) 1194
Bsh1236I CGCG 2 cut(s) 1026, 1824
Bsh1285I CGRYCG 1 cut(s) 1954
BshFI GGCC 9 cut(s) 996, 1772, 1888, 1982, 2010, 2109, 2175, 2219, 2476
BshNI GGYRCC 1 cut(s) 1543
BsiEI CGRYCG 1 cut(s) 1954
BsiHKAI GWGCWC 1 cut(s) 2210
BsiHKCI CYCGRG 2 cut(s) 1984, 2270
BsiSI CCGG 3 cut(s) 829, 1615, 1733
BslFI GGGAC 4 cut(s) 956, 1860, 2327, 2440
BsmAI GTCTC 5 cut(s) 76, 1490, 1524, 2048, 2053
BsmBI CGTCTC 1 cut(s) 2053
BsmFI GGGAC 4 cut(s) 956, 1860, 2327, 2440
BsmI GAATGC 3 cut(s) 1200, 1425, 1523
BsnI GGCC 9 cut(s) 996, 1772, 1888, 1982, 2010, 2109, 2175, 2219, 2476
Bso31I GGTCTC 1 cut(s) 76
BsoBI CYCGRG 2 cut(s) 1984, 2270
Bsp120I GGGCCC 1 cut(s) 994
Bsp1286I GDGCHC 7 cut(s) 503, 987, 998, 1126, 1295, 2210, 2286
Bsp143I GATC 4 cut(s) 613, 657, 904, 1246
BspACI CCGC 5 cut(s) 124, 165, 729, 1811, 2279
BspANI GGCC 9 cut(s) 996, 1772, 1888, 1982, 2010, 2109, 2175, 2219, 2476
BspCNI CTCAG 5 cut(s) 133, 631, 907, 973, 1540
BspFNI CGCG 2 cut(s) 1026, 1824
BspHI TCATGA 2 cut(s) 630, 1644
BspLI GGNNCC 5 cut(s) 615, 996, 997, 1545, 2283
BspMAI CTGCAG 2 cut(s) 382, 1267
BspPI GGATC 2 cut(s) 608, 621
BspT107I GGYRCC 1 cut(s) 1543
BspTI CTTAAG 1 cut(s) 1279
BspTNI GGTCTC 1 cut(s) 76
BsrBI CCGCTC 2 cut(s) 731, 1813
BsrDI GCAATG 1 cut(s) 1830
BsrI ACTGG 6 cut(s) 281, 962, 1318, 1372, 1795, 1902
BssECI CCNNGG 2 cut(s) 1948, 1983
BssMI GATC 4 cut(s) 613, 657, 904, 1246
BssSI CACGAG 1 cut(s) 1191
Bst2BI CACGAG 1 cut(s) 1191
Bst2UI CCWGG 1 cut(s) 2460
Bst4CI ACNGT 2 cut(s) 1184, 1325
Bst6I CTCTTC 6 cut(s) 544, 578, 603, 965, 1178, 2322
BstAFI CTTAAG 1 cut(s) 1279
BstAPI GCANNNNNTGC 1 cut(s) 1151
BstBAI YACGTR 2 cut(s) 2377, 2502
BstC8I GCNNGC 2 cut(s) 1198, 1880
BstDEI CTNAG 7 cut(s) 120, 639, 915, 981, 1548, 2471, 2523
BstENI CCTNNNNNAGG 2 cut(s) 782, 2358
BstFNI CGCG 2 cut(s) 1026, 1824
BstHHI GCGC 3 cut(s) 676, 1441, 1826
BstKTI GATC 4 cut(s) 616, 660, 907, 1249
BstMAI GTCTC 5 cut(s) 76, 1490, 1524, 2048, 2053
BstMBI GATC 4 cut(s) 613, 657, 904, 1246
BstMCI CGRYCG 1 cut(s) 1954
BstMWI GCNNNNNNNGC 9 cut(s) 121, 1130, 1139, 1151, 1214, 1520, 1721, 1769, 1832
BstNI CCWGG 1 cut(s) 2460
BstPAI GACNNNNGTC 3 cut(s) 1950, 2384, 2432
BstSCI CCNGG 2 cut(s) 1614, 2458
BstSFI CTRYAG 2 cut(s) 378, 1263
BstSLI GKGCMC 2 cut(s) 503, 998
BstUI CGCG 2 cut(s) 1026, 1824
BstV1I GCAGC 8 cut(s) 139, 392, 1129, 1195, 1439, 1442, 1507, 1666
BstV2I GAAGAC 1 cut(s) 882
BstX2I RGATCY 1 cut(s) 613
BstXI CCANNNNNNTGG 1 cut(s) 578
BstYI RGATCY 1 cut(s) 613
Bsu36I CCTNAGG 3 cut(s) 639, 915, 1548
BsuRI GGCC 9 cut(s) 996, 1772, 1888, 1982, 2010, 2109, 2175, 2219, 2476
BtrI CACGTC 1 cut(s) 2181
BtsIMutI CAGTG 1 cut(s) 969
Cac8I GCNNGC 2 cut(s) 1198, 1880
CaiI CAGNNNCTG 1 cut(s) 1455
CciI TCATGA 2 cut(s) 630, 1644
CfoI GCGC 3 cut(s) 676, 1441, 1826
Cfr13I GGNCC 6 cut(s) 949, 994, 995, 1316, 2008, 2400
Csp6I GTAC 2 cut(s) 1544, 2374
CviAII CATG 9 cut(s) 631, 1130, 1645, 1664, 1799, 2113, 2395, 2478, 2507
CviQI GTAC 2 cut(s) 1544, 2374
DdeI CTNAG 7 cut(s) 120, 639, 915, 981, 1548, 2471, 2523
DpnI GATC 4 cut(s) 615, 659, 906, 1248
DpnII GATC 4 cut(s) 613, 657, 904, 1246
DraIII CACNNNGTG 1 cut(s) 2397
EaeI YGGCCR 1 cut(s) 2217
Eam1104I CTCTTC 6 cut(s) 544, 578, 603, 965, 1178, 2322
EarI CTCTTC 6 cut(s) 544, 578, 603, 965, 1178, 2322
EciI GGCGGA 1 cut(s) 2294
Ecl136II GAGCTC 1 cut(s) 2208
Eco24I GRGCYC 6 cut(s) 987, 998, 1126, 1295, 2210, 2286
Eco31I GGTCTC 1 cut(s) 76
Eco32I GATATC 1 cut(s) 1087
Eco47I GGWCC 3 cut(s) 949, 1316, 2400
Eco53kI GAGCTC 1 cut(s) 2208
Eco57I CTGAAG 4 cut(s) 30, 765, 1992, 2333
Eco72I CACGTG 1 cut(s) 2502
Eco81I CCTNAGG 3 cut(s) 639, 915, 1548
Eco88I CYCGRG 2 cut(s) 1984, 2270
EcoICRI GAGCTC 1 cut(s) 2208
EcoNI CCTNNNNNAGG 2 cut(s) 782, 2358
EcoO109I RGGNCCY 2 cut(s) 994, 995
EcoRII CCWGG 1 cut(s) 2458
EcoRV GATATC 1 cut(s) 1087
EcoT22I ATGCAT 1 cut(s) 2305
EcoT38I GRGCYC 6 cut(s) 987, 998, 1126, 1295, 2210, 2286
Esp3I CGTCTC 1 cut(s) 2053
FaeI CATG 9 cut(s) 634, 1133, 1648, 1667, 1802, 2116, 2398, 2481, 2510
FalI AAGNNNNNCTT 8 cut(s) 707, 739, 1342, 1374, 2331, 2363, 2437, 2469
FaqI GGGAC 4 cut(s) 956, 1860, 2327, 2440
FatI CATG 9 cut(s) 630, 1129, 1644, 1663, 1798, 2112, 2394, 2477, 2506
FbaI TGATCA 2 cut(s) 657, 1246
FriOI GRGCYC 6 cut(s) 987, 998, 1126, 1295, 2210, 2286
FspBI CTAG 2 cut(s) 1776, 1925
GlaI GCGC 3 cut(s) 675, 1440, 1825
GsaI CCCAGC 4 cut(s) 311, 356, 401, 533
GsuI CTGGAG 3 cut(s) 237, 1389, 2386
HaeIII GGCC 9 cut(s) 996, 1772, 1888, 1982, 2010, 2109, 2175, 2219, 2476
HapII CCGG 3 cut(s) 829, 1615, 1733
HhaI GCGC 3 cut(s) 676, 1441, 1826
Hin1II CATG 9 cut(s) 634, 1133, 1648, 1667, 1802, 2116, 2398, 2481, 2510
Hin6I GCGC 3 cut(s) 674, 1439, 1824
HinP1I GCGC 3 cut(s) 674, 1439, 1824
HindIII AAGCTT 3 cut(s) 1241, 1474, 2525
HinfI GANTC 8 cut(s) 634, 688, 1187, 1285, 1489, 1580, 1848, 1872
HpaII CCGG 3 cut(s) 829, 1615, 1733
Hpy166II GTNNAC 2 cut(s) 1393, 1958
Hpy8I GTNNAC 2 cut(s) 1393, 1958
HpyCH4III ACNGT 2 cut(s) 1184, 1325
HpyCH4IV ACGT 5 cut(s) 134, 1258, 2180, 2376, 2501
HpyF10VI GCNNNNNNNGC 9 cut(s) 121, 1130, 1139, 1151, 1214, 1520, 1721, 1769, 1832
HpyF3I CTNAG 7 cut(s) 120, 639, 915, 981, 1548, 2471, 2523
HpySE526I ACGT 5 cut(s) 134, 1258, 2180, 2376, 2501
Hsp92II CATG 9 cut(s) 634, 1133, 1648, 1667, 1802, 2116, 2398, 2481, 2510
HspAI GCGC 3 cut(s) 674, 1439, 1824
KpnI GGTACC 1 cut(s) 1547
Ksp22I TGATCA 2 cut(s) 657, 1246
Kzo9I GATC 4 cut(s) 613, 657, 904, 1246
LmnI GCTCC 5 cut(s) 1017, 2089, 2155, 2213, 2281
Lsp1109I GCAGC 8 cut(s) 139, 392, 1129, 1195, 1439, 1442, 1507, 1666
LweI GCATC 3 cut(s) 1141, 1154, 1702
MaeI CTAG 2 cut(s) 1776, 1925
MaeII ACGT 5 cut(s) 134, 1258, 2180, 2376, 2501
MaeIII GTNAC 9 cut(s) 790, 1184, 1309, 1410, 1786, 1966, 2230, 2377, 2386
MalI GATC 4 cut(s) 615, 659, 906, 1248
MbiI CCGCTC 2 cut(s) 731, 1813
MboI GATC 4 cut(s) 613, 657, 904, 1246
MflI RGATCY 1 cut(s) 613
MhlI GDGCHC 7 cut(s) 503, 987, 998, 1126, 1295, 2210, 2286
MluCI AATT 8 cut(s) 10, 142, 669, 816, 1037, 1345, 1397, 2463
MlyI GAGTC 4 cut(s) 1181, 1589, 1842, 1866
MmeI TCCRAC 5 cut(s) 783, 1095, 1249, 1385, 1681
Mph1103I ATGCAT 1 cut(s) 2305
MseI TTAA 6 cut(s) 9, 18, 513, 990, 1280, 1349
MslI CAYNNNNRTG 1 cut(s) 2393
MspA1I CMGCKG 2 cut(s) 124, 1452
MspCI CTTAAG 1 cut(s) 1279
MspI CCGG 3 cut(s) 829, 1615, 1733
MspR9I CCNGG 2 cut(s) 1616, 2460
Mva1269I GAATGC 3 cut(s) 1200, 1425, 1523
MvaI CCWGG 1 cut(s) 2460
MvnI CGCG 2 cut(s) 1026, 1824
MwoI GCNNNNNNNGC 9 cut(s) 121, 1130, 1139, 1151, 1214, 1520, 1721, 1769, 1832
NciI CCSGG 1 cut(s) 1616
NdeII GATC 4 cut(s) 613, 657, 904, 1246
NlaIII CATG 9 cut(s) 634, 1133, 1648, 1667, 1802, 2116, 2398, 2481, 2510
NlaIV GGNNCC 5 cut(s) 615, 996, 997, 1545, 2283
NmuCI GTSAC 7 cut(s) 790, 1184, 1410, 1966, 2230, 2377, 2386
NsiI ATGCAT 1 cut(s) 2305
PaeR7I CTCGAG 1 cut(s) 2270
PagI TCATGA 2 cut(s) 630, 1644
PctI GAATGC 3 cut(s) 1200, 1425, 1523
PfeI GAWTC 4 cut(s) 634, 688, 1285, 1489
PflFI GACNNNGTC 1 cut(s) 947
PflMI CCANNNNNTGG 1 cut(s) 2397
PfoI TCCNGGA 2 cut(s) 1614, 2458
PleI GAGTC 4 cut(s) 1181, 1588, 1842, 1866
PmaCI CACGTG 1 cut(s) 2502
PmlI CACGTG 1 cut(s) 2502
PpsI GAGTC 4 cut(s) 1181, 1588, 1842, 1866
Ppu21I YACGTR 2 cut(s) 2377, 2502
PshAI GACNNNNGTC 3 cut(s) 1950, 2384, 2432
PsiI TTATAA 2 cut(s) 822, 849
Psp124BI GAGCTC 1 cut(s) 2210
Psp1406I AACGTT 1 cut(s) 1258
Psp6I CCWGG 1 cut(s) 2458
PspCI CACGTG 1 cut(s) 2502
PspFI CCCAGC 4 cut(s) 307, 352, 397, 529
PspGI CCWGG 1 cut(s) 2458
PspN4I GGNNCC 5 cut(s) 615, 996, 997, 1545, 2283
PspOMI GGGCCC 1 cut(s) 994
PspPI GGNCC 6 cut(s) 949, 994, 995, 1316, 2008, 2400
PspXI VCTCGAGB 1 cut(s) 2270
PstI CTGCAG 2 cut(s) 382, 1267
PstNI CAGNNNCTG 1 cut(s) 1455
PsuI RGATCY 1 cut(s) 613
PsyI GACNNNGTC 1 cut(s) 947
PvuII CAGCTG 1 cut(s) 1452
RsaI GTAC 2 cut(s) 1545, 2375
RsaNI GTAC 2 cut(s) 1544, 2374
RseI CAYNNNNRTG 1 cut(s) 2393
SacI GAGCTC 1 cut(s) 2210
SaqAI TTAA 6 cut(s) 9, 18, 513, 990, 1280, 1349
Sau3AI GATC 4 cut(s) 613, 657, 904, 1246
Sau96I GGNCC 6 cut(s) 949, 994, 995, 1316, 2008, 2400
SchI GAGTC 4 cut(s) 1181, 1589, 1842, 1866
ScrFI CCNGG 2 cut(s) 1616, 2460
SduI GDGCHC 7 cut(s) 503, 987, 998, 1126, 1295, 2210, 2286
SfaNI GCATC 3 cut(s) 1141, 1154, 1702
SfcI CTRYAG 2 cut(s) 378, 1263
Sfr274I CTCGAG 1 cut(s) 2270
SinI GGWCC 3 cut(s) 949, 1316, 2400
SlaI CTCGAG 1 cut(s) 2270
SmiMI CAYNNNNRTG 1 cut(s) 2393
SmlI CTYRAG 3 cut(s) 1279, 1850, 2270
SmoI CTYRAG 3 cut(s) 1279, 1850, 2270
SpeI ACTAGT 1 cut(s) 1775
Sse9I AATT 8 cut(s) 10, 142, 669, 816, 1037, 1345, 1397, 2463
SsiI CCGC 5 cut(s) 124, 165, 729, 1811, 2279
SspI AATATT 1 cut(s) 54
SspMI CTAG 2 cut(s) 1776, 1925
SstI GAGCTC 1 cut(s) 2210
StyD4I CCNGG 2 cut(s) 1614, 2458
TaaI ACNGT 2 cut(s) 1184, 1325
TaiI ACGT 5 cut(s) 137, 1261, 2183, 2379, 2504
TaqI TCGA 4 cut(s) 648, 2271, 2514, 2536
TaqII GACCGA 1 cut(s) 1940
TasI AATT 8 cut(s) 10, 142, 669, 816, 1037, 1345, 1397, 2463
TauI GCSGC 2 cut(s) 127, 731
TfiI GAWTC 4 cut(s) 634, 688, 1285, 1489
Tru1I TTAA 6 cut(s) 9, 18, 513, 990, 1280, 1349
Tru9I TTAA 6 cut(s) 9, 18, 513, 990, 1280, 1349
TscAI CASTG 1 cut(s) 969
TseFI GTSAC 7 cut(s) 790, 1184, 1410, 1966, 2230, 2377, 2386
TseI GCWGC 8 cut(s) 127, 380, 1142, 1208, 1452, 1455, 1520, 1679
Tsp45I GTSAC 7 cut(s) 790, 1184, 1410, 1966, 2230, 2377, 2386
TspGWI ACGGA 2 cut(s) 1550, 2249
TspRI CASTG 1 cut(s) 969
Tth111I GACNNNGTC 1 cut(s) 947
Van91I CCANNNNNTGG 1 cut(s) 2397
Vha464I CTTAAG 1 cut(s) 1279
VpaK11BI GGWCC 3 cut(s) 949, 1316, 2400
XagI CCTNNNNNAGG 2 cut(s) 782, 2358
XapI RAATTY 2 cut(s) 1037, 1345
XcmI CCANNNNNNNNNTGG 1 cut(s) 1010
XhoI CTCGAG 1 cut(s) 2270
XspI CTAG 2 cut(s) 1776, 1925
Zsp2I ATGCAT 1 cut(s) 2305
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.