Rroxscaffold_5G00362680

L-type lectin-domain containing receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0000688
Physical Location & Seq
Forward (+)
43704911 .. 43706186
1276 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00362680.1

Sequence Viewer

Length: 924 bp
ATGGTGATTCTGTTTCTCTCCCTTCGTTGTTGCCGGCCGGCGGCGGCTTTCACCGACGCTCGGACCTACAAGGTGAAACCCTACCAGTTCGGATCTTGTACAACCGGTCTTTCACCCTCCGGAACTCGATCTCGACTCTTCTTCTCCGGATACGTCGCCTCTTTCAACTCGTCCTTCCTCATTAACGTCTATTGTCTCAATGGCTCCGCCGTCCCCGGAGAGGGCATCGCCTTCATTATAGCCCCGGATTTGACCATCCCGGCAAACGGCGCAGGTCAATATCTCGGTTTGACCAACGCCACCACCGACGGTAACCCCACCAATCACTTGGTGGCAATTGAGCTCGACACTTTGAAGGAGAGCTTCGATCCGGACGCCAACCACATGGGGCTGAATATCAACTCGGTTAAGTCTAACAAGACGGTGCCACTTTCGGATTACGGAATCGAGATCGCGCCACCAGGCACGGAGTTTTATATGGTGTGGATCCAATACGACGGTGGGTCACATAATCTGTCGGTGTTCATGGCCAACCGTACCGATCGAACAAGGACGGGAAGGGAGCGTTTCTTCCACCGGGAGTTACAGCCAGCTCAACTACGTGTTGATGTGGAATCTAACGGTGGAGATTCGCCGGACGTGGAAGGTGGAGGGGACAACGATAAGGTGTGGAAGATAGCACTAGGGGGAGTTCGTTACCGAGAGCTTAAGAAAGCCACCAATAACTTCGACGACAAGCACAAACTAGGTCAAGGTGGGTTCGGGGTGGTATACCGAGCTGTGCTAGCTAAGGAGGATAACCTCGAAGTGGCGGTGAAGATGTTCTCAAGGGACGAGGTCAAGGGCGAGAGACGACTTTTGGCCGAGCTCACCATCATTAACCGCCTTCGCCACAAACACCTCGTCCGGTTACTCGGTGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lectin_legB Pkinase Pkinase_Tyr

Protein Analysis

307

Amino Acids

33.84

Weight (kDa)

8.27

Isoelectric Point (pI)

36.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lectin_legB PF00139 50 - 181 4.5e-35 Legume lectin domain
Pkinase PF00069 244 - 305 3.1e-09 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 246 - 306 4.7e-09 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000688)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g09820 FvH4_2g17160 FvH4_2g17160
malus_domestica MD02G1107100.v1.1 MD02G1107300.v1.1 MD05G1144900.v1.1 MD10G1144700.v1.1 MD15G1226500.v1.1
prunus_persica Prupe.7G188700_v2.0.a1 Prupe.7G188700_v2.0.a1 Prupe.7G188700_v2.0.a1 Prupe.8G183000_v2.0.a1
pyrus_communis pycom05g14000 pycom10g12440 pycom15g20140
rosa_chinensis RchiOBHm_Chr2g0096621 RchiOBHm_Chr6g0281601 RchiOBHm_Chr6g0281651 RchiOBHm_Chr7g0212121
rosa_laevigata RLG00000002926 RLG00000002927 RLG00000002933 RLG00000008638 RLG00000012954 RLG00000012955 RLG00000016656
rosa_multiflora Rmu_co8091766.1_g000001 Rmu_co8287847.1_g000001 Rmu_co8297221.1_g000001 Rmu_co8485327.1_g000001 Rmu_co8512767.1_g000001 Rmu_sc0002316.1_g000041 Rmu_sc0003565.1_g000009 Rmu_sc0014556.1_g000004 Rmu_sc0027573.1_g000001 Rmu_sc0030028.1_g000001
rosa_roxburghii Rroxscaffold_1G00026540 Rroxscaffold_2G00145470 Rroxscaffold_3G00247100 Rroxscaffold_3G00247110 Rroxscaffold_3G00247120 Rroxscaffold_5G00362680 Rroxscaffold_5G00362710 Rroxscaffold_7G00186950
rosa_rugosa Rorug02G0059000 Rorug02G0059100 Rorug06G0142300 Rorug07G0131000 Rorug07G0131100
rosa_samantha Rh1DG155700 Rh2AG106500 Rh2AG171500 Rh2BG108800 Rh2BG178900 Rh2CG110600 Rh2CG176400 Rh2DG110100 Rh2DG177600 Rh4BG120600 Rh6BG258300 Rh6CG257100 Rh6DG249200 Rh6DG249300 Rh7AG264100 Rh7AG264200 Rh7BG257800 Rh7BG257900 Rh7BG258000 Rh7BG258100 Rh7CG280600 Rh7CG280700 Rh7DG270600
rosa_wichuraiana Rw2G008240 Rw6G022110 Rw7G022540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 263
AccB1I GGYRCC 1 cut(s) 424
AccI GTMKAC 1 cut(s) 771
AccII CGCG 1 cut(s) 455
AccIII TCCGGA 3 cut(s) 119, 146, 370
AciI CCGC 5 cut(s) 41, 44, 207, 812, 883
AclWI GGATC 4 cut(s) 100, 362, 481, 494
AcoI YGGCCR 3 cut(s) 35, 528, 861
AcyI GRCGYC 1 cut(s) 375
AdeI CACNNNGTG 1 cut(s) 331
AfaI GTAC 2 cut(s) 100, 538
AfiI CCNNNNNNNGG 6 cut(s) 40, 60, 220, 221, 266, 808
AflII CTTAAG 1 cut(s) 707
AflIII ACRYGT 1 cut(s) 601
AgeI ACCGGT 1 cut(s) 104
AgsI TTSAA 2 cut(s) 166, 355
AhdI GACNNNNNGTC 1 cut(s) 502
AjiI CACGTC 1 cut(s) 640
AjnI CCWGG 1 cut(s) 460
AluBI AGCT 7 cut(s) 343, 363, 593, 706, 779, 788, 868
AluI AGCT 7 cut(s) 343, 363, 593, 706, 779, 788, 868
Alw21I GWGCWC 2 cut(s) 345, 870
Alw26I GTCTC 2 cut(s) 200, 844
AlwI GGATC 4 cut(s) 100, 362, 481, 494
Aor13HI TCCGGA 3 cut(s) 119, 146, 370
AoxI GGCC 3 cut(s) 35, 528, 861
ArsI GACNNNNNNTTYG 2 cut(s) 888, 920
AsiGI ACCGGT 1 cut(s) 104
Asp700I GAANNNNTTC 1 cut(s) 821
AspLEI GCGC 2 cut(s) 272, 457
AspS9I GGNCC 1 cut(s) 63
AsuC2I CCSGG 4 cut(s) 216, 245, 260, 578
AsuHPI GGTGA 6 cut(s) 16, 43, 85, 105, 826, 862
AsuNHI GCTAGC 1 cut(s) 784
AvaII GGWCC 1 cut(s) 63
BalI TGGCCA 1 cut(s) 530
BamHI GGATCC 1 cut(s) 486
BanI GGYRCC 1 cut(s) 424
BanII GRGCYC 2 cut(s) 345, 870
Bbv12I GWGCWC 2 cut(s) 345, 870
BccI CCATC 2 cut(s) 263, 881
BceAI ACGGC 2 cut(s) 194, 283
BciT130I CCWGG 1 cut(s) 462
BciVI GTATCC 1 cut(s) 143
BcnI CCSGG 4 cut(s) 216, 245, 260, 578
BcoDI GTCTC 2 cut(s) 200, 844
BfaI CTAG 3 cut(s) 683, 746, 785
BfrI CTTAAG 1 cut(s) 707
BfuAI ACCTGC 1 cut(s) 263
BfuI GTATCC 1 cut(s) 143
BisI GCNGC 2 cut(s) 42, 45
BlsI GCNGC 2 cut(s) 43, 46
Bme1390I CCNGG 5 cut(s) 216, 245, 260, 462, 578
Bme18I GGWCC 1 cut(s) 63
BmeRI GACNNNNNGTC 1 cut(s) 502
BmgBI CACGTC 1 cut(s) 640
BmgT120I GGNCC 1 cut(s) 63
BmiI GGNNCC 3 cut(s) 205, 426, 488
BmrFI CCNGG 5 cut(s) 216, 245, 260, 462, 578
BmsI GCATC 1 cut(s) 234
BmtI GCTAGC 1 cut(s) 788
Bpu10I CCTNAGC 1 cut(s) 789
BpuEI CTTGAG 1 cut(s) 811
BpuMI CCSGG 4 cut(s) 216, 245, 260, 578
BsaAI YACGTR 1 cut(s) 602
BsaHI GRCGYC 1 cut(s) 375
BsaJI CCNNGG 2 cut(s) 214, 243
BsaWI WCCGGW 5 cut(s) 104, 119, 146, 370, 906
Bsc4I CCNNNNNNNGG 6 cut(s) 40, 60, 220, 221, 266, 808
Bse118I RCCGGY 3 cut(s) 33, 37, 104
Bse1I ACTGG 1 cut(s) 85
BseAI TCCGGA 3 cut(s) 119, 146, 370
BseBI CCWGG 1 cut(s) 462
BseDI CCNNGG 2 cut(s) 214, 243
BseGI GGATG 1 cut(s) 255
BseLI CCNNNNNNNGG 6 cut(s) 40, 60, 220, 221, 266, 808
BseNI ACTGG 1 cut(s) 85
BseX3I CGGCCG 1 cut(s) 35
Bsh1236I CGCG 1 cut(s) 455
Bsh1285I CGRYCG 2 cut(s) 38, 544
BshFI GGCC 3 cut(s) 37, 530, 863
BshNI GGYRCC 1 cut(s) 424
BshTI ACCGGT 1 cut(s) 104
BsiEI CGRYCG 2 cut(s) 38, 544
BsiHKAI GWGCWC 2 cut(s) 345, 870
BslFI GGGAC 3 cut(s) 197, 668, 845
BslI CCNNNNNNNGG 6 cut(s) 40, 60, 220, 221, 266, 808
BsmAI GTCTC 2 cut(s) 200, 844
BsmBI CGTCTC 1 cut(s) 844
BsmFI GGGAC 3 cut(s) 197, 668, 845
BsnI GGCC 3 cut(s) 37, 530, 863
Bsp1286I GDGCHC 2 cut(s) 345, 870
Bsp13I TCCGGA 3 cut(s) 119, 146, 370
Bsp1407I TGTACA 1 cut(s) 98
Bsp143I GATC 6 cut(s) 92, 128, 367, 450, 486, 541
BspACI CCGC 5 cut(s) 41, 44, 207, 812, 883
BspANI GGCC 3 cut(s) 37, 530, 863
BspEI TCCGGA 3 cut(s) 119, 146, 370
BspFNI CGCG 1 cut(s) 455
BspLI GGNNCC 3 cut(s) 205, 426, 488
BspMI ACCTGC 1 cut(s) 263
BspOI GCTAGC 1 cut(s) 788
BspPI GGATC 4 cut(s) 100, 362, 481, 494
BspT107I GGYRCC 1 cut(s) 424
BspTI CTTAAG 1 cut(s) 707
BsrFI RCCGGY 3 cut(s) 33, 37, 104
BsrGI TGTACA 1 cut(s) 98
BsrI ACTGG 1 cut(s) 85
BssAI RCCGGY 3 cut(s) 33, 37, 104
BssECI CCNNGG 2 cut(s) 214, 243
BssMI GATC 6 cut(s) 92, 128, 367, 450, 486, 541
BssNAI GTATAC 1 cut(s) 772
BssNI GRCGYC 1 cut(s) 375
Bst1107I GTATAC 1 cut(s) 772
Bst2UI CCWGG 1 cut(s) 462
Bst4CI ACNGT 5 cut(s) 311, 424, 500, 536, 623
Bst6I CTCTTC 1 cut(s) 143
BstACI GRCGYC 1 cut(s) 375
BstAFI CTTAAG 1 cut(s) 707
BstAUI TGTACA 1 cut(s) 98
BstBAI YACGTR 1 cut(s) 602
BstC8I GCNNGC 4 cut(s) 35, 39, 591, 786
BstDEI CTNAG 1 cut(s) 789
BstEII GGTNACC 1 cut(s) 311
BstF5I GGATG 1 cut(s) 255
BstFNI CGCG 1 cut(s) 455
BstHHI GCGC 2 cut(s) 272, 457
BstKTI GATC 6 cut(s) 95, 131, 370, 453, 489, 544
BstMAI GTCTC 2 cut(s) 200, 844
BstMBI GATC 6 cut(s) 92, 128, 367, 450, 486, 541
BstMCI CGRYCG 2 cut(s) 38, 544
BstMWI GCNNNNNNNGC 2 cut(s) 269, 785
BstNI CCWGG 1 cut(s) 462
BstPI GGTNACC 1 cut(s) 311
BstSCI CCNGG 5 cut(s) 214, 243, 258, 460, 576
BstUI CGCG 1 cut(s) 455
BstX2I RGATCY 2 cut(s) 92, 486
BstXI CCANNNNNNTGG 2 cut(s) 328, 385
BstYI RGATCY 2 cut(s) 92, 486
BstZ17I GTATAC 1 cut(s) 772
BstZI CGGCCG 1 cut(s) 35
BsuI GTATCC 1 cut(s) 143
BsuRI GGCC 3 cut(s) 37, 530, 863
BtgZI GCGATG 1 cut(s) 211
BtrI CACGTC 1 cut(s) 640
BtsCI GGATG 1 cut(s) 255
BveI ACCTGC 1 cut(s) 263
Cac8I GCNNGC 4 cut(s) 35, 39, 591, 786
CfoI GCGC 2 cut(s) 272, 457
Cfr10I RCCGGY 3 cut(s) 33, 37, 104
Cfr13I GGNCC 1 cut(s) 63
CseI GACGC 2 cut(s) 65, 383
Csp6I GTAC 2 cut(s) 99, 537
CspAI ACCGGT 1 cut(s) 104
CviAII CATG 2 cut(s) 385, 526
CviQI GTAC 2 cut(s) 99, 537
DdeI CTNAG 1 cut(s) 789
DpnI GATC 6 cut(s) 94, 130, 369, 452, 488, 543
DpnII GATC 6 cut(s) 92, 128, 367, 450, 486, 541
DraIII CACNNNGTG 1 cut(s) 331
DriI GACNNNNNGTC 1 cut(s) 502
EaeI YGGCCR 3 cut(s) 35, 528, 861
EagI CGGCCG 1 cut(s) 35
Eam1104I CTCTTC 1 cut(s) 143
Eam1105I GACNNNNNGTC 1 cut(s) 502
EarI CTCTTC 1 cut(s) 143
EciI GGCGGA 1 cut(s) 196
Ecl136II GAGCTC 2 cut(s) 343, 868
EclXI CGGCCG 1 cut(s) 35
Eco24I GRGCYC 2 cut(s) 345, 870
Eco47I GGWCC 1 cut(s) 63
Eco52I CGGCCG 1 cut(s) 35
Eco53kI GAGCTC 2 cut(s) 343, 868
Eco91I GGTNACC 1 cut(s) 311
EcoICRI GAGCTC 2 cut(s) 343, 868
EcoO65I GGTNACC 1 cut(s) 311
EcoRII CCWGG 1 cut(s) 460
EcoT38I GRGCYC 2 cut(s) 345, 870
Esp3I CGTCTC 1 cut(s) 844
FaeI CATG 2 cut(s) 388, 529
FaiI YATR 7 cut(s) 239, 386, 477, 479, 510, 527, 772
FalI AAGNNNNNCTT 2 cut(s) 347, 379
FaqI GGGAC 3 cut(s) 197, 668, 845
FatI CATG 2 cut(s) 384, 525
FblI GTMKAC 1 cut(s) 771
Fnu4HI GCNGC 2 cut(s) 42, 45
FokI GGATG 1 cut(s) 242
FriOI GRGCYC 2 cut(s) 345, 870
Fsp4HI GCNGC 2 cut(s) 42, 45
FspBI CTAG 3 cut(s) 683, 746, 785
GlaI GCGC 2 cut(s) 271, 456
GluI GCNGC 2 cut(s) 42, 45
HaeIII GGCC 3 cut(s) 37, 530, 863
HgaI GACGC 2 cut(s) 65, 383
HhaI GCGC 2 cut(s) 272, 457
Hin1I GRCGYC 1 cut(s) 375
Hin1II CATG 2 cut(s) 388, 529
Hin6I GCGC 2 cut(s) 270, 455
HinP1I GCGC 2 cut(s) 270, 455
HinfI GANTC 5 cut(s) 7, 135, 444, 614, 629
HphI GGTGA 6 cut(s) 16, 43, 85, 105, 826, 862
Hpy166II GTNNAC 1 cut(s) 772
Hpy188I TCNGA 3 cut(s) 63, 92, 436
Hpy188III TCNNGA 5 cut(s) 120, 132, 147, 371, 448
Hpy8I GTNNAC 1 cut(s) 772
Hpy99I CGWCG 5 cut(s) 59, 158, 311, 500, 734
HpyAV CCTTC 7 cut(s) 32, 184, 241, 349, 552, 638, 896
HpyCH4III ACNGT 5 cut(s) 311, 424, 500, 536, 623
HpyCH4IV ACGT 4 cut(s) 153, 186, 601, 639
HpyF10VI GCNNNNNNNGC 2 cut(s) 269, 785
HpyF3I CTNAG 1 cut(s) 789
HpySE526I ACGT 4 cut(s) 153, 186, 601, 639
Hsp92I GRCGYC 1 cut(s) 375
Hsp92II CATG 2 cut(s) 388, 529
HspAI GCGC 2 cut(s) 270, 455
Kpn2I TCCGGA 3 cut(s) 119, 146, 370
KroI GCCGGC 2 cut(s) 33, 37
KroNI GCCGGC 2 cut(s) 35, 39
Kzo9I GATC 6 cut(s) 92, 128, 367, 450, 486, 541
LmnI GCTCC 2 cut(s) 209, 562
LweI GCATC 1 cut(s) 234
MaeI CTAG 3 cut(s) 683, 746, 785
MaeII ACGT 4 cut(s) 153, 186, 601, 639
MaeIII GTNAC 6 cut(s) 311, 504, 582, 695, 909, 917
MalI GATC 6 cut(s) 94, 130, 369, 452, 488, 543
MboI GATC 6 cut(s) 92, 128, 367, 450, 486, 541
MboII GAAGA 5 cut(s) 130, 133, 562, 685, 829
MfeI CAATTG 1 cut(s) 336
MflI RGATCY 2 cut(s) 92, 486
MhlI GDGCHC 2 cut(s) 345, 870
MlsI TGGCCA 1 cut(s) 530
MluCI AATT 1 cut(s) 336
MluNI TGGCCA 1 cut(s) 530
MlyI GAGTC 1 cut(s) 129
MnlI CCTC 9 cut(s) 127, 169, 188, 214, 644, 787, 812, 829, 911
Mox20I TGGCCA 1 cut(s) 530
MroI TCCGGA 3 cut(s) 119, 146, 370
MroNI GCCGGC 2 cut(s) 33, 37
MroXI GAANNNNTTC 1 cut(s) 821
MscI TGGCCA 1 cut(s) 530
MseI TTAA 4 cut(s) 183, 408, 708, 879
Msp20I TGGCCA 1 cut(s) 530
MspCI CTTAAG 1 cut(s) 707
MspR9I CCNGG 5 cut(s) 216, 245, 260, 462, 578
MunI CAATTG 1 cut(s) 336
MvaI CCWGG 1 cut(s) 462
MvnI CGCG 1 cut(s) 455
MwoI GCNNNNNNNGC 2 cut(s) 269, 785
NaeI GCCGGC 2 cut(s) 35, 39
NciI CCSGG 4 cut(s) 216, 245, 260, 578
NdeII GATC 6 cut(s) 92, 128, 367, 450, 486, 541
NgoMIV GCCGGC 2 cut(s) 33, 37
NheI GCTAGC 1 cut(s) 784
NlaIII CATG 2 cut(s) 388, 529
NlaIV GGNNCC 3 cut(s) 205, 426, 488
NmeAIII GCCGAG 1 cut(s) 889
NmuCI GTSAC 2 cut(s) 504, 917
PcsI WCGNNNNNNNCGW 1 cut(s) 303
PdiI GCCGGC 2 cut(s) 35, 39
PdmI GAANNNNTTC 1 cut(s) 821
PfeI GAWTC 4 cut(s) 7, 444, 614, 629
PflFI GACNNNGTC 1 cut(s) 836
PinAI ACCGGT 1 cut(s) 104
PkrI GCNGC 2 cut(s) 43, 46
Ple19I CGATCG 1 cut(s) 544
PleI GAGTC 1 cut(s) 129
PpsI GAGTC 1 cut(s) 129
Ppu21I YACGTR 1 cut(s) 602
Psp124BI GAGCTC 2 cut(s) 345, 870
Psp6I CCWGG 1 cut(s) 460
PspEI GGTNACC 1 cut(s) 311
PspGI CCWGG 1 cut(s) 460
PspN4I GGNNCC 3 cut(s) 205, 426, 488
PspPI GGNCC 1 cut(s) 63
PsuI RGATCY 2 cut(s) 92, 486
PsyI GACNNNGTC 1 cut(s) 836
PvuI CGATCG 1 cut(s) 544
RsaI GTAC 2 cut(s) 100, 538
RsaNI GTAC 2 cut(s) 99, 537
SacI GAGCTC 2 cut(s) 345, 870
SaqAI TTAA 4 cut(s) 183, 408, 708, 879
SatI GCNGC 2 cut(s) 42, 45
Sau3AI GATC 6 cut(s) 92, 128, 367, 450, 486, 541
Sau96I GGNCC 1 cut(s) 63
SchI GAGTC 1 cut(s) 129
ScrFI CCNGG 5 cut(s) 216, 245, 260, 462, 578
SduI GDGCHC 2 cut(s) 345, 870
SfaNI GCATC 1 cut(s) 234
SinI GGWCC 1 cut(s) 63
SmlI CTYRAG 2 cut(s) 707, 826
SmoI CTYRAG 2 cut(s) 707, 826
Sse9I AATT 1 cut(s) 336
SsiI CCGC 5 cut(s) 41, 44, 207, 812, 883
SspMI CTAG 3 cut(s) 683, 746, 785
SstI GAGCTC 2 cut(s) 345, 870
StyD4I CCNGG 5 cut(s) 214, 243, 258, 460, 576
TaaI ACNGT 5 cut(s) 311, 424, 500, 536, 623
TaiI ACGT 4 cut(s) 156, 189, 604, 642
TaqI TCGA 8 cut(s) 127, 133, 345, 366, 447, 544, 729, 804
TasI AATT 1 cut(s) 336
TatI WGTACW 1 cut(s) 98
TauI GCSGC 2 cut(s) 44, 47
TfiI GAWTC 4 cut(s) 7, 444, 614, 629
Tru1I TTAA 4 cut(s) 183, 408, 708, 879
Tru9I TTAA 4 cut(s) 183, 408, 708, 879
TseFI GTSAC 2 cut(s) 504, 917
Tsp45I GTSAC 2 cut(s) 504, 917
TspDTI ATGAA 2 cut(s) 223, 514
TspGWI ACGGA 2 cut(s) 456, 482
Tth111I GACNNNGTC 1 cut(s) 836
Vha464I CTTAAG 1 cut(s) 707
VpaK11BI GGWCC 1 cut(s) 63
XcmI CCANNNNNNNNNTGG 3 cut(s) 325, 328, 497
XmiI GTMKAC 1 cut(s) 771
XmnI GAANNNNTTC 1 cut(s) 821
XspI CTAG 3 cut(s) 683, 746, 785
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.