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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 234 |
| AclWI | GGATC | 2 cut(s) | 214, 370 |
| AgsI | TTSAA | 2 cut(s) | 128, 296 |
| AluBI | AGCT | 1 cut(s) | 95 |
| AluI | AGCT | 1 cut(s) | 95 |
| AlwI | GGATC | 2 cut(s) | 214, 370 |
| ApeKI | GCWGC | 1 cut(s) | 260 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 123 |
| AsuHPI | GGTGA | 2 cut(s) | 32, 328 |
| BbsI | GAAGAC | 1 cut(s) | 68 |
| BbvI | GCAGC | 1 cut(s) | 247 |
| BccI | CCATC | 1 cut(s) | 387 |
| BfaI | CTAG | 1 cut(s) | 33 |
| BisI | GCNGC | 1 cut(s) | 261 |
| BlsI | GCNGC | 1 cut(s) | 262 |
| BmsI | GCATC | 1 cut(s) | 334 |
| BpiI | GAAGAC | 1 cut(s) | 68 |
| BpmI | CTGGAG | 1 cut(s) | 195 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 193, 223 |
| Bse1I | ACTGG | 1 cut(s) | 208 |
| BseNI | ACTGG | 1 cut(s) | 208 |
| BseXI | GCAGC | 1 cut(s) | 247 |
| BslFI | GGGAC | 1 cut(s) | 32 |
| BsmFI | GGGAC | 1 cut(s) | 32 |
| Bsp143I | GATC | 4 cut(s) | 206, 301, 362, 418 |
| BspHI | TCATGA | 1 cut(s) | 372 |
| BspPI | GGATC | 2 cut(s) | 214, 370 |
| BsrI | ACTGG | 1 cut(s) | 208 |
| BssMI | GATC | 4 cut(s) | 206, 301, 362, 418 |
| Bst4CI | ACNGT | 1 cut(s) | 177 |
| BstKTI | GATC | 4 cut(s) | 209, 304, 365, 421 |
| BstMBI | GATC | 4 cut(s) | 206, 301, 362, 418 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 157, 266 |
| BstV1I | GCAGC | 1 cut(s) | 247 |
| BstV2I | GAAGAC | 1 cut(s) | 68 |
| BstX2I | RGATCY | 2 cut(s) | 206, 362 |
| BstYI | RGATCY | 2 cut(s) | 206, 362 |
| CciI | TCATGA | 1 cut(s) | 372 |
| CviAII | CATG | 2 cut(s) | 373, 450 |
| CviJI | RGCY | 2 cut(s) | 95, 378 |
| CviKI_1 | RGCY | 2 cut(s) | 95, 378 |
| DpnI | GATC | 4 cut(s) | 208, 303, 364, 420 |
| DpnII | GATC | 4 cut(s) | 206, 301, 362, 418 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 234 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 234 |
| FaeI | CATG | 2 cut(s) | 376, 453 |
| FaiI | YATR | 3 cut(s) | 374, 430, 451 |
| FaqI | GGGAC | 1 cut(s) | 32 |
| FatI | CATG | 2 cut(s) | 372, 449 |
| Fnu4HI | GCNGC | 1 cut(s) | 261 |
| Fsp4HI | GCNGC | 1 cut(s) | 261 |
| FspBI | CTAG | 1 cut(s) | 33 |
| GluI | GCNGC | 1 cut(s) | 261 |
| GsuI | CTGGAG | 1 cut(s) | 195 |
| Hin1II | CATG | 2 cut(s) | 376, 453 |
| HincII | GTYRAC | 1 cut(s) | 193 |
| HindII | GTYRAC | 1 cut(s) | 193 |
| HinfI | GANTC | 4 cut(s) | 23, 64, 335, 392 |
| HphI | GGTGA | 2 cut(s) | 32, 328 |
| Hpy166II | GTNNAC | 1 cut(s) | 193 |
| Hpy188I | TCNGA | 2 cut(s) | 69, 367 |
| Hpy188III | TCNNGA | 8 cut(s) | 33, 49, 212, 284, 341, 373, 412, 424 |
| Hpy8I | GTNNAC | 1 cut(s) | 193 |
| HpyAV | CCTTC | 2 cut(s) | 64, 353 |
| HpyCH4III | ACNGT | 1 cut(s) | 177 |
| HpyCH4V | TGCA | 3 cut(s) | 263, 347, 432 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 157, 266 |
| Hsp92II | CATG | 2 cut(s) | 376, 453 |
| Kzo9I | GATC | 4 cut(s) | 206, 301, 362, 418 |
| LpnPI | CCDG | 4 cut(s) | 137, 189, 225, 354 |
| Lsp1109I | GCAGC | 1 cut(s) | 247 |
| LweI | GCATC | 1 cut(s) | 334 |
| MaeI | CTAG | 1 cut(s) | 33 |
| MalI | GATC | 4 cut(s) | 208, 303, 364, 420 |
| MboI | GATC | 4 cut(s) | 206, 301, 362, 418 |
| MboII | GAAGA | 6 cut(s) | 48, 73, 128, 263, 308, 311 |
| MfeI | CAATTG | 1 cut(s) | 264 |
| MflI | RGATCY | 2 cut(s) | 206, 362 |
| MluCI | AATT | 3 cut(s) | 107, 264, 291 |
| MlyI | GAGTC | 2 cut(s) | 58, 401 |
| MmeI | TCCRAC | 1 cut(s) | 32 |
| MnlI | CCTC | 1 cut(s) | 419 |
| MroXI | GAANNNNTTC | 1 cut(s) | 123 |
| MunI | CAATTG | 1 cut(s) | 264 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 157, 266 |
| NdeII | GATC | 4 cut(s) | 206, 301, 362, 418 |
| NlaIII | CATG | 2 cut(s) | 376, 453 |
| NmeAIII | GCCGAG | 1 cut(s) | 415 |
| PagI | TCATGA | 1 cut(s) | 372 |
| PdmI | GAANNNNTTC | 1 cut(s) | 123 |
| PfeI | GAWTC | 2 cut(s) | 23, 335 |
| PflFI | GACNNNGTC | 1 cut(s) | 234 |
| PkrI | GCNGC | 1 cut(s) | 262 |
| PleI | GAGTC | 2 cut(s) | 58, 400 |
| PpsI | GAGTC | 2 cut(s) | 58, 400 |
| PsuI | RGATCY | 2 cut(s) | 206, 362 |
| PsyI | GACNNNGTC | 1 cut(s) | 234 |
| SatI | GCNGC | 1 cut(s) | 261 |
| Sau3AI | GATC | 4 cut(s) | 206, 301, 362, 418 |
| SchI | GAGTC | 2 cut(s) | 58, 401 |
| SetI | ASST | 4 cut(s) | 22, 97, 318, 437 |
| SfaNI | GCATC | 1 cut(s) | 334 |
| Sse9I | AATT | 3 cut(s) | 107, 264, 291 |
| SspMI | CTAG | 1 cut(s) | 33 |
| TaaI | ACNGT | 1 cut(s) | 177 |
| TasI | AATT | 3 cut(s) | 107, 264, 291 |
| TfiI | GAWTC | 2 cut(s) | 23, 335 |
| TseI | GCWGC | 1 cut(s) | 260 |
| TspDTI | ATGAA | 1 cut(s) | 120 |
| Tth111I | GACNNNGTC | 1 cut(s) | 234 |
| XbaI | TCTAGA | 1 cut(s) | 32 |
| XmnI | GAANNNNTTC | 1 cut(s) | 123 |
| XspI | CTAG | 1 cut(s) | 33 |