pycom15g20140

L-type lectin-domain containing receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0000688
Physical Location & Seq
Reverse (-)
14381390 .. 14385115
3726 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g20140.1

Sequence Viewer

Length: 2181 bp
ATGAGCGAGCGTGCAACGACGTCGTCGTCTTCTTCTTCCAGCTGCCACATGAAGCTGAAGCAGCTATCAGCCGGTGCGACGGCTCTCCTGCTGCTTCTGCTGCTGCCGGCAGCTTTCTCCGATGCTGACACCCTCAGTACGTCTCAAAATGAGTTCGGCCCCTTCGACCAATCGTATTACAACAAGTTCGCGGTGCTCCTGCCGGCGACGATCAGCAACCAGGCGCTCCAGATCACGCCGGACTCCGCCGGAAACTTCACCCTCTACAACCGCTCAGGTAGAATCTTCTACAACCAATCTTTCACGCTCTGGAAAGATTTGGATTCAGATTCTCCCGATTCCGCAGCAAACGGAAGCCACGTGGCGTCTTTCAATTCGTCGTTCCTGATTAACGTCTTTCGTCTCAACGGCTCCGCCGTCCCAGGCGAGGGCCTGGCGTTCCTCATCGCGCCGAATATGACCCTCCCGGCGGCCAGCGACGGTCAATACCTCGGTTTGACCAACGCCACCACAGACGGAGACCCCAACAACCACTTCCTCGCCGTCGAGCTCGACACATTTAAACAAGATTTCGACCCGGACGACAACCACATGGGCATCAACATCAATTCAATCCGGTCCAACAAGACCGTTTCGCTCTCGGATGTGGATATCCAGATCGCGCCTCCCGGTACCCGATTTTACATGGTCTGGATCGAATACGGCGGCGAGTCGCACCAGTTAGACGTTTATATGGCCGAGCAAGACGGGGATCCGCAGACCGGGTTCGTTATCCGGCCCAGGCCCACAAGCCCAGTCCTAACCGCCAATCTCAACCTCAAGGACATAGTGAAACAGAAATCCTATTTCGGTTTCGCCGCGTCGACCGGGAGCGCGATCGAGCTCAATTGTGTGTTGAGATGGAATCTGACTGTGGAGCTTCTGCCAGGTGGTAAGGGTGGGAATAATAAGGGGTTGAAGATTGGGCTGGCGGTGGGTGTGCCTGCGGTGGCGCTGTTGCTGTTGGGCCTGGGCGGGTTGGGGTATTATTTGTACAAGCGGCGGAAGGCGGCGGCGTCGGACGAGAATATTTTGGGGGCGCTCAAGAGTCTTCCGGGCACTCCGAGGGAGTTTAGTTTCAAGGAATTGAAGAAGGCAACGAACAATTTCGACGATAAGCACAAACTGGGGCAAGGCGGATTTGGGGTGGTGTATAGAGGTGTGCTGCCCACAGAGAACATGGAGGTAGCGGTGAAGAAATTTTCCCGGGATAATCTCAAGGGCAAGGACGACTTTTTGGCTGAGCTCACCATTATCAACCGGCTCCGGCACAAGCATCTCGTCCGATTACTGGGATGGTGCCACAAAAATGGAATGCTACTCATAGTCTACGAGTTCATGCCCAACGGCAGCTTGGACAACCACCTGTTTGGCGAGACGGAAAATGCAACCCTAGGCTGGAGCCAGAGGTACAAAATCGTTGCCGGAGTAGCCTCCGCCCTACACTACCTCCACAATGAGTACGACCAACGAGTTGTCCATAGAGACCTAAAAGCCAGCAACATAATGCTAGACTCCAACTTCAACTCCCGCCTTGGGGACTTTGGCCTTGCGCGTGCCCTAGACAACGAGAAGACCTCGTACGCTGAGCTCGAGGGTGTTCCGGGCACGATGGGGTACATAGCCCCCGAGTGTTTCCACACGGGGAAAGCCACGTGCGAGTCCGATATTTACGGATTCGGGGCCGTGCTCCTAGAAGTCGTGTGTGGTCAACGGCCGTGGACCAAAATGGGAGGGTTCCAATTCATTGTGGATTGGGTTTGGTCGTTGCACAGAGAAGGGCGCGTGCTGGATGCCGTCGATGAGAGGCTGGGAAATGATTACGTGGCGGAGGAAGCCAAGAGGCTTTTGCTTCTTGGGCTGGCCTGCATGCACCCGATCGCGGGCGAGAGGCCCAAGACGCAAACTATTATCCAAATTATTTCCGGGTCGGTTCCCGTGCCCCAGGTCCCACCCTTTAAACCCGCTTTCGTGTGGCCCTCCAGCAATATGCCTGAGGGAGTGAGTAGCGAAGCAAACACAGTGAATACGACGTCGTCCTGGCCCCTTGAGATATCGGTGGCAGGTACTTCTGACTGGACCCCGCGCTACGACAGCCAGGATAGCAATGGAGGAACAAGACAACATAGTGACAGCTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lectin_legB Pkinase Pkinase_Tyr

Protein Analysis

727

Amino Acids

79.41

Weight (kDa)

5.82

Isoelectric Point (pI)

42.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lectin_legB PF00139 61 - 306 1.1e-57 Legume lectin domain
Pkinase PF00069 384 - 589 6.9e-47 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 386 - 595 3.8e-48 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000688)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g09820 FvH4_2g17160 FvH4_2g17160
malus_domestica MD02G1107100.v1.1 MD02G1107300.v1.1 MD05G1144900.v1.1 MD10G1144700.v1.1 MD15G1226500.v1.1
prunus_persica Prupe.7G188700_v2.0.a1 Prupe.7G188700_v2.0.a1 Prupe.7G188700_v2.0.a1 Prupe.8G183000_v2.0.a1
pyrus_communis pycom05g14000 pycom10g12440 pycom15g20140
rosa_chinensis RchiOBHm_Chr2g0096621 RchiOBHm_Chr6g0281601 RchiOBHm_Chr6g0281651 RchiOBHm_Chr7g0212121
rosa_laevigata RLG00000002926 RLG00000002927 RLG00000002933 RLG00000008638 RLG00000012954 RLG00000012955 RLG00000016656
rosa_multiflora Rmu_co8091766.1_g000001 Rmu_co8287847.1_g000001 Rmu_co8297221.1_g000001 Rmu_co8485327.1_g000001 Rmu_co8512767.1_g000001 Rmu_sc0002316.1_g000041 Rmu_sc0003565.1_g000009 Rmu_sc0014556.1_g000004 Rmu_sc0027573.1_g000001 Rmu_sc0030028.1_g000001
rosa_roxburghii Rroxscaffold_1G00026540 Rroxscaffold_2G00145470 Rroxscaffold_3G00247100 Rroxscaffold_3G00247110 Rroxscaffold_3G00247120 Rroxscaffold_5G00362680 Rroxscaffold_5G00362710 Rroxscaffold_7G00186950
rosa_rugosa Rorug02G0059000 Rorug02G0059100 Rorug06G0142300 Rorug07G0131000 Rorug07G0131100
rosa_samantha Rh1DG155700 Rh2AG106500 Rh2AG171500 Rh2BG108800 Rh2BG178900 Rh2CG110600 Rh2CG176400 Rh2DG110100 Rh2DG177600 Rh4BG120600 Rh6BG258300 Rh6CG257100 Rh6DG249200 Rh6DG249300 Rh7AG264100 Rh7AG264200 Rh7BG257800 Rh7BG257900 Rh7BG258000 Rh7BG258100 Rh7CG280600 Rh7CG280700 Rh7DG270600
rosa_wichuraiana Rw2G008240 Rw6G022110 Rw7G022540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 25
AatII GACGTC 2 cut(s) 23, 2075
Acc36I ACCTGC 1 cut(s) 2093
Acc65I GGTACC 1 cut(s) 671
AccB1I GGYRCC 2 cut(s) 671, 1338
AccBSI CCGCTC 1 cut(s) 273
AccI GTMKAC 2 cut(s) 863, 1368
AccII CGCG 9 cut(s) 191, 449, 662, 860, 875, 1594, 1824, 1922, 2125
AclWI GGATC 3 cut(s) 701, 746, 759
AcoI YGGCCR 3 cut(s) 471, 735, 1754
AcsI RAATTY 1 cut(s) 1238
AcuI CTGAAG 1 cut(s) 77
AcvI CACGTG 2 cut(s) 361, 1695
AcyI GRCGYC 4 cut(s) 20, 365, 1055, 2072
AfaI GTAC 8 cut(s) 139, 673, 1034, 1451, 1502, 1622, 1658, 2107
AgsI TTSAA 6 cut(s) 373, 612, 958, 1120, 1129, 1564
AjnI CCWGG 9 cut(s) 219, 421, 432, 779, 925, 1008, 1983, 2078, 2136
AjuI GAANNNNNNNTTGG 2 cut(s) 518, 550
Alw21I GWGCWC 6 cut(s) 198, 552, 885, 1287, 1632, 1731
Alw26I GTCTC 5 cut(s) 147, 407, 513, 1409, 1518
AlwI GGATC 3 cut(s) 701, 746, 759
Ama87I CYCGRG 3 cut(s) 1245, 1631, 1667
ApeKI GCWGC 9 cut(s) 42, 61, 91, 100, 103, 110, 344, 1204, 1389
ApoI RAATTY 1 cut(s) 1238
Asp700I GAANNNNTTC 1 cut(s) 1145
Asp718I GGTACC 1 cut(s) 671
AspA2I CCTAGG 1 cut(s) 1432
AspLEI GCGC 9 cut(s) 226, 451, 664, 875, 994, 1081, 1594, 1824, 2127
AsuHPI GGTGA 3 cut(s) 250, 1243, 1279
AvaI CYCGRG 3 cut(s) 1245, 1631, 1667
AvaII GGWCC 4 cut(s) 618, 1761, 1987, 2118
AvrII CCTAGG 1 cut(s) 1432
AxyI CCTNAGG 1 cut(s) 2034
BaeGI GKGCMC 4 cut(s) 1100, 1600, 1649, 1983
BamHI GGATCC 1 cut(s) 751
BanI GGYRCC 2 cut(s) 671, 1338
BanII GRGCYC 4 cut(s) 552, 885, 1287, 1632
BarI GAAGNNNNNNTAC 2 cut(s) 1604, 1636
BbrPI CACGTG 2 cut(s) 361, 1695
BbsI GAAGAC 3 cut(s) 21, 1082, 1619
Bbv12I GWGCWC 6 cut(s) 198, 552, 885, 1287, 1632, 1731
BbvI GCAGC 9 cut(s) 29, 73, 78, 87, 90, 122, 356, 1191, 1401
BccI CCATC 3 cut(s) 894, 1329, 1645
BcgI CGANNNNNNTGC 1 cut(s) 37
BciT130I CCWGG 9 cut(s) 221, 423, 434, 781, 927, 1010, 1985, 2080, 2138
BcoDI GTCTC 5 cut(s) 147, 407, 513, 1409, 1518
BfaI CTAG 5 cut(s) 1433, 1550, 1601, 1733, 2179
BfoI RGCGCY 3 cut(s) 227, 995, 1082
BfuAI ACCTGC 1 cut(s) 2093
BlnI CCTAGG 1 cut(s) 1432
BlpI GCTNAGC 2 cut(s) 1281, 1626
Bme18I GGWCC 4 cut(s) 618, 1761, 1987, 2118
BmeT110I CYCGRG 3 cut(s) 1245, 1631, 1667
BmrI ACTGGG 3 cut(s) 788, 1175, 1340
BmsI GCATC 4 cut(s) 112, 606, 1324, 1822
BmuI ACTGGG 3 cut(s) 788, 1175, 1340
BpiI GAAGAC 3 cut(s) 21, 1082, 1619
BplI GAGNNNNNCTC 2 cut(s) 1601, 1633
BpmI CTGGAG 3 cut(s) 212, 1459, 2005
Bpu10I CCTNAGC 1 cut(s) 274
Bpu1102I GCTNAGC 2 cut(s) 1281, 1626
BpuEI CTTGAG 4 cut(s) 803, 1067, 1241, 2108
BsaAI YACGTR 3 cut(s) 361, 1695, 1864
BsaHI GRCGYC 4 cut(s) 20, 365, 1055, 2072
BsaI GGTCTC 2 cut(s) 513, 1518
BsaWI WCCGGW 1 cut(s) 615
BsaXI ACNNNNNCTCC 4 cut(s) 1473, 1503, 1550, 1580
Bse118I RCCGGY 4 cut(s) 71, 106, 202, 1299
Bse1I ACTGG 5 cut(s) 718, 794, 1170, 1335, 2120
Bse21I CCTNAGG 1 cut(s) 2034
Bse3DI GCAATG 1 cut(s) 2152
BseBI CCWGG 9 cut(s) 221, 423, 434, 781, 927, 1010, 1985, 2080, 2138
BseGI GGATG 3 cut(s) 649, 1340, 1837
BseMI GCAATG 1 cut(s) 2152
BseMII CTCAG 5 cut(s) 148, 288, 1272, 1617, 2025
BseNI ACTGG 5 cut(s) 718, 794, 1170, 1335, 2120
BseSI GKGCMC 4 cut(s) 1100, 1600, 1649, 1983
BseX3I CGGCCG 1 cut(s) 1754
BseXI GCAGC 9 cut(s) 29, 73, 78, 87, 90, 122, 356, 1191, 1401
BseYI CCCAGC 1 cut(s) 1849
Bsh1236I CGCG 9 cut(s) 191, 449, 662, 860, 875, 1594, 1824, 1922, 2125
Bsh1285I CGRYCG 4 cut(s) 867, 879, 1757, 1920
BshNI GGYRCC 2 cut(s) 671, 1338
BsiEI CGRYCG 4 cut(s) 867, 879, 1757, 1920
BsiHKAI GWGCWC 6 cut(s) 198, 552, 885, 1287, 1632, 1731
BsiHKCI CYCGRG 3 cut(s) 1245, 1631, 1667
BsiWI CGTACG 1 cut(s) 1620
BslFI GGGAC 3 cut(s) 404, 1592, 1973
BsmAI GTCTC 5 cut(s) 147, 407, 513, 1409, 1518
BsmBI CGTCTC 3 cut(s) 147, 407, 1409
BsmFI GGGAC 3 cut(s) 404, 1592, 1973
BsmI GAATGC 1 cut(s) 1359
Bso31I GGTCTC 2 cut(s) 513, 1518
BsoBI CYCGRG 3 cut(s) 1245, 1631, 1667
Bsp1407I TGTACA 1 cut(s) 1032
Bsp143I GATC 7 cut(s) 210, 231, 657, 693, 751, 876, 1917
Bsp1720I GCTNAGC 2 cut(s) 1281, 1626
BspCNI CTCAG 5 cut(s) 147, 287, 1273, 1618, 2026
BspFNI CGCG 9 cut(s) 191, 449, 662, 860, 875, 1594, 1824, 1922, 2125
BspMI ACCTGC 1 cut(s) 2093
BspPI GGATC 3 cut(s) 701, 746, 759
BspT107I GGYRCC 2 cut(s) 671, 1338
BspTNI GGTCTC 2 cut(s) 513, 1518
BsrBI CCGCTC 1 cut(s) 273
BsrDI GCAATG 1 cut(s) 2152
BsrFI RCCGGY 4 cut(s) 71, 106, 202, 1299
BsrGI TGTACA 1 cut(s) 1032
BsrI ACTGG 5 cut(s) 718, 794, 1170, 1335, 2120
BssAI RCCGGY 4 cut(s) 71, 106, 202, 1299
BssMI GATC 7 cut(s) 210, 231, 657, 693, 751, 876, 1917
BssNI GRCGYC 4 cut(s) 20, 365, 1055, 2072
BssT1I CCWWGG 2 cut(s) 1432, 1573
Bst2UI CCWGG 9 cut(s) 221, 423, 434, 781, 927, 1010, 1985, 2080, 2138
Bst4CI ACNGT 4 cut(s) 482, 631, 913, 2062
BstACI GRCGYC 4 cut(s) 20, 365, 1055, 2072
BstAUI TGTACA 1 cut(s) 1032
BstBAI YACGTR 3 cut(s) 361, 1695, 1864
BstDEI CTNAG 5 cut(s) 134, 274, 1281, 1626, 2034
BstDSI CCRYGG 1 cut(s) 1757
BstF5I GGATG 3 cut(s) 649, 1340, 1837
BstFNI CGCG 9 cut(s) 191, 449, 662, 860, 875, 1594, 1824, 1922, 2125
BstH2I RGCGCY 3 cut(s) 227, 995, 1082
BstHHI GCGC 9 cut(s) 226, 451, 664, 875, 994, 1081, 1594, 1824, 2127
BstKTI GATC 7 cut(s) 213, 234, 660, 696, 754, 879, 1920
BstMAI GTCTC 5 cut(s) 147, 407, 513, 1409, 1518
BstMBI GATC 7 cut(s) 210, 231, 657, 693, 751, 876, 1917
BstMCI CGRYCG 4 cut(s) 867, 879, 1757, 1920
BstMWI GCNNNNNNNGC 9 cut(s) 61, 97, 100, 1469, 1874, 1897, 1939, 2133, 2142
BstNI CCWGG 9 cut(s) 221, 423, 434, 781, 927, 1010, 1985, 2080, 2138
BstNSI RCATGY 1 cut(s) 1912
BstSLI GKGCMC 4 cut(s) 1100, 1600, 1649, 1983
BstUI CGCG 9 cut(s) 191, 449, 662, 860, 875, 1594, 1824, 1922, 2125
BstV1I GCAGC 9 cut(s) 29, 73, 78, 87, 90, 122, 356, 1191, 1401
BstV2I GAAGAC 3 cut(s) 21, 1082, 1619
BstX2I RGATCY 1 cut(s) 751
BstXI CCANNNNNNTGG 2 cut(s) 1349, 1409
BstYI RGATCY 1 cut(s) 751
BstZI CGGCCG 1 cut(s) 1754
Bsu36I CCTNAGG 1 cut(s) 2034
BtgI CCRYGG 1 cut(s) 1757
BtgZI GCGATG 1 cut(s) 430
BtsCI GGATG 3 cut(s) 649, 1340, 1837
BtsIMutI CAGTG 1 cut(s) 2067
BveI ACCTGC 1 cut(s) 2093
CfoI GCGC 9 cut(s) 226, 451, 664, 875, 994, 1081, 1594, 1824, 2127
Cfr10I RCCGGY 4 cut(s) 71, 106, 202, 1299
Cfr9I CCCGGG 1 cut(s) 1245
CseI GACGC 4 cut(s) 354, 849, 1044, 1948
Csp6I GTAC 8 cut(s) 138, 672, 1033, 1450, 1501, 1621, 1657, 2106
CspCI CAANNNNNGTGG 2 cut(s) 1739, 1774
CviAII CATG 6 cut(s) 49, 592, 685, 1219, 1378, 1909
CviQI GTAC 8 cut(s) 138, 672, 1033, 1450, 1501, 1621, 1657, 2106
DdeI CTNAG 5 cut(s) 134, 274, 1281, 1626, 2034
DpnI GATC 7 cut(s) 212, 233, 659, 695, 753, 878, 1919
DpnII GATC 7 cut(s) 210, 231, 657, 693, 751, 876, 1917
DraI TTTAAA 2 cut(s) 562, 1999
DrdI GACNNNNNNGTC 1 cut(s) 25
DseDI GACNNNNNNGTC 1 cut(s) 25
EaeI YGGCCR 3 cut(s) 471, 735, 1754
EagI CGGCCG 1 cut(s) 1754
EciI GGCGGA 6 cut(s) 235, 403, 1057, 1191, 1465, 1883
Ecl136II GAGCTC 4 cut(s) 550, 883, 1285, 1630
EclXI CGGCCG 1 cut(s) 1754
Eco130I CCWWGG 2 cut(s) 1432, 1573
Eco24I GRGCYC 4 cut(s) 552, 885, 1287, 1632
Eco31I GGTCTC 2 cut(s) 513, 1518
Eco32I GATATC 2 cut(s) 652, 2094
Eco47I GGWCC 4 cut(s) 618, 1761, 1987, 2118
Eco52I CGGCCG 1 cut(s) 1754
Eco53kI GAGCTC 4 cut(s) 550, 883, 1285, 1630
Eco57I CTGAAG 1 cut(s) 77
Eco72I CACGTG 2 cut(s) 361, 1695
Eco81I CCTNAGG 1 cut(s) 2034
Eco88I CYCGRG 3 cut(s) 1245, 1631, 1667
EcoICRI GAGCTC 4 cut(s) 550, 883, 1285, 1630
EcoO109I RGGNCCY 2 cut(s) 430, 1987
EcoRII CCWGG 9 cut(s) 219, 421, 432, 779, 925, 1008, 1983, 2078, 2136
EcoRV GATATC 2 cut(s) 652, 2094
EcoT14I CCWWGG 2 cut(s) 1432, 1573
EcoT38I GRGCYC 4 cut(s) 552, 885, 1287, 1632
ErhI CCWWGG 2 cut(s) 1432, 1573
Esp3I CGTCTC 3 cut(s) 147, 407, 1409
FaeI CATG 6 cut(s) 52, 595, 688, 1222, 1381, 1912
FalI AAGNNNNNCTT 2 cut(s) 1256, 1288
FaqI GGGAC 3 cut(s) 404, 1592, 1973
FatI CATG 6 cut(s) 48, 591, 684, 1218, 1377, 1908
FauI CCCGC 5 cut(s) 1007, 1577, 1915, 2011, 2130
FblI GTMKAC 2 cut(s) 863, 1368
FokI GGATG 3 cut(s) 656, 1347, 1844
FriOI GRGCYC 4 cut(s) 552, 885, 1287, 1632
FspBI CTAG 5 cut(s) 1433, 1550, 1601, 1733, 2179
GlaI GCGC 9 cut(s) 225, 450, 663, 874, 993, 1080, 1593, 1823, 2126
GsaI CCCAGC 1 cut(s) 1853
GsuI CTGGAG 3 cut(s) 212, 1459, 2005
HaeII RGCGCY 3 cut(s) 227, 995, 1082
HgaI GACGC 4 cut(s) 354, 849, 1044, 1948
HhaI GCGC 9 cut(s) 226, 451, 664, 875, 994, 1081, 1594, 1824, 2127
Hin1I GRCGYC 4 cut(s) 20, 365, 1055, 2072
Hin1II CATG 6 cut(s) 52, 595, 688, 1222, 1381, 1912
Hin6I GCGC 9 cut(s) 224, 449, 662, 873, 992, 1079, 1592, 1822, 2125
HinP1I GCGC 9 cut(s) 224, 449, 662, 873, 992, 1079, 1592, 1822, 2125
HincII GTYRAC 2 cut(s) 864, 1751
HindII GTYRAC 2 cut(s) 864, 1751
HphI GGTGA 3 cut(s) 250, 1243, 1279
Hpy166II GTNNAC 4 cut(s) 864, 1369, 1751, 1761
Hpy188I TCNGA 9 cut(s) 121, 328, 643, 909, 1060, 1104, 1325, 1705, 2113
Hpy188III TCNNGA 7 cut(s) 229, 310, 335, 385, 655, 691, 1084
Hpy8I GTNNAC 4 cut(s) 864, 1369, 1751, 1761
HpyAV CCTTC 4 cut(s) 172, 1039, 1126, 1811
HpyCH4III ACNGT 4 cut(s) 482, 631, 913, 2062
HpyCH4IV ACGT 8 cut(s) 20, 140, 360, 393, 726, 1694, 1863, 2072
HpyCH4V TGCA 5 cut(s) 14, 1427, 1810, 1908, 1912
HpyF10VI GCNNNNNNNGC 9 cut(s) 61, 97, 100, 1469, 1874, 1897, 1939, 2133, 2142
HpyF3I CTNAG 5 cut(s) 134, 274, 1281, 1626, 2034
HpySE526I ACGT 8 cut(s) 20, 140, 360, 393, 726, 1694, 1863, 2072
Hsp92I GRCGYC 4 cut(s) 20, 365, 1055, 2072
Hsp92II CATG 6 cut(s) 52, 595, 688, 1222, 1381, 1912
HspAI GCGC 9 cut(s) 224, 449, 662, 873, 992, 1079, 1592, 1822, 2125
KpnI GGTACC 1 cut(s) 675
KroI GCCGGC 2 cut(s) 106, 202
KroNI GCCGGC 2 cut(s) 108, 204
Kzo9I GATC 7 cut(s) 210, 231, 657, 693, 751, 876, 1917
LmnI GCTCC 8 cut(s) 201, 231, 416, 870, 916, 1308, 1440, 1734
Lsp1109I GCAGC 9 cut(s) 29, 73, 78, 87, 90, 122, 356, 1191, 1401
LweI GCATC 4 cut(s) 112, 606, 1324, 1822
MaeI CTAG 5 cut(s) 1433, 1550, 1601, 1733, 2179
MaeII ACGT 8 cut(s) 20, 140, 360, 393, 726, 1694, 1863, 2072
MaeIII GTNAC 1 cut(s) 2168
MalI GATC 7 cut(s) 212, 233, 659, 695, 753, 878, 1919
MbiI CCGCTC 1 cut(s) 273
MboI GATC 7 cut(s) 210, 231, 657, 693, 751, 876, 1917
MboII GAAGA 9 cut(s) 21, 24, 27, 277, 970, 1082, 1141, 1246, 1624
MfeI CAATTG 1 cut(s) 886
MflI RGATCY 1 cut(s) 751
MluCI AATT 8 cut(s) 373, 607, 886, 1124, 1144, 1238, 1781, 1956
MlyI GAGTC 5 cut(s) 236, 719, 1096, 1547, 1709
MmeI TCCRAC 3 cut(s) 645, 1038, 1581
MroNI GCCGGC 2 cut(s) 106, 202
MroXI GAANNNNTTC 1 cut(s) 1145
MseI TTAA 3 cut(s) 390, 561, 1998
MslI CAYNNNNRTG 1 cut(s) 1347
MspA1I CMGCKG 1 cut(s) 42
MunI CAATTG 1 cut(s) 886
Mva1269I GAATGC 1 cut(s) 1359
MvaI CCWGG 9 cut(s) 221, 423, 434, 781, 927, 1010, 1985, 2080, 2138
MvnI CGCG 9 cut(s) 191, 449, 662, 860, 875, 1594, 1824, 1922, 2125
MwoI GCNNNNNNNGC 9 cut(s) 61, 97, 100, 1469, 1874, 1897, 1939, 2133, 2142
NaeI GCCGGC 2 cut(s) 108, 204
NdeII GATC 7 cut(s) 210, 231, 657, 693, 751, 876, 1917
NgoMIV GCCGGC 2 cut(s) 106, 202
NlaIII CATG 6 cut(s) 52, 595, 688, 1222, 1381, 1912
NmeAIII GCCGAG 1 cut(s) 763
NmuCI GTSAC 1 cut(s) 2168
NspI RCATGY 1 cut(s) 1912
PaeI GCATGC 1 cut(s) 1912
PaeR7I CTCGAG 1 cut(s) 1631
PcsI WCGNNNNNNNCGW 6 cut(s) 23, 162, 357, 414, 579, 1629
PctI GAATGC 1 cut(s) 1359
PdiI GCCGGC 2 cut(s) 108, 204
PdmI GAANNNNTTC 1 cut(s) 1145
PfeI GAWTC 6 cut(s) 282, 323, 329, 338, 904, 1716
Pfl23II CGTACG 1 cut(s) 1620
PflFI GACNNNGTC 2 cut(s) 22, 2074
Ple19I CGATCG 2 cut(s) 879, 1920
PleI GAGTC 5 cut(s) 236, 718, 1095, 1547, 1708
PmaCI CACGTG 2 cut(s) 361, 1695
PmlI CACGTG 2 cut(s) 361, 1695
PpsI GAGTC 5 cut(s) 236, 718, 1095, 1547, 1708
Ppu21I YACGTR 3 cut(s) 361, 1695, 1864
PpuMI RGGWCCY 1 cut(s) 1987
Psp124BI GAGCTC 4 cut(s) 552, 885, 1287, 1632
Psp5II RGGWCCY 1 cut(s) 1987
Psp6I CCWGG 9 cut(s) 219, 421, 432, 779, 925, 1008, 1983, 2078, 2136
PspCI CACGTG 2 cut(s) 361, 1695
PspFI CCCAGC 1 cut(s) 1849
PspGI CCWGG 9 cut(s) 219, 421, 432, 779, 925, 1008, 1983, 2078, 2136
PspLI CGTACG 1 cut(s) 1620
PspPPI RGGWCCY 1 cut(s) 1987
PspXI VCTCGAGB 1 cut(s) 1631
PsuI RGATCY 1 cut(s) 751
PsyI GACNNNGTC 2 cut(s) 22, 2074
PvuI CGATCG 2 cut(s) 879, 1920
PvuII CAGCTG 1 cut(s) 42
RsaI GTAC 8 cut(s) 139, 673, 1034, 1451, 1502, 1622, 1658, 2107
RsaNI GTAC 8 cut(s) 138, 672, 1033, 1450, 1501, 1621, 1657, 2106
RseI CAYNNNNRTG 1 cut(s) 1347
SacI GAGCTC 4 cut(s) 552, 885, 1287, 1632
SalI GTCGAC 1 cut(s) 862
SaqAI TTAA 3 cut(s) 390, 561, 1998
Sau3AI GATC 7 cut(s) 210, 231, 657, 693, 751, 876, 1917
SchI GAGTC 5 cut(s) 236, 719, 1096, 1547, 1709
SfaNI GCATC 4 cut(s) 112, 606, 1324, 1822
Sfr274I CTCGAG 1 cut(s) 1631
SinI GGWCC 4 cut(s) 618, 1761, 1987, 2118
SlaI CTCGAG 1 cut(s) 1631
SmaI CCCGGG 1 cut(s) 1247
SmiMI CAYNNNNRTG 1 cut(s) 1347
SmlI CTYRAG 5 cut(s) 818, 1082, 1256, 1631, 2087
SmoI CTYRAG 5 cut(s) 818, 1082, 1256, 1631, 2087
SphI GCATGC 1 cut(s) 1912
Sse9I AATT 8 cut(s) 373, 607, 886, 1124, 1144, 1238, 1781, 1956
SspI AATATT 1 cut(s) 1069
SspMI CTAG 5 cut(s) 1433, 1550, 1601, 1733, 2179
SstI GAGCTC 4 cut(s) 552, 885, 1287, 1632
StyI CCWWGG 2 cut(s) 1432, 1573
TaaI ACNGT 4 cut(s) 482, 631, 913, 2062
TaiI ACGT 8 cut(s) 23, 143, 363, 396, 729, 1697, 1866, 2075
TasI AATT 8 cut(s) 373, 607, 886, 1124, 1144, 1238, 1781, 1956
TatI WGTACW 1 cut(s) 1032
TauI GCSGC 6 cut(s) 473, 708, 860, 1042, 1052, 1055
TfiI GAWTC 6 cut(s) 282, 323, 329, 338, 904, 1716
Tru1I TTAA 3 cut(s) 390, 561, 1998
Tru9I TTAA 3 cut(s) 390, 561, 1998
TscAI CASTG 1 cut(s) 2067
TseFI GTSAC 1 cut(s) 2168
TseI GCWGC 9 cut(s) 42, 61, 91, 100, 103, 110, 344, 1204, 1389
Tsp45I GTSAC 1 cut(s) 2168
TspDTI ATGAA 3 cut(s) 65, 1366, 1774
TspGWI ACGGA 4 cut(s) 366, 531, 1433, 1728
TspMI CCCGGG 1 cut(s) 1245
TspRI CASTG 1 cut(s) 2067
Tth111I GACNNNGTC 2 cut(s) 22, 2074
VpaK11BI GGWCC 4 cut(s) 618, 1761, 1987, 2118
XapI RAATTY 1 cut(s) 1238
XceI RCATGY 1 cut(s) 1912
XcmI CCANNNNNNNNNTGG 3 cut(s) 1216, 1390, 2144
XhoI CTCGAG 1 cut(s) 1631
XmaI CCCGGG 1 cut(s) 1245
XmaJI CCTAGG 1 cut(s) 1432
XmiI GTMKAC 2 cut(s) 863, 1368
XmnI GAANNNNTTC 1 cut(s) 1145
XspI CTAG 5 cut(s) 1433, 1550, 1601, 1733, 2179
ZraI GACGTC 2 cut(s) 21, 2073
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.