RLG00000002926

Calmodulin-binding receptor-like cytoplasmic kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0000688
Physical Location & Seq
Forward (+)
41840956 .. 41841709
754 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000002926

Sequence Viewer

Length: 513 bp
ATGGTGAGAAGTGGAAATGAAGCAGTAAAGGGGCAGACAAAGAAAGAGTTCTTTGCCTCTTTGGTAGACGTGGACAGGCCAGATTCATTCGAGAGTCAGATACGGGAGTTTAATGATGAACTGAGTCTCCCCAACCATGACATAGCTGATCTCGAAACACAAATGATCGAACGCGCACAACTTGTTGGCAGGATGTCTGGTTTTCATATGTACAACTATTGCTGCATGGATAGTTGTGACGACCAACTTTATGAATTAAATAGGTCTCTTGATAGACTTTTAGACAACTTGCAGCTGCAGCAAGCAAGGGATGTGAAGGAGATCTTGAAACTGTCTAGGCAAAACCGCAACGATCTTGATGAAATGAAGGGTATGTTGAAGATGACACTGCAGCAGATCAATGATCCTGAAATGAAGGGTCTAGTGAAGAAGTTACTGGAGCAGAATCCAGGGGATTCAACAACAGAGACCTCAAGTTCTGCTTCAGAAGTAATACAGAAGAGAAGGGAATAA

Protein Analysis

171

Amino Acids

19.65

Weight (kDa)

4.91

Isoelectric Point (pI)

51.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 32 - 121 4.8e-09 Arabidopsis broad-spectrum mildew resistance protein RPW8
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000688)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g09820 FvH4_2g17160 FvH4_2g17160
malus_domestica MD02G1107100.v1.1 MD02G1107300.v1.1 MD05G1144900.v1.1 MD10G1144700.v1.1 MD15G1226500.v1.1
prunus_persica Prupe.7G188700_v2.0.a1 Prupe.7G188700_v2.0.a1 Prupe.7G188700_v2.0.a1 Prupe.8G183000_v2.0.a1
pyrus_communis pycom05g14000 pycom10g12440 pycom15g20140
rosa_chinensis RchiOBHm_Chr2g0096621 RchiOBHm_Chr6g0281601 RchiOBHm_Chr6g0281651 RchiOBHm_Chr7g0212121
rosa_laevigata RLG00000002926 RLG00000002927 RLG00000002933 RLG00000008638 RLG00000012954 RLG00000012955 RLG00000016656
rosa_multiflora Rmu_co8091766.1_g000001 Rmu_co8287847.1_g000001 Rmu_co8297221.1_g000001 Rmu_co8485327.1_g000001 Rmu_co8512767.1_g000001 Rmu_sc0002316.1_g000041 Rmu_sc0003565.1_g000009 Rmu_sc0014556.1_g000004 Rmu_sc0027573.1_g000001 Rmu_sc0030028.1_g000001
rosa_roxburghii Rroxscaffold_1G00026540 Rroxscaffold_2G00145470 Rroxscaffold_3G00247100 Rroxscaffold_3G00247110 Rroxscaffold_3G00247120 Rroxscaffold_5G00362680 Rroxscaffold_5G00362710 Rroxscaffold_7G00186950
rosa_rugosa Rorug02G0059000 Rorug02G0059100 Rorug06G0142300 Rorug07G0131000 Rorug07G0131100
rosa_samantha Rh1DG155700 Rh2AG106500 Rh2AG171500 Rh2BG108800 Rh2BG178900 Rh2CG110600 Rh2CG176400 Rh2DG110100 Rh2DG177600 Rh4BG120600 Rh6BG258300 Rh6CG257100 Rh6DG249200 Rh6DG249300 Rh7AG264100 Rh7AG264200 Rh7BG257800 Rh7BG257900 Rh7BG258000 Rh7BG258100 Rh7CG280600 Rh7CG280700 Rh7DG270600
rosa_wichuraiana Rw2G008240 Rw6G022110 Rw7G022540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 66
AccII CGCG 1 cut(s) 174
AciI CCGC 1 cut(s) 346
AclWI GGATC 1 cut(s) 398
AcuI CTGAAG 1 cut(s) 468
AfaI GTAC 1 cut(s) 212
AgsI TTSAA 3 cut(s) 328, 379, 459
AjiI CACGTC 1 cut(s) 70
AjnI CCWGG 1 cut(s) 448
AloI GAACNNNNNNTCC 2 cut(s) 111, 143
AluBI AGCT 2 cut(s) 146, 295
AluI AGCT 2 cut(s) 146, 295
Alw26I GTCTC 3 cut(s) 131, 270, 461
AlwI GGATC 1 cut(s) 398
AoxI GGCC 1 cut(s) 77
ApeKI GCWGC 5 cut(s) 222, 292, 295, 298, 391
Asp700I GAANNNNTTC 1 cut(s) 47
AspLEI GCGC 1 cut(s) 176
AsuHPI GGTGA 1 cut(s) 16
BaeI ACNNNNGTAYC 2 cut(s) 92, 125
BbvI GCAGC 5 cut(s) 209, 282, 304, 310, 403
BciT130I CCWGG 1 cut(s) 450
BcoDI GTCTC 3 cut(s) 131, 270, 461
BfaI CTAG 2 cut(s) 336, 422
BfmI CTRYAG 2 cut(s) 296, 389
BglII AGATCT 1 cut(s) 321
BisI GCNGC 5 cut(s) 223, 293, 296, 299, 392
BlsI GCNGC 5 cut(s) 224, 294, 297, 300, 393
Bme1390I CCNGG 1 cut(s) 450
BmgBI CACGTC 1 cut(s) 70
BmrFI CCNGG 1 cut(s) 450
BpmI CTGGAG 1 cut(s) 458
BpuEI CTTGAG 1 cut(s) 457
BsaI GGTCTC 2 cut(s) 270, 461
BsaJI CCNNGG 1 cut(s) 449
BsaXI ACNNNNNCTCC 2 cut(s) 111, 141
Bse1I ACTGG 1 cut(s) 441
BseBI CCWGG 1 cut(s) 450
BseDI CCNNGG 1 cut(s) 449
BseGI GGATG 2 cut(s) 198, 316
BseMII CTCAG 1 cut(s) 113
BseNI ACTGG 1 cut(s) 441
BseXI GCAGC 5 cut(s) 209, 282, 304, 310, 403
Bsh1236I CGCG 1 cut(s) 174
BshFI GGCC 1 cut(s) 79
BsmAI GTCTC 3 cut(s) 131, 270, 461
BsnI GGCC 1 cut(s) 79
Bso31I GGTCTC 2 cut(s) 270, 461
Bsp1407I TGTACA 1 cut(s) 210
Bsp143I GATC 6 cut(s) 148, 165, 321, 352, 396, 403
BspACI CCGC 1 cut(s) 346
BspANI GGCC 1 cut(s) 79
BspCNI CTCAG 1 cut(s) 114
BspFNI CGCG 1 cut(s) 174
BspMAI CTGCAG 2 cut(s) 300, 393
BspPI GGATC 1 cut(s) 398
BspTNI GGTCTC 2 cut(s) 270, 461
BsrGI TGTACA 1 cut(s) 210
BsrI ACTGG 1 cut(s) 441
BssECI CCNNGG 1 cut(s) 449
BssMI GATC 6 cut(s) 148, 165, 321, 352, 396, 403
Bst2UI CCWGG 1 cut(s) 450
Bst4CI ACNGT 1 cut(s) 333
Bst6I CTCTTC 1 cut(s) 494
BstAUI TGTACA 1 cut(s) 210
BstC8I GCNNGC 1 cut(s) 303
BstDEI CTNAG 1 cut(s) 122
BstF5I GGATG 2 cut(s) 198, 316
BstFNI CGCG 1 cut(s) 174
BstHHI GCGC 1 cut(s) 176
BstKTI GATC 6 cut(s) 151, 168, 324, 355, 399, 406
BstMAI GTCTC 3 cut(s) 131, 270, 461
BstMBI GATC 6 cut(s) 148, 165, 321, 352, 396, 403
BstMWI GCNNNNNNNGC 1 cut(s) 298
BstNI CCWGG 1 cut(s) 450
BstSCI CCNGG 1 cut(s) 448
BstSFI CTRYAG 2 cut(s) 296, 389
BstUI CGCG 1 cut(s) 174
BstV1I GCAGC 5 cut(s) 209, 282, 304, 310, 403
BstX2I RGATCY 1 cut(s) 321
BstYI RGATCY 1 cut(s) 321
BsuRI GGCC 1 cut(s) 79
BtrI CACGTC 1 cut(s) 70
BtsCI GGATG 2 cut(s) 198, 316
BtsI GCAGTG 1 cut(s) 386
BtsIMutI CAGTG 1 cut(s) 386
Cac8I GCNNGC 1 cut(s) 303
CfoI GCGC 1 cut(s) 176
Csp6I GTAC 1 cut(s) 211
CviAII CATG 2 cut(s) 137, 226
CviJI RGCY 3 cut(s) 79, 146, 295
CviKI_1 RGCY 3 cut(s) 79, 146, 295
CviQI GTAC 1 cut(s) 211
DdeI CTNAG 1 cut(s) 122
DpnI GATC 6 cut(s) 150, 167, 323, 354, 398, 405
DpnII GATC 6 cut(s) 148, 165, 321, 352, 396, 403
Eam1104I CTCTTC 1 cut(s) 494
EarI CTCTTC 1 cut(s) 494
Eco31I GGTCTC 2 cut(s) 270, 461
Eco57I CTGAAG 1 cut(s) 468
EcoRII CCWGG 1 cut(s) 448
FaeI CATG 2 cut(s) 140, 229
FaiI YATR 7 cut(s) 138, 143, 207, 209, 227, 252, 374
FalI AAGNNNNNCTT 4 cut(s) 308, 340, 466, 498
FatI CATG 2 cut(s) 136, 225
FauNDI CATATG 1 cut(s) 207
FblI GTMKAC 1 cut(s) 66
Fnu4HI GCNGC 5 cut(s) 223, 293, 296, 299, 392
FokI GGATG 2 cut(s) 205, 323
Fsp4HI GCNGC 5 cut(s) 223, 293, 296, 299, 392
FspBI CTAG 2 cut(s) 336, 422
GlaI GCGC 1 cut(s) 175
GluI GCNGC 5 cut(s) 223, 293, 296, 299, 392
GsuI CTGGAG 1 cut(s) 458
HaeIII GGCC 1 cut(s) 79
HhaI GCGC 1 cut(s) 176
Hin1II CATG 2 cut(s) 140, 229
Hin6I GCGC 1 cut(s) 174
HinP1I GCGC 1 cut(s) 174
HinfI GANTC 5 cut(s) 83, 94, 124, 445, 455
HphI GGTGA 1 cut(s) 16
Hpy166II GTNNAC 2 cut(s) 67, 73
Hpy188I TCNGA 2 cut(s) 99, 487
Hpy188III TCNNGA 6 cut(s) 91, 152, 269, 325, 356, 407
Hpy8I GTNNAC 2 cut(s) 67, 73
HpyAV CCTTC 4 cut(s) 310, 361, 409, 498
HpyCH4III ACNGT 1 cut(s) 333
HpyCH4IV ACGT 1 cut(s) 69
HpyCH4V TGCA 4 cut(s) 225, 292, 298, 391
HpyF10VI GCNNNNNNNGC 1 cut(s) 298
HpyF3I CTNAG 1 cut(s) 122
HpySE526I ACGT 1 cut(s) 69
Hsp92II CATG 2 cut(s) 140, 229
HspAI GCGC 1 cut(s) 174
Kzo9I GATC 6 cut(s) 148, 165, 321, 352, 396, 403
LmnI GCTCC 1 cut(s) 439
LpnPI CCDG 8 cut(s) 61, 93, 175, 183, 420, 422, 435, 462
Lsp1109I GCAGC 5 cut(s) 209, 282, 304, 310, 403
MaeI CTAG 2 cut(s) 336, 422
MaeII ACGT 1 cut(s) 69
MaeIII GTNAC 2 cut(s) 236, 432
MalI GATC 6 cut(s) 150, 167, 323, 354, 398, 405
MboI GATC 6 cut(s) 148, 165, 321, 352, 396, 403
MboII GAAGA 3 cut(s) 391, 439, 511
MflI RGATCY 1 cut(s) 321
MluCI AATT 1 cut(s) 254
MlyI GAGTC 2 cut(s) 103, 133
MnlI CCTC 2 cut(s) 67, 481
MroXI GAANNNNTTC 1 cut(s) 47
MseI TTAA 2 cut(s) 111, 257
MspA1I CMGCKG 1 cut(s) 295
MspR9I CCNGG 1 cut(s) 450
MvaI CCWGG 1 cut(s) 450
MvnI CGCG 1 cut(s) 174
MwoI GCNNNNNNNGC 1 cut(s) 298
NdeI CATATG 1 cut(s) 207
NdeII GATC 6 cut(s) 148, 165, 321, 352, 396, 403
NlaIII CATG 2 cut(s) 140, 229
NmuCI GTSAC 1 cut(s) 236
PdmI GAANNNNTTC 1 cut(s) 47
PfeI GAWTC 3 cut(s) 83, 445, 455
PkrI GCNGC 5 cut(s) 224, 294, 297, 300, 393
PleI GAGTC 2 cut(s) 102, 132
PpsI GAGTC 2 cut(s) 102, 132
Psp6I CCWGG 1 cut(s) 448
PspGI CCWGG 1 cut(s) 448
PstI CTGCAG 2 cut(s) 300, 393
PsuI RGATCY 1 cut(s) 321
PvuII CAGCTG 1 cut(s) 295
RsaI GTAC 1 cut(s) 212
RsaNI GTAC 1 cut(s) 211
SaqAI TTAA 2 cut(s) 111, 257
SatI GCNGC 5 cut(s) 223, 293, 296, 299, 392
Sau3AI GATC 6 cut(s) 148, 165, 321, 352, 396, 403
SchI GAGTC 2 cut(s) 103, 133
ScrFI CCNGG 1 cut(s) 450
SetI ASST 5 cut(s) 72, 148, 266, 297, 473
SfcI CTRYAG 2 cut(s) 296, 389
SmlI CTYRAG 1 cut(s) 472
SmoI CTYRAG 1 cut(s) 472
Sse9I AATT 1 cut(s) 254
SsiI CCGC 1 cut(s) 346
SspMI CTAG 2 cut(s) 336, 422
StyD4I CCNGG 1 cut(s) 448
TaaI ACNGT 1 cut(s) 333
TaiI ACGT 1 cut(s) 72
TaqI TCGA 3 cut(s) 90, 153, 168
TasI AATT 1 cut(s) 254
TatI WGTACW 1 cut(s) 210
TfiI GAWTC 3 cut(s) 83, 445, 455
Tru1I TTAA 2 cut(s) 111, 257
Tru9I TTAA 2 cut(s) 111, 257
TscAI CASTG 1 cut(s) 393
TseFI GTSAC 1 cut(s) 236
TseI GCWGC 5 cut(s) 222, 292, 295, 298, 391
Tsp45I GTSAC 1 cut(s) 236
TspDTI ATGAA 8 cut(s) 33, 75, 132, 194, 267, 375, 380, 428
TspRI CASTG 1 cut(s) 393
XmiI GTMKAC 1 cut(s) 66
XmnI GAANNNNTTC 1 cut(s) 47
XspI CTAG 2 cut(s) 336, 422
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.