Rh4CG463200

RNA helicase SDE3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0000595
Physical Location & Seq
Reverse (-)
69371132 .. 69373704
2573 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG463200.1

Sequence Viewer

Length: 2103 bp
ATGAGGTATCGGTTGCTGCTGGTCATCGGGAGTTCAGCAATATTATGGTGGACATACAAGAATTGCAAACCTCGCACCCCCAGAAACGAAAGGTCTCGCTTTATACCATTTTCATCATCCCCTCAGCCTCGTCCTGATGGGGTTTGGGCCGTTTGGCTGCTTAATACGTATCAGTTGCTGCTGGTCATCGGGAGTTCAGCAATATTATGGTTGACAAACAAGAATCGCAAACCTCCCCCACCCCCCAGAAACAAATGGTCTCGCTTTAAACCATTTTCATCATCCCCTCAGCCTCGTCCTGATGGGGTTTGGCTTAATCGGTATTGGTTGCTGCTAGTCATCGGGAGTTCAGCAATATTATGGTTGACAAACAAGAATCGCAAACCTCCCCCACCCCCCAGAAACGAAAGTTCTCGCTTCCCAACATATTTTTCTAGACAGACAAATACAGCATATACTTCTAAACCATCTTCATCCTCATCTCCATCTTCCCCAAAACACCCTCCAACATATTTTTCTACACCATCTCCGTCTCCATCTTCCCCGAAACCCCCAGCAAATACTTCTAAACCATCTTCATCCTCATCTCCATCTTCCCCAAAACCCGCCCCCAGCATATATTTTTCTCTATCATCTCCCTCTCCATCTTCCCCAAAGCGCCCTCCTGTCTTTAAGTCAGTTCTATGCCCAGCCTCCTCCTCCTCAAATAATGATGATATAATTCAACAACAACGAGACAGGAACAAAACAGCTTACGTATGTGTTGAAGAGGATTCATTACCTGTATTCACGATTCCTGAGGATATCAAAACCTTGATCAAGAACGAAACTGTGCCCAAAGTTCTCAACTGCCCTTTGTCTCCCACAACGTACAAGGATTACTTTGCTGCTCTGTTATATTCTGAAGATTTCTACTTGGAGAAATGGAGTGATTTCCTTTTGAAGGCTGTGACATTGAAGTTGAATGAAGCTGAAATCTATAAAAATCAGAAAAAGGAAGATAAAGCCTTTGTGGCATTTGAGCTTGATTCTGTTCCCCATAATAGGCCATTCCTCTTATCAAGGGACTTGGTCTTTGCACGACCAGTGGGTAGGAGTACTGCTGAGCCCTTTCAGGGTTTCATTTATCGCATTGTAAGGAGCAAAACCGTTTTAGTTGAATTTGAAGATGCCTTTTATACTCATCATCATTCCAACCAAAAGTATGATGTCAGCTTCTCATTCAATAGAGTTTGTTTGAAAAGAGCTCACCAAGCAGTTAAAGCTGCATCAGAGGCCTTGTTTCAGAACTTCCTCTTCCCTGATTGTGCCTCAAAAGTGAGCATTCCTACTGCACCACCAGCTCTGCTTGCTACTACCCGTCATAAGCTCGATCAATATCAACGTTCTGCAGTTGGACATATCTTAAGGATTCAGGGCTCACCACCTTATGTAGTAACTGGTCCGGACTCTGTACCAGAAGGCAAATGTTTTGCATATTTGAGGGAACCTTCAAGAACTGGAGTGGTTGTTAGTGAAGCAGTATACCAATTATGTCAGAAGTCGCCAGAGTATCGCATTCTTATCTGTGCGCCCACTAACAGCTGCTGCGATGTGCTGATGAGAAGCTTGGGGAAGGTGATTCCAGAGTCGACTATGTTTCGTGCCAATGCTGCATTCCGTGAGAAAGAAGAGGTACCTGAGGATATCCTCCGCTCATCCCTTTACAAAGAGTCCTGTTTTTCTTGTCCTCCAATAGAGAAACTCCGGAAATACAGGGTGATTTTTTCGACTTTCATGAGTAGCTTTCGACTACATGATAAAGGCATAGCTGTTGGACATTTTAGCCATATTTTTCTGGTGGATGCTTCATCTGCTATTGAGCCGGAAACATTGGTCGCCCTGACTAACTTTGCTGACAAGGCTACTAGTGTTATAGTTACTGGTAATCATAGAAATAGCCCACGCTGGGTTCGCTCTGACATTGCAAGGGAAAAGGGACTCAAGATTTCATACTTTGAAAGACTCTGTAAGCTCCGGCCCTATAGAAGCCTCAGTCCAGTGTTCATCACACAATTGGACCTGAACCAAAACTCTGGCTCAGACTACCAAACAGCTTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

700

Amino Acids

79.16

Weight (kDa)

9.65

Isoelectric Point (pI)

62.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MOV-10_beta-barrel PF21634 330 - 406 5.7e-10 Helicase MOV-10, beta-barrel domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000595)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g37100 FvH4_4g37100 FvH4_4g37100
malus_domestica MD13G1238300.v1.1 MD16G1243100.v1.1 MD16G1243200.v1.1
prunus_persica Prupe.1G070100_v2.0.a1 Prupe.1G070200_v2.0.a1 Prupe.1G070300_v2.0.a1 Prupe.1G070300_v2.0.a1
pyrus_communis pycom13g21070 pycom16g20390 pycom16g20400
rosa_chinensis RchiOBHm_Chr1g0318071 RchiOBHm_Chr1g0318111 RchiOBHm_Chr4g0446861 RchiOBHm_Chr4g0446881 RchiOBHm_Chr4g0446891
rosa_laevigata RLG00000005623 RLG00000005624 RLG00000009337 RLG00000030560 RLG00000030566
rosa_multiflora Rmu_co8177450.1_g000001 Rmu_sc0001753.1_g000012 Rmu_sc0001753.1_g000013 Rmu_sc0001753.1_g000014 Rmu_sc0001753.1_g000015 Rmu_sc0011424.1_g000009 Rmu_sc0011424.1_g000020 Rmu_sc0034555.1_g000001 Rmu_ssc0000008.1_g000002
rosa_roxburghii Rroxscaffold_4G00329530 Rroxscaffold_4G00329590 Rroxscaffold_4G00329800 Rroxscaffold_4G00329810 Rroxscaffold_4G00329860 Rroxscaffold_4G00329910 Rroxscaffold_4G00330030 Rroxscaffold_5G00343610 Rroxscaffold_5G00348280 Rroxscaffold_5G00387220 Rroxscaffold_5G00387230
rosa_rugosa Rorug01G0019100 Rorug01G0019100 Rorug01G0019500 Rorug01G0019600 Rorug04G0010100 Rorug04G0375200 Rorug04G0375300 Rorug04G0375400 Rorug04G0375500 Rorug04G0375600 Rorug04G0375700
rosa_samantha Rh1AG030700 Rh1AG030900 Rh1BG026900 Rh1CG029500 Rh1CG029700 Rh1DG040500 Rh4AG088100 Rh4AG436700 Rh4AG436800 Rh4AG436900 Rh4BG084700 Rh4BG445800 Rh4BG445900 Rh4BG446100 Rh4CG096300 Rh4CG463100 Rh4CG463200 Rh4CG463400 Rh4DG444500 Rh4DG444600 Rh4DG444700
rosa_wichuraiana Rw1G002420 Rw1G002440 Rw1G002480 Rw4G037360 Rw4G037370 Rw4G037380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1675
AccB1I GGYRCC 1 cut(s) 1675
AccBSI CCGCTC 1 cut(s) 1695
AccI GTMKAC 2 cut(s) 1524, 1631
AccIII TCCGGA 2 cut(s) 1444, 1746
AciI CCGC 2 cut(s) 606, 1693
AclI AACGTT 1 cut(s) 1384
AcsI RAATTY 1 cut(s) 1160
AcuI CTGAAG 1 cut(s) 924
AfaI GTAC 4 cut(s) 872, 1099, 1455, 1677
AfiI CCNNNNNNNGG 4 cut(s) 943, 1044, 1115, 1948
AflII CTTAAG 1 cut(s) 1405
AhlI ACTAGT 1 cut(s) 1907
AjuI GAANNNNNNNTTGG 6 cut(s) 415, 447, 499, 531, 1640, 1672
Alw21I GWGCWC 1 cut(s) 1249
Alw26I GTCTC 5 cut(s) 99, 264, 537, 729, 864
AlwNI CAGNNNCTG 2 cut(s) 178, 1587
Aor13HI TCCGGA 2 cut(s) 1444, 1746
AoxI GGCC 4 cut(s) 147, 1046, 1275, 2018
ApeKI GCWGC 9 cut(s) 16, 157, 178, 331, 887, 1265, 1584, 1587, 1652
ApoI RAATTY 1 cut(s) 1160
ArsI GACNNNNNNTTYG 2 cut(s) 1058, 1090
Asp718I GGTACC 1 cut(s) 1675
AspLEI GCGC 2 cut(s) 660, 1573
AspS9I GGNCC 4 cut(s) 147, 1442, 2019, 2059
AsuHPI GGTGA 4 cut(s) 1241, 1413, 1630, 1771
AvaII GGWCC 2 cut(s) 1442, 2059
AxyI CCTNAGG 2 cut(s) 798, 1680
BaeGI GKGCMC 1 cut(s) 837
BanI GGYRCC 1 cut(s) 1675
BanII GRGCYC 3 cut(s) 1110, 1249, 1421
Bbv12I GWGCWC 1 cut(s) 1249
BbvCI CCTCAGC 2 cut(s) 123, 288
BbvI GCAGC 9 cut(s) 3, 144, 165, 318, 874, 1252, 1571, 1574, 1639
BccI CCATC 9 cut(s) 131, 296, 475, 493, 532, 544, 580, 598, 652
BceAI ACGGC 1 cut(s) 134
BclI TGATCA 1 cut(s) 816
BcoDI GTCTC 5 cut(s) 99, 264, 537, 729, 864
BcuI ACTAGT 1 cut(s) 1907
BfaI CTAG 3 cut(s) 335, 435, 1908
BfmI CTRYAG 2 cut(s) 1389, 2023
BfoI RGCGCY 1 cut(s) 661
BfrI CTTAAG 1 cut(s) 1405
BglI GCCNNNNNGGC 1 cut(s) 1013
BisI GCNGC 9 cut(s) 17, 158, 179, 332, 888, 1266, 1585, 1588, 1653
BlpI GCTNAGC 1 cut(s) 1104
BlsI GCNGC 9 cut(s) 18, 159, 180, 333, 889, 1267, 1586, 1589, 1654
BmcAI AGTACT 1 cut(s) 1099
Bme18I GGWCC 2 cut(s) 1442, 2059
BmgT120I GGNCC 4 cut(s) 147, 1442, 2019, 2059
BmiI GGNNCC 2 cut(s) 1488, 1677
BmsI GCATC 3 cut(s) 1159, 1277, 1834
BpmI CTGGAG 1 cut(s) 1521
Bpu10I CCTNAGC 2 cut(s) 123, 288
Bpu1102I GCTNAGC 1 cut(s) 1104
BpuEI CTTGAG 1 cut(s) 1967
BsaAI YACGTR 2 cut(s) 168, 757
BsaBI GATNNNNATC 1 cut(s) 1377
BsaI GGTCTC 2 cut(s) 99, 264
BsaWI WCCGGW 2 cut(s) 1444, 1746
BsaXI ACNNNNNCTCC 4 cut(s) 511, 541, 1494, 1524
Bsc4I CCNNNNNNNGG 4 cut(s) 943, 1044, 1115, 1948
Bse1I ACTGG 5 cut(s) 1085, 1444, 1504, 1927, 2039
Bse21I CCTNAGG 2 cut(s) 798, 1680
Bse3DI GCAATG 1 cut(s) 1962
Bse8I GATNNNNATC 1 cut(s) 1377
BseAI TCCGGA 2 cut(s) 1444, 1746
BseGI GGATG 6 cut(s) 116, 281, 473, 578, 1697, 1849
BseJI GATNNNNATC 1 cut(s) 1377
BseLI CCNNNNNNNGG 4 cut(s) 943, 1044, 1115, 1948
BseMI GCAATG 1 cut(s) 1962
BseMII CTCAG 7 cut(s) 137, 302, 789, 1095, 1671, 2047, 2094
BseNI ACTGG 5 cut(s) 1085, 1444, 1504, 1927, 2039
BseRI GAGGAG 3 cut(s) 685, 688, 691
BseSI GKGCMC 1 cut(s) 837
BseXI GCAGC 9 cut(s) 3, 144, 165, 318, 874, 1252, 1571, 1574, 1639
BseYI CCCAGC 4 cut(s) 553, 611, 688, 1947
BsgI GTGCAG 1 cut(s) 1317
BshFI GGCC 4 cut(s) 149, 1048, 1277, 2020
BshNI GGYRCC 1 cut(s) 1675
BsiHKAI GWGCWC 1 cut(s) 1249
BsiSI CCGG 4 cut(s) 1445, 1747, 1865, 2017
BslFI GGGAC 2 cut(s) 1079, 1992
BslI CCNNNNNNNGG 4 cut(s) 943, 1044, 1115, 1948
BsmAI GTCTC 5 cut(s) 99, 264, 537, 729, 864
BsmBI CGTCTC 1 cut(s) 537
BsmFI GGGAC 2 cut(s) 1079, 1992
BsmI GAATGC 3 cut(s) 1323, 1557, 1655
BsnI GGCC 4 cut(s) 149, 1048, 1277, 2020
Bso31I GGTCTC 2 cut(s) 99, 264
Bsp1286I GDGCHC 4 cut(s) 837, 1110, 1249, 1421
Bsp13I TCCGGA 2 cut(s) 1444, 1746
Bsp143I GATC 2 cut(s) 816, 1372
Bsp1720I GCTNAGC 1 cut(s) 1104
BspACI CCGC 2 cut(s) 606, 1693
BspANI GGCC 4 cut(s) 149, 1048, 1277, 2020
BspCNI CTCAG 7 cut(s) 136, 301, 790, 1096, 1672, 2046, 2093
BspEI TCCGGA 2 cut(s) 1444, 1746
BspHI TCATGA 1 cut(s) 1776
BspLI GGNNCC 2 cut(s) 1488, 1677
BspMAI CTGCAG 1 cut(s) 1393
BspT107I GGYRCC 1 cut(s) 1675
BspTI CTTAAG 1 cut(s) 1405
BspTNI GGTCTC 2 cut(s) 99, 264
BsrBI CCGCTC 1 cut(s) 1695
BsrDI GCAATG 1 cut(s) 1962
BsrI ACTGG 5 cut(s) 1085, 1444, 1504, 1927, 2039
BssMI GATC 2 cut(s) 816, 1372
BssNAI GTATAC 1 cut(s) 1525
Bst1107I GTATAC 1 cut(s) 1525
Bst4CI ACNGT 2 cut(s) 832, 1150
Bst6I CTCTTC 3 cut(s) 762, 1301, 1665
BstAFI CTTAAG 1 cut(s) 1405
BstBAI YACGTR 2 cut(s) 168, 757
BstC8I GCNNGC 1 cut(s) 1350
BstDEI CTNAG 7 cut(s) 123, 288, 798, 1104, 1680, 2033, 2080
BstENI CCTNNNNNAGG 1 cut(s) 941
BstF5I GGATG 6 cut(s) 116, 281, 473, 578, 1697, 1849
BstH2I RGCGCY 1 cut(s) 661
BstHHI GCGC 2 cut(s) 660, 1573
BstKTI GATC 2 cut(s) 819, 1375
BstMAI GTCTC 5 cut(s) 99, 264, 537, 729, 864
BstMBI GATC 2 cut(s) 816, 1372
BstSFI CTRYAG 2 cut(s) 1389, 2023
BstSLI GKGCMC 1 cut(s) 837
BstSNI TACGTA 2 cut(s) 168, 757
BstV1I GCAGC 9 cut(s) 3, 144, 165, 318, 874, 1252, 1571, 1574, 1639
BstXI CCANNNNNNTGG 1 cut(s) 2075
BstZ17I GTATAC 1 cut(s) 1525
Bsu36I CCTNAGG 2 cut(s) 798, 1680
BsuRI GGCC 4 cut(s) 149, 1048, 1277, 2020
BtgZI GCGATG 1 cut(s) 1605
BtsCI GGATG 6 cut(s) 116, 281, 473, 578, 1697, 1849
BtsIMutI CAGTG 2 cut(s) 1092, 2046
Cac8I GCNNGC 1 cut(s) 1350
CaiI CAGNNNCTG 2 cut(s) 178, 1587
CciI TCATGA 1 cut(s) 1776
CfoI GCGC 2 cut(s) 660, 1573
Cfr13I GGNCC 4 cut(s) 147, 1442, 2019, 2059
Csp6I GTAC 4 cut(s) 871, 1098, 1454, 1676
CviAII CATG 2 cut(s) 1777, 1796
CviQI GTAC 4 cut(s) 871, 1098, 1454, 1676
DdeI CTNAG 7 cut(s) 123, 288, 798, 1104, 1680, 2033, 2080
DpnI GATC 2 cut(s) 818, 1374
DpnII GATC 2 cut(s) 816, 1372
DraI TTTAAA 1 cut(s) 268
Eam1104I CTCTTC 3 cut(s) 762, 1301, 1665
EarI CTCTTC 3 cut(s) 762, 1301, 1665
Ecl136II GAGCTC 1 cut(s) 1247
Eco105I TACGTA 2 cut(s) 168, 757
Eco147I AGGCCT 1 cut(s) 1277
Eco24I GRGCYC 3 cut(s) 1110, 1249, 1421
Eco31I GGTCTC 2 cut(s) 99, 264
Eco32I GATATC 2 cut(s) 805, 1687
Eco47I GGWCC 2 cut(s) 1442, 2059
Eco53kI GAGCTC 1 cut(s) 1247
Eco57I CTGAAG 1 cut(s) 924
Eco81I CCTNAGG 2 cut(s) 798, 1680
EcoICRI GAGCTC 1 cut(s) 1247
EcoNI CCTNNNNNAGG 1 cut(s) 941
EcoRV GATATC 2 cut(s) 805, 1687
EcoT38I GRGCYC 3 cut(s) 1110, 1249, 1421
Esp3I CGTCTC 1 cut(s) 537
FaeI CATG 2 cut(s) 1780, 1799
FalI AAGNNNNNCTT 2 cut(s) 866, 898
FaqI GGGAC 2 cut(s) 1079, 1992
FatI CATG 2 cut(s) 1776, 1795
FauI CCCGC 1 cut(s) 613
FbaI TGATCA 1 cut(s) 816
FblI GTMKAC 2 cut(s) 1524, 1631
Fnu4HI GCNGC 9 cut(s) 17, 158, 179, 332, 888, 1266, 1585, 1588, 1653
FokI GGATG 6 cut(s) 103, 268, 460, 565, 1684, 1856
FriOI GRGCYC 3 cut(s) 1110, 1249, 1421
Fsp4HI GCNGC 9 cut(s) 17, 158, 179, 332, 888, 1266, 1585, 1588, 1653
FspBI CTAG 3 cut(s) 335, 435, 1908
GlaI GCGC 2 cut(s) 659, 1572
GluI GCNGC 9 cut(s) 17, 158, 179, 332, 888, 1266, 1585, 1588, 1653
GsaI CCCAGC 4 cut(s) 557, 615, 692, 1951
GsuI CTGGAG 1 cut(s) 1521
HaeII RGCGCY 1 cut(s) 661
HaeIII GGCC 4 cut(s) 149, 1048, 1277, 2020
HapII CCGG 4 cut(s) 1445, 1747, 1865, 2017
HhaI GCGC 2 cut(s) 660, 1573
Hin1II CATG 2 cut(s) 1780, 1799
Hin6I GCGC 2 cut(s) 658, 1571
HinP1I GCGC 2 cut(s) 658, 1571
HincII GTYRAC 3 cut(s) 213, 366, 1632
HindII GTYRAC 3 cut(s) 213, 366, 1632
HindIII AAGCTT 1 cut(s) 1606
HpaII CCGG 4 cut(s) 1445, 1747, 1865, 2017
HphI GGTGA 4 cut(s) 1241, 1413, 1630, 1771
Hpy166II GTNNAC 5 cut(s) 51, 213, 366, 1525, 1632
Hpy188I TCNGA 8 cut(s) 904, 990, 1273, 1287, 1539, 1960, 2083, 2102
Hpy8I GTNNAC 5 cut(s) 51, 213, 366, 1525, 1632
HpyAV CCTTC 4 cut(s) 937, 1454, 1500, 1609
HpyCH4III ACNGT 2 cut(s) 832, 1150
HpyCH4IV ACGT 4 cut(s) 167, 756, 869, 1384
HpyCH4V TGCA 8 cut(s) 66, 1079, 1268, 1334, 1391, 1475, 1655, 1967
HpyF3I CTNAG 7 cut(s) 123, 288, 798, 1104, 1680, 2033, 2080
HpySE526I ACGT 4 cut(s) 167, 756, 869, 1384
Hsp92II CATG 2 cut(s) 1780, 1799
HspAI GCGC 2 cut(s) 658, 1571
Kpn2I TCCGGA 2 cut(s) 1444, 1746
KpnI GGTACC 1 cut(s) 1679
Ksp22I TGATCA 1 cut(s) 816
Kzo9I GATC 2 cut(s) 816, 1372
LmnI GCTCC 2 cut(s) 1140, 2019
Lsp1109I GCAGC 9 cut(s) 3, 144, 165, 318, 874, 1252, 1571, 1574, 1639
LweI GCATC 3 cut(s) 1159, 1277, 1834
MaeI CTAG 3 cut(s) 335, 435, 1908
MaeII ACGT 4 cut(s) 167, 756, 869, 1384
MaeIII GTNAC 3 cut(s) 949, 1435, 1918
MalI GATC 2 cut(s) 818, 1374
MbiI CCGCTC 1 cut(s) 1695
MboI GATC 2 cut(s) 816, 1372
MfeI CAATTG 1 cut(s) 2054
MhlI GDGCHC 4 cut(s) 837, 1110, 1249, 1421
MluCI AATT 5 cut(s) 61, 720, 1160, 1529, 2054
MlyI GAGTC 5 cut(s) 1442, 1637, 1721, 1974, 1998
MmeI TCCRAC 4 cut(s) 530, 1218, 1375, 1795
MroI TCCGGA 2 cut(s) 1444, 1746
MseI TTAA 6 cut(s) 162, 267, 315, 672, 1260, 1406
MspA1I CMGCKG 1 cut(s) 1584
MspCI CTTAAG 1 cut(s) 1405
MspI CCGG 4 cut(s) 1445, 1747, 1865, 2017
MunI CAATTG 1 cut(s) 2054
Mva1269I GAATGC 3 cut(s) 1323, 1557, 1655
NdeII GATC 2 cut(s) 816, 1372
NlaIII CATG 2 cut(s) 1780, 1799
NlaIV GGNNCC 2 cut(s) 1488, 1677
NmuCI GTSAC 1 cut(s) 949
PagI TCATGA 1 cut(s) 1776
PceI AGGCCT 1 cut(s) 1277
PctI GAATGC 3 cut(s) 1323, 1557, 1655
PfeI GAWTC 7 cut(s) 223, 376, 773, 793, 1028, 1411, 1621
PflFI GACNNNGTC 1 cut(s) 1070
PkrI GCNGC 9 cut(s) 18, 159, 180, 333, 889, 1267, 1586, 1589, 1654
PleI GAGTC 5 cut(s) 1442, 1636, 1720, 1974, 1998
PpsI GAGTC 5 cut(s) 1442, 1636, 1720, 1974, 1998
Ppu21I YACGTR 2 cut(s) 168, 757
Psp124BI GAGCTC 1 cut(s) 1249
Psp1406I AACGTT 1 cut(s) 1384
PspFI CCCAGC 4 cut(s) 553, 611, 688, 1947
PspN4I GGNNCC 2 cut(s) 1488, 1677
PspPI GGNCC 4 cut(s) 147, 1442, 2019, 2059
PstI CTGCAG 1 cut(s) 1393
PstNI CAGNNNCTG 2 cut(s) 178, 1587
PsyI GACNNNGTC 1 cut(s) 1070
PvuII CAGCTG 1 cut(s) 1584
RsaI GTAC 4 cut(s) 872, 1099, 1455, 1677
RsaNI GTAC 4 cut(s) 871, 1098, 1454, 1676
SacI GAGCTC 1 cut(s) 1249
SalI GTCGAC 1 cut(s) 1630
SaqAI TTAA 6 cut(s) 162, 267, 315, 672, 1260, 1406
SatI GCNGC 9 cut(s) 17, 158, 179, 332, 888, 1266, 1585, 1588, 1653
Sau3AI GATC 2 cut(s) 816, 1372
Sau96I GGNCC 4 cut(s) 147, 1442, 2019, 2059
ScaI AGTACT 1 cut(s) 1099
SchI GAGTC 5 cut(s) 1442, 1637, 1721, 1974, 1998
SduI GDGCHC 4 cut(s) 837, 1110, 1249, 1421
SfaNI GCATC 3 cut(s) 1159, 1277, 1834
SfcI CTRYAG 2 cut(s) 1389, 2023
SinI GGWCC 2 cut(s) 1442, 2059
SmlI CTYRAG 2 cut(s) 1405, 1982
SmoI CTYRAG 2 cut(s) 1405, 1982
SnaBI TACGTA 2 cut(s) 168, 757
SpeI ACTAGT 1 cut(s) 1907
Sse9I AATT 5 cut(s) 61, 720, 1160, 1529, 2054
SseBI AGGCCT 1 cut(s) 1277
SsiI CCGC 2 cut(s) 606, 1693
SspI AATATT 3 cut(s) 42, 204, 357
SspMI CTAG 3 cut(s) 335, 435, 1908
SstI GAGCTC 1 cut(s) 1249
StuI AGGCCT 1 cut(s) 1277
TaaI ACNGT 2 cut(s) 832, 1150
TaiI ACGT 4 cut(s) 170, 759, 872, 1387
TaqI TCGA 4 cut(s) 1371, 1631, 1769, 1789
TasI AATT 5 cut(s) 61, 720, 1160, 1529, 2054
TatI WGTACW 1 cut(s) 1097
TfiI GAWTC 7 cut(s) 223, 376, 773, 793, 1028, 1411, 1621
Tru1I TTAA 6 cut(s) 162, 267, 315, 672, 1260, 1406
Tru9I TTAA 6 cut(s) 162, 267, 315, 672, 1260, 1406
TscAI CASTG 2 cut(s) 1092, 2046
TseFI GTSAC 1 cut(s) 949
TseI GCWGC 9 cut(s) 16, 157, 178, 331, 887, 1265, 1584, 1587, 1652
Tsp45I GTSAC 1 cut(s) 949
TspGWI ACGGA 2 cut(s) 519, 1649
TspRI CASTG 2 cut(s) 1092, 2046
Tth111I GACNNNGTC 1 cut(s) 1070
Vha464I CTTAAG 1 cut(s) 1405
VpaK11BI GGWCC 2 cut(s) 1442, 2059
XagI CCTNNNNNAGG 1 cut(s) 941
XapI RAATTY 1 cut(s) 1160
XbaI TCTAGA 1 cut(s) 434
XmiI GTMKAC 2 cut(s) 1524, 1631
XspI CTAG 3 cut(s) 335, 435, 1908
ZrmI AGTACT 1 cut(s) 1099
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.