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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccIII | TCCGGA | 1 cut(s) | 227 |
| AciI | CCGC | 1 cut(s) | 8 |
| AcuI | CTGAAG | 1 cut(s) | 273 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 234 |
| AflIII | ACRYGT | 1 cut(s) | 22 |
| AgeI | ACCGGT | 1 cut(s) | 73 |
| AgsI | TTSAA | 2 cut(s) | 20, 269 |
| AluBI | AGCT | 1 cut(s) | 166 |
| AluI | AGCT | 1 cut(s) | 166 |
| Alw26I | GTCTC | 1 cut(s) | 242 |
| Aor13HI | TCCGGA | 1 cut(s) | 227 |
| AsiGI | ACCGGT | 1 cut(s) | 73 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 75 |
| BclI | TGATCA | 1 cut(s) | 109 |
| BcoDI | GTCTC | 1 cut(s) | 242 |
| BsaWI | WCCGGW | 2 cut(s) | 73, 227 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 234 |
| Bse118I | RCCGGY | 1 cut(s) | 73 |
| Bse3DI | GCAATG | 1 cut(s) | 188 |
| BseAI | TCCGGA | 1 cut(s) | 227 |
| BseGI | GGATG | 2 cut(s) | 125, 224 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 234 |
| BseMI | GCAATG | 1 cut(s) | 188 |
| BseRI | GAGGAG | 1 cut(s) | 146 |
| BshTI | ACCGGT | 1 cut(s) | 73 |
| BsiSI | CCGG | 2 cut(s) | 74, 228 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 234 |
| BsmAI | GTCTC | 1 cut(s) | 242 |
| Bsp13I | TCCGGA | 1 cut(s) | 227 |
| Bsp143I | GATC | 1 cut(s) | 109 |
| BspACI | CCGC | 1 cut(s) | 8 |
| BspEI | TCCGGA | 1 cut(s) | 227 |
| BsrDI | GCAATG | 1 cut(s) | 188 |
| BsrFI | RCCGGY | 1 cut(s) | 73 |
| BssAI | RCCGGY | 1 cut(s) | 73 |
| BssMI | GATC | 1 cut(s) | 109 |
| Bst6I | CTCTTC | 1 cut(s) | 240 |
| BstF5I | GGATG | 2 cut(s) | 125, 224 |
| BstKTI | GATC | 1 cut(s) | 112 |
| BstMAI | GTCTC | 1 cut(s) | 242 |
| BstMBI | GATC | 1 cut(s) | 109 |
| BstNSI | RCATGY | 1 cut(s) | 26 |
| BtsCI | GGATG | 2 cut(s) | 125, 224 |
| BtsI | GCAGTG | 1 cut(s) | 147 |
| BtsIMutI | CAGTG | 1 cut(s) | 147 |
| Cfr10I | RCCGGY | 1 cut(s) | 73 |
| CspAI | ACCGGT | 1 cut(s) | 73 |
| CviAII | CATG | 1 cut(s) | 23 |
| CviJI | RGCY | 1 cut(s) | 166 |
| CviKI_1 | RGCY | 1 cut(s) | 166 |
| DpnI | GATC | 1 cut(s) | 111 |
| DpnII | GATC | 1 cut(s) | 109 |
| Eam1104I | CTCTTC | 1 cut(s) | 240 |
| EarI | CTCTTC | 1 cut(s) | 240 |
| Eco57I | CTGAAG | 1 cut(s) | 273 |
| FaeI | CATG | 1 cut(s) | 26 |
| FaiI | YATR | 1 cut(s) | 24 |
| FatI | CATG | 1 cut(s) | 22 |
| FbaI | TGATCA | 1 cut(s) | 109 |
| FokI | GGATG | 2 cut(s) | 132, 211 |
| HapII | CCGG | 2 cut(s) | 74, 228 |
| Hin1II | CATG | 1 cut(s) | 26 |
| HpaII | CCGG | 2 cut(s) | 74, 228 |
| Hpy188I | TCNGA | 1 cut(s) | 114 |
| Hpy188III | TCNNGA | 3 cut(s) | 80, 188, 228 |
| HpyAV | CCTTC | 1 cut(s) | 227 |
| HpyCH4V | TGCA | 1 cut(s) | 140 |
| Hsp92II | CATG | 1 cut(s) | 26 |
| Kpn2I | TCCGGA | 1 cut(s) | 227 |
| Ksp22I | TGATCA | 1 cut(s) | 109 |
| Kzo9I | GATC | 1 cut(s) | 109 |
| LpnPI | CCDG | 4 cut(s) | 87, 138, 164, 241 |
| MaeIII | GTNAC | 1 cut(s) | 271 |
| MalI | GATC | 1 cut(s) | 111 |
| MboI | GATC | 1 cut(s) | 109 |
| MboII | GAAGA | 3 cut(s) | 50, 257, 266 |
| MluCI | AATT | 1 cut(s) | 29 |
| MnlI | CCTC | 1 cut(s) | 124 |
| MroI | TCCGGA | 1 cut(s) | 227 |
| MroXI | GAANNNNTTC | 1 cut(s) | 75 |
| MspI | CCGG | 2 cut(s) | 74, 228 |
| NdeII | GATC | 1 cut(s) | 109 |
| NlaIII | CATG | 1 cut(s) | 26 |
| NmuCI | GTSAC | 1 cut(s) | 271 |
| NspI | RCATGY | 1 cut(s) | 26 |
| PciI | ACATGT | 1 cut(s) | 22 |
| PdmI | GAANNNNTTC | 1 cut(s) | 75 |
| PinAI | ACCGGT | 1 cut(s) | 73 |
| PscI | ACATGT | 1 cut(s) | 22 |
| PsrI | GAACNNNNNNTAC | 1 cut(s) | 270 |
| Sau3AI | GATC | 1 cut(s) | 109 |
| SetI | ASST | 3 cut(s) | 102, 153, 168 |
| Sse9I | AATT | 1 cut(s) | 29 |
| SsiI | CCGC | 1 cut(s) | 8 |
| TaqI | TCGA | 1 cut(s) | 79 |
| TasI | AATT | 1 cut(s) | 29 |
| TscAI | CASTG | 1 cut(s) | 147 |
| TseFI | GTSAC | 1 cut(s) | 271 |
| Tsp45I | GTSAC | 1 cut(s) | 271 |
| TspDTI | ATGAA | 1 cut(s) | 63 |
| TspRI | CASTG | 1 cut(s) | 147 |
| XceI | RCATGY | 1 cut(s) | 26 |
| XmnI | GAANNNNTTC | 1 cut(s) | 75 |