Rw1G002420

RNA helicase SDE3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000595
Physical Location & Seq
Reverse (-)
4443690 .. 4446147
2458 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G002420.1

Sequence Viewer

Length: 1764 bp
ATGGGGTCATCTAATTCTAAACCACATCCAGCCTCCGCAGAATCCCCACCATATTATTCTAAACCATCTCCATCTTCCCCGAAACCCCCACCATATTCTTCTAAACCACCTTCATCTCCAGCTTGCCCACAACCCCCACCATATTCTTCTAAACCACCTTCATCCCCAGCTTCTTCTAAACCACCTTCATCTCCAGTTTCCCCACAACCCCCAGCATATTCTTCTAAGCCACCTTCATCCCCAGCATATTCTTCTAAACCACCTTCATCTCCAGTTTCCCCACAACCCCCAGCATATTCTTCTAAACCATCTTCATCTCCATTTTCCCCAAAGTGCCCTCCTGTCTTTAAGTCAGTTCTACGCCCAGCCTCCTCCTCCTCAAATAATGATGATATAAGTCAACACCAACAATATGAGAAGAGAACAGCTTTTGTGTGTCTTGAAAAGGATCCATTACCTGTATTCATGATTCCTGAGGATATCGAAGCCTTGATCAAGAAAGAAATTGCGCCCAAAGTTCTGAATCGCCCATTATCTCCCACAACATACAAGGACTACTTTGCCGCTCTCTTATATTCTGAAGATTTCTACTTGGAGAAATGGAGTGATTTCCTTTTGAAGGGTGTGACATTAGCGTTGGATGAAAGTTCAATTTATAAAAACCGGAAAAAGGAAGCACTTTATAAAAATCGGAAAAAGGAAGATAAGGAAGACAAAACCTTTGTAGCATTTGAGCTTGATTGTGTTCCTGAGAAACGGCCATTCCTCTTATCAAGGGACTTGGTCCTTGCACGACCAGTGGGTAATAGTACTACTGAGCCCTTTAAGGGCCTCATCTATAAAGTTGTTGGGAGCAATCGCGTTTTGGTTGAATTTGAGGATGACTTTTATTCTTATCATCATTCCAACAAAAAATATGATATCAGCTTCTCATTCAATAGAGTTTGTTTGAAAAGAGCCCACCATGCCGTAAAAGCTGCATCAGATGCCTTGTTTCAGAACTTGCTCTTCCCTGACTGTGACTCACGAGCAAGCATTCCTACAGCACCAGCTCTGCTTTCTACTACCTGTCATAAGCTTGATCAAGATCAACGTTCGGCAGTCGGACATATCTTAAGGATTCAGGGCTCACCACCTTATGTAGTAACTGAACCTTCAAGAACTGGAGTGGTTGTTAGTGAAGCAGTATACCAATTATGTCGGAAGTCACCAGAGTATCGCATTCTTATCTGTGCACCTACTAACAGCTGCTGCGATATGCTGATGAGAAGCTTGGTGAAGGTGATTCCAGGGTCGACTATGTTTCGTGCCAATGCTGCATTCCGAGAGAGAGATGAGGTACCTGAGGACATCCTCCGCTCATCCCTTTACAAAGAGTCTTGTTTTTCTTGTCCTGCAATAGAGAAACTCCGGGAATACAAGGTGATTTTCTCAACTTTCGTGAGTAGCTTCCGACTACATGATAAAGGCATAGCTGCTGGACATTTTAGCCATATTTTTCTGGTGGATGCTTCATCTACTATTGAGCCGGAAACATTGGTCGCCCTGACTAACTTCGCTGTCAAGGCCACTACTGTTATAGTTACTGGTAATCATGGAAATAGTCCGCTCTGGGTTCGCGCTGACATTGCAAGGAAAAAGGGACTCAAGATTTCCTACTTTGAACGACTCTGCAAGCTCAGGCCATATAGAAGCCCCAGTCCAATGTTCATCACACAACTAGACCTGAACCAAAAGTCAGAGTACCAAACAAGACCTTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

587

Amino Acids

65.4

Weight (kDa)

9.21

Isoelectric Point (pI)

69.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MOV-10_beta-barrel PF21634 234 - 310 6.5e-10 Helicase MOV-10, beta-barrel domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000595)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g37100 FvH4_4g37100 FvH4_4g37100
malus_domestica MD13G1238300.v1.1 MD16G1243100.v1.1 MD16G1243200.v1.1
prunus_persica Prupe.1G070100_v2.0.a1 Prupe.1G070200_v2.0.a1 Prupe.1G070300_v2.0.a1 Prupe.1G070300_v2.0.a1
pyrus_communis pycom13g21070 pycom16g20390 pycom16g20400
rosa_chinensis RchiOBHm_Chr1g0318071 RchiOBHm_Chr1g0318111 RchiOBHm_Chr4g0446861 RchiOBHm_Chr4g0446881 RchiOBHm_Chr4g0446891
rosa_laevigata RLG00000005623 RLG00000005624 RLG00000009337 RLG00000030560 RLG00000030566
rosa_multiflora Rmu_co8177450.1_g000001 Rmu_sc0001753.1_g000012 Rmu_sc0001753.1_g000013 Rmu_sc0001753.1_g000014 Rmu_sc0001753.1_g000015 Rmu_sc0011424.1_g000009 Rmu_sc0011424.1_g000020 Rmu_sc0034555.1_g000001 Rmu_ssc0000008.1_g000002
rosa_roxburghii Rroxscaffold_4G00329530 Rroxscaffold_4G00329590 Rroxscaffold_4G00329800 Rroxscaffold_4G00329810 Rroxscaffold_4G00329860 Rroxscaffold_4G00329910 Rroxscaffold_4G00330030 Rroxscaffold_5G00343610 Rroxscaffold_5G00348280 Rroxscaffold_5G00387220 Rroxscaffold_5G00387230
rosa_rugosa Rorug01G0019100 Rorug01G0019100 Rorug01G0019500 Rorug01G0019600 Rorug04G0010100 Rorug04G0375200 Rorug04G0375300 Rorug04G0375400 Rorug04G0375500 Rorug04G0375600 Rorug04G0375700
rosa_samantha Rh1AG030700 Rh1AG030900 Rh1BG026900 Rh1CG029500 Rh1CG029700 Rh1DG040500 Rh4AG088100 Rh4AG436700 Rh4AG436800 Rh4AG436900 Rh4BG084700 Rh4BG445800 Rh4BG445900 Rh4BG446100 Rh4CG096300 Rh4CG463100 Rh4CG463200 Rh4CG463400 Rh4DG444500 Rh4DG444600 Rh4DG444700
rosa_wichuraiana Rw1G002420 Rw1G002440 Rw1G002480 Rw4G037360 Rw4G037370 Rw4G037380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 657, 684
Acc65I GGTACC 1 cut(s) 1339
AccB1I GGYRCC 1 cut(s) 1339
AccBSI CCGCTC 3 cut(s) 566, 1359, 1609
AccI GTMKAC 2 cut(s) 1188, 1295
AccII CGCG 2 cut(s) 861, 1620
AciI CCGC 4 cut(s) 36, 564, 1357, 1607
AclI AACGTT 1 cut(s) 1093
AclWI GGATC 2 cut(s) 443, 456
AcoI YGGCCR 1 cut(s) 758
AcsI RAATTY 1 cut(s) 872
AcuI CTGAAG 1 cut(s) 600
AfaI GTAC 3 cut(s) 811, 1341, 1745
AfiI CCNNNNNNNGG 3 cut(s) 619, 670, 827
AflII CTTAAG 1 cut(s) 1114
AgsI TTSAA 8 cut(s) 443, 619, 651, 872, 937, 952, 1158, 1664
AjnI CCWGG 1 cut(s) 1288
AjuI GAANNNNNNNTTGG 2 cut(s) 1304, 1336
Alw21I GWGCWC 1 cut(s) 1237
Alw44I GTGCAC 1 cut(s) 1233
AlwI GGATC 2 cut(s) 443, 456
AlwNI CAGNNNCTG 1 cut(s) 1251
AoxI GGCC 4 cut(s) 758, 829, 1566, 1682
ApaLI GTGCAC 1 cut(s) 1233
ApeKI GCWGC 5 cut(s) 977, 1248, 1251, 1316, 1475
ApoI RAATTY 1 cut(s) 872
ArsI GACNNNNNNTTYG 2 cut(s) 704, 736
Asp718I GGTACC 1 cut(s) 1339
AspLEI GCGC 2 cut(s) 511, 1622
AspS9I GGNCC 2 cut(s) 784, 829
AsuC2I CCSGG 1 cut(s) 1412
AsuHPI GGTGA 5 cut(s) 1122, 1200, 1288, 1294, 1435
AvaII GGWCC 1 cut(s) 784
AxyI CCTNAGG 2 cut(s) 474, 1344
BaeGI GKGCMC 2 cut(s) 338, 1237
BaeI ACNNNNGTAYC 2 cut(s) 1199, 1232
BamHI GGATCC 1 cut(s) 448
BanI GGYRCC 1 cut(s) 1339
BanII GRGCYC 3 cut(s) 822, 961, 1130
BauI CACGAG 1 cut(s) 1026
BbsI GAAGAC 1 cut(s) 717
Bbv12I GWGCWC 1 cut(s) 1237
BbvI GCAGC 5 cut(s) 964, 1235, 1238, 1303, 1462
BccI CCATC 3 cut(s) 73, 79, 316
BceAI ACGGC 2 cut(s) 773, 953
BciT130I CCWGG 1 cut(s) 1290
BclI TGATCA 2 cut(s) 492, 1081
BcnI CCSGG 1 cut(s) 1412
BfaI CTAG 1 cut(s) 1721
BfmI CTRYAG 1 cut(s) 1041
BfrI CTTAAG 1 cut(s) 1114
BisI GCNGC 6 cut(s) 564, 978, 1249, 1252, 1317, 1476
BlsI GCNGC 6 cut(s) 565, 979, 1250, 1253, 1318, 1477
BmcAI AGTACT 1 cut(s) 811
Bme1390I CCNGG 2 cut(s) 1290, 1412
Bme18I GGWCC 1 cut(s) 784
BmgT120I GGNCC 2 cut(s) 784, 829
BmiI GGNNCC 2 cut(s) 450, 1341
BmrFI CCNGG 2 cut(s) 1290, 1412
BmrI ACTGGG 1 cut(s) 1692
BmsI GCATC 3 cut(s) 976, 989, 1498
BmuI ACTGGG 1 cut(s) 1692
BpiI GAAGAC 1 cut(s) 717
BpmI CTGGAG 4 cut(s) 102, 177, 255, 1185
Bpu10I CCTNAGC 1 cut(s) 1679
BpuEI CTTGAG 1 cut(s) 1631
BpuMI CCSGG 1 cut(s) 1412
BsaBI GATNNNNATC 1 cut(s) 1086
BsaJI CCNNGG 1 cut(s) 1289
BsaWI WCCGGW 1 cut(s) 663
BsaXI ACNNNNNCTCC 2 cut(s) 1158, 1188
Bsc4I CCNNNNNNNGG 3 cut(s) 619, 670, 827
Bse1I ACTGG 6 cut(s) 194, 272, 797, 1168, 1591, 1698
Bse21I CCTNAGG 2 cut(s) 474, 1344
Bse3DI GCAATG 1 cut(s) 1626
Bse8I GATNNNNATC 1 cut(s) 1086
BseBI CCWGG 1 cut(s) 1290
BseDI CCNNGG 1 cut(s) 1289
BseGI GGATG 8 cut(s) 25, 161, 236, 646, 886, 1350, 1361, 1513
BseJI GATNNNNATC 1 cut(s) 1086
BseLI CCNNNNNNNGG 3 cut(s) 619, 670, 827
BseMI GCAATG 1 cut(s) 1626
BseMII CTCAG 5 cut(s) 465, 741, 807, 1335, 1693
BseNI ACTGG 6 cut(s) 194, 272, 797, 1168, 1591, 1698
BseRI GAGGAG 3 cut(s) 361, 364, 367
BseSI GKGCMC 2 cut(s) 338, 1237
BseXI GCAGC 5 cut(s) 964, 1235, 1238, 1303, 1462
BseYI CCCAGC 5 cut(s) 166, 211, 241, 289, 364
Bsh1236I CGCG 2 cut(s) 861, 1620
BshFI GGCC 4 cut(s) 760, 831, 1568, 1684
BshNI GGYRCC 1 cut(s) 1339
BsiHKAI GWGCWC 1 cut(s) 1237
BsiSI CCGG 3 cut(s) 664, 1411, 1529
BslFI GGGAC 2 cut(s) 791, 1656
BslI CCNNNNNNNGG 3 cut(s) 619, 670, 827
BsmFI GGGAC 2 cut(s) 791, 1656
BsmI GAATGC 3 cut(s) 1035, 1221, 1319
BsnI GGCC 4 cut(s) 760, 831, 1568, 1684
Bsp1286I GDGCHC 5 cut(s) 338, 822, 961, 1130, 1237
Bsp143I GATC 4 cut(s) 448, 492, 1081, 1087
BspACI CCGC 4 cut(s) 36, 564, 1357, 1607
BspANI GGCC 4 cut(s) 760, 831, 1568, 1684
BspCNI CTCAG 5 cut(s) 466, 742, 808, 1336, 1692
BspFNI CGCG 2 cut(s) 861, 1620
BspHI TCATGA 1 cut(s) 465
BspLI GGNNCC 2 cut(s) 450, 1341
BspPI GGATC 2 cut(s) 443, 456
BspQI GCTCTTC 1 cut(s) 1013
BspT107I GGYRCC 1 cut(s) 1339
BspTI CTTAAG 1 cut(s) 1114
BsrBI CCGCTC 3 cut(s) 566, 1359, 1609
BsrDI GCAATG 1 cut(s) 1626
BsrI ACTGG 6 cut(s) 194, 272, 797, 1168, 1591, 1698
BssECI CCNNGG 1 cut(s) 1289
BssMI GATC 4 cut(s) 448, 492, 1081, 1087
BssNAI GTATAC 1 cut(s) 1189
BssSI CACGAG 1 cut(s) 1026
Bst1107I GTATAC 1 cut(s) 1189
Bst2BI CACGAG 1 cut(s) 1026
Bst2UI CCWGG 1 cut(s) 1290
Bst4CI ACNGT 2 cut(s) 1019, 1576
Bst6I CTCTTC 2 cut(s) 413, 1013
BstAFI CTTAAG 1 cut(s) 1114
BstAPI GCANNNNNTGC 1 cut(s) 986
BstC8I GCNNGC 3 cut(s) 124, 1033, 1676
BstDEI CTNAG 6 cut(s) 225, 474, 750, 816, 1344, 1679
BstENI CCTNNNNNAGG 1 cut(s) 617
BstF5I GGATG 8 cut(s) 25, 161, 236, 646, 886, 1350, 1361, 1513
BstFNI CGCG 2 cut(s) 861, 1620
BstHHI GCGC 2 cut(s) 511, 1622
BstKTI GATC 4 cut(s) 451, 495, 1084, 1090
BstMBI GATC 4 cut(s) 448, 492, 1081, 1087
BstMWI GCNNNNNNNGC 6 cut(s) 965, 974, 986, 1316, 1565, 1628
BstNI CCWGG 1 cut(s) 1290
BstSCI CCNGG 2 cut(s) 1288, 1410
BstSFI CTRYAG 1 cut(s) 1041
BstSLI GKGCMC 2 cut(s) 338, 1237
BstUI CGCG 2 cut(s) 861, 1620
BstV1I GCAGC 5 cut(s) 964, 1235, 1238, 1303, 1462
BstV2I GAAGAC 1 cut(s) 717
BstX2I RGATCY 1 cut(s) 448
BstYI RGATCY 1 cut(s) 448
BstZ17I GTATAC 1 cut(s) 1189
Bsu36I CCTNAGG 2 cut(s) 474, 1344
BsuRI GGCC 4 cut(s) 760, 831, 1568, 1684
BtsCI GGATG 8 cut(s) 25, 161, 236, 646, 886, 1350, 1361, 1513
BtsIMutI CAGTG 1 cut(s) 804
Cac8I GCNNGC 3 cut(s) 124, 1033, 1676
CaiI CAGNNNCTG 1 cut(s) 1251
CciI TCATGA 1 cut(s) 465
CfoI GCGC 2 cut(s) 511, 1622
Cfr13I GGNCC 2 cut(s) 784, 829
Csp6I GTAC 3 cut(s) 810, 1340, 1744
CviAII CATG 4 cut(s) 466, 965, 1460, 1595
CviQI GTAC 3 cut(s) 810, 1340, 1744
DdeI CTNAG 6 cut(s) 225, 474, 750, 816, 1344, 1679
DpnI GATC 4 cut(s) 450, 494, 1083, 1089
DpnII GATC 4 cut(s) 448, 492, 1081, 1087
EaeI YGGCCR 1 cut(s) 758
Eam1104I CTCTTC 2 cut(s) 413, 1013
EarI CTCTTC 2 cut(s) 413, 1013
Eco24I GRGCYC 3 cut(s) 822, 961, 1130
Eco32I GATATC 2 cut(s) 481, 922
Eco47I GGWCC 1 cut(s) 784
Eco57I CTGAAG 1 cut(s) 600
Eco81I CCTNAGG 2 cut(s) 474, 1344
EcoNI CCTNNNNNAGG 1 cut(s) 617
EcoO109I RGGNCCY 1 cut(s) 829
EcoRII CCWGG 1 cut(s) 1288
EcoRV GATATC 2 cut(s) 481, 922
EcoT38I GRGCYC 3 cut(s) 822, 961, 1130
FaeI CATG 4 cut(s) 469, 968, 1463, 1598
FalI AAGNNNNNCTT 2 cut(s) 542, 574
FaqI GGGAC 2 cut(s) 791, 1656
FatI CATG 4 cut(s) 465, 964, 1459, 1594
FbaI TGATCA 2 cut(s) 492, 1081
FblI GTMKAC 2 cut(s) 1188, 1295
Fnu4HI GCNGC 6 cut(s) 564, 978, 1249, 1252, 1317, 1476
FokI GGATG 8 cut(s) 12, 148, 223, 653, 893, 1337, 1348, 1520
FriOI GRGCYC 3 cut(s) 822, 961, 1130
Fsp4HI GCNGC 6 cut(s) 564, 978, 1249, 1252, 1317, 1476
FspBI CTAG 1 cut(s) 1721
GlaI GCGC 2 cut(s) 510, 1621
GluI GCNGC 6 cut(s) 564, 978, 1249, 1252, 1317, 1476
GsaI CCCAGC 5 cut(s) 170, 215, 245, 293, 368
GsuI CTGGAG 4 cut(s) 102, 177, 255, 1185
HaeIII GGCC 4 cut(s) 760, 831, 1568, 1684
HapII CCGG 3 cut(s) 664, 1411, 1529
HhaI GCGC 2 cut(s) 511, 1622
Hin1II CATG 4 cut(s) 469, 968, 1463, 1598
Hin6I GCGC 2 cut(s) 509, 1620
HinP1I GCGC 2 cut(s) 509, 1620
HincII GTYRAC 2 cut(s) 401, 1296
HindII GTYRAC 2 cut(s) 401, 1296
HindIII AAGCTT 2 cut(s) 1076, 1270
HinfI GANTC 9 cut(s) 41, 469, 523, 1022, 1120, 1285, 1376, 1644, 1668
HpaII CCGG 3 cut(s) 664, 1411, 1529
HphI GGTGA 5 cut(s) 1122, 1200, 1288, 1294, 1435
Hpy166II GTNNAC 4 cut(s) 401, 1189, 1235, 1296
Hpy8I GTNNAC 4 cut(s) 401, 1189, 1235, 1296
HpyAV CCTTC 8 cut(s) 120, 168, 195, 243, 273, 613, 1164, 1273
HpyCH4III ACNGT 2 cut(s) 1019, 1576
HpyCH4IV ACGT 1 cut(s) 1093
HpyCH4V TGCA 7 cut(s) 791, 980, 1235, 1319, 1397, 1631, 1674
HpyF10VI GCNNNNNNNGC 6 cut(s) 965, 974, 986, 1316, 1565, 1628
HpyF3I CTNAG 6 cut(s) 225, 474, 750, 816, 1344, 1679
HpySE526I ACGT 1 cut(s) 1093
Hsp92II CATG 4 cut(s) 469, 968, 1463, 1598
HspAI GCGC 2 cut(s) 509, 1620
KpnI GGTACC 1 cut(s) 1343
Ksp22I TGATCA 2 cut(s) 492, 1081
Kzo9I GATC 4 cut(s) 448, 492, 1081, 1087
LguI GCTCTTC 1 cut(s) 1013
LmnI GCTCC 1 cut(s) 852
Lsp1109I GCAGC 5 cut(s) 964, 1235, 1238, 1303, 1462
LweI GCATC 3 cut(s) 976, 989, 1498
MaeI CTAG 1 cut(s) 1721
MaeII ACGT 1 cut(s) 1093
MaeIII GTNAC 5 cut(s) 625, 1019, 1144, 1206, 1582
MalI GATC 4 cut(s) 450, 494, 1083, 1089
MbiI CCGCTC 3 cut(s) 566, 1359, 1609
MboI GATC 4 cut(s) 448, 492, 1081, 1087
MflI RGATCY 1 cut(s) 448
MhlI GDGCHC 5 cut(s) 338, 822, 961, 1130, 1237
MluCI AATT 5 cut(s) 13, 504, 651, 872, 1193
MlyI GAGTC 4 cut(s) 1016, 1385, 1638, 1662
MmeI TCCRAC 5 cut(s) 618, 930, 1084, 1181, 1477
MseI TTAA 3 cut(s) 348, 825, 1115
MspA1I CMGCKG 1 cut(s) 1248
MspCI CTTAAG 1 cut(s) 1114
MspI CCGG 3 cut(s) 664, 1411, 1529
MspR9I CCNGG 2 cut(s) 1290, 1412
Mva1269I GAATGC 3 cut(s) 1035, 1221, 1319
MvaI CCWGG 1 cut(s) 1290
MvnI CGCG 2 cut(s) 861, 1620
MwoI GCNNNNNNNGC 6 cut(s) 965, 974, 986, 1316, 1565, 1628
NciI CCSGG 1 cut(s) 1412
NdeII GATC 4 cut(s) 448, 492, 1081, 1087
NlaIII CATG 4 cut(s) 469, 968, 1463, 1598
NlaIV GGNNCC 2 cut(s) 450, 1341
NmuCI GTSAC 3 cut(s) 625, 1019, 1206
PagI TCATGA 1 cut(s) 465
PciSI GCTCTTC 1 cut(s) 1013
PctI GAATGC 3 cut(s) 1035, 1221, 1319
PfeI GAWTC 5 cut(s) 41, 469, 523, 1120, 1285
PflFI GACNNNGTC 1 cut(s) 782
PfoI TCCNGGA 1 cut(s) 1410
PkrI GCNGC 6 cut(s) 565, 979, 1250, 1253, 1318, 1477
PleI GAGTC 4 cut(s) 1016, 1384, 1638, 1662
PpsI GAGTC 4 cut(s) 1016, 1384, 1638, 1662
PsiI TTATAA 2 cut(s) 657, 684
Psp1406I AACGTT 1 cut(s) 1093
Psp6I CCWGG 1 cut(s) 1288
PspFI CCCAGC 5 cut(s) 166, 211, 241, 289, 364
PspGI CCWGG 1 cut(s) 1288
PspN4I GGNNCC 2 cut(s) 450, 1341
PspPI GGNCC 2 cut(s) 784, 829
PstNI CAGNNNCTG 1 cut(s) 1251
PsuI RGATCY 1 cut(s) 448
PsyI GACNNNGTC 1 cut(s) 782
PvuII CAGCTG 1 cut(s) 1248
RsaI GTAC 3 cut(s) 811, 1341, 1745
RsaNI GTAC 3 cut(s) 810, 1340, 1744
SalI GTCGAC 1 cut(s) 1294
SapI GCTCTTC 1 cut(s) 1013
SaqAI TTAA 3 cut(s) 348, 825, 1115
SatI GCNGC 6 cut(s) 564, 978, 1249, 1252, 1317, 1476
Sau3AI GATC 4 cut(s) 448, 492, 1081, 1087
Sau96I GGNCC 2 cut(s) 784, 829
ScaI AGTACT 1 cut(s) 811
SchI GAGTC 4 cut(s) 1016, 1385, 1638, 1662
ScrFI CCNGG 2 cut(s) 1290, 1412
SduI GDGCHC 5 cut(s) 338, 822, 961, 1130, 1237
SfaNI GCATC 3 cut(s) 976, 989, 1498
SfcI CTRYAG 1 cut(s) 1041
SinI GGWCC 1 cut(s) 784
SmlI CTYRAG 2 cut(s) 1114, 1646
SmoI CTYRAG 2 cut(s) 1114, 1646
Sse9I AATT 5 cut(s) 13, 504, 651, 872, 1193
SsiI CCGC 4 cut(s) 36, 564, 1357, 1607
SspMI CTAG 1 cut(s) 1721
StyD4I CCNGG 2 cut(s) 1288, 1410
TaaI ACNGT 2 cut(s) 1019, 1576
TaiI ACGT 1 cut(s) 1096
TaqI TCGA 2 cut(s) 483, 1295
TasI AATT 5 cut(s) 13, 504, 651, 872, 1193
TatI WGTACW 1 cut(s) 809
TauI GCSGC 1 cut(s) 566
TfiI GAWTC 5 cut(s) 41, 469, 523, 1120, 1285
Tru1I TTAA 3 cut(s) 348, 825, 1115
Tru9I TTAA 3 cut(s) 348, 825, 1115
TscAI CASTG 1 cut(s) 804
TseFI GTSAC 3 cut(s) 625, 1019, 1206
TseI GCWGC 5 cut(s) 977, 1248, 1251, 1316, 1475
Tsp45I GTSAC 3 cut(s) 625, 1019, 1206
TspRI CASTG 1 cut(s) 804
Tth111I GACNNNGTC 1 cut(s) 782
Vha464I CTTAAG 1 cut(s) 1114
VneI GTGCAC 1 cut(s) 1233
VpaK11BI GGWCC 1 cut(s) 784
XagI CCTNNNNNAGG 1 cut(s) 617
XapI RAATTY 1 cut(s) 872
XmiI GTMKAC 2 cut(s) 1188, 1295
XspI CTAG 1 cut(s) 1721
ZrmI AGTACT 1 cut(s) 811
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.