Belongs to the protein kinase superfamily
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 2 cut(s) | 234, 859 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 191 |
| AccBSI | CCGCTC | 1 cut(s) | 62 |
| AccI | GTMKAC | 1 cut(s) | 913 |
| AccII | CGCG | 1 cut(s) | 58 |
| AciI | CCGC | 2 cut(s) | 62, 757 |
| AclWI | GGATC | 2 cut(s) | 631, 702 |
| AcuI | CTGAAG | 2 cut(s) | 465, 824 |
| AfaI | GTAC | 1 cut(s) | 858 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 191, 620 |
| AflII | CTTAAG | 1 cut(s) | 124 |
| AgsI | TTSAA | 8 cut(s) | 85, 166, 209, 224, 313, 382, 455, 853 |
| AjnI | CCWGG | 1 cut(s) | 690 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 98, 130 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 165, 197 |
| AluBI | AGCT | 4 cut(s) | 301, 479, 751, 899 |
| AluI | AGCT | 4 cut(s) | 301, 479, 751, 899 |
| Alw21I | GWGCWC | 1 cut(s) | 767 |
| Alw26I | GTCTC | 4 cut(s) | 98, 185, 477, 884 |
| AlwI | GGATC | 2 cut(s) | 631, 702 |
| AoxI | GGCC | 1 cut(s) | 135 |
| ApeKI | GCWGC | 5 cut(s) | 26, 276, 298, 352, 551 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 883, 915 |
| AspLEI | GCGC | 2 cut(s) | 785, 868 |
| AspS9I | GGNCC | 2 cut(s) | 688, 977 |
| AsuHPI | GGTGA | 1 cut(s) | 934 |
| AvaII | GGWCC | 2 cut(s) | 688, 977 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 146, 179 |
| BbsI | GAAGAC | 2 cut(s) | 672, 830 |
| Bbv12I | GWGCWC | 1 cut(s) | 767 |
| BbvI | GCAGC | 5 cut(s) | 38, 263, 310, 364, 563 |
| BccI | CCATC | 1 cut(s) | 185 |
| BciT130I | CCWGG | 1 cut(s) | 692 |
| BciVI | GTATCC | 1 cut(s) | 147 |
| BclI | TGATCA | 1 cut(s) | 924 |
| BcoDI | GTCTC | 4 cut(s) | 98, 185, 477, 884 |
| BfaI | CTAG | 2 cut(s) | 713, 752 |
| BfmI | CTRYAG | 1 cut(s) | 492 |
| BfrI | CTTAAG | 1 cut(s) | 124 |
| BfuAI | ACCTGC | 2 cut(s) | 234, 859 |
| BfuI | GTATCC | 1 cut(s) | 147 |
| BglII | AGATCT | 1 cut(s) | 375 |
| BisI | GCNGC | 5 cut(s) | 27, 277, 299, 353, 552 |
| BlpI | GCTNAGC | 1 cut(s) | 294 |
| BlsI | GCNGC | 5 cut(s) | 28, 278, 300, 354, 553 |
| Bme1390I | CCNGG | 1 cut(s) | 692 |
| Bme18I | GGWCC | 2 cut(s) | 688, 977 |
| BmgT120I | GGNCC | 2 cut(s) | 688, 977 |
| BmiI | GGNNCC | 1 cut(s) | 689 |
| BmrFI | CCNGG | 1 cut(s) | 692 |
| BmsI | GCATC | 2 cut(s) | 536, 820 |
| BpiI | GAAGAC | 2 cut(s) | 672, 830 |
| BpmI | CTGGAG | 1 cut(s) | 459 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 294 |
| BpuEI | CTTGAG | 1 cut(s) | 842 |
| BsaI | GGTCTC | 1 cut(s) | 884 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 165, 195 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 191, 620 |
| Bse1I | ACTGG | 3 cut(s) | 12, 636, 701 |
| BseBI | CCWGG | 1 cut(s) | 692 |
| BseGI | GGATG | 2 cut(s) | 835, 1001 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 191, 620 |
| BseMII | CTCAG | 3 cut(s) | 167, 285, 851 |
| BseNI | ACTGG | 3 cut(s) | 12, 636, 701 |
| BseRI | GAGGAG | 1 cut(s) | 953 |
| BseXI | GCAGC | 5 cut(s) | 38, 263, 310, 364, 563 |
| BsgI | GTGCAG | 2 cut(s) | 262, 765 |
| Bsh1236I | CGCG | 1 cut(s) | 58 |
| BshFI | GGCC | 1 cut(s) | 137 |
| BsiHKAI | GWGCWC | 1 cut(s) | 767 |
| BsiSI | CCGG | 1 cut(s) | 138 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 191, 620 |
| BsmAI | GTCTC | 4 cut(s) | 98, 185, 477, 884 |
| BsmI | GAATGC | 1 cut(s) | 732 |
| BsnI | GGCC | 1 cut(s) | 137 |
| Bso31I | GGTCTC | 1 cut(s) | 884 |
| Bsp1286I | GDGCHC | 1 cut(s) | 767 |
| Bsp143I | GATC | 6 cut(s) | 202, 375, 623, 660, 694, 924 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 294 |
| Bsp68I | TCGCGA | 1 cut(s) | 58 |
| BspACI | CCGC | 2 cut(s) | 62, 757 |
| BspANI | GGCC | 1 cut(s) | 137 |
| BspCNI | CTCAG | 3 cut(s) | 168, 286, 852 |
| BspFNI | CGCG | 1 cut(s) | 58 |
| BspHI | TCATGA | 1 cut(s) | 951 |
| BspLI | GGNNCC | 1 cut(s) | 689 |
| BspMI | ACCTGC | 2 cut(s) | 234, 859 |
| BspPI | GGATC | 2 cut(s) | 631, 702 |
| BspTI | CTTAAG | 1 cut(s) | 124 |
| BspTNI | GGTCTC | 1 cut(s) | 884 |
| BsrBI | CCGCTC | 1 cut(s) | 62 |
| BsrI | ACTGG | 3 cut(s) | 12, 636, 701 |
| BssMI | GATC | 6 cut(s) | 202, 375, 623, 660, 694, 924 |
| BssNAI | GTATAC | 1 cut(s) | 914 |
| Bst1107I | GTATAC | 1 cut(s) | 914 |
| Bst2UI | CCWGG | 1 cut(s) | 692 |
| Bst4CI | ACNGT | 2 cut(s) | 50, 92 |
| BstAFI | CTTAAG | 1 cut(s) | 124 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 560 |
| BstC8I | GCNNGC | 3 cut(s) | 60, 357, 877 |
| BstDEI | CTNAG | 3 cut(s) | 176, 294, 860 |
| BstF5I | GGATG | 2 cut(s) | 835, 1001 |
| BstFNI | CGCG | 1 cut(s) | 58 |
| BstHHI | GCGC | 2 cut(s) | 785, 868 |
| BstKTI | GATC | 6 cut(s) | 205, 378, 626, 663, 697, 927 |
| BstMAI | GTCTC | 4 cut(s) | 98, 185, 477, 884 |
| BstMBI | GATC | 6 cut(s) | 202, 375, 623, 660, 694, 924 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 26, 35, 560, 872 |
| BstNI | CCWGG | 1 cut(s) | 692 |
| BstNSI | RCATGY | 1 cut(s) | 837 |
| BstSCI | CCNGG | 1 cut(s) | 690 |
| BstSFI | CTRYAG | 1 cut(s) | 492 |
| BstUI | CGCG | 1 cut(s) | 58 |
| BstV1I | GCAGC | 5 cut(s) | 38, 263, 310, 364, 563 |
| BstV2I | GAAGAC | 2 cut(s) | 672, 830 |
| BstX2I | RGATCY | 2 cut(s) | 375, 623 |
| BstYI | RGATCY | 2 cut(s) | 375, 623 |
| BstZ17I | GTATAC | 1 cut(s) | 914 |
| BsuI | GTATCC | 1 cut(s) | 147 |
| BsuRI | GGCC | 1 cut(s) | 137 |
| BtgZI | GCGATG | 1 cut(s) | 894 |
| BtsCI | GGATG | 2 cut(s) | 835, 1001 |
| BtsIMutI | CAGTG | 1 cut(s) | 5 |
| BtuMI | TCGCGA | 1 cut(s) | 58 |
| BveI | ACCTGC | 2 cut(s) | 234, 859 |
| Cac8I | GCNNGC | 3 cut(s) | 60, 357, 877 |
| CciI | TCATGA | 1 cut(s) | 951 |
| CfoI | GCGC | 2 cut(s) | 785, 868 |
| Cfr13I | GGNCC | 2 cut(s) | 688, 977 |
| Csp6I | GTAC | 1 cut(s) | 857 |
| CviAII | CATG | 3 cut(s) | 670, 834, 952 |
| CviJI | RGCY | 7 cut(s) | 137, 301, 479, 655, 751, 899, 998 |
| CviKI_1 | RGCY | 7 cut(s) | 137, 301, 479, 655, 751, 899, 998 |
| CviQI | GTAC | 1 cut(s) | 857 |
| DdeI | CTNAG | 3 cut(s) | 176, 294, 860 |
| DpnI | GATC | 6 cut(s) | 204, 377, 625, 662, 696, 926 |
| DpnII | GATC | 6 cut(s) | 202, 375, 623, 660, 694, 924 |
| Eco31I | GGTCTC | 1 cut(s) | 884 |
| Eco47I | GGWCC | 2 cut(s) | 688, 977 |
| Eco57I | CTGAAG | 2 cut(s) | 465, 824 |
| EcoO109I | RGGNCCY | 1 cut(s) | 688 |
| EcoRII | CCWGG | 1 cut(s) | 690 |
| EcoT22I | ATGCAT | 2 cut(s) | 732, 835 |
| FaeI | CATG | 3 cut(s) | 673, 837, 955 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 362, 394 |
| FatI | CATG | 3 cut(s) | 669, 833, 951 |
| FauNDI | CATATG | 1 cut(s) | 732 |
| FbaI | TGATCA | 1 cut(s) | 924 |
| FblI | GTMKAC | 1 cut(s) | 913 |
| Fnu4HI | GCNGC | 5 cut(s) | 27, 277, 299, 353, 552 |
| FokI | GGATG | 2 cut(s) | 842, 988 |
| Fsp4HI | GCNGC | 5 cut(s) | 27, 277, 299, 353, 552 |
| FspBI | CTAG | 2 cut(s) | 713, 752 |
| GlaI | GCGC | 2 cut(s) | 784, 867 |
| GluI | GCNGC | 5 cut(s) | 27, 277, 299, 353, 552 |
| GsuI | CTGGAG | 1 cut(s) | 459 |
| HaeIII | GGCC | 1 cut(s) | 137 |
| HapII | CCGG | 1 cut(s) | 138 |
| HhaI | GCGC | 2 cut(s) | 785, 868 |
| Hin1II | CATG | 3 cut(s) | 673, 837, 955 |
| Hin6I | GCGC | 2 cut(s) | 783, 866 |
| HinP1I | GCGC | 2 cut(s) | 783, 866 |
| HinfI | GANTC | 7 cut(s) | 119, 141, 178, 330, 461, 470, 958 |
| HpaII | CCGG | 1 cut(s) | 138 |
| HphI | GGTGA | 1 cut(s) | 934 |
| Hpy166II | GTNNAC | 2 cut(s) | 67, 914 |
| Hpy188I | TCNGA | 3 cut(s) | 484, 725, 843 |
| Hpy188III | TCNNGA | 7 cut(s) | 57, 206, 323, 379, 641, 821, 952 |
| Hpy8I | GTNNAC | 2 cut(s) | 67, 914 |
| HpyAV | CCTTC | 8 cut(s) | 67, 218, 364, 449, 497, 530, 583, 913 |
| HpyCH4III | ACNGT | 2 cut(s) | 50, 92 |
| HpyCH4V | TGCA | 7 cut(s) | 243, 279, 352, 730, 746, 833, 875 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 26, 35, 560, 872 |
| HpyF3I | CTNAG | 3 cut(s) | 176, 294, 860 |
| Hsp92II | CATG | 3 cut(s) | 673, 837, 955 |
| HspAI | GCGC | 2 cut(s) | 783, 866 |
| Ksp22I | TGATCA | 1 cut(s) | 924 |
| Kzo9I | GATC | 6 cut(s) | 202, 375, 623, 660, 694, 924 |
| LmnI | GCTCC | 2 cut(s) | 23, 770 |
| Lsp1109I | GCAGC | 5 cut(s) | 38, 263, 310, 364, 563 |
| LweI | GCATC | 2 cut(s) | 536, 820 |
| MaeI | CTAG | 2 cut(s) | 713, 752 |
| MalI | GATC | 6 cut(s) | 204, 377, 625, 662, 696, 926 |
| MbiI | CCGCTC | 1 cut(s) | 62 |
| MboI | GATC | 6 cut(s) | 202, 375, 623, 660, 694, 924 |
| MboII | GAAGA | 6 cut(s) | 445, 448, 677, 811, 830, 967 |
| MflI | RGATCY | 2 cut(s) | 375, 623 |
| MhlI | GDGCHC | 1 cut(s) | 767 |
| MluCI | AATT | 2 cut(s) | 308, 933 |
| MlyI | GAGTC | 4 cut(s) | 128, 187, 324, 479 |
| MmeI | TCCRAC | 1 cut(s) | 807 |
| MnlI | CCTC | 8 cut(s) | 122, 143, 163, 508, 837, 879, 931, 988 |
| Mph1103I | ATGCAT | 2 cut(s) | 732, 835 |
| MseI | TTAA | 3 cut(s) | 125, 650, 705 |
| MspCI | CTTAAG | 1 cut(s) | 124 |
| MspI | CCGG | 1 cut(s) | 138 |
| MspR9I | CCNGG | 1 cut(s) | 692 |
| Mva1269I | GAATGC | 1 cut(s) | 732 |
| MvaI | CCWGG | 1 cut(s) | 692 |
| MvnI | CGCG | 1 cut(s) | 58 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 26, 35, 560, 872 |
| NdeI | CATATG | 1 cut(s) | 732 |
| NdeII | GATC | 6 cut(s) | 202, 375, 623, 660, 694, 924 |
| NlaIII | CATG | 3 cut(s) | 673, 837, 955 |
| NlaIV | GGNNCC | 1 cut(s) | 689 |
| NruI | TCGCGA | 1 cut(s) | 58 |
| NsiI | ATGCAT | 2 cut(s) | 732, 835 |
| NspI | RCATGY | 1 cut(s) | 837 |
| PagI | TCATGA | 1 cut(s) | 951 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 154 |
| PctI | GAATGC | 1 cut(s) | 732 |
| PfeI | GAWTC | 3 cut(s) | 141, 461, 958 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 191 |
| PfoI | TCCNGGA | 1 cut(s) | 690 |
| PkrI | GCNGC | 5 cut(s) | 28, 278, 300, 354, 553 |
| PleI | GAGTC | 4 cut(s) | 127, 186, 324, 478 |
| PpsI | GAGTC | 4 cut(s) | 127, 186, 324, 478 |
| PpuMI | RGGWCCY | 1 cut(s) | 688 |
| Psp5II | RGGWCCY | 1 cut(s) | 688 |
| Psp6I | CCWGG | 1 cut(s) | 690 |
| PspGI | CCWGG | 1 cut(s) | 690 |
| PspN4I | GGNNCC | 1 cut(s) | 689 |
| PspPI | GGNCC | 2 cut(s) | 688, 977 |
| PspPPI | RGGWCCY | 1 cut(s) | 688 |
| PsuI | RGATCY | 2 cut(s) | 375, 623 |
| RruI | TCGCGA | 1 cut(s) | 58 |
| RsaI | GTAC | 1 cut(s) | 858 |
| RsaNI | GTAC | 1 cut(s) | 857 |
| SaqAI | TTAA | 3 cut(s) | 125, 650, 705 |
| SatI | GCNGC | 5 cut(s) | 27, 277, 299, 353, 552 |
| Sau3AI | GATC | 6 cut(s) | 202, 375, 623, 660, 694, 924 |
| Sau96I | GGNCC | 2 cut(s) | 688, 977 |
| SchI | GAGTC | 4 cut(s) | 128, 187, 324, 479 |
| ScrFI | CCNGG | 1 cut(s) | 692 |
| SduI | GDGCHC | 1 cut(s) | 767 |
| SfaNI | GCATC | 2 cut(s) | 536, 820 |
| SfcI | CTRYAG | 1 cut(s) | 492 |
| SinI | GGWCC | 2 cut(s) | 688, 977 |
| SmlI | CTYRAG | 2 cut(s) | 124, 821 |
| SmoI | CTYRAG | 2 cut(s) | 124, 821 |
| Sse9I | AATT | 2 cut(s) | 308, 933 |
| SsiI | CCGC | 2 cut(s) | 62, 757 |
| SspMI | CTAG | 2 cut(s) | 713, 752 |
| StyD4I | CCNGG | 1 cut(s) | 690 |
| TaaI | ACNGT | 2 cut(s) | 50, 92 |
| TaqI | TCGA | 3 cut(s) | 148, 663, 902 |
| TasI | AATT | 2 cut(s) | 308, 933 |
| TatI | WGTACW | 1 cut(s) | 856 |
| TfiI | GAWTC | 3 cut(s) | 141, 461, 958 |
| Tru1I | TTAA | 3 cut(s) | 125, 650, 705 |
| Tru9I | TTAA | 3 cut(s) | 125, 650, 705 |
| TscAI | CASTG | 1 cut(s) | 12 |
| TseI | GCWGC | 5 cut(s) | 26, 276, 298, 352, 551 |
| TspDTI | ATGAA | 8 cut(s) | 119, 321, 429, 446, 516, 968, 986, 990 |
| TspGWI | ACGGA | 1 cut(s) | 969 |
| TspRI | CASTG | 1 cut(s) | 12 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 191 |
| Vha464I | CTTAAG | 1 cut(s) | 124 |
| VpaK11BI | GGWCC | 2 cut(s) | 688, 977 |
| XceI | RCATGY | 1 cut(s) | 837 |
| XmiI | GTMKAC | 1 cut(s) | 913 |
| XspI | CTAG | 2 cut(s) | 713, 752 |
| Zsp2I | ATGCAT | 2 cut(s) | 732, 835 |