pycom16g20400

RNA helicase SDE3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0000595
Physical Location & Seq
Forward (+)
18154971 .. 18155987
1017 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g20400.1

Sequence Viewer

Length: 1017 bp
ATGATCTTACCTGTTATACAGGGCTTTATATATCGTGTCATCAAGAGCAGTGTTGTTTTAGTAGAATTTGAAGACGACTTTTATGTCCAACATTATTCGACCCGAAAATATGATGTCAGCTTCTCATTCAATAGAGTCTGTTTGAAAAGAGCTCATCAAGCTGTACAAAATGCATCGGATGCTTTGTTTAGGAACTTCATCTTTCCTGAGTCTGTTTCGCGTACGAACATTCCCACTGCACCAGCGCTGATGTGCCCTGGTCATAAACTTGATGCCAATCAATTATCTGCAGTTCGTCATATTTTAAGCATTCGTGGCTCACCACCTTACCTATTGGTTGGCCAGCTGTGTGTTAAAAAGAGCGGTTCACCACCTTACCCATTGCTTGGCCAGCTCAGGGATGAAAAGACCGATTTTAGGCTATCGAGAACTGGGGTGGTGGTTAGTGAAGCAGTGCACCAGTTATGTCAAACTTCACACGGAAATAGGATCCTTGTATGTGCTCCTAGCAACCTCTGTTGTGATGAGCTTGTGAGAAGCTTGTTGAAATGGATTCCTAAGTCGGACATGTTTCAAGTGAATGCTGCATTCCGCGAGAAAGAGGAGGTACCTGATGACATCCTCCCGTCATGCCTTTACAAAGAGACTTACTTCTCTTGTCCTCCGATGGAGGAACTTTGCGAATTCAGAGTGATCTTCTCCACTTTCATGAGTAGCTTTCGGCTGCATGATAAAGGCCTAACTGCTGGACATTTTAGTCATATTTTTCTGGTGGATGCTTCGTCTTCTATTGAGCCGGAAACATTGGTGGCCTTGACTAGTTTCGCTGACAAAAATACTAGTGTTATAGTTACCGGTGAAGCAGAAAACCGATCGCATTGGGTTCGAGCTGACATGGCTAGGAAAAAGGGACTGAAGATTTCCCTGTTTGAAAGACTTTCCAAGTCAGTGCCATATTGTGTAGAAGCCTCAGTTCAATGTTCATCACACAATTGGAGCATTATCAAGAGTAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

339

Amino Acids

38.12

Weight (kDa)

8.54

Isoelectric Point (pI)

42.53

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000595)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g37100 FvH4_4g37100 FvH4_4g37100
malus_domestica MD13G1238300.v1.1 MD16G1243100.v1.1 MD16G1243200.v1.1
prunus_persica Prupe.1G070100_v2.0.a1 Prupe.1G070200_v2.0.a1 Prupe.1G070300_v2.0.a1 Prupe.1G070300_v2.0.a1
pyrus_communis pycom13g21070 pycom16g20390 pycom16g20400
rosa_chinensis RchiOBHm_Chr1g0318071 RchiOBHm_Chr1g0318111 RchiOBHm_Chr4g0446861 RchiOBHm_Chr4g0446881 RchiOBHm_Chr4g0446891
rosa_laevigata RLG00000005623 RLG00000005624 RLG00000009337 RLG00000030560 RLG00000030566
rosa_multiflora Rmu_co8177450.1_g000001 Rmu_sc0001753.1_g000012 Rmu_sc0001753.1_g000013 Rmu_sc0001753.1_g000014 Rmu_sc0001753.1_g000015 Rmu_sc0011424.1_g000009 Rmu_sc0011424.1_g000020 Rmu_sc0034555.1_g000001 Rmu_ssc0000008.1_g000002
rosa_roxburghii Rroxscaffold_4G00329530 Rroxscaffold_4G00329590 Rroxscaffold_4G00329800 Rroxscaffold_4G00329810 Rroxscaffold_4G00329860 Rroxscaffold_4G00329910 Rroxscaffold_4G00330030 Rroxscaffold_5G00343610 Rroxscaffold_5G00348280 Rroxscaffold_5G00387220 Rroxscaffold_5G00387230
rosa_rugosa Rorug01G0019100 Rorug01G0019100 Rorug01G0019500 Rorug01G0019600 Rorug04G0010100 Rorug04G0375200 Rorug04G0375300 Rorug04G0375400 Rorug04G0375500 Rorug04G0375600 Rorug04G0375700
rosa_samantha Rh1AG030700 Rh1AG030900 Rh1BG026900 Rh1CG029500 Rh1CG029700 Rh1DG040500 Rh4AG088100 Rh4AG436700 Rh4AG436800 Rh4AG436900 Rh4BG084700 Rh4BG445800 Rh4BG445900 Rh4BG446100 Rh4CG096300 Rh4CG463100 Rh4CG463200 Rh4CG463400 Rh4DG444500 Rh4DG444600 Rh4DG444700
rosa_wichuraiana Rw1G002420 Rw1G002440 Rw1G002480 Rw4G037360 Rw4G037370 Rw4G037380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 83, 756
Acc65I GGTACC 1 cut(s) 607
AccB1I GGYRCC 1 cut(s) 607
AccB7I CCANNNNNTGG 1 cut(s) 386
AccBSI CCGCTC 1 cut(s) 363
AccII CGCG 2 cut(s) 220, 594
AciI CCGC 2 cut(s) 363, 592
AclWI GGATC 2 cut(s) 484, 497
AcoI YGGCCR 2 cut(s) 340, 388
AcsI RAATTY 2 cut(s) 65, 683
AcuI CTGAAG 1 cut(s) 935
AfaI GTAC 3 cut(s) 165, 223, 609
AfeI AGCGCT 1 cut(s) 246
AfiI CCNNNNNNNGG 3 cut(s) 386, 397, 417
AflIII ACRYGT 1 cut(s) 567
AgeI ACCGGT 1 cut(s) 854
AgsI TTSAA 7 cut(s) 71, 130, 145, 547, 575, 932, 977
AhlI ACTAGT 2 cut(s) 818, 839
AjnI CCWGG 1 cut(s) 256
AluBI AGCT 9 cut(s) 120, 152, 161, 346, 394, 529, 540, 717, 890
AluI AGCT 9 cut(s) 120, 152, 161, 346, 394, 529, 540, 717, 890
Alw21I GWGCWC 3 cut(s) 154, 459, 505
Alw26I GTCTC 1 cut(s) 638
Alw44I GTGCAC 1 cut(s) 455
AlwI GGATC 2 cut(s) 484, 497
Aor51HI AGCGCT 1 cut(s) 246
AoxI GGCC 4 cut(s) 340, 388, 736, 810
ApaLI GTGCAC 1 cut(s) 455
ApeKI GCWGC 2 cut(s) 584, 724
ApoI RAATTY 2 cut(s) 65, 683
AsiGI ACCGGT 1 cut(s) 854
Asp718I GGTACC 1 cut(s) 607
AspLEI GCGC 1 cut(s) 247
AsuHPI GGTGA 3 cut(s) 312, 360, 869
BaeGI GKGCMC 2 cut(s) 257, 459
BalI TGGCCA 2 cut(s) 342, 390
BamHI GGATCC 1 cut(s) 489
BanI GGYRCC 1 cut(s) 607
BanII GRGCYC 1 cut(s) 154
BbsI GAAGAC 2 cut(s) 78, 777
Bbv12I GWGCWC 3 cut(s) 154, 459, 505
BbvI GCAGC 2 cut(s) 571, 711
BccI CCATC 1 cut(s) 661
BcgI CGANNNNNNTGC 2 cut(s) 866, 900
BciT130I CCWGG 1 cut(s) 258
BcoDI GTCTC 1 cut(s) 638
BcuI ACTAGT 2 cut(s) 818, 839
BfaI CTAG 4 cut(s) 507, 819, 840, 900
BfmI CTRYAG 1 cut(s) 288
BfoI RGCGCY 1 cut(s) 248
BisI GCNGC 2 cut(s) 585, 725
BlsI GCNGC 2 cut(s) 586, 726
Bme1390I CCNGG 1 cut(s) 258
BmiI GGNNCC 2 cut(s) 491, 609
BmrFI CCNGG 1 cut(s) 258
BmrI ACTGGG 1 cut(s) 441
BmsI GCATC 4 cut(s) 169, 182, 262, 766
BmuI ACTGGG 1 cut(s) 441
BpiI GAAGAC 2 cut(s) 78, 777
Bpu10I CCTNAGC 1 cut(s) 395
BsaBI GATNNNNATC 1 cut(s) 276
BsaJI CCNNGG 1 cut(s) 256
BsaWI WCCGGW 1 cut(s) 854
Bsc4I CCNNNNNNNGG 3 cut(s) 386, 397, 417
Bse118I RCCGGY 1 cut(s) 854
Bse1I ACTGG 2 cut(s) 436, 460
Bse3DI GCAATG 1 cut(s) 380
Bse8I GATNNNNATC 1 cut(s) 276
BseBI CCWGG 1 cut(s) 258
BseDI CCNNGG 1 cut(s) 256
BseGI GGATG 4 cut(s) 184, 406, 618, 781
BseJI GATNNNNATC 1 cut(s) 276
BseLI CCNNNNNNNGG 3 cut(s) 386, 397, 417
BseMI GCAATG 1 cut(s) 380
BseMII CTCAG 3 cut(s) 198, 409, 984
BseNI ACTGG 2 cut(s) 436, 460
BseRI GAGGAG 1 cut(s) 617
BseSI GKGCMC 2 cut(s) 257, 459
BseXI GCAGC 2 cut(s) 571, 711
BsgI GTGCAG 1 cut(s) 222
Bsh1236I CGCG 2 cut(s) 220, 594
Bsh1285I CGRYCG 1 cut(s) 875
BshFI GGCC 4 cut(s) 342, 390, 738, 812
BshNI GGYRCC 1 cut(s) 607
BshTI ACCGGT 1 cut(s) 854
BsiEI CGRYCG 1 cut(s) 875
BsiHKAI GWGCWC 3 cut(s) 154, 459, 505
BsiSI CCGG 2 cut(s) 797, 855
BsiWI CGTACG 1 cut(s) 221
BslFI GGGAC 1 cut(s) 924
BslI CCNNNNNNNGG 3 cut(s) 386, 397, 417
BsmAI GTCTC 1 cut(s) 638
BsmFI GGGAC 1 cut(s) 924
BsmI GAATGC 3 cut(s) 309, 586, 587
BsnI GGCC 4 cut(s) 342, 390, 738, 812
Bsp1286I GDGCHC 4 cut(s) 154, 257, 459, 505
Bsp1407I TGTACA 1 cut(s) 163
Bsp143I GATC 4 cut(s) 3, 489, 693, 872
BspACI CCGC 2 cut(s) 363, 592
BspANI GGCC 4 cut(s) 342, 390, 738, 812
BspCNI CTCAG 3 cut(s) 199, 408, 983
BspFNI CGCG 2 cut(s) 220, 594
BspHI TCATGA 1 cut(s) 708
BspLI GGNNCC 2 cut(s) 491, 609
BspMAI CTGCAG 1 cut(s) 292
BspPI GGATC 2 cut(s) 484, 497
BspT107I GGYRCC 1 cut(s) 607
BsrBI CCGCTC 1 cut(s) 363
BsrDI GCAATG 1 cut(s) 380
BsrFI RCCGGY 1 cut(s) 854
BsrGI TGTACA 1 cut(s) 163
BsrI ACTGG 2 cut(s) 436, 460
BssAI RCCGGY 1 cut(s) 854
BssECI CCNNGG 1 cut(s) 256
BssMI GATC 4 cut(s) 3, 489, 693, 872
Bst2UI CCWGG 1 cut(s) 258
BstAPI GCANNNNNTGC 1 cut(s) 179
BstAUI TGTACA 1 cut(s) 163
BstC8I GCNNGC 2 cut(s) 344, 392
BstDEI CTNAG 4 cut(s) 207, 395, 558, 970
BstF5I GGATG 4 cut(s) 184, 406, 618, 781
BstFNI CGCG 2 cut(s) 220, 594
BstH2I RGCGCY 1 cut(s) 248
BstHHI GCGC 1 cut(s) 247
BstKTI GATC 4 cut(s) 6, 492, 696, 875
BstMAI GTCTC 1 cut(s) 638
BstMBI GATC 4 cut(s) 3, 489, 693, 872
BstMCI CGRYCG 1 cut(s) 875
BstMWI GCNNNNNNNGC 5 cut(s) 158, 179, 315, 391, 896
BstNI CCWGG 1 cut(s) 258
BstNSI RCATGY 1 cut(s) 571
BstSCI CCNGG 1 cut(s) 256
BstSFI CTRYAG 1 cut(s) 288
BstSLI GKGCMC 2 cut(s) 257, 459
BstUI CGCG 2 cut(s) 220, 594
BstV1I GCAGC 2 cut(s) 571, 711
BstV2I GAAGAC 2 cut(s) 78, 777
BstX2I RGATCY 1 cut(s) 489
BstYI RGATCY 1 cut(s) 489
BsuRI GGCC 4 cut(s) 342, 390, 738, 812
BtsCI GGATG 4 cut(s) 184, 406, 618, 781
BtsI GCAGTG 3 cut(s) 55, 234, 459
BtsIMutI CAGTG 4 cut(s) 55, 234, 459, 954
Cac8I GCNNGC 2 cut(s) 344, 392
CciI TCATGA 1 cut(s) 708
CfoI GCGC 1 cut(s) 247
Cfr10I RCCGGY 1 cut(s) 854
Csp6I GTAC 3 cut(s) 164, 222, 608
CspAI ACCGGT 1 cut(s) 854
CviAII CATG 5 cut(s) 568, 630, 709, 728, 895
CviQI GTAC 3 cut(s) 164, 222, 608
DdeI CTNAG 4 cut(s) 207, 395, 558, 970
DpnI GATC 4 cut(s) 5, 491, 695, 874
DpnII GATC 4 cut(s) 3, 489, 693, 872
DrdI GACNNNNNNGTC 2 cut(s) 83, 756
DseDI GACNNNNNNGTC 2 cut(s) 83, 756
EaeI YGGCCR 2 cut(s) 340, 388
Ecl136II GAGCTC 1 cut(s) 152
Eco147I AGGCCT 1 cut(s) 738
Eco24I GRGCYC 1 cut(s) 154
Eco47III AGCGCT 1 cut(s) 246
Eco53kI GAGCTC 1 cut(s) 152
Eco57I CTGAAG 1 cut(s) 935
EcoICRI GAGCTC 1 cut(s) 152
EcoRI GAATTC 1 cut(s) 683
EcoRII CCWGG 1 cut(s) 256
EcoT22I ATGCAT 1 cut(s) 175
EcoT38I GRGCYC 1 cut(s) 154
FaeI CATG 5 cut(s) 571, 633, 712, 731, 898
FaqI GGGAC 1 cut(s) 924
FatI CATG 5 cut(s) 567, 629, 708, 727, 894
Fnu4HI GCNGC 2 cut(s) 585, 725
FokI GGATG 4 cut(s) 191, 413, 605, 788
FriOI GRGCYC 1 cut(s) 154
Fsp4HI GCNGC 2 cut(s) 585, 725
FspBI CTAG 4 cut(s) 507, 819, 840, 900
GlaI GCGC 1 cut(s) 246
GluI GCNGC 2 cut(s) 585, 725
HaeII RGCGCY 1 cut(s) 248
HaeIII GGCC 4 cut(s) 342, 390, 738, 812
HapII CCGG 2 cut(s) 797, 855
HhaI GCGC 1 cut(s) 247
Hin1II CATG 5 cut(s) 571, 633, 712, 731, 898
Hin6I GCGC 1 cut(s) 245
HinP1I GCGC 1 cut(s) 245
HindIII AAGCTT 1 cut(s) 538
HinfI GANTC 3 cut(s) 135, 209, 553
HpaII CCGG 2 cut(s) 797, 855
HphI GGTGA 3 cut(s) 312, 360, 869
Hpy166II GTNNAC 2 cut(s) 368, 457
Hpy188I TCNGA 4 cut(s) 178, 565, 666, 689
Hpy188III TCNNGA 5 cut(s) 43, 206, 426, 709, 1006
Hpy8I GTNNAC 2 cut(s) 368, 457
HpyCH4V TGCA 6 cut(s) 173, 239, 290, 457, 587, 727
HpyF10VI GCNNNNNNNGC 5 cut(s) 158, 179, 315, 391, 896
HpyF3I CTNAG 4 cut(s) 207, 395, 558, 970
Hsp92II CATG 5 cut(s) 571, 633, 712, 731, 898
HspAI GCGC 1 cut(s) 245
KpnI GGTACC 1 cut(s) 611
Kzo9I GATC 4 cut(s) 3, 489, 693, 872
LmnI GCTCC 2 cut(s) 508, 996
Lsp1109I GCAGC 2 cut(s) 571, 711
LweI GCATC 4 cut(s) 169, 182, 262, 766
MaeI CTAG 4 cut(s) 507, 819, 840, 900
MaeIII GTNAC 1 cut(s) 850
MalI GATC 4 cut(s) 5, 491, 695, 874
MbiI CCGCTC 1 cut(s) 363
MboI GATC 4 cut(s) 3, 489, 693, 872
MboII GAAGA 4 cut(s) 83, 688, 777, 928
MfeI CAATTG 1 cut(s) 991
MflI RGATCY 1 cut(s) 489
MhlI GDGCHC 4 cut(s) 154, 257, 459, 505
MlsI TGGCCA 2 cut(s) 342, 390
MluCI AATT 4 cut(s) 65, 281, 683, 991
MluNI TGGCCA 2 cut(s) 342, 390
MlyI GAGTC 2 cut(s) 144, 218
MmeI TCCRAC 2 cut(s) 112, 543
MnlI CCTC 7 cut(s) 524, 595, 598, 632, 664, 672, 979
Mox20I TGGCCA 2 cut(s) 342, 390
Mph1103I ATGCAT 1 cut(s) 175
MscI TGGCCA 2 cut(s) 342, 390
MseI TTAA 2 cut(s) 305, 354
MslI CAYNNNNRTG 1 cut(s) 707
Msp20I TGGCCA 2 cut(s) 342, 390
MspA1I CMGCKG 1 cut(s) 346
MspI CCGG 2 cut(s) 797, 855
MspR9I CCNGG 1 cut(s) 258
MunI CAATTG 1 cut(s) 991
Mva1269I GAATGC 3 cut(s) 309, 586, 587
MvaI CCWGG 1 cut(s) 258
MvnI CGCG 2 cut(s) 220, 594
MwoI GCNNNNNNNGC 5 cut(s) 158, 179, 315, 391, 896
NdeII GATC 4 cut(s) 3, 489, 693, 872
NlaIII CATG 5 cut(s) 571, 633, 712, 731, 898
NlaIV GGNNCC 2 cut(s) 491, 609
NsiI ATGCAT 1 cut(s) 175
NspI RCATGY 1 cut(s) 571
PagI TCATGA 1 cut(s) 708
PceI AGGCCT 1 cut(s) 738
PciI ACATGT 1 cut(s) 567
PctI GAATGC 3 cut(s) 309, 586, 587
PfeI GAWTC 1 cut(s) 553
Pfl23II CGTACG 1 cut(s) 221
PflMI CCANNNNNTGG 1 cut(s) 386
PinAI ACCGGT 1 cut(s) 854
PkrI GCNGC 2 cut(s) 586, 726
Ple19I CGATCG 1 cut(s) 875
PleI GAGTC 2 cut(s) 143, 217
PpsI GAGTC 2 cut(s) 143, 217
PscI ACATGT 1 cut(s) 567
Psp124BI GAGCTC 1 cut(s) 154
Psp6I CCWGG 1 cut(s) 256
PspGI CCWGG 1 cut(s) 256
PspLI CGTACG 1 cut(s) 221
PspN4I GGNNCC 2 cut(s) 491, 609
PstI CTGCAG 1 cut(s) 292
PsuI RGATCY 1 cut(s) 489
PvuI CGATCG 1 cut(s) 875
PvuII CAGCTG 1 cut(s) 346
RsaI GTAC 3 cut(s) 165, 223, 609
RsaNI GTAC 3 cut(s) 164, 222, 608
RseI CAYNNNNRTG 1 cut(s) 707
SacI GAGCTC 1 cut(s) 154
SaqAI TTAA 2 cut(s) 305, 354
SatI GCNGC 2 cut(s) 585, 725
Sau3AI GATC 4 cut(s) 3, 489, 693, 872
SchI GAGTC 2 cut(s) 144, 218
ScrFI CCNGG 1 cut(s) 258
SduI GDGCHC 4 cut(s) 154, 257, 459, 505
SfaNI GCATC 4 cut(s) 169, 182, 262, 766
SfcI CTRYAG 1 cut(s) 288
SmiMI CAYNNNNRTG 1 cut(s) 707
SpeI ACTAGT 2 cut(s) 818, 839
Sse9I AATT 4 cut(s) 65, 281, 683, 991
SseBI AGGCCT 1 cut(s) 738
SsiI CCGC 2 cut(s) 363, 592
SspMI CTAG 4 cut(s) 507, 819, 840, 900
SstI GAGCTC 1 cut(s) 154
StuI AGGCCT 1 cut(s) 738
StyD4I CCNGG 1 cut(s) 256
TaqI TCGA 3 cut(s) 98, 425, 886
TaqII GACCGA 1 cut(s) 425
TasI AATT 4 cut(s) 65, 281, 683, 991
TatI WGTACW 1 cut(s) 163
TfiI GAWTC 1 cut(s) 553
Tru1I TTAA 2 cut(s) 305, 354
Tru9I TTAA 2 cut(s) 305, 354
TscAI CASTG 4 cut(s) 55, 241, 459, 954
TseI GCWGC 2 cut(s) 584, 724
TspDTI ATGAA 4 cut(s) 187, 417, 697, 972
TspGWI ACGGA 1 cut(s) 495
TspRI CASTG 4 cut(s) 55, 241, 459, 954
Van91I CCANNNNNTGG 1 cut(s) 386
VneI GTGCAC 1 cut(s) 455
XapI RAATTY 2 cut(s) 65, 683
XceI RCATGY 1 cut(s) 571
XspI CTAG 4 cut(s) 507, 819, 840, 900
Zsp2I ATGCAT 1 cut(s) 175
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.