RchiOBHm_Chr1g0318071

RNA helicase SDE3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0000595
Physical Location & Seq
Reverse (-)
5585122 .. 5588009
2888 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ54836

Sequence Viewer

Length: 1803 bp
ATGGGGTCATCTAATTCTAAACCACATCCTGCCTCCGCAGAATCCCCACCATATTATTCTAAACCATCTCCATCTTCCCCGAAACCCCCACCATATTCTTCTAAACCACCTTCATCTCCAGCTTGCCCACAACCCCCACCATATTCTTCTAAACCACCTTCATCCCCAGCTTCTTCTAAACCACCTTCATCTCCAGTTTCCCCACAACCCCCACCATATTCTTCTAAGCCACCTTCATCCCCAGCATATTCTTCTAAACCACCTTCATCTCCAGTTTCCCCACAACCCCCAGCATATTCTTCTAAACCATCTTCATCTCCATTTTCCCCAAAGTGCCCTCCTGTCTTTAAGTCAGTTCTACGCCCAGCCTCCTCCTCCTCAAATAATGATGATATAAGTCAACACCAACAATATGAGAAGAGAACAGCTTTTGTGTGTCTTGAAAAGGATCCATTACCTGTATTCATGATTCCTGAGGATATCGAAGCCTTGATCAAGAAAGAAATTGCGCCCAAAGTTCTGAATCGCCCATTATCTCCCACAACATACAAGGACTACTTTGCCGCTCTCTTATATTCTGAAGATTTCTACTTGGAGAAATGGAGTGATTTCCTTTTGAAGGGTGTGACATTAGCGTTGGATGAAAGTTCAATTTATAAAAACCGGAAAAAGGAAGCACTTTATAAAAATCGGAAAAAGGAAGATAAGGAAGACAAAACCTTTGTAGCATTTGAGCTTGATCGTGTTCCTGAGAAACGGCCATTCCTCTTATCAAGGGACTTGGTCCTTGCACGACCAGTGAGTAATAGTACTACTGAGCCCTTTAAGGGCCTCATCTATAAAGTTGTTGGGAGCAATCGCGTTTTGGTTGAATTTGAGGATGACTTTTATTCTTATCATCATTCCAACAAAAAATATGATATCAGCTTCTCATTCAATAGAGTTTGTTTGAAAAGAGCCCACCATGCCGTAAAAGCTGCATCAGATGCCTTGTTTCAGAACTTCCTCTTCCCTGACTGTGACTCACGAGCAAGCATTCCTACTGCACCAGCTCTGCTTTCTACTACCGGTCATAAGCTTGATCAAGATCAACGTTCGGCAGTCGGACATATCTTAAGGATTCAGGGCTCACCACCTTATGTAGTAACTGGTCCGGACTGTGAAGCCAAATCTTTTGCATATGTAAGAGAACCTTCAAGAACTGGAGTGGTTGTTAGTGAAGCCGTATACCAATTATGTCGGAAGTCACCAGAGTATCGCATTCTTATCTGTGCACCTACTAACAGCTGCTGCGATATGCTGATGAGAAGCTTGGTGAAGGTGATTCCAGAGTCGACTATGTTTCGTGCCAATGCTGCATTCCGAGAGAGAGATGAGGTACCTGAGGACATCCTCCGCTCATCCCTTTACAAAGAGTCTTGTTTTTCTTGTCCTGCAATAGAGAAACTCCGGGAATACAAAGTGATTTTCTCAACTTTCGTGAGTAGCTTCCGACTACATGATAAAGGCATAGCTGCTGGACATTTTAGCCATATTTTTCTGGTGGATGCTTCATCTGCTATTGAGCCGGAAACATTGGTCGCCCTGACTAACTTTGCTGTCAAGGCCACTAGTGTTATAGTTACTGGTAATCATGGAAATAGTCCGCTCTGGGTTCGCGCTGACATTGCAAGGAAAAAGGGACTCAAGATTTCATACTTTGAACGACTCTGCAAGCTCAGGCCATATAGAAGCCCCAGTCCAATGTTCATCACACAACTAGACCTGAACCAAAAGTCAGAGTACCAAACAAGACCTTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

600

Amino Acids

66.95

Weight (kDa)

9.23

Isoelectric Point (pI)

67.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MOV-10_beta-barrel PF21634 234 - 310 7.5e-10 Helicase MOV-10, beta-barrel domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000595)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g37100 FvH4_4g37100 FvH4_4g37100
malus_domestica MD13G1238300.v1.1 MD16G1243100.v1.1 MD16G1243200.v1.1
prunus_persica Prupe.1G070100_v2.0.a1 Prupe.1G070200_v2.0.a1 Prupe.1G070300_v2.0.a1 Prupe.1G070300_v2.0.a1
pyrus_communis pycom13g21070 pycom16g20390 pycom16g20400
rosa_chinensis RchiOBHm_Chr1g0318071 RchiOBHm_Chr1g0318111 RchiOBHm_Chr4g0446861 RchiOBHm_Chr4g0446881 RchiOBHm_Chr4g0446891
rosa_laevigata RLG00000005623 RLG00000005624 RLG00000009337 RLG00000030560 RLG00000030566
rosa_multiflora Rmu_co8177450.1_g000001 Rmu_sc0001753.1_g000012 Rmu_sc0001753.1_g000013 Rmu_sc0001753.1_g000014 Rmu_sc0001753.1_g000015 Rmu_sc0011424.1_g000009 Rmu_sc0011424.1_g000020 Rmu_sc0034555.1_g000001 Rmu_ssc0000008.1_g000002
rosa_roxburghii Rroxscaffold_4G00329530 Rroxscaffold_4G00329590 Rroxscaffold_4G00329800 Rroxscaffold_4G00329810 Rroxscaffold_4G00329860 Rroxscaffold_4G00329910 Rroxscaffold_4G00330030 Rroxscaffold_5G00343610 Rroxscaffold_5G00348280 Rroxscaffold_5G00387220 Rroxscaffold_5G00387230
rosa_rugosa Rorug01G0019100 Rorug01G0019100 Rorug01G0019500 Rorug01G0019600 Rorug04G0010100 Rorug04G0375200 Rorug04G0375300 Rorug04G0375400 Rorug04G0375500 Rorug04G0375600 Rorug04G0375700
rosa_samantha Rh1AG030700 Rh1AG030900 Rh1BG026900 Rh1CG029500 Rh1CG029700 Rh1DG040500 Rh4AG088100 Rh4AG436700 Rh4AG436800 Rh4AG436900 Rh4BG084700 Rh4BG445800 Rh4BG445900 Rh4BG446100 Rh4CG096300 Rh4CG463100 Rh4CG463200 Rh4CG463400 Rh4DG444500 Rh4DG444600 Rh4DG444700
rosa_wichuraiana Rw1G002420 Rw1G002440 Rw1G002480 Rw4G037360 Rw4G037370 Rw4G037380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 657, 684
Acc65I GGTACC 1 cut(s) 1378
AccB1I GGYRCC 1 cut(s) 1378
AccBSI CCGCTC 3 cut(s) 566, 1398, 1648
AccI GTMKAC 2 cut(s) 1227, 1334
AccII CGCG 2 cut(s) 861, 1659
AccIII TCCGGA 1 cut(s) 1153
AciI CCGC 4 cut(s) 36, 564, 1396, 1646
AclI AACGTT 1 cut(s) 1093
AclWI GGATC 2 cut(s) 443, 456
AcoI YGGCCR 1 cut(s) 758
AcsI RAATTY 1 cut(s) 872
AcuI CTGAAG 1 cut(s) 600
AfaI GTAC 3 cut(s) 811, 1380, 1784
AfiI CCNNNNNNNGG 3 cut(s) 619, 670, 827
AflII CTTAAG 1 cut(s) 1114
AgeI ACCGGT 1 cut(s) 1067
AgsI TTSAA 8 cut(s) 443, 619, 651, 872, 937, 952, 1197, 1703
AhlI ACTAGT 1 cut(s) 1610
AjuI GAANNNNNNNTTGG 2 cut(s) 1343, 1375
Alw21I GWGCWC 1 cut(s) 1276
Alw44I GTGCAC 1 cut(s) 1272
AlwI GGATC 2 cut(s) 443, 456
AlwNI CAGNNNCTG 1 cut(s) 1290
Aor13HI TCCGGA 1 cut(s) 1153
AoxI GGCC 4 cut(s) 758, 829, 1605, 1721
ApaLI GTGCAC 1 cut(s) 1272
ApeKI GCWGC 5 cut(s) 977, 1287, 1290, 1355, 1514
ApoI RAATTY 1 cut(s) 872
ArsI GACNNNNNNTTYG 2 cut(s) 704, 736
AsiGI ACCGGT 1 cut(s) 1067
Asp718I GGTACC 1 cut(s) 1378
AspLEI GCGC 2 cut(s) 511, 1661
AspS9I GGNCC 3 cut(s) 784, 829, 1151
AsuC2I CCSGG 1 cut(s) 1451
AsuHPI GGTGA 4 cut(s) 1122, 1239, 1327, 1333
AvaII GGWCC 2 cut(s) 784, 1151
AxyI CCTNAGG 2 cut(s) 474, 1383
BaeGI GKGCMC 2 cut(s) 338, 1276
BaeI ACNNNNGTAYC 2 cut(s) 1238, 1271
BamHI GGATCC 1 cut(s) 448
BanI GGYRCC 1 cut(s) 1378
BanII GRGCYC 3 cut(s) 822, 961, 1130
BauI CACGAG 1 cut(s) 1026
BbsI GAAGAC 1 cut(s) 717
Bbv12I GWGCWC 1 cut(s) 1276
BbvI GCAGC 5 cut(s) 964, 1274, 1277, 1342, 1501
BccI CCATC 3 cut(s) 73, 79, 316
BceAI ACGGC 3 cut(s) 773, 953, 1208
BclI TGATCA 2 cut(s) 492, 1081
BcnI CCSGG 1 cut(s) 1451
BcuI ACTAGT 1 cut(s) 1610
BfaI CTAG 2 cut(s) 1611, 1760
BfrI CTTAAG 1 cut(s) 1114
BisI GCNGC 6 cut(s) 564, 978, 1288, 1291, 1356, 1515
BlsI GCNGC 6 cut(s) 565, 979, 1289, 1292, 1357, 1516
BmcAI AGTACT 1 cut(s) 811
Bme1390I CCNGG 1 cut(s) 1451
Bme18I GGWCC 2 cut(s) 784, 1151
BmgT120I GGNCC 3 cut(s) 784, 829, 1151
BmiI GGNNCC 2 cut(s) 450, 1380
BmrFI CCNGG 1 cut(s) 1451
BmrI ACTGGG 1 cut(s) 1731
BmsI GCATC 3 cut(s) 976, 989, 1537
BmuI ACTGGG 1 cut(s) 1731
BpiI GAAGAC 1 cut(s) 717
BpmI CTGGAG 4 cut(s) 102, 177, 255, 1224
Bpu10I CCTNAGC 1 cut(s) 1718
BpuEI CTTGAG 1 cut(s) 1670
BpuMI CCSGG 1 cut(s) 1451
BsaBI GATNNNNATC 1 cut(s) 1086
BsaWI WCCGGW 3 cut(s) 663, 1067, 1153
BsaXI ACNNNNNCTCC 2 cut(s) 1197, 1227
Bsc4I CCNNNNNNNGG 3 cut(s) 619, 670, 827
Bse118I RCCGGY 1 cut(s) 1067
Bse1I ACTGG 7 cut(s) 194, 272, 797, 1153, 1207, 1630, 1737
Bse21I CCTNAGG 2 cut(s) 474, 1383
Bse3DI GCAATG 1 cut(s) 1665
Bse8I GATNNNNATC 1 cut(s) 1086
BseAI TCCGGA 1 cut(s) 1153
BseGI GGATG 8 cut(s) 25, 161, 236, 646, 886, 1389, 1400, 1552
BseJI GATNNNNATC 1 cut(s) 1086
BseLI CCNNNNNNNGG 3 cut(s) 619, 670, 827
BseMI GCAATG 1 cut(s) 1665
BseMII CTCAG 5 cut(s) 465, 741, 807, 1374, 1732
BseNI ACTGG 7 cut(s) 194, 272, 797, 1153, 1207, 1630, 1737
BseRI GAGGAG 3 cut(s) 361, 364, 367
BseSI GKGCMC 2 cut(s) 338, 1276
BseXI GCAGC 5 cut(s) 964, 1274, 1277, 1342, 1501
BseYI CCCAGC 4 cut(s) 166, 241, 289, 364
BsgI GTGCAG 1 cut(s) 1029
Bsh1236I CGCG 2 cut(s) 861, 1659
BshFI GGCC 4 cut(s) 760, 831, 1607, 1723
BshNI GGYRCC 1 cut(s) 1378
BshTI ACCGGT 1 cut(s) 1067
BsiHKAI GWGCWC 1 cut(s) 1276
BsiSI CCGG 5 cut(s) 664, 1068, 1154, 1450, 1568
BslFI GGGAC 2 cut(s) 791, 1695
BslI CCNNNNNNNGG 3 cut(s) 619, 670, 827
BsmFI GGGAC 2 cut(s) 791, 1695
BsmI GAATGC 3 cut(s) 1035, 1260, 1358
BsnI GGCC 4 cut(s) 760, 831, 1607, 1723
Bsp1286I GDGCHC 5 cut(s) 338, 822, 961, 1130, 1276
Bsp13I TCCGGA 1 cut(s) 1153
Bsp143I GATC 5 cut(s) 448, 492, 739, 1081, 1087
BspACI CCGC 4 cut(s) 36, 564, 1396, 1646
BspANI GGCC 4 cut(s) 760, 831, 1607, 1723
BspCNI CTCAG 5 cut(s) 466, 742, 808, 1375, 1731
BspEI TCCGGA 1 cut(s) 1153
BspFNI CGCG 2 cut(s) 861, 1659
BspHI TCATGA 1 cut(s) 465
BspLI GGNNCC 2 cut(s) 450, 1380
BspPI GGATC 2 cut(s) 443, 456
BspT107I GGYRCC 1 cut(s) 1378
BspTI CTTAAG 1 cut(s) 1114
BsrBI CCGCTC 3 cut(s) 566, 1398, 1648
BsrDI GCAATG 1 cut(s) 1665
BsrFI RCCGGY 1 cut(s) 1067
BsrI ACTGG 7 cut(s) 194, 272, 797, 1153, 1207, 1630, 1737
BssAI RCCGGY 1 cut(s) 1067
BssMI GATC 5 cut(s) 448, 492, 739, 1081, 1087
BssNAI GTATAC 1 cut(s) 1228
BssSI CACGAG 1 cut(s) 1026
Bst1107I GTATAC 1 cut(s) 1228
Bst2BI CACGAG 1 cut(s) 1026
Bst4CI ACNGT 2 cut(s) 1019, 1160
Bst6I CTCTTC 2 cut(s) 413, 1013
BstAFI CTTAAG 1 cut(s) 1114
BstAPI GCANNNNNTGC 1 cut(s) 986
BstC8I GCNNGC 3 cut(s) 124, 1033, 1715
BstDEI CTNAG 6 cut(s) 225, 474, 750, 816, 1383, 1718
BstENI CCTNNNNNAGG 1 cut(s) 617
BstF5I GGATG 8 cut(s) 25, 161, 236, 646, 886, 1389, 1400, 1552
BstFNI CGCG 2 cut(s) 861, 1659
BstHHI GCGC 2 cut(s) 511, 1661
BstKTI GATC 5 cut(s) 451, 495, 742, 1084, 1090
BstMBI GATC 5 cut(s) 448, 492, 739, 1081, 1087
BstMWI GCNNNNNNNGC 7 cut(s) 965, 974, 986, 1355, 1556, 1604, 1667
BstSCI CCNGG 1 cut(s) 1449
BstSLI GKGCMC 2 cut(s) 338, 1276
BstUI CGCG 2 cut(s) 861, 1659
BstV1I GCAGC 5 cut(s) 964, 1274, 1277, 1342, 1501
BstV2I GAAGAC 1 cut(s) 717
BstX2I RGATCY 1 cut(s) 448
BstYI RGATCY 1 cut(s) 448
BstZ17I GTATAC 1 cut(s) 1228
Bsu36I CCTNAGG 2 cut(s) 474, 1383
BsuRI GGCC 4 cut(s) 760, 831, 1607, 1723
BtsCI GGATG 8 cut(s) 25, 161, 236, 646, 886, 1389, 1400, 1552
BtsIMutI CAGTG 1 cut(s) 804
Cac8I GCNNGC 3 cut(s) 124, 1033, 1715
CaiI CAGNNNCTG 1 cut(s) 1290
CciI TCATGA 1 cut(s) 465
CfoI GCGC 2 cut(s) 511, 1661
Cfr10I RCCGGY 1 cut(s) 1067
Cfr13I GGNCC 3 cut(s) 784, 829, 1151
Csp6I GTAC 3 cut(s) 810, 1379, 1783
CspAI ACCGGT 1 cut(s) 1067
CviAII CATG 4 cut(s) 466, 965, 1499, 1634
CviQI GTAC 3 cut(s) 810, 1379, 1783
DdeI CTNAG 6 cut(s) 225, 474, 750, 816, 1383, 1718
DpnI GATC 5 cut(s) 450, 494, 741, 1083, 1089
DpnII GATC 5 cut(s) 448, 492, 739, 1081, 1087
EaeI YGGCCR 1 cut(s) 758
Eam1104I CTCTTC 2 cut(s) 413, 1013
EarI CTCTTC 2 cut(s) 413, 1013
Eco24I GRGCYC 3 cut(s) 822, 961, 1130
Eco32I GATATC 2 cut(s) 481, 922
Eco47I GGWCC 2 cut(s) 784, 1151
Eco57I CTGAAG 1 cut(s) 600
Eco81I CCTNAGG 2 cut(s) 474, 1383
EcoNI CCTNNNNNAGG 1 cut(s) 617
EcoO109I RGGNCCY 1 cut(s) 829
EcoRV GATATC 2 cut(s) 481, 922
EcoT38I GRGCYC 3 cut(s) 822, 961, 1130
FaeI CATG 4 cut(s) 469, 968, 1502, 1637
FalI AAGNNNNNCTT 4 cut(s) 542, 574, 1177, 1209
FaqI GGGAC 2 cut(s) 791, 1695
FatI CATG 4 cut(s) 465, 964, 1498, 1633
FauNDI CATATG 1 cut(s) 1180
FbaI TGATCA 2 cut(s) 492, 1081
FblI GTMKAC 2 cut(s) 1227, 1334
Fnu4HI GCNGC 6 cut(s) 564, 978, 1288, 1291, 1356, 1515
FokI GGATG 8 cut(s) 12, 148, 223, 653, 893, 1376, 1387, 1559
FriOI GRGCYC 3 cut(s) 822, 961, 1130
Fsp4HI GCNGC 6 cut(s) 564, 978, 1288, 1291, 1356, 1515
FspBI CTAG 2 cut(s) 1611, 1760
GlaI GCGC 2 cut(s) 510, 1660
GluI GCNGC 6 cut(s) 564, 978, 1288, 1291, 1356, 1515
GsaI CCCAGC 4 cut(s) 170, 245, 293, 368
GsuI CTGGAG 4 cut(s) 102, 177, 255, 1224
HaeIII GGCC 4 cut(s) 760, 831, 1607, 1723
HapII CCGG 5 cut(s) 664, 1068, 1154, 1450, 1568
HhaI GCGC 2 cut(s) 511, 1661
Hin1II CATG 4 cut(s) 469, 968, 1502, 1637
Hin6I GCGC 2 cut(s) 509, 1659
HinP1I GCGC 2 cut(s) 509, 1659
HincII GTYRAC 2 cut(s) 401, 1335
HindII GTYRAC 2 cut(s) 401, 1335
HindIII AAGCTT 2 cut(s) 1076, 1309
HpaII CCGG 5 cut(s) 664, 1068, 1154, 1450, 1568
HphI GGTGA 4 cut(s) 1122, 1239, 1327, 1333
Hpy166II GTNNAC 4 cut(s) 401, 1228, 1274, 1335
Hpy8I GTNNAC 4 cut(s) 401, 1228, 1274, 1335
HpyAV CCTTC 8 cut(s) 120, 168, 195, 243, 273, 613, 1203, 1312
HpyCH4III ACNGT 2 cut(s) 1019, 1160
HpyCH4IV ACGT 1 cut(s) 1093
HpyCH4V TGCA 9 cut(s) 791, 980, 1046, 1178, 1274, 1358, 1436, 1670, 1713
HpyF10VI GCNNNNNNNGC 7 cut(s) 965, 974, 986, 1355, 1556, 1604, 1667
HpyF3I CTNAG 6 cut(s) 225, 474, 750, 816, 1383, 1718
HpySE526I ACGT 1 cut(s) 1093
Hsp92II CATG 4 cut(s) 469, 968, 1502, 1637
HspAI GCGC 2 cut(s) 509, 1659
Kpn2I TCCGGA 1 cut(s) 1153
KpnI GGTACC 1 cut(s) 1382
Ksp22I TGATCA 2 cut(s) 492, 1081
Kzo9I GATC 5 cut(s) 448, 492, 739, 1081, 1087
LmnI GCTCC 1 cut(s) 852
Lsp1109I GCAGC 5 cut(s) 964, 1274, 1277, 1342, 1501
LweI GCATC 3 cut(s) 976, 989, 1537
MaeI CTAG 2 cut(s) 1611, 1760
MaeII ACGT 1 cut(s) 1093
MaeIII GTNAC 5 cut(s) 625, 1019, 1144, 1245, 1621
MalI GATC 5 cut(s) 450, 494, 741, 1083, 1089
MbiI CCGCTC 3 cut(s) 566, 1398, 1648
MboI GATC 5 cut(s) 448, 492, 739, 1081, 1087
MflI RGATCY 1 cut(s) 448
MhlI GDGCHC 5 cut(s) 338, 822, 961, 1130, 1276
MluCI AATT 5 cut(s) 13, 504, 651, 872, 1232
MlyI GAGTC 5 cut(s) 1016, 1340, 1424, 1677, 1701
MmeI TCCRAC 5 cut(s) 618, 930, 1084, 1220, 1516
MroI TCCGGA 1 cut(s) 1153
MseI TTAA 3 cut(s) 348, 825, 1115
MspA1I CMGCKG 1 cut(s) 1287
MspCI CTTAAG 1 cut(s) 1114
MspI CCGG 5 cut(s) 664, 1068, 1154, 1450, 1568
MspR9I CCNGG 1 cut(s) 1451
Mva1269I GAATGC 3 cut(s) 1035, 1260, 1358
MvnI CGCG 2 cut(s) 861, 1659
MwoI GCNNNNNNNGC 7 cut(s) 965, 974, 986, 1355, 1556, 1604, 1667
NciI CCSGG 1 cut(s) 1451
NdeI CATATG 1 cut(s) 1180
NdeII GATC 5 cut(s) 448, 492, 739, 1081, 1087
NlaIII CATG 4 cut(s) 469, 968, 1502, 1637
NlaIV GGNNCC 2 cut(s) 450, 1380
NmuCI GTSAC 3 cut(s) 625, 1019, 1245
PagI TCATGA 1 cut(s) 465
PctI GAATGC 3 cut(s) 1035, 1260, 1358
PfeI GAWTC 5 cut(s) 41, 469, 523, 1120, 1324
PflFI GACNNNGTC 1 cut(s) 782
PfoI TCCNGGA 1 cut(s) 1449
PinAI ACCGGT 1 cut(s) 1067
PkrI GCNGC 6 cut(s) 565, 979, 1289, 1292, 1357, 1516
PleI GAGTC 5 cut(s) 1016, 1339, 1423, 1677, 1701
PpsI GAGTC 5 cut(s) 1016, 1339, 1423, 1677, 1701
PsiI TTATAA 2 cut(s) 657, 684
Psp1406I AACGTT 1 cut(s) 1093
PspFI CCCAGC 4 cut(s) 166, 241, 289, 364
PspN4I GGNNCC 2 cut(s) 450, 1380
PspPI GGNCC 3 cut(s) 784, 829, 1151
PstNI CAGNNNCTG 1 cut(s) 1290
PsuI RGATCY 1 cut(s) 448
PsyI GACNNNGTC 1 cut(s) 782
PvuII CAGCTG 1 cut(s) 1287
RsaI GTAC 3 cut(s) 811, 1380, 1784
RsaNI GTAC 3 cut(s) 810, 1379, 1783
SalI GTCGAC 1 cut(s) 1333
SaqAI TTAA 3 cut(s) 348, 825, 1115
SatI GCNGC 6 cut(s) 564, 978, 1288, 1291, 1356, 1515
Sau3AI GATC 5 cut(s) 448, 492, 739, 1081, 1087
Sau96I GGNCC 3 cut(s) 784, 829, 1151
ScaI AGTACT 1 cut(s) 811
SchI GAGTC 5 cut(s) 1016, 1340, 1424, 1677, 1701
ScrFI CCNGG 1 cut(s) 1451
SduI GDGCHC 5 cut(s) 338, 822, 961, 1130, 1276
SfaNI GCATC 3 cut(s) 976, 989, 1537
SinI GGWCC 2 cut(s) 784, 1151
SmlI CTYRAG 2 cut(s) 1114, 1685
SmoI CTYRAG 2 cut(s) 1114, 1685
SpeI ACTAGT 1 cut(s) 1610
Sse9I AATT 5 cut(s) 13, 504, 651, 872, 1232
SsiI CCGC 4 cut(s) 36, 564, 1396, 1646
SspMI CTAG 2 cut(s) 1611, 1760
StyD4I CCNGG 1 cut(s) 1449
TaaI ACNGT 2 cut(s) 1019, 1160
TaiI ACGT 1 cut(s) 1096
TaqI TCGA 2 cut(s) 483, 1334
TasI AATT 5 cut(s) 13, 504, 651, 872, 1232
TatI WGTACW 1 cut(s) 809
TauI GCSGC 1 cut(s) 566
TfiI GAWTC 5 cut(s) 41, 469, 523, 1120, 1324
Tru1I TTAA 3 cut(s) 348, 825, 1115
Tru9I TTAA 3 cut(s) 348, 825, 1115
TscAI CASTG 1 cut(s) 804
TseFI GTSAC 3 cut(s) 625, 1019, 1245
TseI GCWGC 5 cut(s) 977, 1287, 1290, 1355, 1514
Tsp45I GTSAC 3 cut(s) 625, 1019, 1245
TspRI CASTG 1 cut(s) 804
Tth111I GACNNNGTC 1 cut(s) 782
Vha464I CTTAAG 1 cut(s) 1114
VneI GTGCAC 1 cut(s) 1272
VpaK11BI GGWCC 2 cut(s) 784, 1151
XagI CCTNNNNNAGG 1 cut(s) 617
XapI RAATTY 1 cut(s) 872
XmiI GTMKAC 2 cut(s) 1227, 1334
XspI CTAG 2 cut(s) 1611, 1760
ZrmI AGTACT 1 cut(s) 811
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.