RLG00000003323

Domain in histone families 1 and 5

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0000827
Physical Location & Seq
Reverse (-)
47062306 .. 47065587
3282 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000003323

Sequence Viewer

Length: 1398 bp
ATGATAAACAAGCTTGCTGGTGCTGTGATGGAGAAGCTCGCATCCCCTCCAAAACACACACACCGGATCAGAAGAATGACGATACATAGGGCAATTGAGGAGTTGACTGAGGAACAGGGGTCAACTGAAGAAGCAATATCAAAATTTATTAGAGAGAAGTTTGACTGTTTGCCATTGGCTCATGCAAGTTTCTTGAGTCTTCACTTGAAGAAGCTAAACGAGTGGGGAGAAATTGTTTGCGTATCAAAAAATTTTTATATGCTTCCAACTGATAAAGGTGATTCTCTTTCTAGAAGAAAGTGTGTCCCAAAACAAAAGAAGACTCGGAAGGGTTGGGGAAGAAGAAAATCAGCTAAAGGAAATGAACATAAAACATTAGCAGAAGAACAAGTTGAAGTGACTGAGAAACAGAGAAACCAGGGGAAGCAAAGTCAAAAACCACAGTATTGTAAGGCAGTTGACAACCAAACTGGATCTCCAGAGCAAGTCAATGGACGACAATGTCAAAGACAAATGGAAGAAATCGCTGCAATTGCTGAAAAGAAAAGTCAAGAACAAATTGGAGAAGATCAGTTGAGTAAGGTGATTGATGATAAAAAAGAATCTCCTGATGCACAGCAAGGAGAAAGATGGAGCTATCCCCGGTCTCACTTGACAATTGAAGGAAACGTCCCTAAAGGAACTATCACCCAATCATTTGGAGCAGGAGAGAATGAGTGTTCAATAATCCTTCTGTGGACTAATGTATTTGCTTTGGTATCACCTACTTTATGGTCTCCCTTCTTCATGCCGTTTGTGGCCAGGCAAGTTCCAGGTTCAACCACGTATAGTCTAGCGCTATACTATATGATGAGTTCTCTGGTTGAGGATGCACCCTTGCTGAGAATTTTGTTAACGGGGAAACTTATATCATCTCTATCTCCCTTTTTAGAGAATTTTTTAAAAACAGTGGGAGTGGGTTGCTATAAGGAGTGCCTACAATACGAAAGCAAGCATTCAAGCCTGCAGCAAGCAAACAATATAGCGGCATCATTGGTCAAGCAAACATTGGCCACTGCAAATGTTAAAGAGTTTCGTGGCTGTATAACACGTGCTGCAGCTGCTTCATTAGATACATCCAGTCAGGTTCACCCATTAAAATTAAGCGCACAACCCACGAAGCAGAGTCAGAATCTGGATTTGCGAGCAAATCCAAAGAGAGTTTCTGTGGACGAGAACAACAGGAATGAGGCTGGAAATGCATGCGTCCGGCATAAGAGGAGGGCAGCGCTTCAGGATGCAACCGATGCTTTTTGTAAACCTTCATATGGGGACTGCTTCAATGCAACTAAACTTCAGGTCAATATGCTATTAATTAAGTTCTTCATAATACAAATTCTGGTATTCTCATCAATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Linker_histone

Protein Analysis

466

Amino Acids

52.17

Weight (kDa)

9.29

Isoelectric Point (pI)

58.16

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 501
AciI CCGC 1 cut(s) 1025
AclWI GGATC 2 cut(s) 74, 481
AcoI YGGCCR 2 cut(s) 798, 1050
AcsI RAATTY 5 cut(s) 143, 250, 885, 934, 1374
AcuI CTGAAG 3 cut(s) 147, 1256, 1319
AcvI CACGTG 1 cut(s) 1091
AfeI AGCGCT 2 cut(s) 837, 1269
AfiI CCNNNNNNNGG 1 cut(s) 1307
AflIII ACRYGT 1 cut(s) 1088
AgsI TTSAA 7 cut(s) 208, 395, 662, 723, 819, 999, 1321
AjnI CCWGG 3 cut(s) 417, 800, 811
AjuI GAANNNNNNNTTGG 2 cut(s) 1186, 1218
AluBI AGCT 6 cut(s) 13, 37, 214, 353, 636, 1100
AluI AGCT 6 cut(s) 13, 37, 214, 353, 636, 1100
Alw26I GTCTC 2 cut(s) 651, 780
AlwI GGATC 2 cut(s) 74, 481
AlwNI CAGNNNCTG 1 cut(s) 1174
Aor51HI AGCGCT 2 cut(s) 837, 1269
AoxI GGCC 2 cut(s) 798, 1050
ApeKI GCWGC 6 cut(s) 527, 1006, 1094, 1097, 1100, 1265
ApoI RAATTY 5 cut(s) 143, 250, 885, 934, 1374
AseI ATTAAT 1 cut(s) 1352
AspLEI GCGC 3 cut(s) 838, 1148, 1270
AsuC2I CCSGG 1 cut(s) 643
AsuHPI GGTGA 5 cut(s) 290, 595, 679, 753, 1121
BalI TGGCCA 2 cut(s) 800, 1052
BbrPI CACGTG 1 cut(s) 1091
BbsI GAAGAC 2 cut(s) 191, 326
BbvI GCAGC 6 cut(s) 514, 1018, 1081, 1087, 1109, 1277
BccI CCATC 2 cut(s) 22, 624
BceAI ACGGC 1 cut(s) 775
BciT130I CCWGG 3 cut(s) 419, 802, 813
BcnI CCSGG 1 cut(s) 643
BcoDI GTCTC 2 cut(s) 651, 780
BfaI CTAG 2 cut(s) 291, 833
BfmI CTRYAG 2 cut(s) 1004, 1095
BfoI RGCGCY 2 cut(s) 839, 1271
BisI GCNGC 7 cut(s) 528, 1007, 1026, 1095, 1098, 1101, 1266
BlsI GCNGC 7 cut(s) 529, 1008, 1027, 1096, 1099, 1102, 1267
Bme1390I CCNGG 4 cut(s) 419, 643, 802, 813
BmrFI CCNGG 4 cut(s) 419, 643, 802, 813
BmsI GCATC 6 cut(s) 50, 601, 859, 1037, 1267, 1276
BpiI GAAGAC 2 cut(s) 191, 326
BpmI CTGGAG 1 cut(s) 462
BpuEI CTTGAG 1 cut(s) 214
BpuMI CCSGG 1 cut(s) 643
BsaAI YACGTR 2 cut(s) 825, 1091
BsaI GGTCTC 2 cut(s) 651, 780
BsaJI CCNNGG 2 cut(s) 418, 641
BsaWI WCCGGW 1 cut(s) 63
BsaXI ACNNNNNCTCC 4 cut(s) 219, 249, 460, 490
Bsc4I CCNNNNNNNGG 1 cut(s) 1307
Bse1I ACTGG 2 cut(s) 475, 1119
BseBI CCWGG 3 cut(s) 419, 802, 813
BseDI CCNNGG 2 cut(s) 418, 641
BseGI GGATG 4 cut(s) 41, 874, 1115, 1282
BseLI CCNNNNNNNGG 1 cut(s) 1307
BseMII CTCAG 3 cut(s) 99, 393, 872
BseNI ACTGG 2 cut(s) 475, 1119
BseRI GAGGAG 2 cut(s) 113, 1273
BseXI GCAGC 6 cut(s) 514, 1018, 1081, 1087, 1109, 1277
BshFI GGCC 2 cut(s) 800, 1052
BsiSI CCGG 3 cut(s) 64, 643, 1249
BslFI GGGAC 3 cut(s) 290, 656, 1325
BslI CCNNNNNNNGG 1 cut(s) 1307
BsmAI GTCTC 2 cut(s) 651, 780
BsmFI GGGAC 3 cut(s) 290, 656, 1325
BsmI GAATGC 1 cut(s) 994
BsnI GGCC 2 cut(s) 800, 1052
Bso31I GGTCTC 2 cut(s) 651, 780
Bsp143I GATC 3 cut(s) 66, 473, 568
BspACI CCGC 1 cut(s) 1025
BspANI GGCC 2 cut(s) 800, 1052
BspCNI CTCAG 3 cut(s) 100, 394, 873
BspMAI CTGCAG 2 cut(s) 1008, 1099
BspPI GGATC 2 cut(s) 74, 481
BspTNI GGTCTC 2 cut(s) 651, 780
BsrI ACTGG 2 cut(s) 475, 1119
BssECI CCNNGG 2 cut(s) 418, 641
BssMI GATC 3 cut(s) 66, 473, 568
Bst2UI CCWGG 3 cut(s) 419, 802, 813
Bst4CI ACNGT 3 cut(s) 167, 444, 949
BstAPI GCANNNNNTGC 1 cut(s) 1286
BstBAI YACGTR 2 cut(s) 825, 1091
BstC8I GCNNGC 7 cut(s) 15, 39, 992, 1004, 1011, 1185, 1243
BstDEI CTNAG 3 cut(s) 108, 402, 881
BstF5I GGATG 4 cut(s) 41, 874, 1115, 1282
BstH2I RGCGCY 2 cut(s) 839, 1271
BstHHI GCGC 3 cut(s) 838, 1148, 1270
BstKTI GATC 3 cut(s) 69, 476, 571
BstMAI GTCTC 2 cut(s) 651, 780
BstMBI GATC 3 cut(s) 66, 473, 568
BstMWI GCNNNNNNNGC 4 cut(s) 533, 1100, 1238, 1286
BstNI CCWGG 3 cut(s) 419, 802, 813
BstNSI RCATGY 1 cut(s) 1245
BstSCI CCNGG 4 cut(s) 417, 641, 800, 811
BstSFI CTRYAG 2 cut(s) 1004, 1095
BstV1I GCAGC 6 cut(s) 514, 1018, 1081, 1087, 1109, 1277
BstV2I GAAGAC 2 cut(s) 191, 326
BstX2I RGATCY 1 cut(s) 473
BstXI CCANNNNNNTGG 1 cut(s) 698
BstYI RGATCY 1 cut(s) 473
BsuRI GGCC 2 cut(s) 800, 1052
BtsCI GGATG 4 cut(s) 41, 874, 1115, 1282
BtsI GCAGTG 1 cut(s) 1053
BtsIMutI CAGTG 2 cut(s) 954, 1053
Cac8I GCNNGC 7 cut(s) 15, 39, 992, 1004, 1011, 1185, 1243
CaiI CAGNNNCTG 1 cut(s) 1174
CfoI GCGC 3 cut(s) 838, 1148, 1270
CseI GACGC 1 cut(s) 1234
CviAII CATG 3 cut(s) 182, 787, 1242
DdeI CTNAG 3 cut(s) 108, 402, 881
DpnI GATC 3 cut(s) 68, 475, 570
DpnII GATC 3 cut(s) 66, 473, 568
DraI TTTAAA 1 cut(s) 942
DrdI GACNNNNNNGTC 1 cut(s) 501
DseDI GACNNNNNNGTC 1 cut(s) 501
EaeI YGGCCR 2 cut(s) 798, 1050
Eco31I GGTCTC 2 cut(s) 651, 780
Eco47III AGCGCT 2 cut(s) 837, 1269
Eco57I CTGAAG 3 cut(s) 147, 1256, 1319
Eco72I CACGTG 1 cut(s) 1091
EcoRII CCWGG 3 cut(s) 417, 800, 811
EcoT22I ATGCAT 1 cut(s) 1243
FaeI CATG 3 cut(s) 185, 790, 1245
FaqI GGGAC 3 cut(s) 290, 656, 1325
FatI CATG 3 cut(s) 181, 786, 1241
FauNDI CATATG 1 cut(s) 1306
Fnu4HI GCNGC 7 cut(s) 528, 1007, 1026, 1095, 1098, 1101, 1266
FokI GGATG 4 cut(s) 28, 881, 1102, 1289
Fsp4HI GCNGC 7 cut(s) 528, 1007, 1026, 1095, 1098, 1101, 1266
FspBI CTAG 2 cut(s) 291, 833
GlaI GCGC 3 cut(s) 837, 1147, 1269
GluI GCNGC 7 cut(s) 528, 1007, 1026, 1095, 1098, 1101, 1266
GsuI CTGGAG 1 cut(s) 462
HaeII RGCGCY 2 cut(s) 839, 1271
HaeIII GGCC 2 cut(s) 800, 1052
HapII CCGG 3 cut(s) 64, 643, 1249
HgaI GACGC 1 cut(s) 1234
HhaI GCGC 3 cut(s) 838, 1148, 1270
Hin1II CATG 3 cut(s) 185, 790, 1245
Hin6I GCGC 3 cut(s) 836, 1146, 1268
HinP1I GCGC 3 cut(s) 836, 1146, 1268
HincII GTYRAC 4 cut(s) 105, 123, 460, 894
HindII GTYRAC 4 cut(s) 105, 123, 460, 894
HindIII AAGCTT 1 cut(s) 11
HinfI GANTC 6 cut(s) 196, 281, 322, 602, 1165, 1171
HpaI GTTAAC 1 cut(s) 894
HpaII CCGG 3 cut(s) 64, 643, 1249
HphI GGTGA 5 cut(s) 290, 595, 679, 753, 1121
Hpy166II GTNNAC 8 cut(s) 105, 123, 460, 738, 894, 1129, 1210, 1298
Hpy188I TCNGA 3 cut(s) 71, 327, 1170
Hpy188III TCNNGA 7 cut(s) 193, 291, 479, 551, 608, 1175, 1274
Hpy8I GTNNAC 8 cut(s) 105, 123, 460, 738, 894, 1129, 1210, 1298
HpyAV CCTTC 5 cut(s) 322, 656, 740, 790, 1311
HpyCH4III ACNGT 3 cut(s) 167, 444, 949
HpyCH4IV ACGT 3 cut(s) 669, 824, 1090
HpyF10VI GCNNNNNNNGC 4 cut(s) 533, 1100, 1238, 1286
HpyF3I CTNAG 3 cut(s) 108, 402, 881
HpySE526I ACGT 3 cut(s) 669, 824, 1090
Hsp92II CATG 3 cut(s) 185, 790, 1245
HspAI GCGC 3 cut(s) 836, 1146, 1268
KspAI GTTAAC 1 cut(s) 894
Kzo9I GATC 3 cut(s) 66, 473, 568
LmnI GCTCC 2 cut(s) 633, 701
Lsp1109I GCAGC 6 cut(s) 514, 1018, 1081, 1087, 1109, 1277
LweI GCATC 6 cut(s) 50, 601, 859, 1037, 1267, 1276
MaeI CTAG 2 cut(s) 291, 833
MaeII ACGT 3 cut(s) 669, 824, 1090
MaeIII GTNAC 1 cut(s) 397
MalI GATC 3 cut(s) 68, 475, 570
MboI GATC 3 cut(s) 66, 473, 568
MfeI CAATTG 3 cut(s) 93, 531, 657
MflI RGATCY 1 cut(s) 473
MlsI TGGCCA 2 cut(s) 800, 1052
MluNI TGGCCA 2 cut(s) 800, 1052
MlyI GAGTC 3 cut(s) 205, 316, 1174
MmeI TCCRAC 1 cut(s) 290
MnlI CCTC 7 cut(s) 57, 91, 103, 859, 1222, 1251, 1254
Mox20I TGGCCA 2 cut(s) 800, 1052
Mph1103I ATGCAT 1 cut(s) 1243
MscI TGGCCA 2 cut(s) 800, 1052
MseI TTAA 7 cut(s) 893, 941, 1065, 1136, 1142, 1352, 1356
Msp20I TGGCCA 2 cut(s) 800, 1052
MspA1I CMGCKG 1 cut(s) 1100
MspI CCGG 3 cut(s) 64, 643, 1249
MspR9I CCNGG 4 cut(s) 419, 643, 802, 813
MunI CAATTG 3 cut(s) 93, 531, 657
Mva1269I GAATGC 1 cut(s) 994
MvaI CCWGG 3 cut(s) 419, 802, 813
MwoI GCNNNNNNNGC 4 cut(s) 533, 1100, 1238, 1286
NciI CCSGG 1 cut(s) 643
NdeI CATATG 1 cut(s) 1306
NdeII GATC 3 cut(s) 66, 473, 568
NlaIII CATG 3 cut(s) 185, 790, 1245
NmuCI GTSAC 1 cut(s) 397
NsiI ATGCAT 1 cut(s) 1243
NspI RCATGY 1 cut(s) 1245
PacI TTAATTAA 1 cut(s) 1356
PaeI GCATGC 1 cut(s) 1245
PctI GAATGC 1 cut(s) 994
PfeI GAWTC 3 cut(s) 281, 602, 1171
PflFI GACNNNGTC 1 cut(s) 501
PkrI GCNGC 7 cut(s) 529, 1008, 1027, 1096, 1099, 1102, 1267
PleI GAGTC 3 cut(s) 204, 316, 1173
PmaCI CACGTG 1 cut(s) 1091
PmlI CACGTG 1 cut(s) 1091
PpsI GAGTC 3 cut(s) 204, 316, 1173
Ppu21I YACGTR 2 cut(s) 825, 1091
PshBI ATTAAT 1 cut(s) 1352
Psp6I CCWGG 3 cut(s) 417, 800, 811
PspCI CACGTG 1 cut(s) 1091
PspGI CCWGG 3 cut(s) 417, 800, 811
PstI CTGCAG 2 cut(s) 1008, 1099
PstNI CAGNNNCTG 1 cut(s) 1174
PsuI RGATCY 1 cut(s) 473
PsyI GACNNNGTC 1 cut(s) 501
PvuII CAGCTG 1 cut(s) 1100
SaqAI TTAA 7 cut(s) 893, 941, 1065, 1136, 1142, 1352, 1356
SatI GCNGC 7 cut(s) 528, 1007, 1026, 1095, 1098, 1101, 1266
Sau3AI GATC 3 cut(s) 66, 473, 568
SchI GAGTC 3 cut(s) 205, 316, 1174
ScrFI CCNGG 4 cut(s) 419, 643, 802, 813
SfaNI GCATC 6 cut(s) 50, 601, 859, 1037, 1267, 1276
SfcI CTRYAG 2 cut(s) 1004, 1095
SmlI CTYRAG 1 cut(s) 193
SmoI CTYRAG 1 cut(s) 193
SphI GCATGC 1 cut(s) 1245
SsiI CCGC 1 cut(s) 1025
SspMI CTAG 2 cut(s) 291, 833
StyD4I CCNGG 4 cut(s) 417, 641, 800, 811
TaaI ACNGT 3 cut(s) 167, 444, 949
TaiI ACGT 3 cut(s) 672, 827, 1093
TauI GCSGC 1 cut(s) 1028
TfiI GAWTC 3 cut(s) 281, 602, 1171
Tru1I TTAA 7 cut(s) 893, 941, 1065, 1136, 1142, 1352, 1356
Tru9I TTAA 7 cut(s) 893, 941, 1065, 1136, 1142, 1352, 1356
TscAI CASTG 2 cut(s) 954, 1060
TseFI GTSAC 1 cut(s) 397
TseI GCWGC 6 cut(s) 527, 1006, 1094, 1097, 1100, 1265
Tsp45I GTSAC 1 cut(s) 397
TspDTI ATGAA 5 cut(s) 378, 775, 1095, 1293, 1354
TspRI CASTG 2 cut(s) 954, 1060
Tth111I GACNNNGTC 1 cut(s) 501
VspI ATTAAT 1 cut(s) 1352
XapI RAATTY 5 cut(s) 143, 250, 885, 934, 1374
XbaI TCTAGA 1 cut(s) 290
XceI RCATGY 1 cut(s) 1245
XspI CTAG 2 cut(s) 291, 833
Zsp2I ATGCAT 1 cut(s) 1243
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.