Domain in histone families 1 and 5
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 3 cut(s) | 351, 1101, 1755 |
| Acc36I | ACCTGC | 2 cut(s) | 1238, 1521 |
| AccB1I | GGYRCC | 2 cut(s) | 2362, 2655 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 74 |
| AccBSI | CCGCTC | 1 cut(s) | 381 |
| AccI | GTMKAC | 3 cut(s) | 1252, 2551, 3438 |
| AccII | CGCG | 2 cut(s) | 122, 2653 |
| AccIII | TCCGGA | 1 cut(s) | 850 |
| AciI | CCGC | 9 cut(s) | 381, 695, 2565, 2638, 2651, 2653, 2971, 3482, 3802 |
| AclWI | GGATC | 7 cut(s) | 853, 1507, 1714, 2484, 2830, 3108, 3302 |
| AcoI | YGGCCR | 1 cut(s) | 3522 |
| AcsI | RAATTY | 6 cut(s) | 299, 897, 1839, 1944, 3227, 3767 |
| AfaI | GTAC | 5 cut(s) | 21, 723, 2869, 3715, 3823 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 74, 1019, 1914, 2168 |
| AflIII | ACRYGT | 1 cut(s) | 1196 |
| AhlI | ACTAGT | 1 cut(s) | 3703 |
| AjiI | CACGTC | 2 cut(s) | 596, 2673 |
| AjnI | CCWGG | 1 cut(s) | 2626 |
| AleI | CACNNNNGTG | 2 cut(s) | 2290, 3351 |
| Alw21I | GWGCWC | 2 cut(s) | 2428, 2978 |
| Alw26I | GTCTC | 2 cut(s) | 752, 3799 |
| Alw44I | GTGCAC | 1 cut(s) | 2974 |
| AlwI | GGATC | 7 cut(s) | 853, 1507, 1714, 2484, 2830, 3108, 3302 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 254 |
| Aor13HI | TCCGGA | 1 cut(s) | 850 |
| AoxI | GGCC | 4 cut(s) | 2749, 3503, 3522, 3899 |
| ApaLI | GTGCAC | 1 cut(s) | 2974 |
| ApoI | RAATTY | 6 cut(s) | 299, 897, 1839, 1944, 3227, 3767 |
| Asp700I | GAANNNNTTC | 3 cut(s) | 1353, 1839, 3051 |
| AspA2I | CCTAGG | 2 cut(s) | 2540, 3061 |
| AspLEI | GCGC | 1 cut(s) | 3264 |
| AspS9I | GGNCC | 1 cut(s) | 3065 |
| AsuHPI | GGTGA | 6 cut(s) | 209, 838, 1280, 1285, 1492, 3327 |
| AvaII | GGWCC | 1 cut(s) | 3065 |
| AvrII | CCTAGG | 2 cut(s) | 2540, 3061 |
| BaeGI | GKGCMC | 1 cut(s) | 2978 |
| BalI | TGGCCA | 1 cut(s) | 3524 |
| BanI | GGYRCC | 2 cut(s) | 2362, 2655 |
| BauI | CACGAG | 1 cut(s) | 3179 |
| BbsI | GAAGAC | 2 cut(s) | 639, 1248 |
| Bbv12I | GWGCWC | 2 cut(s) | 2428, 2978 |
| BccI | CCATC | 7 cut(s) | 836, 1739, 2233, 2341, 2372, 2494, 3673 |
| BceAI | ACGGC | 2 cut(s) | 896, 3087 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 2402, 2436 |
| BciT130I | CCWGG | 1 cut(s) | 2628 |
| BciVI | GTATCC | 1 cut(s) | 3328 |
| BclI | TGATCA | 1 cut(s) | 2127 |
| BcoDI | GTCTC | 2 cut(s) | 752, 3799 |
| BcuI | ACTAGT | 1 cut(s) | 3703 |
| BfaI | CTAG | 9 cut(s) | 99, 806, 1061, 1256, 1955, 2541, 2826, 3062, 3704 |
| BfmI | CTRYAG | 3 cut(s) | 3240, 3435, 3630 |
| BfuAI | ACCTGC | 2 cut(s) | 1238, 1521 |
| BfuI | GTATCC | 1 cut(s) | 3328 |
| BglII | AGATCT | 1 cut(s) | 2891 |
| BlnI | CCTAGG | 2 cut(s) | 2540, 3061 |
| BlpI | GCTNAGC | 1 cut(s) | 480 |
| Bme1390I | CCNGG | 1 cut(s) | 2628 |
| Bme18I | GGWCC | 1 cut(s) | 3065 |
| BmgBI | CACGTC | 2 cut(s) | 596, 2673 |
| BmgT120I | GGNCC | 1 cut(s) | 3065 |
| BmiI | GGNNCC | 4 cut(s) | 456, 591, 2364, 2657 |
| BmrFI | CCNGG | 1 cut(s) | 2628 |
| BmrI | ACTGGG | 2 cut(s) | 2956, 3804 |
| BmuI | ACTGGG | 2 cut(s) | 2956, 3804 |
| BoxI | GACNNNNGTC | 1 cut(s) | 1248 |
| BpiI | GAAGAC | 2 cut(s) | 639, 1248 |
| BpmI | CTGGAG | 1 cut(s) | 1695 |
| Bpu10I | CCTNAGC | 1 cut(s) | 153 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 480 |
| BpuEI | CTTGAG | 4 cut(s) | 370, 2119, 2543, 3191 |
| BsaBI | GATNNNNATC | 1 cut(s) | 28 |
| BsaI | GGTCTC | 1 cut(s) | 3799 |
| BsaJI | CCNNGG | 8 cut(s) | 714, 1908, 2167, 2318, 2540, 2651, 3001, 3061 |
| BsaWI | WCCGGW | 1 cut(s) | 850 |
| BsaXI | ACNNNNNCTCC | 6 cut(s) | 375, 405, 2402, 2432, 3796, 3826 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 74, 1019, 1914, 2168 |
| Bse118I | RCCGGY | 2 cut(s) | 3536, 3743 |
| Bse3DI | GCAATG | 3 cut(s) | 1134, 1589, 1901 |
| Bse8I | GATNNNNATC | 1 cut(s) | 28 |
| BseAI | TCCGGA | 1 cut(s) | 850 |
| BseBI | CCWGG | 1 cut(s) | 2628 |
| BseDI | CCNNGG | 8 cut(s) | 714, 1908, 2167, 2318, 2540, 2651, 3001, 3061 |
| BseGI | GGATG | 8 cut(s) | 1061, 1513, 1861, 2093, 2197, 2383, 2863, 3849 |
| BseJI | GATNNNNATC | 1 cut(s) | 28 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 74, 1019, 1914, 2168 |
| BseMI | GCAATG | 3 cut(s) | 1134, 1589, 1901 |
| BseMII | CTCAG | 6 cut(s) | 26, 144, 246, 471, 945, 2178 |
| BseRI | GAGGAG | 6 cut(s) | 1035, 2210, 2399, 2456, 2876, 3383 |
| BseSI | GKGCMC | 1 cut(s) | 2978 |
| BseYI | CCCAGC | 3 cut(s) | 484, 2464, 3171 |
| BsgI | GTGCAG | 1 cut(s) | 1974 |
| Bsh1236I | CGCG | 2 cut(s) | 122, 2653 |
| Bsh1285I | CGRYCG | 1 cut(s) | 1018 |
| BshFI | GGCC | 4 cut(s) | 2751, 3505, 3524, 3901 |
| BshNI | GGYRCC | 2 cut(s) | 2362, 2655 |
| BsiEI | CGRYCG | 1 cut(s) | 1018 |
| BsiHKAI | GWGCWC | 2 cut(s) | 2428, 2978 |
| BsiSI | CCGG | 5 cut(s) | 422, 851, 3311, 3537, 3744 |
| BsiWI | CGTACG | 1 cut(s) | 19 |
| BslFI | GGGAC | 1 cut(s) | 1926 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 74, 1019, 1914, 2168 |
| BsmAI | GTCTC | 2 cut(s) | 752, 3799 |
| BsmFI | GGGAC | 1 cut(s) | 1926 |
| BsmI | GAATGC | 2 cut(s) | 1187, 2904 |
| BsnI | GGCC | 4 cut(s) | 2751, 3505, 3524, 3901 |
| Bso31I | GGTCTC | 1 cut(s) | 3799 |
| Bsp1286I | GDGCHC | 2 cut(s) | 2428, 2978 |
| Bsp13I | TCCGGA | 1 cut(s) | 850 |
| Bsp1407I | TGTACA | 1 cut(s) | 2867 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 480 |
| Bsp19I | CCATGG | 2 cut(s) | 714, 2167 |
| Bsp68I | TCGCGA | 1 cut(s) | 122 |
| BspACI | CCGC | 9 cut(s) | 381, 695, 2565, 2638, 2651, 2653, 2971, 3482, 3802 |
| BspANI | GGCC | 4 cut(s) | 2751, 3505, 3524, 3901 |
| BspCNI | CTCAG | 6 cut(s) | 25, 145, 247, 472, 946, 2179 |
| BspEI | TCCGGA | 1 cut(s) | 850 |
| BspFNI | CGCG | 2 cut(s) | 122, 2653 |
| BspHI | TCATGA | 2 cut(s) | 337, 3376 |
| BspLI | GGNNCC | 4 cut(s) | 456, 591, 2364, 2657 |
| BspMI | ACCTGC | 2 cut(s) | 1238, 1521 |
| BspPI | GGATC | 7 cut(s) | 853, 1507, 1714, 2484, 2830, 3108, 3302 |
| BspQI | GCTCTTC | 1 cut(s) | 2715 |
| BspT107I | GGYRCC | 2 cut(s) | 2362, 2655 |
| BspTNI | GGTCTC | 1 cut(s) | 3799 |
| BsrBI | CCGCTC | 1 cut(s) | 381 |
| BsrDI | GCAATG | 3 cut(s) | 1134, 1589, 1901 |
| BsrFI | RCCGGY | 2 cut(s) | 3536, 3743 |
| BsrGI | TGTACA | 1 cut(s) | 2867 |
| BssAI | RCCGGY | 2 cut(s) | 3536, 3743 |
| BssECI | CCNNGG | 8 cut(s) | 714, 1908, 2167, 2318, 2540, 2651, 3001, 3061 |
| BssNAI | GTATAC | 1 cut(s) | 2552 |
| BssSI | CACGAG | 1 cut(s) | 3179 |
| BssT1I | CCWWGG | 5 cut(s) | 714, 2167, 2540, 3001, 3061 |
| Bst1107I | GTATAC | 1 cut(s) | 2552 |
| Bst2BI | CACGAG | 1 cut(s) | 3179 |
| Bst2UI | CCWGG | 1 cut(s) | 2628 |
| Bst4CI | ACNGT | 8 cut(s) | 323, 410, 1875, 1917, 2885, 3287, 3436, 3835 |
| Bst6I | CTCTTC | 5 cut(s) | 49, 467, 600, 750, 2715 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 997, 3259 |
| BstAUI | TGTACA | 1 cut(s) | 2867 |
| BstC8I | GCNNGC | 3 cut(s) | 96, 120, 2394 |
| BstDEI | CTNAG | 9 cut(s) | 12, 153, 255, 371, 480, 954, 1032, 2187, 2367 |
| BstDSI | CCRYGG | 4 cut(s) | 714, 1908, 2167, 2651 |
| BstF5I | GGATG | 8 cut(s) | 1061, 1513, 1861, 2093, 2197, 2383, 2863, 3849 |
| BstFNI | CGCG | 2 cut(s) | 122, 2653 |
| BstHHI | GCGC | 1 cut(s) | 3264 |
| BstMAI | GTCTC | 2 cut(s) | 752, 3799 |
| BstMCI | CGRYCG | 1 cut(s) | 1018 |
| BstNI | CCWGG | 1 cut(s) | 2628 |
| BstNSI | RCATGY | 3 cut(s) | 575, 1200, 2514 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 1248 |
| BstSCI | CCNGG | 1 cut(s) | 2626 |
| BstSFI | CTRYAG | 3 cut(s) | 3240, 3435, 3630 |
| BstSLI | GKGCMC | 1 cut(s) | 2978 |
| BstUI | CGCG | 2 cut(s) | 122, 2653 |
| BstV2I | GAAGAC | 2 cut(s) | 639, 1248 |
| BstX2I | RGATCY | 5 cut(s) | 845, 1499, 1706, 2822, 2891 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 3817 |
| BstYI | RGATCY | 5 cut(s) | 845, 1499, 1706, 2822, 2891 |
| BstZ17I | GTATAC | 1 cut(s) | 2552 |
| BsuI | GTATCC | 1 cut(s) | 3328 |
| BsuRI | GGCC | 4 cut(s) | 2751, 3505, 3524, 3901 |
| BtgI | CCRYGG | 4 cut(s) | 714, 1908, 2167, 2651 |
| BtgZI | GCGATG | 3 cut(s) | 3555, 3660, 3762 |
| BtrI | CACGTC | 2 cut(s) | 596, 2673 |
| BtsCI | GGATG | 8 cut(s) | 1061, 1513, 1861, 2093, 2197, 2383, 2863, 3849 |
| BtsI | GCAGTG | 3 cut(s) | 1380, 1572, 1788 |
| BtsIMutI | CAGTG | 9 cut(s) | 1365, 1380, 1425, 1572, 1788, 1880, 2890, 3042, 3831 |
| BtuMI | TCGCGA | 1 cut(s) | 122 |
| BveI | ACCTGC | 2 cut(s) | 1238, 1521 |
| Cac8I | GCNNGC | 3 cut(s) | 96, 120, 2394 |
| CaiI | CAGNNNCTG | 1 cut(s) | 254 |
| CciI | TCATGA | 2 cut(s) | 337, 3376 |
| CfoI | GCGC | 1 cut(s) | 3264 |
| Cfr10I | RCCGGY | 2 cut(s) | 3536, 3743 |
| Cfr13I | GGNCC | 1 cut(s) | 3065 |
| Cfr42I | CCGCGG | 1 cut(s) | 2654 |
| CseI | GACGC | 2 cut(s) | 1253, 2636 |
| Csp6I | GTAC | 5 cut(s) | 20, 722, 2868, 3714, 3822 |
| CviQI | GTAC | 5 cut(s) | 20, 722, 2868, 3714, 3822 |
| DdeI | CTNAG | 9 cut(s) | 12, 153, 255, 371, 480, 954, 1032, 2187, 2367 |
| DrdI | GACNNNNNNGTC | 3 cut(s) | 351, 1101, 1755 |
| DseDI | GACNNNNNNGTC | 3 cut(s) | 351, 1101, 1755 |
| EaeI | YGGCCR | 1 cut(s) | 3522 |
| Eam1104I | CTCTTC | 5 cut(s) | 49, 467, 600, 750, 2715 |
| EarI | CTCTTC | 5 cut(s) | 49, 467, 600, 750, 2715 |
| Eco130I | CCWWGG | 5 cut(s) | 714, 2167, 2540, 3001, 3061 |
| Eco147I | AGGCCT | 2 cut(s) | 3505, 3901 |
| Eco31I | GGTCTC | 1 cut(s) | 3799 |
| Eco47I | GGWCC | 1 cut(s) | 3065 |
| EcoRII | CCWGG | 1 cut(s) | 2626 |
| EcoT14I | CCWWGG | 5 cut(s) | 714, 2167, 2540, 3001, 3061 |
| EcoT22I | ATGCAT | 2 cut(s) | 577, 2512 |
| ErhI | CCWWGG | 5 cut(s) | 714, 2167, 2540, 3001, 3061 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 54, 86 |
| FaqI | GGGAC | 1 cut(s) | 1926 |
| FauI | CCCGC | 1 cut(s) | 2558 |
| FauNDI | CATATG | 1 cut(s) | 3267 |
| FbaI | TGATCA | 1 cut(s) | 2127 |
| FblI | GTMKAC | 3 cut(s) | 1252, 2551, 3438 |
| FokI | GGATG | 8 cut(s) | 1068, 1520, 1868, 2080, 2204, 2390, 2870, 3856 |
| FspBI | CTAG | 9 cut(s) | 99, 806, 1061, 1256, 1955, 2541, 2826, 3062, 3704 |
| GlaI | GCGC | 1 cut(s) | 3263 |
| GsaI | CCCAGC | 3 cut(s) | 488, 2468, 3175 |
| GsuI | CTGGAG | 1 cut(s) | 1695 |
| HaeIII | GGCC | 4 cut(s) | 2751, 3505, 3524, 3901 |
| HapII | CCGG | 5 cut(s) | 422, 851, 3311, 3537, 3744 |
| HgaI | GACGC | 2 cut(s) | 1253, 2636 |
| HhaI | GCGC | 1 cut(s) | 3264 |
| Hin6I | GCGC | 1 cut(s) | 3262 |
| HinP1I | GCGC | 1 cut(s) | 3262 |
| HincII | GTYRAC | 2 cut(s) | 2930, 3875 |
| HindII | GTYRAC | 2 cut(s) | 2930, 3875 |
| HindIII | AAGCTT | 1 cut(s) | 92 |
| HpaII | CCGG | 5 cut(s) | 422, 851, 3311, 3537, 3744 |
| HphI | GGTGA | 6 cut(s) | 209, 838, 1280, 1285, 1492, 3327 |
| Hpy166II | GTNNAC | 8 cut(s) | 1253, 2552, 2670, 2868, 2930, 2976, 3439, 3875 |
| Hpy8I | GTNNAC | 8 cut(s) | 1253, 2552, 2670, 2868, 2930, 2976, 3439, 3875 |
| HpyAV | CCTTC | 9 cut(s) | 35, 44, 238, 263, 1922, 2708, 2722, 2853, 3891 |
| HpyCH4III | ACNGT | 8 cut(s) | 323, 410, 1875, 1917, 2885, 3287, 3436, 3835 |
| HpyCH4IV | ACGT | 5 cut(s) | 595, 636, 1654, 2672, 3565 |
| HpyF3I | CTNAG | 9 cut(s) | 12, 153, 255, 371, 480, 954, 1032, 2187, 2367 |
| HpySE526I | ACGT | 5 cut(s) | 595, 636, 1654, 2672, 3565 |
| HspAI | GCGC | 1 cut(s) | 3262 |
| Kpn2I | TCCGGA | 1 cut(s) | 850 |
| Ksp22I | TGATCA | 1 cut(s) | 2127 |
| KspI | CCGCGG | 1 cut(s) | 2654 |
| LguI | GCTCTTC | 1 cut(s) | 2715 |
| LmnI | GCTCC | 9 cut(s) | 454, 988, 1594, 2197, 2386, 2410, 2443, 3284, 3370 |
| MaeI | CTAG | 9 cut(s) | 99, 806, 1061, 1256, 1955, 2541, 2826, 3062, 3704 |
| MaeII | ACGT | 5 cut(s) | 595, 636, 1654, 2672, 3565 |
| MbiI | CCGCTC | 1 cut(s) | 381 |
| MfeI | CAATTG | 1 cut(s) | 2043 |
| MflI | RGATCY | 5 cut(s) | 845, 1499, 1706, 2822, 2891 |
| MhlI | GDGCHC | 2 cut(s) | 2428, 2978 |
| MlsI | TGGCCA | 1 cut(s) | 3524 |
| MluNI | TGGCCA | 1 cut(s) | 3524 |
| MlyI | GAGTC | 6 cut(s) | 172, 339, 361, 799, 811, 3434 |
| MmeI | TCCRAC | 1 cut(s) | 3764 |
| Mox20I | TGGCCA | 1 cut(s) | 3524 |
| Mph1103I | ATGCAT | 2 cut(s) | 577, 2512 |
| MroI | TCCGGA | 1 cut(s) | 850 |
| MroXI | GAANNNNTTC | 3 cut(s) | 1353, 1839, 3051 |
| MscI | TGGCCA | 1 cut(s) | 3524 |
| MseI | TTAA | 5 cut(s) | 171, 1314, 2051, 2246, 2589 |
| MslI | CAYNNNNRTG | 3 cut(s) | 2290, 3351, 3378 |
| Msp20I | TGGCCA | 1 cut(s) | 3524 |
| MspA1I | CMGCKG | 7 cut(s) | 2638, 2653, 3259, 3470, 3530, 3635, 3781 |
| MspI | CCGG | 5 cut(s) | 422, 851, 3311, 3537, 3744 |
| MspR9I | CCNGG | 1 cut(s) | 2628 |
| MunI | CAATTG | 1 cut(s) | 2043 |
| Mva1269I | GAATGC | 2 cut(s) | 1187, 2904 |
| MvaI | CCWGG | 1 cut(s) | 2628 |
| MvnI | CGCG | 2 cut(s) | 122, 2653 |
| NcoI | CCATGG | 2 cut(s) | 714, 2167 |
| NdeI | CATATG | 1 cut(s) | 3267 |
| NlaIV | GGNNCC | 4 cut(s) | 456, 591, 2364, 2657 |
| NmuCI | GTSAC | 7 cut(s) | 736, 1207, 1423, 1783, 2031, 2404, 3668 |
| NruI | TCGCGA | 1 cut(s) | 122 |
| NsiI | ATGCAT | 2 cut(s) | 577, 2512 |
| NspI | RCATGY | 3 cut(s) | 575, 1200, 2514 |
| OliI | CACNNNNGTG | 2 cut(s) | 2290, 3351 |
| PagI | TCATGA | 2 cut(s) | 337, 3376 |
| PceI | AGGCCT | 2 cut(s) | 3505, 3901 |
| PciI | ACATGT | 1 cut(s) | 1196 |
| PciSI | GCTCTTC | 1 cut(s) | 2715 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 2414 |
| PctI | GAATGC | 2 cut(s) | 1187, 2904 |
| PdmI | GAANNNNTTC | 3 cut(s) | 1353, 1839, 3051 |
| PfeI | GAWTC | 9 cut(s) | 437, 2069, 2339, 2801, 2938, 3329, 3545, 3650, 3752 |
| Pfl23II | CGTACG | 1 cut(s) | 19 |
| PflFI | GACNNNGTC | 1 cut(s) | 3870 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 74 |
| Ple19I | CGATCG | 1 cut(s) | 1018 |
| PleI | GAGTC | 6 cut(s) | 171, 339, 360, 798, 810, 3434 |
| PpsI | GAGTC | 6 cut(s) | 171, 339, 360, 798, 810, 3434 |
| PscI | ACATGT | 1 cut(s) | 1196 |
| PshAI | GACNNNNGTC | 1 cut(s) | 1248 |
| Psp6I | CCWGG | 1 cut(s) | 2626 |
| PspFI | CCCAGC | 3 cut(s) | 484, 2464, 3171 |
| PspGI | CCWGG | 1 cut(s) | 2626 |
| PspLI | CGTACG | 1 cut(s) | 19 |
| PspN4I | GGNNCC | 4 cut(s) | 456, 591, 2364, 2657 |
| PspPI | GGNCC | 1 cut(s) | 3065 |
| PstNI | CAGNNNCTG | 1 cut(s) | 254 |
| PsuI | RGATCY | 5 cut(s) | 845, 1499, 1706, 2822, 2891 |
| PsyI | GACNNNGTC | 1 cut(s) | 3870 |
| PvuI | CGATCG | 1 cut(s) | 1018 |
| PvuII | CAGCTG | 5 cut(s) | 3259, 3470, 3530, 3635, 3781 |
| RruI | TCGCGA | 1 cut(s) | 122 |
| RsaI | GTAC | 5 cut(s) | 21, 723, 2869, 3715, 3823 |
| RsaNI | GTAC | 5 cut(s) | 20, 722, 2868, 3714, 3822 |
| RseI | CAYNNNNRTG | 3 cut(s) | 2290, 3351, 3378 |
| SacII | CCGCGG | 1 cut(s) | 2654 |
| SapI | GCTCTTC | 1 cut(s) | 2715 |
| SaqAI | TTAA | 5 cut(s) | 171, 1314, 2051, 2246, 2589 |
| Sau96I | GGNCC | 1 cut(s) | 3065 |
| SchI | GAGTC | 6 cut(s) | 172, 339, 361, 799, 811, 3434 |
| ScrFI | CCNGG | 1 cut(s) | 2628 |
| SduI | GDGCHC | 2 cut(s) | 2428, 2978 |
| SfcI | CTRYAG | 3 cut(s) | 3240, 3435, 3630 |
| Sfr303I | CCGCGG | 1 cut(s) | 2654 |
| SgrBI | CCGCGG | 1 cut(s) | 2654 |
| SinI | GGWCC | 1 cut(s) | 3065 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 2290, 3351, 3378 |
| SmlI | CTYRAG | 4 cut(s) | 349, 2134, 2522, 3206 |
| SmoI | CTYRAG | 4 cut(s) | 349, 2134, 2522, 3206 |
| SpeI | ACTAGT | 1 cut(s) | 3703 |
| SseBI | AGGCCT | 2 cut(s) | 3505, 3901 |
| SsiI | CCGC | 9 cut(s) | 381, 695, 2565, 2638, 2651, 2653, 2971, 3482, 3802 |
| SspMI | CTAG | 9 cut(s) | 99, 806, 1061, 1256, 1955, 2541, 2826, 3062, 3704 |
| StuI | AGGCCT | 2 cut(s) | 3505, 3901 |
| StyD4I | CCNGG | 1 cut(s) | 2626 |
| StyI | CCWWGG | 5 cut(s) | 714, 2167, 2540, 3001, 3061 |
| TaaI | ACNGT | 8 cut(s) | 323, 410, 1875, 1917, 2885, 3287, 3436, 3835 |
| TaiI | ACGT | 5 cut(s) | 598, 639, 1657, 2675, 3568 |
| TaqI | TCGA | 9 cut(s) | 278, 1018, 1485, 1759, 2422, 2782, 2896, 3886, 3904 |
| TatI | WGTACW | 2 cut(s) | 721, 2867 |
| TauI | GCSGC | 3 cut(s) | 2641, 2656, 3484 |
| TfiI | GAWTC | 9 cut(s) | 437, 2069, 2339, 2801, 2938, 3329, 3545, 3650, 3752 |
| Tru1I | TTAA | 5 cut(s) | 171, 1314, 2051, 2246, 2589 |
| Tru9I | TTAA | 5 cut(s) | 171, 1314, 2051, 2246, 2589 |
| TscAI | CASTG | 9 cut(s) | 1365, 1387, 1432, 1572, 1788, 1880, 2890, 3049, 3838 |
| TseFI | GTSAC | 7 cut(s) | 736, 1207, 1423, 1783, 2031, 2404, 3668 |
| Tsp45I | GTSAC | 7 cut(s) | 736, 1207, 1423, 1783, 2031, 2404, 3668 |
| TspGWI | ACGGA | 3 cut(s) | 7, 53, 2423 |
| TspRI | CASTG | 9 cut(s) | 1365, 1387, 1432, 1572, 1788, 1880, 2890, 3049, 3838 |
| Tth111I | GACNNNGTC | 1 cut(s) | 3870 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 74 |
| VneI | GTGCAC | 1 cut(s) | 2974 |
| VpaK11BI | GGWCC | 1 cut(s) | 3065 |
| XapI | RAATTY | 6 cut(s) | 299, 897, 1839, 1944, 3227, 3767 |
| XbaI | TCTAGA | 1 cut(s) | 805 |
| XceI | RCATGY | 3 cut(s) | 575, 1200, 2514 |
| XmaJI | CCTAGG | 2 cut(s) | 2540, 3061 |
| XmiI | GTMKAC | 3 cut(s) | 1252, 2551, 3438 |
| XmnI | GAANNNNTTC | 3 cut(s) | 1353, 1839, 3051 |
| XspI | CTAG | 9 cut(s) | 99, 806, 1061, 1256, 1955, 2541, 2826, 3062, 3704 |
| Zsp2I | ATGCAT | 2 cut(s) | 577, 2512 |