RchiOBHm_Chr2g0096621

L-type lectin-domain containing receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0000688
Physical Location & Seq
Reverse (-)
9363824 .. 9367181
3358 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ47153

Sequence Viewer

Length: 2121 bp
ATGGTGATTCTGTTTCTCTCCCTTCTGTTGTTGCCGGAGGCGGCTTTCACCGACGCTCAGACCTACGAGGTGAAACCCTACCAGTTCGGTCCCTTTGATCCAACATATTACGACAGGTTCGCAGTCCTCAGCCCCGCCACGATCAACTTAGGAGCTCTTCAGATCACGCCGGACTCCGCCAATGGCAACTTCAGCAAGGCCAACAAGTCTGGTAGGATCTTGTACAACCAGTCTTTCACCCTCTGGAACTCTGATCTCGACTCTTCTTCTCCGGGATACGTCGCCTCTTTCAACTCGTCCTTCCTCATTAACGTCTATCGTCTCAACAGCTCCGGCATCCACGAGGACTCCGCCGTCCCCGGAGAGGGCATCGCCTTCATTATAGCCCCGGATTTGACCCTCCCAGCAAACAGCGCCGGTCAATATCTCGGTTTGACCAACGCCACCACCGACGGTAACCCCACCAATCACTTGGTGGCAATTGAGCTCGACACTTTGAAGGAGGGCTTCGATCCGGACAGCAACCACATGGGGCTGAATATCAACTCGGTTAAGTCTAACAAGACGGTGCCGCTTTGGGATTACGGGATCGAGATCGCGCCACCAGGCACGGAGTTTTATATGGTCTGGATCCAATACGACGGTGGGTCACATAATCTGTCGGTGTTCATGGCCAACCAGACCGATCAGAGCAAGGACGGGAAAGGAGCGTTTGTTAATATCCGGCCTCGGCCCACCAGCCCAATTCTAACCGCCAACCTTAACCTCAAGGACATTGTCAACCAAAAGTCCTACTTCGGATTTGCCGCGTCCACCGGAAGTAACATCCAGCTCAACTGCGTGTTGATGTGGAATCTAACGGTGGAGATTCTGCCGGACTTGGAAGGTGGAGGGGACAACGATAAGGTGTTGAAGATAGCACTAGGGGTGGGTGCCGGGTTGGTTTTAGTCCTACTGTGTTTATTCGGGGTGGGTTGTTATGTGTACAAAAGGCGAAAGGCTAAGGCGTCGGACCCTAACATTTTGGGCGCGCTTAAGAGCCTTCCCGGAACACCAAGGGAGTTCAGTTACCGAGAGCTTAAGAAAGCCACCAACAACTTCGACGACAAGCACAAATTAGGCCAAGGTGGGTTCGGGGTGGTATACCGAGCTGTGCTAGCTAAGGAGGATAACCTCGAAGTGGCAGTGAAGATGTTCTCAAGGGACGAGGTTAAGGGCAGAGACGACTTTTTGGCCGAGCTCACCATCATTAACCGCCTTCGCCACAAACACCTCGTCCGGTTACTCGGTTGGTGCCACAAGAATGGGATGCTTCTCATTGTCTACGAGTACATGCCTAACGGGAGCTTGGACAACCACCTATTCTCCGGGCCGGAAAACACAACCCTAGGATGGAAACAGAGGTACAAGATCGTCACGGGTGTGGCCTCAGCCCTACACTACCTCCACAACGAGTACGACCAAAGAGTAGTCCACAGAGACCTCAAGGCTAGCAACATAATGCTTGACACCCACTTCAACGCGCGCCTAGGCGACTTCGGCTTGGCGCGCGCTCTGGACAATGAAAAGACCTCATACGCTGAACTCGAAGGTGTCCCGGGCACGATGGGGTACATCGCCCCGGAGTGCTTCCACACGGGAAAAGCCACATGCGAGTCTGACGTGTACGGGTTCGGGGCGGTGGTTCTAGAAGTTGTGTGCGGTCAACGGCCGTGGACTAAAATCGGCGGATACCAATTTCTAGTGGATTGGGTATGGTCGTTGCATAGAGAAGGGCGTGTGCTGGAGGCTGTGGACGAGAGGTTGGGTAATGACTACGTAATTGAGGAGGCCCAAAGGCTTTTGCTTCTTGGGCTGGCTTGTTCCCATCCAATTGCGAACGAAAGGCCCAAGACCCAAACTATAGTTCAAATCATTTCCGGGTCGGTTCCAGTCCCCCGAGTCCCACCTTTCAAACCCTCTTTCGTGTGGCCTTCCATGCCGGAAGGAGTGAGCAGCATTGCCAACACGGTTAACACGACGTCGTCCTGGCCTGTCGGGATGTCAGCCTCCGATAGGTCTGAGTGGAGCCCACATTTGGACAGCAGGGATATATATAGTGACAGCTCTTCCCTGGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lectin_legB Pkinase Pkinase_Tyr

Protein Analysis

706

Amino Acids

77.78

Weight (kDa)

5.82

Isoelectric Point (pI)

38.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lectin_legB PF00139 37 - 289 5.5e-55 Legume lectin domain
Pkinase PF00069 368 - 576 2.5e-47 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 370 - 580 5e-49 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000688)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g09820 FvH4_2g17160 FvH4_2g17160
malus_domestica MD02G1107100.v1.1 MD02G1107300.v1.1 MD05G1144900.v1.1 MD10G1144700.v1.1 MD15G1226500.v1.1
prunus_persica Prupe.7G188700_v2.0.a1 Prupe.7G188700_v2.0.a1 Prupe.7G188700_v2.0.a1 Prupe.8G183000_v2.0.a1
pyrus_communis pycom05g14000 pycom10g12440 pycom15g20140
rosa_chinensis RchiOBHm_Chr2g0096621 RchiOBHm_Chr6g0281601 RchiOBHm_Chr6g0281651 RchiOBHm_Chr7g0212121
rosa_laevigata RLG00000002926 RLG00000002927 RLG00000002933 RLG00000008638 RLG00000012954 RLG00000012955 RLG00000016656
rosa_multiflora Rmu_co8091766.1_g000001 Rmu_co8287847.1_g000001 Rmu_co8297221.1_g000001 Rmu_co8485327.1_g000001 Rmu_co8512767.1_g000001 Rmu_sc0002316.1_g000041 Rmu_sc0003565.1_g000009 Rmu_sc0014556.1_g000004 Rmu_sc0027573.1_g000001 Rmu_sc0030028.1_g000001
rosa_roxburghii Rroxscaffold_1G00026540 Rroxscaffold_2G00145470 Rroxscaffold_3G00247100 Rroxscaffold_3G00247110 Rroxscaffold_3G00247120 Rroxscaffold_5G00362680 Rroxscaffold_5G00362710 Rroxscaffold_7G00186950
rosa_rugosa Rorug02G0059000 Rorug02G0059100 Rorug06G0142300 Rorug07G0131000 Rorug07G0131100
rosa_samantha Rh1DG155700 Rh2AG106500 Rh2AG171500 Rh2BG108800 Rh2BG178900 Rh2CG110600 Rh2CG176400 Rh2DG110100 Rh2DG177600 Rh4BG120600 Rh6BG258300 Rh6CG257100 Rh6DG249200 Rh6DG249300 Rh7AG264100 Rh7AG264200 Rh7BG257800 Rh7BG257900 Rh7BG258000 Rh7BG258100 Rh7CG280600 Rh7CG280700 Rh7DG270600
rosa_wichuraiana Rw2G008240 Rw6G022110 Rw7G022540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 353
AatII GACGTC 1 cut(s) 2024
AccB1I GGYRCC 3 cut(s) 568, 932, 1293
AccI GTMKAC 2 cut(s) 1143, 1323
AccII CGCG 7 cut(s) 599, 809, 1031, 1523, 1525, 1549, 1551
AccIII TCCGGA 1 cut(s) 514
AclWI GGATC 6 cut(s) 92, 224, 506, 596, 625, 638
AcoI YGGCCR 3 cut(s) 672, 1233, 1709
AcuI CTGAAG 2 cut(s) 143, 175
AcyI GRCGYC 2 cut(s) 1007, 2021
AdeI CACNNNGTG 1 cut(s) 475
AfaI GTAC 7 cut(s) 224, 986, 1331, 1406, 1457, 1613, 1667
AfiI CCNNNNNNNGG 6 cut(s) 364, 365, 1180, 1392, 2055, 2077
AflII CTTAAG 2 cut(s) 1034, 1079
AflIII ACRYGT 1 cut(s) 1662
AgsI TTSAA 6 cut(s) 292, 499, 913, 1519, 1910, 1954
AhdI GACNNNNNGTC 1 cut(s) 646
AjiI CACGTC 1 cut(s) 1663
AjnI CCWGG 3 cut(s) 604, 2027, 2112
AjuI GAANNNNNNNTTGG 4 cut(s) 173, 205, 1890, 1922
AleI CACNNNNGTG 1 cut(s) 1421
Alw21I GWGCWC 3 cut(s) 157, 489, 1242
Alw26I GTCTC 3 cut(s) 326, 1215, 1473
AlwI GGATC 6 cut(s) 92, 224, 506, 596, 625, 638
Ama87I CYCGRG 2 cut(s) 1597, 1938
Aor13HI TCCGGA 1 cut(s) 514
ApeKI GCWGC 1 cut(s) 1995
ArsI GACNNNNNNTTYG 2 cut(s) 1260, 1292
Asp700I GAANNNNTTC 1 cut(s) 1193
AspA2I CCTAGG 2 cut(s) 1387, 1528
AspLEI GCGC 9 cut(s) 416, 601, 1031, 1033, 1525, 1527, 1549, 1551, 1553
AspS9I GGNCC 6 cut(s) 89, 732, 1012, 1370, 1831, 1887
AsuHPI GGTGA 5 cut(s) 16, 40, 82, 229, 1234
AsuNHI GCTAGC 2 cut(s) 1156, 1490
AvaI CYCGRG 2 cut(s) 1597, 1938
AvaII GGWCC 2 cut(s) 89, 1012
AvrII CCTAGG 2 cut(s) 1387, 1528
BaeGI GKGCMC 1 cut(s) 1604
BalI TGGCCA 1 cut(s) 674
BamHI GGATCC 1 cut(s) 630
BanI GGYRCC 3 cut(s) 568, 932, 1293
BanII GRGCYC 4 cut(s) 157, 489, 1242, 2072
BauI CACGAG 1 cut(s) 341
Bbv12I GWGCWC 3 cut(s) 157, 489, 1242
BbvCI CCTCAGC 2 cut(s) 128, 1429
BbvI GCAGC 1 cut(s) 2007
BccI CCATC 4 cut(s) 1253, 1386, 1600, 1875
BceAI ACGGC 3 cut(s) 338, 1696, 1724
BciT130I CCWGG 3 cut(s) 606, 2029, 2114
BciVI GTATCC 2 cut(s) 269, 1724
BcoDI GTCTC 3 cut(s) 326, 1215, 1473
BfaI CTAG 7 cut(s) 923, 1157, 1388, 1491, 1529, 1688, 1742
BfmI CTRYAG 1 cut(s) 1902
BfoI RGCGCY 1 cut(s) 417
BfrI CTTAAG 2 cut(s) 1034, 1079
BfuI GTATCC 2 cut(s) 269, 1724
BisI GCNGC 4 cut(s) 42, 572, 807, 1996
BlnI CCTAGG 2 cut(s) 1387, 1528
BlsI GCNGC 4 cut(s) 43, 573, 808, 1997
Bme18I GGWCC 2 cut(s) 89, 1012
BmeRI GACNNNNNGTC 1 cut(s) 646
BmeT110I CYCGRG 2 cut(s) 1597, 1938
BmgBI CACGTC 1 cut(s) 1663
BmgT120I GGNCC 6 cut(s) 89, 732, 1012, 1370, 1831, 1887
BmiI GGNNCC 8 cut(s) 91, 570, 632, 934, 1014, 1295, 1929, 2069
BmsI GCATC 3 cut(s) 345, 378, 1299
BmtI GCTAGC 2 cut(s) 1160, 1494
BpmI CTGGAG 1 cut(s) 1805
Bpu10I CCTNAGC 4 cut(s) 128, 1002, 1161, 1429
BpuEI CTTGAG 3 cut(s) 752, 1183, 1469
BsaAI YACGTR 1 cut(s) 1819
BsaBI GATNNNNATC 1 cut(s) 593
BsaHI GRCGYC 2 cut(s) 1007, 2021
BsaI GGTCTC 1 cut(s) 1473
BsaWI WCCGGW 3 cut(s) 514, 815, 1278
BsaXI ACNNNNNCTCC 8 cut(s) 332, 362, 699, 729, 1349, 1379, 1428, 1458
Bsc4I CCNNNNNNNGG 6 cut(s) 364, 365, 1180, 1392, 2055, 2077
Bse118I RCCGGY 1 cut(s) 416
Bse1I ACTGG 3 cut(s) 82, 229, 1931
Bse3DI GCAATG 1 cut(s) 1998
Bse8I GATNNNNATC 1 cut(s) 593
BseAI TCCGGA 1 cut(s) 514
BseBI CCWGG 3 cut(s) 606, 2029, 2114
BseGI GGATG 6 cut(s) 336, 825, 1314, 1397, 1867, 2046
BseJI GATNNNNATC 1 cut(s) 593
BseLI CCNNNNNNNGG 6 cut(s) 364, 365, 1180, 1392, 2055, 2077
BseMI GCAATG 1 cut(s) 1998
BseMII CTCAG 4 cut(s) 71, 142, 1443, 2052
BseNI ACTGG 3 cut(s) 82, 229, 1931
BsePI GCGCGC 4 cut(s) 1029, 1523, 1547, 1549
BseRI GAGGAG 1 cut(s) 1841
BseSI GKGCMC 1 cut(s) 1604
BseX3I CGGCCG 1 cut(s) 1709
BseXI GCAGC 1 cut(s) 2007
BseYI CCCAGC 1 cut(s) 403
Bsh1236I CGCG 7 cut(s) 599, 809, 1031, 1523, 1525, 1549, 1551
Bsh1285I CGRYCG 1 cut(s) 1712
BshNI GGYRCC 3 cut(s) 568, 932, 1293
BsiEI CGRYCG 1 cut(s) 1712
BsiHKAI GWGCWC 3 cut(s) 157, 489, 1242
BsiHKCI CYCGRG 2 cut(s) 1597, 1938
BslFI GGGAC 7 cut(s) 75, 341, 908, 1217, 1580, 1919, 1928
BslI CCNNNNNNNGG 6 cut(s) 364, 365, 1180, 1392, 2055, 2077
BsmAI GTCTC 3 cut(s) 326, 1215, 1473
BsmBI CGTCTC 2 cut(s) 326, 1215
BsmFI GGGAC 7 cut(s) 75, 341, 908, 1217, 1580, 1919, 1928
Bso31I GGTCTC 1 cut(s) 1473
BsoBI CYCGRG 2 cut(s) 1597, 1938
Bsp1286I GDGCHC 5 cut(s) 157, 489, 1242, 1604, 2072
Bsp13I TCCGGA 1 cut(s) 514
Bsp1407I TGTACA 2 cut(s) 222, 984
BspCNI CTCAG 4 cut(s) 70, 141, 1442, 2053
BspEI TCCGGA 1 cut(s) 514
BspFNI CGCG 7 cut(s) 599, 809, 1031, 1523, 1525, 1549, 1551
BspLI GGNNCC 8 cut(s) 91, 570, 632, 934, 1014, 1295, 1929, 2069
BspOI GCTAGC 2 cut(s) 1160, 1494
BspPI GGATC 6 cut(s) 92, 224, 506, 596, 625, 638
BspQI GCTCTTC 2 cut(s) 162, 2113
BspT107I GGYRCC 3 cut(s) 568, 932, 1293
BspTI CTTAAG 2 cut(s) 1034, 1079
BspTNI GGTCTC 1 cut(s) 1473
BsrDI GCAATG 1 cut(s) 1998
BsrFI RCCGGY 1 cut(s) 416
BsrGI TGTACA 2 cut(s) 222, 984
BsrI ACTGG 3 cut(s) 82, 229, 1931
BssAI RCCGGY 1 cut(s) 416
BssHII GCGCGC 4 cut(s) 1029, 1523, 1547, 1549
BssNAI GTATAC 1 cut(s) 1144
BssNI GRCGYC 2 cut(s) 1007, 2021
BssSI CACGAG 1 cut(s) 341
BssT1I CCWWGG 4 cut(s) 1055, 1123, 1387, 1528
Bst1107I GTATAC 1 cut(s) 1144
Bst2BI CACGAG 1 cut(s) 341
Bst2UI CCWGG 3 cut(s) 606, 2029, 2114
Bst4CI ACNGT 6 cut(s) 455, 568, 644, 862, 957, 2011
Bst6I CTCTTC 3 cut(s) 162, 268, 2113
BstACI GRCGYC 2 cut(s) 1007, 2021
BstAFI CTTAAG 2 cut(s) 1034, 1079
BstAUI TGTACA 2 cut(s) 222, 984
BstBAI YACGTR 1 cut(s) 1819
BstC8I GCNNGC 7 cut(s) 1031, 1158, 1492, 1525, 1549, 1551, 1857
BstDEI CTNAG 7 cut(s) 57, 128, 148, 1002, 1161, 1429, 2061
BstDSI CCRYGG 1 cut(s) 1712
BstEII GGTNACC 1 cut(s) 455
BstENI CCTNNNNNAGG 1 cut(s) 2053
BstF5I GGATG 6 cut(s) 336, 825, 1314, 1397, 1867, 2046
BstFNI CGCG 7 cut(s) 599, 809, 1031, 1523, 1525, 1549, 1551
BstH2I RGCGCY 1 cut(s) 417
BstHHI GCGC 9 cut(s) 416, 601, 1031, 1033, 1525, 1527, 1549, 1551, 1553
BstMAI GTCTC 3 cut(s) 326, 1215, 1473
BstMCI CGRYCG 1 cut(s) 1712
BstMWI GCNNNNNNNGC 7 cut(s) 192, 413, 1157, 1539, 1548, 1852, 1978
BstNI CCWGG 3 cut(s) 606, 2029, 2114
BstNSI RCATGY 2 cut(s) 1336, 1653
BstPI GGTNACC 1 cut(s) 455
BstSFI CTRYAG 1 cut(s) 1902
BstSLI GKGCMC 1 cut(s) 1604
BstSNI TACGTA 1 cut(s) 1819
BstUI CGCG 7 cut(s) 599, 809, 1031, 1523, 1525, 1549, 1551
BstV1I GCAGC 1 cut(s) 2007
BstX2I RGATCY 2 cut(s) 216, 630
BstXI CCANNNNNNTGG 2 cut(s) 472, 1304
BstYI RGATCY 2 cut(s) 216, 630
BstZ17I GTATAC 1 cut(s) 1144
BstZI CGGCCG 1 cut(s) 1709
BsuI GTATCC 2 cut(s) 269, 1724
BtgI CCRYGG 1 cut(s) 1712
BtgZI GCGATG 2 cut(s) 355, 1600
BtrI CACGTC 1 cut(s) 1663
BtsCI GGATG 6 cut(s) 336, 825, 1314, 1397, 1867, 2046
BtsI GCAGTG 1 cut(s) 1191
BtsIMutI CAGTG 1 cut(s) 1191
Cac8I GCNNGC 7 cut(s) 1031, 1158, 1492, 1525, 1549, 1551, 1857
CfoI GCGC 9 cut(s) 416, 601, 1031, 1033, 1525, 1527, 1549, 1551, 1553
Cfr10I RCCGGY 1 cut(s) 416
Cfr13I GGNCC 6 cut(s) 89, 732, 1012, 1370, 1831, 1887
Cfr9I CCCGGG 1 cut(s) 1597
CseI GACGC 3 cut(s) 62, 798, 996
Csp6I GTAC 7 cut(s) 223, 985, 1330, 1405, 1456, 1612, 1666
CspCI CAANNNNNGTGG 2 cut(s) 1694, 1729
CviAII CATG 5 cut(s) 529, 670, 1333, 1650, 1978
CviQI GTAC 7 cut(s) 223, 985, 1330, 1405, 1456, 1612, 1666
DdeI CTNAG 7 cut(s) 57, 128, 148, 1002, 1161, 1429, 2061
DraIII CACNNNGTG 1 cut(s) 475
DrdI GACNNNNNNGTC 1 cut(s) 353
DriI GACNNNNNGTC 1 cut(s) 646
DseDI GACNNNNNNGTC 1 cut(s) 353
EaeI YGGCCR 3 cut(s) 672, 1233, 1709
EagI CGGCCG 1 cut(s) 1709
Eam1104I CTCTTC 3 cut(s) 162, 268, 2113
Eam1105I GACNNNNNGTC 1 cut(s) 646
EarI CTCTTC 3 cut(s) 162, 268, 2113
EciI GGCGGA 3 cut(s) 166, 340, 1743
Ecl136II GAGCTC 3 cut(s) 155, 487, 1240
EclXI CGGCCG 1 cut(s) 1709
Eco105I TACGTA 1 cut(s) 1819
Eco130I CCWWGG 4 cut(s) 1055, 1123, 1387, 1528
Eco24I GRGCYC 4 cut(s) 157, 489, 1242, 2072
Eco31I GGTCTC 1 cut(s) 1473
Eco47I GGWCC 2 cut(s) 89, 1012
Eco52I CGGCCG 1 cut(s) 1709
Eco53kI GAGCTC 3 cut(s) 155, 487, 1240
Eco57I CTGAAG 2 cut(s) 143, 175
Eco88I CYCGRG 2 cut(s) 1597, 1938
Eco91I GGTNACC 1 cut(s) 455
EcoICRI GAGCTC 3 cut(s) 155, 487, 1240
EcoNI CCTNNNNNAGG 1 cut(s) 2053
EcoO65I GGTNACC 1 cut(s) 455
EcoRII CCWGG 3 cut(s) 604, 2027, 2112
EcoT14I CCWWGG 4 cut(s) 1055, 1123, 1387, 1528
EcoT38I GRGCYC 4 cut(s) 157, 489, 1242, 2072
ErhI CCWWGG 4 cut(s) 1055, 1123, 1387, 1528
Esp3I CGTCTC 2 cut(s) 326, 1215
FaeI CATG 5 cut(s) 532, 673, 1336, 1653, 1981
FalI AAGNNNNNCTT 4 cut(s) 491, 523, 779, 811
FaqI GGGAC 7 cut(s) 75, 341, 908, 1217, 1580, 1919, 1928
FatI CATG 5 cut(s) 528, 669, 1332, 1649, 1977
FauI CCCGC 1 cut(s) 142
FblI GTMKAC 2 cut(s) 1143, 1323
Fnu4HI GCNGC 4 cut(s) 42, 572, 807, 1996
FokI GGATG 6 cut(s) 323, 812, 1321, 1404, 1854, 2053
FriOI GRGCYC 4 cut(s) 157, 489, 1242, 2072
Fsp4HI GCNGC 4 cut(s) 42, 572, 807, 1996
FspBI CTAG 7 cut(s) 923, 1157, 1388, 1491, 1529, 1688, 1742
GlaI GCGC 9 cut(s) 415, 600, 1030, 1032, 1524, 1526, 1548, 1550, 1552
GluI GCNGC 4 cut(s) 42, 572, 807, 1996
GsaI CCCAGC 1 cut(s) 407
GsuI CTGGAG 1 cut(s) 1805
HaeII RGCGCY 1 cut(s) 417
HgaI GACGC 3 cut(s) 62, 798, 996
HhaI GCGC 9 cut(s) 416, 601, 1031, 1033, 1525, 1527, 1549, 1551, 1553
Hin1I GRCGYC 2 cut(s) 1007, 2021
Hin1II CATG 5 cut(s) 532, 673, 1336, 1653, 1981
Hin6I GCGC 9 cut(s) 414, 599, 1029, 1031, 1523, 1525, 1547, 1549, 1551
HinP1I GCGC 9 cut(s) 414, 599, 1029, 1031, 1523, 1525, 1547, 1549, 1551
HincII GTYRAC 3 cut(s) 781, 1706, 2014
HindII GTYRAC 3 cut(s) 781, 1706, 2014
HinfI GANTC 8 cut(s) 7, 173, 260, 347, 853, 868, 1655, 1941
HpaI GTTAAC 1 cut(s) 2014
HphI GGTGA 5 cut(s) 16, 40, 82, 229, 1234
Hpy188I TCNGA 9 cut(s) 60, 162, 253, 690, 800, 1012, 1660, 2053, 2062
Hpy188III TCNNGA 8 cut(s) 244, 257, 515, 592, 628, 1556, 1688, 2038
Hpy99I CGWCG 8 cut(s) 56, 284, 455, 644, 1012, 1106, 2023, 2026
HpyCH4III ACNGT 6 cut(s) 455, 568, 644, 862, 957, 2011
HpyCH4IV ACGT 5 cut(s) 279, 312, 1662, 1818, 2021
HpyCH4V TGCA 1 cut(s) 1765
HpyF10VI GCNNNNNNNGC 7 cut(s) 192, 413, 1157, 1539, 1548, 1852, 1978
HpyF3I CTNAG 7 cut(s) 57, 128, 148, 1002, 1161, 1429, 2061
HpySE526I ACGT 5 cut(s) 279, 312, 1662, 1818, 2021
Hsp92I GRCGYC 2 cut(s) 1007, 2021
Hsp92II CATG 5 cut(s) 532, 673, 1336, 1653, 1981
HspAI GCGC 9 cut(s) 414, 599, 1029, 1031, 1523, 1525, 1547, 1549, 1551
Kpn2I TCCGGA 1 cut(s) 514
KspAI GTTAAC 1 cut(s) 2014
LguI GCTCTTC 2 cut(s) 162, 2113
LmnI GCTCC 5 cut(s) 152, 335, 707, 1344, 2067
Lsp1109I GCAGC 1 cut(s) 2007
LweI GCATC 3 cut(s) 345, 378, 1299
MaeI CTAG 7 cut(s) 923, 1157, 1388, 1491, 1529, 1688, 1742
MaeII ACGT 5 cut(s) 279, 312, 1662, 1818, 2021
MaeIII GTNAC 7 cut(s) 455, 648, 821, 1067, 1281, 1414, 2099
MboII GAAGA 6 cut(s) 149, 255, 258, 925, 1201, 2100
MfeI CAATTG 2 cut(s) 480, 1872
MflI RGATCY 2 cut(s) 216, 630
MhlI GDGCHC 5 cut(s) 157, 489, 1242, 1604, 2072
MlsI TGGCCA 1 cut(s) 674
MluCI AATT 6 cut(s) 480, 744, 1115, 1736, 1821, 1872
MluNI TGGCCA 1 cut(s) 674
MlyI GAGTC 5 cut(s) 167, 254, 341, 1664, 1950
MmeI TCCRAC 2 cut(s) 125, 990
Mox20I TGGCCA 1 cut(s) 674
MroI TCCGGA 1 cut(s) 514
MroXI GAANNNNTTC 1 cut(s) 1193
MscI TGGCCA 1 cut(s) 674
MslI CAYNNNNRTG 2 cut(s) 1302, 1421
Msp20I TGGCCA 1 cut(s) 674
MspCI CTTAAG 2 cut(s) 1034, 1079
MunI CAATTG 2 cut(s) 480, 1872
MvaI CCWGG 3 cut(s) 606, 2029, 2114
MvnI CGCG 7 cut(s) 599, 809, 1031, 1523, 1525, 1549, 1551
MwoI GCNNNNNNNGC 7 cut(s) 192, 413, 1157, 1539, 1548, 1852, 1978
NheI GCTAGC 2 cut(s) 1156, 1490
NlaIII CATG 5 cut(s) 532, 673, 1336, 1653, 1981
NlaIV GGNNCC 8 cut(s) 91, 570, 632, 934, 1014, 1295, 1929, 2069
NmeAIII GCCGAG 2 cut(s) 709, 1261
NmuCI GTSAC 3 cut(s) 648, 1414, 2099
NspI RCATGY 2 cut(s) 1336, 1653
OliI CACNNNNGTG 1 cut(s) 1421
PauI GCGCGC 4 cut(s) 1029, 1523, 1547, 1549
PciSI GCTCTTC 2 cut(s) 162, 2113
PcsI WCGNNNNNNNCGW 3 cut(s) 447, 1584, 2015
PdmI GAANNNNTTC 1 cut(s) 1193
PfeI GAWTC 3 cut(s) 7, 853, 868
PflFI GACNNNGTC 2 cut(s) 776, 2023
PfoI TCCNGGA 2 cut(s) 271, 1045
PkrI GCNGC 4 cut(s) 43, 573, 808, 1997
PleI GAGTC 5 cut(s) 167, 254, 341, 1663, 1949
PpsI GAGTC 5 cut(s) 167, 254, 341, 1663, 1949
Ppu21I YACGTR 1 cut(s) 1819
Psp124BI GAGCTC 3 cut(s) 157, 489, 1242
Psp6I CCWGG 3 cut(s) 604, 2027, 2112
PspEI GGTNACC 1 cut(s) 455
PspFI CCCAGC 1 cut(s) 403
PspGI CCWGG 3 cut(s) 604, 2027, 2112
PspN4I GGNNCC 8 cut(s) 91, 570, 632, 934, 1014, 1295, 1929, 2069
PspPI GGNCC 6 cut(s) 89, 732, 1012, 1370, 1831, 1887
PsuI RGATCY 2 cut(s) 216, 630
PsyI GACNNNGTC 2 cut(s) 776, 2023
PteI GCGCGC 4 cut(s) 1029, 1523, 1547, 1549
RsaI GTAC 7 cut(s) 224, 986, 1331, 1406, 1457, 1613, 1667
RsaNI GTAC 7 cut(s) 223, 985, 1330, 1405, 1456, 1612, 1666
RseI CAYNNNNRTG 2 cut(s) 1302, 1421
SacI GAGCTC 3 cut(s) 157, 489, 1242
SapI GCTCTTC 2 cut(s) 162, 2113
SatI GCNGC 4 cut(s) 42, 572, 807, 1996
Sau96I GGNCC 6 cut(s) 89, 732, 1012, 1370, 1831, 1887
SchI GAGTC 5 cut(s) 167, 254, 341, 1664, 1950
SduI GDGCHC 5 cut(s) 157, 489, 1242, 1604, 2072
SfaNI GCATC 3 cut(s) 345, 378, 1299
SfcI CTRYAG 1 cut(s) 1902
SinI GGWCC 2 cut(s) 89, 1012
SmaI CCCGGG 1 cut(s) 1599
SmiMI CAYNNNNRTG 2 cut(s) 1302, 1421
SmlI CTYRAG 5 cut(s) 767, 1034, 1079, 1198, 1484
SmoI CTYRAG 5 cut(s) 767, 1034, 1079, 1198, 1484
SnaBI TACGTA 1 cut(s) 1819
Sse9I AATT 6 cut(s) 480, 744, 1115, 1736, 1821, 1872
SspMI CTAG 7 cut(s) 923, 1157, 1388, 1491, 1529, 1688, 1742
SstI GAGCTC 3 cut(s) 157, 489, 1242
StyI CCWWGG 4 cut(s) 1055, 1123, 1387, 1528
TaaI ACNGT 6 cut(s) 455, 568, 644, 862, 957, 2011
TaiI ACGT 5 cut(s) 282, 315, 1665, 1821, 2024
TaqI TCGA 7 cut(s) 258, 489, 510, 591, 1101, 1176, 1587
TaqII GACCGA 2 cut(s) 77, 698
TasI AATT 6 cut(s) 480, 744, 1115, 1736, 1821, 1872
TatI WGTACW 3 cut(s) 222, 984, 1329
TauI GCSGC 3 cut(s) 44, 574, 809
TfiI GAWTC 3 cut(s) 7, 853, 868
TscAI CASTG 1 cut(s) 1191
TseFI GTSAC 3 cut(s) 648, 1414, 2099
TseI GCWGC 1 cut(s) 1995
Tsp45I GTSAC 3 cut(s) 648, 1414, 2099
TspDTI ATGAA 3 cut(s) 367, 658, 1578
TspGWI ACGGA 1 cut(s) 626
TspMI CCCGGG 1 cut(s) 1597
TspRI CASTG 1 cut(s) 1191
Tth111I GACNNNGTC 2 cut(s) 776, 2023
Vha464I CTTAAG 2 cut(s) 1034, 1079
VpaK11BI GGWCC 2 cut(s) 89, 1012
XagI CCTNNNNNAGG 1 cut(s) 2053
XbaI TCTAGA 1 cut(s) 1687
XceI RCATGY 2 cut(s) 1336, 1653
XcmI CCANNNNNNNNNTGG 3 cut(s) 469, 472, 641
XmaI CCCGGG 1 cut(s) 1597
XmaJI CCTAGG 2 cut(s) 1387, 1528
XmiI GTMKAC 2 cut(s) 1143, 1323
XmnI GAANNNNTTC 1 cut(s) 1193
XspI CTAG 7 cut(s) 923, 1157, 1388, 1491, 1529, 1688, 1742
ZraI GACGTC 1 cut(s) 2022
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.