Rw7G022540

L-type lectin-domain containing receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0000688
Physical Location & Seq
Reverse (-)
26599021 .. 26602257
3237 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G022540.1

Sequence Viewer

Length: 2277 bp
ATGGCCCTGGAGTTAATAGCAGGGTCAGTATTTGGAGTGTTGTATGATCGTGTTTCGCGAGCTGCGGACAGGAAATCAGAGTTCAGATACCTCCTCACTCATATGAAACGCACTCTAGAGTCTTTAACAGGACAGGCCAGATTCATTCGAGAGATACGGGAGTTTAATGATGAATTGAGTCTCCCCAACCATGACATAGCTGATCTTGAAACACAAATGATCGAAGGCGCACAACTCGTTGGCAGGATGTCCAGTTTTCGAATGTACAACTATTGCTGCATGGATGGTTGCCACGACAAACTTTATGAATTGAATAGGTCTCTTGATAGACTTTTAAACAATCTGCAGCTGCAGCAAGCAAGGGATGTGAAGGAGATCTTGAAATTGTCAAGGCAAAACCGTGATGATCTCGATGAAATGAAGGGTATGTTGAAGATGATATTGCAGCAGATCAATGATCCTGAAATGAAGGGTCTAGTGAAGAAGTTACTGGAGCAGAATGCGGGGGACTCGACCGAGAGGTCGACCTCGGCTTCTGCAAGTAATACAGAAAGAGAAGGGAGTGACGAAGCACAAGTGGAATCATTAGAAGGACTATTAGAAGCTGCATTTCAGGTTCTGTTTGATGTCGTTATAGAAGTGAAGGACAAGTCGACTTTGATGCTTGGACCAATTATTGGACATCTCAAATCCACGCTAGACTGCTTAAAGCCATTGATTGAAGATATATCACAGCATAACAACATGGCATTGGATCGCCCAAAGAAGGAACTAGAAACTTTTGGAATGCAAATGAAGAGTGGTGCAGAGCTGGTTCGCGAGTGCTCGGAGGTTCATAAATGGACCAGCAACGAAACATACAAATTCGCCAGCCAACTTTTTGAATTGGATGAGTCTCTTCAAAGGCACTTATGTGTACTAATAGTGCAGGTAGCGAGAGATGTGAAGGAGACCTCGGTTGTGTTAAGGAGTGTACGAGAGGTGGTGGAGAAAATTGAAGGGATAAGTGGTGTGGCAAAAAAACAACAACTTGCATTGGATATGGTGGAGGTGAATGAAATAAGGGATGTGAGGGAGACGACATTGGTTTCAGCAAGGAATGTAGAGGCAGAGTTGACAGTAGTGGATGGCAACGTACCTGAACAACCATCAGTTACAGCTGGATTGGATGTTCCTTCGGAGGAATTGAGTATGTCTAAAAAGAAGAAAAACTCGAAGATGAAGCTTCTTAAGGATGGGTGTCAATGCTTGACCCGACGGTCATTCTACAAGAGGAAGAGGGCTAGGGCCAGGGGAAGGGACTCGAACAGGTTAGACGCTTTGAAGAGTCTTCCAGTGATGTTGAGGGAGTTCAGTTACAGGGAGCTAAAGAAAGCTACCAACAACTTTGACTTGAAGCACACTTTGGGTCAAGGTGGATCTGTGGTTTACAGAGGACTGCTGTCAAAGGAAAATCTTGAAGTGGCAGTGAAGAAGTGCTCCAGGCTAGAGATCAAGGGTAAAGAGGATTTCTTGGCTGACCTCACCATTATTTACCGGCTGCGTCACAAACATATTGTCCCATTGCTTGGTTGGTGTCGCCAAAAGAAGATGCTATTCATAGTCCACGAGTTCATGCCAAATGGCAGCTTAGACAACCACCTATTCTCCGGACCGGAAAATACAACCCTAGAATGGAACCAGAGGTACAAGATCATATCTGGAGTGGCTTCAGTCCTACATTACCTCCAGGAGTTCCATTGCAAAGTAGTCCACAGAAATATCACGGCCAGCCACATAATGCTCGATTCCCACTTCAACGCGCGCCTTGGCAACTTCGGCCTGAGAAGCGCAGAACCCGAAGGTGTGGCGGGCACAATGGGATACATTGCCCCCGAGTGCTTCCACACTGGAGAGATGACCTGCGAGTCCGACGTGTACGGCTTCGGGGCGGTGGTTCTAGAAGTTGTTTGCGGTCAACGACCGTACACCAAAATCGGAGGGTTTCAGGTTTTGGTTGATTGGGTGTGGTCGTTGCATAGAGAAGGACGTGTGCTTGAGGCCGTGGATGAGAGGCTTGGAAATGAGTATGATGTTGAAGAAGCGCAAAGACTTCTGCTTCTTGGGCTGGCCTGCTCTCATCCCATTGCATACGAGAGGCCTAAGAGCCAGACCATAGTCCAGGTTGTTTCCGGGTCTATTCCAGCGCCCAAAGTGTCGCCTTTTAAACCGGCTTTCGTGTGGCCGCCCTTGCCAGAAGGATCGGCAGCATTGCCAATCGACACTGGTAACACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lectin_legB Pkinase Pkinase_Tyr

Protein Analysis

758

Amino Acids

85.67

Weight (kDa)

6.61

Isoelectric Point (pI)

38.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 8 - 139 1.4e-16 Arabidopsis broad-spectrum mildew resistance protein RPW8
RPW8 PF05659 199 - 327 3.2e-21 Arabidopsis broad-spectrum mildew resistance protein RPW8
Pkinase PF00069 464 - 659 1.5e-29 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 465 - 665 3.6e-35 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000688)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g09820 FvH4_2g17160 FvH4_2g17160
malus_domestica MD02G1107100.v1.1 MD02G1107300.v1.1 MD05G1144900.v1.1 MD10G1144700.v1.1 MD15G1226500.v1.1
prunus_persica Prupe.7G188700_v2.0.a1 Prupe.7G188700_v2.0.a1 Prupe.7G188700_v2.0.a1 Prupe.8G183000_v2.0.a1
pyrus_communis pycom05g14000 pycom10g12440 pycom15g20140
rosa_chinensis RchiOBHm_Chr2g0096621 RchiOBHm_Chr6g0281601 RchiOBHm_Chr6g0281651 RchiOBHm_Chr7g0212121
rosa_laevigata RLG00000002926 RLG00000002927 RLG00000002933 RLG00000008638 RLG00000012954 RLG00000012955 RLG00000016656
rosa_multiflora Rmu_co8091766.1_g000001 Rmu_co8287847.1_g000001 Rmu_co8297221.1_g000001 Rmu_co8485327.1_g000001 Rmu_co8512767.1_g000001 Rmu_sc0002316.1_g000041 Rmu_sc0003565.1_g000009 Rmu_sc0014556.1_g000004 Rmu_sc0027573.1_g000001 Rmu_sc0030028.1_g000001
rosa_roxburghii Rroxscaffold_1G00026540 Rroxscaffold_2G00145470 Rroxscaffold_3G00247100 Rroxscaffold_3G00247110 Rroxscaffold_3G00247120 Rroxscaffold_5G00362680 Rroxscaffold_5G00362710 Rroxscaffold_7G00186950
rosa_rugosa Rorug02G0059000 Rorug02G0059100 Rorug06G0142300 Rorug07G0131000 Rorug07G0131100
rosa_samantha Rh1DG155700 Rh2AG106500 Rh2AG171500 Rh2BG108800 Rh2BG178900 Rh2CG110600 Rh2CG176400 Rh2DG110100 Rh2DG177600 Rh4BG120600 Rh6BG258300 Rh6CG257100 Rh6DG249200 Rh6DG249300 Rh7AG264100 Rh7AG264200 Rh7BG257800 Rh7BG257900 Rh7BG258000 Rh7BG258100 Rh7CG280600 Rh7CG280700 Rh7DG270600
rosa_wichuraiana Rw2G008240 Rw6G022110 Rw7G022540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 3 cut(s) 520, 1258, 1906
Acc36I ACCTGC 2 cut(s) 919, 1910
AccB7I CCANNNNNTGG 2 cut(s) 677, 1568
AccI GTMKAC 2 cut(s) 524, 653
AccII CGCG 4 cut(s) 58, 819, 1802, 1804
AccIII TCCGGA 1 cut(s) 1649
AciI CCGC 6 cut(s) 65, 503, 1850, 1931, 1953, 2225
AclWI GGATC 4 cut(s) 452, 762, 1426, 2248
AcoI YGGCCR 2 cut(s) 1767, 2222
AcsI RAATTY 1 cut(s) 863
AcuI CTGAAG 1 cut(s) 1695
AfaI GTAC 7 cut(s) 266, 918, 975, 1137, 1688, 1919, 1967
AfiI CCNNNNNNNGG 5 cut(s) 677, 766, 1296, 1568, 1674
AflII CTTAAG 1 cut(s) 1229
AflIII ACRYGT 2 cut(s) 1914, 2029
AjiI CACGTC 2 cut(s) 1915, 2030
AjnI CCWGG 5 cut(s) 6, 1289, 1481, 1728, 2160
AjuI GAANNNNNNNTTGG 2 cut(s) 1388, 1420
AleI CACNNNNGTG 1 cut(s) 912
AloI GAACNNNNNNTCC 2 cut(s) 1155, 1187
Alw21I GWGCWC 2 cut(s) 827, 1481
Alw26I GTCTC 5 cut(s) 185, 324, 900, 944, 1070
AlwI GGATC 4 cut(s) 452, 762, 1426, 2248
AlwNI CAGNNNCTG 1 cut(s) 619
Ama87I CYCGRG 1 cut(s) 1874
Aor13HI TCCGGA 1 cut(s) 1649
AoxI GGCC 9 cut(s) 3, 135, 1287, 1767, 1819, 2040, 2109, 2138, 2222
ApoI RAATTY 1 cut(s) 863
ArsI GACNNNNNNTTYG 2 cut(s) 1542, 1574
AspLEI GCGC 6 cut(s) 230, 1804, 1806, 1832, 2086, 2188
AspS9I GGNCC 5 cut(s) 4, 668, 843, 1287, 1652
AsuC2I CCSGG 1 cut(s) 2173
AsuHPI GGTGA 2 cut(s) 1063, 1516
AsuII TTCGAA 1 cut(s) 259
AvaI CYCGRG 1 cut(s) 1874
AvaII GGWCC 3 cut(s) 668, 843, 1652
BaeGI GKGCMC 1 cut(s) 1856
BaeI ACNNNNGTAYC 2 cut(s) 146, 179
BauI CACGAG 1 cut(s) 1607
BbsI GAAGAC 1 cut(s) 1322
Bbv12I GWGCWC 2 cut(s) 827, 1481
BccI CCATC 4 cut(s) 278, 1121, 1156, 1229
BceAI ACGGC 3 cut(s) 1782, 1936, 2027
BcgI CGANNNNNNTGC 2 cut(s) 643, 677
BciT130I CCWGG 5 cut(s) 8, 1291, 1483, 1730, 2162
BciVI GTATCC 1 cut(s) 1856
BcnI CCSGG 1 cut(s) 2173
BcoDI GTCTC 5 cut(s) 185, 324, 900, 944, 1070
BfaI CTAG 8 cut(s) 116, 476, 698, 773, 1284, 1487, 1670, 1940
BfmI CTRYAG 2 cut(s) 344, 350
BfoI RGCGCY 1 cut(s) 2189
BfrI CTTAAG 1 cut(s) 1229
BfuAI ACCTGC 2 cut(s) 919, 1910
BfuI GTATCC 1 cut(s) 1856
BglII AGATCT 1 cut(s) 375
Bme1390I CCNGG 6 cut(s) 8, 1291, 1483, 1730, 2162, 2173
Bme18I GGWCC 3 cut(s) 668, 843, 1652
BmeT110I CYCGRG 1 cut(s) 1874
BmgBI CACGTC 2 cut(s) 1915, 2030
BmgT120I GGNCC 5 cut(s) 4, 668, 843, 1287, 1652
BmiI GGNNCC 1 cut(s) 1679
BmrFI CCNGG 6 cut(s) 8, 1291, 1483, 1730, 2162, 2173
BmsI GCATC 2 cut(s) 651, 1581
BoxI GACNNNNGTC 2 cut(s) 1441, 2156
BpiI GAAGAC 1 cut(s) 1322
BpmI CTGGAG 6 cut(s) 29, 512, 1465, 1712, 1722, 1911
Bpu14I TTCGAA 1 cut(s) 259
BpuEI CTTGAG 1 cut(s) 2057
BpuMI CCSGG 1 cut(s) 2173
BsaI GGTCTC 2 cut(s) 324, 944
BsaJI CCNNGG 6 cut(s) 6, 528, 954, 1290, 1807, 2043
BsaWI WCCGGW 2 cut(s) 1649, 1654
BsaXI ACNNNNNCTCC 4 cut(s) 1631, 1661, 1710, 1740
Bsc4I CCNNNNNNNGG 5 cut(s) 677, 766, 1296, 1568, 1674
Bse118I RCCGGY 2 cut(s) 1536, 2209
Bse1I ACTGG 5 cut(s) 252, 495, 1334, 1894, 2269
Bse3DI GCAATG 5 cut(s) 1562, 1738, 1866, 2124, 2249
BseAI TCCGGA 1 cut(s) 1649
BseBI CCWGG 5 cut(s) 8, 1291, 1483, 1730, 2162
BseDI CCNNGG 6 cut(s) 6, 528, 954, 1290, 1807, 2043
BseLI CCNNNNNNNGG 5 cut(s) 677, 766, 1296, 1568, 1674
BseMI GCAATG 5 cut(s) 1562, 1738, 1866, 2124, 2249
BseMII CTCAG 1 cut(s) 1814
BseNI ACTGG 5 cut(s) 252, 495, 1334, 1894, 2269
BsePI GCGCGC 1 cut(s) 1802
BseRI GAGGAG 1 cut(s) 83
BseSI GKGCMC 1 cut(s) 1856
BsgI GTGCAG 2 cut(s) 825, 947
Bsh1236I CGCG 4 cut(s) 58, 819, 1802, 1804
Bsh1285I CGRYCG 2 cut(s) 516, 1964
BshFI GGCC 9 cut(s) 5, 137, 1289, 1769, 1821, 2042, 2111, 2140, 2224
BsiEI CGRYCG 2 cut(s) 516, 1964
BsiHKAI GWGCWC 2 cut(s) 827, 1481
BsiHKCI CYCGRG 1 cut(s) 1874
BsiSI CCGG 5 cut(s) 1537, 1650, 1655, 2172, 2210
BslFI GGGAC 3 cut(s) 521, 1313, 1544
BslI CCNNNNNNNGG 5 cut(s) 677, 766, 1296, 1568, 1674
BsmAI GTCTC 5 cut(s) 185, 324, 900, 944, 1070
BsmBI CGTCTC 1 cut(s) 1070
BsmFI GGGAC 3 cut(s) 521, 1313, 1544
BsmI GAATGC 2 cut(s) 505, 792
BsnI GGCC 9 cut(s) 5, 137, 1289, 1769, 1821, 2042, 2111, 2140, 2224
Bso31I GGTCTC 2 cut(s) 324, 944
BsoBI CYCGRG 1 cut(s) 1874
Bsp119I TTCGAA 1 cut(s) 259
Bsp1286I GDGCHC 3 cut(s) 827, 1481, 1856
Bsp13I TCCGGA 1 cut(s) 1649
Bsp1407I TGTACA 1 cut(s) 264
Bsp68I TCGCGA 2 cut(s) 58, 819
BspACI CCGC 6 cut(s) 65, 503, 1850, 1931, 1953, 2225
BspANI GGCC 9 cut(s) 5, 137, 1289, 1769, 1821, 2042, 2111, 2140, 2224
BspCNI CTCAG 1 cut(s) 1815
BspEI TCCGGA 1 cut(s) 1649
BspFNI CGCG 4 cut(s) 58, 819, 1802, 1804
BspLI GGNNCC 1 cut(s) 1679
BspMAI CTGCAG 2 cut(s) 348, 354
BspMI ACCTGC 2 cut(s) 919, 1910
BspPI GGATC 4 cut(s) 452, 762, 1426, 2248
BspT104I TTCGAA 1 cut(s) 259
BspTI CTTAAG 1 cut(s) 1229
BspTNI GGTCTC 2 cut(s) 324, 944
BsrDI GCAATG 5 cut(s) 1562, 1738, 1866, 2124, 2249
BsrFI RCCGGY 2 cut(s) 1536, 2209
BsrGI TGTACA 1 cut(s) 264
BsrI ACTGG 5 cut(s) 252, 495, 1334, 1894, 2269
BssAI RCCGGY 2 cut(s) 1536, 2209
BssECI CCNNGG 6 cut(s) 6, 528, 954, 1290, 1807, 2043
BssHII GCGCGC 1 cut(s) 1802
BssSI CACGAG 1 cut(s) 1607
BssT1I CCWWGG 1 cut(s) 1807
Bst2BI CACGAG 1 cut(s) 1607
Bst2UI CCWGG 5 cut(s) 8, 1291, 1483, 1730, 2162
Bst4CI ACNGT 4 cut(s) 401, 1120, 1260, 1965
Bst6I CTCTTC 4 cut(s) 791, 903, 1271, 1319
BstAFI CTTAAG 1 cut(s) 1229
BstAUI TGTACA 1 cut(s) 264
BstBI TTCGAA 1 cut(s) 259
BstC8I GCNNGC 8 cut(s) 60, 357, 871, 1771, 1804, 1852, 2109, 2113
BstDEI CTNAG 3 cut(s) 1630, 1823, 2142
BstDSI CCRYGG 1 cut(s) 2043
BstFNI CGCG 4 cut(s) 58, 819, 1802, 1804
BstH2I RGCGCY 1 cut(s) 2189
BstHHI GCGC 6 cut(s) 230, 1804, 1806, 1832, 2086, 2188
BstMAI GTCTC 5 cut(s) 185, 324, 900, 944, 1070
BstMCI CGRYCG 2 cut(s) 516, 1964
BstMWI GCNNNNNNNGC 5 cut(s) 352, 1818, 1827, 2104, 2230
BstNI CCWGG 5 cut(s) 8, 1291, 1483, 1730, 2162
BstPAI GACNNNNGTC 2 cut(s) 1441, 2156
BstSCI CCNGG 6 cut(s) 6, 1289, 1481, 1728, 2160, 2171
BstSFI CTRYAG 2 cut(s) 344, 350
BstSLI GKGCMC 1 cut(s) 1856
BstUI CGCG 4 cut(s) 58, 819, 1802, 1804
BstV2I GAAGAC 1 cut(s) 1322
BstX2I RGATCY 2 cut(s) 375, 1418
BstYI RGATCY 2 cut(s) 375, 1418
BsuI GTATCC 1 cut(s) 1856
BsuRI GGCC 9 cut(s) 5, 137, 1289, 1769, 1821, 2042, 2111, 2140, 2224
BtgI CCRYGG 1 cut(s) 2043
BtrI CACGTC 2 cut(s) 1915, 2030
BtsI GCAGTG 1 cut(s) 1473
BtsIMutI CAGTG 4 cut(s) 1341, 1473, 1887, 2262
BtuMI TCGCGA 2 cut(s) 58, 819
BveI ACCTGC 2 cut(s) 919, 1910
Cac8I GCNNGC 8 cut(s) 60, 357, 871, 1771, 1804, 1852, 2109, 2113
CaiI CAGNNNCTG 1 cut(s) 619
CfoI GCGC 6 cut(s) 230, 1804, 1806, 1832, 2086, 2188
Cfr10I RCCGGY 2 cut(s) 1536, 2209
Cfr13I GGNCC 5 cut(s) 4, 668, 843, 1287, 1652
CpoI CGGWCCG 1 cut(s) 1652
CseI GACGC 2 cut(s) 1325, 1532
Csp6I GTAC 7 cut(s) 265, 917, 974, 1136, 1687, 1918, 1966
CspI CGGWCCG 1 cut(s) 1652
CviAII CATG 4 cut(s) 191, 280, 745, 1615
CviQI GTAC 7 cut(s) 265, 917, 974, 1136, 1687, 1918, 1966
DdeI CTNAG 3 cut(s) 1630, 1823, 2142
DraI TTTAAA 2 cut(s) 336, 2206
DrdI GACNNNNNNGTC 3 cut(s) 520, 1258, 1906
DseDI GACNNNNNNGTC 3 cut(s) 520, 1258, 1906
EaeI YGGCCR 2 cut(s) 1767, 2222
Eam1104I CTCTTC 4 cut(s) 791, 903, 1271, 1319
EarI CTCTTC 4 cut(s) 791, 903, 1271, 1319
Eco130I CCWWGG 1 cut(s) 1807
Eco147I AGGCCT 1 cut(s) 2140
Eco31I GGTCTC 2 cut(s) 324, 944
Eco47I GGWCC 3 cut(s) 668, 843, 1652
Eco57I CTGAAG 1 cut(s) 1695
Eco88I CYCGRG 1 cut(s) 1874
EcoRII CCWGG 5 cut(s) 6, 1289, 1481, 1728, 2160
EcoT14I CCWWGG 1 cut(s) 1807
ErhI CCWWGG 1 cut(s) 1807
Esp3I CGTCTC 1 cut(s) 1070
FaeI CATG 4 cut(s) 194, 283, 748, 1618
FalI AAGNNNNNCTT 2 cut(s) 362, 394
FaqI GGGAC 3 cut(s) 521, 1313, 1544
FatI CATG 4 cut(s) 190, 279, 744, 1614
FauI CCCGC 2 cut(s) 496, 1843
FauNDI CATATG 1 cut(s) 102
FblI GTMKAC 2 cut(s) 524, 653
FspBI CTAG 8 cut(s) 116, 476, 698, 773, 1284, 1487, 1670, 1940
GlaI GCGC 6 cut(s) 229, 1803, 1805, 1831, 2085, 2187
GsuI CTGGAG 6 cut(s) 29, 512, 1465, 1712, 1722, 1911
HaeII RGCGCY 1 cut(s) 2189
HaeIII GGCC 9 cut(s) 5, 137, 1289, 1769, 1821, 2042, 2111, 2140, 2224
HapII CCGG 5 cut(s) 1537, 1650, 1655, 2172, 2210
HgaI GACGC 2 cut(s) 1325, 1532
HhaI GCGC 6 cut(s) 230, 1804, 1806, 1832, 2086, 2188
Hin1II CATG 4 cut(s) 194, 283, 748, 1618
Hin6I GCGC 6 cut(s) 228, 1802, 1804, 1830, 2084, 2186
HinP1I GCGC 6 cut(s) 228, 1802, 1804, 1830, 2084, 2186
HincII GTYRAC 4 cut(s) 525, 654, 1116, 1958
HindII GTYRAC 4 cut(s) 525, 654, 1116, 1958
HindIII AAGCTT 1 cut(s) 1223
HpaII CCGG 5 cut(s) 1537, 1650, 1655, 2172, 2210
HphI GGTGA 2 cut(s) 1063, 1516
Hpy188I TCNGA 6 cut(s) 79, 86, 829, 1180, 1912, 1979
Hpy99I CGWCG 2 cut(s) 1260, 1916
HpyCH4III ACNGT 4 cut(s) 401, 1120, 1260, 1965
HpyCH4IV ACGT 3 cut(s) 1134, 1914, 2029
HpyF10VI GCNNNNNNNGC 5 cut(s) 352, 1818, 1827, 2104, 2230
HpyF3I CTNAG 3 cut(s) 1630, 1823, 2142
HpySE526I ACGT 3 cut(s) 1134, 1914, 2029
Hsp92II CATG 4 cut(s) 194, 283, 748, 1618
HspAI GCGC 6 cut(s) 228, 1802, 1804, 1830, 2084, 2186
Kpn2I TCCGGA 1 cut(s) 1649
LmnI GCTCC 3 cut(s) 493, 1363, 1484
LweI GCATC 2 cut(s) 651, 1581
MaeI CTAG 8 cut(s) 116, 476, 698, 773, 1284, 1487, 1670, 1940
MaeII ACGT 3 cut(s) 1134, 1914, 2029
MaeIII GTNAC 6 cut(s) 486, 563, 1153, 1355, 1544, 2267
MflI RGATCY 2 cut(s) 375, 1418
MhlI GDGCHC 3 cut(s) 827, 1481, 1856
MluCI AATT 8 cut(s) 173, 308, 383, 672, 863, 884, 993, 1184
MlyI GAGTC 7 cut(s) 128, 187, 503, 902, 1295, 1336, 1916
MmeI TCCRAC 1 cut(s) 1935
MroI TCCGGA 1 cut(s) 1649
MseI TTAA 8 cut(s) 14, 125, 165, 335, 707, 965, 1230, 2205
MslI CAYNNNNRTG 2 cut(s) 101, 912
MspA1I CMGCKG 2 cut(s) 349, 1160
MspCI CTTAAG 1 cut(s) 1229
MspI CCGG 5 cut(s) 1537, 1650, 1655, 2172, 2210
MspR9I CCNGG 6 cut(s) 8, 1291, 1483, 1730, 2162, 2173
Mva1269I GAATGC 2 cut(s) 505, 792
MvaI CCWGG 5 cut(s) 8, 1291, 1483, 1730, 2162
MvnI CGCG 4 cut(s) 58, 819, 1802, 1804
MwoI GCNNNNNNNGC 5 cut(s) 352, 1818, 1827, 2104, 2230
NciI CCSGG 1 cut(s) 2173
NdeI CATATG 1 cut(s) 102
NlaIII CATG 4 cut(s) 194, 283, 748, 1618
NlaIV GGNNCC 1 cut(s) 1679
NmeAIII GCCGAG 1 cut(s) 509
NmuCI GTSAC 2 cut(s) 563, 1544
NruI TCGCGA 2 cut(s) 58, 819
NspV TTCGAA 1 cut(s) 259
OliI CACNNNNGTG 1 cut(s) 912
PauI GCGCGC 1 cut(s) 1802
PceI AGGCCT 1 cut(s) 2140
PcsI WCGNNNNNNNCGW 1 cut(s) 55
PctI GAATGC 2 cut(s) 505, 792
PfeI GAWTC 3 cut(s) 141, 581, 1787
PflMI CCANNNNNTGG 2 cut(s) 677, 1568
PfoI TCCNGGA 1 cut(s) 1728
PleI GAGTC 7 cut(s) 127, 186, 503, 901, 1295, 1335, 1915
PpsI GAGTC 7 cut(s) 127, 186, 503, 901, 1295, 1335, 1915
PshAI GACNNNNGTC 2 cut(s) 1441, 2156
Psp6I CCWGG 5 cut(s) 6, 1289, 1481, 1728, 2160
PspGI CCWGG 5 cut(s) 6, 1289, 1481, 1728, 2160
PspN4I GGNNCC 1 cut(s) 1679
PspPI GGNCC 5 cut(s) 4, 668, 843, 1287, 1652
PsrI GAACNNNNNNTAC 2 cut(s) 1670, 1702
PstI CTGCAG 2 cut(s) 348, 354
PstNI CAGNNNCTG 1 cut(s) 619
PsuI RGATCY 2 cut(s) 375, 1418
PteI GCGCGC 1 cut(s) 1802
PvuII CAGCTG 2 cut(s) 349, 1160
RruI TCGCGA 2 cut(s) 58, 819
RsaI GTAC 7 cut(s) 266, 918, 975, 1137, 1688, 1919, 1967
RsaNI GTAC 7 cut(s) 265, 917, 974, 1136, 1687, 1918, 1966
RseI CAYNNNNRTG 2 cut(s) 101, 912
Rsr2I CGGWCCG 1 cut(s) 1652
RsrII CGGWCCG 1 cut(s) 1652
SalI GTCGAC 2 cut(s) 523, 652
SaqAI TTAA 8 cut(s) 14, 125, 165, 335, 707, 965, 1230, 2205
Sau96I GGNCC 5 cut(s) 4, 668, 843, 1287, 1652
SchI GAGTC 7 cut(s) 128, 187, 503, 902, 1295, 1336, 1916
ScrFI CCNGG 6 cut(s) 8, 1291, 1483, 1730, 2162, 2173
SduI GDGCHC 3 cut(s) 827, 1481, 1856
SfaNI GCATC 2 cut(s) 651, 1581
SfcI CTRYAG 2 cut(s) 344, 350
SfuI TTCGAA 1 cut(s) 259
SinI GGWCC 3 cut(s) 668, 843, 1652
SmiMI CAYNNNNRTG 2 cut(s) 101, 912
SmlI CTYRAG 2 cut(s) 1229, 2036
SmoI CTYRAG 2 cut(s) 1229, 2036
Sse9I AATT 8 cut(s) 173, 308, 383, 672, 863, 884, 993, 1184
SseBI AGGCCT 1 cut(s) 2140
SsiI CCGC 6 cut(s) 65, 503, 1850, 1931, 1953, 2225
SspMI CTAG 8 cut(s) 116, 476, 698, 773, 1284, 1487, 1670, 1940
StuI AGGCCT 1 cut(s) 2140
StyD4I CCNGG 6 cut(s) 6, 1289, 1481, 1728, 2160, 2171
StyI CCWWGG 1 cut(s) 1807
TaaI ACNGT 4 cut(s) 401, 1120, 1260, 1965
TaiI ACGT 3 cut(s) 1137, 1917, 2032
TaqII GACCGA 1 cut(s) 530
TasI AATT 8 cut(s) 173, 308, 383, 672, 863, 884, 993, 1184
TatI WGTACW 2 cut(s) 264, 916
TauI GCSGC 1 cut(s) 2227
TfiI GAWTC 3 cut(s) 141, 581, 1787
Tru1I TTAA 8 cut(s) 14, 125, 165, 335, 707, 965, 1230, 2205
Tru9I TTAA 8 cut(s) 14, 125, 165, 335, 707, 965, 1230, 2205
TscAI CASTG 4 cut(s) 1341, 1473, 1894, 2269
TseFI GTSAC 2 cut(s) 563, 1544
Tsp45I GTSAC 2 cut(s) 563, 1544
TspRI CASTG 4 cut(s) 1341, 1473, 1894, 2269
Van91I CCANNNNNTGG 2 cut(s) 677, 1568
Vha464I CTTAAG 1 cut(s) 1229
VpaK11BI GGWCC 3 cut(s) 668, 843, 1652
XapI RAATTY 1 cut(s) 863
XbaI TCTAGA 2 cut(s) 115, 1939
XcmI CCANNNNNNNNNTGG 1 cut(s) 1569
XmiI GTMKAC 2 cut(s) 524, 653
XspI CTAG 8 cut(s) 116, 476, 698, 773, 1284, 1487, 1670, 1940
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.